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ILE655VAL Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique Wulandari, Dwi; Azamris, Azamris; Nurhayati, Isna; Warisman, M Ali; Budiarto, Bugi Ratno; Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.104 KB) | DOI: 10.14203/ab.v21i2.310

Abstract

Transtuzumab has proven to be a great improvement in the treatment of HER2 + of breast cancer patients, but it is associated with relevant adverse cardiac events and with significantly elevated cost of treatment.  One of the risk factors for cardiotoxicity due to transtuzumab is the I655V HER2 polymorphism (GTC> ATC mutation) in which the allele mutant (Ile val or val / val) has a higher risk than the wild type (Ile/Ile). The detection of specific alleles is very important for therapeutic decision-making. In this study, our group have developed a HRM with EvaGreen dye method to discriminate specific allele of I655V HER2 (wild type, heterozygote mutant or homozygote mutant) in 66 frozen section samples of HER2+ of breast cancer patients. Our group reported the wild type is the most prevalent allele (77,27%), whereas heterozygous mutation is significantly present in this research (21,21%) and only 1,52% of sample detected as minor allele.  It showed that only one sample detected as a minor allele (val/val) and may have relatively low abundance in the population. This method has been compared with sanger sequencing and giving 100% of validity.
Conservation of Major L1 and Variability of Minor L2 Capsid Late Protein Genes in Human Papillomavirus of Indonesia Variants Nuswantara, Sukma; Prana, Titik K; Wulandari, Dwi; Widyowati, Henni; Anzela, Vera; Levy, Dea; Cahyadi, Petrus; Tjandra, Lukas D
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (898.192 KB) | DOI: 10.1234/50

Abstract

Human Papilloma Virus  (HPV) has an out standing feature for its vast intraspecies variability. Of all known 100 types or  more, 15 t ypes of them are classified as high risk  because of their occurrence  in  more than 95% of cervical cancer cases. Among all genes in their genome, E6 and E7 genes are considered oncogenes and have close relevance with their pathogenicity, whilst L1 and L2 ge nes produce capsid proteins that directly interact with their  host  receptors.  Considering  the  importance  of  L1  and  L2  in  host-receptor  relationship,  we  tried  to investigate their molecular variability thereby uncover their specificity as Indonesian variant s. Here we reported about the conservation of L1 minor capsid protein and variability of L2 capsid protein among high-risk types Human Papilloma Virus (HPV). The results indicated that L1 DNA was relatively more conserved than its L2 counterpart Also it was indicated that the middle part of either L1 or L2 CDS‟ showed more DNA variability than those  at  their  upstream  sequences.  It  is  concluded  that  L2  middle  sequences  are  important  factors  for intraspecific variations found in HPV of Indonesian variants.   Keywords: Human Papilloma Virus (HPV), L1 gene, L2 gene, cervical cancer, high risk HPV
Phylogenetic analysis and predicted functional effect of protein mutations of E6 and E7 HPV16 strains isolated in Indonesia Wulandari, Dwi; Rachmadi, Lisnawati; Sudiro, Tjahjani M.
Medical Journal of Indonesia Vol 24, No 4 (2015): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.88 KB) | DOI: 10.13181/mji.v24i4.1197

Abstract

Background: E6 and E7 are oncoproteins of HPV16. Natural amino acid variation in HPV16 E6 can alter its carcinogenic potential. The aim of this study was to analyze phylogenetically E6 and E7 genes and proteins of HPV16 from Indonesia and predict the effects of single amino acid substitution on protein function. This analysis could be used to reduce time, effort, and research cost as initial screening in selection of protein or isolates to be tested in vitro or in vivo.Methods: In this study, E6 and E7 gene sequences were obtained from 12 samples of  Indonesian isolates, which  were compared with HPV16R (prototype) and 6 standard isolates in the category of European (E), Asian (As), Asian-American (AA), African-1 (Af-1), African-2 (Af-2), and North American (NA) branch from Genbank. Bioedit v.7.0.0 was used to analyze the composition and substitution of single amino acids. Phylogenetic analysis of E6 and E7 genes and proteins was performed using Clustal X (1.81) and NJPLOT softwares. Effects of single amino acid substitutions on protein function of E6 and E7 were analysed by SNAP.Results: Java variants and isolate ui66* belonged to European branch, while the others belonged to Asian and African branches. Twelve changes of amino acids were found in E6 and one in E7 proteins. SNAP analysis showed two non neutral mutations, i.e. R10I and C63G in E6 proteins. R10I mutations were found in Af-2 genotype (AF472509) and Indonesian isolates (Af2*), while C63G mutation was found only in Af2*.Conclusion: E6 proteins of HPV16 variants were more variable than E7. SNAP analysis showed that only E6 protein of African-2 branch had functional differences compared to HPV16R.
EXAMINING STUDENTS’ NEEDS FOR ENGLISH AS REQUIRED COURSE IN DIPONEGORO UNIVERSITY Wulandari, Dwi
PAROLE: Journal of Linguistics and Education Volume 5 Number 1 April 2015
Publisher : Master Program in Linguistics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.867 KB) | DOI: 10.14710/parole.v5i1.84-94

Abstract

This study is aimed at finding out what students need in taking English as required course, what students want to learn, and how they want to learn. The data were taken from 500 questionnaires distributed to students. The data revealed that most of the students do have positive attitude in learning English, and because of this the students have more chances to take the best of their learning. However, as most of the students also take English because they are required to do so, there are also some factors that may discourage learners’ motivation in learning, i.e. materials selection, and teaching methods. Kajian ini ditujukan untuk mengetahui apa yang diinginkan oleh mahasiswa dalam mengambil mata kuliah MKDU Bahasa Inggris, secara spesifik untuk mengetahui materi apa yang mereka ingin pelajari, dan bagaimana mereka ingin mempelajarinya. Data diambil dari kuesioner yang disebarkan pada 500 mahasiswa. Analisis data menunjukkan bahwa sebagian besar siswa memiliki sikap bahasa yang positif  dalam mepelajari Bahasa Inggris, dan oleh karenanya mahasiswa memiliki kesempatan lebih banyak untuk mengambil keuntungan terbesar dari perkuliahan tersebut. Namun demikian, karena sebagian besar mahasiswa mengambil mata kuliah tersebut karena kewajiban, ada beberapa hal yang menyurutkan motivasi mahasiswa, yakni pemilihan materi, dan metode mengajar dosen  Bahasa Inggris.
TRANSPLANTASI LAMUN Thalassia hemprichii DENGAN METODE JANGKAR DI PERAIRAN TELUK AWUR DAN BANDENGAN, JEPARA Wulandari, Dwi; Riniatsih, Ita; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.767 KB) | DOI: 10.14710/jmr.v2i2.2347

Abstract

The seagrass bed is a coastal ecosystem which have an important role for the coastal environment. The seagrass beds are vulnerable with changes of water environmental conditions. The decrease of seagrass beds area in the world are the result from environmental stresses both of natural and impact of human activities. Transplantation is one way of to rehabilitate the condition of seagrass beds were damaged. The purpose of this research was to determine the survival rate and the rate of growth of transplanted seagrass Thalassia hemprichii with anchor method at Teluk Awur and Bandengan Waters Jepara. The method used in this research was field experimental method. Determination of sites using purposive random sampling method. The research was conducted at two stations are Teluk Awur Water as Station I and Bandengan Water as Station II. Each station divided into 3 plots of observation. The environmental parameters were taken are: salinity, temperature, current velocity, depth, nitrate, phosphate, dissolved oxygen, organic matter, composition and grain size of the substrate. The results showed that the survival rate of seagrass transplants at Teluk Awur water was higher when compared to the survival rate of seagrass transplants at Bandengan water. The survival rate of seagrass transplants at Teluk Awur water have a range of of 38,89% to 41,67%, while the survival rate of seagrass transplants at Bandengan water have a range of 20,97% to 23,15%. The average range growth rate of seagrass transplants at Teluk Awur water was 0,13 cm/day to 0,16 cm/day, while the average range growth rate of seagrass transplants at Bandengan water was 0.16 cm/day up to 0.17 cm/day.
PENERAPAN AKUNTANSI PADA DESA WISATA NGLINGGO KABUPATEN KULON PROGO Wulandari, Dwi; Widarno, Bambang; Suharno, Suharno
Jurnal Akuntansi dan Sistem Teknologi Informasi Vol 15, No 3 (2019): Akuntansi dan Sistem Teknologi Informasi
Publisher : Universitas Slamet Riyadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.399 KB)

Abstract

The program for the development and utilization of resources and the potential of regional tourism are expected to contribute to economic development. In order for tourism development to run optimally, good fund management is needed by applying accounting that is in accordance with the standards, namely the Financial Accounting Standards of Entities Without Public Accountability (SAK ETAP). This study aims to determine the application of accounting and help the preparation of financial statements in Nglinggo Tourism Village Kulon Progo Regency based on Financial Accounting Standards of Entities Without Public Accountability (SAK ETAP). This study uses qualitative research with a case study method. The data source used is secondary data. The data collection technique used is documentation and literature study. The data analysis technique uses descriptive analysis. The results of this study indicate that Nglinggo Tourism Village is a Tourism Village Management Agency engaged in services. Recording conducted by Nglinggo Tourism Village is manual and not computerized, which is used to determine the amount of profit and loss and the amount of salary costs and profit sharing costs so that it is still far from SAK ETAP. Based on this, the researchers sought to assist in the preparation of the financial report of Nglinggo Tourism Village in accordance with SAK ETAP, namely by preparing a system and accounting procedures based on the accounting cycle consisting of identifying stages, recording stages, classification stages, summarizing stages and reporting stages in the form of earnings reports loss and balance sheet. Based on the results of the financial statements that have been prepared, it shows that the income of Nglinggo Tourism Village is relatively large, where in 2017 the total income earned was Rp 281,819,000 with net income of Rp 65,608,792.
KOMUNIKASI TERAPEUTIK PERAWAT DENGAN TINGKAT KECEMASAN ANAK USIA PRASEKOLAH (3-5 TAHUN) SELAMA PEMASANGAN INFUS wulandari, dwi; ., fitriana
Journal of Nursing and Public Health Vol 5 No 2 (2017)
Publisher : UNIVED Press, Universitas Dehasen Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.386 KB) | DOI: 10.37676/jnph.v5i2.559

Abstract

Penyakit dan rawat inap sering menjadi isu krisis pada anak-anak. Selain itu, anak-anak yang dirawat di rumah sakit juga mendapat terapi infus yang sangat rentan terhadap kecemasan terkait dengan tindakan invasif. Selama pelaksanaan infus, seseorang sering merasa cemas terutama pada anak-anak karena takut akan tindakan seorang perawat. Komunikasi Terapeutik Perawat dengan Tingkat Kecemasan Anak Prasekolah (3-5 tahun) Selama Instalasi Infus di Instalasi Gawat Darurat Rumah Sakit Dr. M. Yunus Bengkulu pada tahun 2017. Metode penelitian yang digunakan adalah penelitian deskriptif dengan rancangan cross-sectional. Populasi adalah semua perawat yang ada di ruang Dr. DR. Rumah sakit M. Yunus Bengkulu pada 2017 sebanyak 27 perawat. Teknik sampling yang digunakan adalah teknik total sampling sehingga jumlah sampel sebanyak 27 orang. Hasil penelitian menunjukkan bahwa hampir separuh responden (40,7%) memiliki tingkat kecemasan yang parah, lebih dari separuh responden (59,3%), mendapat komunikasi terapeutik yang baik dari perawat dan ada hubungan antara komunikasi terapeutik perawat dengan tingkat kecemasan usia prasekolah (3-5 tahun) selama infus intravena di Unit Gawat Darurat Rumah Sakit Dr. M. Yunus Bengkulu pada tahun 2017 (p = 0,019). Dianjurkan ke rumah sakit Dr. M. Yunus Bengkulu untuk meningkatkan kualitas layanan keperawatan kepada anak sehingga anak-anak yang menjalani perawatan dapat menunjukkan kecemasan ringan atau tidak cemas dengan cara membuat peraturan kepada perawat untuk menerapkan pendekatan perawatan atraumatik pada anak-anak, terutama pada penerapan komunikasi terapeutik.
PENGARUH KONSUMSI AIR HANGAT TERHADAP FREKUENSI NAFAS PADA PASIEN ASMA DI PUSKESMAS SUKAMERINDU KOTA BENGKULU TAHUN 2019 HARDINA, SRI; ., SEPTIYANTI; WULANDARI, DWI
Journal of Nursing and Public Health Vol 7 No 2 (2019)
Publisher : UNIVED Press, Universitas Dehasen Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.884 KB) | DOI: 10.37676/jnph.v7i2.901

Abstract

Asma terjadi akibat gangguan pada sistem pernapasan yang menyebabkan penderita mengalami mengi (wheezing), sesak napas, batuk, dan sesak di dada tertama ketika malam hari atau dini hari. Tujuan penelitian ini adalah untuk mengetahui pengaruh konsumsi air hangat terhadap Frekuensi Nafas Pada pasien asma Di Puskesmas Sukamerindu Kota Bengkulu Tahun 2019. Metode yang digunakan adalah Quasi Eksperimen, two group test design with control group, sampel diambil sebanyak 24 orang dengan teknik purposive sampling. Hasil penelitian dengan analisis Uji Univariat Karakteristik penderita asma di Puskesmas Sukamerindu Kota Bengkulu yaitu 19 orang (67,9%) berjenis kelamin laki-laki, 22 orang (78,6%) berusia ≥19 tahun dan lama menderita asma 18 orang (64,3%) <5 tahun. Rata-rata frekuensi pernafasan pada kelompok kontrol sebelum (26,42) dan setelah (26,50), Rata-rata frekuensi pernafasan pada kelompok kasus sebelum (26,92) dan setelah (26,28). Hasil bivariat yaitu tidak ada pengaruh frekuensi nafas pada pasien asma sebelum dan setelah pada kelompok kontrol (tidak konsumsi air hangat), ada pengaruh frekuensi nafas pada pasien asma sebelum dan setelah pada kelompok intervensi (konsumsi air hangat). Ada pengaruh frekuensi nafas pada pasien asma kelompok post kasus dan kelompok post kontrol di Puskesmas Sukamerindu Kota Bengkulu Tahun 2019 Peneliti menyarankan pihak Puskesmas diharapkan dapat memberikan penyuluhan tentang manfaat konsumsi air hangat sebagai terapi alternative pada pengobatan asma.
PENGARUH DZIKIR TERHADAP SKALA NYERI PADA IBU POST PARTUM SECTIO CAESAREA DI RSUD DR. M. YUNUS BENGKULU TAHUN 2019 VIVIYANI, TIARA; WULANDARI, DWI; RAHMADANI, ELSI
Journal of Nursing and Public Health Vol 7 No 2 (2019)
Publisher : UNIVED Press, Universitas Dehasen Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.117 KB) | DOI: 10.37676/jnph.v7i2.903

Abstract

Sectio Caesarea di Indonesia persentasenya sebesar15,3%, di Provinsi Bengkulu sectio caesar sebanyak 33.854 orang serta pada tahun 2018 sebanyak 393 orang di RSUD Dr. M.Yunus Bengkulu. Tujuan penelitian ini adalah untuk mengetahui pengaruh dzikir terhadap skala nyeri pada ibu post partum Sectio Caesarea di RSUD Dr. M. Yunus Bengkulu Tahun 2019. Metode yang digunakan adalah Quasi Eksperimen, pre two group test design with control group, data dikumpulkan melalui observasi skala nyeri sectio caesar pada 26 orang sampel dengan teknik accidental sampling. Hasil analisis Uji Univariat karakteristik ibu post partum yang melakukan sectio caesar yaitu 15 orang (57,7%) berumur <20 tahun dan 12 orang (46,2%) berpendidikan SMA. Rata-rata skala nyeri sebelum pada ibu post partum Sectio Caesarea pada kelompok intervensi 4,84 (Nyeri sedang) nyeri setelah 1,85 (Nyeri ringan). Rata-rata skala nyeri sebelum dan setelah pada ibu post partum Sectio Caesarea yaitu 5,00 (Nyeri sedang). Hasil bivariat yaitu Tidak ada pengaruh penurunan nyeri sebelum dan setelah pada ibu post partum Sectio Caesarea pada kelompok kontrol (tidak dilakukan dzikir) . Ada pengaruh skala nyeri pada ibu post partum Sectio Caesarea pada kelompok intervensi (dilakukan dzikir) di RSUD Dr. M. Yunus Bengkulu Tahun 2019 dan tidak ada pengaruh penurunan nyeri sebelum dan setelah pada ibu post partum Sectio Caesarea pada kelompok kontrol (tidak dilakukan dzikir). Peneliti menyarankan pihak Rumah Sakit pihak petugas dapat menerapkan terapi dzikir dalam menurunkan nyeri post sectio caesar bukan hanya menjadi anjuran tapi melaksanakan intervensi terhadap anjuran yang selama ini diberikan.
Impact of Problem-Based Learning on Student Achievement in Economics Course Narmaditya, Bagus Shandy; Winarning, Winarning; Wulandari, Dwi
Classroom Action Research Journal (CARJO) Vol 1, No 1 (2017)
Publisher : Unversitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study investigated and understand impact of Problem-Based Learning on students’ achievement in the economic course through lesson study. This kind a classroom action research in the subject of money and bank in order to participate in solving problems related materials. The Subject of this study is Grade 9 student in academic year 2016/2017. The implementation classroom action research conducted in two cycles comprising Planning, Implementation, observing, and reflection stages. The result of this study that Problem-Based Learning through lessons has been well implemented including the completeness of the learning tools and preparing the Lesson Plans before teaching on each teaching material. Based on the results of the implementation of lesson study using Problem-Based Learning model can be concluded that Problem-Based Learning through on the learning can improve student achievement.Keywords: Problem-Based Learning, Student Achievement, Classroom Research DOI: 10.17977/um013v1i12017p001
Co-Authors ., SEPTIYANTI Abdullah, Sofwan Siddiq Adetya, Nais Pinta Adinata, Aqilla Tri Adiyaksa, Ivan Khofian Affero Ismail, Affero Afif Noor Afwan Hariri Agus Hermanto Ahmad Fauzan Alfiana, Serefina Alfiota, Syf. Ine Ali Mustofa, Triono Alifia Revan Prananda Altitania, Rizky Amar, Rizki Oktavianti Andi Basuki, Andi Andini, Riky Cahya Andri Eko Putra, Andri Eko Anggraeni, Sefira Oktavia Annisa, Rahma Anzela, Vera Arbin Janu Setiyowati, Arbin Ari Gunawan Arido Laksono Arsiana, Dian Aruman, Achmad Edhy Aruna, Alby Asfan, Muhammad Asmawati Asmawati Astuti, Melina Yuni Ayunin, Qurrota Ayunin, Salsabella Qurrota Azamris, Azamris Bagus Shandy Narmaditya Bambang Widarno Bangun, Ernalem Bayu Saputra BUGI RATNO BUDIARTO Bulu, Atalia Tamo Ina Buyung Adi Dharma Cahayati, Nila Cahyadi, Petrus Candria, Mytha Desriani Desriani Dewati, Niken Ayu Dewi, Dinie Anggraeni Dian Septi Nur Afifah Dinata, Vania Mitzi Djunaidi Djunaidi Dwi Faqihatus Syarifah Has Effendi, Darwin Eka Legya Frannita Ella Nurlaella Hadi Ervia Yudiati Fajariant, Otto Fikri Aulia Firdaus, Zulfan Al Fitriana . Fuad Indra Kusuma Furi Furnamasari, Yayang Gita Mulyasari Grisvia Agustin Halim, Muhammad Arsyad HARDINA, SRI Hasnan, Haikal Haya, Aqilla Fadia Heltty Heltty Hendri Hermawan Hendriani, Lis Henny Henny, Henny Heru Budi Susanto, Heru Budi Husna, Inayatul I Ketut Sudiana Imam Mukhlis Imawati, Sri Indra, Rani Lisa Indriati, Nur Isna Nurhayati, Isna Ita Riniatsih J.P, Chelsea Karima, Ummul Kartika, Defi Eka Kartini, Dewi Kurniawati, Rizki Dwi Kusuma, Kusuma Lestari, Mas roro Diah Wahyu Levy, Dea Linda Seprillina Lisnawati Rachmadi Llego, Jordan Lubis, Dina Mariana Uli Lutfiyah, Nanik M. Chaerul Rizky Mahadewi, Kirana Marcelliantika, Adinda Maryam, Sinta Dewi Maulana, Afnan Barkah N. G. A. A. S. Melinda, Meisya Meriyati Meriyati Miftahul Hasanah, Miftahul Mohamad Andrie Munparidi Ningrum, Linda Agustin Nur Elly Nur Hayati Nurohmah, Ai Nurul Nursilawati, Nursilawati Nurwantoro Oktariansyah Oktariansyah, Oktariansyah Otto Fajarianto P.A., Ageng Palupi, Dyah Hasto Pebriani, Linda Prana, Titik K Prasetyo, Abdul Rahman Prasetyo, Didi Yudha Priamsari, Margaretha Retno Prihantini Prihantini Pujo Widodo, Pujo Purnama, Ningtias Putra Hilmi Prayitno Putra, Hilmy Aditama Surya Putri, Naimah RAHAYU WIDYASTUTI Rahmadani, Elsi Rahmawati, Atiqa Rahyu Setiani Ramdhani, Hikmat Rani, Maesa Rani, Maesa Rasyid, T. Abdur RATNADHIYANI, ANDITHA Regilsa, M. Reswita Reswita, Reswita Retno Wulandari Riyadi, Eirene Yonastasia Rizki Firmansyah Robbika, Fadzkurisma Rosita Sabilla, Annisa Safitri, Andriani Safitri, Ramdani Sandra Sandra Santi, Dhira Saputra, Miftahul Hilda Saputri, Dela Refi Novi Sawal, Rizky Ardian Hartanto Selamet Joko Utomo, Selamet Joko Septiyanti, Septiyanti SIMAMORA, SERLI FEBRIANI Siti Jamilah Situmorang, Anggi Ardani Br Subagyo Subagyo Sudarno Sudarno Sukma Nuswantara Sukma, Muhammad Abi Surya, Eka Putri Suwarno, Panji Syafruddin, Afis Baghiz Syaifruddin, Afis Baghiz Syarifa, Najwa Kamila Thomas Soseco Tjahjani M. Sudiro Tjandra, Lukas D Tranggana, Wisnu Lintang Tri Agustina, Tri Triana Lestari Trnmiyati, Eko Valentine, Fatresia Vidya Purnamasari Vivekanantharasa, Raveenthiran VIVIYANI, TIARA Wahyuni, Khusniyatul Waliyanti, Ema Warisman, M Ali Widia Lestari Widyowati, Henni Wijaya, Andra Saferi Wijaya, Ginanjar Atma Wikan Budi Utami Winarning Winarning, Winarning Wintari Taurina Wulandari, Widia Ayu Yuliati, Luluk Yuningsih, Neni Misna Yusuf Tri Herlambang, Yusuf Tri Zahra, Mutiah Az Zuhud, Evrizal A M Zulfi Hendri