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All Journal Jurnal Pendidikan Matematika dan IPA Prosiding Seminar Biologi AGRIVITA, Journal of Agricultural Science Sawwa: Jurnal Studi Gender ANNALES BOGORIENSES AGROTEK: Jurnal Ilmiah Ilmu Pertanian Jurnal Sains dan Teknologi Pertanian Proceeding Biology Education Conference Makara Journal of Health Research Jurnal El-Thawalib
Desriani Desriani
Research Centre for Biotechnology, LIPI. Jl. Raya Bogor Km 46, Kec. Cibinong, Kab. Bogor, 16911. Indonesia. Telp: +62-21-8754587.
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Editorial Boards AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.296 KB) | DOI: 10.14203/ab.v21i1.304

Abstract

Editors Preface AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.21 KB) | DOI: 10.14203/ab.v21i1.305

Abstract

Guide for Authors AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.473 KB) | DOI: 10.14203/ab.v21i1.306

Abstract

Improvement of HER2I655V TARMS-PCR Performance by DNA Quality Analysis Budiarto, Bugi Ratno; Azamris, Azamris; Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v21i2.308

Abstract

Reliable TARMS-PCR is a prerequisite in constructing a solid conclusion in genetic diagnostics. The validity of data generated by this molecular technique is hampered by a false positive result. In attempt to develop a  TARMS-PCR for HER2I655V genotyping with no interfering of bias we used DNase I to eliminate DNA contaminant resided in PCR reagent. TARMS-PCR without enzyme treatment using recombinant plasmids that contained HER2I655V gene with represented its alleles was used to evaluate the presence of false positive  result while DNase I treated-PCR reagent was used in TARMS-PCR to evaluate the effective dose of the enzyme and further to adjust the TARMS-PCR conditions.  PCR master mix kit used in this study produced a false positive result on HER2I655V TARMS-PCR as proven by the presence of multiple PCR products in Non-Template Control (NTC) and 0.1 U of the enzyme could eliminate this DNA contaminant effectively, although this pretreatment altered the specificity of HER2I655V TARMS-PCR genotyping on certain genotype. Combination of touchdown TARMS-PCR with another allele-specific primer recovered specificity of detection on this model system. Interestingly, this optimized HER2I655V TARMS-PCR can only be used for genotyping the clinical samples if only further optimization was done using genomic DNA as template
Front Cover AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (774.26 KB) | DOI: 10.14203/ab.v21i1.303

Abstract

ILE655VAL Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique Wulandari, Dwi; Azamris, Azamris; Nurhayati, Isna; Warisman, M Ali; Budiarto, Bugi Ratno; Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.104 KB) | DOI: 10.14203/ab.v21i2.310

Abstract

Transtuzumab has proven to be a great improvement in the treatment of HER2 + of breast cancer patients, but it is associated with relevant adverse cardiac events and with significantly elevated cost of treatment.  One of the risk factors for cardiotoxicity due to transtuzumab is the I655V HER2 polymorphism (GTC> ATC mutation) in which the allele mutant (Ile val or val / val) has a higher risk than the wild type (Ile/Ile). The detection of specific alleles is very important for therapeutic decision-making. In this study, our group have developed a HRM with EvaGreen dye method to discriminate specific allele of I655V HER2 (wild type, heterozygote mutant or homozygote mutant) in 66 frozen section samples of HER2+ of breast cancer patients. Our group reported the wild type is the most prevalent allele (77,27%), whereas heterozygous mutation is significantly present in this research (21,21%) and only 1,52% of sample detected as minor allele.  It showed that only one sample detected as a minor allele (val/val) and may have relatively low abundance in the population. This method has been compared with sanger sequencing and giving 100% of validity.
Back Cover AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.912 KB) | DOI: 10.14203/ann.bogor.2017.v21.n1.%p

Abstract

DETEKSI ENZIM CELLOBIOSE DEHYDROGENASE (CDH) DARI FUNGI TRAMETES VERSICOLOR Desriani, Desriani; Falah, Syamsul; Bintang, Maria; Kusumawati, Dwi Endah; Amrinola, Wiwit; Hasanah, Neneng
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.247 KB)

Abstract

Trametes versicolor merupakan salah satu fungi pendegradasi kayu dari golongan white rot fungi yang memiliki kemampuan dalam menghasilkan enzim ekstraseluler, yaitu cellobiose dehydrogenase (CDH). Enzim CDH memiliki dua domain, yaitu domain flavin dan domain heme yang dihubungkan oleh 25 asam amino yang kaya akan serin dan treonin. Penelitian ini bertujuan mendeteksi CDH yang dihasilkan oleh T. versicolor baik secara kualitatif maupun kuantitatif. Deteksi CDH dilakukan melalui empat  tahap, yaitu: uji kualitatif enzim ekstraseluler, produksi CDH pada media cair, pengukuran aktivitas CDH menggunakan DCIP sebagai akseptor elektron dan selobiosa sebagai substrat, serta analisis bobot molekul CDH dengan elektroforesis SDS-Page. Hasil penelitian menunjukkan terbentuknya zona bening disekitar fungi setelah perlakuan congo red dan NaCl yang mengindikasikan bahwa T. versicolor menghasilkan enzim ekstraseluler. Produksi CDH dilakukan dengan menumbuhkan fungi di media YPD dan media minimal guna menginduksi produksi CDH. Pengukuran aktivitas enzim CDH belum berhasil dilakukan dan berdasarkan hasil SDS-PAGE, sebagian besar enzim CDH dari T. versicolor dihasilkan dalam bentuk domain flavin.  Kata kunci : Cellobiose dehydrogenase, SDS-Page, Trametes versicolor
The Sex Education Method in Agrarian Communities Desriani, Desriani; Muthohar, Sofa; Filasofa, Lilif Muallifatul Khorida; Mursid, Mursid
Sawwa: Jurnal Studi Gender Vol 16, No 1 (2021): April
Publisher : Pusat Studi gender dan Anak (PSGA) Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.393 KB) | DOI: 10.21580/sa.v16i1.6087

Abstract

The purpose of this study was to describe how an agrarian society provides sex education. This research was conducted based on the assumption that parents need to teach sex education to early childhood, even though it is taboo, but in a good way. This research is qualitative research with an ethnographic research type. The results showed that 1) The community uses fiqh (rituals of worship) learning methods to cover genitalia and teach the culture of shame from an early age. 2) people are still taboo to mention genitals directly so that they use other terms that are considered not to stimulate crime and are more polite, namely to refer to the penis as sunik, gentog and anu, while for the vagina is replaced with the term memek, iwak kebo and anu. 3) people still use the circumcision method for women even though WHO has stated it is prohibited. This study recommends the government and community leaders to continue to carry out sex education for children with the correct method according to health, religious, and human rights laws.
DETEKSI ENZIM CELLOBIOSE DEHYDROGENASE (CDH) DARI FUNGI Trametes versicolor Desriani, Desriani; Falah, Syamsul; Bintang, Maria; Kusumawati, Dwi Endah; Amrinola, Wiwit; Hasanah, Neneng
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Trametes versicolor merupakan salah satu fungi pendegradasi kayu dari golongan white rot fungi yang memiliki kemampuan dalam menghasilkan enzim ekstraseluler, yaitu cellobiose dehydrogenase (CDH). Enzim CDH memiliki dua domain, yaitu domain flavin dan domain heme yang dihubungkan oleh 25 asam amino yang kaya akan serin dan treonin. Penelitian ini bertujuan mendeteksi CDH yang dihasilkan oleh T. versicolor baik secara kualitatif maupun kuantitatif. Deteksi CDH dilakukan melalui empat  tahap, yaitu: uji kualitatif enzim ekstraseluler, produksi CDH pada media cair, pengukuran aktivitas CDH menggunakan DCIP sebagai akseptor elektron dan selobiosa sebagai substrat, serta analisis bobot molekul CDH dengan elektroforesis SDS-Page. Hasil penelitian menunjukkan terbentuknya zona bening disekitar fungi setelah perlakuan congo red dan NaCl yang mengindikasikan bahwa T. versicolor menghasilkan enzim ekstraseluler. Produksi CDH dilakukan dengan menumbuhkan fungi di media YPD dan media minimal guna menginduksi produksi CDH. Pengukuran aktivitas enzim CDH belum berhasil dilakukan dan berdasarkan hasil SDS-PAGE, sebagian besar enzim CDH dari T. versicolor dihasilkan dalam bentuk domain flavin.  Kata kunci : Cellobiose dehydrogenase, SDS-Page, Trametes versicolor
Co-Authors Azamris, Azamris Budiarto, Bugi R BUGI RATNO BUDIARTO Chaeriyah, Naeilul Dwi Endah Kusumawati Dwi Wulandari Filasofa, Lilif Muallifatul Khorida Hapsari, Betalini Widhi Harahap, Risalan Basri Harahap, Wirsma A Hasibuan, zulfan Efendi Haz, Aufa Rizqia Isna Nurhayati, Isna Jumardi Jumardi Kumala, Ajeng Putri MARIA BINTANG Marti Abna, Inherni Munawarah Munawarah, Munawarah Mursid Mursid Neneng Hasanah Noorohmah, Siti Okasa, Andi Muliarni Putri Kumala, Ajeng Rahayu, Resa Sri Raihan, Eldrian Daffa Rantau, Deritha Ellfy Rinal Effendi Siti Zubaidah Sitti Fatimah Syahid Sofa Muthohar Sulaiman Sulaiman Syamsul Falah Warisman, M Ali Wiwit Amrinola Wulandari, Dyah Retno Yuliawati Yurika, Oktri