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All Journal 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Jurnal Analis Medika Biosains (JAMBS) Jurnal Ilmiah Tatengkorang Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat
Mutiarawati, Diah Titik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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ANALISIS MIKROBA JUMLAH ANGKA LEMPENG TOTAL PADA IKAN CAKALANG (Katsuwonus pelamis) DI PASAR GEDANGAN SIDOARJO mutiarawati, diah titik
Analis Kesehatan Sains Vol. 12 No. 2 (2023): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/anakes.v12i2.8

Abstract

Ikan cakalang adalah salah satu jenis ikan pelagis dengan kandungan protein tinggi. Kandungan air yang cukup tinggi pada tubuh ikan menjadi media yang cocok untuk kehidupan bakteri dan mikroorganisme lain serta daging ikan memiliki sedikit tendon, sehingga proses pembusukan akibat aktivitas bakteri lebih cepat dan mutu ikan berkurang serta tidak layak konsumsi, dimana untuk mengetahui adanya cemaran mikroba pada pangan dengan pemeriksaan mikrobiologis yaitu menguji total bakteri pada ikan cakalang yang dihitung berdasarkan Angka Lempeng total (ALT). Penelitianinibertujuanmengetahuijumlahmikrobapadaikancakalang (Katsuwonus pelamis) yang dijual dipasar Gedangan Sidoarjo. Jenis penelitian ini adalah deskripsi dan penelitian dilakukan di Laboratorium Mikrobiologi Jurusan Analis Kesehatan Politekknik Kesehatan Surabaya. Sampel penelitian adalah ikan cakalang sebanyak 5 buah yang diambil secara total sampling. Metode pemeriksaan menggunakan hitung cawan dengan metode tuang. Hasil penelitian menunjukkan bahwa pengujian Angka Lempeng Total pada ikan cakalang (Katsuwonus pelamis) yang dijual dipasar Gedangan Sidoarjo setelah dilakukan uji secara mikrobiologi yaitu dari ke lima sampel yang dilakukan analisis terdapat 4 sampel yang termasukdalambatasnormalyangsesuaidenganStandarNasionalIndonesia(SNI)7388:2009 tentang batas maksimum mikroba dalam pangan yaitu 5x105koloni/g yang artinya ikan tersebut aman untuk dikonsumsi. Sedangkan 1 sampel termasuk dalam cemaran, karena tidak sesuai dengan Standart Nasional Indonesia (SNI) 7388:2009 tentang batas maksimum cemaran mikroba dalam pangan yaitu 5x105koloni/g yang artinya ikan tersebut tidak boleh untuk dikonsumsi berdasarkan Standart Nasional Indonesia (SNI) 7388:2009 tentang batas maksimum cemaran mikroba dalampangan.  
Kolesterol dan Tekanan Darah Lansia di Posyandu Abiyoso Polkesbaya Christyaningsih, Juliana; Rusyadi, Luthfi; Minarti, Minarti; Mamik, Mamik; Pestariati, Pestariati; Mutiarawati, Diah Titik; Suliati, Suliati; Sasongkowati, Retno; Woelansari, Evy Diah; Suhariyadi, Suhariyadi; Istanto, Wisnu; Endarini, Lully Hanni; Museyaroh, Museyaroh
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 2 No 2 (2023): Jurnal Rakat Sehat: Pengabdian Kepada Masyarakat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v2i2.35

Abstract

As people age, the physical state of the human body will gradually deteriorate due to a decrease in the quantity of cells present, leading to a gradual reduction in functionality referred to as the aging process. In elderly humans (aged more than 60 years) excessive cholesterol levels and high blood pressure are one of the causes of diseases such as heart disease which has been recognized by WHO as the number one cause of death in the world. The buildup of fatty deposits on the linings of blood vessels has the potential to lead to obstructions within the blood vessels. The objective of this initiative is to enhance understanding regarding the hazards associated with cholesterol. This outreach effort took place in July 2023, conducted at Posyandu Abiyoso, Surabaya Health Polytechnic. The activity consists of several processes 1). conducting a survey of the location of the Abiyoso posyandu of the Surabaya Health Polytechnic, 2). Opening registration and taking participants' personal data, 3) conducting participant health interviews, 4). Checking cholesterol levels and blood pressure of participants. From the community service activities carried out, it was found that 42 of the 65 (64.6%) participants who took part in the examination at the integrated service post had high cholesterol levels, and 60 of the 65 (92.3%) participants had high blood pressure or hypertension. By conducting health checks on cholesterol levels and blood pressure, there is an expectation that the society will come to realize and comprehend. the dangers of hypertension and hypercholesterolemia so that the community can prevent the occurrence of sustainable diseases.
AKSI KOMPETENSI BERSAMA DI PANTI ASUHAN MISI NUSANTARA SURAKARTA Juliana Christyaningsih; Puspitadewi, Teresia Retna; Alberta, Lembunai Tat; Pengge, Nuning Marina; Siagian, Hotmaida; Mutiarawati, Diah Titik; Rahayuningsih, Christ Kartika; Kurniawan, Dedi; Lestari, Indah; Sumasto, Hery; Endarini, Lully Hani; Kiaonarni OW; Ira Puspita Sari; Evi Yunita N; Suliati; Darjati; Nur Hatijah; Khambali; Hadi Suryono; Demes Nurmayanti; Tatarini Ika Pipitcahyani; Astuti Setiyani; Siti Alfiah; Dina Isfentiani; Bambang Hadi Sugito; Retno Sasongkowati; Yusianti Silviani; N. S. Widodo
Jurnal Ilmiah Tatengkorang Vol 7 No 1 (2023): Jurnal Ilmia Tatengkorang
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Politeknik Negeri Nusa Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54484/tkrg.v7i1.553

Abstract

Permasalahan sosial yang banyak terjadi di masyarakat adalah kaum marjinal yang terpinggirkan seperti anak terlantar, anak jalanan, pengemis, dan sebagainya. Salah satu upaya mengatasi masalah kaum marjinal yaitu dengan didirikan lembaga sosial berupa panti asuhan anak. Sejak masa pandemi, anak di Panti Asuhan Misi Nusantara Surakarta jarang mendapat layanan kesehatan dan ada keterbatasan ruang gerak anak-anak. Tujuan pengabdian masyarakat ini untuk membantu panti asuhan dalam memantau pertumbuhan dan perkembangan penghuni dan pengelola Panti Asuhan Misi Nusantara di Surakarta. Metoda pelaksanaan berupa pemeriksaan tumbuh kembang anak, Penyuluhan Hidup Bersih dan Sehat, pemeriksaan Status Gizi Anak, penyuluhan cara menyikat gigi yang benar, pemeriksaan kesehatan pengelola panti asuhan serta pelatihan penanggulangan bencana. Hasil pemeriksaan Kesehatan pada penghuni dan pengelola panti didapatkan sebagian besar (52,6 persen) penghuni panti berjenis kelamin laki-laki, hampir setengahnya berusia rata-rata 21-60 tahun, Sebagian besar (68,4 persen) tekanan darah kurang dari 140/90 mmHg, Sebagian besar (73,1 persen) berstatus gizi normal, hampir seluruhnya (94,4 persen) glukosa darah kurang dari 126 m/dL dan asam urat kurang dari 8 mg/dL. Secara umum keadaan warga panti asuhan dalam keadaan baik Social problems that often occur in society are abandoned children, street children, beggars, disabled people, neglected elderly, poor families, families with social problems, and so on. Children and poor people should have their human rights fulfilled by the State of Indonesia. However, in reality there are many children whose needs are not met, such as parents who are unable to provide for the child's needs or the child does not have parents. One effort to overcome this is to establish social institutions in the form of orphanages. Since the pandemic, children at the Mission Nusantara Surakarta Orphanage rarely receive health services and there is limited space for children to move. Theless than purpose of this community service is to assist the orphanage in monitoring the growth and development of residents and managers of the Mission Nusantara Orphanage in Surakarta. The implementation method is in the form of examinations on child growth and development, Counseling on Clean and Healthy Living, checking on the Nutritional Status of Children, counseling on how to brush their teeth properly, health checks for orphanage managers and training on disaster management. 6 percent) of the residents of the orphanage are male, almost half of them are aged 21-60 years, most (68.4 percent) have blood pressure less than 140/90 mmHg, most (73.1 percent) have normal nutritional status, almost all (94.4 percent) blood glucose less than 126 m/dL and uric acid less than 8 mg/dl. Similar activities are needed to see general body health and must be monitored periodically, so that the residents of the orphanage can find out their health status, especially now that they are still in a pandemic condition Covid-19
The Relationship Between Blood Glucose Levels To The Results Mycobacterium Tuberculosis Examination In Tuberculosis Patients Puspita, Ria; Suliati, Suliati; Mutiarawati, Diah Titik
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.330

Abstract

Tuberculosis (TB) is still high. 10.4 million new cases of TB occur and 1.4 million die from the disease is found The World Health Organization (WHO). Elevated blood glucose can lead to more severe tuberculosis infection, reactivation of dormant tuberculosis foci, and poor treatment outcomes. Determine the relationship of blood glucose levels to the results of Mycobacterium tuberculosis examination in suspected tuberculosis patients in Ketapang Banyuwangi area is the purpose of this of research study. The method was correlational analytic design of this study. The results conducted on 118 people who performed tuberculosis screening examinations, 32 people who qualified as a population. And 30 (thirty) people who meet the inclusion requirements as research samples. The results of the patient's molecular rapid test showed 6 (six) people were positive (+), and the results of the patient's culture showed 3 (three) people were positive (+) and 3 (three) other people were negative (-). While the results of fasting blood glucose level examination in 3 (three) tuberculosis patients conducted periodically (every week) for 5 (five) weeks were in the range of 78mg/dL - 150mg/dL. The conclusion is that there is a significant relationship between blood glucose levels and results of Mycobacterium tuberculosis examination of tuberculosis patients in the first, second and third patients in Ketapang Banyuwangi area.
Media Alternatif Agar Jagung untuk Identifikasi Candida albicans Junaedi, Winda Rachmawati; Suliati, Suliati; Mutiarawati, Diah Titik; Endarini, Lully Hanni
2-TRIK: TUNAS-TUNAS RISET KESEHATAN Vol 14, No 3 (2024): Juli-September 2024
Publisher : FORUM ILMIAH KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/2trik14304

Abstract

Fungal culture media is the gold standard for diagnosing candidiasis. Generally, the media used are ready-to-use preparations, as an alternative medium for the identification of Candida albicans. The carbohydrate content in the form of starch contained in the mixed corn agar media provides a source of nutrition for growth and development. The addition of Tween 80 to the mixed corn agar media under oxygen-deficient culture conditions (caused by the inoculum with a cover glass) creates an unfavorable environment and stimulates the formation of chlamydospores (a microscopic characteristic of Candida albicans). The purpose of this study was to determine the sensitivity of mixed corn agar media as an alternative medium for the identification of Candida albicans. Examination of the macroscopic characteristics of Candida albicans was carried out by inoculating a Candida albicans suspension on mixed corn agar media and cornmeal media (control). The study was conducted from December 2019 to June 2020 at the Clinical Microbiology Laboratory, Surabaya Center for Health Laboratory. Based on the results of the examination and data analysis using the Kruskal-Wallis test, the p-value was 0.368. Therefore, there were no differences in the macroscopic characteristics of Candida albicans on the three media: mixed corn agar, mixed rice agar, and cornmeal (positive control). Therefore, it can be concluded that mixed corn agar and mixed rice agar are sensitive alternative media for the identification of Candida albicans.Keywords: Candida albicans; mixed corn agar; cornmeal; macroscopic characteristicsABSTRAK Media dalam pemeriksaan kultur jamur merupakan baku emas untuk penegakan diagnosa kandidiasis. Pada umumnya media yang digunakan sudah dalam bentuk sediaan siap pakai, sebagai media alternatif untuk identifikasi Candida albicans. Kandungan karbohidrat berupa zat pati yang terdapat pada media agar jagung racikan menjadi sumber nutrisi untuk tumbuh dan berkembang. Penambahan tween 80 pada media agar jagung racikan dengan kondisi kultur kekurangan oksigen (disebabkan inoculum dengan kaca penutup) menciptakan lingkungan yang kurang baik dan merangsang pembentukan klamidospora (karakteristik mikroskopis Candida albicans). Tujuan penelitian ini adalah untuk mengetahui sensitivitas media agar jagung racikan sebagai media alternatif untuk identifikasi Candida albicans. Pemeriksaan karakteristik makroskopis Candida albicans dilakukan dengan cara menginokulasikan suspensi Candida albicans pada media agar jagung racikan, dan media cornmeal (kontrol). Penelitian dilakukan pada bulan Desember 2019 sampai  Juni 2020 di Laboratorium Mikrobiologi Klinik, Balai Besar Laboratorium Kesehatan Surabaya. Berdasarkan hasil pemeriksaan dan analisis data dengan uji Kruskal Wallis diketahui bahwa nilai p adalah 0,368. Dengan demikian, tidak ada perbedaan karakteristik makroskopis Candida albicans pada ketiga media yaitu agar jagung racikan, media agar beras racikan dan media cornmeal (kontrol positif). Dengan demikian bisa disimpulkan bahwa media agar jagung racikan dan media agar beras racikan dapat dikatakan sensitif sebagai media alternatif untuk identifikasi Candida albicans.Kata kunci: Candida albicans; media agar jagung racikan; media cornmeal; karakteristik makroskopis 
Co-Authors Astuti Setiyani Bambang Hadi Sugito Darjati Dedi Kurniawan Demes Nurmayanti Endarini, Lully Hani Endarini, Lully Hanni Evi Yunita N Evy Diah Woelansari Hadi Suryono Indah Lestari, Indah Ira Puspita Sari Isfentiani, Dina Istanto, Wisnu Juliana Christyaningsih Junaedi, Winda Rachmawati Khambali, Khambali Kiaonarni OW Lembunai Tat Alberta, Lembunai Tat Luthfi Rusyadi Mamik Mamik Minarti Minarti Museyaroh, Museyaroh N. S. Widodo Nur Hatijah Pengge, Nuning Marina Pestariati, Pestariati Puspita, Ria Puspitadewi, Teresia Retna Rahayuningsih, Christ Kartika Retno Sasongkowati Sasongkowati, Retno Siagian, Hotmaida Siti Alfiah Suhariyadi Suhariyadi Suliati Suliati Suliati Sumasto, Hery Yusianti Silviani