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All Journal International Journal of Public Health Science (IJPHS) Journals of Ners Community JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal EduHealth Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat
Pestariati, Pestariati
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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UJI SENSITIVITAS PROPOLIS TERHADAP BAKTERI Staphylococcus aureus Dan Eschericia coli Anindya, Lokawanti Al Fahna; Pestariati, Pestariati; Wisnu, Istanto
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/anakes.v10i2.18

Abstract

The threat of infectious diseases from strains of pathogenic bacteria Staphylococcus aureus and Eschericia coli is capable of causing resistance to antibiotics. The prevalence of MRSA cases was 8.2%. One of the efforts to control infections caused by antibiotic-resistant bacteria is to utilize several antimicrobial compounds derived from natural ingredients, namely propolis. Contains polyphenolic compounds and flavonoids that function as antimicrobials. The purpose of this study was to analyze the inhibition zone formed due to the antimicrobial activity of propolis extract against the growth of Staphylococcus aureus and Eschericia coli bacteria. This research was conducted in vitro at the Microbiology Laboratory of the Medical Laboratory Technology Department of Health Poltekkes Surabaya in February – April 2021. This study used the Kirby Bauer diffusion method with paper blank discs on MHA media by measuring the diameter of the inhibition zone formed from the growth of Staphylococcus aureus and Eschericia coli with units of mm. Research The concentration of propolis extract used was 5%, 10%, 15%, and 20%. In this study, it was concluded that propolis extract can be used as an antimicrobial compound, there is a different effect of concentration of propolis extract on Staphylococcus aureus from Eschericia coli. Data analysis on the Kruskal Wallis test has a value of 0.004 (<0.05) which indicates that there is a significant difference between each concentration of propolis extract both against Staphylococcus aureus and Eschericia coli. The effect of the effective concentration is at a concentration of 20% against Staphylococcus aureus which has an average inhibition zone diameter of 13 mm while that of Eschericia coli is 22.5 mm.
Identification of eggs of soil transmitted helminthes (STH) intestinal nemathodes in children by flotation method in Tengket village, Arosbaya district, Bangkalan regency Dawami, Tamymatur Roudhatud; Pestariati, Pestariati; Arifin, Syamsul
Journal of Indonesian Medical Laboratory and Science Vol 5 No 1: April 2024
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i1.174

Abstract

Background: Worm infections can occur in humans throughout the world regardless of age and attack children's health. This statement encouraged researchers to do this in Tengket Village, where most guardians ignore their children's PBHS and latrines in rivers around residents' houses cause worm infections. More than 1.5billion people, or 24% of the world's population, are infected with soil-borne worms. That are transmitted through the soil are also called nematodes of the STH class. Objectives: The study aimed to identify STH intestinal nematode eggs in children in Tengket Village, Arosbaya, Bangkalan.  Materials and Methods:  The method used is flotation using NaCl reagent with the principle of different specific gravity so the parasites in the feces will be floated on the surface. The floatation method is able to produce a clean field of view and an easy procedure, thereby minimizing false positives and false negatives. Results: The research was carried out in February 2023 and the results obtained were that 9 out of 30 children's feces samples tested positive for worms. The positive results in the samples could be due to river latrines still being used and the spread occurring due to lack of attention to PHBS. Conclusions: Based on the research results, the percentage of worms has increased.
The effectiveness of using ID broth in identifying the outer membrane protein of Salmonella typhi Pestariati, Pestariati; Suhariyadi, Suhariyadi
International Journal of Public Health Science (IJPHS) Vol 14, No 1: March 2025
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v14i1.23912

Abstract

Typhoid fever is an infection that affects the digestive system. It spreads through contaminated food and drinks due to the Salmonella bacteria. One way to develop immunity against Salmonella typhi is by using outer membrane protein (OMP), which activates the cellular immune system. This research aimed to determine the effectiveness of using ID Broth to identify OMP Salmonella typhi. The study was conducted experimentally at the Institute of Tropical Disease from April 2023 to May 2023. For the study, we obtained three samples of Salmonella typhi isolated from East Java, and each sample was replicated three times. We isolated the bacteria and extracted the OMP to measure its levels and perform electrophoresis with SDS-PAGE. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a standard protein analysis method. To address sample loading challenges due to stacking gel transparency, an acidic dye was added to improve visibility without affecting gel performance. In this study nutrient agar from MacConkey medium and ID broth were used as variables. We cultured Salmonella typhi and extracted OMP using the sonication technique. We measured protein levels through the nanodrop method. Salmonella typhi from ID broth produced higher protein levels than Salmonella typhi cultured from MacConkey Medium. It affected the identification of OMP using SDS-PAGE. Lower protein levels lead to fewer protein molecules in the same band zone, causing reduced visibility and readability of the protein bands. The ID broth stabilizes the bacteria's condition before being grown on nutrient agar media.
RESISTANCE PATTERN OF Escherichia Coli AGAINTS ANTIBIOTICS IN URINARY TRACT INFECTION PATIENTS IN RSUD DR. SOETOMO SURABAYA Ariana, Novi; Pestariati, Pestariati; Sasongkowati, Retno; Kusumaningrum, Debby
Journal of Community Medicine and Public Health Research Vol. 1 No. 2 (2020): Journal of Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.827 KB) | DOI: 10.20473/jcmphr.v1i2.21693

Abstract

Pathogenic bacteria cause Urinary Infection imore or lessi about 85 - 95% are bacteria Escherichia coli. The treatment of Urinary Tract Infection (UTI) requires adequate supportive therapy and antibiotics, but antibiotic resistance is an obstacle in UTI treatment. This study aims to determine the types of antibiotics that are resistant to Escherichia coli in patients with UTI. This is an observational study with a descriptive method to determine the type of antibiotic-resistant to Escherichia coli in patients with UTI in the Laboratory of Clinical Microbiology RSUD Dr. Soetomo. Antibiotics that were resistant to Escherichia coli in UTI patients were cefazolin (80.49%), Amoxicillin Clavulanic Acid (73.17%), Ampicillin (73.17%), Tetracycline (68.29%), while antibiotics that were resistant to Escherichia coli ESBL was Amoxicillin Clavulanic Acid (100%), Ampicillin (100%), Cefazolin (99.7%), Aztreonam (99.7%), Ceftazidime (99.7%). The prevalence of Escherichia coli in UTI patients was 27.5%, while Escherichia coli ESBL was 72.5%. Based on the results of the research that has been obtained, it is better to do research on antibiotic resistance patterns periodically.
Identification Of Types Of Fungus Candida Albicans In The Urine Of Pregnant Women In The Thirty Trimester At RSI Surabaya Jemursari Iis Rofiatul Ana; Pestariati, Pestariati; Christ Kartika Rahayuningsih
Jurnal EduHealth Vol. 15 No. 04 (2024): Jurnal EduHealt (inpres), Year 2024
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Candidiasis is an infection caused by the fungus Candida albicans, especially in pregnant women in the third trimester. This infection, known as Vulvovaginal Candidiasis (VVC), occurs due to the growth of Candida on the vaginal wall. Increased estrogen hormone during pregnancy increases glycogen levels in the vagina, which is a source of carbon for Candida albicans. This study aims to identify the presence of Candida albicans in the urine of pregnant women in the third trimester at RSI Jemursari Surabaya. The study was descriptive qualitative involving 18 samples of pregnant women who experienced symptoms of itching in the vaginal area and vaginal discharge. The results showed that 5 pregnant women (27.8%) were positive for Candida albicans infection, while 13 pregnant women (72.2%) were negative. Examination was carried out macroscopically with Sabouraud Dextrose Agar (SDA) media, followed by microscopic examination using Lactophenol Cotton Blue (LPCB) staining, and a specific germ tube test. This study concluded that 27.8% of pregnant women in the third trimester were infected with Candida albicans from the identification results in urine samples.
Kolesterol dan Tekanan Darah Lansia di Posyandu Abiyoso Polkesbaya Christyaningsih, Juliana; Rusyadi, Luthfi; Minarti, Minarti; Mamik, Mamik; Pestariati, Pestariati; Mutiarawati, Diah Titik; Suliati, Suliati; Sasongkowati, Retno; Woelansari, Evy Diah; Suhariyadi, Suhariyadi; Istanto, Wisnu; Endarini, Lully Hanni; Museyaroh, Museyaroh
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 2 No 2 (2023): Jurnal Rakat Sehat: Pengabdian Kepada Masyarakat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v2i2.35

Abstract

As people age, the physical state of the human body will gradually deteriorate due to a decrease in the quantity of cells present, leading to a gradual reduction in functionality referred to as the aging process. In elderly humans (aged more than 60 years) excessive cholesterol levels and high blood pressure are one of the causes of diseases such as heart disease which has been recognized by WHO as the number one cause of death in the world. The buildup of fatty deposits on the linings of blood vessels has the potential to lead to obstructions within the blood vessels. The objective of this initiative is to enhance understanding regarding the hazards associated with cholesterol. This outreach effort took place in July 2023, conducted at Posyandu Abiyoso, Surabaya Health Polytechnic. The activity consists of several processes 1). conducting a survey of the location of the Abiyoso posyandu of the Surabaya Health Polytechnic, 2). Opening registration and taking participants' personal data, 3) conducting participant health interviews, 4). Checking cholesterol levels and blood pressure of participants. From the community service activities carried out, it was found that 42 of the 65 (64.6%) participants who took part in the examination at the integrated service post had high cholesterol levels, and 60 of the 65 (92.3%) participants had high blood pressure or hypertension. By conducting health checks on cholesterol levels and blood pressure, there is an expectation that the society will come to realize and comprehend. the dangers of hypertension and hypercholesterolemia so that the community can prevent the occurrence of sustainable diseases.
Studi Komparasi Hasil Pemeriksaan Mycobacterium Tuberculosis Pada Pasien Suspek Tuberkulosis Dengan Menggunakan Tes Cepat Molekuler dan Mikroskopis di RSUD Karangasem Wicaksana, I Nyoman Krisna; Pestariati, Pestariati; Arifin, Syamsul
Journals of Ners Community Vol 13 No 1 (2022): Journals of Ners Community
Publisher : Fakultas Ilmu Kesehatan Universitas Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55129/jnerscommunity.v13i1.1746

Abstract

Tuberkulosis merupakan suatu penyakit kronik menular yang disebabkan oleh Mycobacterium tuberculosis yang menyebabkan jumlah kematian global mencapai 1.3 juta pada tahun 2020 dan ditambah parah setelah adanya pandemi COVID-19 yang mengakibatkan terbatasnya layanan tuberkulosis, kasus di Indonesia selalu mengalami peningkatan tiap tahunnya, tahun 2019 ditemukan sebanyak 543.874 kasus meningkat dari 420.994 kasus di tahun 2017. Metode pemeriksaan yang banyak digunakan di negara endemik tuberkulosis adalah Mikroskopis, namun terdapat beberapa kekurangan dalam penggunaanya dibandingkan dengan metode Tes Cepat Molekuler. Penelitian ini bersifat observasional dengan pendekatan cross sectional yang bertujuan untuk mengetahui perbedaan hasil pemeriksaan Mycobacterium tuberculosis pada pasien suspek tuberkulosis dengan menggunakan Tes Cepat Molekuler dan Mikroskopis di RSUD Karangasem. Penelitian ini dilaksanakan dari bulan Desember 2021 sampai April 2022 menggunakan sampel sputum yang diperoleh dari RSUD Karangasem. Penelitian menunjukan adanya perbedaan hasil sensitivitas dan spesifisitas pada dua metode. Sensitivitas metode Tes Cepat Molekuler lebih tinggi daripada metode Mikroskopis (100% dan 75%) sedangkan nilai spesifisitas metode Mikroskopis lebih tinggi daripada metode Tes Cepat Molekuler (100% dan 95%).
Pemberdayaan Kader Kesehatan Dan Keluarga Pasien Tuberkulosis Dalam Pemanfaatan Sambiloto Sebagai Imunomodulator Untuk Keberhasilan Pengobatan Pasien Tuberkulosis Di Wilayah Kerja Puskesmas Gading Kecamatan Tambaksari Kota Surabaya Pestariati, Pestariati; Handayati, Anik; Suhariyadi, Suhariyadi
Sewagati Vol 9 No 3 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i3.2276

Abstract

Indonesia memiliki beban kasus tuberkulosis tertinggi ketiga di dunia. Tahun 2021, jumlah kasus tuberkulosis di Indonesia 824.000 kasus, hanya 402.502 kasus (49%) dari target 85% berhasil ditemukan dan diobati . Indikator keberhasilan pengob- atan di Jawa Timur tahun 2020 sebesar 87,1% (Target renstra Kemenkes 90%). Hal ini disebabkan salah satunya rendahnya pemahaman kader kesehatan, keluarga dan pasien tuberculosis tentang penyakitnya, termasuk bahaya resistensi OAT dan dukun- gan keluarga terhadap kesembuhan pasien. Sambiloto memiliki kandungan senyawa andrografolida, sebagai imunomodulator, meningkatkan keberhasilan pengobatan TB. Kader kesehatan berperan mengedukasi masyarakat tentang TB, memberikan dukungan dan motivasi kepada pasien TB,memfasilitasi kolaborasi antara pasien TB. Setiap Kelurahan Wilayah Puskesmas Gading memiliki Kader Kesehatan yang khusus menangani TB Pada Kelurahan di wilayah Puskesmas Gading, warga memi- liki tanaman Sambiloto baik dilakukan di Pot atau masuk di dalam TOGA RT. Pengabdian memberikan edukasi tentang tuberkulosis dan pemanfaatan sambiloto sebagai imunomodulator kepada kader kesehatan /TB dan keluarga pasien TB. Pen- ingkatan pemahaman kader dan keluarga pasien TB tentang sambiloto (N-Gain > 0,7) berhasil menurunkan angka BTA positif dari 84% menjadi 37,5%, sehingga edukasi (KIE) berkelanjutan sangat diperlukan.
Co-Authors Anindya, Lokawanti Al Fahna Ariana, Novi Christ Kartika Rahayuningsih Dawami, Tamymatur Roudhatud Endarini, Lully Hanni Evy Diah Woelansari Handayati, Anik Iis Rofiatul Ana Istanto, Wisnu Juliana Christyaningsih Kusumaningrum, Debby Luthfi Rusyadi Mamik Mamik Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Sasongkowati, Retno Suhariyadi Suhariyadi Suliati Suliati Syamsul Arifin Wicaksana, I Nyoman Krisna Wisnu, Istanto