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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Journal Of Vocational Health Studies Jurnal Media Analis Kesehatan Jurnal Ilmiah Tatengkorang Frontiers in Community Service and Empowerment Vitamin: Jurnal Ilmu Kesehatan Umum
Retno Sasongkowati
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Agriculture, Biological Sciences & Forestry Humanities Computer Science & IT Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Nursing Public Health Other

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EFEKTIVITAS EKSTRAK BIJI SIRSAK TERHADAP MORTALITAS Pediculus humanus var. Capitis SECARA IN VITRO Ahdiah Imroatul Muflihah; Retno Sasongkowati
Jurnal Media Analis Kesehatan Vol 12, No 1 (2021): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mak.v12i1.2002

Abstract

Pediculosis  capitis  adalah  gangguan  yang  disebabkan  oleh  infestasi  Pediculus humanus  capitis. Biji sirsak dapat berpotensi sebagai pedikulisida. Kandungan senyawa aktif dalam biji sirsak ini adalah Acetogenin, Annonain ,Flavonoid, dan Squamosin. Eksperimen ini bertujuan untuk mengetahui efektivitas ekstrak biji sirsak sebagai pedikulisida. Penelitian ini bersifat eksperimental yang dilakukan pada bulan maret-juni 2017 di Laboratorium Parasitology Poltekkes Kemenkes Surabaya. Penelitian ini menggunakan sampel berupa kutu kepala sebanyak 160 ekor dengan masing masing kelompok 5 ekor kutu kepala yang diberikan perlakuan dengan menyemprotkan ekstrak biji sirsak dengan konsentrasi 10%, 20%, 40%, 60%, 80%, dan 100%. Hasil penelitian ini menunjukkan bahwa konsentrasi paling efektif ialah 100% dalam waktu 10 menit, dan konsentrasi minimum adalah 10% dan 20% dalam waktu 50 menit.
Deteksi Jamur Candida albicans Pada Urine Penderita Infeksi Saluran Kemih Menggunakan Metode RT-PCR Retno Sasongkowati; Anita Dwi Anggraini; Dellanis Arina Putri
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.14409

Abstract

                                                        ABSTRAKCandida albicans merupakan anggota flora normal yang terdapat pada selaput mukosa,  saluran pencernaan, saluran pernafasan, uretra, vagina, kulit dan kuku tangan dan kaki. Candida albicans dapat tumbuh pada berbagai macam pH, tetapi umumnya pertumbuhan akan lebih baik pada pH antara 4,5-6,5. Candida albicans dapat tumbuh dalam perbenihan pada suhu 28ºC – 37ºC. Jenis penelitian yang digunakan merupakan penelitian deskriptif kuantitatif. Penelitian ini bertujuan untuk untuk mengetahui adanya jamur Candida albicans pada urine penderita infeksi saluran kemih menggunakan metode Real Time PCR. Populasi dalam penelitian penderita infeksi saluran kemih. Infeksi saluran kemih merupakan infeksi keadaan klinis akibat berkembangbiaknya mikroorganisme yang menyebabkan inflamasi pada saluran kemih. Penelitian dilaksanakan di Laboratorium Parasitologi dan Laboratorium Biologi Molekuler pada bulan April 2022 hingga Mei 2022. Pada penelitian ini dilakukan beberapa tahapan pemeriksaan seperti isolasi kultur pada media Sabaroud Dextrose Agar, pemeriksaan makroskopis dan mikroskopis, pembuatan suspensi sel, uji ekstraksi, uji kemurnian DNA, optimasi dan pemeriksaan Real Time PCR dengan menunjukkan hasil berupa CT. Hasil penelitian menunjukkan pada pemeriksaan Real Time PCR ini didapatkan sejumlah 2 sampel dengan persentase 22,2% pada kode sampel S7 dengan nilai CT 28,50 dan kode sampel S9 dengan nilai CT 23,19 dan hasil negatif sejumlah 7 sampel dengan persentase 77,8%. Adapun faktor penyebab yang dapat mengakibatkan keberadaan jamur Candida albicans pada urine penderita infeksi saluran kemih seperti personal hygiene, penderita diabetes mellitus, alat kontrasepsi dan penggunaan antiseptik. 
Analisa Keberadaan Jamur Trichophyton rubrum pada Kuku Kaki Petugas Kebersihan Dinas Lingkungan Hidup Surabaya Mitra Putri Mandiri; Retno Sasongkowati; Anita Dwi Anggraini
Vitamin : Jurnal ilmu Kesehatan Umum Vol. 2 No. 4 (2024): Oktober : Jurnal ilmu Kesehatan Umum
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/vitamin.v2i4.771

Abstract

Sanitation workers play a vital role in maintaining environmental cleanliness. However, many of them neglect their personal hygiene. Sanitation workers are professions that regularly wear closed shoes for long periods,the humid, hot, and dirty work environment promotes fungal infections in toenails, known as onychomycosis. The purpose of this study is to analyze the presence of the fungus Trichophyton rubrum on the toenails of the sanitation workers of the Surabaya Environmental Service Department. This study is a descriptive laboratory observation. The research was conducted from February 23 to March 13, 2024. Sample collection took place at Surabaya Environmental Service Department, and the study carried out at Mycology Laboratory of the Medical Laboratory Technology Department, Poltekkes Kemenkes Surabaya. The samples in this study consisted of toenails from 30 sanitation workers members. The examination was conducted using the culture method on SDA media, incubated for 7-14 days, followed by macroscopic and microscopic observations. The results showed that 2 samples were positive for Trichophyton rubrum (6.7%), 23 samples were positive for Aspergillus sp., (76.7%), 4 samples were positive for Rhizopus sp., (13.3%), and 1 sample was positive for Penicillium sp., (3.3%). The conclusion is, out of 30 samples, 2 individuals (6.7%) tested positive for Trichophyton rubrum, while the remaining 28 samples (93.3%) tested negative for Trichophyton rubrum.
DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS Prisma Anjarlena; Suliati; Wisnu Istanto; Retno Sasongkowati
Journal of Vocational Health Studies Vol. 7 No. 1 (2023): July 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V7.I1.2023.17-23

Abstract

Background: Diabetes Mellitus (DM) patients are susceptible to wound infections brought on by S. aureus bacteria, in dealing with infections caused by bacteria, antibiotic treatment was conducted. Nevertheless, misapplication of antibiotics might lead to drug resistance or MRSA. Panton-Valentine Leukocidin (PVL) is a crucial factor of S. aureus pathogenicity. Skin and soft tissue infections can be caused by the PVL gene. Purpose: To detect PVL gene in ulcer swabs of diabetic mellitus patients against MRSA strains. Method: Employing descriptive quantitative data analysis of PVL gene detection observations on ulcer swab of diabetes mellitus patients using the RT-PCR method. Ulcer swab samples were collected at Dharmahusada and Banyu Urip Diabetes Wound Specialist Hospital, Surabaya, East Java, Indonesia. The research was conducted at the Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Bacteriology Laboratory for the isolation and identification of MRSA and Molecular Biology Laboratory for the detection of the PVL gene in April–May 2022. Result: From 30 samples of ulcer swabs, 8 samples were positive for MRSA and 22 samples were negative for MRSA. After that, the MRSA positive samples were detected using the RT-PCR method to detect the PVL gene. The results of PVL detection were obtained from 6 samples (75%) positive for detecting the PVL gene and 2 samples (25%) negative for detecting the PVL gene. Conclusion: The results of the study showed that ulcer swabs from diabetes mellitus patients had the PVL gene against MRSA.
Blood Glucose Testing on Blood Donors and Geriatrics in the Area of Mosque Al-Akbar Surabaya museyaroh, museyaroh; Juliana Christyaningsih; Retno Sasongkowati; Suliati; Anita Dwi Anggraini; Deddy Adam; Toni Watoyani
Frontiers in Community Service and Empowerment Vol. 4 No. 2 (2025): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i2.98

Abstract

The Indonesian Red Cross (IRC) is a humanitarian organization that plays an important role in providing assistance to the community, particularly in the fields of health, disaster response, and blood donation services. The volunteer team from Health Polytechnic, Ministry of Health, Surabaya, in collaboration with members of the Indonesian Association of Medical Laboratory Technologists Surabaya city and IRC Surabaya city, conducted free blood glucose level tests for prospective donors and the elderly (geriatric) in Al-Akbar Mosque Surabaya. This examination aims to detect potential health issues early, especially diabetes, which often poses challenges for blood donors and the elderly. Blood donors tend to neglect their health conditions due to the donation routine, so this examination ensures that they remain in optimal condition. Meanwhile, the elderly have a high risk of diabetes, which can affect their quality of life. The strategic location at Al-Akbar Mosque Surabaya facilitates access for the community who are far from the IRC Surabaya city office. The success of this activity is supported by various factors, including the involvement of professional health organizations such as the branch Surabaya City of Indonesian Association of Medical Laboratory Technologists, the support of health infrastructure from IRC Surabaya city, and the active participation of the community and volunteers. The respondents of the community service activity numbered 88 people, conducted on March 15 and 17, 2025, consisting of 59% men and 41% women, with an age range of 20-80 years and the majority of respondents aged 41-60 years (56.8%). From the examination results, it was found that 21% of male respondents and 13.9% of female respondents suffer from diabetes mellitus (DM), thus requiring medical follow-up and lifestyle changes. This activity is expected to raise public awareness about the importance of early diabetes detection and encourage a sustainable healthy lifestyle to prevent serious complications in the future
AKSI KOMPETENSI BERSAMA DI PANTI ASUHAN MISI NUSANTARA SURAKARTA Juliana Christyaningsih; Puspitadewi, Teresia Retna; Alberta, Lembunai Tat; Pengge, Nuning Marina; Siagian, Hotmaida; Mutiarawati, Diah Titik; Rahayuningsih, Christ Kartika; Kurniawan, Dedi; Lestari, Indah; Sumasto, Hery; Endarini, Lully Hani; Kiaonarni OW; Ira Puspita Sari; Evi Yunita N; Suliati; Darjati; Nur Hatijah; Khambali; Hadi Suryono; Demes Nurmayanti; Tatarini Ika Pipitcahyani; Astuti Setiyani; Siti Alfiah; Dina Isfentiani; Bambang Hadi Sugito; Retno Sasongkowati; Yusianti Silviani; N. S. Widodo
Jurnal Ilmiah Tatengkorang Vol 7 No 1 (2023): Jurnal Ilmia Tatengkorang
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Politeknik Negeri Nusa Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54484/tkrg.v7i1.553

Abstract

Permasalahan sosial yang banyak terjadi di masyarakat adalah kaum marjinal yang terpinggirkan seperti anak terlantar, anak jalanan, pengemis, dan sebagainya. Salah satu upaya mengatasi masalah kaum marjinal yaitu dengan didirikan lembaga sosial berupa panti asuhan anak. Sejak masa pandemi, anak di Panti Asuhan Misi Nusantara Surakarta jarang mendapat layanan kesehatan dan ada keterbatasan ruang gerak anak-anak. Tujuan pengabdian masyarakat ini untuk membantu panti asuhan dalam memantau pertumbuhan dan perkembangan penghuni dan pengelola Panti Asuhan Misi Nusantara di Surakarta. Metoda pelaksanaan berupa pemeriksaan tumbuh kembang anak, Penyuluhan Hidup Bersih dan Sehat, pemeriksaan Status Gizi Anak, penyuluhan cara menyikat gigi yang benar, pemeriksaan kesehatan pengelola panti asuhan serta pelatihan penanggulangan bencana. Hasil pemeriksaan Kesehatan pada penghuni dan pengelola panti didapatkan sebagian besar (52,6 persen) penghuni panti berjenis kelamin laki-laki, hampir setengahnya berusia rata-rata 21-60 tahun, Sebagian besar (68,4 persen) tekanan darah kurang dari 140/90 mmHg, Sebagian besar (73,1 persen) berstatus gizi normal, hampir seluruhnya (94,4 persen) glukosa darah kurang dari 126 m/dL dan asam urat kurang dari 8 mg/dL. Secara umum keadaan warga panti asuhan dalam keadaan baik Social problems that often occur in society are abandoned children, street children, beggars, disabled people, neglected elderly, poor families, families with social problems, and so on. Children and poor people should have their human rights fulfilled by the State of Indonesia. However, in reality there are many children whose needs are not met, such as parents who are unable to provide for the child's needs or the child does not have parents. One effort to overcome this is to establish social institutions in the form of orphanages. Since the pandemic, children at the Mission Nusantara Surakarta Orphanage rarely receive health services and there is limited space for children to move. Theless than purpose of this community service is to assist the orphanage in monitoring the growth and development of residents and managers of the Mission Nusantara Orphanage in Surakarta. The implementation method is in the form of examinations on child growth and development, Counseling on Clean and Healthy Living, checking on the Nutritional Status of Children, counseling on how to brush their teeth properly, health checks for orphanage managers and training on disaster management. 6 percent) of the residents of the orphanage are male, almost half of them are aged 21-60 years, most (68.4 percent) have blood pressure less than 140/90 mmHg, most (73.1 percent) have normal nutritional status, almost all (94.4 percent) blood glucose less than 126 m/dL and uric acid less than 8 mg/dl. Similar activities are needed to see general body health and must be monitored periodically, so that the residents of the orphanage can find out their health status, especially now that they are still in a pandemic condition Covid-19
Co-Authors Ahdiah Imroatul Muflihah Anita Dwi Anggraini Astuti Setiyani Bambang Hadi Sugito Darjati Deddy Adam Dedi Kurniawan Dellanis Arina Putri Demes Nurmayanti Endarini, Lully Hani Evi Yunita N Hadi Suryono Indah Lestari, Indah Ira Puspita Sari Isfentiani, Dina Juliana Christyaningsih Khambali, Khambali Kiaonarni OW Lembunai Tat Alberta, Lembunai Tat Mitra Putri Mandiri Museyaroh, Museyaroh Mutiarawati, Diah Titik N. S. Widodo Nur Hatijah Pengge, Nuning Marina Prisma Anjarlena Puspitadewi, Teresia Retna Rahayuningsih, Christ Kartika Siagian, Hotmaida Siti Alfiah Suliati Sumasto, Hery Toni Watoyani Wisnu Istanto Yusianti Silviani