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PERBEDAAN KADAR KOLESTEROL HDL DAN KADAR KOLESTEROL TOTAL PADA PEROKOK AKTIF DENGAN PEROKOK PASIF YANG MENDERITA DIABETES MELITUS DI PUSKESMAS TANJUNGANOM KABUPATEN NGANJUK Sadgassusi Hera Samudra; Edy Haryanto; Syamsul Arifin; Wisnu Istanto
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/anakes.v10i1.9

Abstract

ABSTRACTCigarettes contain various harmful substances that can endanger health. One of them is nicotine which causeslipid metabolism disorders, including a decrease in HDL cholesterol levels and an increase in total cholesterollevels. Smoking habits in people with comorbidities such as diabetes mellitus can exacerbate existingdyslipidemia conditions. If not managed properly, it can cause complications that lead to increased mortality inpeople with diabetes mellitus. This study aims to determine the differences in HDL cholesterol and totalcholesterol levels in active smokers and passive smokers patients with diabetes mellitus at PuskesmasTanjunganom, Nganjuk Regency. This research uses observational study with a cross sectional approach whichwas conducted in January-May 2021 on 30 patients with diabetes mellitus at Puskesmas Tanjunganom, NganjukRegency, consisting of 15 active smokers and 15 passive smokers. Checking total cholesterol levels was carriedout at Puskesmas Tanjunganom, Nganjuk Regency. While the examination of HDL cholesterol levels wascarried out at UPT Labkesda, Nganjuk Regency. Based on the independent sample t-test statistical test, it wasfound that the p-value of difference HDL cholesterol levels in active smokers and passive smokers with diabetesmellitus was 0,000 and the difference total cholesterol levels in active smokers and passive smokers withdiabetes mellitus was 0.012. So it can be concluded that statistically there are differences in HDL cholesteroland total cholesterol levels in active smokers and passive smokers who suffer from diabetes mellitus atPuskesmas Tanjunganom, Nganjuk Regency.Keywords: Diabetes Mellitus; Cholesterol Levels; Active Smoker; Passive SmokerABSTRAK Rokok mengandung berbagai zat berbahaya yang dapat menggangu kesehatan. Salah satunya nikotin yangmengakibatkan gangguan metabolisme lipid antara lain penurunan kadar kolesterol HDL dan peningkatan kadarkolesterol total. Kebiasaan merokok pada orang dengan penyakit penyerta seperti diabetes melitus dapatmemperburuk kondisi dislipidemia yang sudah ada. Apabila tidak dikelola dengan baik dapat mengakibatkankomplikasi yang menyebabkan peningkatan mortalitas penderita diabetes melitus. Penelitian ini bertujuan untukmengetahui perbedaan kadar kolesterol HDL dan kolesterol total pada perokok aktif dengan perokok pasif yangmenderita diabetes melitus di Puskesmas Tanjunganom Kabupaten Nganjuk. Penelitian ini merupakan penelitanobservasional dengan pendekatan cross sectional yang dilakukan pada bulan Januari-Mei 2021 terhadap 30pasien penderita diabetes melitus di Puskesmas Tanjunganom Kabupaten Nganjuk yang terdiri atas 15 orangperokok aktif dan 15 orang perokok pasif. Pemeriksaan kadar kolesterol total dilakukan di LaboratoriumPuskesmas Tanjunganom Kabupaten Nganjuk. Sedangkan pemeriksaan kadar kolesterol HDL dilakukan di UPTLabkesda Kabupaten Nganjuk. Berdasarkan uji statistik independent sampel t-test diperoleh p-value perbedaankadar kolesterol HDL pada perokok aktif dengan perokok pasif penderita diabetes melitus sebesar 0,000 sertaperbedaan kadar kolesterol total pada perokok aktif dengan perokok pasif penderita diabetes melitus sebesar0,012. Sehingga dapat disimpulkan bahwa secara statistik terdapat perbedaan kadar kolesterol HDL dankolesterol total pada perokok aktif dengan perokok pasif yang menderita diabetes melitus di PuskesmasTanjunganom Kabupaten Nganjuk.Kata Kunci : diabetes melitus; kadar kolesterol; perokok aktif; perokok pasif
Peningkatan Kemandirian Siswa dalam Menerapkan Pola Hidup Bersih dan Sehat untuk Mencegah dan Mengurangi Infeksi Soil Transmitted Helminths (Sth) di SD MI Sunan Ampel 1 Sidorogo-Trosobo Kecamatan Taman Kabupaten Sidoarjo Suliati; Wisnu Istanto; Anita Dwi Anggraini
ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN
Publisher : Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/abdikesmasmulawarman.v3i1.156

Abstract

Soil Transmitted Helminths (STH) adalah golongan cacing usus (Nematoda Usus) dalam perkembanganya membutuhkan tanah untuk men jadi bentuk infektif. Golongan STH yang habitatnya pada usus manusia adalah Ascaris lumbricoides, Hookworm (Necator americanus dan Ancylostoma duodenale), Strongiloides stercoralis, Trichuris trichiura. Madrasah Ibtidaiyah Sunan Ampel II mulai kiprahnya dalam dunia pendidikan sejak tahun1988 atas prakarsa beberapa tokoh agama, tokoh masyar akat dan adanya tuntutan dari masyarakat setempat. Mengingat semakin banyaknya output yang dihasilkan. Madrasah Ibtidaiyah Sunan Ampel II setiap tahunnya, juga kondisi masyarakat setempat yang tergolong mene ngah kebawah, sedangkan letak desa tersebut jauh dari lembaga pendidikan SMP dan setingkatnya yang ada disekitar desa tersebut. Penelitian dilakukan oleh Megadkk tahun 2020 di SD MI Sunan Ampel 1 Desa Sidorogo Kecamatan Taman Kabupaten di didapatkan hasil terdapat siswa sebanyak 44% terinfeksi STH, hal ini dikarenakan siswa yang kurang pengetahuan tentang kecacingan sebanyak 19 siswa (76%), sedangkan cukupnya pengetahuan tentang kecacingan sebanyak 4 siswa (16%), dan baiknya pengetahuan tentang kecacingan sebanyak 2 siswa (8%). dengan jumlah keseluruhan sebanyak 25 anak (100%). sebagian besar siswa maupun orang tua belum mengenal jenis-jenis cacing yang berbahaya bagi kesehatan, ataupun mengetahui cara pencegahan dan akibat kecacingan. Upaya yang dapat dilakukan adalah kegiatan penyuluhan yang berlangsu ng sangat interaktif dan komunikatif dan setiap peserta penyuluhan dapat memahami penjelasan yang disampaikan baik melalui media poster baik melalui tanya jawab. Berdasarkan hasil pretest dan post terdapat peningkatan pengetahun rata-rata 23% menjadi 71% ,sehingga total peningkatan pengetahuan peserta bertambah sebesar 48%.
The Approval of Reading Speed Capacities for Laboring Textbooks to Improve Reading Speed for Laboring Textbooks for Students of Vocational Health Era Yunita Fitria; Wisnu Istanto; Noer Amelia; Merlissa Suemith
Bulletin of Science Education Vol. 4 No. 2 (2024): Bulletin of Science Education
Publisher : CV. Creative Tugu Pena

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51278/bse.v4i2.1431

Abstract

If students are to pursue vocational health or any other technical area of specialization, then they have to acquire effective reading skills. Most learners experience difficulties in speed and comprehension, which impacts negatively on both academic and professional pursuits later in life. This problem is exacerbated by the technical nature of the language used in vocational health education and the bulk of information carried within textbooks, which is imperative in translating classroom knowledge into practice. This study aims to improve the reading speed of labor-intensive textbooks that vocational health students go through, as a professional would face situations in the field where interpreting data speedily is inevitable. While reading speed and comprehension remain critical, remarkably few published studies have covered this topic in vocational health. The present study fills that gap by examining some interventions for improving reading speed for the type of learner under study. This present study employed a mixed-methods approach, which involved gathering both qualitative and quantitative data. For the current study, the participants included 78 vocational health students. An experimental group received targeted speed-reading and comprehension strategies, while the control group followed their normal study routine. Descriptive statistics, T-tests, and correlation analyses were applied to analyze pre-test and post-test data on reading speed and comprehension, together with students' feedback. While the control group indicated very slight improvements, the experimental group showed significant gains in reading speed and comprehension, thus targeted interventions could work well.
DETECTION OF PANTON-VALENTINE LEUKOCIDIN (PVL) GENE AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN DIABETIC ULCER PATIENTS Prisma Anjarlena; Suliati; Wisnu Istanto; Retno Sasongkowati
Journal of Vocational Health Studies Vol. 7 No. 1 (2023): July 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V7.I1.2023.17-23

Abstract

Background: Diabetes Mellitus (DM) patients are susceptible to wound infections brought on by S. aureus bacteria, in dealing with infections caused by bacteria, antibiotic treatment was conducted. Nevertheless, misapplication of antibiotics might lead to drug resistance or MRSA. Panton-Valentine Leukocidin (PVL) is a crucial factor of S. aureus pathogenicity. Skin and soft tissue infections can be caused by the PVL gene. Purpose: To detect PVL gene in ulcer swabs of diabetic mellitus patients against MRSA strains. Method: Employing descriptive quantitative data analysis of PVL gene detection observations on ulcer swab of diabetes mellitus patients using the RT-PCR method. Ulcer swab samples were collected at Dharmahusada and Banyu Urip Diabetes Wound Specialist Hospital, Surabaya, East Java, Indonesia. The research was conducted at the Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Bacteriology Laboratory for the isolation and identification of MRSA and Molecular Biology Laboratory for the detection of the PVL gene in April–May 2022. Result: From 30 samples of ulcer swabs, 8 samples were positive for MRSA and 22 samples were negative for MRSA. After that, the MRSA positive samples were detected using the RT-PCR method to detect the PVL gene. The results of PVL detection were obtained from 6 samples (75%) positive for detecting the PVL gene and 2 samples (25%) negative for detecting the PVL gene. Conclusion: The results of the study showed that ulcer swabs from diabetes mellitus patients had the PVL gene against MRSA.
Deteksi Gen blaTEM Dari Bakteri Klebsiella Pneumoniae Penghasil Extended Spectrum Beta-Lactamase (ESBL) Pada Pasien Infeksi Saluran Kemih Widyawati, Keri Ayu; Suliati; Anggraini, Anita Dwi; Wisnu Istanto
Jurnal Media Analis Kesehatan Vol 15 No 2 (2024): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v15i2.814

Abstract

Infeksi saluran kemih merupakan infeksi yang terjadi pada saluran uretra dan kandung kemih yang disebabkan oleh bakteri Klebsiella pneumoniae salah satunya. Pengobatan atau terapi yang sering digunakan untuk penderita infeksi saluran kemih yakni dengan pemberian antibiotik golongan beta-laktam. Namun, terdapat beberapa bakteri yang memiliki enzim beta-laktamase yang merupakan salah satu penyebab bakteri resisten terhadap antibiotik. Enzim tersebut dikodekan oleh gen salah satunya gen TEM yang dapat menghidrolisis antibiotik golongan beta-laktam. Tujuan studi ini untuk mendeteksi gen blaTEM terhadap bakteri Klebsiella pneumoniae penghasil Extended Spectrum Beta-Lactamase (ESBL) yang diisolasi dari urin pasien infeksi saluran kemih. Metode penelitian memanfaatkan Deskriptif kuantitatif dengan jumlah sampel penelitian 30 pasien infeksi saluran kemih di PSPAL Dr, Ramelan Surabaya. Studi ini dilakukan pada bulan Februari – Mei 2024. Identifikasi dan uji sensitivitas antibiotik memanfaatkan alat Vitek 2 Compact, uji konfirmasi ESBL menggunakan metode DDST, dan deteksi gen blaTEM menggunakan alat PCR Konvensional. Hasil penelitian menunjukkan bahwa terdapat 7(23%) sampel yang positif gen blaTEM, ditandai dengan terbentuknya pita DNA dengan panjang basa 445bp pada elektroforesis. Dapat disimpulkan bahwa gen blaTem pada bakteri Klebsiella pneumoniae terdeteksi sebagai produsen ESBL pada pasien infeksi saluran kemih
PERILAKU SEHAT DAN BERSIH PENGHUNI PANTI YAYASAN CINTA KASIH IBU TERESA SURABAYA Pratiwi Hermiyanti; Lembunai Tat Alberta; Darjati; Wisnu Istanto; Mujayanto; Khambali; Demes Nurmayanti; Hadi Suryono; Fitri Rokhmalia; Rachmaniyah; Iva Rustanti; Marlik; Putri Arida Ipmawati; Budi Yulianto
Jurnal Ilmiah Tatengkorang Vol 7 No 2 (2023): Jurnal Ilmiah Tatengkorang
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Politeknik Negeri Nusa Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54484/tkrg.v7i2.585

Abstract

Panti Yayasan Cinta Kasih (YCK) Ibu Teresa adalah salah satu panti berlokasi di Surabaya Barat yang merawat anak dan bayi serta orang tua lanjut usia yang membutuhkan perawatan dan pendidikan keluarga. Bantuan dari masyarakat sekitar terkait pangan sudah memenuhi kebutuhan di Panti YCK Ibu Teresa, namun pendidikan dasar kesehatan belum sepenuhnya diperoleh secara berkala bagi warga panti ini. Tujuan pengabdian masyarakat ini untuk memberikan pengetahuan serta perilaku yang mendukung pendidikan dasar kesehatan pada anak dan orang tua lanjut usia. Metode pelaksanaan yang dilakukan berupa penyuluhan Perilaku Hidup Bersih dan Sehat (PHBS) serta penyuluhan cara menyikat gigi yang benar. Hasil pengabdian masyarakat penyuluhan Perilaku Hidup Bersih dan Sehat dan cara sikat gigi yang benar diikuti secara menyeluruh oleh warga YCK Ibu Teresa dan antusias dalam memberikan pertanyaan terkait materi yang diberikan. Diharapkan dalam waktu periodik dilakukan pengabdian masyarakat sejenis serta pelayanan kesehatan lainnya untuk merawat kesehatan warga YCK Ibu Teresa Surabaya. Yayasan Cinta Kasih (YCK) Ibu Teresa Orphanage is one of the orphanages located in West Surabaya that cares for children and babies as well as elderly parents who need family care and education. Assistance from the surrounding community regarding food has met the needs of the YCK Ibu Teresa Orphanage, but basic health education has not been yet fully provided on a regular basis for the residents of this orphanage. The purpose of this community service is to provide knowledge and behavior that supports basic health education for children and elderly parents. The implementation method used is in the form of counseling on Clean and Healthy Behavior (PHBS) as well as counseling on how to brush teeth properly. The results of the community service counseling on Clean and Healthy Behavior and the correct way to brush teeth were followed thoroughly by YCK Ibu Teresa residents, and enthusiastically ask questions related to the material presented. It is hoped that in the periodic time similar community service and other health services will be carried out to care health of YCK Ibu Teresa residents, Surabaya
Korelasi Kadar Serum Iron Dengan Hemoglobin Pada Pasien Gagal Ginjal Kronik Level V Di RSUD Dr. Harjono S Ponorogo Yulia Hermawati; Dwi Krihariyani; Syamsul Arifin; Wisnu Istanto
JOURNAL SAINS STUDENT RESEARCH Vol. 3 No. 4 (2025): Jurnal Sains Student Research (JSSR) Agustus
Publisher : CV. KAMPUS AKADEMIK PUBLISING

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61722/jssr.v3i4.5975

Abstract

Chronic Kidney Disease (CKD) is not merely a condition of permanently decreased renal function, but it also significantly impacts patients’ quality of life. One of the major complications is anemia. Iron deficiency is a primary contributor to anemia in CKD patients, often resulting from reduced erythropoietin production, blood loss during hemodialysis, and inadequate iron intake. This study aims to determine the relationship between serum iron levels (iron in the blood) and hemoglobin levels in stage V chronic kidney disease (CKD) patients undergoing regular hemodialysis at Dr. Harjono S. Ponorogo Regional Hospital, using a quantitative descriptive-analytic approach based on secondary data from the hospital's Laboratory Information System (LIS). A total of 24 CKD patients undergoing hemodialysis twice a week during the period of July to December 2024 were included as research subjects. The results showed that all patients experienced anemia. Although most patients had serum iron levels within the normal range, hemoglobin levels remained low. Statistical analysis revealed a moderate correlation between serum iron and hemoglobin levels, with a statistically significant p-value (p < 0.05) in both examinations. A moderate and statistically significant correlation was found between serum iron and hemoglobin levels in stage V CKD patients, indicating that monitoring iron status is important in the management of anemia in chronic kidney disease.