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All Journal Media Kedokteran Hewan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Dental Journal (Majalah Kedokteran Gigi) Jurnal Medik Veteriner JURNAL PENDIDIKAN TAMBUSAI Journal of Parasite Science journal of Basic Medical Veterinary Berkala Penelitian Hayati Jurnal Kedokteran Hewan Jurnal Agro Veteriner
Erma Safitri
Department Of Veterinary Reproduction - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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The role of heat shock protein (HSP ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture Sri Wigati Mardi Mulyani; Ernie Maduratna Setiawati; Erma Safitri; Eha Renwi Astuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 1 (2014): March 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.11 KB) | DOI: 10.20473/j.djmkg.v47.i1.p41-44

Abstract

Background: The concept of stem cell therapy is one of the new hope as a medical therapy on salivary gland defect. However, the lack of viability of the transplanted stem cells survival rate led to the decrease of effectiveness of stem cell therapy. The underlying assumption in the decrease of viability and function of stem cells is an increase of apoptosis incidence. It suggests that the microenvironment in the area of damaged tissues is not conducive to support stem cell viability. One of the microenvironment is the hypoxia condition. Several scientific journals revealed that the administration of hypoxic cell culture can result in stress cells but on the other hand the stress condition of the cells also stimulates heat shock protein 27 (HSP 27) as antiapoptosis through inhibition of caspase 9. Purpose: The purpose of this study was to examine the role of heat shock protein 27 as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture. Methods: Stem cell culture was performed in hypoxic conditions (O2 1%) and measured the resistance to apoptosis through HSP 27 and caspase 9 expression of bone marrow mesenchymal stem cells by using immunoflorecence and real time PCR. Results: The result of study showed that preconditioning hypoxia could inhibit apoptosis through increasing HSP 27 and decreasing level of caspase 9. Conclusion: The study suggested that hypoxic precondition could reduce apoptosis by increasing amount of heat shock protein 27 and decreasing caspase 9.Latar belakang: Konsep terapi stem cell merupakan salah satu harapan baru sebagai terapi medis kelainan kelenjar ludah. Namun, rendahnya viabilitas stem cell yang ditransplantasikan menyebabkan penurunan efektivitas terapi. Asumsi yang mendasari rendahnya viabilitas dan fungsi stem cell adalah tingginya kejadian apoptosis. Hal ini menunjukkan bahwa lingkungan mikro di daerah jaringan yang rusak tidak kondusif untuk mendukung viabilitas stem cell. Salah satu lingkungan mikro adalah kondisi hipoksia. Beberapa jurnal ilmiah mengungkapkan bahwa kondisi hipoksia pada kultur sel dapat menyebabkan sel-sel stres, namun di sisi lain kondisi stres sel juga merangsang heat shock protein 27 (HSP 27) sebagai antiapoptosis dengan menghambat ekspresi caspase 9. Tujuan: Tujuan penelitian ini adalah untuk meneliti peran protein heat shock 27 sebagai inhibitor apoptosis dalam kondisi hipoksia kultur stem cell sumsum tulang. Metode: Kultur stem sel dilakukan dalam kondisi hipoksia (O2 1%) dan mengukur resistensi terhadap apoptosis melalui ekspresi HSP 27 dan caspase 9 stem cell mesenchymal sumsum tulang dengan menggunakan immunoflorecence dan PCR real time. Hasil: Hasil penelitian menunjukkan bahwa prakondisi hipoksia dapat menghambat apoptosis melalui peningkatan HSP 27 dan penurunan tingkat Caspase 9. Simpulan: Studi ini menunjukkan bahwa prakondisi hipoksia dapat mengurangi apoptosis dengan meningkatkan jumlah protein heat shock 27 dan penurunan caspase 9.
ANTI-PROLAKTIN SEBAGAI PENGHAMBAT PROSES MOULTING Husni Anwar; Erma Safitri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/455

Abstract

A experimental study had been done using anti-prolactin as moulting process inhibitor and toknow the affect of anti-prolactin on the time of egg laying recovery of laying bird. The objective of this study was to prove that, moulting phase can be stopped with pasif imunisation antiprolactin intra muscular. The stages of this study as follow: used anti-prolactin from the third stage of study with the dose of 50 mg/ml (P1), 100 mg/ml (P2), and 200 mg/ml (P3) and 0.5 ml PBS (as control). In this stage, moulting was ceased respectively in day 4.8 ± 1.033 (P1); 4.6 ± 0.843 (P2); 4.68 ± 0.516 (P3) and 61.9 ± 2.079 (control). Statistical test using one way Anova revealed significant difference (p < 0.01) between control and treatment groups, and BNT 5% test showed that the fastest moulting inhibition was found in P3 group (200 mg/ml), which was not significantly different (p < 0.05) with P1 (50 mg/ml) and P2 (100 mg/ml). The time of egg laying recovery was respectively at 9.3 ± 0.675 (PI); 7.4 ± 0.843 (P2); 3.3 ± 0.823 (P3) and 18.4 ± 1.174 (control). Statistical test using one way Anova test showed significant difference (p < 0.01) between control and treatment groups, and BNT 5% test indicated that the shortest time of egg laying recovery was at group P3 (200 mg/ml), which was significantly different (p < 0.05) from Pl (50 mg/ml); P2 (100 mg/ml); and control (PBS 0.5 ml). Conclusively, anti prolactin (50 mg/ml, 100 mg/ml and 200 mg/ml) to inhibit moulting process and to affect the time of egg laying recovery.
METODE PEMBUATAN ANTIPROLAKTIN PADA HEWAN COBA KAMBING LOKAL SEBAGAI PENGHAMBAT PROSES RONTOK BULU PADA AYAM ARAB PETELUR Erma Safitri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/458

Abstract

Anti-prolactin has a specific activity against prolactin. It neutralizes prolactin action in circulation. The effect of such neutralization is the inhibition of feather fall off process, so that hens may be able to produce eggs again. Anti-prolactin can be produced by injecting prolactin isolate from blood serum of arabic hens in feather fall off-phase into local goat. Prolactin isolate was immunized to local goat to produce anti-prolactin. Six local goats were divided into 2 groups. The first group comprised 1 goat immunized with PBS, and the second one was immunized with prolactin isolate in CFA and subjected to booster with prolactin isolate in IFA twice. The formation of anti-prolactin and the highest titer was detected using indirect ELISA. The results of this study showed that (1) Anti-prolactin could be produced in goat from the prolactin isolate of feather fall off-phase arabic hens blood serum; (2). The first emergence of Antiprolactin was at the first bleeding after immunization of prolactin isolate in CFA and first booster in IFA. The highest titer was found at eleventh bleeding after the third booster with prolactin isolate in IFA.
The Effect of Folic Acid as Supportive Therapy of Spiramycine on Weight of Foetus to Toxoplasma gondii – Infected Pregnant Mice (Mus musculus) Alfina Azkiana; Boedi Setiawan; Erma Safitri; Lucia Tri Suwanti; Mufasirin Mufasirin; Djoko Legowo
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.511 KB) | DOI: 10.20473/jops.v3i1.16432

Abstract

This research aimed to investigate the effect of folic acid as supportive therapy of spiramycine on weight of foetus to Toxoplasma gondii-infected pregnant mice (Mus musculus). Twenty pregnant female mice were divided into four groups as C -, C +, T1 and T2. C +, T1 and T2 were infected by Toxoplasma gondii. C – and C + administered orally 0.5 ml aquadest, T1 administered orally 130 mg/kg BW spiramycine and 0.052 µg/g BW folic acid and T2 administered orally 0.052 µg/g BW folic acid. Experimental groups received the treatments for 5 days, then animals of each groups were sacrified. Foetuses were dissected out for observation. The weight of fetuses were measured using an analytical balance. The data weight of foetuses was presented descriptively and analyzed by ANOVA test and continued by Tukey HSD. From this study, the weight of foetuses from the pregnant mice of T1 and T2 have difference compared with the controls. The result of this research is folic acid affects the weight of foetuses to Toxoplasma gondii-infected pregnant mice.
Identification of Gastrointestinal Endoparasite in Bawean Deer (Axis kuhlii) and Spotted Deer (Axis axis) at Bratang Flora Park – Surabaya Hartono Hartono; Endang Suprihati; Erma Safitri; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.868 KB) | DOI: 10.20473/jops.v3i2.16518

Abstract

The purpose of this study is to identify the endoparasites of Spotted Bawean Deer (Axis kuhlii) and Deer (Axis axis ) at Surabaya Flora Park. The research was conducted in December 2018 - January 2019 used total 60 specimen of sample faeces, consist of 30 sample of bawean deers faeces and 30 sample of spoted deers faeces, the samples analyzed at the Department of Parasitology Veterinary, Faculty of Veterinary Medicine, Universitas Airlangga. The results of the study found positive endoparasites on examination, samples of Bawean deer faeces (Axis kuhlii) and Spotted deer faeces(Axis axis) in Baratang Flora Park Surabaya found that 26 positive samples from 60 samples were obtained. Positive endoparasitic samples are protozoa at the stage of oocyst, namely Eimeria sp. 6 (10%) samples, worm eggs from the Nematoda class, Haemonchus sp. 20 (33.33%) and whereas endoparasites from other stages and classes were not found. The results of the analysis used Chi-Square test and showed no significantly differences degree of gastrointestinal endoparasite infection of Bawean deer (Axis kuhlii) and Spotted deer (Axis axis) (p>0.05).
Motilitas dan Viabilitas Spermatozoa Sapi Rambon di Desa Kemiren Banyuwangi Prastika, Zeni; Susilowati, Suherni; Agustono, Bodhi; Safitri, Erma; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.103 KB) | DOI: 10.20473/jmv.vol1.iss2.2018.38-42

Abstract

Sapi Rambon merupakan salah satu sapi lokal Indonesia. Tahun 2017 populasi sapi Rambon mengalami penurunan. Salah satu upaya pelestarian sapi lokal adalah melalui peningkatan populasi sapi dan inseminasi buatan. Faktor penentu keberhasilan perkawinan salah satunya adalah libido tinggi dan kualitas semen. Motilitas spermatozoa dan viabilitas spermatozoa adalah salah satu indikator fertilitas spermatozoa dan kualitas semen. Penelitain ini adalah penelitian deskriptif. Penelitian ini bertujuan untuk mengetahui motilitas dan viabilitas semen segar sapi Rambon di Desa Kemiren Kecamatan Glagah Kabupaten Banyuwangi. Semen pada penelitian ini di tampung menggunakan vagina buatan dari 6 ekor sapi Rambon jantan. Pemeriksaan motilitas spermatozoa dan viabilitas spermatozoa dilakukan di klinik Linawati Banyuwangi. Hasil pada penelitian ini disajikan dalam bentuk tabel. Parameter penelitian ini adalah motilitas dan viabilitas spermatozoa sapi Rambon. Hasil pemeriksaan motilitas spermatozoa sapi Rambon 83,3±4,1% dan viabilitas spermatozoa sapi Rambon 86,7±2,3%. Kesimpulan dari penelitian menunjukan bahwa motilitas dan viabilitas spermatozoa sapi Rambon baik dan memenuhi syarat semen beku untuk inseminasi buatan.
Pengaruh Ekstrak Kedelai (Glycine max) Terhadap Proliferasi Lapisan Endometrium Mencit (Mus musculus) Salahuddin, Muhammad Soleh; Safitri, Erma; Yunita, Maya Nurwartanti; Susilowati, Suherni; Hamid, Iwan Sahrial; Yudhana, Aditya
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.497 KB) | DOI: 10.20473/jmv.vol2.iss1.2019.49-54

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh ekstrak kedelai untuk meningkatan proliferasi lapisan endometrium dalam proses perkembangan organ uterus pada mencit (Mus musculus) betina. Kelompok kontrol (K) tidak diberi perlukaan. Kelompok P1 diberi ekstrak kedelai dengan dosis 12,5mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 25mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 37,5mg/kgBB. Kelompok P4 diberi ekstrak kedelai dengan dosis 50mg/kgBB. Perlakuan dilakukan setiap hari sekali selama empat belas hari. Hasil uji One Way Anova menunjukkan perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Ketebalan endometrium kelompok P2, P3, dan P4 berbeda secara nyata (p<0,05), dengan kelompok kontrol (K), dan kelompok kontrol (K) tidak berbeda nyata dengan kelompok P1 (p>0,05), serta kelompok P1 tidak berbeda nyata dengan kelompok P2 (p>0,05). Kandungan senyawa fitoestrogen yaitu isoflavon berupa daidzein dan genistein bekerjasama dalam mengoptimalkan proliferasi lapisan endometrium pada uterus mencit (Mus musculus) betina. Daidzein bekerja sebagai senyawa estrogenik untuk berikatan dengan reseptor estrogen pada uterus dan genistein bekerja sebagai penghambat pertumbuhan lapisan endometrium yang berlebih pada jumlah tertentu. Kesimpulan dari penelitian ini adalah ekstrak kedelai (Glycine max) efektif untuk peningkatan proliferasi lapisan endometrium pada uterus mencit betina.
Tingkat Keberhasilan Penetasan Telur Penyu Lekang (Lepidochelys olivacea) pada Sarang Semi Alami di Pantai Boom Banyuwangi Periode Tahun 2018 Umama, Ayu Riza; Restiadi, Tjuk Imam; Prastiya, Ragil Angga; Safitri, Erma; Saputro, Amung Logam; Yudhana, Aditya; Haditanojo, Wiyanto
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.255 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.17-24

Abstract

Penelitian ini bertujuan mengetahui persentase keberhasilan penetasan telur penyu lekang (Lepidoehelys olivacea) pada sarang semi alami Banyuwangi sea Turtle Foundation (BSTF) di Pantai Boom Banyuwangi. Data diperoleh dari observasi dan rekap data milik BSTF. Persentase keberhasilan penetasan diperoleh dari total telur penyu lekang selama periode bertelur tahun 2018. Persentase penetasan telur penyu lekang pada sarang semi alami BSTF periode tahun 2018 adalah 75%. Total telur penyu yang diinkubasikan adalah 4495 butir, total telur yang menetas adalah 3392 butir, dan jumlah telur yang gagal menetas adalah 1103 butir.
Difference Time Effect of Equilibration Before Freezing on The Quality of Spermatozoa Sapera Goats using Egg Yellow Tris Prayogo, Danu; Susilowati, Suherni; Prastiya, Ragil Angga; Safitri, Erma; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.188-195

Abstract

This study aimed to examine the effect of time differences on motility, viability, abnormality, and intact plasma membrane of spermatozoa of Sapera goats before freezing. This study used a Completely Randomized Design (CRD) with three settings, namely the equilibration time P1 (1 h), P2 (3 h), and P3 (4 h), each of six replications. The results of motility using One Way Anova statistical analysis through the Duncan Test were (P1) 71.50 ± 3.94, (P2) 66.00 ± 2.60, and (P3) 60.33 ± 2.42. The results of viability was (P1) 83.00 ± 6.16, (P2) 75.17 ± 4.70, and (P3) 69.00 ± 3.40. Abnormal results was (P1) 2.50 ± 0.55, (P2) 3.33 ± 1.18, and (P3) 6.50 ± 2.88. While the results of intact plasma membranes were (P1) 79.50 ± 8.50, (P2) 72.67 ± 5.28, and (P3) 65.67 ± 3.78. The conclusion of the study showed significant significant results (p<0,05) between the equilibration time and spermatozoa quality of Sapera goats before freezing. The highest presentation of motility, viability and overall plasma membrane was at an equilibration time of 1 h, while the lowest abnormality presentation was at equilibration time of 1 h.
Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test Kusuma, Agung Jati; Safitri, Erma; Praja, Ratih Novita; Tyasningsih, Wiwiek; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.199-206

Abstract

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.
Co-Authors Agustono, Bodhi Al arif, Mohammad Anam Alfanindya, Evita Aisyah Alfina Azkiana Amrullah, Muhammad Fajar Annise Proboningrat Boedi Setiawan Budi Utomo Chandramohan, Koshini Darsono, Roesno Dikman, Dicky Mohammad Djoko Legowo Eha Renwi Astuti Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Ercha Aulia Putri, Cindy Erlangga, Made Bagus Ernie Maduratna Setiawati Firdaus, Muhammad Aviv Haditanojo, Wiyanto Hani Plumeriastuti Hardaningtyas, Enggar Hartono Hartono Hasib, Abdullah Heni Puspitasari Herry Agoes Hermadi Hidanah, Sri Husni Anwar Iis Aprinawati, Iis Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Inggriani, Fauzia Iwan Sahrial Hamid Khairullah, Aswin Rafif Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Lisnanti, Ertika Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mafruchati, Maslichah Mufasirin Nunuk Dyah Retno Lastuti Nusdianto Triakoso Pasang, Gabriel Sampe Praja, Ratih Novita Prastika, Zeni Prastiya, Ragil Angga Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Putri, Natalie Lovena Pramono Rachmani, Sartika Dewi Raharjo, Dadik Rao, Sherbrina Bai Seenivasa Ratna Damayanti Restiadi, Tjuk Imam Rimayanti Rimayanti Rimayanti Salahuddin, Muhammad Soleh Santoso, Kuncoro Puguh Saputro, Amung Logam Seagames Waluyo, Seagames Shabira, Zahra Silfia, Himatul Ilma Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati, Sri Pantja Sri Wigati Mardi Mulyani Suherni Susilowati Supriyadi Supriyadi Suzanita Utama Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tita Damayanti Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Umama, Ayu Riza Viora, Dwi Viski Fitri Hendrawan Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Winda Syafitri, Winda Wurlina, W Yasmidas, Yasmidas Yudhana, Aditya Yunita, Maya Nurwartanti Yunus, Muchammad Zulfarniansyah, Akhmad Baihaqi