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Selection of early maturing and high yielding mutants of Toraja Local Red Rice Grown from M2-M3 Population after Ion Beam Irradiation Sjahril, Rinaldi; Trisnawaty, A. R.; Riadi, Muhammad; Rafiuddin, Rafiuddin; Sato, Tadashi; Toriyama, Kinya; Hayashi, Yoriko; Abe, Tomoko
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

This study aims to obtain the genotype of local Toraja red rice M2 mutants that have the potential to be developed into early maturing varieties and have high yields. Research carried out with a mass selection method consisting of two irradiation treatments, namely: (1) irradiation with a Carbon ion dose of 150 Gy (PL-C); (2) irradiation with Argon ion dose of 10 Gy (PL-A) and control as a comparison. Selected strains of M2 seed population were planted in experimental strains plus 2 strains as control, each row had 50 plants. Selection is done in two stages. The first stage, the selection is based on the criteria of plant growth components, to select rice plants that have early-middle age characters, dwarf-semi-dwarf, and many tillers. The second stage, selected strains from the scoring method were then grouped based on the degree of similarity with the cluster analysis method. Irradiation with ion beams produces mutants with shorter harvest times than their parents, with the harvest time 16-17 days or 10% shorter compared to their parents. This study produced 10 selected strains, 5 strains of carbon ion and 5 strains of argon ion irradiation treatment, and formed 5 groups with a 90% similarity coefficient.

Selection of early maturing and high yielding mutants of Toraja Local Red Rice Grown from M2-M3 Population after Ion Beam Irradiation Rinaldi Sjahril; A. R. Trisnawaty; Muhammad Riadi; Rafiuddin Rafiuddin; Tadashi Sato; Kinya Toriyama; Yoriko Hayashi; Tomoko Abe
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

This study aims to obtain the genotype of local Toraja red rice M2 mutants that have the potential to be developed into early maturing varieties and have high yields. Research carried out with a mass selection method consisting of two irradiation treatments, namely: (1) irradiation with a Carbon ion dose of 150 Gy (PL-C); (2) irradiation with Argon ion dose of 10 Gy (PL-A) and control as a comparison. Selected strains of M2 seed population were planted in experimental strains plus 2 strains as control, each row had 50 plants. Selection is done in two stages. The first stage, the selection is based on the criteria of plant growth components, to select rice plants that have early-middle age characters, dwarf-semi-dwarf, and many tillers. The second stage, selected strains from the scoring method were then grouped based on the degree of similarity with the cluster analysis method. Irradiation with ion beams produces mutants with shorter harvest times than their parents, with the harvest time 16-17 days or 10% shorter compared to their parents. This study produced 10 selected strains, 5 strains of carbon ion and 5 strains of argon ion irradiation treatment, and formed 5 groups with a 90% similarity coefficient.

Identifikasi Senyawa Kimia pada Tanaman Pulai (Alstonia scholaris) Sebagai Pestisida Nabati untuk Pengendali Hama Rifni Nikmat Syarifuddin; Trisnawaty AR; Andi Nurwidah
JURNAL GALUNG TROPIKA Vol 10 No 1 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i1.701

Efektivitas Pemanfaatan Lahan Pekarangan dalam Mendukung Ketersediaan Pangan pada Era Pandemi Covid-19 di Pedesaan Reza Asra; Trisnawaty AR
JURNAL GALUNG TROPIKA Vol 10 No 3 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i3.856

National food security is a problem that must be tackled together, especially in the new normal era caused by the covid 19 pandemic. The yard of the house to produce food is one of the concepts of using yard land both in rural and urban areas to support national food security by empowering potential local food. The yard is not only to create beauty and coolness, but also to improve the economy of each family. The purpose of this study was to determine the characteristics of the community in the use of yard land during the Covid-19 pandemic, to determine the pattern of yard use, and to determine the effectiveness of using yard land in rural areas, especially residents of Carawali Village, Sidrap Regency, in supporting food availability. The data analysis used in this study was an experiment with a one group pre-test post-test design model. In this design, an initial measurement (pre-test) was carried out, after which certain treatment was given, then a re-measurement was carried out (post-test). Sampling in this study using snowball sampling. The results showed that the use of yard land in supporting the food availability of residents in Carawali Village, Watang Pulu District showed that the percentage of the answer scores for the program effectiveness variable described from several questionnaire questions to respondents was in the range of values of 50 - 100%, which means that it is included in the effective criteria.

Pelatihan Budidaya Rumput Unggul di Kelurahan Massepe Kabupaten Sidrap Muh. Irwan; Reza Asra; Aksal Mursalat; Trisnawaty Trisnawaty
Abdimas Singkerru Vol. 2 No. 1 (2022)
Publisher : Akademi Teknologi Industri Dewantara Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar

Tujuan pengabdian ini adalah untuk meningkatkan kompetensi peternak dalam hal budidaya rumput unggul secara menyeluruh. Kegiatan ini dilaksanakan pada Kelompok Ternak Jaya Bersama yang berkedudukan di Kelurahan Massepe Kecamatan Tellu Limpoe Kabupaten Sidenreng Rappang Provinsi Sulawesi selatan. Metode pelaksanaan kegiatan yang digunakan adalah Participatory Rural Appraisal (PRA). Metode ini adalah serangkaian atau sekelompok kegiatan dengan metode yang memungkinkan mitra dapat mengamati, mengkaji, saling berbagi, meningkatkan dan menganalisis pengetahuan tentang kondisi dan aspek-aspek yang berkaitan untuk menunjang kreativitas dan kemandirian peternak dalam menyediakan pakan berbahan baku lokal yang berkualitas. Tahapan kegiatan ada 3 yakni sosialisasi, pelatihan budidaya, dan evaluasi kegiatan. Kesimpulan dari kegiatan ini adalah Kegiatan dinilai efektif berdasarkan hasil evaluasi yang mendapatkan bobot penilaian sebesar 87,71. Nilai tersebut menunjukkan kegiatan pengabdian yang dilaksanakan dikategorikan berhasil pada aspek peningkatan pengetahuan dan keterampilan yang berorientasi pada aspek kompetensi teknis peserta. Kegiatan ini layak dilanjutkan untuk implementasi lapangan dengan skala yang lebih besar.

Performance of NAA, 2iP, BAP and TDZ on Callus Multiplication, Shoots Initiation and Growth for Efficient Plant Regeneration System in Chrysanthemum (Chrysanthemum morifolium Ramat.) Rinaldi Sjahril; Feranita Haring; Muhammad Riadi; Muhammad Danial Rahim; Raham Sher Khan; Arjunayanti Amir; Trisnawaty A. R.
International Journal of Agriculture System VOLUME 4 ISSUE 1, JUNE 2016
Publisher : Hasanuddin University

Three series of plant growth regulators experiments in Chrysanthemum tissue culture, each arranged in completely randomized design (CRD), were studied to formulate efficient plant regeneration system for developing Chrysanthemum plant cell engineering. In the first experiment, 100 mg callus were cultured in liquid Murashige and Skoog (MS) medium supplemented with different combinations of NAA and BAP concentrations treatments. After 30 days of culture, heaviest callus (1.55 g) was obtained in NAA (1.0 mg L-1) + BAP (0.5 mg L-1). Consequently, shoot regeneration experiment with different kinds and concentrations of auxin, i.e. NAA and 2iP were each combined with different concentrations of cytokine BAP. Callus grown on solid MS medium supplemented with both NAA and BAP at 0.5 mg L-1 showed fastest shoot initiation (30 days), largest number of shoots (5 shoots), longest shoot length (2.88 cm). While combination of 2iP (0.5 mg L-1) and BAP (2.0 mg L-1) also produced same response on shoot initiation but shorter (1.88 cm) and only one shoot. Largest number of shoots (3 shoots) was obtained in treatment BAP (0.5 mg L-1) without 2iP, although shoot initiation was slower (39 days) with shorter length (1 cm). In a separate experiment, effect of single treatment of potential synthetic cytokine for shoot regeneration, Thidiazuron (TDZ), at different concentrations was examined. However, callus grown on TDZ incorporated medium did not produce any shoots and changed from green to brown at end of study (90 days). It was assumed that TDZ inhibited formation of shoots in Chrysanthemum callus culture.

Efektivitas Pemanfaatan Lahan Pekarangan dalam Mendukung Ketersediaan Pangan pada Era Pandemi Covid-19 di Pedesaan Reza Asra; Trisnawaty AR
JURNAL GALUNG TROPIKA Vol 10 No 3 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i3.856

National food security is a problem that must be tackled together, especially in the new normal era caused by the covid 19 pandemic. The yard of the house to produce food is one of the concepts of using yard land both in rural and urban areas to support national food security by empowering potential local food. The yard is not only to create beauty and coolness, but also to improve the economy of each family. The purpose of this study was to determine the characteristics of the community in the use of yard land during the Covid-19 pandemic, to determine the pattern of yard use, and to determine the effectiveness of using yard land in rural areas, especially residents of Carawali Village, Sidrap Regency, in supporting food availability. The data analysis used in this study was an experiment with a one group pre-test post-test design model. In this design, an initial measurement (pre-test) was carried out, after which certain treatment was given, then a re-measurement was carried out (post-test). Sampling in this study using snowball sampling. The results showed that the use of yard land in supporting the food availability of residents in Carawali Village, Watang Pulu District showed that the percentage of the answer scores for the program effectiveness variable described from several questionnaire questions to respondents was in the range of values of 50 - 100%, which means that it is included in the effective criteria.

Effect of Osmo-Priming with Polyethylene Glycol 6000 (PEG-6000) on Rice Seed (Oryza sativa L.) Germination and Seedling Growth Under Drought Stress Trisnawaty AR
International Journal of Agriculture System VOLUME 9 ISSUE 1, JUNE 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v9i1.4258

One of the efforts to increase rice production is by extensive farming through the utilization of marginal lands affected by drought. The present study aims to examine the influence of seed priming with PEG 6000 on rice seed germination and growth of rice seedlings in drought conditions and to group the rice varieties tested based on their tolerance to drought, and determine priming formulations for early adaptation of rice seedsthat can be used by farmers and industry. The research was organized in the form of a Split Plot Design with Randomized Group Design. TheMain Plot is a rice variety, consisting of 10 levels, i.e., Inpari 31, Inpari 33, Inpari 36, Inpari 39, Inpari 40, Inpago 8, Inpago 10, Mekongga,Rindang 1 and Rindang 2. While the Split Plot is a concentration of PEG, which consists of 5 levels, i.e., without soaking as negative controlconcentration 0 g L-1 PEG as positive control, and 50, 100, and 150 g L-1 PEG-6000. Each of these treatments is repeated three times, so thereare 150 observation units. Results showed that among the 10 varieties tested, there were 2 less tolerant varieties with scores of 5-10 (Inpari 31and Inpari 39), 5 varieties are quite tolerant with a score of 11-16 (Inpari 33, Inpari 36, Inpari 40, Inpagi 10 and Mekongga) and 3 varieties are very tolerant with a score of 17-22 (Rindang 1, Rindang 2 and Inpago 8) with priming concentrations of 100 g L-1 PEG-6000 for 36 hours.

Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis Rinaldi Sjahril; Muh. Riadi; Ifayanti Ridwan Saleh; Novitasari Novitasari; Ernitha A. Galla; Kasmiati Kasmiati; A. R. Trisnawaty; Nurhaya J. Panga
AGRIVITA, Journal of Agricultural Science Vol 45, No 2 (2023)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v45i2.3633

Katokkon pepper plant is originated from Toraja, South Sulawesi, Indonesia. This endemic pepper variety has unique hot and spicy characters with a distinctive bell pepper aroma, and aesthetic shape similar to paprika, but with smaller size. This research was conducted to identify ploidy level and plant morphology of colchicine induced Katokkon pepper at Laboratory of Plant Reproduction Bioscience and Biotechnology, Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin and experimental field of Agricultural Extension System Vocational High School Santo Paulus Tana Toraja (S 03Â°04â€™177â€ E 119Â°51â€™526â€). Two weeks old seedlings were immersed for 4.5 hours in colchicine concentration (0.00%, 0.0125%, 0.025%, 0.05% and 0.10%). Flow cytometry analysis was carried out using Partec Cy-Flow SpaceTM. Result showed that colchicine concentration (0.025%, 0.05% and 0.1%) produced mixoploid plants with two set of chromosomes (2n=24, 4n=48). This study also found morphological differences between mixoploids plants induced by 0.025%, 0.05% and 0.1% colchicine and diploid plants (0% and 0.0125% colchicine) during first two juvenile leaves phase. However, this difference did not occur further, and eventually morphology of adult mixoploid plant was not significantly different from control (diploid), which concurred to grading mixoploid grade 2. The mixoploid plants analyzed consist of higher diploids cells than tetraploid.

PELATIHAN BELAJAR BAHASA INGGRIS UNTUK ANAK SDN 124 JALIKKO, TALLU BAMBA, KEC. ENREKANG, KABUPATEN. ENREKANG PROV. SULAWESI SELATAN Sam Hermansyah; Jamaluddin Ahmad; Muhammad Rais Rasak; Ahmad Mustanir; Muhammad Hanafi; Trisnawaty Trisnawaty; Suleha Suleha
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 4 (2023): Volume 4 Nomor 4 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i4.18631

Tujuan dari program bimbingan belajar bahasa Inggris adalah untuk membantu anak-anak membiasakan diri dengan bahasa Inggris dasar dan mampu mengucapkan kata-kata dan frasa dasar bahasa Inggris yang relevan dengan kehidupan sehari-hari. Lokasi pengabdian masyarakat terkait bimbingan belajar bahasa Inggris siswa SD adalah RT 17 dan 20 RW VI, SD Negeri 124 Jalikko, Tallu Bamba, Kec. Enrekang, Kab. Enrekang Prov. Sulawesi Selatan. Layanan ini mencakup 20 siswa SD kelas 4-6 yang tinggal di RT 17 dan RW 20 VI, SD Negeri 124, Jalikco, Tallus Bamba. distrik Waktu yang dibutuhkan untuk kursus bahasa Inggris ini adalah tiga bulan. Metode penerapan partisipasi masyarakat dalam pembelajaran bahasa Inggris bagi siswa sekolah dasar adalah metode sosialisasi, metode pelatihan, dan metode pengajaran. Hasil dari pengabdian menunjukkan bahwa anak-anak sangat senang dan tertarik dengan program pengajaran bahasa Inggris. Pada awal sesi les bahasa Inggris, anak-anak mengalami kesulitan dalam mengucapkan kata-kata bahasa Inggris. Setelah pertemuan ketiga, mereka mulai mengucapkan kata-kata dengan benar, meskipun masih terdapat beberapa kesalahan dalam pengucapan. Metode pembelajaran yang digunakan untuk membimbing dan melatih anak antara lain bermain, bernyanyi, menggaung, bersuara, dan bermain peran. Hasil penelitian yang dilakukan terhadap siswa SD sebelum diinstruksikan menunjukkan bahwa dari 20 siswa SD yang mengikuti ujian, hanya satu yang lulus dan 19 gagal. IPK keseluruhannya adalah 57. Hal ini menunjukkan bahwa mereka harus mengikuti instruksi dalam bahasa Inggris. Hasil tes setelah belajar bahasa Inggris menunjukkan, dari 20 siswa SD yang mengikuti ujian, semuanya lulus. Hal ini terlihat dari nilai tesnya. Data menunjukkan bahwa 8 anak mempunyai nilai antara 60 sampai 69 dan 12 anak mempunyai nilai antara 70 sampai 79. Rata-rata nilai 20 anak adalah 70. Dengan demikian, les bahasa Inggris untuk anak SD dapat meningkatkan hasil belajar anak.

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