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Pengaruh Intervensi Terhadap Pengetahuan Farmasis Remaja Tentang Swamedikasi Yunita, Erma; Qonitah, Fadilah; Khasanah, Kharismatul; Zulbayu, L.M. Andi; Mistriyani, Mistriyani; Pratama, Nofran Putra; Jannah, Nurul; Sayakti, Putri Indah; Wulandari, Septi; Nur, Syamsu; Hertiani, Triana
Journal of Community Engagement and Empowerment Vol 1, No 1 (2019)
Publisher : Institut Ilmu Kesehatah Bhakti Wiyata Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar Belakang: Farmasis remaja memiliki peran dalam pembangunan kesehatan. Salah satu kegiatan yang dilakukan adalah penerapan swamedikasi. Tujuan: Pentingnya peran farmasis remaja dalam swamedikasi mendorong dilakukannya kegiatan pengabdian dengan tema pengenalan peran farmasis remaja dalam swamedikasi. Metode: Peserta diberikan pretest dan selanjutnya diberi intervensi berupa materi umum terkait swamedikasi, diskusi dan praktek swamedikasi bersama tutor. Media yang digunakan berupa materi dalam bentuk power point, modul dan alat peraga. Kegiatan diakhiri dengan melakukan posttest pada peserta yang sama. Hasil: Hasil pretest menunjukan bahwa hanya 14 peserta (20,29%) yang memiliki pengetahuan baik, sedangkan 50 peserta (72,46%) berpengetahuan cukup dan 5 peserta (7,25%) memiliki pengetahuan kurang. Nilai rata-rata hasil pretest adalah 57,10. Setelah diberi intervensi, hasil posttest menunjukan bahwa 66 peserta (95,65%) memiliki pengetahuan baik, sedangkan hanya sejumlah 3 peserta (4,35%) yang masih memiliki pengetahuan cukup. Nilai rata-rata posttes adalah 85,60. Kesimpulan: Adanya peningkatan nilai ini menunjukkan bahwa pemberian intervensi dalam program pengenalan farmasis remaja dalam swamedikasi yang diberikan dapat meningkatkan pengetahuan siswa SMK Pelita Bangsa Yogyakarta tentang swamedikasi. Perlu dilakukan penelitian sejenis dengan jangka waktu antara pretest dengan posttest lebih dari 1 bulan.
Total phenolic and flavonoid contents of n-hexane fraction in binjai leaves (Mangifera caesia Jack. ex. Wall) Cahaya Rahayu Yuliani; Hafiz Ramadhan; Putri Indah Sayakti; Cast Torizellia
Jurnal Ilmiah Farmasi 2022: Special Issue
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.specialissue2022.art2

Abstract

AbstractBackground: Binjai leaves (Mangifera caesia Jack. ex. Wall) or M. caesia contain phenolic and flavonoid compounds important in medicinal plants development. The solvent difference and fractionation will give various active substance concentrations.Objective: To evaluate the phenolic and flavonoid content qualitatively and quantitatively from the n-hexane fraction of binjai leaves methanol extract.Methods: The soxhletation method with methanol was used to extract binjai leaves followed by fractionation. Thin Layer Chromatography (TLC) followed by spraying reagents were used to test phenols and flavonoids compounds. Folin-Ciocalteu reagents were used to analyzed total phenolics, while AlCl3 reagents were used to quantify the total flavonoid content.Result: This study gave positive results on the qualitative test of phenolics and flavonoids. Meanwhile, the fraction assay results showed total phenolic levels of 45.19 µgGAE/mg and total flavonoid levels of 165.06 µgQE/mg.Conclusion: The n-hexane fraction of M. caesia leaves methanol extracts contains phenols and flavonoid content so that it has the potential as a medicinal plant.Keywords: Binjai leaves, methanol extracts, n-hexane fraction, phenol, flavonoid. IntisariLatar belakang: Daun binjai (Mangifera caesia Jack. ex. Wall) memiliki senyawa fenolik dan flavonoid yang penting dalam pengembangan tanaman obat. Perbedaan pelarut dan fraksinasi akan memberikan kadar senyawa aktif yang berbeda.Tujuan: Penelitian ini bertujuan untuk mengetahui secara kualitatif dan kuantitatif senyawa fenolik dan flavonoid dari fraksi n-heksan ekstrak metanol daun binjai.Metode: Metode soxhletasi dengan pelarut metanol digunakan untuk mengekstraksi daun binjai yang dilanjutikan dengan fraksinasi. Kromatografi Lapis Tipis (KLT) yang dilanjutkan dengan reagen semprot digunakan untuk menguji senyawa fenolik dan flavonoid. Reagen Folin-Ciocalteu digunakan untuk menetapkan kadar total fenolik, sedangkan pereaksi AlCl3untuk menetapkan kadar total flavonoid.Hasil: Penelitian ini memberikan hasil positif pada uji kualitatif fenolik dan flavonoid, sedangkan hasil penetapan kadar fraksi memberikan hasil total fenolik sebesar 45,19 µgGAE/mg dan kadar total flavonoid 165,06 µgQE/mg.Kesimpulan: Fraksi n-heksana ekstrak metanol daun M. caesia memiliki kandungan fenolik dan flavonoid sehingga berpotensi untuk dikembangkan sebagai tanaman obat,Kata Kunci: Daun binjai, ekstrak metanol, fraksi n-heksan, fenolik, flavonoid.
Antioxidant activity of methanol extract of cassava leaves (Manihot esculenta Crantz) using CUPRAC method Putri Indah Sayakti; Norma Anisa; Hafiz Ramadhan
Jurnal Ilmiah Farmasi 2022: Special Issue
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.specialissue2022.art12

Abstract

Background: Antioxidants are a group of organic molecules and enzymes that work synergistically to enhance cellular defenses and combat oxidative stress. Antioxidants can come from plants because they contain many groups of phytochemical compounds and vitamins. One of the plants that has compounds with antioxidant activity is cassava.Objective: To measure the antioxidant activity of methanol extract of cassava leaves using the CUPRAC method.Methods: Cassava leaves were extracted by maceration using methanol as a solvent with the addition of 5% H2SO4. Phytochemical screening of flavonoids with Mg and HCl, while phenol with the addition of FeCl3. The antioxidant activity based on the reaction of the test solution with CUPRAC reagent. Antioxidant activity can be determined by IC50 value by using x value as concentration and y value as absorbance.Results: The results of phytochemical screening showed that the methanol extract of cassava leaves contained flavonoids and phenolic groups. The antioxidant capacity of the methanol extract of cassava leaves obtained an IC50 value of 156.03 ppm, while quercetin has a very strong capacity as an antioxidant with an IC50 of 9.83 ppm.Conclusion: The methanol extract of cassava leaves has a weak antioxidant capacity in reducing metal ions. Keywords: Cassava leaves, Manihot esculenta Crantz, methanol extract, antioxidants, CUPRAC.IntisariPendahuluan: Antioksidan adalah kelompok molekul organik dan enzim yang bekerja secara sinergis untuk meningkatkan pertahanan seluler dan memerangi stres oksidatif. Antioksidan dapat berasal dari tanaman karena banyak mengandung golongan senyawa fitokimia dan vitamin. Salah satu tanaman yang memiliki kandungan metabolit sekunder berupa flavonoid dan fenol yang berfungsi sebagai antioksidan adalah Singkong (Manihot esculenta Crantz).Tujuan: Penelitian ini bertujuan untuk mengukur kapasitas antioksidan dari ekstrak metanol daun Singkong menggunakan metode CUPRAC (Cupric Ion Reducing Antioxidant Capacity).Metode: Daun Singkong diekstraksi secara maserasi menggunakan pelarut metanol dengan penambahan H2SO4 5%. Skrining fitokimia flavonoid dengan Mg dan HCl, sedangkan fenol dengan penambahan FeCl3. Kapasitas antioksidan berdasarkan reaksi larutan uji dengan reagen CUPRAC yang diukur menggunakan spektrofotometer UV-Vis.Hasil: Hasil skrining fitokimia menunjukkan ekstrak metanol daun Singkong mengandung golongan flavonoid dan fenolik. Kapasitas antioksidan dari ekstrak metanol daun Singkong didapatkan nilai IC50 sebesar 156,03 ppm, sedangkan quersetin memiliki kapasitas yang sangat kuat sebagai antioksidan dengan IC50 sebesar 9,83 ppm.Kesimpulan: Berdasarkan hasil tersebut, dapat disimpulkan bahwa ekstrak metanol daun Singkong memiliki kapasitas antioksidan yang lemah dalam mereduksi ion logam.Kata kunci: Daun Singkong (Manihot esculenta Crantz), Ekstrak Metanol, Antioksidan, CUPRAC.
Pemberian Edukasi Bahaya Radikal Bebas melalui Pengolahan Minuman Kesehatan Lidah Buaya pada Penghuni Rumah Yatim Ar-Rohmah Banjarbaru Kalimantan Selatan Rahmi Hidayati; Ratna Restapaty; Putri Indah Sayakti
Mitra Mahajana: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2021): Volume 2 Nomor 2 Tahun 2021
Publisher : LPPM Universitas Flores

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/mahajana.v2i2.870

Abstract

Excess free radicals in the body to exceed the body's ability to manage it can cause a condition called oxidative stress. The impact of free radicals can attack and cause damage to various cells of the body. Antioxidants play a very important role for the health of the human body because their function can inhibit and neutralize the occurrence of oxidation reactions involving free radicals (Parwata, 2016). Antioxidants in food or beverages can contain natural antioxidants such as in vegetables, fruits and beverages as well as synthetic antioxidants that are deliberately added to the food and beverages consume. The problem in the orphanage environment becomes a challenge as well as an opportunity to provide education related to the dangers of free radicals and the processing of aloe vera drinks. Method of implementation of activities by providing education on the dangers of free radicals and demo processing aloe vera drink (Aloe Vera). The results of data analysis from questionnaires shared with orphaned residents showed that as many as 94.7% of people who had never followed counseling about the dangers of free radicals and aloe vera drink processing. Although many benefits are obtained from aloe vera, but the level of public consumption of aloe vera is very minimal, especially in the form of drinks. Processed products from aloe vera in the form of drinks containing antioxidants as a neutralizer of free radicals is very good, because beverage products are easier to digest by the body and the content in aloe vera (Aloe Vera) is still natural because it has not undergone many treatments. In addition, it is recommended to change high-risk habits and reduce the danger of free radicals by; 1) avoid being under UV light directly, 2) avoid excessive radiation from electronic devices, 3) avoid foods that are burned, smoked and fried excessively.
FENOL-FLAVONOID DAN AKTIVITAS ANTIOKSIDAN FRAKSI AIR DAN ETIL ASETAT DARI DAUN BINJAI (Mangifera caesia Jack. Ex. Wall) Hafiz Ramadhan; Putri Indah Sayakti; Rutbatul Ulya; Mahfuzah Hidayati; Zelita Perdani Putri; Abdul Rauf; Nafila Nafila
Jurnal Ilmiah Pharmacy Vol 9, No 1 (2022)
Publisher : Sekolah Tinggi Kesehatan Al-Fatah Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52161/jiphar.v9i1.378

Abstract

Binjai (Mangifera caesia Jack. Ex. Wall) adalah tanaman endemik Kalimantan yang berpotensi antioksidan. Penggunaan metanol sebagai pelarut dalam ekstraksi daun Binjai menghasilkan aktivitas antioksidan yang sangat kuat dibanding penggunaan etanol. Tujuan penelitian untuk mengetahui aktivitas antioksidan, kadar total fenol dan flavonoid pada fraksi etil asetat dan fraksi air dari ekstrak metanol daun Binjai. Daun binjai diekstraksi dengan metanol menggunakan soxhlet dan selanjutnya difraksinasi. Aktivitas antioksidan secara kualitatif dan kuantitatif diuji dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Kadar total fenol dan flavonoid ditentukan secara spektrofotometri menggunakan reagen Folin-Ciocalteu dan AlCl3. Hasil penelitian menunjukkan fraksi air (782 ± 0,983 mg GAE/g fraksi) memiliki kadar total fenol lebih tinggi dibandingkan fraksi etil asetat (430 ± 0,288 mg GAE/g fraksi). Sedangkan kadar total flavonoid fraksi etil asetat (274,32 ± 0,082 mg QE/g fraksi) lebih tinggi dari fraksi air (118,8 ± 0,013 mg QE/g fraksi). Aktivitas antioksidan dengan Kromatografi Lapis Tipis pada kedua fraksi menghasilkan noda kuning berlatarbelakang ungu setelah disemprot DPPH. Fraksi etil asetat menunjukkan aktivitas peredaman DPPH yang sangat kuat (IC50 = 5,356 ppm) dibandingkan fraksi air (IC50 = 23,175 ppm). Kesimpulannya yaitu fraksi etil asetat dan fraksi air dari ekstrak metanol daun Binjai menghasilkan aktivitas antioksidan yang sangat kuat dapat dikembangkan menjadi obat tradisional.
Uji Aktivitas Antioksidan Fraksi N-Heksan dari Ekstrak Metanol Daun Binjai Mangifera caesia Jack. Ex. Wall Menggunakan Metode DPPH Hafiz Ramadhan; Siti Purnama; Putri Indah Sayakti
JURNAL ILMU KEFARMASIAN INDONESIA Vol 20 No 1 (2022): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v20i1.1133

Abstract

Natural antioxidant compounds can be found in plants of the Mangifera genus, one of them is Mangifera caesia, Jack. ex. Wall or which was also known as binjai. This study aims to determine group compounds and the antioxidant activity of the n-hexane fraction from the binjai leaves methanol extract against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The binjai leaves powder extraction was carried out using the soxhlet apparatus with methanol and liquid-liquid fractionation using water and n-hexane. Phytochemical screening was done by adding specific reagents. The antioxidant activity assay qualitatively on TLC that being sprayed with DPPH and quantitative assay by measuring the absorbance of the fraction attenuation against DPPH radicals using a UV-Vis Spectrophotometer. The Phytochemicals screening results showed that the n-hexane fraction contained phenolic, flavonoid, and steroids. The qualitative test results showed some compounds had an antioxidant activity that appeared by changing the color of the spots to yellow on a purple background after spraying with DPPH solution. The n-hexane fraction resulted in an IC50 value of 34.0668 ppm with quercetin IC50 of 2.4469 ppm. Based on these results, it can be concluded that the n-hexane fraction of binjai leaves methanol extract contains phenolic and flavonoid compounds that played a role in producing very strong antioxidant activity.
SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70% BIJI KALANGKALA (Litsea angulata Bl.) TERHADAP BAKTERI PENYEBAB JERAWATPropionibacterium acnes Hafiz Ramadhan; Muhammad Arsyad; Putri Indah Sayakti
BORNEO JOURNAL OF PHARMASCIENTECH Vol 4 No 1 (2020): Borneo Journal of Pharmascientech
Publisher : Sekolah Tinggi Ilmu Kesehatan Borneo Lestari Banjarbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51817/bjp.v4i1.283

Abstract

Acne vulgaris or acne is a chronic inflammatory disease in the polysebaseus unit thatoften occurs especially in teenagers and adults, one of which is caused by thebacterium Propionibacterium acnes. Natural material that has the potential as anantibacterial is Kalangkala (Litsea angulata Bl.), where some people in SouthKalimantan use the seeds to treat boils. This research purposes to determine thecontent of secondary metabolites of 70% ethanol extract of Kalangkala seedsandtheir activities as antibacterial againts P.acnes. The phytochemical screening testincludes flavonoids, alkaloids, saponins, steroids-terpenoids and tannins. The methodof testing the antibacterial activity with the well diffusion method using extractconcentration 100%; 50%; 25%; 12.5%; 6.25%; and 3.125%. The results showed70% ethanol extract of Kalangkala seeds containing flavonoids, alkaloids, saponinsand tannins. The antibacterial activity of 70% ethanol extract of Kalangkala seedsagaints Propionibacterium acnes bacteria with well diffusion method was obtainedby MIC that is 25% with an average inhibition zone diameter of 8,667 mm whichincluded the moderate category as an antibacterial agent.
Identification of Essential Oils From The Bark of Balik Angin (Alphitonia Incana (Roxb.) Teijsm. & Binn. Ex Kurz) Tika Nurjanah; Hafiz Ramadhan; Nella Faradillah; Putri Indah Sayakti; Dyera Forestryana; Nafila Nafila
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 4, No. 1 (2022)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v1i1.42498

Abstract

One of the components that make up essential oils is phenolic compounds which are widely containedin the leaves and bark of the Balik Angin plant (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz).This study aimed to identify phenolic compounds and antioxidant activity of essential oils from thebark of the Balik Angin plant. The essential oils isolation using hydrodistillation and the compoundswere analyzed using gas chromatography-mass spectroscopy (GC-MS). Qualitatively antioxidantactivity test used thin layer chromatography (TLC) and quantitatively by measuring the absorbance of2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals residue that did not react against compounds reducer.The results showed the essential oils contain major compounds which are methyl salicylate followedby methyl palmitate, 2,2-Dimethyl-1,3-dioxane-4,6-dione, and methyl 12-acetoxyoleate. Qualitativetest results of antioxidant activity with TLC there were four yellow spots on a purple background. Thescavenging activity showed the essential oils had an IC50 of 229.056 ppm, while the gallic acid as acomparative standard obtained an IC50 of 0.618 ppm. The conclusion is the essential oils of BalikAngin bark that produces very weak antioxidant activity.
Pemberdayaan Masyarakat melalui Pemanfaatan Daun Insulin sebagai Produk Pangan Antidiabetes di PKK Cempaka Banjarbaru: Community Empowerment through on the Use of Insulin Leaves as Antidiabetic Food Products to PKK Group Cempaka Banjarbaru Putri Indah Sayakti; Ratna Restapaty; Revita Saputri; Lisa Setia; Reny Marliadi; Depy Oktapian Akbar
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 8 No. 3 (2023): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v8i3.4552

Abstract

The quality of information on health, including knowledge of plants that may be used as diabetes medicine, has a significant effect on public concern for health. This activity aims to introduce the community to the benefits of insulin leaves and Amazon beans, along with their benefits for diabetes, through community empowerment activities. Community empowerment activities include training in developing superior products with economic value using insulin leaves and Amazon beans. The problems identified include residents' need for more knowledge and skills in processing food based on insulin leaf plants, especially regarding food processing with antidiabetics. The method of activity used is education on the efficacy of insulin leaf plants and Amazon beans thru a Group Discussion Forum (FDG) with PKK RT 30 RW 20 women of Cempaka Banjarbaru Village. Moreover, the Community Empowerment Team provided training in the preparation using insulin leaves and Amazon beans into straightforward preparations such as pudding, cookies, tea, simplistic, simple steeping, and packaging manufacturing. Individuals could convert processed food products into Cempaka village home industry products as a business opportunity, thereby increasing people's living environment.
Kajian in silico aktivitas antioksidan-antikanker senyawa fenolik dan flavonoid spesies mangifera menggunakan molecular docking PLANTs: Bahasa Indonesia Hafiz Ramadhan; Dyera Forestryana; Putri Indah Sayakti; Mahmud Riyad; Ratna Restapaty
Jurnal Ilmiah Farmasi 2023: Special Issue
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.specialissue2023.art2

Abstract

Background: The development of science and technology has created a study of anticancer drug discovery through molecular docking. This method can be applied to screening natural compounds that have antioxidant properties as anticancer candidates.Objective: The aim of the in-silico study is to find out the potential antioxidant-anticancer activities using molecular docking of phenolic and flavonoid compounds contained in the Mangifera species.Methods: Mangiferin, homomangiferin, isomangiferin, quercitrin, kaempferol 3-O-glucoside, catechin, epicatechin, daidzein, genistein, α-tocopherol, gallic acid as the test compounds and Vitamin C, doxorubicin, and hydroxyurea as comparison were prepared with MarvinSketch. The targeted protein data bank (PDB) codes used are 1V4S, 1XAN, 2BEL, 4K7O, 5M2F, 6COX, and 2W3L which were prepared with YASARA. The prepared compounds and proteins docked with each other using PLANTs software.Result: The in-silico results showed that only vitamin C can exceed the native ligand docking against the 1V4S receptor. α-tocopherol has a better binding affinity compared to vitamin C on 1XAN, 2BEL, and 5M2F but could not reach the native ligand score. All of the test compounds have potential antioxidant activity against the 4K7O protein receptor, but α-tocopherol is the only one that has the ability to inhibit the 6COX protein receptor. α-tocopherol also has better anticancer activity against breast cancer initiator (2W3L) compared to other test compounds, doxorubicin, hydroxyurea, and native ligands. Conclusion: The conclusion is that α-tocopherol has the most potential as an antioxidant and anticancer candidate through in silico studies.