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All Journal HAYATI Journal of Biosciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Biodiversity and Biotechnology TREUBIA JURNAL BIOLOGI INDONESIA Tropical Genetics Berkala Ilmiah Biologi
Tuty Arisuryanti, Tuty
Laboratory of Genetics, Faculty of Biology, Gadjah Mada University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Analisis Kariotipe Ular Trawang (Coelognathus radiatus, (Boie 1827)) Qurniawan, Tony Febri; Arisuryanti, Tuty; Nur Handayani, Niken Satuti
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3047

Abstract

Analysis of Radiated Ratsnakes (Coelognathus radiatus, (Boie 1827) Karyotype. Radiatedratsnake is a reptile which has an economic potential an export comodyty. However, there arefew reports on genetic studies of radiated ratsnake. The aim of this research was to examinechromosome characters of the radiated ratsnake collected from Sewon, Bantul, Daerah IstimewaYogyakarta. Chromosome preparation method used was splash using blood culture. The resultsrevealed that the diploid chromosome number (2n) of radiated ratsnake was 30, classified asmetacentric (chromosome pairs number 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, dan 11), submetacentric(chromosome pairs number 12 and 13), and telocentric (chromosome pairs number 14 and 15).Therefore the karyotype formula of radiated ratsnake was 2n = 2x = 30 = 22 m + 4 sm + 4 t. Theresult showed that the shortest of short arm chromosomes was 0 ?m, the longest one was0.665 ± 0.0504 ?m, the shortest of long arm chromosomes was 0.268 ± 0.0011?m, the longestone was 0.746 ± 0.0059 ?m, the shortest of total length chromosomes was 0.27 ± 0.001 ?m, andthe longest of total length chromosomes was 1.41 ± 0.045 ?m. The R value was 5.267 ± 0.15408indicating that radiated ratsnake from Sewon, Bantul, Daerah Istimewa Yogyakarta haschromosome variation size.Keywords: Coelognathus radiatus, blood culture, chromosome character, karyotype
A new species of Microhyla (Anura: Microhylidae) from Sumatra, Indonesia Atmaja, Vestidhia Yunisya; Hamidy, Amir; Arisuryanti, Tuty; Matsui, Masafumi; Smith, Eric N.
TREUBIA Vol 45 (2018): Vol. 45, December 2018
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/treubia.v45i0.3625

Abstract

A new species of frog in the genus Microhyla is described from Sumatra, Indonesia based on molecular and morphological characters. This new species was previously confused with M. achatina, a Javan endemic. This new species is diagnosable from its congeners by possessing a medium size (SVL in adult males 18.20–21.32 mm, in adult females 20.37–25.51 mm), a stout body, a nostril–eyelid length being about half of the snout length, having a single outer palmar tubercle, a tibiotarsal articulation reaching the center of the eye (when the hindlimbs are stretched and adpressed to the body), having finger and toe tips dilated, having the dorsum with medial longitudinal grooves, and excibiting a very thin and short dark stripe on the temporal region above a wider cream stripe, extending from the postorbital area to insertion of forelimb. Additionally, the new species is characterized by possessing relatively little foot webbing. Uncorrected 16S rRNA sequence divergences between the new taxon and sequences for other congeneric species available ranged from 4.8 to 15.0%.
ANALISIS KARIOTIPE ULAR TRAWANG (COELOGNATHUS RADIATUS, (BOIE 1827)) Qurniawan, Tony Febri; Arisuryanti, Tuty; Nur Handayani, Niken Satuti
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3047

Abstract

Analysis of Radiated Ratsnakes (Coelognathus radiatus, (Boie 1827) Karyotype. Radiatedratsnake is a reptile which has an economic potential an export comodyty. However, there arefew reports on genetic studies of radiated ratsnake. The aim of this research was to examinechromosome characters of the radiated ratsnake collected from Sewon, Bantul, Daerah IstimewaYogyakarta. Chromosome preparation method used was splash using blood culture. The resultsrevealed that the diploid chromosome number (2n) of radiated ratsnake was 30, classified asmetacentric (chromosome pairs number 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, dan 11), submetacentric(chromosome pairs number 12 and 13), and telocentric (chromosome pairs number 14 and 15).Therefore the karyotype formula of radiated ratsnake was 2n = 2x = 30 = 22 m + 4 sm + 4 t. Theresult showed that the shortest of short arm chromosomes was 0 ?m, the longest one was0.665 ± 0.0504 ?m, the shortest of long arm chromosomes was 0.268 ± 0.0011?m, the longestone was 0.746 ± 0.0059 ?m, the shortest of total length chromosomes was 0.27 ± 0.001 ?m, andthe longest of total length chromosomes was 1.41 ± 0.045 ?m. The R value was 5.267 ± 0.15408indicating that radiated ratsnake from Sewon, Bantul, Daerah Istimewa Yogyakarta haschromosome variation size.Keywords: Coelognathus radiatus, blood culture, chromosome character, karyotype
First record of characterization C-KIT mutation in buccal swab DNA of piebaldism suspect balo from balo community, Pujananting South Sulawesi Muthiadin, Cut; Arisuryanti, Tuty; Aziz, Isna Rasdianah; Hajrah, Hajrah; Khatimah, Khusnul; Hidayat, Muhammad Tasri
Tropical Genetics Vol. 4 No. 1 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i1.67

Abstract

ABSTRACT Piebaldism is a disorder in the area of ​​the body caused by an autosomal genetic predominance of the development of melanocytes.The method used in this study is a qualitative method which aims to determine the variation of the KIT-c gene present in the To Balo mucosa samples.The results of this study indicate thatThe change is caused by a point mutation process where in patients with piabeldism the nucleotide bases of sequence 1391 and 1392 undergo changes, where the normal nucleotide base adenine (A) changes to cytosine (C) and cytosine (C) changes to adenine (A). So it can be concluded that the To Balo community in Bulo-Bulo village, Pujananting District, Barru Regency, South Sulawesi suffers from an autosomal dominant hereditary disease or piebaldism.
Cryptic Diversity of Barred Mudskippers, Periophthalmus argentilineatus (Valenciennes, 1837), from the Southern Coast of Java and East Lombok, Indonesia inferred by COI Mitochondrial Gene Arisuryanti, Tuty; Aji, Katon Waskito; Herawati, Happy; Sari, Indah Paramita; Rha’ifa, Febrina Amaliya; Febriyanti, Diana; Priyono, Dwi Sendi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84328

Abstract

The Barred Mudskipper (P. argentilineatus) is an amphibious fish species that displays fully terrestrial behaviour during low tides. Previous studies have indicated the existence of cryptic species of the barred mudskipper, leading to difficulties in taxonomic identification due to similarities in morphological characteristics. Therefore, this study aimed to generate DNA barcodes for Indonesian barred mudskipper populations. We collected ten specimens from Clungup Beach and Kondang Bandung Beach, representing our samples. Additionally, we incorporated 25 previously collected COI sequences from Indonesia into our analysis. The mitochondrial COI gene was amplified using PCR and analysed using various bioinformatics programs. This study provides evidence for the presence of three genetically distinct clades (A, B, and C) within the P. argentilineatus population in Indonesia, with a deep genetic divergence of 2.41% to 6.12%. Clade A showed a high genetic divergence of 5.51-6.12%, suggesting the presence of a cryptic species consistent with previous studies. The high level of haplotype diversity and low nucleotide diversity observed in each clade suggest a population bottleneck followed by a rapid expansion. The lack of geographical separation in the haplotype network analysis indicates that gene flow between populations may have been facilitated by glaciation events in the past. These findings contribute to a better understanding of the biodiversity of the barred mudskipper species in Indonesia and will aid in the accurate identification of cryptic species. This study highlights the importance of using molecular techniques to complement morphological identification in understanding the evolution and diversity of mudskipper fish species. 
KOMPOSISI NUKLEOTIDA GEN MITOKONDRIA D-LOOP IKAN GLODOK (Periophthalmus argentilineatus Valenciennes, 1837) DARI PANTAI BAROS BANTUL, D.I. YOGYAKARTA Arisuryanti, Tuty
Berkala Ilmiah Biologi Vol 15 No 3 (2024)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v15i3.13976

Abstract

Indonesia is a country with a larger area of water than land. Consequently, the diversity of organisms inhabiting Indonesian waters is highly varied. Mangroves are one of the aquatic ecosystems found in Indonesia. One type of fish that can be found in the mangrove ecosystem is the barred mudskipper (Periophthalmus argentilineatus). One of the mangrove forests that Periophthalmus argentilineatus can be found is the Mangrove Forest in Baros Beach, Bantul, Special Region of Yogyakarta. The mangrove ecosystem is influenced by tidal cycles, so the barred mudskipper has unique adaptations, such as breathing with gills during high tide and breathing through the skin and the mucus lining of the mouth and throat during low tide or dry conditions, as well as storing water in its gill cavities. However, research on the composition of mitochondrial D-loop nucleotide of the barred mudskipper is still very limited. The data is crucial and can be used in conservation efforts. Therefore, this study aimed to determine the nucleotide composition of the mitochondrial D-loop gene sequence of the mudskipper (Periophthalmus argentilineatus) collected from Baros Beach, Bantul, Special Region of Yogyakarta. DNA extraction was performed on seven mudskipper samples, followed by DNA amplification using universal primers L15995 and H16498 targeting the mitochondrial D-loop gene. The study results showed variations in the nucleotide composition of T, A, C, and G in the seven mudskipper samples, which were 25.77%, 27.35%, 21.46%, and 25.42%, respectively. The average nucleotide composition of A+T was 47.2%, while G+C was 52.77%.
Genetic Variation of Baram River Frog, Pulchrana baramica (Boettger, 1900), In Java, Sumatra, and Kalimantan based on 16S Mitochondrial Gene Fauzi, Luthfi; Arisuryanti, Tuty; Aji, Katon Waskito; Riyanto, Awal; Smith, Eric N.; Hamidy, Amir
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 4 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84814

Abstract

Baram River Frog (Pulchrana baramica) is a ranid species distributed in the Malay Peninsula, Borneo, Sumatra and represents the sole species from the genus Pulchrana on Java Island. Cryptic species are commonly encountered within the amphibian group which can cause confusion in the identification process. Due to the broad distribution range of P. baramica and the frequent occurrence of cryptic species within the amphibian group, it is important to evaluate the taxonomic status of P. baramica. Therefore, we investigated the taxonomic position of P. baramica from three populations (Kalimantan, Sumatra, and Java) and identified the interpopulation genetic variation based on molecular data of the 16S mitochondrial gene. We reconstructed phylogenetic relationships using Neighbour Joining, Maximum Likelihood, and Bayesian Inference. The research results revealed that Pulchrana baramica is a monophyletic group and nested within a group together with P. glandulosa and P. laterimaculata. The monophyletic group of P. baramica consisted of four distinct lineages that molecularly showed interspecific genetic variation. Clade 1 represents the population of Sumatra and Borneo (Kalimantan), clade 2 comprises the population from Borneo (Sarawak), clade 3 consists of population from Java, and clade 4 represents the population from Sumatra. Further research is required with the addition of morphological and acoustic data as supportive evidence to obtain more extensive comprehension of species identification.
Genetic Diversity of Mudskipper Species (Periophthalmus spp.) from the Southern Coast of Java, Indonesia, Based on the Mitochondrial 16S Gene Arisuryanti, Tuty; Nuraliyah, Tasya; Dwijayanti, Vindi; Aji, Katon Waskito; Priyono, Dwi Sendi; Daryono, Budi Setiadi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1352-1362

Abstract

The genus Periophthalmus (Bloch and Schneider, 1801) comprises a diverse group of mudskippers within the family Oxudercidae and subfamily Oxudercinae. It includes 20 verified species globally, with 11 of these species originating from Indonesia. Among them, P. kalolo and P. argentilineatus are prevalent across seven major islands. However, P. novemradiatus is less documented, with limited distribution records. The morphological similarities among Periophthalmus spp. complicate recognition, often leading to misidentification. To address this challenge, this study investigated the phylogenetic relationships and genetic diversity among these three species, utilizing the 16S rRNA gene as a marker. PCR amplification of the DNA from samples collected from seven regions along Java's southern coast employed primers 16Sar and 16Sbr. Phylogenetic analysis revealed three monophyletic clades corresponding to each species, with genetic divergences ranging from 3.66 to 5.40%. P. argentilineatus displayed a relatively high intraspecific divergence of 0.21-2.26%, suggesting the potential identification of cryptic species within this lineage, even with a conserved gene like 16S rRNA. We identified 47 variable sites within the 552-bp 16S rRNA sequence, including four singleton sites and 43 parsimony-informative sites. We discovered a unique marker at position 172 that could serve as a genetic identifier for distinguishing these species. These findings suggest that the 16S rRNA gene has potential as an alternative marker for species identification while also revealing genetic diversity, thereby complementing or even serving as an alternative to the commonly used COI gene in Periophthalmus spp.
INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS Yusuf, Adib Fakhruddin; Latifah, Vida Rahma; Nurcahyati, Vivi Indah; Nurhasanah, Anggun Diyan; Widyasari, Adristi Shafa; Nainggolan, Ananto Puradi; Hasibuan, Aldy Riau Wansyah; Hidayat, Madyan Akmal; Karmilah, Karmilah; Pratiwi, Arini Dian; Aurantika, Rindu; Hasanah, Muslifah; Aristya, Ganies Riza; Handayani, Niken Satuti Nur; Arisuryanti, Tuty; Lesmana, Indra; Daryono, Budi Setiadi
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2362

Abstract

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.
Co-Authors Aji, Katon Waskito AMIR HAMIDY Atmaja, Vestidhia Yunisya Aurantika, Rindu BUDI SETIADI DARYONO Cut Muthiadin Dwi Sendi Priyono Dwijayanti, Vindi Fauzi, Luthfi Febriyanti, Diana Ganies Riza Aristya Hajrah Hajrah, Hajrah Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Herawati, Happy Hidayat, Madyan Akmal Hidayat, Muhammad Tasri Indra Lesmana Isna Rasdianah Aziz Karmilah, Karmilah Khatimah, Khusnul Latifah, Vida Rahma Matsui, Masafumi Nainggolan, Ananto Puradi Niken Satuti Nur Handayani Nuraliyah, Tasya Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan Pratiwi, Arini Dian Priyono, Dwi Sendi Rha’ifa, Febrina Amaliya Riyanto, Awal Sari, Indah Paramita Smith, Eric N. Smith, Eric N. Tony Febri Qurniawan Widyasari, Adristi Shafa Yusuf, Adib Fakhruddin