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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Medical Journal of Indonesia Current Biochemistry Jurnal Ilmu Ternak Veteriner Warta IHP (Warta Industri Hasil Pertanian) Historika Jurnal Medik Veteriner ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Majalah Patologi Indonesia Indonesian Journal of Applied Research (IJAR) International Journal of Multidisciplinary: Applied Business and Education Research Journal of Education Research Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Journal of Research and Publication Innovation VALUTA Jurnal Administrasi Bisnis, Manajemen dan Ekonomi Policy Brief Pertanian, Kelautan, dan Biosains Tropika Health Gate Jurnal Agripet

Agus Setiyono

Departemen Klinik, Reproduksi Dan Patologi Fakultas Kedokteran Hewan, Institut Pertanian Bogor. Jl. Agatis Kampus IPB Dramaga 16680

Author-ID : 377503

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Seroprevalence of Q fever in sheep and goat in West Java area Setiyono, Agus; Handharyani, Ekowati; Mahatmi, Hapsari
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed globally. Ruminant especially sheep and goats may play an important role in the transmission of the disease to human. The research of seroprevalence of Q fever in sheep and goats was carried out from August 2006 to March 2007 in West Java area. A total of 138 sera were collected; 69 sera from sheep and 69 sera from goats. The indirect immunoflourescent antibody test was used to determine the seroprevalence of Q fever. The seropositive based on the dilution of serum starting from 1 : 16. Seropositive were observed in 22 samples (31.88%) of sheep and 14 samples (20.28%) of goats. The highest titer of 1 : 128 was observed in 3 pregnant sheep. The results of the present study suggested that Q fever was endemic in West Java area. Key Words: Q Fever, Prevalence, Indirect Immunoflourescent Antibody Test

The effect of alpha fetoprotein on NF-ÎºB translocation in lipopolysaccharide induced monocyte-derived dendritic cell Budiyati, Akterono D.; Setiyono, Agus; Tarigan, Elpita; Wibowo, Heri
Medical Journal of Indonesia Vol 21, No 2 (2012): May
Publisher : Faculty of Medicine Universitas Indonesia

Background: Alpha fetoprotein (AFP) is a tumor-associated Ag that has a function in both ontogenic and oncogenic growth and its serum level is elevated in patients with hepatocellular carcinoma (HCC). A recent study showed that the immunoregulatory effect of AFP was through impairment of dendritic cell function as antigen presenting cell (APC), a mechanism that is known to hamper efficient antitumor response. However, the underlying intracellular mechanism of action of AFP required elucidation. As an initial step to determine the signaling pathway of AFP, we analyzed whether LPS induced NF-ÎºB translocation occured in AFP-treated monocyte-derived dendritic cell (MDDC), which was induced by lipopolysaccharide (LPS).Methods: Monocytes were cultured in GM-CSF (800 ng/mL) and IL-4 (1000 ng/mL) containing medium and incubated for six days to generate immature MDDCs with or without the presence of AFP. Mature MDDC was generated by stimulation of the immature MDDC with LPS for another 30 minutes. The analysis of NF-ÎºB translocation was measured by fluorescent microscopy.Results: Following activation of MDDC by LPS, the control group showed a marked nuclear staining of NF-ÎºB. However, the AFP-treated group showed negative nuclear staining similar as observed in unactivated MDDC.Conclusion: This study demonstrated that AFP prevented the activation and nuclear translocation of NF-ÎºB and subsequently might cause the impairment of MDDC function as APC. This finding provides a new insight on the role of AFP in the suppression mechanism of anti tumor immune response. (Med J Indones. 2012;21:97-101)Keywords: Alpha fetoprotein, dendritic cell, lipopolysaccharide, NF-ÎºB translocation

KONDISI HATI TIKUS BETINA AKIBAT INDUKSI 7,12-DIMETHYL BENZ(α)ANTHRASEN (DMBA) DAN PENYEMBUHANNYA DENGAN PROPOLIS DAN NANOPROPOLIS INDONESIA Endang Zainal Hasan, Akhmad; Effend, E. Mulyati; Setiyono, Agus; Sandi, Bayu
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 4, No 1 (2014): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (623.723 KB)

Tujuan dari penelitian ini adalah menentukan efek farmakologis propolis dan nanopropolis untuk pengobatan penyakit hati pada tikus betina yang diinduksi senyawa karsinogenik 7,12 - dimetilbenz(α)antasena (DMBA). Penelitian dilakukan dengan mengamati histopatologi dan makroskopik hati pada 28 ekor tikus betina galur Sprague - Dawley. Tikus percobaan dibagi menjadi 7 kelompok perlakuan dengan 6 kelompok yang diinduksi DMBA (Kelompok I- VI ) dan 1 kelompok sebagai kontrol normal. Kelompok I sebagai kontrolnegatif diberi 1 ml NaCl secara injeksi intraperitoneal (ip). Kelompok II - IV diberinanopropolis 8; 32 dan 56 ppm ip. Kelompok V diberi ekstrak ethanol propolis 233 ppm ip,kelompok VI sebagai kontrol positif diberikan doxorubixin ip dan kelompok VII sebagaikontrol normal diberi penyediaan akuades. DMBA diinduksi selama 11 minggu danpengobatan dilakukan 15 minggu. Setiap minggu tikus ditimbang bobotnya dan diperiksaterhadap inisiasi tumor. Hasil penelitian menunjukkan bahwa ekstrak etanol propolis 233 ppmdan nanopropolis konsentrasi 32 dan 56 ppm dapat mempertahankan kondisi optimal hatitikus. Efeknya adalah setara dengan kontrol normal.

SUPLEMENTASI KELAPA KOPYOR TERHADAP AKTIVITAS SUPEROKSIDA DISMUTASE DAN PATOLOGI ANATOMI HATI TIKUS AKIBAT PERLAKUAN PARASETAMOL Endang Zainal Hasan, Akhmad; Hasim, Hasim; Setiyono, Agus; Winahyu Ariadini, Sekar
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 3, No 1 (2013): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.705 KB)

This research aimed at determining of the effects of the induction of paracetamoland kopyor coconut on the activity of superoxide dismutase (SOD) and pathologicanatomy of rat’s liver. Previous research shows that paracetamol caused negative effect to the body while kopyor coconut has the ability to improve human health, related to its capacity to prevent oxidative damage of human body. In this research, the positive effect of kopyor is approached through measurement of body weight and the activity of the total SOD enzyme of animals tested, which are induced by paracetamol and fed with kopyor. Rat’s administered with temulawak were positive control. The result showed that paracetamol treatment using toxic dose was found to reduce body weight of tested animals. Meanwhile, both kopyor and temulawak supplements were able to counter the negative effect of paracetamol, indicated by the increase in the body weight of tested animals. Other results showed that treatments with temulawak and 5 times-dose of kopyor supplement demonstrated a significantly high total SOD activity in the liver as compared to negative control. Furthermore, pathologic observation of the tested animals’ liver showed that 5 time-dose of kopyor treatment was confirmed to improve the recovery of the liver caused by negative effect of paracetamol, better than the negative control.Key words: kopyor coconut, superoxide dismutase, free radical, pathologic

Ekstrak Teh Hijau Mengurangi Luas Area Fibrosis Paru Tikus Desdiani; Iris Rengganis; Samsuridjal Djauzi; Agus Setiyono; Mohammad Sadikin; Sri Widia A. Jusman; Nurjati Chairani Siregar; Suradi; Putri C. Eyanoer
Majalah Patologi Indonesia Vol 29 No 1 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundPulmonary fibrosis is often found in infectious diseases and chemical exposure in the work environment. Antifibrotic effects can befibropreventive and fibrolysis. Bleomycin can cause inflammation and pulmonary fibrosis. Catechin is proven can repair liverdamages due to alcohol induction. The aim of this study is to examine the effect of antifibrotic green tea catechin on reducing thearea of rat lung fibrotic.MethodsThis research was carried out in vivo experimentally in the Laboratory Animal Management Unit at Faculty of Veterinary MedicineIPB in 4 groups of rat experiments, consisting of 3 rats in the negative control group, 3 rats in the positive control group, 3 rats in thegroup of rats given bleomycin on the 8th day and 28thwith green tea extract every day from day 0 to 49 days (fibropreventive), 3groups of rats given bleomycin days 0 and 21 with green tea extract every day from day 15 to 49 (fibrolysis). Histopathologicalexamination was performe for all groups of rats.ResultsThis study showed that administration of green tea extract in fibropreventive groups and fibrolysis groups could reduce the fibrosisarea in rat lungs based on Aschroft's modified scale.ConclusionBased on the results of this study it was found that the anti-fibrotic activity of green tea extract was proven to be able to suppressthe development of pulmonary fibrosis both by fibropreventive and fibrolysis

PEMBENTKAN KARAKTER KERJA KERAS PADA SISWA MELALUI KEGIATAN HIZBUL WATHAN (Studi Kasus di SMA Muhammadiyah 2 Sukoharjo) Suryanto, Eko; Wiyono, W.; Setiyono, Agus
HISTORIKA Vol 20, No 1 (2017): Pendidikan Sejarah sebagai Pembentuk Karakter
Publisher : Sebelas Maret University (UNS)

This study aims to describe the character's hard work on the formation of students through the activities of the Hizbul Wathan (HW) at SMK Muhammadiyah 2 Sukoharjo. This type of research is a qualitative single case study with strategy. Data sources the study consisted of informants, places and events, as well as documents. Using data collection techniques interviews, observation, documentation, and Forum Group Discussion (FGD). The validity of the data using triangulation techniques, sources, and researchers. Data analysis using interactive models, Miles and Huberman.The results showed that the activities of the Hizbul Wathan in SMK Muhammadiyah 2 Sukoharjo was made for the formation of character means hard work on students. The formation of the character's hard work reflected in the skills that must be mastered students such as line-marching, playing, sports first aid kits, Trommel, spirituality, to the camp. Hard work character's was also established through the promise and law Wizard HW. The promise of Pandu pronounced voluntarily by members when appointed to be a member of the initial commitment is for committing yourself in settling and keeping our promises. While the legislation is a provision that moral Pandu made a habit of behaving yourself in and behave as citizens berakhlaq noble.Â

Potensi Ekstrak Air Angkak dalam Mencegah Peroksidasi Lipid dan Efeknya terhadap Organ Hati Tikus Sprague Dawley Hasim, .; Nathasia, Novena; Setiyono, Agus; Faridah, Didah Nur
Warta Industri Hasil Pertanian Vol 37, No 1 (2020)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/warta ihp.v37i1.5438

Angkak (red yeast rice), bahan pangan fermentasi beras oleh kapang Monascus purpureus dan memiliki beberapa senyawa metabolit bersifat antioksidan.Â Penelitian ini bertujuan menganalisis potensi ekstrak air angkak dalam mencegah peroksidasi lipid di dalam serum dan organ hati tikus.Â Sprague Dawley yang diinduksi propiltiourasil dan diet tinggi lemak. Tikus sebanyak 25 ekor dibagi menjadi lima kelompok [normal, kontrol positif, kontrol negatif, induksi air angkak 50 mg/kg (kelompok A1), dan 100 mg/kg (kelompok A2)]. Parameter uji yang diamati adalah konsentrasi malonaldehid (MDA) serum darah dan hati, aktivitas enzim aminotransferase serum, dan gambaran mikroskopik hati. Tikus dengan induksi air angkak 100 mg/kg menunjukkan penghambatan peningkatan konsentrasi MDA pada serum dan hati serta aktivitas AST signifikan terhadap kelompok kontrol negatif. Histopatologi hati kelompok yang induksi ekstrak air angkak menunjukkan adanya perbaikan jaringan yang secara kualitatif ditandai dengan sel hepatosit utuh yang diwarnai oleh pewarna Hematoksilin-eosin, susunan sel rapat dan berjajar secara radial. Induksi 100 mg/kg masih menunjukkan perbesaran vakuola atau butiran lipid yang berwarna putih yang menjadi penanda degenerasi lemak.

BERAS ANALOG SEBAGAI PANGAN FUNGSIONAL DENGAN INDEKS GLIKEMIK RENDAH Santi Noviasari; Feri Kusnandar; Agus Setiyono; Slamet Budijanto
Jurnal Gizi dan Pangan Vol. 10 No. 3 (2015)
Publisher : Food and Nutrition Society of Indonesia

ABSTRACTThe objective of this research was to produce rice analogue as functional food with low glycemic index. The rice was made from white corn and sorghum with additional soybean flour. The physicochemical properties of rice analogue being analysed were resistant starch content, total phenolic content, dietary fiber, color (L*, +a, +b values) and whiteness (oHue). Rice analogue was potentially used as functional food indicated by high level of resistant starch 2.59%-3.31%, total phenolic content 0.18-0.25 mg GAE/g sample and dietary fiber 5.35%-6.14%. Rice analogue from white corn with and without soybean flour was further tested for glycemic index. Rice analogue from white corn has 69 GI value, while rice analogue from white corn with 10% soybean flour addition has 50 GI value.Keywords: functional food, glycemic index, rice analogueABSTRAKTujuan penelitian ini adalah untuk mendapatkan beras analog yang berpotensi sebagai pangan fungsional dan bernilai indeks glikemik rendah. Beras analog dibuat dari bahan baku jagung putih dan sorgum dengan penambahan tepung kedelai. Sifat fisiko kimia beras analog yang dianalisis adalah pati resisten, total fenol, serat pangan, warna (nilai L*, +a, +b) dan derajat putih (oHue). Beras analog berpotensi sebagai pangan fungsional, yang ditunjukkan dengan tingginya kadar pati resisten yaitu sebanyak 2,59%-3,31%, total fenol sekitar 0,18-0,25 mg GAE/g sampel dan serat pangan antara 5,35%-6,14%. Beras analog berbahan baku jagung putih dengan dan tanpa penambahan kedelai dipilih untuk uji indeks glikemik. Beras analog dari jagung putih memiliki nilai IG 69 sedangkan beras analog dari jagung putih dengan penambahan tepung kedelai 10% memiliki nilai IG 50.Kata kunci: beras analog, indeks glikemik, pangan fungsional

Induksi dan Purifikasi Antibodi Anti-Coxiella burnetii untuk Deteksi Post Mortem Q Fever pada Ruminansia Nina Herlina; Agus Setiyono; Vetnizah Juniantito; Syahruddin Said
Acta VETERINARIA Indonesiana Vol. 7 No. 1 (2019): Januari 2019
Publisher : IPB University

Q fever merupakan penyakit zoonosis yang disebabkan oleh bakteri gram negatif Coxiella burnetii dan terdistribusi luas di seluruh dunia. Gejala klinis pada hewan ternak bersifat tidak konsisten dan tidak spesifik. Metode imunohistokimia telah digunakan untuk mendiagnosis kasus Q fever pada hewan ternak. Antibodi yang digunakan untuk metode imunohistokimia masih tergolong mahal dan harus diimpor dari negara lain sehingga produksi antibodi menjadi peluang. Penelitian ini bertujuan untuk memproduksi antibodi poliklonal anti-C.burnetii dan mengkarakterisasi antibodi tersebut untuk mendeteksi antigen pada organ limpa, paru-paru, dan hati sapi dari Rumah Potong Hewan (RPH) di Medan yang sebelumnya telah dikonfirmasi positif C.burnetii. C. burnetii strain Nine Mile (NM) dan Complete Freund’s Adjuvant (CFA) digunakan untuk menginduksi antibodi pada dua ekor kelinci New Zealand White (NZW) jantan berumur 10-16 minggu. Boosting dilakukan menggunakan immunogen yang sama dengan Incomplete Freund’s Adjuvant (IFA) pada hari ke-14 pascainduksi. Pemanenan serum dilakukan pada hari ke- 24 pascainduksi. Purifikasi serum dilakukan menggunakan dua tahapan, yaitu presipitasi dengan ammonium sulfat saturasi 35% dan filtrasi gel dengan matriks Sephadex G-75. Konsentrasi akhir antibodi yang diperoleh adalah sebesar 11,3 μg μL-1. Sodium Dodecyl Sulfate Poly Acrylamide Gel Electrophoresis (SDS PAGE) dan Western Blot digunakan untuk mengkarakterisasi antibodi yang diproduksi. Selanjutnya dilakukan pewarnaan imunohistokimia menggunakan antibodi tersebut dan sebanyak 90% limpa, 100% paru, dan 100% hati bersifat imunoreaktif yang ditandai dengan warna kecokelatan pada sitoplasma makrofag. Hasil ini menunjukkan bahwa antibodi yang telah dipurifikasi tersebut mampu mendeteksi antigen C. burnetii pada ruminansia.

Performance of Broilers Chicken Due to The Administration of Raw Propolis Akhmad Endang Zainal Hasan; Ike Agustiani; Olive Wisepti Pratama; Syifa Khaerani; Mutholaah; Muhammad Zulkifli; Dimas Andrianto; Agus Setiyono
Indonesian Journal of Applied Research (IJAR) Vol. 1 No. 2 (2020): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v1i2.48

The use of antibiotics as an Antimicrobial Growth Promoter (AGP) for livestock has been banned because antibiotic residues in livestock meat can trigger antibiotic resistance in consumers. Propolis is a natural source that has benefits like antioxidants, antiviral, antifungal, and antibiotic. The purpose of this study was to determine the effectiveness of the addition of feed from the optimal composition of raw propolis to increasing broiler chickens' body weight of broiler chickens. This study used broiler chickens as experimental animals. The chicken were grouped into six groups consisting of two control groups and four treatment groups with different level concentrations of propolis (2, 4, 6, and 8 g/kg feed). After treatment for four weeks, supplementation of raw propolis in the feed had not been able to provide a positive effect on growth performance and protein levels that were not significantly different from controls. Group chickens with propolis additions 6 g/kg feed showed the best results compared to other treatment groups. The group had final weight values, food conversion ratio (FCR), and carcass weight in 1970.67 gram, 1.52, and 1349.83 gram, respectively. The highest percentage of the carcass was shown by group chicken with propolis additions 2 g/kg feed, which is equal to 74.46%. Raw propolis can reduce feed consumption but increase feed conversion ratio.

Co-Authors Aditya Wahyu Nugraha Afifah, Amalia Aisjah Girinda Akhmad Endang Zainal Hasan Akterono D. Budiyati Allaily Allaily Altaff Hendry Alvin Jefry Amrozi - Andriyanto . Anita Esfandiari Arif Yahya Aulia Dina Kristina Bayu Sandi Chintia Ayu Puspita Damar, Ario Daniel Latief Andre Darsono Darsono Desdiani Diah Nugrahani Pristihadi Dian Maulia Utami Dian Pratiwi DIMAS ANDRIANTO Dinar Arifianto Djumali Mangunwidjaja Dwi Astuti E. Mulyati Effend Effend, E. Mulyati Eko Prasetyo Nugroho Eko Suryanto Ekowati Handharyani Elok Puspita Rini Elpita Tarigan Eny Nur Aisyah Evy Damayanthi Fachriyan Hasmi Pasaribu Feri Kusnandar Gani, Ainal Gatut Ashadi Handayu Untari Handayu Untari Hanif Alamudin Manshur Hapsari Mahatmi Hardika Hardika, Hardika Hasim Hasim Hasim Hasim Hasim Hasim Hasim Hasim, . Hirawan Setiadi Husnawati, . I Ketut Suada I Komang Gede Wiryawan I wayan Teguh Wibawan Idqan Fahmi Ike Agustiani IPB, DGB Iris Rengganis Ismah Atika Salmah Japanis Permatasari, Retno Jusman, Sri Widia Azraki Kustiariyah Tarman Lala M Kolopaking Liesbetini Haditjaroko Lily Natalia M Agus Setiadi M. Aman Yaman M. Misbah, M. Ma'mun Sarma Maharani Maharani Maningtyas, Rosyidamyani Twinsari Masdiana C. Padaga MEGA SAFITHRI Miko Afrian Min Rahminiwati Mochamad Alfinanda Santriagung Mohammad Irham Bagus Santoso Mohammad Sadikin Muhammad Daud Muhammad Ihsan Muhammad Luthfi Rahman Muhammad Ridla Muhammad Zuhdi Badruzzaman Muhammad Zulkifli Muharam Saepulloh Mutholaah Nadine Hanifa Permana Nahrowi Nahrowi Nahrowi Nathasia, Novena Neni Nuryani Nina Herlina Noviasari, Santi Nurjati Chairani Siregar Nurliani Bermawie Nurliani Bermawie Oktariza, Wawan Olive Wisepti Pratama Ono Suparno Pribadi, Yohanes Kusuma Purwiyatno Hariyadi Putri C. Eyanoer Rachmat Nabib Rahayu WoroWiranti Rayendra, Raendi Restu Librani Retno Damayanti Soejoedono Risa Tiuria Samsuridjal Djauzi Sandi, Bayu Sangko Sayuti Nasution Sekar Winahyu Ariadini Shila Rahmafia Putri SILMI MARIYA Siti Zulaikhah Slamet Budijanto Sri Purwaningsih, Sri Sri Wahyuni Salam Sudradjat Sumiati Suradi Suria Darma Tarigan Syahruddin Said Syifa Khaerani Teo Qin Yan Titi Candra Sunarti Tomas Iriyanto Tri Isyani Tungga Dewi Trioso Purnawarman Tsulatsy Puspitasari Purnamiharja Ulfah Juniarti Siregar Unang Patriana Upik Kesumawati Hadi Utamimah, Sri Vetnizah Juniantito Win Satya Rudramurti Winahyu Ariadini, Sekar Wiranda G Piliang Wiwin Winarsih Wiyono, W. Yeni Kartika Sari, Yeni Kartika

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