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All Journal Jurnal Pengendalian Hayati Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGROHITA Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika PLAKAT : Jurnal Pelayanan Kepada Masyarakat Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat JPMP Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI)

Yenny WURYANDARI

Fakultas Pertanian Universitas Pembangunan Nasional “Veteran” Jawa Timur

Author-ID : 3514998

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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INTRODUKSI FORMULA PUPUK HAYATI BERBAHAN AKTIF PSEUDOMONAD FLUORESCENT ISOLAT PF-122 UNTUK MENINGKATKAN PERTUMBUHAN DAN PRODUKSI TANAMAN CABAI DI LAPANG Wuryandari, Yenny; Wiyatiningsih, Sri; ., Maroeto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Introduction of biofertilizer containing pseudomonad fluorescent isolate Pf-122 as active ingredient for improving growth and production of chili plant in the field.Some biological control agents required an efficient formulation required to be apply on large scale in the field. The purpose of this research is to review some biofertilizer formulations form of active ingredients pseudomonad fluorescent Pf-122 which can increase growth and production chilli in the field. The research using Randomized Complete Block Design (RCBD) which consists three kinds of formulation. The formulations are powder, granules and pellets. Chilli without formulations as a control. The best results was growth and production of chilli can be showed from plant height, branch number, productive branch number, fruit number, and fruit weight which was given powder formulation. The results of fruit number, the powder formulation which is given can produce double chilli yield than yield from control plant. Moreover, weight fruit from chilli which is given powder formulation can produce fourfold yields. Growth and production chilli which is given others formulations such as granules and pellets were not significantly different results. The overall result of this research, the best formulation to increase growth and production of chilli in the field was formulations contain active biopesticide pseudomonad fluorescent Pf-122 in powder.

Population Dynamics of Pseudomonad fluorescent Isolate Pf-122 In Formula in Different Save Containers Wuryandari, Yenny; Wiyatiningsih, Sri; Suwandi
Nusantara Science and Technology Proceedings 5th International Seminar of Research Month 2020
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0933

The development of biopesticide formulas is carried out by optimizing the use of natural media materials. The formula is made easily, cheaply, and can still maintain the viability of bacteria. This study aims to determine the effect of various formulas and storage containers on the viability of Pseudomonad fluorescent Pf-122. Eight kinds of formulas include; 1) Non-hydrolyzed rice crab at a dose of 400 (K400), 2) at a dose of 200 (K200), 3) Hydrolyzed papaya rice crab at a dose of 400 (KP400), 4) at a dose of 200 (KP200), 5) Pineapple hydrolyzed rice crab with a dose of 400 (KN400), 6). at a dose of 200 (KN200), 7) Media King'S B at a dose of 400 (KB400), 8). at a dose of 200 (KB200). The formula is stored in a plastic and paper storage container. Population dynamics were observed at 1-week intervals. In the paper container, at week 1, the highest bacterial population was in the KP 400 and K400 formulas. At weeks 3 to 8, the population experienced a very rapid decline in all formulas, except for the KN400 formula. In the KN400 formula, at weeks 2 and 3, the population reached 107 CFU / ml and experienced a slow decrease until week 8 (population 101CFU / ml). In plastic containers, at week 1, the highest bacterial population was in the formula KB200, K200, and KP400. At the 3rd to 8th week of observation, the population experienced a rapid decline in all formulas, except for formula K400. In the K400 formula, starting from weeks 2 and 3, the population reached 106 CFU / ml and decreased until the 8th week (population 103CFU / ml). As for the KN400 formula, it can still last until the 7th week with a population of 102 CFU / ml. The longest survival rate of Pf-122 bacteria in the two storage containers was the KN400 formula. The best survival time of Pf-122 bacteria (long-lasting time and high population) is the formula of rice crab with a dose of 400 (K400) in a plastic container.

Daya Tahan Hidup Pseudomonas putida Strain Pf-20 dalam Beberapa Macam Inokulum Yenny Wuryandari; Triwidodo Arwiyanto; Bambang Hadisutrisno; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12211

For any crop-protection agent, an efficient formulation is a necessity to translate laboratory activity into adequate field performance. There are particular challenges to be faced in formulation of biological control agents, because the active ingredient is a living organism that must be kept relatively immobile and inactive while in storage, but quickly resume its normal metabolic processes once applied to the target site. The objective of the research was to study survival of Pseudomonas putida strain Pf-20 in various formulations at the storage time and germination. The twelve formulations include carriers, additives and concentration of Pf-20. The efficacy of various formulation in maintaining the population of Pf-20 in storage was assessed. The research result showed that population of Pf 20 in the formulation number seven was the highest, with the combination peat+talc, CMC+arginin and concentration of Pf[20 10^10 CFU/ml. In peat+talc, CMC+arginin, Pf-20 10^10 CFU/ml based formulation the bacteria survived even up to 84 days of storage although the population declined. In all formulations, population of Pf-20 increased at the time of seed germination. At the time of seed germination, formulation number seven was the highest too.

FORMULA BERBAHAN AKTIF PSEUDOMONAD FLUORESEN DAN PENGARUHNYA TERHADAP PERKEMBANGAN PENYAKIT LAYU PADA CABAI Yenny Wuryandari; Sri Wiyatiningsih; Maroeto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Formula with active ingridient of fluorescent pseudomonads and its influence on wilt disease development of pepper. The purpose of this study was to synergize organic fertilizer with biopesticides of fluorescent pseudomonads 122 to be the best formula to increase the resistance of pepper plants to wilt disease. The study used a completely randomized design (CRD), which consisted of four treatments. The treatments were forms of active ingredient formula of fluorescent pseudomonads (powder, pellet, granule, and liquid). The effectiveness of the formula in suppressing development of the disease was measured by observing the incubation period, index of the disease and discoloration of vascular tissue of pepper. The results showed that the disease incubation period of plant treated with liquid and powder formula was longer than formula with granule and pellet. Plants treated with powder formula showed the lowest disease index compared to control, liquid, pellet and granule formula. Disease index and discoloration in vascular tissue was also of the lowest value when the plants were treated with powder formula. This result indicate that active ingredient of fluorescent pseudomonads formulated in powder was the most effective to inhibit the development of wilt disease on pepper caused by Fusarium sp. and Ralstonia solanacearum.

Kemampuan Antagonistik Beberapa Isolat Pseudomonad Fluoresen Terhadap Bakteri Ralstonia Solanacearum Penyebab Penyakit Layu Pada Tanaman Tomat Yenny WURYANDARI; Arika PURNAWATI; Triwidodo ARWIYANTO; Bambang HADISUTRISNO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.295 KB)

Bacterial Wilt disease (Ralstonia solanacearum) is a main problem in tomato plant.Controls of it have been optimum success yet. Control of it using certainly pseudomonad fluorescentstrain can pressure plant disease developments which cause soil pathogen.The aim of the research, getpseudomonad fluorescent from tomato rizosfer which can inhibit of wilt bacteria disease Ralstoniasolanacearum. Research methods are isolation and identification of pathogen bacteria andantagonistic bacteria. Biological control agents candidate selection was done with antagonistic invitro and inhibit mechanism test. The Research show; there is wilt symptom in tomato areal at WajakMalang. From isolation get Ralstonia solanacearum bacteria with identity white coloni, fluidal,irregular shape in YPGA media and high virulensi. From tomato rizosfer soil isolation in the sameareal get 130 isolate of pseudomonad fluorescent in King’s B media. Antagonistec test in vitro to itisn’t of all bacteria can inhibit Ralstonia solanacearum growing. From them which was tested, getvariation of inhibit zona from 4 mm until more than 30 mm. More of inhibit mechanism isbacteriostatic and only many is bacterisida.

VIABILITAS PSEUDOMONAD Fluorescens ISOLAT Pf-122 DALAM BEBERAPA FORMULA Yenny Wuryandari; Arika Purnawati; Siswanto Siswanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009
Publisher : The East Java Biological Society

The objectives of this research were to examine effect of several formulas on the viability of Pseudomonas Fluorescens isolate Pf- 122. The eight formulations include carriers, additives, and concentration of Pf-122. The efficacy of various formulation in maintaining the population of Pf-122 in storage was assessed. The result of the viability test of Pf-122 in formula showed that the highest population of Pf-122 in week 0 was observed on the formula 1, viz the formula with combination of pupuk kandang as carrier and CMC as additive and Pf-122 at the concentration of 1010 CFU/ml. The bacterial population of the formula number1 could reach 4.8 × 1010 CFU/formula and followed by the formulas number 2,3,4,5,6, and 7. The lowest population was observed on the formula number 8 with the density of 5.8 × 106 CFU/formula. The bacterial population decreased during storage in all of the formulas and even it could not be detectedin the formulas number 3 and 4 after the first week of storage. In the formulas number 1 and 2 survived within 3 months.

PENGARUH BACILLUS SP. TERHADAP MALFORMASI HIFA PATOGENPHYTOPHTHORA PALMIVORA Devi Tria Anjarsari; Endang Triwahyu Prasetyawati; Yenny Wuryandari
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 1 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i1.6810

Busuk Buah kakao adalah salah satu penyakit penting yang disebabkan oleh jamur patogen Phythophthora palmivora. Pengendalian penyakit busuk buah kakao dapat menggunakan agensia hayati Bacillus sp. yang salah satu kandungannya adalah enzim ekstraseluler. Berdasarkan kemampuan yang dimiliki Bacillus sp. maka penelitian ini bertujuan untuk mengetahui pengaruh dari Bacillus sp. dalam mengakibatkan malformasi hifa patogen Phytophthora palmivora. Bakteri Bacillus sp. yang digunakan dalam penelitian ini merupakan bakteri Bacillus sp. koleksi Dra. Endang Triwahyu P., MSi yaitu isolat Ba-6, Ba-9, Ba-12, Ba-15, dan isolat Ba-17. Metode pelaksanaan dalam penelitian ini yaitu pengambilan sampel buah kakao, isolasi dan pengamatan mikroskopis Phytophthora palmivora, peremajaan bakteri Bacillus sp., uji antagonisme, uji aktivitas enzim ekstraseluler Bacillus sp, dan pengamatan hifa patogen Phytophthora palmivora. Enzim ekstraseluler yang diamati yaitu enzim amilase, protease dan selulase. Perlakuan isolat Ba-6 dan Ba-9 menghasikan enzim amilase, protease dan seulase yang mengakibatkan hifa P. palmivora membengkok dan membengkak, isolat Ba-12 menghasilkan enzim protease yang mengakibatkan hifa P. palmivora memendek, membengak, dan terpotong, isolat Ba-15menghasikan enzim amilase, protease dan seulase yang mengakibatkan hifa P. palmivora memendek dan terpotong, isolat Ba-17 menghasikan enzim amilase dan protease yang mengakibatkan hifa P. palmivora membengkak namun jumlah hifa yang membengkak sedikit.

The Influence of Some Types of Biological Agent Formula and the Different Application Time on the Population of the Biological Agent Bacteria and The Chili Plants Yenny Wuryandari; Sri Wiyatiningsih Suwandi
Nusantara Science and Technology Proceedings 4th International Seminar of Research Month
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2019.0446

One alternative for suppressing disease and promoting plant growth is to use the formula for biological agents. The research aims to determine the best formulation of Pseudomonad fluorescent Pf-122 and to determine the right application time in stimulating the growth of chili plants. The formula is made by mixing bacterial active ingredients with semi-natural media of rice crab by hydrolyzing using pineapple and papaya, as well as organic fertilizer. The study used 2 treatment factors. The first factor is the type of formula consisting of control using only Pf-122 (F0), Pf-122 + K (rice crab) (F1), Pf-122 + KN (rice crab by hydrolyzing using pineapple) (F2), Pf-122 + KP (rice crab by hydrolyzing using papaya) (F3). The second factor is application time; watering five days before planting and watering while planting. The results showed that plant growth, namely plant height, number of leaves, root length, and root weight did not differ statistically significantly from all treatment types of application. When viewed from the time of application of the formula, there is also no difference in the growth of chili plants. However, when viewed from the average value, the control without formula tends to show a lower growth value compared to that given the formula. In the generative growth phase, which is when the flowers appear, the chili plants are given a formula faster than without the formula. Application for all types of formulas, the time of occurrence of flowers is not significantly different. The number of Pf-122 populations in the rhizosphere decreases and increases are unstable in all formula applications. The high Pf-122 population in the rhizosphere does not show high chili growth either

Inhibitory Test of Bacillus sp. against Phytophthora palmivora Causes Cocoa Fruit Rot Disease Devi Tria Anjarsari; Endang Triwahyu Prasetyawati; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2003

Fruit rot is one of the important diseases in cocoa fruit, which is caused by Phytophthora palmivora. Farmers often control these pathogen using chemical pesticides. However, this method has a detrimental impact on the environment and health. More environmentally friendly fruit rot disease control can use biocontrol agency. Bacillus sp. is one of the biocontrol agents that is able to produce antibiotics that can inhibit the growth of pathogens, especially soil-borne pathogens and have the ability to colonize plant roots. The purpose of this study is to determine the potential of Bacillus sp. in inhibiting the growth of the pathogenic fungus Phytophthora palmivora in vitro. This study used two experimental factors, type of isolates Bacillus sp. Ba-6 isolate, Ba-9 isolate, Ba-12 isolate, Ba-15 isolate, and Ba-17 isolate and the concentration of Bacillus sp. (106 cfu/ml and 109 cfu/ml). The parameters observed were the inhibitory power and the mechanism of inhibition of Bacillus sp. against Phytophthora palmivora. The results of the best in vitro inhibition test on Ba12K2 treatment with an inhibition of 61% with an inhibitory mechanism, namely antibiosis, as evidenced by the presence of zones of inhibition and swelling of the hyphae.

Pest and Disease Control in Cavendish Banana Seedlings Resulting from Tissue Culture Indah Sari Dwi Agustin; Penta Suryaminarsih; Putranto Sasikirono; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2013

Cavendis banana cultivation can use tissue culture as one of the developments of cultivation in the era of disruption. However, the results of tissue culture are very susceptible to attack by nuisance organisms during acclimatization to plant culture. The control carried out against the attack of plant-disturbing organisms in addition to using fungicides and insecticides, also applies preventive control. The purpose of this study was to determine an effective and smart way to control pests and diseases in tissue cultured Cavendish banana seedlings. Preventive control of pests and diseases using the method of thinning the seeds and soaking the seeds with fungicides. Data analysis was carried out using descriptive and parametric data. This study was conducted using a completely randomized design (CRD) with four control treatments. Each treatment was repeated 4 times. The control treatments carried out consist of: Control (A), preventive control (B), chemical control (C), and a combination of preventive and chemical control (D). The results of the control carried out showed that the combination of preventive and chemical control treatments gave significant results in inhibiting the attack of pests and diseases of Cavendish banana seedlings from tissue culture.

Co-Authors Achmad Fiqri Agus Wahyudi Aisyah Lulu Hariyanto Anjani, Ajeng Distya arfah Arika Purnawati Bambang HADISUTRISNO Bambang Hadisutrisno Budi Rianto Devi Tria Anjarsari Devi Tria Anjarsari Didiek Tranggono Edo Pandu Pratama Fadia Nur Rahma Budiarto Frida Nur Aisah Gita Pawana Hera Amalia Putri Belangi Herry Nirwanto HIDAYATI, KIKI I. Hartana Indah Sari Dwi Agustin Indarwati Indarwati Kaswan Badami Kiki Sandra Amelia Kusuma Wardhani Mas'udah Lestari, Safira Rizka M. Husni Tamrin, M. Husni Maroeto ., Maroeto Maroeto Maroeto Mas’udah, Kusuma Wardhani Mochammad Mirza Saputra Munir, Ahmad Arsyad Nauval Zakqy Noni Rahmadhini Noni Rahmadhini Nova Andriani Novita Endah Ariani Nuriatus Sholikhah Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Prasetyawati, Endang Triwahyu Putranto Sasikirono Putri, Ratih Nadhila Raharja, Wildan Taufik Rahmadhini, Noni Ratu Yusnita Zhalsabilla Rulinggar, Nia Safira Rizka Lestari Safira Rizka Lestari Sagala, Yosua Nathanael Itona Saputra, Mochammad Mirza Setiawan Setiawan Shelomitha Kumala Mawardhany Sherly Arindawati Sri Wiyatiningsih Sulistyanto Sulistyawati, Hanik Suwandi Syamsul Arifin Taufik Hidayat Tr Mujoko Tri MUJOKO Triwidodo ARWIYANTO Triwidodo Arwiyanto Wahyu, Endang Tri Windriyanti, Wiwin Yovita Nathania Yuwono, F. Tsara Nafisah Zainal Abidin Achmad Zakqy, Nauval

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