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Biochemival Characterization of an Antibactrial Glycoprotein from Achatina fulica ferussac Snail Mucus Local Isolate and Their Implication on Bacterial Dental Infection Berniyanti, Titiek; Waskito, Edy Bagus; s, Suwarno
Indonesian Journal of Biotechnology Vol 12, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.074 KB)

Snails crawl over a variety of potentially contaminated surfaces and their foot is the primary site of entry forpathogens, parasites and a range of opportunistic organisms, so it is a little wonder that they must have a defensivesystem to protect them. The mucus secreted on the body surfaces of mollusks is known to play crucial role inlocomotion, feeding, osmoregulation, reproduction and protection of epithelial surfaces. The snail mucus alsocontains Glycoaminoglycans (GAGs) which are complex polysaccharides that participate in the regulation ofphysiological processes through the interactions with a wide variety of proteins. GAGs, such as heparin, serve as keyto biological response modifiers, in example for acting asa a target for pathogen and parasitic factors for attachment,invasion, and immune system.For years, it has been known that the mucus secretions from snails Achatina fulica ferussac local isolate can be usedas a medication, and even empirically it is used to treat infected teeth tahat is suffered by people in rural area. Theantibacterial factor was surveyed in the aqueous extract and the mucin fraction of snail Achatina fulica ferussac, andthey exhibited positive antibacterial for Gram-positive, Escherichia coli and Gram negative, Streptococcus mutans. Inthe following study, it has been proved that an antibacterial content in the mucus was a Glycoprotein. It wascomposed of two subunits of Molecular Weight (MW) 71-73 kDa. The GelCode Glycoprotein Staining Kit detectedglycoprotein sugar moieties in polyacrylamide gel and on nitrocellulose membrane, while the glycoproteincarbohydrate estimation kit detected glycoprotein and estimated carbohydrate content. The glycoprotein contentwas 4.537 ± 0.876 for carbohydrate and 6.420 ± 1.242 for protein.Keywords : characterization, glycoprotein, Achatina fullica Ferussac snail mucus, galur Jawa, antibacterialfactor

Distribution of Snail Mucous Extract (Achatina Fulica) on the Number of Wound’s Basal Epithelial Cells in Rats of Wistar Strain Dina Karimah Putri1 , Titiek Berniyanti 2, Edhi Jularso 3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11611

Background: Acharan sulfate, a new glycosaminoglycan isolated from Achatina fulica, obtained from Kediri,East Java, Indonesia, plays a big role in wound healing process, because it can promote re-epithelialization.Purpose: This study aims to prove that extract of Achatina fulica mucous will increase the number of basalepithelial cells of the wound incision in the back of white male rats of wistar strain. Method: This researchwas done by an in-vivo experimental laboratory, using 14 white male rats of wistar strain that given anincision injury in the back with a length of ± 2 cm and depth of ± 2 mm. They were divided into two treatmentgroups, that are control group which is the incision wounds were given saline solutions; and treatment groupwhich is the incision wounds were given an extract of Achatina fulica mucous with crude acharan sulfatelevel is 86,74%. This treatment is given 3 ml once a day for five days. On the sixth day, biopsy was carriedout to make a histological specimen, then evaluate under a microscope to account the number of basalepithelial cells of the wound incision. Then statistical analysis was conducted by using independent samplest-test with ?=0,05. Results: There were significant differences between two treatment group, that are thenumber of basal epithelial cells in control group and treatment group (p<0,05). Conclusion: Extract ofAchatina fulica mucous with crude acharan sulfate level is 86,74% increase the number of basal epithelialcells of the wound incision in the back of white male rats of wistar strain.

Biochemival Characterization of an Antibactrial Glycoprotein from Achatina fulica ferussac Snail Mucus Local Isolate and Their Implication on Bacterial Dental Infection Titiek Berniyanti; Edy Bagus Waskito; S. Suwarno
Indonesian Journal of Biotechnology Vol 12, No 1 (2007)
Publisher : Universitas Gadjah Mada

Snails crawl over a variety of potentially contaminated surfaces and their foot is the primary site of entry forpathogens, parasites and a range of opportunistic organisms, so it is a little wonder that they must have a defensivesystem to protect them. The mucus secreted on the body surfaces of mollusks is known to play crucial role inlocomotion, feeding, osmoregulation, reproduction and protection of epithelial surfaces. The snail mucus alsocontains Glycoaminoglycans (GAGs) which are complex polysaccharides that participate in the regulation ofphysiological processes through the interactions with a wide variety of proteins. GAGs, such as heparin, serve as keyto biological response modifiers, in example for acting asa a target for pathogen and parasitic factors for attachment,invasion, and immune system.For years, it has been known that the mucus secretions from snails Achatina fulica ferussac local isolate can be usedas a medication, and even empirically it is used to treat infected teeth tahat is suffered by people in rural area. Theantibacterial factor was surveyed in the aqueous extract and the mucin fraction of snail Achatina fulica ferussac, andthey exhibited positive antibacterial for Gram-positive, Escherichia coli and Gram negative, Streptococcus mutans. Inthe following study, it has been proved that an antibacterial content in the mucus was a Glycoprotein. It wascomposed of two subunits of Molecular Weight (MW) 71-73 kDa. The GelCode Glycoprotein Staining Kit detectedglycoprotein sugar moieties in polyacrylamide gel and on nitrocellulose membrane, while the glycoproteincarbohydrate estimation kit detected glycoprotein and estimated carbohydrate content. The glycoprotein contentwas 4.537 ± 0.876 for carbohydrate and 6.420 ± 1.242 for protein.Keywords : characterization, glycoprotein, Achatina fullica Ferussac snail mucus, galur Jawa, antibacterialfactor

Side effects of mercury in dental amalgam Titiek Berniyanti; Ninuk Hariyani
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 1 (2008): March 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Dental amalgam is an alloy composed of mixture of approximately equal parts of elemental liquid mercury and an alloy powder. The popularity of amalgam arises from excellent long term performance, ease of use and low cost. Despite the popularity of dental amalgam as restorative material, there have been concerns regarding the potential adverse health and environmental effects arising from exposure to mercury in amalgam. They have long been believed to be of little significance as contributors to the overall body burden of mercury, because the elemental form of mercury is rapidly consumed in the setting reaction of the restoration. In 1997, 80% of dentist in Indonesia still using amalgam as an alternative material, and 60% of them treat the rest of unused amalgam carelessly. In recent years, the possible environmental and health impact caused by certain routines in dental practice has attracted attention among regulators. As part of point source reduction strategies, the discharge of mercury/amalgam-contaminated wastes has been regulated in a number of countries, even though it has been documented that by adopting appropriate mercury hygiene measures, the impact of amalgam use in dentistry is minimal. The purpose of this paper is to examine on studies that relate mercury levels in human to the presence of dental amalgams. It is concluded that even though mercury used in filling is hazardous, if normal occupational recommendations for proper mercury hygiene routines and source of reduction strategies are followed, no occupational health risk can be assumed.

The effect of gender differences on dentist’s performance (a study in health centers in East Java) Titiek Berniyanti; Kromodiharjo Sudiyono
Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 4 (2007): December 2007
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Gender is a social relationship concept that differentiates role and function between man and women. Equality of rights and obligations between man and woman have been normatively guaranteed and the Constitution does not explicitly discriminate the rights and obligations of woman in law and government, in occupation and decent life, in politics, in religion and beliefs, and in national defend. Related with gender perspective, the need of health worker especially dentist worker has to be determined and planned properly. The productivity is one of the factor on dentist worker, that affect on the quality of health delivary. Some companies try to increase efficiency in performing their activities and try to measure activities they do. In this case, time & motion study method is one of the solutions to help the company measuring their activity. Using these techniques, company can measure the productivity of resources used for every activity. In order to get better performance in cost reduction, the company should assign their cost to the product resulted. Determination of the Standart time to work is one of the method that contain high value of efeciency to gain the productivity. The aim of this study was to learn how gender has an effect on dentist’s work performance. The time of completing dentist’s task i.e: teeth extraction were measured and the standard time were determined. In addition the patient’s respond were measured through questionair. This study was conducted in East Java. The conclusion of this study revealed that there was no difference of work performance between male and female dentist’s.

Apoptosis of Rattus novergicus gingival fibroblasts caused by silver nano-particles gel exposure Kharinna Widowati; Titiek Berniyanti; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 3 (2015): September 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The use of silver nanoparticle are growing, especially in medical science. It’s used in many concentration. In dentistry, it’s used to decrease halitosis, periodontal diseases, and wound healing. It can affect the viability of the cells, give bad effects to the human’s health and environment if used in a long duration and in certain concentration. Purpose: The purpose of this study was to learn the apoptosis of gingival fibroblasts in Rattus novergicus which is exposed with 15 µg/ml silver nano-particle gel by the expression of caspase-3. Method: This study used 9 male wistar rats and were divided into 3 groups. Sample in group A were cut (hurt) in the oral gingiva and exposed to Ag-Np gel 15 µg/ml for 3 days. After 3 days, they were sacrificed and cut the gingival fibroblasts off 3x4 cm size with scalpel. Samples in group B were cut in the oral gingiva and exposed to Ag-Np Gel 15 µg/ml for 5 days. After 5 days they were sacrificed and the gingival fibroblasts off 3 x 4 cm with a scalpel. Samples in group C were cut in the oral gingiva and exposed to none for 3 days then cut the gingival fibroblasts off 3 x 4 cm size with scalpel. The expressions of caspase-3 in the apoptotic and wound healing process were analyzed by Immunohistochemical test. This data was analyzed by using the t-test method. Result: Mean expression numbers of caspase-3 in the group A=5.67; group B=11.33; and group C (control)=18.67. T-test sign.number of group A and C=0.009; group B & C=0.000. Conclusion: The exposure of 15 µg/ml silver gel nanoparticle to gingival fibroblasts of Rattus novergicus reduces the expressions of caspase-3 in the day-3 and day-5 post exposure. The amounts of cell death through the apoptotic pathway which were analyzed by the expressions of caspase-3 will decrease too.

Correlation between working position of dentists and malondialdehyde concentration with musculoskeletal complaints Hari Wibowo; Titiek Berniyanti; Jenny Sunariani
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Musculoskeletal complainst of dentists have become common issues in dentistry since the awkward positions during treating the patients; such as position of a dentist who bends towards the patient, moving abruptly, twisting the body from a side to another side. All those movements are done several times in long term. Such high level of activity without sufficient recovery time may lead to an oxidative stress, so it will affect on the musculoskeletal and concentration of malondialdehyde (MDA) in blood. Purpose: To identify the correlation between malondialdehyde concentration on the risk of musculoskeletal complaint on dentist with working position of maxilla dental patching at community health center(Puskesmas) in Surabaya. Method: The type of research conducted was observational analytics using cross sectional approach. Sampling technique utilized in this research was cluster random sampling techniquewith the total samples of 19. Result: From data analysis result using Spearman correlation test, it was found that there was a significant correlation between working position and musculoskeletal complaint using Spearman correlation test and the p value obtained was<0.05. This research also found that there was a significant correlation between malondialdehyde concentration and musculoskeletal complaint using Pearson correlation test and the p value obtained was <0.05. Conclusion: There is a significant correlation between working positions of dentists and there is a significant correlation between working position and malondialdehydeconcentration with the musculoskeletal complaints.

Correlation between working positions and lactic acid levels with musculoskeletal complaints among dentists Fiory Dioptis Putriwijaya; Titiek Berniyanti; Indeswati Diyatri
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 4 (2016): December 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Musculoskeletal complaints have been common for dentists since their body is unknowingly often in inappropriate positions when caring for patients. For example, they bend towards patients, suddenly move, and then rotate from one side to another. The repetitive movements are done in long term. High activities and sufficient recovery time can cause a buildup of lactic acid in their blood leading to obstruction of the energy intake from the aerobic system in their muscle cells, resulting in fatigue. As a result, such conditions trigger decreased muscle performances. Purpose: This study aimed to determine the correlation between working positions and lactic acid levels with the risk of musculoskeletal disorders among dentists at Public Health Centers in Surabaya. Method: This research was an analytical observational research using cross sectional approach. Sampling technique used in this research was cluster random sampling with nineteen samples. To evaluate the working positions of those samples, a rapid entire body assessment (REBA) method was used. Meanwhile, to observe the musculoskeletal disorders of those samples, a Nordic body map was used. Data obtained then were analyzed using Pearson correlation test with a significance level (p<0.05). Result: Results of data analysis using the Pearson correlation test showed that the significance value obtained was 0.036. It indicates that there was a correlation between the working positions and the lactic acid levels with the musculoskeletal disorders in those dentists. The results of the Pearson correlation test also revealed that there was a correlation between the working positions and the lactic acid levels among those dentists with a significance value of 0.025. Conclusion: It may be concluded that the wrong body positions during working can increase lactic acid level in the body of dentists. The increased level of lactic acid then can affect their muscles, leading to the high risk of musculoskeletal disorders.

The correlation between the use of personal protective equipment and level wild-type p53 of dental technicians in Surabaya Puspa Dila Rohmaniar; Titiek Berniyanti; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Exposure of metals among dental technicians that come from the working environment can lead to the formation reactive oxygen species (ROS). ROS can cause mutations in the p53 gene (p53). The mutation is transversion mutation GuanineThymine. p53 mutations can lead to low expression of the wild-type p53 protein (p53). Wild-type p53 involved in many biological processes such as regulation of genes involved in cell cycle, cell growth after DNA damage, and apoptosis. However, exposure to metals among dental technicians can be prevented through the use of personal protective equipment (PPE) during work. Purpose: The purpose of this study was to analyze the correlation between the use of personal protective equipment to wild-type p53 protein levels among dental technicians in Surabaya. Method: This study was observational analytic with cross sectional approach. 40 samples were taken by random sampling. Data were retrieved through interviews and observations. Wild-type p53 was analyzed from saliva with indirect ELISA method. Analysis of data used Kolmogorov Smirnov normality test and a Pearson correlation test. Value significance was p<0.05 (95% confidence level). Result: There was a significant association between the use of personal protective equipment with wild-type p53 levels with p=0.002 Conclusion: The use PPE properly is positively correlated with the wild-type p53 protein levels of dental technicians in Surabaya.

PENINGKATAN KETAHANAN MASYARAKAT BENJENG TERHADAP DAMPAK BENCANA BANJIR Abu Bakar; Ninuk Dian Kurniawati; Makhfudli Makhfudli; Titiek Berniyanti; Budi Utomo
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 2 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Public participation in flood risk reduction needs to be undertaken in the overall flood disaster management cycle, from the start of preparedness, the period before the flood, the period during the flood and the period after the flood disaster. The purpose of Community Service is to improve the community’s ability to overcome the impact of flood disaster. The results of community service in Benjeng Sub District on Thursday, October 19, 2017 has been well underway. This is seen from the public interest to follow very high activity, as evidenced by the attendance of participants following the activities reached 100%. The outcomes of the activities are: Draft Articles Scientific publications in the journal Pengmas, Understanding and skills of the people of Benjeng Sub-district of Gresik Regency in overcoming the impact of floods are found to increase, and the health of the people of Benjeng sub-district of Gresik regency can minimize the loss due to verbal flooding.AbstrakPartisipasi masyarakat dalam pengurangan risiko banjir perlu dilakukan dalam siklus manajemen bencana banjir secara menyeluruh, dari mulai kesiapsiagaan, masa sebelum bencana banjir, masa selama banjir dan masa setelah bencana banjir. Tujuan Pengabdian pada Masyarakat yaitu meningkatkan kemampuan masyarakat dalam mengatasi dampak bencana banjir. Hasil yang dilakukan dari pengabdian pada masyarakat yang ada di Kecamatan Benjeng pada hari Kamis, tanggal 19 Oktober 2017 telah berlangsung dengan baik. Hal ini terlihat dari animo Masyarakat untuk mengikuti kegiatan sangat tinggi, terbukti dengan kehadiran peserta mengikuti kegiatan mencapai 100%. Luaran kegiatan yang dicapai diantaranya: Draft Artikel Publikasi ilmiah di jurnal Pengmas, Pemahaman dan keterampilan masyarakat Kecamatan Benjeng Kabupaten Gresik dalam mengatasi dampak banjir ditemukan peningkatan, dan ketenteraman/kesehatan masyarakat kecamatan Benjeng Kabupaten Gresik dapat meminimalkan kerugian akibat banjir secara verbal.

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