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STUDI PERTUMBUHAN BEBERAPA ISOLAT JAMUR TIRAM (Pleurotus spp.) PADA BERBAGAI MEDIA BERLIGNIN , Achmad; Nina Herliyana, Elis; , Wartaka
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 11, No 3 (2008): November 2008
Publisher : Jurnal Pengkajian dan Pengembangan Teknologi Pertanian

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Abstract

The Growth Study of Some Oyster Mushroom (Pleurotus spp.) Isolates on Some Ligneous Media. Research to study the growth of some pleurotus isolates on some ligneous media were conducted at Forest Pathology Laboratory, Faculty of Forestry and Biological Science Study Center, Bogor Agricultural University in September 2004 to March 2005. Substances which used were Pleurotus sp.l, Pleurotus sp.6, and Pleurotus sp.8 from Forest Pathology laboratory collection, PDA, MEA, MPA, some natural lignin source to be added to the commercial media. Optimum media for Pleurotus sp.1 is PDA, MEA + bamboo apus dust, and Glenn and Gold modification + sengon wood dust. Pleurotus sp.6 grow best at MPA, MEA + paddy straw dust, and Glenn and Gold modification + paddy straw dust. Optimum media for Pleurotus sp.8 is MPA, MBA + paddy straw dust added media, MEA + paddy hay dust, and Glenn and Gold modification + sengon wood dust. The difference of colony growth is caused by isolate and nutrition of each growth media. Pleurotus sp.6 and Pleurotus sp.8 known produce lyses zone at media which contain lignin source. Lyses zone caused by existence of extrasellular enzyme which secreted by mushroom hype to degrade lignin. All mycellium dry weight of Pleurotus spp. isolat that is given wobble is higher than dont given. Mycellium dry weight from high to low showed by Pleurotus sp.8, Pleurotus sp.6 and Pleurotus sp.l. The difference of colony growth caused by isolat and nutrition of each growth media. Lysis zone at media with lignin source caused by extracellular enzyme activity to degradate lignin source as their nutrition. The difference of mycellium dry weight at both treatment is caused by the response to oxygen in the liquid media. Key words: Pleurotus spp, PDA, MEA, MPA, bamboo dust, sengon wood dust, Glenn & Gold, modification. Penelitian ini bertujuan untuk mempelajari pertumbuhan koloni beberapa isolat jamur tiram (Pleurotus spp.) yang dikulturkan pada berbagai media dengan sumber lignin alami, dilakukan dan bulan September 2004 sampai Maret 2005, bertempat di Laboratorium Penyakit Hutan, Fakultas Kehutanan, dan Laboratorium Mikrobiologi dan Biokimia, Pusat Studi Ilmu Hayat, Institut Pertanian Bogor. Bahan yang digunakan adalah Pleurotus sp.1, Pleurotus sp.6, dan Pleurotus sp.8, MEA, MPA, PDA, dan beberapa macam sumber lignin alami yang ditambahkan pada tiga media komersial tersebut. Pleurotus sp.l tumbuh terbaik pada media PDA, MEA + serbuk kayu sengon, serta media modifikasi Glenn dan Gold + serbuk bambu apus. Pleurotus sp.6 tumbuh terbaik pada media MPA, MEA + serbuk jerami padi, serta media modifikasi Glenn dan Gold + serbuk jerami padi. Isolat Pleurotus sp.8 tumbuh terbaik pada media MPA, MEA + serbuk kayu sengon, serta media modifikasi Glenn dan Gold + serbuk jerami padi. Pleurotus sp.6 dan Pleurotus sp.8 menghasilkan zona lisis berbentuk lingkaran coklat kekuningan pada media yang ditambah sumber lignin alami. Bobot kering miselia Pleurotus spp. pada media malt ekstrak cair yang ditambah serbuk jerami padi atau serbuk kayu sengon dengan diberi penggoyangan, lebih tinggi dibanding dengan Pleurotus spp. pada media yang sama tanpa diberi penggoyangan. Bobot kering miselia tertinggi sampai terendah berturut-turut ditunjukkan oleh Pleurotus sp.8, Pleurotus sp.6, dan Pleurotus sp.1 . Kata kunci: Pleurotus spp, PDA, MEA, MPA, serbuk bambu, serbuk kayu sengon, modifikasi Glenn dan Gold
Root Diseases Ganoderma sp. on the Sengon in West Java and East Java Elis Nina Herliyana; Darmono Taniwiryono; Hayati Minarsih
Jurnal Manajemen Hutan Tropika Vol. 18 No. 2 (2012)
Publisher : Institut Pertanian Bogor (IPB University)

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Abstract

Sengon tree (Paraserianthes falcataria (L.) Nielsen) currently becomes a major forest tree species widely planted by smallholders in Indonesia. The wood of this is quick growing and relatively easy to sell. However, level of plant safety sengon between crop plantations and other forestry need to be assessed considering the sengon tree is alternative host of Ganoderma spp. Studies have been conducted to know the presence and diversity of Ganoderma spp. on the sengon tree and some ways inoculation on sengon plant in the nursery. Survey of Ganoderma conducted in several locations of community forests and cacao (Theobroma cacao) plantations in West Java and East Java. Testing of genetic diversity based on RAPD technique. This conducted at the Biotechnology Research Institute of Plantation Indonesia Bogor. Inoculation testing conducted at the Faculty of Forestry, Bogor Agricultural University. The results showed that Ganoderma lucidum was found on the sengon tree and cacao plant, generally on the dead stump. The test results of genetic diversity obtained genetic similarity between G. lucidum from sengon and cacao are quite close. The results of inoculation of G. lucidum testing on seedlings sengon showed that both isolate from cacao and sengon tree able to infect a sengon tree back. The existence of sengon tree as shade plants for cacao plant need to watch out, because production cycle of sengon tree faster than production cycle of cacao plant that is protected.
Pengendalian Serangan Ganoderma spp. (60-80%) pada Tanaman Sengon sebagai Pelindung Tanaman Kopi dan Kakao Elis Nina Herliyana; Darmono Taniwiryono; Hayati Minarsih; Muhammad Alam Firmansyah; Benyamin Dendang
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Information about genetic variation of Ganoderma spp. As a couse of rot disease on plantation crops is necessary for consideration in efforts to protect crops. Exploration of the use of biological agents, especially Trichoderma spp., for the control of Ganoderma on forestry crops is still limited to laboratory testing. Its effectiveness to control Trichoderma spp. To protect plants in the nursery sengon being carried out, as well as to deternime its role in improving plant growth.
Bakteri Endofit dari Tanaman Kehutanan sebagai Pemacu Pertumbuhan Tanaman Tomat dan Agens Pengendali Meloidogyne sp. Abdul Munif; Arif Rafi Wibowo; Elis Nina Herliyana
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.311 KB) | DOI: 10.14692/jfi.11.6.179

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Meloidogyne sp. is one of the main constraints of tomato production in Indonesia. Endophytic bacteria may be considered as biocontrol agents for controlling Meloidogyne sp. The objective of this study was to isolate endophytic bacteria from forestry plants and to evaluate its potential for controling Meloidogyne sp. on tomato. Endophytic bacteria were isolated from roots of mahoni (Swietenia mahogany), trambesi (Albizia saman), gaharu (Aquilaria malacensis), and meranti (Shorea sp.). Isolation of bacterial endophytes from plant tissue was conducted using surface sterilization method with 70% alcohol, 3% NaOCl and sterile water on medium trypsic soy agar. Endophytic bacteria was separated and purified based on shape and color of the colony. A total of 33 isolates of endophytic bacteria were isolated from roots of mahoni (11 isolates), trambesi (5 isolates), gaharu (7 isolates), and meranti (10 isolates). The bacteria was tested for the hypersensitivity reaction on tobacco plants and the result showed that 22 isolates did not cause necrosis, indicated they are not pathogenic. Ten isolates of endophytic bacteria was selected for further experiment, i.e. to evaluate their potential as biocontrol agent for Meloidogyne sp. and as growth promotor for tomato plants. The experiment was conducted in the screenhouse using seed treatment. The result showed that two isolates of endophytic bacteria, i.e. MSJ1H and AGS1F were able to increase the growth of tomato plants up to 60% and reduce the number of root knot caused by Meloidogyne sp. Endophytic bacteria isolated from forestry plants have the potential as a biocontrol agents to plant parasitic nematode Meloidogyne sp. 
Morphological Feature Extraction of Jabon’s Leaf Seedling Pathogen using Microscopic Image Melly Br Bangun; Yeni Herdiyeni; Elis Nina Herliyana
TELKOMNIKA (Telecommunication Computing Electronics and Control) Vol 14, No 1: March 2016
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/telkomnika.v14i1.2486

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This research aims to analyze morphological techniques for feature extraction of Jabon’s leaf seedling pathogen using digital microscopic image. The kinds of the pathogen were Curvularia sp., Colletotrichum sp., and Fusarium sp.. Pathogens or causes of disease were identified manually based on macroscopic and microscopic observation of morphological characters. Morphological characters describe the characteristics of shape, color and size of a pathogen structure. We focused on shape feature by using the morphological techniques to feature extraction. The morphology features extraction used were area, perimeter, convex area, convex perimeter, compactness, solidity, convexity, and roundness. The methodologies were acquisition, preprocessing, features extraction and data analysis for derivative features. With features extraction, we got the pattern that described each pathogen for pathogen identification. From the experimental result showed that compactness and roundness feature were able to differentiate each pathogen due to that the characteristics of each pathogen class were separated.
Leaf Morphological Feature Extraction of Digital Image Anthocephalus Cadamba Fuzy Yustika Manik; Yeni Herdiyeni; Elis Nina Herliyana
TELKOMNIKA (Telecommunication Computing Electronics and Control) Vol 14, No 2: June 2016
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/telkomnika.v14i2.2675

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This research implemented an image feature extraction method using morphological techniques. The goal of this proccess is detecting objects that exist in the image. The image is converted into a grayscale image format. Then, grayscale image is processed with tresholding method to get initial segmentation. Furthermore, image from segmentation results are calculated using morphological methods to find the mapping of the original features into the new features. This process is done to get better class separation. Research conducted on two Antocephalus cadamba (Jabon) leaf diseased seedlings data set image that contained leaf spot disease and leaf blight. The results obtained morphological features such as rectangularity, roundness, compactness, solidity, convexity, elongation, and eccentricity able to represent the characteristic shape of the symptoms of the disease. All properties form the symptoms can be quantitatively explained by the features form. So it can be used to represent type of symptoms of two diseases in Antocephalus cadamba (Jabon).
Feature extraction of Jabon (Anthocephalus sp) leaf disease using discrete wavelet transform Felliks Feiters Tampinongkol; Yeni Herdiyeni; Elis Nina Herliyana
TELKOMNIKA (Telecommunication Computing Electronics and Control) Vol 18, No 2: April 2020
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/telkomnika.v18i2.10714

Abstract

Jabon (Anthocephalus cadamba (Roxb.) Miq) is one type of forest plants that have very rapid growth until the process of the harvest. One inhibitor is a disease that attacks the leaves in the form of spots and blight that can cause death during the growth process of this tree. The purpose of this process is to detect the object of diseases that attack the leaves of jabon at the time in the nursery. Images of affected jabon leaf disease segmented by reducing the RGB color cylinders to separate the disease object from the background. Reduced channel G-R provides information in the form of disease areas contained in the image of Jabon leaf. Furthermore, the characteristics of leaf disease can be detected well using DWT in the 3-level decomposition process with SVM classification results that can separate both classes of spots and blight by 84.672%.
Investigation of mold and blue stain fungi on rattan was conducted at the rattan industry, Cileungsi, Bogor on February 2005. The isolation and identification of fungi on rattan samples was done at the laboratory of Forest Pathology, Faculty of Forestry, Bogor Agricultural University, Indonesia. Rattan samples are cutted in size each 2 cm.  Samples were growth and incubated on medium PDA (Potato Dextrose Agar) for 7 days in 29°C. There are two treatment, surface sterilized is used alcohol 70 % a Elis Nina HERLIYANA
Jurnal Ilmu dan Teknologi Hasil Hutan Vol. 2 No. 1 (2009): Jurnal Ilmu Teknologi Hasil Hutan
Publisher : Departemen Hasil Hutan, IPB

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Investigation of mold and blue stain fungi on rattan was conducted at the rattan industry, Cileungsi, Bogor on February 2005. The isolation and identification of fungi on rattan samples was done at the laboratory of Forest Pathology, Faculty of Forestry, Bogor Agricultural University, Indonesia. Rattan samples are cutted in size each 2 cm.  Samples were growth and incubated on medium PDA (Potato Dextrose Agar) for 7 days in 29°C. There are two treatment, surface sterilized is used alcohol 70 % and non surface sterilized. Each treatment was replicated 4 times.  The identification of fungi was done manually using identification book Barnett (1986). The results showed that fungi dominantly formed from sterilized  samples of rattan were Monilia sp. (55%) and Rhizophus sp. (32.5%). Whereas from non sterilized samples were Rhizophus sp. (54.77%) and than Monilia sp. (30.95%).   Keywords :  Rattan, Monilia sp., Rhizophus sp., mold fungi, blue stain fungi.  
POTENSI Trichoderma sp. dan Gliocladium sp. SEBAGAI JAMUR ANTAGONIS TERHADAP Cylindrocladium sp. PENYEBAB PENYAKIT LODOH PADA PERSEMAIAN SECARA IN-VITRO Rezeka Amalia; Elis Nina Herliyana; Illa Anggraeni
Jurnal Penelitian Hutan Tanaman Vol 5, No 1 (2008): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3585.84 KB) | DOI: 10.20886/jpht.2008.5.1.63-75

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Salah satu penyakit yang umum menyerang tanaman di persemaian adalah penyakit lodoh (damping off) yang disebabkan oleh jamur    patogen Cylindrocladium sp. Pengendalian biologi dengan menggunakan jamur antagonis Trichoderma sp. dan Gliocladium  sp. merupakan altematif yang diharapkan dapat mengurangi resiko pencemaran dengan meminimalkan gangguan terhadap keseimbangan biologis. Penelitian ini bertujuan untuk mengetahui potensi jamur antagonis Trichoderma sp. dan Gliocladium sp. dalam pengendalian hayati terhadap patogen Cylindroc/adium sp. penyebab penyakit  lodoh pada persemaian tanaman hutan secara in-vitro.Pengujian dilakukan di dalam cawan petri dengan metode biakan ganda (Dual Culture) menggunakan media PDA (agar kentang). Rancangan yang digunakan adalah Split Plot dengan lima perlakuan yaitu perlakuan A untuk satu koloni jamur patogenik Cylindrocladium sp. dengan satu koloni jamur antagonis Trichoderma sp (CT 1:1), B untuk satu koloni jamur patogenik Cylindrocladium sp. dengan dua koloni jamur antagonis Trichoderma sp. (CT 1:2), C untuk satu koloni jamur patogenik Cylindrocladium sp. dengan satu koloni jamur antagonis Gliocladiumsp. (CG 1 :1 ), D untuk satu koloni jamur patogenik Cylindrocladium sp. dengan dua koloni jamur antagonis Giocladium sp. (CG 1:2) dan E untuk Cylindrocladium sp. (kontrol), setiap perlakuan diulang sebanyak lima kali. Parameter yang diukur   adalah pertumbuhan diameter koloni Cylindrocladium  sp. dan persentase penghambatan jamur antagonis terhadap Cylindrocladium sp. Hasil penelitian menunjukkan bahwa  persentase  penghambatan jamur antagonis Trichoderma  sp. terhadap patogen Cylindrocladium sp.  pada perlakuan A (CT 1 : 1) dan perlakuan B (CT 1:2) hari ke-6 masing-masing  sebesar   24,2% dan  22,4%, sedangkan persentase penghambatan pada jamur antagonis Gliocladium  sp. terhadap patogen Cylindrocladium sp. pada perlakuan  C (CG 1:1) dan perlakuan D (CG  1:2)  hari  ke-6  masing-masing  sebesar  19,3%  dan 15,2%. Penghambatan ini disebabkan oleh adanya aktivitas  antibiosis dan lisis serta persaingan  tumbuh dari Trichoderma sp. dan Gliocladium  sp. terhadap Cylindrocladium  sp. 
KARAKTERISTIK FISIOLOGI ISOLAT Pleurotus spp. ACHMAD ACHMAD; ELIS NINA HERLIYANA; OSICA ASNO FERINA YURTI; ANANG PRANOTO HIDAYAT
Jurnal Penelitian Tanaman Industri Vol 15, No 1 (2009): Maret 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n1.2009.46-51

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ABSTRAKStudi in vitro tentang karakteristik fungi isolat Pleurotus spp. telahdilaksanakan di Bogor dari bulan Juli sampai Agustus 2004. Penelitianmenggunakan rancangan faktorial dalam rancangan acak lengkap danbertujuan untuk mempelajari pengaruh media, temperatur inkubasi dan pHmedia terhadap 6 isolat Pleurotus sp. Karakter lain yang juga dipelajariadalah kemampuan untuk mengoksidasi asam tanat dan asam galat dalammedia agar. Hasil penelitian menunjukkan bahwa Pleurotus isolat sp.6dan sp.8 tumbuh baik pada media MPA, isolat Pleurotus sp.1, 3 dan 4pada media MEA dan isolate sp.2 pada media PDA. Kecuali isolat sp.8,isolat lainnya tidak dapat tumbuh pada temperatur 10 dan 35 o C. Pertum-buhan isolat sp.8 terbaik dibandingkan isolat lainnya pada semuatemperatur. Diameter koloni isolat lainnya hanya mencapai 0,2 – 2,33 cm.Pertumbuhan isolat sp.8 juga terbaik pada semua pH media diikuti isolatsp.6 kemudian isolat sp.4. Semua isolat menunjukkan reaksi oksidasipositif pada agar asam tanat dan asam galat yang ditunjukkan oleh warnacoklat pada media yang melingkari koloni.Kata kunci : Pleurotus spp, media, temperature, pH, oksidasi, asam tanat,asam galatABSTRACTPhysiological Characteristics of Pleurotus spp. IsolatesPhysiological characteristics of some Pleurotus sp. isolates werestudied in vitro, from July until August 2004 in Bogor. Experiments tostudy the effect of kind of media, temperature of incubation room, and pHof medium on six isolates of Pleurotus sp. were arranged in factorialrandomized complete design and replicated three times with colony in apetri dish as experimental units. Another physiological character studiedwas the ability to oxidize tannic and gallic acids in agar medium. Resultsshowed that isolates Pleurotus sp.6 and -8 grew better in MPA medium,Pleurotus sp.1, -3, and -4 in MEA, and Pleurotus sp.2 in PDA. ExceptPleurotus sp.8, other isolates could not grow in incubation roomtemperature of 10 and 35 o  C. The growth of Pleurotus sp.8 was the bestamong the isolates in all temperature levels. Other isolates grew poorly in20 and 29o C with diameter range was 0.2 – 2.33 cm. The growth ofPleurotus sp.8 was also the best in all pH medium levels, followed byPleurotus sp.6, and then Pleurotus sp.4. All isolates showed positiveoxidative reaction on tannic and gallic acid agar indicated by brown colorof the medium around the colony.Key words: Pleurotus spp., medium, temperature, pH, oxidation, tannicacid, gallic acid
Co-Authors Abdul Muhyi ABDUL MUNIF Abdul Munif Abdurachman Syafiih Achmad Achmad . Achmad . Achmad . Achmad ; Achmad Achmad ACHMAD ACHMAD Achmad Lisdar Adisti Permatasari Putri Hartoyo Agus Setiawan Ai Rosah Aisah Akhir, Jumadil ANANG PRANOTO HIDAYAT Ananta Kusuma Amanda Andi Sukendro Andrea Ajeng Eirenne Kristianti Anisa Tri Harjanti Ardiansyah Putra Ariana Ariana Arief B. Witarto Arief Noor Rachmadiyanto Arif Ravi Wibowo Arzyana Sunkar Benyamin Dendang Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Deasy Putri Permatasari Dewi Sukma Dodi Nandika Dwierra Evvyernie Dyah LINGGA NP Erianto Indra Putra Eti Artiningsih Octaviani Fatin Hanifah Felliks Tampinongkol, Felliks Fifit Kulsum Fitri Kurniawati Fuzy Yustika Manik Gustan Pari Hanifah Nuryani Lioe Hayati Minarsih Hayati Minarsih Hidayatullah, Deden I. Sudirman Iga Dwi Syahrani Illa Anggraeni Indra Putra, Erianto Irfan Jelata, Tedi Irfan Kemal Putra Irma Badarina Iskandar Z Siregar Isroi Isroi Ivan Permana Putra Kultsum, Fifit Kunio Tsunoda Labana Hutagalung Laila Fithri Maryam Libranika Dwi Miswati Liza Sakbani Lufthi Rusniarsyah, Lufthi Lul Godi, Rizal Lutfi Hanafi Melly Br Bangun Mira Febrianti Muhammad Alam Firmansyah Nabawiah, Safira Nifa Hanifa Noor Rachmadiyanto, Arief Nurulhaq, Muhammad Iqbal OSICA ASNO FERINA YURTI Ratna Jamilah Reny Meisetyani Reza Pamunca, Airres Rezeka Amalia Rizki Nugraheni Amaliasuci Rossy Nurhasanah Safira Nabawiah Santiyo Wibowo Sarah Diana Yulianti Shodiq Syifaudin, Ikhwan Silviana Arsyad Soetrisno Hadi Sri Listiyowati Sri Wahyuni, Devi Sri Wilarso Budi R Surono Suryo Wiyono Syafitri Hidayati Tiara Antika Tjahja Muhandri Toto Toharmat TW DARMONO DARMONO Wartaka Wartaka ; Wasrin Syafii Yurico Bakhri Yusuf Sudo Hadi