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All Journal Jurnal Akuakultur Indonesia Jurnal Akuatika Jurnal Perikanan Kelautan Jurnal Sosial Ekonomi Pesisir Jurnal Harpodon Borneo Jurnal Matematika & Sains Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Biodjati Omni-Akuatika Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Jurnal Biosense International Journal of Research in Community Services Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Farmers: Journal of Community Services Jurnal Perikanan
Yuniar Mulyani
Department Of Fisheries, Faculty Of Fisheries And Marine Sciences, Universitas Padjadjaran Jl. Raya Bandung-Sumedang KM 21 Jatinangor, Sumedang, West Java 45363 Indonesia
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Penanda Molekul DNA Mikrosatelit untuk Karakterisasi Bibit Jamur Kuping (Auricularia polytricha [Mont.] Sacc.) I Nyoman Pugeg Aryantha; Yuniar Mulyani; Rahmat Ariffudin
Jurnal Matematika & Sains Vol 13, No 1 (2008)
Publisher : Institut Teknologi Bandung

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Abstract

This study aims to find out a method of spawn characterization for wood ear mushroom (A. polytricha [Mont.] Sacc.)based on microsatellite DNA marker. Four strains of wood ear mushroom cultivated in several regions of Java i.e AUC(West Java), AUCN (Central Java), AUP (West Java) and AUT (West Java) were evaluated. A pair of designed primers(5’ -GGGAAAGTGATCCCATCTT-3’ and 3’-AGTTGTGGGAACATCGAACT-5’) was able to amplify microsatelliteDNA from those four strains of wood ear mushroom. Strains AUC, AUCN and AUP share the same positions (2 loci) ofmicrosatellite motive (TC)n with PCR products of 212 and 201 bp, while the same motive for strain AUT was found atdifferent loci with PCR products of 127, 108, and 96 bp. The same motive was also found at another locus of strainAUC and AUP with a PCR product of 228 bp. Another different locus with a PCR product of 85 bp also gave positiveamplification result for strain AUC. The data on macroscopic characters (mycelial growth rate and quality, fruitingpercentage and mushroom yield) revealed that AUC, AUCN and AUP are good strains, while AUT is bad strain.Overall, there is a good correlation between molecular (microsatellite DNA) patterns and macroscopic data on mycelialgrowth rate and quality, as well as flushing percentage and mushroom yield. Therefore, microsatellite DNA motive of(TC)n can be adopted for spawn characterization of wood ear mushroom.
BIOPROSPEK AKTIVITAS METABOLIT SEKUNDER DARI LAMUN (Seagrass) DAN BEBERAPA JENIS MAKROALGA (Seaweeds) SEBAGAI KANDIDAT AGEN ANTIBAKTERI PATOGEN Vibrio harveyi YANG MENYERANG UDANG WINDU (Penaeus monodon) (Studi in-vitro) Kurnia Agung, Mochamad Untung; Mulyani, Yuniar; Riyantini, Indah
Jurnal Harpodon Borneo Vol 5, No 1 (2012): Volume 5 No 1 April 2012
Publisher : Jurnal Harpodon Borneo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.202 KB) | DOI: 10.35334/harpodon.v5i1.78

Abstract

Bacterial deseases are seriousness problem in aquaculture. The uncontrolled of quality degradation of pond sites because of wastes and organic matters decompotition and also global climate changes has been predicted affect triggered the bacterial infections. Vibrio harveyi is one of patogenic bacteria which againts Tiger shrimp (Penaeus monodon). The recovery strategies has been applied both in the rehabilitation of pond sanitary and also it has been focussed in curative methods to overcome infection activities. The usage of synthetic antibiotics has been reported gift any negative effects, either for environment and rised pathogenic resintance because of uncontrolled exposure.Environmental friendly and based on biological methods has been promoted to overcome this problem. The usage of antibacterial agents derived from natural compounds  has been recommended as an effective method. Several marine resources has been predicted as candidates of antibacterial agents againts Vibrio harveyi. Several seaweeds (macroalga) and seagrass has been explored in this research at in-vitro level of study to inhibit the activity of Vibrio harveyi. The result showed that all of the crude extracts derived from seagrass (Thallasia sp) and several kinds of seaweeds  (Padina sp, Gracilaria sp and Sargassum sp) could inhibit the growth of Vibrio harveyi from the lowest concentration level (10 µg/mL). The highest inhibition activity has been showed from the antibacterial sensitivity test of crude extract of Thallasia sp at the concentration level of 10.000 µg/mL.                                                                                                                                  Keywords : macroalgae, seagrass, secondary metabolite, antibacteria, Vibrio harveyi
BIOPROSPEK AKTIVITAS METABOLIT SEKUNDER DARI LAMUN (Seagrass) DAN BEBERAPA JENIS MAKROALGA (Seaweeds) SEBAGAI KANDIDAT AGEN ANTIBAKTERI PATOGEN Vibrio harveyi YANG MENYERANG UDANG WINDU (Penaeus monodon) (Studi in-vitro) Mochamad Untung Kurnia Agung; Yuniar Mulyani; Indah Riyantini
Jurnal Harpodon Borneo Vol 5, No 1 (2012): Volume 5 No 1 April 2012
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v5i1.78

Abstract

Bacterial deseases are seriousness problem in aquaculture. The uncontrolled of quality degradation of pond sites because of wastes and organic matters decompotition and also global climate changes has been predicted affect triggered the bacterial infections. Vibrio harveyi is one of patogenic bacteria which againts Tiger shrimp (Penaeus monodon). The recovery strategies has been applied both in the rehabilitation of pond sanitary and also it has been focussed in curative methods to overcome infection activities. The usage of synthetic antibiotics has been reported gift any negative effects, either for environment and rised pathogenic resintance because of uncontrolled exposure.Environmental friendly and based on biological methods has been promoted to overcome this problem. The usage of antibacterial agents derived from natural compounds  has been recommended as an effective method. Several marine resources has been predicted as candidates of antibacterial agents againts Vibrio harveyi. Several seaweeds (macroalga) and seagrass has been explored in this research at in-vitro level of study to inhibit the activity of Vibrio harveyi. The result showed that all of the crude extracts derived from seagrass (Thallasia sp) and several kinds of seaweeds  (Padina sp, Gracilaria sp and Sargassum sp) could inhibit the growth of Vibrio harveyi from the lowest concentration level (10 µg/mL). The highest inhibition activity has been showed from the antibacterial sensitivity test of crude extract of Thallasia sp at the concentration level of 10.000 µg/mL.                                                                                                                                  Keywords : macroalgae, seagrass, secondary metabolite, antibacteria, Vibrio harveyi
Molecular Identification of Bacillus Derived from the Common Carp Intestine and Their Antibiotics Resistance Yuniar Mulyani; Karimah Syakirotin; Kiki Haetami; Yeni Mulyani; Evi Liviawaty; Mochamad Untung Kurnia Agung
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5263

Abstract

The use of bacteria as a biological control agent in aquaculture is such new hope overcoming the negative impacts of the impropriate use of antibiotics. Bacillus is widely known as a potential probiotic for aquaculture, especially which are derived from aquatic biota. Due to a concern that the role of Bacillus as a probiotic agent will be disrupted by residues of antibiotics in the water, this research aimed to identify Bacillus species from common carp intestine based on the molecular method and investigate their resistance to some com-mercial antibiotics that widely used in aquaculture. Chloramphenicol, ampicillin, and metronidazole were used to determine the Bacillus resistance to antibiotics using the Kirby Bauer method. The resistance assay was carried out with a bacterial density of 108 CFU/mL. The results showed that Bacillus isolates derived from common carp  intestine were closely related and resistant to antibiotics. Isolates that resistant to the three types of antibiotics were Cc.1.9 (CgN9) and Cc.2.18 (CgM18).
Effectiveness of Lactic Acid Bacteria to Improve Cyprinus carpio Fingerlings Resistance Against Rosidah Rosidah; Yuniar Mulyani; Walim Lili; Khasanatur Rosyidah
Jurnal Biodjati Vol 5, No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.5260

Abstract

Common carp (Cyprinus carpio) cultivation is often hampered by a disease attack, one of them is the attack of Edwarsiella tarda. Lactic acid bacteria (LAB) can be used as an alternative to prevent diseases in fish by increasing the body's resistance. This research aimed to determine the most effective isolates of LAB that increase of the resistance of carp fingerlings to the attack of E. tarda bacteria and see which isolates can produce the highest survival. The LAB isolates used were the result of isolation from the gut of carp. This study used a Completely Randomized Design (CRD) consisting of four treatments with three replications. The fish were immersed with different LAB isolates, CcB7, CcB8, and CcB15 in the same density of 108 cells / mm3. Immersion was carried out for 30 minutes with a frequency of seven days. While during the research, two immersions were carried out before the challenge test against E. tarda bacteria. The parameters observed were the number of leukocytes, hematocrit, erythrocyte, differential leukocytes, survival rate, and clinical symptoms that appeared. The results showed that all LAB isolates used in this study could increase the body resistance of carp against the attacks of E. tarda bacteria. The LAB CcB7 isolate was the most effective for enhancing the body resistance of carp fish withthe highest increase level of  leukocyte, erythrocyte, and hematocrit were 18 ± 0.057,7 ± 0,077, and 0.26± 7.31% respectively. After being challenged with E. tarda bacteria producing mild clinical symptoms, the highest increase is in monocyte and neutrophil cells was 20 and 62% respectively, the highest reduction in lymphocytes was – 9%  and the highest survival rate was 80%. 
Genetic Relationship of the Transgenic and Non-Transgenic Mutiara Catfish with their Hybrids Sangkuriang Catfish Iskarimah Yolanisa; Ibnu Dwi Buwono; Yuniar Mulyani; Iskandar Iskandar
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4741

Abstract

The male broodstock of Transgenic Mutiara catfish (P) (containing Clarias gariepinus Growth Hormone, CgGH) is a fish who show fast growth. Propagation of the Transgenic Mutiara catfish F1 and F2 progeny involves the crossing of broodstock P with Non Transgenic Mutiara catfish. New genetic variations (polymorphisms) can be generated from crosses broodstock P and F1 which lead to the fast growth inheritance of transgenic fish. Phylogenetic relationship analysis showed distance and genetic variation in broodstock P, F1, F2 and Sangkuriang catfish (as controls) using the RAPD-PCR method (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) as the research purposes. The results showed that OPA-03 (5'-AGTCAGCCAC-3 ') primer was selective for determining phylogenetic among catfish samples. The closest genetic similarity index originates from the female broodstock of Non Transgenic Mutiara catfish (P) (93%), while the farthest originates from the male broodstock of Mutiara Transgenic catfish (P) (51%) with F1 dan F2. Closely related broodstock catfish is not recommended to be crossed.
Study of Symbiont Bacteria of Acropora digitifera Coral From Ciletuh Bay, Sukabumi by Using Culture and Molecular Approach Nurfitri Nurfitri; Fiddy Semba Prasetiya; Indah Riyantini; Yuniar Mulyani; Sulastri Arsad; Mochamad Untung Kurnia Agung
Journal Omni-Akuatika Vol 15, No 2 (2019): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2019.15.2.748

Abstract

Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. These microorganisms help corals in secreting mucus layers which are used as a place to live for bacteria and control the presence of pathogenic bacteria in corals. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in Ciletuh bay, West Java, by culture approach. Sampling was carried out at a depth of 4 meters. Morphology-based identification and molecular approach based on sequence of the small ribosomal unit (16S) rRNA gene were used to determine the bacterial species in the samples. A total of six pure isolates were identified based on morphological observation and the molecular DNA characteristics from three of them were identified with 16S rRNA gene sequences. The identification using 16S rRNA gene showed that the isolate ACD.P4.PH7.P had a close relationship with the BF strain and zb strain of Bacillus flexus (acc number MH569560.1) with a similarity of 85.44 %. While ACD.P4.PH9.P isolate has a close relationship with Bacillus sp. c234 (acc number FJ950647.1) with a similarity of 98.50 %. Additionally, ACD.P4.PH9.K isolates closely related to Bacillus sp. of strain 6RM1 (acc number MK134607.1) with a similarity of 94.78 %. This study also revealed that both microscopic identifications by morphological traits and molecular approach using 16S rRNA gene can be used in bacterial diversity assessment.
PERBANDINGAN BEBERAPA METODE ISOLASI DNA UNTUK DETEKSI DINI KOI HERPES VIRUS (KHV) PADA IKAN MAS (Cyprinus carpio L.) Yuniar Mulyani; Agus Purwanto; Isni Nurruhwati
Jurnal Akuatika Vol 2, No 1 (2011): Jurnal Akuatika
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.051 KB)

Abstract

Penelitian ini telah dilakukan di Laboratorium Bioteknologi Perikanan, Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran. Penelitian ini bertujuan untuk mengetahui efektifitas dalam memperoleh konsentrasi dan kemurnian DNA serta efisiensi waktu pengerjaan dari metode isolasi DNA dengan membandingkan beberapa metode isolasi DNA diantaranya ektraksi DNA dengan Kit (Promega), CTAB dengan phenol, modifikasi CTAB, dan ekstraksi DNA dengan thermal lysis. Parameter yang digunakan adalah nilai kemurnian dan kualitas DNA hasil isolasi yang diperoleh dari analisis spektrofotometri dan analisis elektroforesis serta efisiensi waktu pengerjaan. Sampel yang digunakan adalah jaringan insang dan sirip ikan mas. Konsentrasi DNA dan kemurniannya diukur dengan metode spektrofotometri, sedangkan untuk visualisasi DNA hasil isolasi menggunakan metode elektroforesis serta pengujian keberadaan KHV dideteksi dengan bantuan PCR dengan menggunakan Primer pendeteksi KHV. Penelitian ini menggunakan metode survei dengan analisis deskriptif kualitatif. Hasil penelitian secara umum menunjukkan bahwa metode modifikasi CTAB memberikan hasil isolasi DNA dengan konsentrasi yang tertinggi yaitu 70,10 μg/ml dengan nilai kemurnian berkisar antara 1,9-2,0. Namun waktu yang dibutuhkan untuk pengerjaannya cukup lama. Metode ekstraksi DNA dengan thermal lysis memiliki waktu pengerjaan yang singkat. Namun konsentrasi DNA yang diperoleh cukup rendah yaitu 9,25 μg/ml dengan nilai kemurnian berkisar antara 1,6-1,8.
Hubungan Filogenetik Molekuler Beberapa Jenis Mangrove di Pulau Penjarangan, Ujung Kulon, Provinsi Banten Indah Riyantini; Yuniar Mulyani; Mochamad Untung K. Agung
Jurnal Akuatika Vol 5, No 1 (2014): Jurnal Akuatika
Publisher : Fakultas Perikanan dan Ilmu Kelautan

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Abstract

Penelitian ini bertujuan untuk untuk menganalisis hubungan filogenetik secara molekular dari Beberapa Jenis Mangrove yang terdapat di P. Penjarangan Kawasan Ujung Kulon, yang merupakan kawasan konservasi. Pengambilan sampel dilakukan di beberapa titik di P. Penjarangan dan analisis molekular dilakukan di laboratorium Bioteknologi Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran. Metode penelitian merupakan metode survey, dan dianalisis secara deskriptif kualitatif di laboratorium. Sampel daun mangrove diisolasi DNA, dan diamplifikasi dengan PCR-RAPD menggunakan 10 primer acak. DNA hasil PCR dielektroforesis pada gel agarosa 1,4% dengan menggunakan marker DNA Lambda yang telah dipotong dengan enzim EcoRI dan Hind III. Hasil elektroforesis menunjukkan pola larik yang  diterjemahkan ke dalam bentuk data numerik (1/0) dan dianalisis hubungan filogenetik dan keragaman genetiknya menggunakan program NTSYS-pc.  Hasil penelitian menunjukkan bahwa Metode RAPD menggunakan primer OPA 2 dan OPA 11 dapat menghasilkan polimorfisme pada DNA genom dari beberapa jenis mangrove di Pulau Penjarangan, Kawasan Ujung Kulon. Keanekaragaman genetik pada beberapa jenis mangrove cukup tinggi, dan analisis hubungan filogenetik molekular beberapa jenis mangrove yang ada di kawasan tersebut memiliki hubungan yang tidak berbeda dengan klasifikasi morfologinya.
IDENTIFIKASI BAKTERI INDIGENOUS PEREDUKSI LOGAM BERAT Cr (VI) DENGAN METODE MOLEKULER DI SUNGAI CIKIJING RANCAEKEK, JAWA BARAT Syafrudin Lewaru; Indah Riyantini; Yuniarti Mulyani
Jurnal Perikanan Kelautan Vol 3, No 4 (2012)
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran

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Abstract

Penelitian ini dilakukan untuk mencari bakteri indegenous dari sungai yang berpotensi mereduksi logam berat Cr (VI) dengan metode molekuler . Sampel berasal dari air dan sedimen Sungai Cikijing, Rancaekek Kabupaten Bandung Jawa Barat. Metode penelitian menggunakan analisis deskriptif, identifikasi molekuler dengan primer 16S rRNA, dan uji reduksi bakteri terhadap Cr (VI) dengan indikator DiphenylCarbazide (DPC). Tahapan penelitian meliputi kultivasi bakteri, uji tantang dengan konsentrasi yang digunakan yaitu 50, 150, 500, 1000, uji reduksi dengan konsentrasi 300, 700, dan 1000 ppm, dan identifikasi molekuler gen 16S rRNA. Hasil penelitian didapat 10 Isolat murni bakteri, dan semua bakteri mempunyai resistensi terhadap konsentrasi Cr (VI). Dua bakteri yang mempunyai tingkat resistensi, tingkat reduksi tertinggi dengan melihat OD (optical density), yaitu A.3.3. yang dapat menurunkan 1083,25 ppm menjadi 468,30 ppm dan  S.3.14dapat menurunkan 1083,25 ppm menjadi 856,20 ppm dalam waktu ±24 jam. Hasil identifikasi molekuler menunjukkan spesies bakteri A.3.3 adalah Bacillus thuringiensis dan isolat S.3.14 adalah Staphylococcus arlettae.
Co-Authors Afifah Shabirah Agus Purwanto Aisyah Aisyah . Aisyah Aisyah Aisyah Aisyah Aisyah Aisyah Aldhi Rahmadianto Alin Shelina Nurashila Anqy Riyanto Chitra Devi Amelia Dachlan, Dara Tasya Zallika Davinci, Leonardo Dermawan, Rifai Dian Yuni Pratiwi Eddy Afrianto Elvin Giantara Muharam Eulis Reni Sundari Evi Liviawaty Fiddy Semba Prasetiya Fittrie Mellianawaty Pratiwy Ghina Ilmia Hafshah Grandiosa Herman, Roffi Handaka, Asep Agus Henhen Suherman Herman, Roffi Grandiosa I Nyoman Pugeg Aryantha Ibnu Dwi Buwono Iis Rostini Imtiyaz Nur Imtiyaz Nur Shadrina Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Ine Maulina Ine Maulina Iskandar Iskandar Iskandar Iskandar Iskarimah Yolanisa Isni Nurruhwati Iwang Gumilar Juli A Sinaga Karimah Syakirotin Karsih, Okta Rizal Kharima, Zhafira Putri Khasanatur Rosyidah Kiki Haetami Kiki Haetami Kurnia Agung, Mochamad Untung Luthfi Nur Aulia Mochamad Untung K. Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Muhammad Luthfi Ramadhan Nono Carsono Nur , Imtiyaz Nur Aulia, Luthfi Nurfitri Nurfitri Petrus Putra Bagaskhara Putri Indah Ayuningrum Putri, Miftahul Agra Rahmat Ariffudin Rita Rostika Rizka Nurfadillah Roffi Grandiosa Roffi Grandiosa Herman Rosidah - Rosidah Rosidah Rosidah Rosidah Rudy Kurniadi Rusky Intan Pratama Shadrina, Imtiyaz Nur Sulastri Arsad Sundari, Eulis Reni Syafrudin Lewaru Syakir, Muhammad Sabiq Tiaraningsih, Thania Ujang Subhan Walim Lili Yeni Mulyani Yolanisa, Iskarimah Yuli Andriani Zahidah - Zuzy Anna