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KAJIAN AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA EKSTRAK BIJI, KULIT BUAH , BATANG DAN DAUN TANAMAN JARAK PAGAR (Jatropha curcas L.) Setyaningsih, Dwi; Nurmilah, Ovi Yulianti; Windarwati, Sri
REKAPANGAN Vol 4, No 2 (2010): REKAPANGAN
Publisher : UPN VETERAN JAWA TIMUR

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Abstract

Jatropha  curcas is  one  of  potential  bioenergy  plant.  Jatropha  curcas  also  has  great potential  for  the  development  of  bio-products,  medicines  and  consumer  good  since  it contains some bioactive agents such as β-sitosterol, stigmasterol, curcin, flavonoid and 12-deoxyl-16-hydroxyphorbol  (phorbol  esther)  that  potentials  as  antioxidant  and antimicrobial  agent.  The  purpose  of  this  research  were  to  determine  the  yield  of extract, antimicrobial and antioxidant activity from seed, fruit shell, stem and leaves of  J. curcas. Seed, fruit shell, stem and blend of stem and leaves were extracted with three type  of  solvents.  They  were  methanol,  ethyl  acetate  and  n-hexane.  From  the  yield  of extract,  it  could  be  concluded  that  seed  of J.curcas L  contained  a  lot  of  semi  polar compounds  (35,98%)  and  non  polar compounds  (32,27%),  blend  of  stem  and  leaves contained a lot of polar compounds (9,75%) and J. curcas fruit shell contained a lot of polar  compounds  (5,96%).  Antioxidant  activity  with  scavenging  effect  on  DPPH radical  (1,1-diphenyl-2-picrylhidrazil)  and  antimicrobial  activity  using  well  diffusion method  showed  that  methanol  extract  from J.  curcas seed  had  the  highest  value  of antioxidant  and  antimicrobial  activity  than  other  samples.  The  antioxidant  activity  of methanol  extract  from J. curcas seed  was  93,40%,  comparable  with  ascorbic  acid  as reference. The potency of antimicrobial activity of this extract  could be seen from the bacterial inhibition zone diameter of 11.9 mm for E. coli and 14.83 mm for S. aureus.Keywords: Antimicrobial, antioxidant, Jatropha curcas L
MODIFICATION OF NATURAL ZEOLITE AS MOLECULAR SIEVE MATERIAL ON BIOETHANOL DEHYDRATION Khaidir, Khaidir; Setyaningsih, Dwi; Haerudin, Hery
Jurnal Zeolit Indonesia Vol 8, No 2 (2009)
Publisher : Jurnal Zeolit Indonesia

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Abstract

This study aimed to find a method of modification zeolite structure so it has a fit pore size to used on bioethanol dehydration process and acquiring bioethanol quality for fuel. The method used by heating the mixture of ethanol azeotropic and water at distillation flask which passed through on the column filled by zeolite molecular sieve. The result showed that zeolite modifications (ZMS) have a better characteristic for used on bioethanol dehydration process than natural zeolite. The result which obtained on dehydration process using ZMS was better rather than commercial zeolite 3 A and natural zeolite. Concentration of bioethanol obtained by using ZMS has increased 3,14%.
Sintesis Mono-Diasilgliserol Berbasis Gliserol dan Palm Fatty Acid Distillate Rumondang, Irma; Setyaningsih, Dwi; Hermanda, Atika
Jurnal Kimia dan Kemasan Vol. 38 No. 1 April 2016
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.934 KB) | DOI: 10.24817/jkk.v38i1.1968

Abstract

Mono-diasilgliserol (M-DAG) adalah jenis emulsifier yang banyak digunakan dalam industri pangan maupun non- pangan. M-DAG dapat dihasilkan dari proses esterifikasi. Pada penelitian ini bahan baku yang digunakan yaitu gliserol hasil samping industri biodiesel dan palm fatty acid distillate (PFAD) hasil samping industri minyak goreng. Penelitian ini bertujuan menentukan suhu dan rasio molar antara gliserol dan PFAD yang terbaik dalam menghasilkan M-DAG. Variasi yang digunakan adalah variasi suhu sebesar 150°C dan 160°C serta variasi rasio molar gliserol : PFAD sebesar 1:2, 1:3 dan 1:4. Karakterisasi yang dilakukan meliputi uji warna, komposisi M-DAG dengan kromatografi lapis tipis, stabilitas emulsi, pH dan titik leleh. Hasil penelitian menunjukkan bahwa pada suhu 150°C menghasilkan rendemen sebesar 74%. Sedangkan pada suhu 160°C didapatkan nilai stabilitas emulsi mencapai 72% dengan kadar ALB 39%. Pada rasio molar 1:2 menghasilkan tingkat kecerahan M-DAG sebesar 82.57. 
HIDROLISIS RUMPUT LAUT EUCHEMA COTTONII MENGGUNAKAN ASAM SULFAT DAN KULTUR INAKTIF UNTUK PRODUKSI PREBIOTIK Permatasari, Vitta Rizky; Setyaningsih, Dwi; Haditjaroko, Liesbetini
Jurnal Teknologi Pertanian Vol 19, No 2 (2018)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

ABSTRAKProduksi rumput laut di Indonesia pada tahun 2016 meningkat hampir 4 kali lipat dari tahun 2010. Nilai ini menunjukkan potensi rumput laut yang sangat besar. Jenis yang paling sering dimanfaatkan yaitu Eucheumma cottonii sebagai penghasil karagenan, yang berpotensi sebagai prebiotik karena mengandung galakto-oligosakarida. Tujuan dari penelitian ini yaitu mendapatkan perlakuan lanjut terhadap hidrolisat agar mendapatkan aktivitas prebiotik yang paling baik serta mengkarakterisasi sifat prebiotik dari hidrolisat. Tahapan penelitian meliputi pre hidrolisis, hidrolisis dengan campuran asam sulfat dan kultur inaktif, perlakuan lanjutan dengan 3 jenis (penyaringan, pengendapan, dan tanpa perlakuan), karakterisasi kimia dan mikrobiologis serta pengujian kadar galaktosa dengan HPLC. Hasil dari penelitian ketiga sampel jenis perlakuan, ternyata tanpa perlakuan mempunyai hasil yang paling baik dibandingkan kedua perlakuan yang lain, dengan nilai kadar gula reduksi sebesar 0.37%, total gula 2.88%, pH 4.95, total asam 0.15%, total BAL 62 × 107 CFU/ml, kadar galaktosa dan GOS masing-masing sebesar 0.14% dan 1.18% ABSTRACTIndonesia’s seaweed production in 2016 increased almost 4-fold from 2010. This value indicates a great potential of the seaweed. The most widely used type is Eucheuma cottonii which utilized in carrageenan production. In addition, E. cottonii has potential as a prebiotic due to the galacto-oligosaccharides component. The purpose of this sudy was to determined the best types of treatment of hydrolisate for reached probiotic activity and characterized probiotic content of hydolisate. The preparation stage consists of pre-hydrolysis, hydrolysis using sulfuric acid and inactive culture, and followed by three different types of treatment (filtration, precipitation and without any treatment). The analytical stage consists of chemical and microbiological characterization, measured galactose using HPLC. The results showed that untreated samples had the best results compared with the other two treatments,  with reduction sugar content 0.37%, total sugar 2.88%, pH 4.95%, total acid 0.15%, total BAL 62 × 107 CFU/ml, galactose and GOS content are 0.14% and 1.18%
EVALUASI MINYAK DAUN CENGKEH DAN MINYAK SEREH WANGI SEBAGAI BIOADITIF BAHAN BAKAR SOLAR DALAM MENURUNKAN EMISI GAS BUANG PADA MESIN DIESEL Lawang, Andi Tenri; Setyaningsih, Dwi; Syahbana, Meika
Jurnal Teknologi Pertanian Vol 20, No 2 (2019)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.896 KB) | DOI: 10.21776/ub.jtp.2019.020.02.3

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ABSTRAK Bioaditif bahan bakar solar berbasis minyak atsiri merupakan bahan yang ditambahkan pada bahan bakar agar terbakar sempurna. Komponen oksigen yang terkandung di dalam struktur kimia minyak atsiri serta sifat minyak atsiri yang larut dalam bahan bakar solar diharapkan dapat menyempurnakan sistem pembakaran di dalam mesin. Tujuan penelitian ini adalah mengkaji peranan dari komposisi minyak atsiri yaitu minyak daun cengkeh dan minyak sereh wangi dalam menurunkan emisi yang dihasilkan dari mesin diesel. Bahan bioaditif yang digunakan dalam penelitian adalah minyak daun cengkeh dan minyak sereh wangi serta senyawa eugenol dan kariofilen yang terkandung pada minyak daun cengkeh dan senyawa rhodinol dan sitronellal yang terkandung pada minyak sereh wangi. Pengujian emisi menggunakan alat Bacharach RN1012. Hasil pengujian emisi penambahan bioaditif minyak daun cengkeh menurunkan kadar CO 30%, NO 36%, SO 12% dan total partikulat 30%, Eugenol menurunkan kadar CO 37%, NO 38%, SO 32% dan total partikulat 40%, Kariofilen menurunkan kadar CO 24%, NO 19%, SO  33% dan total partikulat 16%, sedangkan minyak sereh wangi menurunkan kadar CO 23%, NO 31%, SO 22% dan total partikulat 33%. rhodinol menurunkan menurunkan kadar CO 7%, NO 20%, SO 30% dan total partikulat 45%, sitronellal menurunkan kadar CO 38%, NO 37%, SO 2% dan total partikulat 28% terhadap solar yang tidak ditambahkan bioaditif. ABSTRACT Bioaditive from essential oil is an ingredient added to improve the fuel combustion process into the engine. Essentials oil contain chemical component of oxygenated hydrocarbons and  that can dissolve in diesel  fuel. Bioaditive materials used in this research are cleove leaf oil, citronella oil, eugenol and cariophyllene are compounds from clove leaf oil and also rhodinol and critronellal contained in citronella oil. Emission testing is used by Autologic Gas Analyzer Bacharach RN1012. Exhaust emission test given a good result for clove leaf oil reduced 30% CO, 36% NO, 12% SO, and 30% total particulate content,  Eugenol decreases  37% CO, 38% NO, 32% SO and 40% total particulate content , Kariofilen decreases 24% CO, 19% NO,  33% SO and 16% total particulate content. while citronella oil reduces 23% CO, 31% NO, 22% SO and 33% total particulate content. rhodinol decreases 7% CO , 20% NO, 30% SO and 45% total particulate content,  citronellal decreases 38% CO, 37% NO, 2% SO and 28%total particulate for diesel  is not added bioaditive. 
KARAKTERISTIK KEFIR DENGAN PENAMBAHAN PUREE UMBI GEMBILI Kartika, Kartika; Rahayuningsih, Mulyorini; Setyaningsih, Dwi
EDUFORTECH Vol 4, No 2
Publisher : Program Studi Pendidikan Teknologi Agroindustri, UPI (Universitas Pendidikan Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/edufortech.v4i2.19372

Abstract

Kefir merupakan produk susu yang beraroma asam, alkoholik, karbonat, dan dibuat melalui proses fermentasi menggunakan mikroba bakteria dan yeast. Kefir merupakan probiotik alami yang mengandung bakteri hidup yang dapat memberikan efek menyehatkan bagi tubuh manusia apabila dikonsumsi. Umbi gembili mengandung inulin yang dapat dimanfaatkan sebagai prebiotik. Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan puree gembili dan waktu fermentasi pada nilai pH, total asam tertitrasi, total gula dan gula pereduksi, serta pertumbuhan bakteri asam laktat dan khamir pada kefir. Metode yang digunakan dalam penelitian ini adalah eksperimen dengan analisis data deskriptif. Hasil dari penelitian ini menunjukan bahwa penambahan puree gembili sebanyak 4% dapat menurunkan nilai pH, meningkatkan total asam tertitrasi, meningkatkan total gula dan gula pereduksi, serta meningkatkan pertumbuhan bakteri asam laktat dan khamir dibandingkan kefir kontrol. Lama waktu fermentasi kefir puree gembili terbaik adalah selama 24 jam dengan nilai pH 4,17; total asam tertitrasi 0,64%; bakteri asam laktat 8,991 log CFU/ml dan khamir 8,579 log CFU/ml.
PRODUKSI BUBUK SINBIOTIK DARI HIDROLISATEucheuma cottonii MENGGUNAKANSPRAY DRYING Dwi Setyaningsih; Desi Musdaniaty; Neli Muna
Jurnal Teknologi Industri Pertanian Vol. 29 No. 3 (2019): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24961/j.tek.ind.pert.2019.29.3.233

Abstract

Eucheuma cottonii is red algae that contain high carbohydrates, especially carrageenan. Carrageenan is a sulfated polygalactan with 15-40% of ester-sulfate content. It is formed by alternate units of D-galactose and 3,6-anhydro-galactose joined by α-1,3 and β-1,4-glycosidic linkage. The galactose polymer therein can be hydrolyzed into Galactooligosaccharide (GOS)type prebiotics. Hydrolysis was done by low concentration of sulfuric acid and crude enzyme from inactivation of Vibrio algynoliticus cell broth. Combination of prebiotic and probiotic provide lactic acid bacteria (BAL) as well as its food sources in the colon. The aim of this research is to know the characteristics of BALgrowth and fermentation in hydrolysateof E. cottonii and to know the best method in producing synbiotic powdersfrom BAL grown in hydrolysate. Analysis conducted include the characteristics of synbiotic powders using two types of BAL, namely Lactobacillus casei and kefir bacteria (Lactobacillus spp.). Characteristics of the two types of BAL produced during fermentation were qualified as probiotics which were amounted to more than 108 cell/mL, pH value <4.5, and total acid> 0.2%. The results showed that the number of living bacteria was higherin the method of synbiotic productions after dryingof hydrolysates followed by inoculum addition, than the method of synbiotic productions which inoculum addition was donebefore drying. Keywords:Eucheuma cottonii, Lactobacillus casei,Lactobacillus spp.in kefir
PENGEMBANGAN PRODUK OBAT KUMUR KONSENTRAT DENGAN BAHAN AKTIF MINYAK ATSIRI DAUN SIRIH DAN DAUN CENGKEH Dwi Setyaningsih; Siti Rahmi Nuabdi; Neli Muna
Jurnal Teknologi Industri Pertanian Vol. 29 No. 3 (2019): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24961/j.tek.ind.pert.2019.29.3.327

Abstract

Tooth and mouth disease that is suffered by many people is the reason for the increasing usage of mouthwash. Mouthwash on the market contains alcohol which causes side effects. The purpose of this study is to develop mouthwash concentrate based on essential oil (betel leaves and cloves) that can be accepted by prospective consumers, and to analyze the eligibility criteria of the mouthwash industry investment. The respondents 100 peoples, ranging in 15 to 62 y.o including visitors at Botani Square Mall, Sempur Field, Bhayangkara Hospital Bogor, and IPB students. The research uses an action research approach including market identification, prototype design, research and development, product acceptance analysis and financial analysis. Some problems raised from respondent interview were the side-effects of product, no measuring cup attached, less practical package, and the price was not affordable. The research solution was a practical 30 mL prototype of bottle drops with measuring cap. The production cost was IDR 6,808 and selling price (based on customer willingness to buy) of IDR 20,000. The result also showed a good acceptance of product by most respondents. Based on financial analysis, this product was concluded as feasible for industrial implementation. Keywords: alcohol mouthwash, betel-clove essensial oils, financial analysis, mouthwash industry
PROSES PEMURNIAN FRAKSI KAYA RHODINOL MINYAK SEREH WANGI MENGGUNAKAN SPINNING BAND DISTILLATION Arum Nur Fitrah; Meika Syahbana Rusli; Dwi Setyaningsih; Arief Riyanto; Nur Hidayati
Jurnal Teknologi Industri Pertanian Vol. 30 No. 2 (2020): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24961/j.tek.ind.pert.2020.30.2.128

Abstract

Indonesia, as one of the biggest countries which supplies citronella oil, has a high potential to produce natural rhodinol. Recent studies on rhodinol purification using fractional and mollecular distillation showed the difficulty of obtaining high purity and yield. The objective of this study was to determine the method of spinning band distillation to purify rhodinol regarding reflux ratio and vaporization temperature. Citronella oil was divided into three fractions according to its boiling points. The volume of each fraction was estimated by its ratio (according to GC analysis) to the feed volume: F1 (components before rhodinol) 21.73%, F2 (rhodinol) 61.7%, and residue 15.82%. Initial research established the best conditioning before the purification process was 3 mmHg of pressure, equilibration started at 160oC in 30 minutes, and 18–17% of heat rate, as these created the stability of the vapor temperature. The levels of reflux ratio were 3:1 and 5:1, while the evaporation temperatures of rhodinol were 230oC and 235 oC. Reflux ratio of 5:1 at 230oC obtained the highest purity (81.30%) and the highest yield (72.94%). Equilibration and reflux ratio allowed rectification inside the column because the spinning band homogenized the vapour molecules by its downwards flow and wiped the reflux on the inner surface, which in turn caused the molecules with a lower boiling point to evaporate gradually. Meanwhile the other yields were 79.81% (3:1 at 230 oC), 80.53% (3:1 at 235 oC), and 80.21% (5:1 at 23oC) with yields of 71.82%, 70.31%, and 68.23%. Keywords: citronella oil, purification, rhodinol, spinning band distillation
Sifat Fisiokimia dan Aroma Ekstrak Vanili Dwi Setyaningsih; Meika Syahbana Rusli; Nurmalia Muliati
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 3 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

 The curing process of vanilla beans from dried vanilla to vanilla extract would give added value to vanilla products. Aroma and taste in vanilla extract depend on variety of plants, cultivation methods, and curing process. Indonesian vanilla extract tend to give woody and phenolic aroma because it was harvested too early and it did not cure perfectly. This study was to identify the physicochemical and aroma characteristics of vanilla extracts from importer, exporter, and vanilla extracts from the newest experiment from our laboratory. There  were seven samples, three from importers (Tahiti grade I, Tahiti grade II, Virginia Dare), two from Indonesian Vanilla exporters (Djasula Wangi, Cobra), and two from our laboratory (G11, 57). The  physicochemical characteristics which were analyzed were vanillin content, ash, soluble ash, alkalinity of soluble ash, alkalinity of  total ash, total acidity, and lead number, all compared with the Food and Drugs Administration (FDA) standard. Sensory analysis used aroma description test consist of qualitative descriptive test (in-depth interview and focus group methods) and quantitative descriptive analysis. The result showed that the laboratory's sample from modified curing process (G11) followed the FDA standard in physicochemical characters, but the aroma description was not as strong as the aroma of vanilla extract from exporters, namely Cobra with creamy, sweet, and vanilla aroma; and vanilla extract from importer, namely Virginia with smoky and spicy aroma.   Keywords: vanilla extracts, aroma   
Co-Authors . Sugiyono Ade Kurniawan Agustiyana, Rosela Andini, Rina Widia Anggi Firdaus Agumsah Ani Suryani Ani Suryani Annisa Putri Anton Apriyantono Arief Riyanto Arum Nur Fitrah Atika Hermanda Bagus Ardi Saputro Bertoka Fajar SP Negara Chilwan Pandji Darmono Taniwiryono Dayu Dian Perwatasari Desi Musdaniaty Dewi Sondari Dina Prasetyowati Djumali Mangunwidjaja ediyansyah Edy Mulyono Egi Agustian Erliza Hambali Farah Fahma Dept. TIN Farida Anggraini Firdausi, Stabita Fitriana Roselly Fitriani Kasim Harahap , Nur Layla Syafrina Harto Nuroso Hasmani Hayuni, Okta Ros Hermanda, Atika Hery Haerudin Hery Haerudin Hery Haerudin I Komang Gede Wiryawan I Wayan Arnata Ika Mariska Intan Purnamasari Irma Rumondang Jaini Fakhrudin Jihan Suraya Juliejantiningsih , Yovitha Kartika Kartika Kartika Okta Purnama Khaidir Khaidir Khaidir Khaidir, Khaidir Khanifah, Rizka Nur Khaswar Syamsu Lawang, Andi Tenri Liesbetini Haditjaroko M Luqmanul Hakim Maggy T Soehartono Maulidayanti, Esti Mega Meika S Rusli Meika Syahbana Rusli Melawati . Mira Rivai Mira Rival Mira Sofyaningsih Mufidah, Ratna Muhammad Nuur Faiziin Muhammad Syukur Sarfat Mujizat Kawaroe Kawaroe Mulyono Mulyono Mulyorini Rahayuningsih Nastiti Siswi Indrasti Neli Muna Neli Muna Nelwan, Leopold Oscar Nevianti, Devi Rizka Ayu Ngasbun Egar Nur Hidayati Nur Richana Nurmalia Muliati Ovi Yulianti Nurmilah Perwira, Wanda Gema Pratama, Riski Danan Putri, Chika Juliana Rahmiyati Kasim Reni Rahmalia Rienoviar Rienoviar Rizal Syarief Robbani, Muhammad Haqqiyuddin Rumondang, Irma Sapta Raharja Silvester Tursiloadi Siska Desri Wirahmi, Busman, Edrizal, Siti Rahmi Nuabdi Sitti Wajizah Sri Windarwati Sriyanto Sriyanto Sudirman Sudirman Sudirman Sudirman Sugiyono . Syafira Salsabila Syahbana, Meika Tentri Yera Idqa Ridmaningrum Titi Candra Sunarti Tun Tedja Irawadi Tun Tedja Irawadi Vitta Rizky Permatasari Vusvitha, Vastha Wasmen Manalu Wiharja Wiharja Yuana Susmiati Yuliani, Manis Zulhamdan