WO2025024750A3 - Assessing nitrogenase proteins in rhizosphere to infer microbial functioning - Google Patents

Assessing nitrogenase proteins in rhizosphere to infer microbial functioning Download PDF

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Publication number
WO2025024750A3
WO2025024750A3 PCT/US2024/039701 US2024039701W WO2025024750A3 WO 2025024750 A3 WO2025024750 A3 WO 2025024750A3 US 2024039701 W US2024039701 W US 2024039701W WO 2025024750 A3 WO2025024750 A3 WO 2025024750A3
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rhizosphere
nitrogenase
infer
assessing
microbial
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French (fr)
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WO2025024750A2 (en
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Clayton NEVINS
Karsten TEMME
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Pivot Bio Inc
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Pivot Bio Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/26—Klebsiella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0095—Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/007—Determining fertilization requirements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/22—Klebsiella

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Agronomy & Crop Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Disclosed herein are methods, compositions, and kits for determining the presence or absence of a target polypeptide (e.g., a polypeptide involved in nitrogen fixation) in a sample (e.g., a sample of soil from the rhizosphere of a non-leguminous plant).
PCT/US2024/039701 2023-07-26 2024-07-26 Assessing nitrogenase proteins in rhizosphere to infer microbial functioning Pending WO2025024750A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363529025P 2023-07-26 2023-07-26
US63/529,025 2023-07-26

Publications (2)

Publication Number Publication Date
WO2025024750A2 WO2025024750A2 (en) 2025-01-30
WO2025024750A3 true WO2025024750A3 (en) 2025-03-06

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Families Citing this family (6)

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WO2020006064A2 (en) 2018-06-27 2020-01-02 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
EP3813511A4 (en) 2018-06-27 2022-08-03 Pivot Bio, Inc. GUIDED MICROBIAL REMODELING, PLATFORM FOR THE RATIONAL IMPROVEMENT OF MICROBIAL SPECIES FOR AGRICULTURE
AU2020206751A1 (en) 2019-01-07 2021-07-01 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
CA3129539A1 (en) 2019-04-25 2020-10-29 Min-Hyung RYU High-throughput methods for isolating and characterizing ammonium-excreting mutant libraries generated by chemical mutagenesis
CA3172321A1 (en) 2020-05-01 2021-11-04 Farzaneh REZAEI Stable liquid formulations for nitrogen-fixing microorganisms
CN120758421B (en) * 2025-07-28 2026-02-24 广东省农业科学院植物保护研究所 A strain of Bacillus polymyxa S437 and its application

Citations (4)

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WO2019084059A2 (en) * 2017-10-25 2019-05-02 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
WO2021221690A1 (en) * 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
US20220064658A1 (en) * 2020-08-28 2022-03-03 Shanghai Academy Of Agricultural Sciences Autotrophic nitrogen fixation genes and an autotrophic nitrogenase expression cassette for plants and uses thereof
WO2022146117A1 (en) * 2021-01-04 2022-07-07 베트올(주) Method for simultaneously diagnosing erwinia carotovora and phytophthora infestans in soil by using semi-quantitative lateral flow immunodiagnostic technique

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US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US9194859B2 (en) 2011-12-23 2015-11-24 Abbott Point Of Care Inc. Reader devices for optical and electrochemical test devices
KR102804055B1 (en) 2015-07-13 2025-05-08 피벗 바이오, 인크. Methods and compositions for improving plant traits
CA3001001A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
CN116904333A (en) 2017-01-12 2023-10-20 皮沃特生物公司 Methods and compositions for improving plant traits
CN111527057A (en) 2017-10-25 2020-08-11 皮沃特生物股份有限公司 Gene targets for nitrogen fixation targeting improved plant traits
US20220017911A1 (en) 2018-12-21 2022-01-20 Pivot Bio, Inc. Methods, compositions, and media for improving plant traits
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EP4149245A2 (en) 2020-05-13 2023-03-22 Pivot Bio, Inc. De-repression of nitrogen fixation in gram-positive microorganisms
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Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
WO2019084059A2 (en) * 2017-10-25 2019-05-02 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
WO2021221690A1 (en) * 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
US20220064658A1 (en) * 2020-08-28 2022-03-03 Shanghai Academy Of Agricultural Sciences Autotrophic nitrogen fixation genes and an autotrophic nitrogenase expression cassette for plants and uses thereof
WO2022146117A1 (en) * 2021-01-04 2022-07-07 베트올(주) Method for simultaneously diagnosing erwinia carotovora and phytophthora infestans in soil by using semi-quantitative lateral flow immunodiagnostic technique

Non-Patent Citations (3)

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QIN Y. ET AL.: "Cloning, prokaryotic expression and functional characterization of NifH gene from the associative nitrogen-fixing bacteria Klebsiella Variicola DX120E", IRAN. J. BIOTECHNOL., vol. 21, no. 3, E3451, 1 July 2023 (2023-07-01), pages 1 - 11, XP093234904 *
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