KR20020014190A - The manufacturing process of pure enviroment as bio-agricultural chemicals by use of exactracted Figue material, chitosan-alginic acid - Google Patents

Abstract

A mixture of 0.3-20% chitosan and chitosan oligosaccharides prepared by dissolving chitosan in a molecular weight range of 140 to 200,000 in a 1% acetic acid aqueous solution and an aqueous solution of alginic acid and alginic acid oligosaccharides having a concentration of 0.05-20% in separate water, especially fig tree, leaf It consists of 0.01-20% of the fruit extract, waste garlic stem (stem, leaf), waste onion leaf, tomato stem and leaf extract and juice, and tanza and waste citron extract, juice and pine needles. And other compositions, intended for use as a natural pollution-free pesticide or seed coating agent for the cultivation of bean sprouts or fruit seeds. In particular, bean, watermelon, and soybeans can be expected to promote germination, growth, and disease resistance, control of growing period, maintain freshness of herbs and soybeans, and prevent contamination and decay without using pesticides as an additive. Additives containing water-soluble (alpha, beta-type small molecule chitins, chitosan, chitosan origosaccharides and monosaccharides) and alginic acid or alginic acid oligosaccharides and monosaccharides for cultivating small horticulture, waste garlic stem extract, tomato stem extract and juice and tanza and waste citron A method for preparing a natural pesticide composition containing an aqueous solution containing 0.1-10% of extract, juice and pine needle solution and 0.1-1.0% by weight of stabilizer as a stock solution, and mixing the preparation composition with water and mixing 0.01 Fruit and vegetable production process and cultivation method to spray the crop with the composition of -1% concentration.

Description

The manufacturing process of pure enviroment as bio-agricultural chemicals by use of exactracted Figue material, chitosan-alginic acid}

The present invention is a method of immersion using fig tree, fruit, leaf, waste garlic stem (stem, leaf), waste onion leaf, tomato stem and leaf extract and juice and tanza and waste citron extract and juice, pine needle solution and the like The extract and the fractionated active material were obtained through an organic solvent, ie, methanol ethanol, butanol, by compression extraction, and the fig extract was separated, and the fig active material according to the antibacterial and antifungal test results was used. And it relates to the production of chitosan algingan and natural pollution-free pesticides for plant crops containing oligosaccharides as a composition of each substance. In the Dongbogam, figs are recorded as fruits with high sugar content and activity to the extent that they are widely used for medicinal use. Figs (Ficus carrical L) are widely used as processed foods because of their high activity, especially figs, Yeongam-gun, Jeonnam around 1990. It is cultivated as a local specialty, and unlike other fruits, figs are natural, completely pollution-free fruits that can be grown without using pesticides. Thus, figs have a strong active substance against their own pests, and may contain various bioactive components such as protein, ficin, vitamins and minerals. In addition, Jeonnam Yeongam and Muan-gun areas are nationwide onions and hemps, and after the fall season, all of the onion onion and garlic stands are incinerated or partially incinerated, causing enormous environmental pollution. In particular, the garlic and onions can be cultivated without pesticides, so they can act as an anti-pest. Some of the related applications show the wisdom of ancestors who use garlic in rice bags to fight and prevent pests. As such, microorganisms and pests that propagate in food or crops vary according to climate and environmental conditions, but most of them are developed due to the increase in population due to the decay caused by microorganisms. It is an important research subject. In recent years, excessive pesticide application has caused excessive pesticide residues from agricultural products, resulting in ups and downs of the media, and accordingly, limited pesticide residues in foodstuffs pose a health threat to pregnant women as well as newborns. Therefore, the development of pollution-free natural pesticides is indispensable and should be encouraged at the national level.

In particular, figs have been used as a hemorrhoids and athlete's foot treatment and insect repellent, its pharmacological effect is recognized and there are many active substances in fig fruit. Therefore, in this study, based on the results of the existing extraction and separation research developed by the present inventors, natural pesticides using a complex active substance were developed by directly using a substance which is a natural antimicrobial agent obtained from figs.

Chitosan, which is well known, has been used as a fertilizer by drying and grinding crabs, shrimps, etc. from previous experiences. This is because chitin and chitosan, which are present in the shell of crustaceans, are produced by the effects of soil and crop improvement. This is because it reduces the damage of crops. Thus, chitin or chitosan promotes the physiological function of plants, chitosan has antibacterial and antifungal action, artificial skin using biocompatibility, bioabsorbable sutures and cosmetic materials, wastewater treatment agent, food additives, health supplement food, It is used in plant growth promoters. Also hemostatic. According to a research report that promotes the growth of useful intestinal microorganisms, development using chitosan has been actively developed.

Alginic acid extracted from kelp, a brown algae, is a refined carbohydrate that is a polymer of D-mannuronic acid and L-guluronic acid. [Y. Yonemuto. J.Ferment. Bioeng., 75 (1993) 68-70, M. Natsume. Carbohydr. Res. 258 (1994) 187-197].

The present invention relates to a natural pesticide used for cultivation of horticulture and fruit crops, such as pollution-free sprouts using a fig extract active material containing fermented chitosan and alginic acid, and more particularly, figs containing chitosan and alginic acid in the cultivation of crops The present invention relates to a method for producing a natural pesticide used in crops such as bean sprouts and overgrowths, which grow faster and grow faster, which are not infected with rot, pests, and general germs by using an aqueous extract of an active substance.

Vegetables, such as bean sprouts and lettuce, have recently been used with excessive toxic pesticides, various plant growth hormones, synthetic antibiotics, and heavy metal fertilizers for the purpose of speeding up the growth of bean sprouts and preventing various diseases.

However, the use of various additives and spreading agents used in growing crops is advantageous from the grower's point of view, but the health of consumers who consume bean sprouts and vegetables directly is toxic such as residual pesticides. It acts as a threat. For example, indole (indol-3-yl acetic acid), which is excessively used in bean sprouts plants, is currently extremely limited in a certain allowable range because of its high toxicity and strong effect on the human body. At present, the trend is to completely ban the use, but it is possible to reduce the cost of cultivation by enabling the prevention of various diseases of the bean sprouts and the cultivation of properties, and in some cases, it is still used to avoid crackdown. Therefore, in the present invention, extracts using an organic solvent using a fig tree, fruit, leaves, etc., by using the extract and the above-mentioned chitosan and alginic acid and an additional waste resources extracts, promoting natural plant growth and natural pollution-free pesticide efficacy It relates to a method for producing natural medicine for crop cultivation.

Therefore, the present inventors apply the mixtures and fractions obtained by extracting the active ingredients from edible natural figs (leaves, trees and berries) as a result of earnestly researching to solve the aforementioned conventional crop cultivation problems. Fast growing and antimicrobial activity to grow bean sprouts and crops without using pests by using fig extract actives containing pollution-free natural chitosan and alginic acid solutions without using harmful plant growth accelerators, toxic pesticides and antibiotics. In particular, the present invention relates to the production of pollution-free natural pesticides for cultivation of fruit and vegetable crops, which are natural pesticides that increase the shelf life and stability of cultivation crops when used as a pest control effect and an insecticidal effector.

Figure 1 is a fig tree extraction and separation process chart, Figure 2 is a result of in vitro antimicrobial activity test of pollution-free natural biological pesticides using chitosan-alginic acid-garlic band containing fig separation component, Figure 3 shows the glucosamine sugar content Indicates

The present invention is a fig stock and fig raw milk juice using a juice or extract, such as seungwi woopin (Masui Dauphine), Bongsi or White Genoa species, Bongsi fig fruits, trees and leaves, and mixed preparation, waste garlic stem extract, tomato Stem extract and juice, and tanza and waste citron extract and juice, pine needles and juice containing 0.1 to 20%, respectively, are used.

Bean sprouts cultivation method using chitosan according to the present invention is a solution of alginic acid, alginic acid oligosaccharide having a concentration of 0.01-10% in a separate water of 0.05-7% chitosan, chitosan oligosaccharides and alginic acid oligosaccharides prepared by dissolving chitosan in 1% acetic acid aqueous solution Mix. And more specifically, 0.01-10% of fig extract and other compositions of waste garlic bark extract, tomato stem bar extract and juice, and tanza and waste citron extract and juice, pine needle solution 0.1-10% and stabilizer 0.1- A composition comprising a 1.0% by weight aqueous solution as a stock solution, which is diluted with water and sprayed at a concentration of 0.01 to 0.5%. Although chitosan used here can be used irrespective of molecular weight, the molecular weight use range is 200-100,000.

In the present invention, chitosan made by deacetylating chitin from crustaceans, such as crabs and shrimp, was used as a natural plant growth promoter. Many studies have reported that chitin and chitosan promote the growth function of plants. Specifically, chitosan is used for antibacterial activity and food additives. On the other hand, alginic acid extracted from kelp, brown algae, is a purified carbohydrate that has been reported to have antimicrobial activity and plant growth activity as a polymer of D-mannuronic acid and L-guluronic acid. Based on this evidence, alginic acid has a different antimicrobial activity depending on the molecular weight distribution, like chitosan. Therefore, alginic acid is used as a low molecular weight molecule of about 30,000-40,000 or alginic acid is processed into an alginate oligosaccharide to increase plant growth and increase antimicrobial activity. . As a typical example, studies using active substances have been reported. Examples of chitosan derivatives (reduced degree of carboxymethyl group 0.9 molecular weight 250,000) and the low molecular weight of chitosan and chito-oligosaccharides on the agar medium developed by Professor Helano et al. The cabbage slices were incubated for 25 days in the dark, and the weight of the callus formed therein was compared according to the respective addition conditions, and the growth of the galus added with carboxymethyl chitosan was inhibited, but the low molecular weight chitosan or chitosan-oligosaccharide 0.1% It was reported that the addition showed 1.2 times and 1.5 times callus growth promoting effects, respectively, compared with the others. Also carboxyl methylchitosan. Low molecular chitosan, chito- oligosaccharides. After dissolving high molecular weight chitosan in a solution of lactic water and soaking the radish seeds, sowing the seeds and planting them in the field, it was reported that the seeds were coated with low-molecular chitosan and chito-oligosaccharides and the yield was 12-20% higher. It is. However, coating the seed with high molecular weight chitosan showed no increase effect.

As such, chitosan derivatives have been reported to have a plant growth promoting effect depending on conditions, and since chitosan is obtained from a shrimp or crab shell, which is a natural material, it is not known to be toxic to the human body at all.

Therefore, in the present invention, by using chitosan, which is a natural product and a pollution-free resource, it was intended to improve the growth promotion of bean sprouts and vegetables and to prevent pests. Therefore, it is an object of the present invention to develop a formulation containing the active substance extracted from fig leaf, fruit, tree and the above-mentioned natural product supplement as a mixed formulation.

In the application method, make a mixed aqueous solution composed of 1% fig extract mixed with 0.1% chitosan and 0.1% alginic acid, soak seeds for 2 to 4 hours, soak seeds and sow seeds and sow with chitosan solution of the same concentration when sowing. After the sowing of the treated seeds, the seed lengths were compared, and the soaked seeds in which the chitosan solution was added did not add any seeds, but the chitosan, alginic acid, and an aqueous solution of figs had a lot of hair roots, and The germination growth of seeds treated with developed alginic acid-chitosan-fig had many roots, but showed a slightly thicker body. And there was no rotten disease in the stem and root of bean sprouts due to increased antimicrobial activity. In particular, 20 average bowls of 100 cm in diameter were examined and averaged. Alginate-chitosan-figure-treated seeds were treated with other untreated or chitosan solutions. There was no rotten disease at all, the fresher the stems, the thicker and stronger the stems and the faster the growth.

In addition, after growing bean sprouts, the bean sprouts grown with water were immersed in water for about 1/2, and after 3 days, the bean sprouts had decayed, but the bean sprouts grown with chitosan-alginic acid-fig solution were observed for more than 7 days under the same conditions. But there was no odor or discoloration of bean sprouts.

When the chitosan aqueous solution-alginic acid-figure extract is used as in the present invention, since the antimicrobial properties of chitosan-alginic acid-fig and the like do not cause the decay of the viscous substances released or secreted from the bean sprouts, the chitosan aqueous solution is used repeatedly several times. There is a number. Therefore, when growing bean sprouts, the amount of water can be reduced, and the waste water discarded is relatively reduced.

In addition, in order to find out how much glucosamine, which is a monomer of chitosan, is contained in bean sprouts grown with chitosan and alginic acid oligosaccharide aqueous solution, the residual amount of glucotm residues was quantitatively determined by MBTH method reported by Tsuji et al. First, the bean sprouts grown with water, chitosan solution, and oligosaccharide 0.05, 0.1, and 0.15% were removed, and only the stem part was tested. After freezing and pulverizing the stem of bean sprouts, a specimen was prepared by acetic acid decomposition, and 1 ml of an aqueous solution of hydrazone was added with 0.5% 3-methyl-2-benzodiazo, and left at room temperature for 1 hour. The absorbance was measured at 653 after 30 minutes by adding 1 ml of an aqueous solution of iron. The calibration curve was made by the same method with glucosamine hydrochloride. As a result of the above review, it is shown in Table 1 below.

TABLE 1

As can be seen in Table 1, in the cultivation of chitosan-alginic acid solution, the residual content of monosaccharides of gurucosamine and alginic acid in chitosan increased. In addition, the concentration of residual glucosamine-alginic acid monosaccharides grown at a high concentration of chitosan aqueous solution was high.

The present invention extracts and fractions the waste fig tree, the fig fruit, and the leaves by using an organic solvent. Tens of millions of tons of fig trees that are cut and discarded in springtime due to drunkards are causing enormous environmental pollution. Such a fig tree is crushed to a certain powder size with a sawdust mill and used as a pesticide and other feed for use as extracted or fermented with water, methanol, ethanol, butanol, and the like. Fig leaves are extracted and extracted with methanol or ethanol separately and used in the preparation of natural pesticides according to the mixing ratio in the aqueous solution mixed with chitosan and alginic acid. In the present invention, a method and efficacy of producing a natural pesticide containing a pesticide and pest control agent and an antimicrobial agent using a method of extracting or juice using a garlic garlic stand, onion leaves, lung citron, tanger in combination with the natural pesticide Invented. In particular, the preferred temperature during the production is concentrated under reduced pressure in the low temperature range of 20-70 degrees. It is also possible to modify the mixture composition by concentrating in the temperature range of 40-70 degrees.

Fig tree extract, fig leaf, fresh juice extract according to the present invention is used as an active ingredient of fig fruit is used in the manufacture of fig using natural antimicrobial.

The present invention is obtained by using a fig fruit, a natural substance, a mixed extract using methanol, ethanol, butanol in addition to organic solvents water, immersion method, anterior deposition method, and a pulverization method, and optionally at a temperature of 30-90 degrees, More preferred temperature range is concentrated under reduced pressure for 2 hours to 36 hours at 50-70 degrees and prepared as an additive raw material containing the weight parts according to each embodiment and the fig extract (wood, fruit, leaf) to obtain the emulsion According to the process, the raw juice of the fig is obtained, and the natural antimicrobial agent is manufactured and processed according to the processing procedure. And 0.1 to 7.0 parts by weight and 0.1 to 1.0 parts by weight of a stabilizer, respectively.

The present invention relates to an antimicrobial composition comprising from 0.1 to 7.0 parts by weight of natural fig extract and from 2 to 25% by weight of fig juice concentrate. In the composition of fig fruit, tree, leaf and the like, the extract and the fig juice concentrate as the active ingredient, when the fig active ingredient is used more than 25 parts by weight, the general antimicrobial agent of the fig activity becomes viscous to produce a viscous agglomeration method and shelf life Falls. In this case, the ancillary solution stability is lowered, such as having to mix with water again. For this reason, it is reasonable to develop a fig antimicrobial solution that can be used by mixing dispersant with a high concentration of fig concentrate.

The fig stabilizer according to the invention is from 0.1 to 1.0 and the aspartic acid, gulutamide acid or surfactant is from 0.02 to 0.04.

In the present invention, a stabilizer may be used for stabilizing the fig separation component. Stabilizers are used by selecting one or two of commonly used pollution-free aspartic acid, glutamic acid, sodium carbonate, and surfactants. It is preferable to use 0.1-1.0 weight part of stabilizers for the fig pesticide composition of this invention. When 0.1 part by weight or less of stabilizer is used, sufficient stabilization effect cannot be expected. Hereinafter, the present invention will be described in detail with reference to Examples, but the technical scope of the present invention is not limited to these Examples.

As already mentioned, the compounds prepared according to the present invention are alginic acid, chitosan, fig ingredient has its own antimicrobial and antifungal activity, and can be used as a fungicide as a prophylactic and therapeutic agent as well as to combat antimicrobial antifungal.

As a known and useful natural pesticide, it targets the common fungi Candida albigans, Sagaromyces cerevisiae and Aspergillus niger and Gram-negative bacteria G (-) Aureus coli ATCC10536, Servisia aureus. As a result of investigating the antimicrobial effect of the target compound according to the present invention, it can be confirmed that the prepared compound is a natural product in the candida fungus than the common fungal ketoconazole formulation against bacteria, but excellent antimicrobial activity. Therefore, the novel chitosan-alginic acid-figure compound compound is an antimicrobial agent having a well-known antifungal and antimicrobial effect and a pharmacological effect.

The complex compound according to the present invention may be administered in a pharmaceutically acceptable suitable formulation prepared using a pharmaceutically acceptable additive and a suitable carrier, and each infusion mixture compound such as chitosan-alginic acid-figure-garlic bar upon administration. It is a composition containing as an active ingredient, it is sprayed with a natural pesticide according to a certain dilution ratio. Depending on the application method, it is used as a fruit coating and seed germination disinfection and accelerator.

Minimum Inhibitory Concentration (MIC) for Natural Biopesticides in vitro ) Measurement

1. Use strain

The microorganisms used in this experiment were Escherichia coli ATCC 10536, Pseudo monas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 2 5923, Bacillus subtilis ATCC 6633, G. Candida utilis, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger 9029, Candida pseudotropicalis, Tourlopsis glabrata 89-5 were used.

2. Medium used

Bacterial media used in this experiment: Mueller Hinton broth, Mueller Hinton ager and fungal media: Sabouraud Dextrose Broth, Sabouraud Dextrose agar.

3. Preparation of each extract and assay medium and measurement of antimicrobial activity:

One). To make the fungal diluent, the preculture is diluted to a concentration of 104-106 CFU / ml.

2). Each sample is dissolved in a suitable solvent and then diluted in 2-fold dilution with medium.

3). Inoculate each test tube with bacterial solution.

4). Bacteria are incubated at 37 degrees for 24 hours and fungi are incubated for 36-48 hours.

5). After each incubation, shake the test tube to check the turbidity of the bacteria. After checking the turbidity, each was inoculated in Mueller Hinton ager, Sabouraud Dextrose agar medium.

6). After confirming the growth of the bacteria, determine the lowest test tube concentration at which growth is not recognized and determine the MIC by the broth serial dilution method as described above.

4. Antimicrobial activity of each compound isolated:

In vitro antimicrobial activity test (Minimum Inhibition Conenetration) of (Example 5 and control natural pesticides and ketoconazole as a comparative compound) is shown in the table below.

[See Fig. 2] (Unit: g / ml)

<Production Example 1>

Hereinafter, the present invention will be described in more detail based on the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention in any sense is not limited by these examples.

In the following Preparation Examples and Examples, a chitosan-alginic acid-figure compound was used, and in order to prepare chitosan, the degree of chitosan deacetylation was increased by a method reported by (CHKim, Korean Ind. Eng, 7, 118 1996) and the like. Experiment.

<Production Example 2>

The present invention relates to the production of natural pesticides using fig-alginic acid-chitosan-garlic stalks, fig fruit, leaf tree of Seungui-upup (Masui Dauphine), bongsi or white genoa, which are cultivated and produced in Samho-myeon, Yeongam-gun, Jeonnam The experiment was carried out. Fig tree was extracted or concentrated by distillation and compression using methanol, ethanol, and other organic solvents using a sawdust manufacturing machine, and concentrated under reduced pressure at 30-70 degrees. The fig fruit and the fig leaf were extracted and used by the method performed in the above examples. As a specific method, fig fruit is used as it is selected from methanol, ethanol, butanol, etc. as it is based on 10 kg. For example, methanol is added by 10-50 times the amount of solvent, extracted by immersion compression method or pulverization method at room temperature for at least 3 months. Then, the solvent is removed by using a vacuum concentrator at 25-70 ° C. Used.

Preparation Example 3 Preparation of Juice Extract

Figs were washed and dried, and ripened figs and immature fruits and fresh fruits were crushed and ground on the basis of 10 kg separately using 2-10 times selected organic solvents, and then juiced using a compress. And it was concentrated using a reduced pressure concentrator at a temperature of 25-70 degrees, stored in a refrigerator and tested.

Example 1

Put 10Kg of waste fig tree into the wood sawdust maker, grind it well, and add about 10 times the volume of methanol to the fig tree, and leave it at the temperature of 25-70 degrees for more than 30 days to extract enough figs. It was efficient when extracted up to about 100 days. Juice was carried out using a press. And the filter was concentrated using a vacuum condenser at a temperature of 25-70 degrees, stored in a refrigerator and tested.

Example 2

15 g of chitosan having a molecular weight of 300 to 200,000 was dissolved in 10% aqueous 1% acetic acid solution to give a final concentration of 0.2%. 100 g of soybeans are immersed in 1 L of 0.2% aqueous chitosan solution, oligosaccharide, alginic acid, alginic acid oligosaccharide, and 0.05-2% of fig extract for 2 hours.These beans are placed in a hydroponic cultivation bowl, and the solution of 0.2% chitosan solution is kept on bean sprouts. It was supplied in a spray state. Meanwhile, the growth rate was compared and examined while growing bean sprouts using water in the same manner.

The growth rate was compared with the change of bean sprout growth weight. As a result, after 8 hours of cultivation of bean sprouts, cultivation with chitosan solution than water sprouts completely eliminated infection with fungi, and black spots, brown strains, etc. were completely eliminated from bean sprouts, and the growth rate was about 81% faster. Showed.

Example 3

The pH of the chitosan aqueous solution was adjusted to 5.0-6.5 and pH 6.5-8.3, and then the pH was adjusted with acetic acid and caustic soda solution. As a result, the chitosan and alginic acid solution showed faster growth rate than the pH below 6.5 at the pH 6.5-7.3. At pH 5.0-6.0, the rate of sprout sprout growth was the same or slower than that of water sprouts, and the roots had brown and brown spots.

When planting, they were left with about 1/3 of the storage complex and filled with water. After 10 days, the odor came out and freshness was dropped from the bean sprouts. However, in the case of bean sprouts grown in aqueous chitosan solution, the same conditions were examined and no odor was generated even after 7 days, and the freshness of the bean sprouts was maintained. As a result of spraying water sprouts with chitosan-alginic acid solution (0.15 ~ 2% concentration), oligosaccharides and fig extract 0.05 ~ 0.2% three times, the bean sprout freshness was maintained for more than 90% for 100 hours. .

Example 4

Bean sprouts cultivation method using chitosan according to the present invention is mixed with 0.3-1% chitosan and chitosan oligosaccharides made by dissolving chitosan in 1% acetic acid aqueous solution and an alginate aqueous solution having a concentration of 0.05-2% in a separate water and Fig extract 0.01 -10% (manufacturing 5%) and other compositions, such as waste garlic bark extract, tomato stem bark extract and juice, and tanza and waste citron extract, juice and pine needle solution 0.1-10% (2% each manufacturing) and An aqueous solution containing 0.1% by weight of a stabilizer was prepared as a stock solution and stored and used.

Example 5

Bean sprouts cultivation method using chitosan according to the present invention is mixed with 0.3% chitosan and alginic acid oligosaccharides made by dissolving chitosan in 1% acetic acid aqueous solution and an alginate aqueous solution having a concentration of 5% in separate water, and 10% fig extract and other compositions This is a composition consisting of waste garlic stem extract, tomato stem stem extract and juice, and tanza and waste citron extract, juice and pine needles, each containing 0.5% of aqueous solution and 0.1-1.0% by weight of stabilizer as a stock solution. By spraying at a concentration of 0.01% to 0.3%.

Example 6

Bean sprouts cultivation method using chitosan according to the present invention is mixed with 0.3% alginic acid and chitosan oligosaccharides made by dissolving chitosan in 1% acetic acid aqueous solution and an alginate aqueous solution having a concentration of 10% in a separate water, and 5% fig extract and other compositions This is a composition consisting of waste garlic stem extract, tomato stem stem extract and juice, and tanza and waste citron extract, juice and pine needles, respectively, with an aqueous solution containing 0.7% and stabilizer 0.1-1.0% by weight, which are mixed with water. By spraying at a concentration of 0.01% to 0.3%.

Example 7

Bean sprouts cultivation method using chitosan according to the present invention is mixed with 0.5% chitosan and chitosan oligosaccharides made by dissolving chitosan in 1% acetic acid aqueous solution and an alginate aqueous solution having a concentration of 3% in separate water, and 4% of fig extract and other compositions Waste garlic stand extract, tomato stem stem extract and juice, and tanza and waste citron extract and juice, pine needle solution 3% and stabilizer 0.1-1.0% by weight of the aqueous solution composition as a stock solution and mixed with water By spraying at a concentration of 0.01% to 0.3%.

Example 8

Bean sprout cultivation method using chitosan according to the present invention is mixed with 5% chitosan made by dissolving chitosan in 1% acetic acid aqueous solution and an alginate aqueous solution having a concentration of 2% in separate water, and waste garlic with 3% fig extract and other compositions. A composition consisting of a large extract, tomato stem extract and juice, an aqueous solution containing 4% of tanza and waste citron extract and juice and pine needles and 0.1-1.0% by weight of stabilizer, respectively. Spray feeding at a 0.3% concentration.

Example 9

Bean sprouts cultivation method using chitosan according to the present invention is mixed with 5% chitosan oligosaccharides made by dissolving chitosan in 1% acetic acid aqueous solution and alginic acid solution having a concentration of 5% in separate water, and lungs with 2% fig extract and other compositions A composition containing as a stock solution of garlic bark extract, tomato stem bark extract and juice, and 2.7% of tanza and waste citron extracts and juices and pine needles, and 0.1-1.0% by weight of stabilizer, respectively. And spray feeding at a concentration of ˜0.3%.

Example 10

Fig. 10Kg of fig tree according to the present invention is mixed with an alginate aqueous solution having a concentration of 2% in a separate water using a fig wood sawdust manufactured using a sawdust machine and the waste garlic stand extract with 1% fig extract and other compositions, Tomado stem extract is a composition containing 2% each, it is fermented at 40-60 degrees for 12 hours or more to produce a solid (used as animal livestock feed). And a fermentation agent is mixed with water, filtered, and filtered to spray the natural pesticide at a concentration of 0.01 to 0.3%.

Example 11

The chitosan-alginic acid-figure extract solution contains 1-5% of each extract component by using chitosan-alginic acid-garlic and a sawdust manufactured by a machine using a fig tree, characterized in that pH 6.2-7.5. In particular, fig. Sawdust is forcedly fermented in a temperature range of 40-60 ° C., and methanol is added in three times the volume, followed by immersion extraction for at least 60 days, followed by mixing and diluting to prepare the vegetable natural pesticide.

As described in detail above, according to the present invention, when the bean sprouts are cultivated using chitosan aqueous solution, the cultivated bean sprouts have a glucosamine content of monomeric chitosan of 18% or more and freshness is maintained for a long time, as well as the chitosan aqueous solution used for growing bean sprouts. It can continue to be used for more than 80 days to reduce the occurrence of sewage, and to prevent the growth and rot of soybean sprouts cultivation method is productive. The use of waste resources can greatly reduce environmental pollution. Particularly, when the fig ingredient itself is an insecticide and an antibacterial substance, it uses the fig-chitosan-alginic acid-garlic stalk component which has antimicrobial activity. Having excellent efficacy can greatly contribute to improving the health of the people.

Claims (6)

A mixture of 0.3-10% chitosan and chitosan oligosaccharides prepared by dissolving chitosan in a molecular weight range of 140-100,000 in a 1% acetic acid aqueous solution and an aqueous solution of alginic acid and alginic acid oligosaccharide having a concentration of 0.05-10% in a separate water, and a fig tree, a leaf , Fruit extract and 0.01-20%, waste garlic stem, onion leaf stem extract, tomato stem stem extract and juice, and tanza and waste citron extract, juice and pine needle solution 0.1-10% and stable, respectively. A non-polluting natural agrochemical composition containing an aqueous solution containing 0.1-1.0 wt% of the agent as a stock solution, a method of producing seed coating agents and other compositions as a manufacturing method, and a crop of 0.01 to 1% of the composition by mixing the preparation composition with water Process and Cultivation of Fruits and Vegetables [Claim 2] The chitosan-alginic acid-figure extract aqueous solution is pH 6.2-7.5, and the chitosan-alginic acid-garlic bar and the fig tree are prepared using a solid material, which is a sawdust manufactured by a machine. Manufacturing method characterized in that it is a natural vegetable pesticide used by forced fermentation of the mixed formulation containing 1-5% in the temperature range 40-60 ° C According to claim 1, extracting fig fruit and leaves with an organic solvent, or extracting the extract by hydraulic pressure using an organic solvent, or concentrated under reduced pressure, or finely crushed wood using a sawdust manufacturing machine (1-5) Cm) Cut to size and extract it with organic solvent, fermented fig tree sawdust to 40-60 degrees, extract with organic solvent or extract directly with organic solvent, extract juice and use it as natural pesticide, and use as animal feed for livestock Fig. 1 extracts and extracts such as fig extract and waste garlic stem, lung onion stem, lung citron extract and juice, tomato stem and leaf extract and juice, pine needle liquid and juice solution 0.05 to 20% by weight and stabilizer 0.1 to 1.0 weight A chitosan mixed composition, comprising%. The method according to claim 3, wherein the solvent is methanol ethanol propanol butanol, chloroform, dichloromethane, normal nucleic acid, acetone, etc., and the fig tree is prepared in the form of sawdust, or the fig tree is chopped (length 1-5 Cm) or used. , Or sawdust fermented 25-80 degrees and extracted with water, methanol and ethanol The use of natural pesticides and pesticides using the composition as a feed for livestock, moisturizers in the manufacture of soap, cosmetic raw materials, fish farm anti-pathogenic and nutrients, sea-suned organic acid mixtures, etc. Food additives and feed antifungals