IT1391619B1 - Metodo per l'individuazione, selezione e analisi di cellule tumorali - Google Patents

Metodo per l'individuazione, selezione e analisi di cellule tumorali

Info

Publication number
IT1391619B1
IT1391619B1 ITTO2008A000814A ITTO20080814A IT1391619B1 IT 1391619 B1 IT1391619 B1 IT 1391619B1 IT TO2008A000814 A ITTO2008A000814 A IT TO2008A000814A IT TO20080814 A ITTO20080814 A IT TO20080814A IT 1391619 B1 IT1391619 B1 IT 1391619B1
Authority
IT
Italy
Prior art keywords
identification
selection
analysis
tumor cells
tumor
Prior art date
Application number
ITTO2008A000814A
Other languages
English (en)
Inventor
Nicolo' Manaresi
Gianni Medoro
Giuseppe Giorgini
Original Assignee
Silicon Biosystems Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ITTO2008A000814A priority Critical patent/IT1391619B1/it
Application filed by Silicon Biosystems Spa filed Critical Silicon Biosystems Spa
Priority to CN200980153704.XA priority patent/CN102272595B/zh
Priority to DK09775275.2T priority patent/DK2350647T3/en
Priority to ES17156147T priority patent/ES2910715T3/es
Priority to US13/127,640 priority patent/US10234447B2/en
Priority to EP17156147.5A priority patent/EP3185012B1/en
Priority to PL09775275T priority patent/PL2350647T3/pl
Priority to PCT/IB2009/007316 priority patent/WO2010052543A1/en
Priority to EP09775275.2A priority patent/EP2350647B1/en
Priority to ES09775275.2T priority patent/ES2624827T3/es
Priority to DK17156147.5T priority patent/DK3185012T3/da
Priority to JP2011535174A priority patent/JP5814124B2/ja
Priority to KR1020117013045A priority patent/KR101680619B1/ko
Priority to CA2742769A priority patent/CA2742769C/en
Priority to PT171561475T priority patent/PT3185012T/pt
Priority to PL17156147T priority patent/PL3185012T3/pl
Publication of ITTO20080814A1 publication Critical patent/ITTO20080814A1/it
Application granted granted Critical
Publication of IT1391619B1 publication Critical patent/IT1391619B1/it
Priority to US14/542,259 priority patent/US10895575B2/en
Priority to CY20171100513T priority patent/CY1118888T1/el
Priority to US16/297,341 priority patent/US12153040B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/70—Mechanisms involved in disease identification
    • G01N2800/7023—(Hyper)proliferation
    • G01N2800/7028—Cancer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Physiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Multimedia (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
ITTO2008A000814A 2008-11-04 2008-11-04 Metodo per l'individuazione, selezione e analisi di cellule tumorali IT1391619B1 (it)

Priority Applications (19)

Application Number Priority Date Filing Date Title
ITTO2008A000814A IT1391619B1 (it) 2008-11-04 2008-11-04 Metodo per l'individuazione, selezione e analisi di cellule tumorali
KR1020117013045A KR101680619B1 (ko) 2008-11-04 2009-11-04 종양세포의 확인, 선택 및 분석 방법
ES17156147T ES2910715T3 (es) 2008-11-04 2009-11-04 Método para la identificación, la selección y el análisis de células tumorales
US13/127,640 US10234447B2 (en) 2008-11-04 2009-11-04 Method for identification, selection and analysis of tumour cells
EP17156147.5A EP3185012B1 (en) 2008-11-04 2009-11-04 Method for identification, selection and analysis of tumour cells
PL09775275T PL2350647T3 (pl) 2008-11-04 2009-11-04 Sposób identyfikacji selekcji i analizy komórek nowotworowych
PCT/IB2009/007316 WO2010052543A1 (en) 2008-11-04 2009-11-04 Method for identification, selection and analysis of tumour cells
EP09775275.2A EP2350647B1 (en) 2008-11-04 2009-11-04 Method for identification, selection and analysis of tumour cells
CN200980153704.XA CN102272595B (zh) 2008-11-04 2009-11-04 鉴定、选择和分析肿瘤细胞的方法
DK17156147.5T DK3185012T3 (da) 2008-11-04 2009-11-04 Fremgangsmåde til identificering, udvælgelse og analyse af tumorceller
JP2011535174A JP5814124B2 (ja) 2008-11-04 2009-11-04 腫瘍細胞を同定、選択及び解析する方法
DK09775275.2T DK2350647T3 (en) 2008-11-04 2009-11-04 Method for identification, selection and analysis of tumor cells
CA2742769A CA2742769C (en) 2008-11-04 2009-11-04 Method for identification, selection and analysis of tumour cells
PT171561475T PT3185012T (pt) 2008-11-04 2009-11-04 Método para identificação, seleção e análise de células tumorais
PL17156147T PL3185012T3 (pl) 2008-11-04 2009-11-04 Sposób identyfikacji, selekcji i analizy komórek nowotworowych
ES09775275.2T ES2624827T3 (es) 2008-11-04 2009-11-04 Método para la identificación, la selección y el análisis de células tumorales
US14/542,259 US10895575B2 (en) 2008-11-04 2014-11-14 Method for identification, selection and analysis of tumour cells
CY20171100513T CY1118888T1 (el) 2008-11-04 2017-05-12 Μεθοδος αναγνωρισης, επιλογης και αναλυσης καρκινικων κυτταρων
US16/297,341 US12153040B2 (en) 2008-11-04 2019-03-08 Method for identification, selection and analysis of tumour cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITTO2008A000814A IT1391619B1 (it) 2008-11-04 2008-11-04 Metodo per l'individuazione, selezione e analisi di cellule tumorali

Publications (2)

Publication Number Publication Date
ITTO20080814A1 ITTO20080814A1 (it) 2010-05-05
IT1391619B1 true IT1391619B1 (it) 2012-01-11

Family

ID=41137040

Family Applications (1)

Application Number Title Priority Date Filing Date
ITTO2008A000814A IT1391619B1 (it) 2008-11-04 2008-11-04 Metodo per l'individuazione, selezione e analisi di cellule tumorali

Country Status (13)

Country Link
US (2) US10234447B2 (it)
EP (2) EP2350647B1 (it)
JP (1) JP5814124B2 (it)
KR (1) KR101680619B1 (it)
CN (1) CN102272595B (it)
CA (1) CA2742769C (it)
CY (1) CY1118888T1 (it)
DK (2) DK2350647T3 (it)
ES (2) ES2624827T3 (it)
IT (1) IT1391619B1 (it)
PL (2) PL3185012T3 (it)
PT (1) PT3185012T (it)
WO (1) WO2010052543A1 (it)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110093417A (zh) * 2018-01-31 2019-08-06 北京大学 一种检测肿瘤单细胞体细胞突变的方法

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CN102661941B (zh) * 2012-04-19 2015-04-22 湖南大学 基于拉曼活性纳米颗粒混合组装的循环肿瘤细胞的耦合增强sers高通量生物传感方法
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CN103122387B (zh) * 2013-02-05 2015-02-04 苏州华益美生物科技有限公司 循环肿瘤细胞(CTCs)荧光PCR快速超敏检测试剂盒及其应用
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CN103869060B (zh) * 2014-03-07 2017-02-01 复旦大学附属中山医院 基于磁珠和微流控芯片的循环肿瘤干细胞检测试剂盒
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