FI120045B - Behandling av cellulosamaterial och däri användbara enzym - Google Patents
Behandling av cellulosamaterial och däri användbara enzym Download PDFInfo
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- FI120045B FI120045B FI20051318A FI20051318A FI120045B FI 120045 B FI120045 B FI 120045B FI 20051318 A FI20051318 A FI 20051318A FI 20051318 A FI20051318 A FI 20051318A FI 120045 B FI120045 B FI 120045B
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- dsm
- acremonium
- enzymes
- thermophilum
- enzyme
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Claims (24)
1. Förfarande för att behandfa cellulosamaterial med celiobiohydro-las, endogiukanas och hetaglukosidas, kännetecknat av att ceflobiohyd- 5 roiaset innehälier en aminosyrasekvens med minst 80 % identitet med sekvens nr 28.
2. Förfarande enligt patentkrav 1, kännete c k n at av att endo-giukanaset innehälier en aminosyrasekvens med minst 80 % identitet med sekvens nr 10, 12, 14 elier 16, elier med ett enzymatiskt aktivt fragment därav. to
3. Förfarande enligt patentkrav 2, kännetecknat avattendo- glukanaset kan erhalias ur Thermoascus aurantiacus, Acremonium thermophi-lum, elier Chaetomium thermophilum.
4. Förfarande enligt: patentkrav 3, k ä n n e t e c k n at av att endo-glukanaset kan erhalias ur Thermoascus aurantiacus GBS 116239, Acre moni- 15 um thermophilum GBS 116240, elier Chaetomium thermophilum CBS 730.95.
5. Förfarande enligt patentkrav 1, kännetecknat av att beta-glukpsidaset innehälier en aminosyrasekvens med minst 80 % identitet med sekvens nr 22. 24 elier 26, elier med ett enzymatiskt aktivt fragment därav,
6. Förfarande enligt patentkrav 5, kännetecknat av att beta- 20 glukosidaset kan erhalias ur Thermoascus aurantiacus, Acremonium thermophilum, elier Chaetomium thermophilum, företrädesvis ur Thermoascus aurantiacus CBS 116239, Acremonium thermophilum CBS 116240, elier Chaetomium thermophilum CBS 730.95.
7. Förfarande enligt nägot av de föregäende patentkraven, kan- 25 netecknat av att eeliulosamaterialet ar lignocellulosamaterial.
8. Förfarande enligt nägot av de föregäende patentkraven, ka nne t e c k n a t av att man behandlar lignoceljufosamaterialet med minst ett yt-terligare enzym, företrädesvis xylanas, vilket företrädesvis innehälier en aminosyrasekvens med minst 80 % identet med sekvens nr 18 elier 20, elier med go ett enzymatiskt aktivt fragment därav.
9. Förfarande enligt patentkrav 8, kannet e c kn at av att xyfa-naset kan erhalias ur Thermoascus aurantiacus elier Acremonium thermophilum, företrädesvis ur Thermoascus aurantiacus CBS 116239, eiler Acremonium thermophilum CBS 116240.
10. Förfarande enligt nägot av de föregaende patentkraven, k a n ne tee k na t ay ai! enzymen tillförs i ceiluiosamateriaJet antingen samtidigt eller efter varandra.
11, Förfarande enligt nägot av de föregaende patentkraven, k ä n -5 n e t e c k n a t av att ätminstone ett av enzymen kodas av en gen som är lika- dan som den som inkorporerats i mikroorganismen med deponeringsnummer DSM 17326, DSM 17324, DSM17323, DSM 17729, DSM 16724, DSM 16726, DSM 16726, DSM 17325 eller DSM 17667.
12, Förfarande enligt patentkrav 1, k ä n n e t e c k n a t av att cellu-10 losamaferialet väljs ur gruppen bestaende av majsskörderester, prydnadshirs (switchgrass), balm av säd, sockerrörsbagass och träbaserade material.
13. Enzympreparat, som innehäller cellobiöhydrolas, endoglukanas och betagiukosidas, kännetecknat av att eellobiohydrolaset innehäller an aminosyrasekvens med nriinst 80 % identitet med sekvens nr 28.
14. Enzympreparat enligt patentkrav 13, kanne tee k n a t av att endogiukanaset innehäller en aminosyrasekvens med minst 80 % identitet med sekvens nr 10, 12, 14 eller 16, eller med ett enzymatiskt aktivt fragment därav.
15. Enzympreparat enligt patentkrav 14, k ä n n e t e c k n a t av att 20 endogiukanaset kan erhallas ur Thermoascus aurantiams, Acremonium ther- mophilum, eller Chaetomium thermophilum, företrädesvis ur Thermoascus au-rantiacus CBS 116239, Acremonium thermophilum CBS 116240, eller Chaetomium thermophilum CBS 730.95.
16. Enzympreparat enligt patentkrav 13, k a n n eteek n a t av att 25 betaglukösidaset innehäller en aminosyrasekvens med minst 80 % identitet med sekvens nr 22, 24 eller 26, eller med ett enzymatiskt aktivt fragment därav.
17. Enzympreparat enligt patentkrav 16, k ä n n e t e c k n a t av att betaglukösidaset kan erhallas ur Thermoascus aurantiacus, Acremonium 30 thermophilum, eller Chaetomium thermophilum, företrädesvis ur Thermoascus I aurantiacus CBS 116239, Acremonium thermophilum CBS 116240, eller Chaetomium thermophilum CBS 730,95.
18. Enzympreparat enligt nägot av patentkraven 13-17, k ä n n e -teckn at av att det innehäller ätminstone ett ytterligare enzym, företrädesvis 35 xylanas, vilket företrädesvis innehäller en aminosyrasekvens med minst 80 % identitet med sekvens or 18 eiler 20, eller med ett enzymatiskt aktivt fragment därav.
19. Enzympreparat enligt patentkrav 18, k ä n n e t e e k n a t av att xylanaset kan erhallas ur Thermoascus aurantiacus eller Acremonium ther- 5 mophiium, företrädesvis ur Thermoascus aurantiacus CBS 116239, eller Ac~ remonium thermophilum CBS 116240-
20. Enzympreparat enligt nagot av patentkraven 13 -19, kanne-t e c k n a t av att atminstone ei av enzymen kodas av en gen som är likadan sorrv den som inkorporerats i mikroörganismen med deponeringsnummer DSM 10 17326, DSM 17324, DSM 17323, DSM 17729, DSM 16724, DSM 16726, DSM 16725, DSM 17325 eller DSM 17667.
21. Enzympreparat enligt patentkrav 13, k ä n n e t e ck n a t av att det föreligger i form av använt odlingsmedium, pulver, granulat eller vätska.
22. Anvandnihg av ett enzympreparat enligt nagot av patentkraven 15 13 - 21 för att bryla ner ceiluiosamaterial.
23. Användning enligt patentkrav 22, känneteck n a t av aft cel-lulosamaterialet väljs ur gruppen bestäende av majsskörderester, prydnadshirs (swiichgrass), halm av säd, sockerrörsbagass och träbaserade material.
24. Användning av förfarandet enligt nagot av patentkraven 1 -12 i 20 en process för framställning av etanol ur ceiluiosamaterial.
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| ES06830936.8T ES2501944T3 (es) | 2005-12-22 | 2006-12-15 | Tratamiento de material celulósico y enzimas útiles en el mismo |
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| RU2008123954/10A RU2458128C2 (ru) | 2005-12-22 | 2006-12-15 | Способ обработки целлюлозного материала и используемые в нем ферменты |
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| EP12151850.0A EP2453013B1 (en) | 2005-12-22 | 2006-12-15 | Treatment of cellulosic material and enzymes useful therein |
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| BRPI0620287A BRPI0620287B1 (pt) | 2005-12-22 | 2006-12-15 | método para tratar material celulósico com celobioidrolase, endoglicanase e betaglicosidase, uso do mesmo, preparação de enzima, uso da mesma, vetor, célula hospedeira de fungo e cepa de escherichia coli, bem como método de preparação de um polipeptídeo e uso do mesmo |
| ARP060105762A AR058721A1 (es) | 2005-12-22 | 2006-12-21 | Triamiento de material celulosico y enzimas utiles en el mismo |
| TW095148542A TWI391489B (zh) | 2005-12-22 | 2006-12-22 | 處理纖維素材料之方法及用於彼之酶類 |
| ZA200804976A ZA200804976B (en) | 2005-12-22 | 2008-06-06 | Treatment of cellulosic material and enzymes useful there-in |
| US12/141,976 US20090042266A1 (en) | 2005-12-22 | 2008-06-19 | Treatment of cellulosic material and enzymes useful thererin |
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| US15/690,513 US20180044656A1 (en) | 2005-12-22 | 2017-08-30 | Treatment of Cellulosic Material and Enzymes Useful Therein |
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| FI20051318A FI120045B (sv) | 2005-12-22 | 2005-12-22 | Behandling av cellulosamaterial och däri användbara enzym |
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