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PERBAIKAN VIGOR BENIH KAYU KUKU (Pericopsis mooniana THW.) MENGGUNAKAN HORMONE PRIMING (GIBBERELLIC ACID) DAN UJI TETRAZOLIUM Shelia, Finandita Rizki; Isminingsih, Sulastri; Hermita, Nuniek; Rustam, Evayusvita
AGROTEKSOS, Jurnal Ilmiah Ilmu Pertanian Vol 34 No 3 (2024): Jurnal Agroteksos Desember 2024
Publisher : Fakultas Pertanian Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/agroteksos.v34i3.1237

Abstract

Benih kayu kuku yang mengalami kemunduran mutu benih masih dapat ditingkatkan vigor nya melalui pemberian hormone priming (gibberellic acid/GA3). Penelitian ini bertujuan untuk mengetahui konsentrasi hormone priming dan lama perendaman yang efektif untuk meningkatkan vigor dan konsentrasi uji cepat tetrazolium untuk menentukan viabilitas benih kayu kuku. Penelitian ini menggunakan rancangan acak lengkap (RAL) 2 faktor. Faktor pertama konsentrasi hormon GA3 yaitu 100 ppm, 300 ppm, dan 500 ppm. Faktor kedua lama perendaman yaitu 24 dan 48 jam. Hasil penelitian menunjukan perendaman benih konsentrasi GA3 300 ppm merupakan hormone priming yang efektif dalam meningkatkan daya hantar listrik, nilai perkecambahan, dan rata-rata waktu berkecambah. Pengujian tetrazolium menunjukkan bahwa benih kayu kuku viabel atau masih hidup yaitu benih berwarna merah karena terdapat endapan trifenil formazan.
Biocontrol efficacy of endophytic bacteria from Mimosa pudica L. against Fusarium oxysporum in kepok banana (Musa acuminata × balbisiana) in vitro Ismiah, Alvi; Saylendra, Andree; Isminingsih, Sulastri; Roidelindho, Kiki
Jurnal Ilmiah Pertanian Vol. 22 No. 1 (2025): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v22i1.25226

Abstract

Fusarium oxysporum f. sp. cubense (Foc) is a major fungal pathogen responsible for Fusarium wilt disease in banana plants, significantly reducing productivity and leading to substantial economic losses. This study aimed to evaluate the biocontrol potential of endophytic bacteria isolated from Mimosa pudica L. against Foc through an in vitro assay. A completely randomized design (CRD) with four treatments was employed, including a negative control (CP0), a positive control (CPF0), and two endophytic bacterial isolates from Mimosa pudica L. (BEP8 and BEP15), each with four replications. The antagonistic activity of these isolates was assessed using the dual culture method. The results demonstrated that BEP8 and BEP15 significantly inhibited Foc growth from 1 to 5 days after inoculation (DAI), with maximum inhibition rates observed on day 4, reaching 36.60% and 37.52%, respectively. These findings suggest that endophytic bacteria from Mimosa pudica possess potential as biocontrol agents against Foc. However, further molecular identification and greenhouse trials are necessary to confirm their efficacy in real-world applications.
Analisis Konsentrasi dan Jenis Plant Growth Promoting Rhizobacteria (PGPR) Terhadap Pertumbuhan Bibit Bawang Merah (Allium ascalonicum L.) Rachmawati Sabira, Adrea Oktavia; Isminingsih, Sulastri; Muztahidin, Nur Iman; Roidelindho, Kiki
Mediagro: Jurnal Ilmu-ilmu Pertanian Vol 21 No 1 (2025): Mediagro
Publisher : Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/mediagro.v21i1.12429

Abstract

The use of bulbs often reduces the quality of the results because the seeds often carry pathogens and reduce the productivity of shallots. One of the factors that causes the decline in the production of this plant is the lack of appropriate and effective cultivation technology. A potential solution is to improve seed quality by using organic fertilizers, especially PGPR fertilizers. This study aims to determine the concentration and type of PGPR that affect the growth of shallot seeds. The research method used was a randomized block design consisting of two factors, namely; PGPR concentration consisting of three levels, namely 10 ml / l (K1), 20 ml / l (K2), and 30 ml / l (K3). The second factor is the type of PGPR consisting of 3 levels, including: mimosa pudica roots (P1), bamboo roots (P2), and elephant grass roots (P3). The results showed that the PGPR concentration of 20 ml / l (K2) gave the best effect on the parameters of plant height and leaf length. Among the types of PGPR, bamboo roots (P2) gave the best results on the parameters of plant height, number of leaves and leaf length. There is also an interaction between the concentration and type of PGPR on plant height and leaf length parameters.