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All Journal Jurnal Biologi Tropis SEAS (Sustainable Environment Agricultural Science) Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan
Zaitun Ritaqwin Rita
Universitas Islam Kebangsaan Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology

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Identification of Arbuscular Mychorizae Fungi on Oil Palm in Bireuen, Aceh: Identification of Arbuscular Mychorizae Fungi on Oil Palm in Bireuen, Aceh Zaitun Ritaqwin Rita; Mizan Maulana; Nazalia
SEAS (Sustainable Environment Agricultural Science) Vol. 5 No. 2 (2021)
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.76 KB) | DOI: 10.22225/seas.5.2.3972.114-121

Abstract

Abstract Oil palm plantations in Aceh , especially in Bireuen, are generally planted on red-yellow podzolic soil types. Arbuscular mycorrhizal fungi (AMF) are fungi who are symbiotically associated with 97% of plant species. This study aims to identify the type and population of mycorrhizal spores from oil palm rhizosphere soil samples using culture trapping techniques (maize, sorghum, kudzu). This research was carried out in a greenhouse and Experimental Field, Faculty of Agriculture, Islamic National University of Indonesia, Bireuen, Aceh. Isolation, identification and observation of AMF colonization on plant roots was carried out at the Laboratory of Soil Biology, Faculty of Agriculture, Syiah Kuala University. The parameters included the number of arbuscular mycorrhizal fungi spores and the percentage of root colonization using the trapping culture method. The trapping culture method used 50 g of soil samples on oil palm rhizosphere which aged 5 months, 7 years, 9 years and 12 years. The results showed that the most dominant mycorrhizae found was the Glomus type. The mycorrhizae spores types found in the trapping culture were Glomus, Acaulospora and Gigaspora. The host plant that produced the most AMF spores was sorghum, while the highest AMF colonization of the three host plants was found in the roots of maize plants (78%) with very high criteria. Keywords: Oil palm, Culture trapping, AMF
Reducing ammonia levels in catfish cultivation water using several aquatic plants Mustaqim Mustaqim; Mutasar Mustasar; Yusrizal Akmal; Mida Wahyuni; T. Irfan Fajri; Zaitun Ritaqwin
Depik Vol 11, No 3 (2022): DECEMBER 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.83 KB) | DOI: 10.13170/depik.11.3.27723

Abstract

Catfish farming is one of the businesses that is very much in demand by the community, because it can improve the community's economy. However, not all catfish farming businesses can produce maximum results. This can be caused by several factors, both from feed, management and water quality. Decrease in water quality can be caused by feces and fish feed residue. Poor water quality can cause the growth process, physiology and level of fish behavior to be disturbed. Efforts that can be done is to use aquatic plants as phytoremediation. The plants used in this study were Azolla sp, Eichhornia crassipes, Lemna sp, Ipomoea aquatic. The design used in this study was a completely randomized design with 4 treatments and 3 replications. The parameters observed in this study were ammonia levels, pH, and plant population. The results showed that Eichhornia crassipes and Lemna sp were able to reduce ammonia levels in catfish cultured water by 0.01 mg/l at the end of the study.Keywords:AmmoniaWater plantsCatfish
The acdS Gene Expression of Rhizobacteria Isolated from Rhizospheric Soil of Pineapple under Stressful Condition Dori Kusuma Jaya; Sri Ismiani; Zaitun Ritaqwin
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5432

Abstract

The unfavorable rhizosphere environment for the growth of rhizobacteria and plants such as flooding and herbicide is relatively common for these rhizobacteria to express the acdS gene which encodes ACC deaminase. This study aims to observe the expression of the acdS gene in the rhizobacterial isolates from pineapple plants affected by the environmental stressors. The expression of the acdS gene was observed by growing the bacterial isolate on a Dworkin-Foster medium containing 3 mM.L-1 ACC and observing the RNA abundance of the isolate based on the Cq value in the RT-qPCR reaction. The selected bacterial isolates were then identified molecularly using the 16S rDNA gene. This study showed that 2 out of 7 bacterial isolates growing on a DF+ACC medium were selected for the RT-qPCR process. The two isolates came from flooding and herbicide stressors with Cq values ​​of 17.86 and 20.87, respectively. The two isolates were 1F and 2H which were identified molecularly as Burkholderia sp. and Pseudomonas psychotolerans in which both genera of bacteria commonly have the acdS gene. The two isolates can be used as inoculant candidates in the production of biological organic fertilizers as well as in their empowerment through land use management practices to help plants tolerate environmental stressors and promote plant growth.
Co-Authors Dori Kusuma Jaya Mida Wahyuni Mizan Maulana Mustaqim Mustaqim Mutasar Nazalia Sri Ismiani T. Irfan Fajri Yusrizal Akmal