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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Indonesia Chimica Acta Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Chemistry Indonesian Journal of Chemical Research Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Makara Journal of Science Analit: Analytical and Environmental Chemistry Indonesian Journal of Jamu

Wulan Tri Wahyuni

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia

Author-ID : 411386

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Antibacterial and Antibiofilm Activity of Daemonorops draco Resin Wahyuni, Wulan Tri; Purwanti, Sri; Batubara, Irmanida
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13554

Daemonorops draco has been reported for its antibacterial activity and empirically used for wound healing by Anak Dalam ethnic at Jambi Province, Sumatera-Indonesia. This study was performed to evaluate antibacterial and antibiofilm activity of D. draco resin collected from Jambi. D. draco resin was extracted using n-hexane, ethyl acetate and methanol, respectively. Antibacterial activity of the extracts was evaluated using agar diffusion method against Staphylococcus aureus and Eschericia coli, whereas the minimum inhibitory concentration (MIC) and minimum bacteriacidal concentration (MBC) was determined by microdilution method. In addition, antibiofilm activity was evaluated by violet crystal method. The result showed that extraction yield of ethyl acetate was higher than methanol and n-hexane. Ethyl acetate and methanol extracts of D. draco exhibited stronger antimicrobial activity against S. aureus compare to n-hexane extract. MIC and MBC of methanol extract and chromatographic fraction (F5.1) of ethyl acetate extract were 0.5 and 1.0 mg/mL, respectively. In addition, antibiofilm assay revealed that all extracts were inhibit initial attachment of bacteria cell in biofilm formation. This result revealed a novel information that Â D. draco extracts was potential as inhibitor of biofilm formation. TLC bioautography of D. draco extracts indicated that constituent with Rf of 0.71 performed antimicrobial activity against S. aureus. This finding expected to strengthen the scientific backup for utilization of D. draco by society.

Potensi Daerah Sidik Jari Spektrum Inframerah Sebagai Penanda Bioaktovitas Ekstrak Tanaman Obat Latifah Kosim Darusman; Rudi Heryanto; Mohamad Rafi; Wulan Tri Wahyuni
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 3 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.806 KB)

Efficacy and quality of medicinal plant extracts depend on chemical composition therein. Therefore, to ensure its efficacy orquality, the chemical composition of extracts have to be analyzed based on its chemical marker or its chemical pattern. This study tried to develop a method for extracts quality assay based on infrared spectrum in fingerprint region and extracts inhibition activity to xanthine oxidase. Five extracts from kunyit (Curcuma domestica), temulawak ( C xanthorriza), jahe (Zingiber officina/e), temukunci (Boesenbergia pandurata), and cabe jawa (Piper retrofractum) were tested to inhibit xanthine oxidase activity. The bioactive data of extracts and its infrared spectrum was analyzed using PCA and PLS-DA (partial least squarediscriminant analysis). Almost all extracts that were tested showed inhibition activity to xanthine oxidase activity, except the extract of jahe. The result showed that PCA can group all of extract into each of their region. Meanwhile, PLS-DA has shown to be a good prediction model for classifying extracts based on fingerprint region of its mR Spectrum and its biological activity. Keywords: fingerprint, mR spectra, compound marker, xanthine oxidase

Deteksi Simultan Kuersetin dan Rutin Menggunakan Screen-Printed Carbon Electrode Termodifikasi Grafena Tamida Elinda; Wulan Tri Wahyuni; Eti Rohaeti
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Syarif Hidayatullah State Islamic University

Kuersetin dan rutin merupakan senyawa antioksidan alami yang termasuk golongan flavonoid. Senyawa ini dapat dideteksi dengan beberapa teknik analisis, salah satunya dengan teknik elektrokimia. Pada penelitian ini, deteksi simultan kuersetin dan rutin dilakukan dengan teknik voltametri siklik menggunakan screen printed carbon electrode (SPCE) termodifikasi grafena. Elektrode disiapkan melalui modifikasi elektrode kerja SPCE dengan campuran grafena:natrium poliakrilat (1:1). Larutan etanol 70%:bufer fosfat pH 7 (6:4) digunakan sebagai elektrolit dalam pengukuran kuersetin dan rutin. Hasil penelitian menunjukkan puncak oksidasi kuersetin dan rutin terdeteksi pada potensial 0.179 V dan 0.310 V vs Ag/AgCl. SPCE termodifikasi grafena menghasilkan intensitas arus oksidasi dan sensitivitas yang lebih tinggi pada pengukuran kuersetin dan rutin. Deteksi simultan kuersetin dan rutin menggunakan SPCE termodifikasi grafena menunjukkan linearitas dengan R2 = 0.9905 dan R2 = 0.9816, masing-masing untuk kuersetin dan rutin. Limit deteksi pengukuran kuersetin dan rutin masing-masing sebesar 0.5369 mM dan 0.7495 mM serta limit kuantitasi sebesar 1.7898 mM dan 2.4985 mM. Presisi pengukuran kuersetin dan rutin memiliki nilai simpangan baku relatif (%SBR) sebesar 2.14% dan 2.44%, berturut-turut untuk kuersetin dan rutin. Hal ini menunjukkan bahwa SPCE termodifikasi grafena cukup potensial untuk deteksi simultan kuersetin dan rutin secara elektrokimia. Kata kunci: Grafena, kuersetin, rutin, screen printed carbon electrode, voltametri siklik. Quercetin and rutin are natural antioxidant from flavonoid group. The compounds can be detected using several analytical technique, including electrochemical technique due to its redox activity. In this study, simultaneous detection of quercetin and rutin was performed at graphene-modified screen printed carbon electrode (SPCE) using cyclic voltammetry technique. Graphene-modified SPCE was prepared from mixture of graphene:sodium polyacrylate (1:1) via drop casting method. Ethanol 70%:phosphate bufer pH 7 (6:4) was used as electrolyte solution for quercetin and rutin measurement. The result shows that oxidation peak of quercetin and rutin was detected at potential of 0.179 V and 0.310 V vs Ag/AgCl, respectively. Graphene-modified SPCE produce higher intensity of oxidation current and higher sensitivity for quercetin and rutin measurement. Simultaneous detection of quercetin and rutin at graphene modified SPCE provide linearity with R2 = 0.9905 and R2 = 0.9816, respectively for quercetin and rutin. Detection limits of quercetin and rutin respectively was 0.5369 mM and 0.7495 mM while limit quantitation was 1.7898 mM dan 2.4985 mM. Precision of quercetin and rutin measurement was moderate with percent relative standard deviation (%RSD) of 2.14% and 2.44%, consecutively for quercetin and rutin. This suggests that graphene-modified SPCE is potential for simultaneous electrochemical detection of quercetin and rutin. Keywords: Cyclic voltammetry, graphene, quercetin, rutin, screen printed carbon electrode.

AKTIVITAS PENGHAMBATAN α-GLUCOSIDASE OLEH KOMBINASI EKSTRAK ETANOLIK BUAH Phaleria macrocarpa (SCHEFF.) BOERL. DAN DAUN Annona muricata LINN. Era Rahmi; Wulan Tri Wahyuni; Latifah Kosim Darusman; Irma Herawati Suparto
Majalah Obat Tradisional Vol 21, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Antidiabetic activities of Phaleria macrocarpa fruits and Annona muricata leaves through α-glucosidase inhibition have been reported. Each extract of the two medicinal plants showed weak α-glucosidase inhibitory activities. The effect of synergies is expected from combining the two extracts. The purpose of this research was to investigate α-glucosidase inhibitory activity of the combination of ethanolic extract of P. macrocarpa fruits and A. muricata leaves and to confirm the presence of different chemical constituents of the most active individual and extracts combination by thin layer chromatography (TLC). Extracts were obtained by maceration method using 96% ethanol. Various concentration of extracts combination was analyzed based on IC50 of the enzyme inhibition of each individual extract. The result showed that α-glucosidase inhibitory activity of each extracts was concentration dependent, with IC50 values of individual extracts of P. macrocarpa fruits and A. muricata leaves were 261.34 and 428.79 μg/mL, respectively. However, the extracts combination with the highest inhibition activity (84.51±0.79%) was obtained at its IC50 of P. macrocarpa fruits and two-third IC50 concentration of A. muricata leaves. The TLC profile of the most active extract combination showed a phytochemical constituent that is also detected in the profile of each individual extract. This can be assumed as a synergistic effect due to increased phytochemical contents of the combined extract.

Voltammetric Detection of Uric Acid at Screen Printed Electrode: A Review Wulan Tri Wahyuni; Dyah Iswantini Pradono; Selvitia Ulandari
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 14, No 2: December 2021
Publisher : Hasanuddin University

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Uric acid is a compound produced from purine metabolism. The excess amount of these compounds could causes diseases in the body. Effective and practical methods are needed for early detection of uric acid. Technically, uric acid could be detected using enzymes (enzymatic) and without enzymes (non-enzymatic). Several methods have already reported for uric acid detection in biological samples, including fluorimetry, colorimetry, liquid chromatography, flow injection analysis, chemiluninescence, and electrochemical detection. Among electrochemical detection methods, voltammetry is one of the most prospective for uric acid detection. This review covers the approach of uric acid detection including enzymatic and nonenzymatic approach, several voltametric technique for uric acid detection, and screen printed and modified electrode as platform for uric acid detection. Analytical performance such as linear range, sensitivity, selectivity and reproducibility of uric acid detection using various voltametric method and at different platform were also highlighted.

Efektivitas Krim Anti Jerawat Kayu Secang (Caesalpinia sappan) Terhadap Propionibacterium acnes pada Kulit Kelinci SITI SA’DIAH; LATIFAH KOSIM DARUSMAN; WULAN TRIWAHYUNI; IRMANIDA BATUBARA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (719.927 KB)

Sappan Wood extract (Caesalpinia sappan) is potential as an anti-acne due to its ability as an antioxidant, lipase inhibitor and anti Propionibacterium acnes. The potency is influenced by the quality of simplicia and extracts. To elevate the application, semisolid dosage formulations were made in cream forms using a standardized extract of Sappan Wood. Creams were formulated with the same base composition but different extract concentrations (range of 1-10%). In vivo assay were carried out on rabbit skin induced by intradermal injection of P. acnes. Sappan Wood was collected from Semarang and the resulting extract has met the Materia Medika Indonesia requirement with brazilin level in extract of 224.675 mg / g. In vivo tests showed that all Sappan Wood extract formulations have potency as anti-acne as indicated by curative percentage for 15 days were significantly different from the formula base. Curative effect of all formula were not significantly different with the Mediklin® positive control. Formula with the lowest concentration of the extract was the most effective and efficient with the curative percentage of more than 96% in 15 days.

Metode Ekstraksi dan Pemisahan Optimum Untuk Isolasi Xantorizol dari Temulawak (Curcuma xanthorrhiza) Wulan Tri Wahyuni; Herdiyanto Herdiyanto; Mohamad Rafi
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Xantorizol merupakan senyawa penciri utama temulawak (Curcuma xanthorrhiza). Penelitian ini bertujuan menentukan metode ekstraksi dan pemisahan optimum untuk isolasi xantorizol dari rimpang temulawak. Maserasi dan sokletasi digunakan untuk mengekstraksi xantorizol dengan pelarut metanol, dietil eter, dan n-heksana. Pemisahan dilakukan dengan kromatografi kolom dan kromatografi lapis tipis (KLT) preparatif dan hasil pemisahan dikarakterisasi berdasarkan spektrum inframerah dan kromatografi gas-spektrum massa (KG-SM), sementara persentase kemurniannya ditentukan dengan kromatografi cair kinerja tinggi. Ekstrak n-heksana dari teknik maserasi memiliki kandungan xantorizol lebih tinggi dibanding ekstrak lainnya yaitu sebesar 168 mg/g sampel. Fraksi ke-4 hasil pemisahan kolom terhadap ekstrak n-heksana memberikan dua spot pada KLT dengan Rf 0.54 dan 0.68, spot dengan Rf 0.54 diduga merupakan xantorizol (dikonfirmasi dengan KG-SM). Pemurnian lebih lanjut dengan KLT preparatif terhadap fraksi ke-4 menghasilkan xantorizol dengan rendemen sebesar 0.016 % berdasar bobot sampel dan tingkat kemurnian sebesar 87.40 %.

Analisis Sidik Jari Kromatografi Lapis Tipis Rimpang Temu Mangga (Curcuma mangga) Makmum Syafi’i; Eti Rohaeti; Wulan Tri Wahyuni; Mohamad Rafi; Dewi Anggraini Septaningsih
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Profil sidik jari kromatografi lapis tipis (KLT) dapat dimanfaatkan untuk kendali mutu bahan baku maupun produk tumbuhan obat. Penelitian ini bertujuan mengembangkan metode analisis sidik jari rimpang temu mangga (Curcuma mangga) untuk kendali mutunya. Rimpang kering temu mangga diekstraksi menggunakan metanol dan dianalisis sidik jari KLT. Kloroform:etil asetat (8.5:1.5) merupakan fase gerak optimum yang memisahkan 11 pita dari rimpang temu mangga dengan keterpisahan yang baik dan memiliki pita khas biru tua (Rf 0.36). Demetoksikurkumin terdeteksi pada rimpang temu mangga dengan warna jingga kecoklatan pada UV 366 nm (Rf 0.48) setelah diderivatisasi dengan pereaksi asam sulfat 10%. Pengujian spesifitas terhadap rimpang lain dalam satu genus, rimpang temu mangga menunjukan pola yang berbeda. Validasi metode analisis sidik jari KLT memenuhi kriteria keberterimaan, sehingga metode ini dapat digunakan untuk kendali mutu rimpang temu mangga.

Optimization and Validation of High Performance Liquid Chromatographic Fingerprint of Phyllanthus niruri Wulan Tri Wahyuni; Latifah K. Darusman; Aji Hamim Wigena
Indonesian Journal of Chemistry Vol 14, No 2 (2014)
Publisher : Universitas Gadjah Mada

Split-plot mixture-mixture design has been applied for optimization of Phyllanthus niruri chromatographic fingerprint. The design was applied for unreplicated and simultaneous optimization of extraction mixture and chromatographic mobile phase mixture. The whole-plot was extraction solvent contained various combinations of methanol, ethyl acetate and dichloromethane in a simplex centroid with axial design. The sub-plot was reversed phase chromatographic mobile phase in simplex centroid design contained varying proportions of methanol, acetonitrile and acetonitrile:water (55:45 v/v). Each extract was eluted with seven mobile phases of high performance liquid chromatography and detected at 210, 225 and 254 nm. The optimum condition was determined based on the number of peaks. The correlation between extraction solvent, chromatographic mobile phase and number of peak were evaluated with Ordinary Least Square method. The root mean square error of calibration and root mean square error of prediction obtained at 210, 225 and 254 nm were 1.8634 and 4.0076; 2.2220 and 5.2839; 1.5436 and 2.2606, respectively. The optimum condition was obtained when the ethyl acetate extract was eluted with acetonitrile:water (55:45 v/v) and detected at 254 nm. Precision of retention time at optimum condition was excellent, with the relative standard deviation in a range of 0.0698% and 0.3006%.

FIRST-ORDER ULTRAVIOLET DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF RESERPINE IN ANTIHYPERTENSION TABLET Latifah K Darusman; Mohamad Rafi; Wulan Tri Wahyuni; Rizna Azrianiningsari
Indonesian Journal of Chemistry Vol 12, No 3 (2012)
Publisher : Universitas Gadjah Mada

A new ultraviolet derivative spectrophotometry (UVDS) method has been developed for determination of reserpine in antihypertension tablets. A first-order UVDS based on the measurement of the distance between peaks to baseline (DZ) at the wavelength of 312 nm was used. Evaluation of analytical performance showed that accuracy as percentage recovery was 99.18-101.13%, precision expressed as relative standard deviation (RSD) was 1.91% and linear correlation was also obtained 0.9998 in the range of 10-50 µg/mL. Estimation of limit of detection and limit of quantitation was 0.8868 µg/mL and 2.6874 µg/mL, respectively. As a reference method, HPLC methods from United States Pharmacopiea (USP) were used. Commercially tablets available were analyzed by the two methods. The content of reserpine in tablets was found 0.2260±0.0033 mg by UVDS and 0.2301±0.0051 mg by the USP methods. The result obtained from the two methods was compared statistically using F-test and t-test and showed no significant differences between the variance and mean values of the two methods at 95% confidence level. This method was faster, easier, low cost and gave result as well as the reference method published by USP.

Co-Authors Abdul Rohman Achmad Fauzi Achmad Fauzi Aditya Widianto Agus Kartono Ahmad, Shahrul Nizam Aji Hamim Wigena Aninda Naszwa Kuswanto Anindya, Weni Antonio Kautsar Apriliani Rahmat Asoka, Shadila Fira Badrunanto Badrunanto Badrunanto, Badrunanto Bambang Prajogo Eko Wardoyo Budi Riza Putra Budi Riza Putra Budi Riza Putra Desi Herliani Dyah Iswantini Era Rahmi Erna Subroto Eti Rohaeti Fauziyatul Munawaroh Fransiska Devy Junardy Hera Nirwati Herdiyanto Herdiyanto Ika Kartika Irma Herawati Suparto Irmanida Batubara Karomah, Alfi Hudatul Kilala Tilaar Latifah K Darusman Latifah K Darusman Latifah K Darusman Pitria Latifah K. Darusman Latifah Kosim Darusman Latifah Kosim Darusman LATIFAH KOSIM DARUSMAN Luthfiana, Widya M. Rafi Mahat, Mohd Muzamir Maily Mustofa Makmum Syafi’i Manggar Arum Aristri Moh Arif Rohman Nur Hakim Mohamad Rafi Mohamad Rafi Mohamad Rafi Monica Ina Martha Muhammad Bachri Amran Muhammad Farid Munawaroh, Fauziyatul Nevianti Zamany Nisa, Ulfiatun Novik Nurhidayat Nur Zamilah Nurdiani Nurdiani Nursifa Mawadah Ramadhanti, Desi Rany Zalvianita Reni Febriani Ria Apriana Ridhova, Aga Rika Indri Astuti Rizna Azrianiningsari Rudi Heryanto Saat Egra Salmi Afifah Saskito, Dinda Iryawati Bedy Selvitia Ulandari Septaningsih, Dewi Anggraini Setyanto Tri Wahyudi Silvia, Riva Silviani, Dea Siti Aisyah SITI SA’DIAH Sri Purwanti Sri Purwanti Sri Purwanti Sukendi, Yeli Suparto, Irma Syarifah Meutiah Eka Sari Syifa Muflihah Tamida Elinda Thaha, Yudi Nugraha Ulfiatun Nisa Utami Dyah Syafitri Waras Nurcholis Widya Luthfiana Wulansari, Laela Yessie Widya Sari Yogo Suro Priyadi

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