Claim Missing Document
Check
Articles

The Economies of Scope in Agricultural Productions: A Case of Jogjakarta Province Mariyono, Joko; Pawukir, Enny Suswati
TEMA Vol 4, No 2 (2003)
Publisher : Fakultas Ekonomi dan Bisnis Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18202/tema.v4i2.168

Abstract

The objective of this study is to identify the performance of regional productions of rice and soybean that have been jointly cultivated during a year at the same irrigated lands. The study is motivated by the fact that decentralisation of governments has been implemented, and consequently the local resources possessed by local region need to be exploited economically. This study uses the concept of economies of scope as underpinning theory. Pooled estimation is used for estimating product transformation curve that describes the relationship between soybean and rice productions. Data consisting of four districts and eleven years used in this analysis is collected from publication of local statistical offices. The results of study indicate that yearly joint production of rice and soybean shows degree of economies of scope, implying that the level of joint products is physically higher than that of separated product. However, under given market prices, the profitability of joint productions is economically low, compared with single production of rice. This is because yield of soybean is low, and the relative price of soybean is not too high. It is therefore, in this case, more money-making to grow rice as much as possible in the irrigated lands. Keywords: economies of scope, product transformation curve, rice and soybean
The Effect of Mirabilis jalapa Leaf Ethanolic Extract against Streptococcus pyogenes Pambudi, Bagus Satrio; Suswati, Enny; Firdaus, Jauhar
Journal of Agromedicine and Medical Sciences Vol 2, No 1 (2016)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Streptococcus pyogenes is a Gram positive bacteria that commonly cause disease in human. If not treated immediately, this bacteria can cause serious complication such as reumatic fever that causing heart valve tissue damage. Penicilin, drug of choice to eradicate S. pyogenes, oftenly cause various side effects such as anaphylaxis. This study aimed to investigate the effect of Mirabilis jalapa leaf ethanolic extract against S. pyogenes growth using in vitro techniques. The study design was a quasi experimental design. S. pyogenes culture as the study subject were divided into positive control group (penicilin V 100 IU), negative control group (NaCMC 0,5%), and eight treatment groups that were given with M. jalapa leaf ethanolic extract as much as 0,1 mg/ml, 1 mg/ml, 5 mg/ml, 10 mg/ml, 20 mg/ml, 30 mg/ml, 40mg/ml, and 50 mg/ml. After 24 hours incubation periods, the inhibition zone were found in all treatment group except in concentration 0,1 mg/ml. This study showed that M. jalapa leaf ethanolic extract could inhibit the growth of S. pyogenes. There was significant correlation between the concentration of M. jalapa leaf ethanolic extract and the diameter of inhibition zone (p=0,00), the higher concentration of M. jalapa leaf extract, the larger diameter of inhibition zone of S. pyogenes. Keywords: Mirabilis jalapa, leaf extract, antimicrobial activity
Protein Haemaglutinin Outer Membran Protein (OMP) 35 kDa sebagai Protein Adhesin Proteus mirabilis pada Vesika Urinaria Kelinci Suswati, Enny; Mufida, Diana Chusna
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.982 KB) | DOI: 10.31258/jnat.12.2.136-142

Abstract

Proteus mirabilis is opportunistic and nosocomial pathogen that usually found in clinical specimen from patientswith catheter. The pathogenic mechanism of the bacteria are not fully elucidated especially its potential activity ofthe protein as hemaglutinin and adhesion molecule. The aim of this study is to evaluate the role of 35 kDa outermembrane protein from P. mirabilis. After identification, bacterial isolate of OMP fraction 12,5% SDS-PAGE wereused to isolate OMP followed by hemaglutinin test and invitro adhesion test. The study showed that the 35 kDa OMPof P. mirabilis was a hemaglutinin protein that could agglutinate mice erythrocytes, rabbit erythrocytes, and humangroup O erythrocytes. Hemaglutination test were negative on erythrocytes human blood group A,B, and AB. The 35kDa OMP was also adhesion protein showed by its activity to adhere to the rabbit vesica urinaria epithel receptor.The increase dose of 35 kDa OMP will decrease the amount of P. mirabilis bacteria to adhere to rabbit vesicaurinaria epithel (p< 0,05 ).
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
DIFFERENCES IN THE EFFECTIVENESS OF SELECTIVE ALPHA BLOCKER THERAPY COMBINED WITH 5-ALPHA REDUCTASE INHIBITOR ON IPSS VALUE OF BPH LUTS PATIENTS Wahyudi, Septa Surya; Probosutiksna, Nuno Febrian; Suswati, Enny
Indonesian Journal of Urology Vol 27 No 2 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i2.615

Abstract

Objective: The purpose of this study is to know the differences in effectiveness between SAB administration with combination 5-ARI administration assessed by IPSS evaluation in BPH LUTS patients. Material & Methods: This observational analytic with a cross-sectional design study had been done to 50 BPH LUTS patients that obtained at Paru Jember, Bina Sehat Jember, and Bhayangkara Bondowoso Hospital. These samples had got by simple random sampling method and include in inclusion with exclusion criteria freely. Results: This comparison study between the type of medical therapy administration and total IPSS decrease, IPSS-voiding decrease, IPSS-storage decrease, IPSS-QoL, and prostate volume give significancies as follows: p=0.002; p<0.001; p<0.001; p<0.05; p<0.001. Conclusion: There are significant differences between the type of medical therapy (SAB or 5-ARI combination) and total IPSS decrease, IPSS-voiding decrease, IPSS-storage decrease, IPSS-QoL, and prostate volume.
DIFFERENCES IN THE EFFECTIVENESS OF SELECTIVE ALPHA BLOCKER THERAPY COMBINED WITH 5-ALPHA REDUCTASE INHIBITOR ON IPSS VALUE OF BPH LUTS PATIENTS Wahyudi, Septa Surya; Probosutiksna, Nuno Febrian; Suswati, Enny
Indonesian Journal of Urology Vol 27 No 2 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i2.615

Abstract

Objective: The purpose of this study is to know the differences in effectiveness between SAB administration with combination 5-ARI administration assessed by IPSS evaluation in BPH LUTS patients. Material & Methods: This observational analytic with a cross-sectional design study had been done to 50 BPH LUTS patients that obtained at Paru Jember, Bina Sehat Jember, and Bhayangkara Bondowoso Hospital. These samples had got by simple random sampling method and include in inclusion with exclusion criteria freely. Results: This comparison study between the type of medical therapy administration and total IPSS decrease, IPSS-voiding decrease, IPSS-storage decrease, IPSS-QoL, and prostate volume give significancies as follows: p=0.002; p<0.001; p<0.001; p<0.05; p<0.001. Conclusion: There are significant differences between the type of medical therapy (SAB or 5-ARI combination) and total IPSS decrease, IPSS-voiding decrease, IPSS-storage decrease, IPSS-QoL, and prostate volume.
Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Shofia, Lailatis; Hermansyah, Bagus; Suswati, Enny; Agustina, Dini; Mufida, Diana Chusna; Shodikin, Muhammad Ali
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.153

Abstract

Tuberculosis (TB) and intestinal worms are infectious diseases with a large number of sufferers in Indonesia. The high prevalence of intestinal worms in Indonesia allows the occurrence of STH coinfection in pulmonary TB patients which causes anti M. tuberculosis immunity to decrease so that the response to tuberculosis treatment is not optimal. One of the risk factors of STH infection is personal hygiene. The purpose of this study was to analyze the relationship between individual hygiene and the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District. This type of research is observational research with cross sectional analytic design. The population used was TB patients in Tempurejo Sub-District in the period September - October 2019. The data used in this study were primary data in the form of interviews and results of stool examination. Stool examination is carried out using the sedimentation and floatation methods. Data analysis was performed using Fisher's exact test. The results of this study indicate that the incidence of STH co-infection in TB patients in Tempurejo Subdistrict is 9.67% and is caused by two STH species, A. lumbricoides (66.7%) and Hookworm (33.3%). Respondents' personal hygiene consisted of good hygiene (64.5%) and bad hygiene (35.5%), where 66.7% of cases of STH coinfection occurred in respondents with poor personal hygiene. Fisher's test results showed that there was no significant relationship between personal hygiene with the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District (p-value of 0.281).
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Yuna Annisa Salsabila; Enny Suswati; Bagus Hermansyah; Dini Agustina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Indeks adhesi Shigella dysenteriae pada enterosit mencit galur BALB/ pasca pemaparan protein pili 42 kDa Enny Suswati; Dian H. Purnamasari; Desie D. Wisudanti; Diana C; Mufida Mufida
Jurnal Kedokteran Syiah Kuala Vol 20, No 3 (2020): Volume 20 Nomor 3 Desember 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v20i3.18690

Abstract

Abstrak. Tujuan penelitian ini adalah membuktikan bahwa protein pili Shigella dysenteriae  dengan berat molekul 42 kDa merupakan protein adhesi dari S. dysenteriae pada enterosit mencit galur BALB/c. Penelitian ini merupakan penelitian eksperimental laboratoris yang dilaksanakan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Jember, melalui beberapa tahap yaitu isolasi dan identifikasi S. dysenteriae, kultur S. dysenteriae, isolasi pili, SDS-PAGE, pemurnian protein pili, uji hemaglutinasi,isolasi enterosit mencit galur BALB/c, dan uji adhesi. Pada penelitian ini dibentuk 6 kelompok perlakuan dan 1 kontrol negatif. Keenam kelompok perlakuan tersebut meliputi konsentrasi protein pili 1, ½, ¼, 1/8, 1/16, dan 1/32. Data dianalisis menggunakan program statistik SPSS (Statistical Product and Service Solution) versi 16, jenis regresi linier sederhana dan one way Anov. Hasil penelitian menunjukkan bahwa protein pili S. dysenteriae 42 kDa mampu menghambat perlekatan S. dysenteriae terhadap enterosit mencit galur BALB/c. Pada uji regresi linier sederhana diperoleh nilai R square 0,897 yang berarti nilai indeks adhesi dipengaruhi oleh konsentrasi protein pili sebesar 89,7%, dan 10,3% dipengaruhi oleh faktor lain. Hasil uji one way Anova menunjukkan bahwa perbedaan konsentrasi protein pili berpengaruh terhadap indeks adhesi bakteri dengan nilai Sig. = 0,000 (p value 0,05). Kesimpulan yang diperoleh dari penelitian ini sesuai dengan hipotesis, yaitu protein pili dengan berat molekul 42 kDa merupakan protein adhesi dari Shigella dysenteriae pada enterosit mencit galur BALB/c. Kata kunci: Shigella dysenteriae,  protein pili  42 kDa, indeks adhesi Abstract. The aim of this study was to prove that the protein pili Shigella dysenteriae with a molecular weight of 42 kDa is an adhesion protein from S. dysenteriae in enterocytes of BALB / c mice. This research is a laboratory experimental research carried out at the Microbiology Laboratory of the Faculty of Medicine, University of Jember, through several stages, namely the isolation and identification of S. dysenteriae, culture of S. dysenteriae, isolation of pili, SDS-PAGE, purification of pili protein, hemagglutination test, isolation of enterocyte strain mice. BALB / c, and adhesion test. In this study, 6 treatment groups and 1 negative control were formed. The six treatment groups included protein concentrations of pili 1, ½, ¼, 1/8, 1/16, and 1/32. The data obtained were analyzed using the statistical program SPSS (Statistical Product and Service Solution) version 16, simple linear regression and one way Anov. The results showed that the 42 kDa S. dysenteriae pili protein was able to inhibit the attachment of S. dysenteriae to enterocytes of the BALB / c mice. In the simple linear regression test, it was obtained that the R square value was 0.897, which means that the adhesion index value was influenced by the pili protein concentration of 89.7%, and 10.3% was influenced by other factors. The one way Anova test results showed that the difference in pili protein concentration had an effect on the bacterial adhesion index with the Sig. = 0.000 (p value 0.05). The conclusion obtained from this study is in accordance with the hypothesis, namely pili protein with a molecular weight of 42 kDa is the adhesion protein of Shigella dysenteriae in enterocytes of BALB / c mice. Key words: Shigella dysenteriae, 42 kDa pili protein, adhesion index
Uji In Vitro Efek Ekstrak Etanol Biji Kakao (Theobroma cacao) sebagai Antibakteri terhadap Propionibacterium acnes (In Vitro Test of the Effect of Cocoa Beans (Theobroma cacao) Ethanolic Extract as an Antibacterial against Propionibacterium acnes) Ariska Nur Aida; Enny Suswati; Misnawi Misnawi
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Acne vulgaris is a chronic inflammatory disease of the pilosebaceous follicle, characterized by comedones, papules, pustules, cysts and nodules in certain sites of predilection, such as face, neck and back. Propionibacterium acnes are the most recognized bacteria as a key factor for the development of acne. The purpose of this research was to identify the antibacterial effect and Minimum Inhibitory Concentration (MIC) of ethanolic extract of Cocoa beans (T. cacao) against the growth of P. acnes by in vitro method. Antibacterial activity was measured by disk diffusion (Kirby Bauer) method. This was a quasi-experimental with posttest only control group design. Samples used was colony of P. acnes. Concentration of test solution used was 7.81; 15.62; 31.25; 62.50; 125; 250; 500; and 1000 mg/ml. Positive control used clindamycin suspension and negative control used sterile distilled water. By direct measurement, MIC was obtained at the dose of 31.25 mg/ml, by using linier regretion statistical analysis, the quantitatif MIC showed at the dose of 16.93 mg/ml. Analysis using ANOVA showed significance value p=0.000 (p<0.05). In conclusion, ethanolic extract of Cocoa beans had an antibacterial effect on P. acnes by in vitro method.   Keywords: antibacterial effect, cocoa beans, Propionibacterium acnes
Co-Authors Abu Khoiri Aditya Oktavianto Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Alfianti, Nita Angga Mardro Raharjo Anggraini, Nanda Dewi Anisa Rizca Putri APRI KUNTARININGSIH Arifah Nur Hasanah Ariska Nur Aida asihanti rosita ferdiana Azham Purwandhono Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Satrio Pambudi Bagus Satrio Pambudi, Bagus Satrio Cholis Abrori, Cholis Desie D. Wisudanti Desie Dwi Wisudanti Devi Chintya Kumalasari Dian H. Purnamasari Diana C Diana C. Mufida Diana Chusna Mufida Dini Agustina Dwita Aryadina Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga Putri Erfan Efendi Habib Mustofa Haq, Hanifah S I Ketut Sudiana Ika Rahmawati Sutejo Indra Samudra Rahmat Jauhar Firdaus Joko Mariyono Kirana Nadyatara Kristianningrum Dian Sofiana Lailatis Shofia Laksmi Indreswari Lutfadaturroifa, Alya Wafaul M. Ali Shodikin Misnawi Misnawi Misnawi Misnawi Mufida Mufida Mufida, Diana C Muhammad A. Shodikin Muhammad Alif Taryafi Mukarromah, Laylatul Fitriyah Nadhiva, Alvien Zahrotun Nexia Nevarachell Onny Amirsyah Nisrina Salsabila Firmansyah Nita Alfianti Nugraha, Bima Setia Sandya NUR K. AGUSTIN Nurhayati Nurhayati Pratama, Dava Rizky Prayoga Adinawer Sirait Probosutiksna, Nuno Febrian Probosutiksna, Nuno Febrian Putra Uta, Diego Rafi Putu Ayu Laksmi Lestari Raharjo, Angga M Rahmat, Indra Samudra Regina Finka Risty Pradana Linggan Wangi Rumastika, Nindya Shinta Sakinah, Elly N Salsabila, Yuna Annisa Saskia Mediawati septa surya wahyudi, septa surya Shodikin, M. Ali Shofia, Lailatis Sudarmanto, Yohanes Supangat Supangat Supangat Supangat Suryono Suryono Syahputri, Siti Ananda Hardita Ulfa Elfiah Wahyuning Ati Ashari Wiwien Sugih Utami Wiwin Sugih Utami Yudha Nurdian Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Yunita Armiyanti Yunita Wulansari