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The Improvement of Rumen Fermentation Products Through In-Vitro Supplementation of Mg and Co Minerals Suhartati, FM; Suryapratama, Wardhana
ANIMAL PRODUCTION Vol 18, No 2 (2016): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.012 KB) | DOI: 10.20884/1.anprod.2016.18.2.552

Abstract

A study has been conducted to examine the effect of Mg and Co supplementation on rumen fermentation products. The study was conducted in an in vitro experiment, applying completely randomized design, 3x3 factorial. The first factor was three levels of Mg (0; 7.5 and 15.0 mM), and three levels of Co (0; 0.03 and 0.06 mM) as the second factor, total 9 treatments each repeated three times, comprising 27 experimental units all together. In vitro incubation lasted for 4 hours. Variables measured were the concentrations of VFA, N-NH3 and protein synthesis of microbial rumen. Data were subject to analysis of variance and orthogonal polynomials test. The results showed an interaction effect between Mg and Co on the concentration of VFA, N-NH3 and protein synthesis of microbial rumen. The increasing supplementation of Mg at 0.06 mM Co increased VFA concentration; the highest concentration of N-NH3 was achieved by rumen fluid supplemented with 15.0 mM of Mg and 0.03 mM of Co. The highest protein synthesis of microbial rumen was achieved by the rumen fluid supplemented with 8.18 mM of Mg with no supplementation of Co.
The Effects of Branched Chain Volatile Fatty Acids on Reduced Sugar and Branched Chain Amino Acid Concentration of Substrates that Fermented by Aspergillus oryzae Suhartati, FM; Suryapratama, Wardhana
ANIMAL PRODUCTION Vol 4, No 2 (2002): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.188 KB)

Abstract

A study was conducted to know the reduced sugar and branched chain amino acids concentration in substrate that fermented by Aspergillus oryzae. Branched chain amino acids represent amino acids that are very important for microorganism development, including yeast and ruminal microorganism as well as for the growth of the ruminant animal. The study was conducted using Completely Randomized Design (CRD). There were five kinds of supplements that were added into the media. So, that this experiment were A: control, B: A + 0.5% urea, C: B + 1% extract of cassava leaves, D: C + 1% isobutyrate, and E: D + 1.3% 2-methilbutyrate. There were five replicates in each treatment. The measured variables in these study were, colonies cell biomass of A. oryzae, reduced sugar, Crude Protein, and branched chain amino acid concentration. The results showed that the highest number of colonies, concentration of reduce sugar, and concentration of branched chain amino acids was obtain from the substrate of treatments D. (Animal Production 4(2): 83-88 (2002) Key words : Branched Chain Amino Acids, Branched Chain Volatile Fatty Acids, Aspergillus oryzae
Concentration of Partial VFA and Methane Production of Beef Cattle Rument Fluid which Red Dragon Fruit Skin (Hylocereus costaricensis) and Guava Leaf (Psidium guajava L.) in Ammoniated Rice Straw Based Ration Rahmat, Aziz Nur; Suryapratama, Wardhana; Suhartati, FM
ANIMAL PRODUCTION Vol. 22 No. 3 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.702 KB) | DOI: 10.20884/1.jap.2020.22.3.21

Abstract

The research aimed to examine the effect of red dragon fruit skin and guava leaves supplemented into beef cattle feed on the concentration of partial VFA and methane gas production. The in vitro study was conducted in a completely randomized design (CRD) with 3 treatments, namely P0: 50 % concentrate + 50 % ammoniated straw (Control), P1: 50 % concentrate + 50 % ammoniated straw + 5 % red dragon fruit skin flour, and P2: 50 % concentrate + 50 % ammonia straw + 5 % red dragon fruit skin flour + 2.5 % guava leaf flour. The concentrate consisted of 2 parts of rice bran and 1 part of coconut meal. The ratio of concentrat and ammoniated rice straw was 50:50. Each treatment was repeated 6 times. The results showed that the concentration of acetic acid, butyrate and methane gas decreased while the concentration of propionate increased with the addition of red dragon fruit and guava leaves. The research concluded that the addition of 5 % red dragon fruit skin flour combined with 2.5 % guava leaves flour in beef cattle feed could further reduce the concentration of acetic acid, butyric acid and the production of methane gas, but increase the concentration of propionic acid. It is suggested that defaunated agents of red dragon fruit skin and guava leaves could reduce the production of methane gas in the rumen of beef cattle.
Effect of Vitamin A and Magnesium Supplementation on Protein Consumption and Nitrogen Retention of Sheep Tsany, Muhammad Iqbal Rizqi; Suryapratama, Wardhana; Suhartati, Fransisca Maria
JURNAL ILMIAH PETERNAKAN TERPADU Vol 12, No 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p272-286

Abstract

The research aimed to examine the effect of vitamin A and magnesium supplementation on protein consumption and nitrogen retention in sheep was carried out on February 29 - June 26 2023. The material used was 18 local male sheep aged 7 - 8 months with a weight of 17,12 ±1,94 kg were reared for 2 months in individual stage model cages. The basal feed given is concentrate (75%) and ammoniated rice straw (25%). The in vivo experimental method uses a Randomized Complete Block Design (RCBD) as a group, namely the initial weight of the research sheep, consisting of 6 groups that act as repeaters. Treatments tested T1 = Ammoniated rice straw (25%) + concentrate feed (75%); T2 = T1 + vitamin A (2000 IU); T3 = T2 + magnesium mineral (1 gram). The variables measured were protein consumption and nitrogen retention. The data obtained was then analyzed using analysis of variance (ANOVA) which was further tested with the Honestly Significant Difference (HSD) test. Protein consumption was significantly influenced by treatment (P<0.05) with a mean of 101.71 ± 3.85 grams/head/day (T1), 109.03 ± 8.60 grams/head/day (T2), and 115.99 ± 5.35 grams/head/day (T3). Likewise, nitrogen retention was significantly influenced by treatment (T<0.05) with an average of 9.37 ± 1.41 grams/head/day (T1), 9.81 ± 1.51 grams/head/day (T2), 10.21 ± 1.53 grams/head/day (T3). The HSD test results showed that the protein consumption and nitrogen retention of T3 sheep were significantly (P<0.05) higher than those of T1 and T2. The research conclusion was that supplementation of vitamin A and magnesium (T3) in basal feed was able to increase protein consumption by 12.31% and nitrogen retention by 8.24% in sheep when compared to the control treatment (T1).
Effect of Vitamin A and Magnesium Supplementation on Consumption and Digestibility of Dry Matter and Organic Matter in Lamb Jalalludin, Jalalludin; Suryapratama, Wardhana; Prayitno, Caribu Hadi
Jurnal Sain Peternakan Indonesia Vol 20 No 1 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.1.29-33

Abstract

A study to determine the effect of vitamin A and magnesium supplementation on Dry Matter Digestibility (DMD) and Organic Matter Digestibility (OMD) in lamb. The research was conducted experimentally using a Randomized Completely Block Design (RCBD) using 18 rams local aged 7 - 8 months with an average initial body weight of 20 kg ± 2.38 kg.   The feed was given at 4% Dry Matter (DM) of body weight with a ratio of ammoniated rice straw and concentrate of 25%:75%.  The treatments tested were T0 (control), T1 (25% ammoniated rice straw, 75% concentrate, plus 2000 IU of vitamin A), and T2 (25% ammoniated rice straw, 75% concentrate, plus 2000 IU of vitamin A, plus 1 gram of magnesium).  The results of the variance of the analysis showed that the treatment of vitamin A and Magnesium administration had a real effect (p<0.05) on the digestibility of organic matter, while other variables were not accurate. The average digestibility of organic matter was T0 at 91.49%, T1 at 91.19%, and T2 at 92.24%. T2 was the treatment with the highest digestibility, with a difference of 2.75% from the control treatment. After the Randomized Completely Block Design (RCBD) and Variance of Analysis test, the next test used Honestly Significant Difference (HSD). Vitamin A and Magnesium supplementation affected the digestibility of organic matter by 2.75% higher than the control treatment.
PENGARUH PENAMBAHAN TEPUNG DAUN KELOR (Moringa oleifera) DAN MINYAK SAWIT DALAM PAKAN TERHADAP SIFAT FISIK DAGING DOMBA Warastomo, Minggus Tri; Suryapratama, Wardhana; Rahardjo, Agustinus Hantoro Djoko
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1274.577 KB) | DOI: 10.20884/1.angon.2021.3.2.p156-165

Abstract

Background. Research has been conducted to determine the effect of adding Moringa leaf flour (Moringa oleifera) and palm oil in feed on the physical properties of lamb meat. Materials and Methods. The research material used were 18 male thin tail sheep aged 7-8 months with initial body weight 20 ± 2.40 kg and raised 2.5 months. The feed material used were basic feed, namely grass silage and concentrate consisting of 2 parts of rice bran and 1 part of coconut meal, as well as supplementary feed, namely moringa leaf flour and palm oil. The research method was carried out in vivo experimental using a completely randomized design (CRD) with 3 treatments and 6 replications. The treatment given is R0 = basic feed (40% grass silage + 60% concentrate); R1 = R0 + Moringa Leaf Flour 2% of feed BK; and R2 = R1 + Palm Oil 0.5% of feed. The measured variables are pH, cooking losses, tenderness and water holding capacity. Results. The results showed that addition of Moringa leaf flour (Moringa oleifera) and palm oil in feed no significant effect (P> 0.05) against physical properties (pH, cooking losses, tenderness and water holding capacity) sheep's meat. The results of the pH average of the treated lamb R0 6.16 ± 0.27; R1 6.33 ± 0.19; and R2 6.23 ± 0.17. The results of the average cooking loss of treated lamb R0 40.15 ± 1.04%; R1 38.66 ± 2.89%; and R2 39.26 ± 2.34%. The result of flatness of tenderness lamb treatment R0 0.046 ± 0.0027 mm/g/s; R1 0.045 ± 0.0046 mm/g/ s; and R2 0.042 ± 0.0054 mm/g/s, and the results of the average water holding capacity (DIA) lamb treatment R0 42.34 ± 8.30%; R1 44.54 ± 11.19%; and R2 46.50 ± 9.81%. Conclusion. This study can be concluded that the addition of flour Moringa leaf flour (Moringa oleifera) and palm oil in feed have not been able to improve the physical properties (pH, cooking loss, tenderness and water holding capacity) of lamb meat.
PENGARUH PENGGUNAAN TEPUNG DAUN KELOR (MORINGA OLEIFERA) DAN MINYAK KELAPA SAWIT TERHADAP KONSUMSI PROTEIN DAN RETENSI NITROGEN PAKAN DOMBA Qurozi, Abdullah; Suryapratama, Wardhana; Munasik, Munasik
ANGON: Journal of Animal Science and Technology Vol 3 No 3 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (985.878 KB) | DOI: 10.20884/1.angon.2021.3.3.p263-271

Abstract

Background. The purpose of the study was to examine the effect of giving Moringa leaf flour and palm oil on protein consumption and nitrogen retention of lamb diet. Materials and methods. The research was carried out on 27 June 2020 to 27 September 2020, at the Experimental Farm and Nutrition and Animal Feed Science Laboratory, Faculty of Animal Sciene, Jenderal Soedirman University, Purwokerto. The research material used were 18 males thin tailed lamb, aged 7-8 months, the average body weight 18,9 ± 1,9 kg. The feed used were 40% napier grass silage base and 60% concentrate (2 parts of rice bran and 1 part of coconut meal). The supplementary feed given are Moringa leaf flour and palm oil. The study used an in vivo experimental method with a Completely Randomized Design (CRD), 3 treatments and 6 replications. The treatments given was R0 = control feed (40% grass silage + 60% concentrate); R1 = R0 + Moringa leaf flour (2% DM feed); and R2 = R1 + palm oil 0.5% of feed. The variables measured were protein consumption and nitrogen retention of lamb diet. Results. The results showed the of protein consumption at R0= 86.08 ± 8.20; R1= 129.54 ± 18.28; and R2= 109.76 ± 13.60. Average nitrogen retention at R0= 9.63 ± 3.00; R1= 15.99 ± 3.83; dan R2= 13.08 ± 2.02. The results of the analysis showed that the use of Moringa leaf flour (Moringa oleifera) 2% DM feed and palm oil of lamb diet had a very significant effect on lamb protein consumption and a very significant effect on lamb nitrogen retention. Conclusion. The conclusion of the study is the treatment Moringa leaf flour (Moringa oleifera) was able to increase lamb protein onsumption by 50.48%, nitrogen retention in lamb by 66.04%.
the effect PENGARUH PENGGANTIAN KONSENTRAT MENGGUNAKAN AMPAS TEMPE DAN SUPLEMENTASI COMPLETE RUMEN MODIFIER (CRM) TERHADAP KONSUMSI DAN KECERNAAN PROTEIN KASAR PAKAN DOMBA Hutasoit, Irma Uli; Suhartati, Fransisca Maria; Suryapratama, Wardhana
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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Abstract

The study aims to examine the effect of concentrate replacement using tempeh dregs and Complete Rumen Modifier (CRM) supplementation on the consumption and digestibility of crude protein in sheep feed. The study used 18 local male sheep aged 7-8 months with an average weight of 20±2.34 kg. This study used a Randomized Group (RAK) design and used a type of experimental research, as a group as well as a repeat of the initial body weight of sheep after the adaptation period as many as 6 groups and 3 treatments, including P1: Ammoniated rice straw ad libitum + concentrate 2.5% DM of body weight (control), P2: Ammoniated rice straw ad libitum + tempeh dregs 2.5% DM of body weight, and P3: Ammoniated rice straw ad libitum + tempeh dregs 2.5% DM of body weight + 1% CRM. The variables observed in the study were consumption and digestibility of crude protein. Based on the results of the analysis of treatment variance, the effect was very real (P<0.01) on crude protein consumption with an average consumption of P1, P2, P3 treatments, respectively 10.26±0.05; 14.35±0.78; 14.02±0.88 (g/head/d) and a significant effect (P<0.005) on digestibility with crude protein digestibility rate of P1, P2, P3 treatment respectively at 59.44±3.65%; 67.85±3.44%; 63.56±4.83%. Based on orthogonal contrast tests, tempeh dregs can replace concentrate feed entirely and even increase crude protein consumption and digestibility by 3.90% and 3.93% respectively without 1% CRM supplementation. Keywords: CRM, tempe dregs, lamb, crude protein, consumption, digestibility.
Effect Effect of Different Forages and Concentrates Balance in Complete Feeds on In Vitro the Digestibility of Crude Fat and Extractable Material Without Nitrogen Ridhasmara, Arum Pidie; Suryapratama, Wardhana; Susanti, Emmy
ANGON: Journal of Animal Science and Technology Vol 6 No 2 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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Abstract

ARUM PIDIE RIDHASMARA. The aim of the study was to examine the effect of different forage and concentrate balances on crude fat digestibility and BETN in vitro. Experimental research using a completely randomized design (CRD) with 3 treatments and 6 replicates. The treatments consisted of P1: 30% forage + 70% concentrate + 0.01 organic chromium; P2: 50% forage + 50% concentrate + 0.01 organic chromium; P3: 70% forage + 30% concentrate + 0.01 organic chromium. The results of the analysis showed that the digestibility of crude fat in P1 (30% forage and 70% concentrate) with P2 (50% forage and 50% concentrate) was not significantly different compared to P3 (70% forage and 30% concentrate) but P1 with P3 showed a significant difference (P <0.05). The analysis showed that BETN digestibility in P1 (30% forage and 70% concentrate) with P2 (50% forage and 50% concentrate) showed no significant difference. P2 with P3 (70% forage and 30% concentrate) showed a significant difference (P<0.01), while P1 with P3 showed a very significant difference (P<0.01). In conclusion, the test results proved that every decrease, the digestibility value of crude fat will increase and the BETN digestibility value of material will be smaller
KECERNAAN BAHAN KERING DAN BAHAN ORGANIK PAKAN DOMBA YANG DIBERI DAUN KELOR (Moringa oleifera) DAN MINYAK SAWIT SECARA IN VITRO NUGROHO, TRI AJI; Suryapratama, Wardhana; Munasik, Munasik
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2024.6.3.p237-242

Abstract

Latar Belakang. Suatu Penelitian telah dilaksanakan untuk mengkaji pengaruh pemberian daun kelor dan minyak sawit terhadap kecernaan bahan kering dan bahan organik secara in vitro. Penelitian dilaksanakan pada 27 Juni 2020 sampai dengan 12 September 2020. Materi dan Metode. Materi yang digunakan dalam penelitian adalah cairan rumen 3 ekor domba yang diperoleh sesaat setelah pemotongan, dan sebelumnya domba telah diberi pakan dengan perlakuan yang sama dengan penelitian. Ransum perlakuan yang digunakan terdiri atas R0 = Pakan kontrol tanpa daun kelor dan minyak sawit, R1 = Pakan kontrol + 2% tepung daun kelor dari bahan kering (BK) pakan, dan R2 = Pakan kontrol + 2% tepung daun kelor dari BK pakan + 0,5% minyak sawit dari BK pakan. Penelitian dilakukan secara in vitro, variabel yang diukur yaitu Kecernaan Bahan Kering dan Bahan Organik. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh nyata (P<0,05) terhadap kecernaan bahan kering dan bahan organik. Simpulan. Disimpulkan bahwa campuran suplementasi 2% tepung daun kelor dan 0,5% minyak sawit mampu meningkatkan kecernaan bahan kering pakan domba sebesar 4,1 % dan kecernaan bahan organik pakan domba 3,2 %.