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<![CDATA[EKSPLORASI DAN KARAKTERISASI ENTOMOPATOGEN ASAL BERBAGAI INANG DAN LOKASI [Exploration and Characterization of Entomopathogenic from Various Host and Location] ]]>
<![CDATA[Priyatno, Tri Puji]]>;
<![CDATA[Samudra, I Made]]>;
<![CDATA[Manzila, Ifa]]>;
<![CDATA[Susilowati, Dwi Ningsih]]>;
<![CDATA[Suryadi, Yadi]]>
<![CDATA[ BERITA BIOLOGI Vol 15, No 1 (2016) ]]>
Publisher : <![CDATA[Research Center for Biology-Indonesian Institute of Sciences ]]>
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DOI: 10.14203/beritabiologi.v15i1.2859
<![CDATA[Microbial groups of entomopathogenic (fungi and bacteria) had been reported causing insect mortality. The aim of the study was to explore and characterized entomopathogenic from various host and locations. Fungal identification at genus and species level was caried out based on conidial morphology, hyphal growth, conidiophore and colony color; whilst for bacterial identification was based on standard Bergeyâs manual for determinative bacteria. Sixteen entomopathogenic isolates that consisted of fungal and bacteria have been collected and preserved for further characterization. Of the 16 entomopathogen collected samples, five fungal genera was found i.e. Paecilomyces; Metarhizium, Beauveria, Hirsutella; and Cordyceps. Seven isolates belonging to six fungal isolates, and one bacterial isolate had been identified based upon ITS and 16S rDNA sequences, respectively. We confirmed that 6 fungal isolates belong to species of Paecilomyces reniformis, B. bassiana, M. anisopliae, M. anisopliae var acridum, Hirsutella thomsonii. One isolate of red pigmented bacteria Sm201102 have been identified was belonging to species Seratia marcescence. It was also obtained two fungal isolates from different host (spider and beetle) which confirmed by morphological character belong to Cordyceps sp. ]]>
<![CDATA[PATOGENISITAS ISOLAT BAKTERI Xanthomonas oryzae pv. oryzae DAN PEMANTAUAN PENYAKIT HAWAR DAUN BAKTERI PADA PADI GALUR ISOGENIK [Pathogenicity of Xanthomonas oryzae pv. oryzae Isolates and Bacterial Leaf Blight Disease Monitoring on Rice-Near Isogenic Lines (NILs)] ]]>
<![CDATA[Suryadi, Yadi]]>;
<![CDATA[Kadir, Triny Suryani]]>
<![CDATA[ BERITA BIOLOGI Vol 16, No 2 (2017) ]]>
Publisher : <![CDATA[Research Center for Biology-Indonesian Institute of Sciences ]]>
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DOI: 10.14203/beritabiologi.v16i2.2393
<![CDATA[Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease due to its high intensity that and  the mereased durability of its resistant variety. This study was aimed  to determine the pathogenicity of Xoo isolates, which obtained from four regions/districts in West Java on three rice near isogenic lines (NILs) containing a single resistance (R) gene; and  to study reaction of rice NILs/ differential genotypes containing mixture of resistant genes to Xoo population under endemic areas. Out of 22 Xoo isolates have been collected and further determined by ELISA assay. Ten Xoo isolates were selected and inoculated to identify their pathogenicity on three NILs i.e., IRBB5 (xa5), IRBB7 (Xa7) and IRBB21 (Xa21). Pathogenicity test showed that most of isolates produced large lesion, and four virulence groups were identified. Phylogenetic analysis suggested that the dominant virulent isolates were widely distributed at several district in West Java. It was shown that 11 NILs exhibited high levels of resistant reaction to the predominant Xoo pathotypes in Cianjur, while 10 lines were susceptible. The single R gene (IRBB7, IRBB21 and Java14) exhibited lower BLB severity. The IRBB50, IRBB51, IRBB52, IRBB53 lines (carrying two R genes), IRBB56, IRBB57 (carrying three R genes); IRBB64 (carrying four R genes), and IRBB66 (carrying five R genes) showed lower severity and thus produced higher resistance to Xoo. This study may implied further work to deploy effective R genes against certain Xoo pathotypes in differ  region.   ]]>
<![CDATA[SELEKSI JAMUR PATOGEN SERANGGA Beauveria spp. SERTA UJI PATOGENISITASNYA PADA SERANGGA INANG-WALANG (Leptocorisa acuta) [Selection of Enthomopathogenic Fungi Beauveria spp. and their Pathogenicity Test Against Insect Host-Rice Stink Bug (Leptocorisa acuta)] ]]>
<![CDATA[Wartono, Wartono]]>;
<![CDATA[Nirmalasari, Cyntia]]>;
<![CDATA[Suryadi, Yadi]]>
<![CDATA[ BERITA BIOLOGI Vol 15, No 2 (2016) ]]>
Publisher : <![CDATA[Research Center for Biology-Indonesian Institute of Sciences ]]>
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DOI: 10.14203/beritabiologi.v15i2.2271
<![CDATA[Rice-stink bug (Leptocorisa acuta Thumb) that attack rice crop often causing heavy damage of panicle of rice as well as decreasing either quantity or quality of grain after harvest. The objective of this research was to select 14 entomopatogenic fungi of Beauveria spp. isolates, collected from rice stink bug (L. acuta) and their pathogenicity assay to insect host L. acuta. We also aimed to study diversity of Beuveria spp. which was isolated from insect host from Situgede, Bogor. The research was conducted at Laboratory, and Green house of Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, in 2014. Result of the study showed that isolates from Situgede Bogor were sucessfully isolated  as Beauveria spp. based on Koch Postulat assay.  The identified fourteen isolates were morphologically confirmed as Beauveria spp. The pathogenicity test was indicated by symptoms and mortality of rice stink bug after inoculation with these entomopathogenic fungi. The Beuveria isolates Stgd2(14)1, Stgd6(14)1, Stgd7(14)2, Stgd8(14)2, and Stgd0113 were the most virulent isolates. Stgd2(14)1 provided the fastest time to kill L. acuta with LT50 values of 6.9 days. The 13 isolates of Beauveria were well amplified by ITS primers. However, no diversity was found among isolates, presumably due its narrow host range tested. ]]>
<![CDATA[PENDETEKSIAN BAKTERI Raistonia solanacearum, Yabuuchi et al 1995 MENGGUNAKAN TEKNIK REAKSI POLIMERASE BERANTAI DAN PEMBEDAAN STRAIN MENGGUNAKAN TEKNIK HIBRIDISASI DNA ]]>
<![CDATA[Suryadi, Yadi]]>;
<![CDATA[Machmud, M]]>;
<![CDATA[Suhendar, MA]]>
<![CDATA[ BERITA BIOLOGI Vol 5, No 1 (2000) ]]>
Publisher : <![CDATA[Research Center for Biology-Indonesian Institute of Sciences ]]>
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DOI: 10.14203/beritabiologi.v5i1.1090
<![CDATA[Raistonia solanacearum,the bacterial wilt pathogen, has a wide host range and genetic variability.Rapid and sensitive molecular techniques need to be developed for eariy detection and strain differentiation of the pathogen.Molecular techniques such as PCR and DNA hybridization have been succesfully used to detect and identify bacterial plant pathogens including R.solanacearum.These techniques were adopted under Indonesian condition, using purified and crude DNA from infected plant samples.An R.solanacearum specific DNA primer (OH/Y2) was used in the PCR test,and a DNA probe 5a67 were used in the non-radioactive hybridization test.The PCR techniqe could be used to detect R.solanacearum from infected plant samples in less than 5 hours.The DNA hybridization technique was applicable to differentiate strains ofR.solanacearum into three groups based on their DNA profiles.]]>
<![CDATA[KARAKTERISASI BAKTERI PENGHASIL ASAM INDOL ASETAT DAN PENGARUHNYA TERHADAP VIGOR BENIH PADI ]]>
<![CDATA[Lestari, Puji]]>;
<![CDATA[Suryadi, Yadi]]>;
<![CDATA[Susilowati, Dwi Ningsih]]>;
<![CDATA[Priyatno, Tri Puji]]>;
<![CDATA[Samudra, I Made]]>
<![CDATA[ BERITA BIOLOGI Vol 14, No 1 (2015) ]]>
Publisher : <![CDATA[Research Center for Biology-Indonesian Institute of Sciences ]]>
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DOI: 10.14203/beritabiologi.v14i1.1859
<![CDATA[The ability to produce indole acetic acid (IAA) by endophytic bacteria is one of the basic criteria for the use of bacteria as plant growth promoter agent which is essential for the agricultural production.The objectives of this study were to evaluate the ability of 17 bacterial isolates to produce IAA and its effect on improvement of rice seed germination and molecular identification of the selected isolates based on the 16S rRNA gene. The IAA content was determined using Salkowski method measured by spectrophotometer UV-Vis and the effect of endophytic bacteria inoculation on seed germination was done by in vitro assay. Sequences of the selected isolates 16S rRNA amplified by PCR were analyzed the homology against bacterial 16S rRNA database in Genebank. IAA values ranged from 6.632 to 50.053 mg/L with the highest IAA production shown by isolate 6KJ which was followed by 4PB (41.807 mg/L). Bacterial IAA increased rice seed vigor significantly compared to control. However, bacterial inoculation with different concentrations of IAA did not significantly affect the growth of rice plants. Based on the IAA and its effect on seed vigor, 6KJ, 4PB and 2KB were selected for molecular identification. Results showed that the three isolates belonged to Bacillus genus, 6KJ as B. aryabhattai, 4PB belonging to B. cibi and 2KB having 97% homology with B. marisflavi. Further evaluation of the selected endophytic isolates producing IAA is necessary to be carried out to explore their potency as a source of hormone to promote plant growth.]]>
<![CDATA[KARAKTERISASI ß-1,3-1,4-GLUKANASEBAKTERI ENDOFITIK BURKHOLDERIA CEPACIA ISOLATE76 ASAL TANAMAN PADI ]]>
<![CDATA[Manzila, Ifa]]>;
<![CDATA[Priyatno, Tri Puji]]>;
<![CDATA[Fathin, Muhammad Faris]]>;
<![CDATA[Ambarsari, Laksmi]]>;
<![CDATA[Suryadi, Yadi]]>;
<![CDATA[Samudera, I Made]]>;
<![CDATA[Susilowati, Dwi Ningsih]]>
<![CDATA[ BERITA BIOLOGI Vol 14, No 2 (2015) ]]>
Publisher : <![CDATA[Research Center for Biology-Indonesian Institute of Sciences ]]>
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DOI: 10.14203/beritabiologi.v14i2.1819
<![CDATA[Pathogenic fungus is one of the constraints to increase crop production. Chemical control using fungicides caused negative effects either to the environment or increased pathogen resistance to fungicide. Biological control using microbial-producing ß glucanase is an alternative method to inhibit the growth of pathogenic fungus. The aim of this study was to characterize ß-1,3-1,4-glucanase produced by rice endophytic bacterium, B. cepacia E76. Purification was carried out by ammonium sulphate precipitation, dialysis, and ion exchange chromatography using DEAE sepharose Fast Flow. A further characteristic of the enzyme activity was studied using oatmeal-glucan substrate.Results showed that precipitation using saturated 80% ammonium sulphate generated a good yield with the purity increased by 11 fold and yield of 66%.After chromatography step, the ß-1,3-1,4-glucanase of B. cepacia was successfully purified with an increasedof purity up to 33 fold and yield of 4%. Based on 10% SDS-PAGE, the enzyme profiles had the molecular weight of 15, 48 and 55 kDa.Of the three isozymes, only the 48 kDa isozyme showed the strongest glucanase activity when grown on media containing glucan as substrate.]]>
<![CDATA[PATOGENISITAS Beauveria bassiana STRAIN STGD 7(14)2 DAN STGD 5(14)2 TERHADAP WERENG COKLAT (Nilaparvata lugens STÃ…L) ]]>
<![CDATA[Suryadi, Yadi]]>;
<![CDATA[Wartono, Wartono]]>;
<![CDATA[Susilowati, Dwi Ningsih]]>;
<![CDATA[Lestari, Puji]]>;
<![CDATA[Nirmalasari, Cyntia]]>;
<![CDATA[Suryani, Suryani]]>
<![CDATA[ Al-Kauniyah: Jurnal Biologi Vol 11, No 2 (2018): Al-Kauniyah Jurnal Biologi ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami ]]>
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DOI: 10.15408/kauniyah.v11i2.6694
<![CDATA[AbstrakBeauveria spp. merupakan salah satu jamur entomopatogen yang dapat digunakan sebagai agen biokontrol terhadap berbagai serangga hama. Tujuan penelitian ini adalah untuk menguji patogenitas Beauveria spp. terhadap serangga wereng coklat (Nilaparvata lugens Stål). Isolat-isolat Beauveria spp. diisolasi dari serangga walang sangit dari Situ Gede, Bogor, Jawa Barat. Identifikasi jamur dilakukan berdasarkan analisis data sekuen dari daerah Internal Transcribed Spacer (ITS) rDNA. Hasil uji menunjukkan bahwa patogenisitas isolat Stgd 5(14)2 dan Stgd 7(14)2 menghasilkan tingkat mortalitas 100% terhadap N. lugens. Isolat Stgd 5(14)2 dan Stgd 7(14)2 memiliki nilai LT50 yang rendah, danmenyebabkan kematian yang cepat terhadap wereng coklat. Hasil analisis filogenetik menunjukkan bahwa sekuen Stgd 5(14)2 dan Stgd 7(14)2 termasuk ke dalam spesies Beauveria bassiana s.str. Hasil studi ini merupakan kajian awal terhadap patogenisitas jamur entomopatogen B. bassiana terhadap wereng coklat, dan distribusinya di pertanaman padi Situ Gede, Jawa Barat.Abstract Beauveria spp. is one of the entomopathogenic fungi that can be used as biocontrol agents against various insect pests, including brown planthopper (Nilaparvata lugens Stal). This study aimed to test the pathogenicity of Beauveria spp. against N. lugens. Beauveria spp. were isolated from the rice stink bug insects, collected from Situ Gede, Bogor, West Java. Fungal identification was carried out based on the internal transcribed spacer (ITS) rDNA analysis. The pathogenicity assay revealed that Stgd 5(14)1 and Stgd 7(14)2 isolates were virulent against N. lugens, with a mortality of 100%. The LT50 (median lethal time) determination, indicated that Stgd 5(14)2 and Stgd 7(14)2 isolates had low value indicating a faster mortality in brown planthopper insects. Phylogenetic analysis showed that the sequences of Stgd 5(14)2 and Stgd 7(14)2 belong to Beauveria bassiana s.str. This is the preliminary pathogenicity trial of entomopathogenic fungi B. bassiana against brown planthopper and their distribution in rice-growing, Situ Gede area in West Java.]]>
<![CDATA[KEEFEKTIFAN FORMULASI BAKTERI BURKHOLDERIA CEPACIA ISOLAT E76 TERHADAP RHIZOCTONIA SOLANI KüHN PADA PERTUMBUHAN TANAMAN PADI DI LABORATORIUM ]]>
<![CDATA[Wartono, Wartono]]>;
<![CDATA[Suryadi, Yadi]]>;
<![CDATA[Susilowati, Dwi Ningsih]]>
<![CDATA[ JURNAL AGROTROPIKA Vol 17, No 2 (2012): Agrotropika Vol.17 No.2 2012 ]]>
Publisher : <![CDATA[JURNAL AGROTROPIKA ]]>
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<![CDATA[In vitro study was carried out to test the effectiveness of the formulation containing Burkholderia cepacia isolates E76 in suppressing growth of Rhizoctonia solani and its effect on plant growth. The study was conducted at Plant Disease Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor, in January to July 2012. The in vitro method was done by growing the bacteria B. cepacia isolates E76 together with R. solani in one dish of potato dextrose agar (PDA) medium. Seed of ?Ciherang? rice was treated with formulation of B. cepacia isolates E76. Observation were done to relative inhibitory rate, rice germination, shoot length, and root length. Results showed that B. cepacia isolates E76 formulation was effective in suppressing the growth of R. solani with relative inhibitory rate at 24 and 48 hour after incubation, respectively 31,3 ? 60,2% and 28,9 ? 47,8%. Rice germination and growth on treated rice seeds were better than control treatment. Concentration at 3% concentration was suggested to be used as recommended concentration for further testing. Key words: Formulation, Burkholderia cepacia isolat E76, Rhizoctonia solani]]>
<![CDATA[HUBUNGAN AKTIVITAS OLAHRAGA DENGAN KEJADIAN HIPERTENSI PADA KELOMPOK USIA 20-40 TAHUN DI DESA WILANAGARA TAHUN 2019 ]]>
<![CDATA[Suryadi, Yadi]]>
<![CDATA[ Jurnal Ilmiah Dozen Globalindo Vol. 1 No. 3 (2021): January ]]>
Publisher : <![CDATA[Kos Globalindo Group ]]>
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<![CDATA[The problem in this study is that hypertension in Wilanegara village is very high. The formulation of the problem in this study is the extent of the relationship of sports activities with the incidence of hypertension in the age group of 20-40 years in Wilanagara Village in 2019 ?. The purpose of this study was to determine the relationship of sports activities with the incidence of hypertension in the age group of 20-40 years in Wilanagara Village in 2019. The method used in this study was descriptive correlational. The population in this study was hypertension sufferers in the age group of 20-40 years in Wilanagara Village in 2019, amounting to 528 people. Determination of the number of samples using the Slovin formula is a 10% significance level of 84 respondents. The selection of 84 respondents used a random sampling approach. Data processing techniques used are correlation test, simple linear regression test and hypothesis testing. The results of the validity of the variable X questionnaire score with variable Y showed 20 questionnaires declared valid. The reliability results showed that the X and Y variable questionnaires were stated to be reliable. Sports activity in the age group of 20-40 years in Wilanagara Village in 2019 has reached 76%, this is included in the high criteria. the incidence of hypertension in the 20-40 years age group in Wilanagara Village in 2019 has reached 32%, this is included in the low criteria. Based on the results of the calculation of the coefficient test results can be explained that the value of tcount> ttable, namely 4.188> 2.015 with a significance level of 0.008 <0.05, meaning that statistically Ha is accepted, meaning that there is a relationship between sports activity and the incidence of hypertension in the age group of 20-40 years in Wilanagara Village in 2019. The conclusion in this study is that there is a relationship between sports activities and the incidence of hypertension in the age group of 20-40 years in Wilanagara Village in 2019.]]>
<![CDATA[PENGARUH PENGETAHUAN TERHADAP PERILAKU MEROKOK PADA SISWA PUTRA KELAS XI DI SMK JAGARA KECAMATAN DARMA KABUPATEN KUNINGAN TAHUN 2019 ]]>
<![CDATA[Suryadi, Yadi]]>
<![CDATA[ Jurnal Ilmiah Dozen Globalindo Vol. 1 No. 3 (2021): January ]]>
Publisher : <![CDATA[Kos Globalindo Group ]]>
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<![CDATA[This research is backed by a thought that now there is an increase in the active smoker on the teenager (aged 15-19 years) that is from 5% to 17% (Depkes, 2013). All of the health expert have been concluded that in health, smoking would give the negative effect, especially for their children. Now smoking in the teenagers (aged 15-19) or same as SMA/SMK is a behavior that is very easy to be founded. All of the health expert have been concluded that in health, smoking would give the negative effect, especially for their children. Now smoking in the teenagers (aged 15-19) or same as SMA/SMK is a behavior that is very easy to be founded. Teen smoker in public places and not infrequently they are smoking by using uniform and are close with the school. One of the factor that affects the behavior smoking is low of knowledge The purpose of this research is to know the knowledge and the smoking behavior of students in the XI grade men students at SMK JAGARA. The type of this research is quantitative research with the design of descriptive analysis design correlation with cross sectional approaches. The sample of this research is 70 students of XI grade son in SMK JAGARA. With saturation sampling method. Retrieval of quantitative data is by giving quessionnaires. The tests conducted by validity test shows that all of data are valid, then reliability test shows that variable X (0.832) and variable Y (0.713) so it’s reliable. The linearity test can be known that the value is contstant as much as 50.607 and X is 0.509 then it’s in direct because there is the influence between variable X and variable Y, normality test of variable X obtained the signify value as much as 0.341 and variable Y as much as 0.427 where all of them are normally distributed, then the hyphothesis test shows that Ha is accepted and Ho is rejected with the result shows the percentage of the influence of knowledge towards smoking behaior as much as 46,6%. Description analysis shows that student’s knowledge is high and smoking behavior is moderate. It’s would be concluded that there’s the influence of knowledge towards smoking behavior.]]>
