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SUSCEPTIBILITY OF RIFAMPICIN-ISONIAZID RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES AGAINST LEVOFLOXACIN Kurniawan, Alvin Hartanto; Mertaniasih, Ni Made; Soedarsono, S.
BALI MEDICAL JOURNAL Vol 5 No 1 (2016)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Tuberculosis (TB) is a high burden disease in Indonesia with multidrug-resistant (MDR) TB incidence started to increase. Treatment success of MDR-TB globally was low in number than it was targeted which was especially caused by fluoroquinolone resistance. One of the fluoroquinolone is levofloxacin, an antibiotic that has been widely used irrationally as antimicrobial treatment. Therefore, this study investigated the sensitivity and MBC of MDR Mycobacterium tuberculosis isolates against Levofloxacin. Method: The susceptibility test for MDR -Mycobacterium tuberculosis on levofloxacin by standard method with levofloxacin were on concentrations 0,5 ?g/ml, 1 ?g/ml, and 2 ?g/ml. Sample of 8 strains MDR-Mycobacterium tuberculosis were cultured with each concentrations on Middlebrook 7H9 for 1 week incubation. Next, each of the incubated concentration was subcultured on solid media Middlebrook 7H10 for 3 weeks incubation. Colonized agar plates after 3 weeks incubation were confirmed with acid-fast stain. Results: On MB 7H10 with levofloxacin concentration 2 ?g/ml showed bactericidal effect 100% by no MDR Mycobacterium tuberculosis colony grew (0/8) while the MB 7H10 with levofloxacin concentration 1 ?g/ml and 0,5 ?g/ml showed the bactericidal effect 37,5% and 25% respectively. The colonized agar plate implied that the MDR Mycobacterium tuberculosis with levofloxacin concentration 1 ?g/ml (5/8) and 0,5 ?g/ml (6/8) grew well. Conclusion: Levofloxacin concentration 2 ?g/ml was susceptible on MDR Mycobacterium tuberculosis. The concentration 2 ?g/ml of levofloxacin could be considered as MBC.
SPECIFIC GYRB SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATED FROM SPUTUM OF PULMONARY TUBERCULOSIS PATIENTS IN INDONESIA Mertaniasih, Ni Made; Wiqoyah, Nurul; Kusumaningrum, Deby; Soedarsono, S.; Perwitasari, Agnes Dwi Sis; Artama, Wayan Tunas
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Indonesia have many different geographic areas which could be various on the variant strains of Mycobacterium tuberculosis. The gyrB gene codes GyrB protein as sub unit compound of Gyrase enzyme that functioning in multiplication of bacteria. Detection of gyrB gene could be a marker of active multiplication of viable bacteria in the specimen from patients; and some of the DNA sequence regions were conserved and specific in the strain of Mycobacterium tuberculosis that would be a marker for identification. This research aims to analyze the sequence of gyrB gene of Mycobacterium tuberculosis clinical isolates from sputum of pulmonary TB patients in Indonesia, and determine the specific region. Method: Mycobacterium tuberculosis clinical isolates have been collected from sputum of the patients with pulmonary TB that live in some area in Indonesia. Isolation and identification of Mycobacterium tuberculosis clinical isolates using standard culture method; sequence analysis using PCR-direct sequencing of the part bases region of gyrB. Results: this study revealed that nucleotide sequence on a fragment 764 bases of gyrB gene Mycobacterium tuberculosis strains among clinical isolates almost identically to a wild type strain Mycobacterium tuberculosis H37Rv and subspecies member of Mycobacterium tuberculosis complex (MTBC), with a little difference of SNPs; there are many difference nucleotide sequence with MOTT and Gram positive or negative bacteria, except Corynebacterium diphtheria identically with MTBC. Conclusion: the gyrB sequence in Mycobacterium tuberculosis strains among these clinical isolates from sputum of pulmonary TB patients in Indonesia have the conserved specific DNA region that almost identically with wild type strain H37Rv and MTBC.
THE EFFECT OF DENSITY AND FLOOR TYPES ON PERFORMANCE, PHYSIOLOGICAL STATE AND IMMUNE RESPONSE OF BROILERS Sunarti, D.; Haryono, H.; Soedarsono, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 4 (2010): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.4.275-281

Abstract

The purpose of this research was to study the effect of density and floor types on performance,physiological state and immune response of broilers. The research involved 368 male broilers of theNew Lohman strain aged 8 days which were raised up to 35 days at different densities and floor types.Floor types consisted of rice hull litter and bamboo slat were used as the main plot; while densities of 7,10, 13 and 16 birds/m2 applied as the sub-plot. The results showed that the final body weight gain of the35-day Lohmann broilers at densities of 7, 10 and 13 birds/m2 were 28.22, 24.43 and 19.27 kgrespectively, compared to 16 birds/m2 at 13.53 kg (P>0.05). Broilers in the bamboo slats floor hadlymph weight at 3.73 g compared to the litter floor at 2.55 g (P<0.05). Also, broilers in the bambooslats had average RHL (0.65) lower than broilers in the litter floor (0.79). It could be concluded thatbamboo slats best being used for broilers up to a density of 13 broilers/m2.
Discordance between Genexpert, Line Probe Assay and Drug Susceptibility Test in Assessing Drug-Resistant Tuberculosis Tutik Kusmiati1 , Kristin Purnama Dewi2 , Yulia Devina Suci2 , Soedarsono3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13642

Abstract

Background: Rapid molecular diagnostics have potentially revolutionized early detection of drug-resistanttuberculosis (DR-TB) in Indonesia. However, there is discordance between conventional culture usingdrug susceptibility test (DST) and rapid diagnostic tools using GeneXpert and line probe assay (LPA).This discordance result can cause confusion to clinician in determining diagnosis of DR-TB. Objective:This study aimed to identify discordance between GeneXpert, LPA, and DST. Methods: A retrospectivestudy was conducted at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. Data were collectedbased on medical record between third to fourth quarter of 2018. Rifampicin resistant-tuberculosis (RR-TB)proven by GeneXpert, were further analyzed with second-line LPA and DST. Discordance result betweenit was analyzed using McNemar. Results: Among 81 patients diagnosed with DR-TB, 59 patients RR-TBwere eligible in this study. There were 13 (22.0%) DST rifampicin result showed sensitive but resistantto GeneXpert. Among 53 samples from LPA, there were 3 (5.7%) result showed LPA fluoroquinoloneresistant but sensitive to DST ofloxacin; 10 (18.9%) result has LPA fluoroquinolone sensitive but resistantto DST ofloxacin; 2 (3.8%) has LPA second-line injectable drug (LPA SLID) resistant but sensitive to DSTkanamycin; 5(9.4%) has LPA SLID sensitive but resistant to DST kanamycin. The McNemar analysis showeddiscordance between GeneXpert and DST rifampicin was 13 (22.0%; p=0.046); LPA fluoroquinolone andDST ofloxacin was 13 (24.6%; p=0.042); LPA SLID and DST kanamycin was 7 (13.2%; p=0.183). Novariable that can be used to analyze discordance result between GeneXpert and LPA. Conclusion: Thereis significant discordance between GeneXpert and DST; LPA fluoroquinolone and DST ofloxacin, whileneither LPA SLID nor DST kanamycin shows no significant discordance.
A Woman with Tuberculosis Multidrug Resistance and QTc Prolongation Repetitive Interval: A Case Report Tutik Kusmiati; Ni Made Mertaniasih; Johanes Nugroho Eko Putranto; Budi Suprapti; Soedarsono; Abdul Rahman Bahmid
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17119

Abstract

Background : Prolonged QTc interval is one of the side effects of the short-regimen MDR-TB. QTcprolongation is a risk factor for Torsades de pointes and is influenced by many factors. Case : 47-year-oldwoman who was diagnosed with MDR-TB through GeneXpert examination and received short-regimenMDR-TB. This patient experienced repeated QTc prolongation, with peak QTc interval 600 msec occurringat 4th month with mild hypokalemia without clinical symptoms. The patient completed 9 months of shortregimentherapy with improve chest x ray followed by negative sputum culture. Conclusion : Short-regimenMDR-TB contains several drugs that cause QTc prolongation. Clinical evaluation is required in patientswith QTc prolongation before changing the regiment.
Pengaruh Psikoedukasi Audio Visual Berbasis Implementation Intention Terhadap Niat dan Perilaku Kepatuhan Minum Obat Pasien Tuberkulosis (TB) di Wilayah Kerja Dinas Kesehatan Kabupaten Tulungagung Anita Dewi Anggraini; Soedarsono Soedarsono; Laily Hidayati
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 10, No 4 (2019): Oktober 2019
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf10411

Abstract

Introduction: Treatment failure, disease transmission and drug resistance were problems that occur due to non-compliance. One of the factors caused non-compliance was a prolonged psychological problem and a lack of patient commitment to treatment. Psychoeducation which was guided by implementation intention and packaged in audio-visual form was expected to improve intention and medication adherence behaviour. Aims: This study aims to explain the effect of implementation intention based audiovisual psychoeducation on intention and taking medicine adherence behaviour of TB patients in the Tulungagung District Health Office. Methods: The research method used was a quasi-experimental design with a pre-post control group. The study sample was 72 TB patients who underwent intensive category 1 treatment as many as 2 divided into 2 intervention groups (n=36) and controls (n=36). Samples were taken by cluster random sampling, inclusion criteria for intensive treatment category 1 TB patients (1-2 months), aged 18-65 years and able to communicate verbally and in writing well. The intention of taking medicine was assessed using a questionnaire about intentions developed from Sari et all research, while taking medicine adhererence behaviour was assessed using the Morisky Medication Adherence Scale (MMAS-8) questionnaire which has been developed by Mahayu et al. Data was analyzed using the Wilcoxon-marked ranking test and Mann-Whitney test. Results: After audio visual psychoeducation intervention based on implementation intention, it was found that there was a significant increase in intetion and taking medicine adherence behaviour in the intervention group with P
EFFECT OF COMBINATION PURSED LIP BREATHING AND GUIDED IMAGERY MUSIC ON PEAK EXPIRATORY FLOW PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE Rudi Hariyono; Soedarsono Soedarsono; Makhfudli Makhfudli
Jurnal Keperawatan Vol. 10 No. 1 (2019): JANUARI
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.999 KB) | DOI: 10.22219/jk.v10i1.6353

Abstract

ABSTRACTBackground: Peak expiratory flow influenced by several things, such as anxiety, a psychological factor that is becoming a major comorbidities of COPD and affect the occurrence of exacerbations, increase respiration rate, duration expiratory time, and hyperinflation. This study aimed to determine the effect of combination pursed lip breathing and guided imagery music to increase  value of peak expiratory flow in patients with COPD. Methods: The study was quasi-experimental design with pretest posttest with control group approach, respondents of this study is 46 patients with COPD with sampling technique, simple random sampling. Results: Pursed lip breathing increase peak expiratory flow values by the significant of p value = 0.000 (p <0.05). The combination of pursed lip breathing and guided imagery music increase peak expiratory flow values by the significant of p value = 0.000 (p <0.05). COPD patients who received a combination of pursed lip breathing and guided imagery music increased peak expiratory flow value higher than the pursed lip breathing without combination with significant of p value = 0.000 (p <0.05).Conclusion:The combination of pursed lip breathing and guided imagery music proved to have an effect on increasing the value of peak expiratory flow higher than pursed lip breathing without combination.
THE CORRELATION BETWEEN SEX, AGE, NUTRITIONAL STATUS AND DIABETES MELLITUS WITH CLINICAL SYMPTOMS MDR PULMONARY TB AT RSUD DR SOETOMO Ni Putu Anggita Medyantari; Soedarsono Soedarsono; Manik Retno Wahyunitisari
Jurnal Kedokteran Syiah Kuala Vol 19, No 2 (2019): Volume 19 Nomor 2 Agustus 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i2.18063

Abstract

Abstract. Multi-drug resistance is a condition where drugs rifampicin and isoniazid is not effective in killing bacteria Mycobacterium tuberculosis. Some of the causes of resistance to OAT are the use of a single drug in the treatment of tuberculosis, the use of drugs is inadequate and the irregular drug administration. In RSUD Dr. Soetomo, 25% of MDR Pulmonary TB patients do not want to be treated because symptoms are mild, no pain and feel the treatment is not comparable with the symptoms. This research is to determine the correlation between sex, age, nutritional status and diabetes mellitus with the clinical description of MDR pulmonary TB patients in RSUD Dr. Soetomo. Hence, it is expected  people can make the earliest possible prevention against this disease. In addition, clinicians are more aware of the symptoms of MDR Pulmonary TB. Method in this research is  descriptive observational study research. There were  103 patients taken as the sample of the study. Research variables consist of sex, age, nutritional status, and diabetes mellitus. The obtained data were analyzed by administering Chi- square analysis. The results of this study showed that each  variable tested in this study (sex, age, nutritional status and diabetes mellitus) associated with the symptoms of MDR Pulmonary TB  has  p 0.05 which means no significant relationship. There are no relation between sex, age, nutrition status and comorbidities with symptom of MDR pulmonary TB.  Keywords: multidrug resistance, sex, age, nutritional status, diabetes mellitus Multi-drug resistance adalah suatu kondisi dimana obat rifampisin dan isoniazid tidak efektif dalam membunuh bakteri Mycobacterium tuberculosis. Beberapa penyebab resistensi terhadap OAT adalah penggunaan obat tunggal dalam pengobatan tuberkulosis, penggunaan obat tidak memadai dan pemberian obat tidak teratur. Di RSUD Dr. Soetomo, 25% pasien TB paru MDR tidak mau diobati karena gejalanya ringan, tidak terasa sakit dan rasakan pengobatannya tidak sebanding dengan gejalanya. Penelitian ini bertujuan untuk mengetahui hubungan antara jenis kelamin, umur, status gizi dan diabetes mellitus dengan gambaran klinis pasien TB paru MDR di RSUD Dr. Soetomo. Sehingga diharapkan masyarakat bisa melakukan pencegahan paling dini terhadap penyakit ini. Selain itu, dokter lebih sadar akan gejala TB paru MDR. Metode yang digunakan dalam penelitian ini adalah penelitian observasional deskriptif. Terdapat 103 pasien yang diambil sebagai sampel penelitian. Variabel penelitian terdiri dari jenis kelamin, umur, status gizi, dan diabetes mellitus. Data yang diperoleh dianalisis dengan analisis Chi-square. Hasil penelitian ini menunjukkan bahwa setiap variabel yang diuji (jenis kelamin, usia, status gizi dan diabetes mellitus) yang dihubungkan dengan gejala TB paru MDR memiliki p 0,05 yang berarti tidak ada hubungan yang signifikan. Kesimpulannya, tidak ada hubungan antara jenis kelamin, usia, status gizi dan komorbiditas dengan gejala TB paru MDR.
Seorang Penderita Fistel Empiema Toraks Dengan Colostomy Bag Isa Ansori; Soedarsono Soedarsono
Jurnal Kedokteran Syiah Kuala Vol 6, No 1 (2006): Volume 6 Nomor 1 April 2006
Publisher : Universitas Syiah Kuala

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Abstract

Abstr  ak,  Laki,  26  tahun  MRS  dengan   keluhan  keluar  cairan  dari  lubang  bekas  pernasangan   toraks drain  sebelah   kanan  sejak  satu  tahun  yang  Ialu berwarna  coklai  kekuningan   keruh  terus  rnenerus,   foto toraks  PA/lateral    kanan   perselubungan   homogen  di hernitoraks  kanan  bawah,  BT A  positif  dua  (+ 2), biakan  I kultur  cairan  empierna   didapatkan    Acinetobacter   spp.,seramoeba    dengan   uji semi  kuantitatif hasil  positif.  Dilakukan   pasang  "window    fisrel"   atau  "open  drainage"   sebagai   "drainage"  cairan  pus yang   sudah    diencerkan    dengan   rnernasukkau    larutan   PZ  dan   diinstilasi    clengan    antibiotik    serta pemasangan   "colostomy bag" sebagai   penarnpung   cairan pus yang mengalir  keluar,   (.!KS 2006,·J:5-12) Kata   Kunci:    Empyerna  kronis, drainase  WSD. Abstract.  Male,  26 years old ran into a doctor  with  a complaint  that a kind  of fluid came  out  from the right  side  of the thoracic  drain hole.  The  fluid had  turbid yellowish  brown  that came  out continuously. This   condition    happened   for  about  one  year.  From  the  chest   radiograph    PA/on   the  right   lateral, hemithorax    homogeneous    veil  in  the  lower  right,   AFB   positive    (+ +),  culture   of  empyerna   fluid obtained  Acinetobacter  spp,  seramoeba   with  semi-quantitative    test  produced   a  positive    result.   The "window   fistel"  or "open  drainage"  as "drainage"  was perfomed  with  pus  that  diluted   by putting   into the PZ solution  and instilated    with antibiotics  and  the installation   of "colostomy   bag" as a container   of fluid  which  will  flowed out.(JKS 2006;1:5-12) Key wonts:  Chronic  Empyerna,  WSD
HUBUNGAN JENIS KELAMIN, USIA DAN PEKERJAAN DENGAN KEJADIAN ASFIKSIA GANTUNG DIRI DI RSUD DR SOETOMO TAHUN 2013-2016 Siti Ermawati; Bendrong Moediarso; Soedarsono Soedarsono
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 8 No 1 (2018): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2018.v08.i01.p04

Abstract

Latar Belakang: Bunuh diri merupakan fenomena yang terus meningkat dan hingga kini belum dapat dipahami secara pasti penyebab munculnya tindakan bunuh diri oleh seseorang. Bunuh diri merupakan penyebab kedua tertinggi kematian individu dengan rentang usia 15-29 tahun di dunia. Salah satu cara bunuh diri adalah mati gantung diri (hanging). Hanging adalah bentuk kematian akibat pencekikan dengan alat jerat, gaya yang bekerja pada leher berasal dari hambatan gravitasi berat tubuh atau bagian tubuh sehingga menimbulkan asfiksia lalu kematian. Tujuan: Mengetahui hubungan antara jenis kelamin, usia dan pekerjaan dengan kejadian asfiksia gantung diri di RSUD Dr Soetomo tahun 2013-2016. Metode: Merupakan jenis penelitian analitik desain cross sectional dengan sampel korban meninggal asfiksia di Instalasi Kedokteran Forensik & Medikolegal RSUD Dr Soetomo tahun 2013-2016. Hasil: dari 108 data meliputi 45 data korban meninggal karena asfiksia gantung diri dan 63 data korban meninggal karena asfiksia tidak gantung diri menunjukkan ada hubungan usia dengan kejadian asfiksia gantung diri (p=0,036) dan nilai contingency coefficient 0.221 yang artinya terdapat hubungan berkekuatan lemah. Namun, tidak ada hubungan antara jenis kelamin dengan terjadinya asfiksia gantung diri (p=1). Tidak ada hubungan antara pekerjaan dengan terjadinya asfiksia gantung diri (p=0,264). Kesimpulan: ada hubungan antara usia dengan kejadian asfiksia gantung diri. Namun, tidak ada hubungan antara jenis kelamin dan pekerjaan dengan terjadinya asfiksia gantung diri. Diperlukan sosialisasi kepada masyarakat tentang faktor risiko terjadinya asfiksia gantung diri agar kewaspadaan masyarakat meningkat dan asfiksia gantung diri dapat dicegah. Diperlukan penelitian lebih lanjut dengan variabel lain agar faktor risiko asfiksia gantung diri dapat diketahui secara pasti.
Co-Authors Abdul Rahman Bahmid Adnyana , I Made Dwi Mertha Agnes Dwi Sis Perwitasari, Agnes Dwi Sis Agung Dewi Sekar Agustinus Rizki Wirawan Riadi Akirasena, Mayoori Alvin Hartanto Kurniawan, Alvin Hartanto Amelia Lorensia ANAK AGUNG ISTRI AGUNG, SRILA NATASWARI Andri Dwi Wahyudi Andy Sulaiman Siregar Anindya Zalfaa Kusuma Dewi Anita Dewi Anggraini Anita Widyoningroem Bendrong Moediarso Budi Suprapti Budiono Budiono D. Sunarti Deby Kusumaningrum Diana, Adawiyah Putri Djohar Nuswantoro Dody Taruna Eko Budi Koendhori, Eko Budi Elisabeth Tri Wahyuni Widoretno Farah Nurul Rakhima H. Haryono Indri Ngesti Rahayu Irmawati M. Dikman Isa Ansori Jusak Nugraha Jusak Nugraha Kartono Wibowo Kuntaman Kuntaman Kusmiati, Tutik Laily Hidayati Lestari Dewi Lulus Handayani M. Yamin S. S Makhfudli Makhfudli ManikRetno Wahyunitisari Mohammad Yamin Sunaryo Suwandi Monica Dyah Puspitasari Muhammad Bagus Fidiandra Ni Made Mertaniasih Ni Putu Anggita Medyantari Nurul Wiqoyah, Nurul Pepy Dwi Endraswari, Pepy Dwi Permatasari, Ariani Pramanindyah Bekti Anjani Prasetya, Hanung Purwoko, Agus Putra, Oki Nugraha Putranto, J. Nugroho Eko Retno Budiarti Ricky Septafianty Rivan Virlando Suryadinata Ronald Pratama A. Ronald Pratama Adiwinoto Rosantia Sarassari Rudi Hariyono Safira Nur Ainiyah Setiawati, Rosy Sheila Gerhana Darmayanti Shelly Puspita Ayu Wardhani Siti Ermawati Stephanie Christina Sulaiman Tasalina Yohana Parameswari Gustam Titiek sulistyowati Tri Puji Astuti Umiastuti, Pirlina Wahyu Agung Purnomo Wahyu Setiani Wibowo Wahyu Setiani Wibowo Wayan Tunas Artama Yelvi Levani