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All Journal Majalah Kedokteran Bandung Jurnal Kesehatan Reproduksi Folia Medica Indonesiana Jurnal NERS UNEJ e-Proceeding Dentino: Jurnal Kedokteran Gigi Majalah Obstetri dan Ginekologi Dental Journal (Majalah Kedokteran Gigi) Jurnal Kesehatan Reproduksi Biomolecular and Health Science Journal Journal of Vocational Nursing
Suhartono Taat Putra
Departemen Patologi Anatomi Fakultas Kedokteran Universitas Airlangga
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO Carabelly, Amy Nindia; Taat Putra, Suhartono; Suardita, Ketut
Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

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Abstract

ABSTRACTThe latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 μg/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity
Psikoedukasi Dzikr Menurunkan Kadar Kortisol dan Meningkatkan Kadar IGG pada Ibu Primipara Wahyuni, Sri; Anies, Anies; Soejoenoes, Ariawan; Putra, Suhartono Taat
JURNAL KESEHATAN REPRODUKSI Vol 5, No 2 (2018)
Publisher : Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.398 KB) | DOI: 10.22146/jkr.37643

Abstract

Background: Unstable emotions that are common during the perinatal period affect hormonal regulation and affect immunity. Research of psychoeducation dhikr be important was done to reduce perceived stress so that cortisol levels can be controlled hence IgG increases.Purposes: to prove additional psychoeducation of dhikr in routine midwifery care more influential on decreasing cortisol and increasing IgG among primiparous women.Methods: This study was an experimental study. A number of 24 participants as intervention group and a number of 23 participants as control group. Cortisol and IgG levels measured using ELIZA kits in the third trimester of pregnancy, the three days and tenth days after birth. Statistical test using General Linear Model and independent t test to compare Δ score.Results: The result showed mean difference between groups on the end of interventions, cortisol 18.95, CI 95% (-13.42 – 51.33) and p value is 0.245. The difference of the IgG between groups 482.72, CI 95% (55,51 - 909,93) and p value is 0.028.Conclusions: Additional psychoeducation of dhikr in routine midwifery care has more decrease Cortisol and increase IgG levels in primiparous women. Keywords: Cortisol, Psychoeducation Dzikr, IgG, Primiparous
Kloning Gen Melanoma Antigen 1 (Mage-1) dari Jaringan Testis untuk mendapatkan Plasmid Rekombinan Mage-1 Mastutik, Gondo; I’tishom, Reny; Hardjowijoto, Sunaryo; Putra, Suhartono Taat
Majalah Kedokteran Bandung Vol 47, No 4 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Gen Melanoma antigen-1 (Mage-1) diekspresikan oleh sel spermatogonia jaringan testis normal dan diekspresikan 60−80% oleh liver penderita karsinoma hepatoseluler (KH). Ekspresi Mage-1 merupakan penanda untuk diagnosis KH serta prediktor kanker lambung dan kolorektal. Isolasi messenger ribonucleid acid (mRNA) Mage-1 dari jaringan liver penderita KH sulit dilakukan sehingga dilakukan isolasi mRNA Mage-1 dari jaringan yang mengekspresikan Mage-1, yaitu jaringan testis normal. Penelitian ini merupakan penelitian eksploratif yang dilakukan di Lembaga Penyakit Tropis Universitas Airlangga, Agustus 2006–Agustus 2008. Tujuan untuk mengkloning seluruh area koding gen Mage-1 dari jaringan testis pada vektor dan mendapatkan plamid rekombinan Mage-1. Isolasi seluruh area koding gen Mage-1 dilakukan dengan teknik semi-nested polymerase chain reaction (PCR). Seluruh area koding gen Mage-1 diisolasi, kemudian dikloningkan ke plasmid pET101/D-TOPO dan ditransformasikan ke Escherichia coli (E. coli) Top10 untuk mendapatkan plasmid rekombinan Mage-1. Panjang pET101/D-TOPO adalah 5.753pb dan area koding gen penyandi Mage-1 927 bp sehingga total panjang plasmid rekombinan 6.680 bp (5.753+927). Hasil analisis restriksi dengan EcoRV menunjukkan pita 4.230 dan 2.450 (4.230+2.450 = 6.680). Analisis sekuens gen Mage-1 dari testis mempunyai homologi 100% dengan sekuens M77481 serta NM_004988, dan 99% dengan BC01755. Simpulan, berdasarkan hasil analisis restriksi dan sekuens maka diperoleh plasmid rekombinan pETGM/MAGE1-Testis yang mengandung seluruh area koding gen Mage-1 dan dapat digunakan untuk pengembangan kit diagnostik karsinoma.  [MKB. 2015;47(4):199–206]Kata kunci:  Jaringan testis, karsinoma hepatoseluler, kloning, melanoma antigen-1, pET101/D-TOPOCloning of Melanoma Antigen 1 (Mage-1) Gene from Testicular Tissue to Obtain the Recombinant Plasmid Mage-1Melanoma antigen-1 (Mage-1) is expressed by spermatogonia cells of normal testicular tissue and 60−80% is expressed by the liver of hepatocellular carcinoma (HC) patients. Mage-1 expression is a marker for diagnosing HC and predicting gastric and colorectal cancers. Isolation of messenger ribonucleid acid (mRNA) Mage-1 from the liver tissue of HC patients is difficult; therefore, Mage-1 mRNA isolates can be obtained from tissues that express Mage-1 such as normal testicular tissues . This is an explorative research that was conducted at the Institute of Tropical Diseases of Airlangga University during August 2006–August 2008. The aim was to clone the coding sequence of Mage-1 gene from testicular tissues into a vector and to get recombinant plasmid Mage-1. Isolation of the full-length Mage-1 was performed using semi-nested polymerase chain reaction (PCR) which was then cloned into plasmid pET101/D-TOPO and transformed into Escherichia coli (E. coli) Top10 to get recombinant plasmid Mage-1. The length of pET101/D-TOPO was 5,753 bp and Mage-1 was 927 bp. The length of recombinant plasmid was 6,680 bp (5,753+927). Restriction analysis using EcoRV showed 4,230 and 2,450 bp bands (4,230+2,450=6,680). Sequence analyses showed that Mage-1 was 100% homologous with M77481 and NM_004988, 99% homologous with BC01755. In conclusion, according to the results of the restriction and sequences analysis, the recombinant plasmid pETGM/MAGE1-Testis contains the full length coding region of Mage-1 and is useful for developing the hepatocellular carcinoma diagnostic kits. [MKB. 2015;47(4):199–206] DOI: 10.15395/mkb.v47n4.621
[RETRACTED] IMPACT PSYCHOLOGICAL AND PSYCHO-PHYSICAL WORK DISTRESS ON TOOTH MOBILITY IN RAT MODEL (ARTICLE RETRACTED FROM IBSC PROCEEDING) Zahreni Hamzah; Suhartono Taat Putra; Elyana Asnar STP
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Retracted under author request.
THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO Amy Nindia Carabelly; Suhartono Taat Putra; Ketut Suardita
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2595

Abstract

ABSTRACTThe latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 μg/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity
Human pappilomavirus genotype in cervical tissue of patients with Cervical Intraepithelial Neoplasia (CIN) 1, CIN 2, and CIN 3 Gondo Mastutik; Rahmi Alia; Alphania Rahniayu; Anny Setijo Rahaju; Renny I’tishom; Suhartono Taat Putra
Majalah Obstetri dan Ginekologi Vol. 24 No. 3 (2016): September - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.113 KB) | DOI: 10.20473/mog.V24I32016.74-78

Abstract

Objectives: to determine the genotype of HPV in patients with precancerous lesions of cervical tissue.Materials and Methods: An observational study with cross sectional study of patients paraffin block CIN1, CIN2, CIN3 was conducted in Dr Soetomo Hospital. HPV DNA was extracted from paraffin blocks, then performed PCR and genotyping of HPV. The sample consisted of 28 patients with cervical tissue paraffin blocks CIN1, CIN2 and CIN3. Patients aged between 26-74 years (standard deviation 10,12).Results: HPV genotypes that infect patients with CIN1 were HPV16 and 18, CIN2 were HPV16 and 52 and CIN3 were HPV16, 67, and combined infection HPV16/67 and HPV52/67. HPV genotypes in a single infection were 26/28 (HPV16, HPV18, HPV52 and HPV67), and multiple infections were 2/28 (HPV16/67 and HPV52/67).Conclusion: The most dominant HPV genotypes infect patients with precancerous lesions of the cervix were HPV16, HPV67, HPV52, and HPV18.
Prevotella intermedia and Porphyromonas gingivalis in dental caries with periapical granuloma Risya Cilmiaty; Afiono Agung Prasetyo; Khilyat Ulin Nur Zaini; Mandojo Rukmo; Suhartono Taat Putra; Widya Asmara
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 4 (2013): December 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.71 KB) | DOI: 10.20473/j.djmkg.v46.i4.p213-217

Abstract

Background: Dental caries with necrotic pulp is a multifactorial disease that attacks enamel involving tooth pulp. The anaerobic bacteria infection in the pulp chamber could induce the formation of periapical granuloma. However, the presence of the most frequently anaerobic bacteria identified in apical periodontitis, Porphyromonas gingivalis and Prevotella intermedia, in periapical granuloma have not been confirmed. Purpose: The aims of study were to determine the presence of Porphyromonas gingivalis and Prevotella intermedia in dental caries with necrotic pulp and to determine its relation to periapical granuloma. Methods: Thirty-six patients of dental caries with necrotic pulp in Dr. Moewardi General Hospital in Surakarta, Indonesia were involved and classified into two groups, the group of patients with periapical granuloma and the group of patients without periapical granuloma. The caries tooth was extracted, and the chronic periapical tissue was swabbed and cultured on blood agar medium in anaerobic condition. The bacterial DNA was extracted from the positive cultures and subjected for Polymerase Chain Reaction (PCR). Results: Periapical granuloma was more likely found in women (OR 5.5, 95% CI=1.277-23.693; RR 2.5, 95% CI= 1.025-6.100). Black colonies bacteria were associated with periapical granuloma (OR 2.2, 95% CI=0.517-9.594; RR 1.5, 95% CI=0.655-3.623). Porphyromonas gingivalis and Prevotella intermedia were detected in group with or without periapical granuloma, however, only Prevotella intermedia was associated with periapical granuloma (OR 1.6, 95% CI=0.418-5.903; RR 1.3, 95% CI=0.653-2.393). Conclusion: The presence of Porphyromonas gingivalis and Prevotella intermedia in periapical granuloma were confirmed, however, only Prevotella intermedia were associated with periapical granuloma.Latar belakang: Karies gigi dengan pulpa nekrosis adalah penyakit multifaktorial yang menyerang enamel hingga ruang pulpa gigi. Infeksi bakteri anaerob di pulpa nekrosis dapat menginduksi pembentukan granuloma periapikal. Namun, keberadaan bakteri anaerob yang paling banyak ditemukan di periodontitis apikal, Porphyromonas gingivalis dan Prevotella intermedia, di granuloma periapikal masih perlu diteliti . Tujuan: Penelitian ini bertujuan meneliti keberadaan bakteri Porphyromonas gingivalis dan Prevotella intermedia, di karies gigi dengan pulpa nekrosis dan menganalisis kaitannya dengan granuloma periapikal. Metode: Tiga puluh enam pasien karies gigi dengan pulpa nekrosis di Rumah Sakit Umum Dr. Moewardi di Surakarta Indonesia dilibatkan dan diklasifikasikan dalam dua kelompok yaitu kelompok pasien dengan granuloma periapikal dan kelompok pasien tanpa granuloma periapikal. Gigi karies diekstraksi dan jaringan periapikal kronis diusap dan dikultur di media agar darah dalam kondisi anaerob. DNA bakteri diekstrak dari kultur yang positif dan dilakukan pemeriksaan dengan Polymerase Chain Reaction (PCR). Hasil: Granuloma periapikal lebih banyak ditemukan pada wanita (OR 5,5, 95% CI= 1,277-23,693; RR 2,5, 95% CI= 1,025-6,100). Koloni bakteri berwarna hitam diasosiasikan dengan granuloma periapikal (OR 2,2, 95% CI= 0,517-9,594; RR 1,5, 95% CI= 0,655-3,623). Porphyromonas gingivalis dan Prevotella intermedia terdeteksi di jaringan karies dental dengan atau tanpa granuloma periapikal, namun hanya Prevotella intermedia yang diasosiasikan dengan granuloma periapikal (OR 1,6, 95% CI= 0,418-5,903; RR 1,3, 95% CI= 0,653-2,393). Simpulan: Porphyromonas gingivalis dan Prevotella intermedia ditemukan di granuloma periapikal, namun hanya Prevotella intermedia yang terkait langsung dengan granuloma periapikal.
Dhikr (Recitation) and Relaxation Improve Stress Perception and Reduce Blood Cortisol Level in Type 2 Diabetes Mellitus Patients with OAD Faisal Amir; Gondo Mastutik; M Hasinuddin; Suhartono Taat Putra
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.024 KB) | DOI: 10.20473/fmi.v54i4.10707

Abstract

Recitation and relaxation are translated as stimuli that can turn disstraints into uneven conditions (balanced conditions). The purpose of this study was to explain the effect of remembrance and relaxation on improving stress perception and decreasing blood cortisol levels in patients with type 2 diabetes mellitus with Anti Diabetes Medication (OAD). This type of research is quasi-experimental with the non randomized control group pretest posttest design. The population in this study were all type 2 diabetes mellitus patients at the Internal Poly department of Syarifah Ambami Hospital Rato Ebu Bangkalan. The study sample was divided into 2 groups, namely control and intervention, each of which was 14 respondents. First, respondents were asked to fill out an informed consent form, identity, fill in the Depression Anciety Stress Scale 42 questionnaire (DASS 42) and examine cortisol levels as pre-treatment data. Then for 6 weeks the intervention group was given remembrance and relaxation treatment, while the control group was only given health education. After 6 weeks the second group respondents were asked to fill in DASS 42 and examined cortisol levels as data after treatment. Difference data before and after the treatment was tested for normality using Shapiro-Wilk and obtained normal data distribution so that the different tests used independent T test with a = 0.05. Recitation and relaxation can significantly improve stress perception with p value (0.001) <0.05 and reduce cortisol levels with p value (0.024) <0.05. Recitation and relaxation improve stress perception so that the hypothalamic pituitary adrenal (HPA) axis produces a balanced level of the hormone cortisol. Cortisol in a balanced level will improve various metabolic processes.
Increased Activity Of Mature Osteoblast from Rat Bone Marrow-Mesenchymal Stem Cells tn Osteogenic Medium Exposed to Melatonin Yugi Hari Chandra Purnama; Gondo Mastutik; Suhartono Taat Putra
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.119 KB) | DOI: 10.20473/fmi.v54i4.10714

Abstract

Exposure to melatonin in the cultures of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) in osteogenic medium is able to induce mesenchymal stem cells and preosteoblasts into active osteoblasts via several transduction signals such as ERK 1/2. Previous studies used a single dose of 50 nM and a physiological dose of 20-200 pg/ml. The objective of the study was to obtain an optimal dose of melatonin that enhances osteoblast activity by increasing the expression of ERK1/2 and ALP levels in the culture of Rat Bone Marrow Mesenchymal Stem Cells (BM-MSCs) in osteogenic medium. This study was an in vitro experimental laboratory study using BM-MSCs from rat femoral bone grown on osteogenic medium without or with exposure to melatonin in doses of 0, 50, 100, 150 nM for 21 days. BM-MSCs were characterized by immunocytochemical techniques (CD45- and CD 105+) and ERK 1/2 expression was checked 24 hours after exposure to melatonin, while ALP levels were examined on day 21 using ELISA technique. ERK 1/2 expression on BM-MScs exposed to melatonin in doses 0, 50, 100, and 150 nM were respectively 0.087, 0.095, 0.081, and 0.079. Mean ERK 1/2 expression in various groups showed a decrease along with increasing doses of melatonin. Among the four treatment groups, the administration of melatonin in a dose of 50 nM resulted in highest mean ERK 1/2 expression. ALP levels in BM-MSCs exposed to melatonin doses of 0, 50, 100, and 150 nM were 0.128; 0.130; 0.117, and 0.111 ng/ml respectively. Data showed that decreasing mean ALP levels occurred along with the addition of melatonin dose. In conclusion, the administration of melatonin 50 nM is the optimal dose to increase the differentiation of cultured rat BM-MSCs into active osteoblasts.
Self Management Program Meningkatkan Koping, Niat dan Kepatuhan Berobat Pasien PJK Hanim Mufarokhah; Suhartono Taat Putra; Yulis Setiya Dewi
Jurnal Ners Vol. 11 No. 1 (2016): April 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.189 KB) | DOI: 10.20473/jn.v11i1.1902

Abstract

Introduction: Management coronary artery disease required lifelong treatment. The successful management of CHD requires efective coping, intention and medication adherence of CHD’s patients. This study aimed to explain the changes of coping, intention, and medication adherence in patients with CHD after giving self management programme in RSUD Jombang based on Theory Planned Behavior.Methods: This study used a quasy experimental pretest-posttest control group design and 28 respondens selected by consecutive sampling. Independent variable was self management programme while dependent variables were coping, intention, medication adherence. Data were collected by using questonnaires measuring coping level, intention and medication adherence. The statistical test used was Mann Whitney, Wilcoxone Signed Rank and t-Test.Results: The result showed that 1) self management programme improve the level of coping in patients with CHD (p < 0,001), 2) self management programme improve the level of intention in patients with CHD (p < 0,001), 3) self management programme improve the level of medication adherence in patients with CHD (p < 0,001).Conclusion: To change a behavior requires improvement of coping and intentions. This is in according to the Theory of Planned Behavior that behavior change is influenced by the attitude toward the behavior, subjective norm, perceived behavioral control and intention.
Co-Authors Abu Bakar Afiono Agung Prasetyo Afria Arista Agus Sulistyono Ah Yusuf Ah. Yusuf Alphania Rahniayu Amy Nindia Carabelly Anies Anies Anies Anies Anny Setijo Rahaju, Anny Setijo Ariawan Soejoenoes Dwi Murtiastutik Elyana Asnar STP Faisal Amir Fanni Okviasanti Gondo Mastutik Hanim Mufarokhah I Gusti Ngurah Gunadi Sindhu Putra I'tishom, Reny Joko Suwito Joni Haryanto Ketut Suardita, Ketut Khilyat Ulin Nur Zaini Mandojo Rukmo Nabiha Missaoui Nurma Yuliyanasari, Nurma Olyvia Sita Aldisa Thadea Rahmi Alia Renny I’tishom Rifka Pahlevi Risya Cilmiaty Soejoenoes, Ariawan Sri Wahyuni Sri Wahyuni Sriyono Sriyono Sunaryo Hardjowijoto Trisniartami Setyaningrum Widya Asmara Yugi Hari Chandra Purnama Yulis Setiya Dewi Yusti Probowati Zahreni Hamzah