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All Journal AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrin : Jurnal Penelitian Pertanian JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Jurnal Proteksi Tanaman Tropis Jurnal Pengabdian Masyarakat Bangsa
Nur Prihatiningsih
Fakultas Pertanian Universitas Jenderal Soedirman, Purwokerto Jl. Dr. Suparno Karangwangal Purwokerto
Agriculture, Biological Sciences & Forestry Arts Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Applications of Potassium Fertilizer and Bacillus Sp. Biopesticide for Increasing Tomato Resistance to Bacterial Wilt Disease Prihatiningsih, Nur; Djatmiko, Heru Adi; Rochminarsi, Eny
AGRIVITA, Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bacterial wilt on tomato caused by Ralstonia solanacearum is a crucial disease, because it can reduce yield until 50%. The aims of this research were: 1) to find out biopesticide formula for Bacillus sp.growth, 2) to test Bacillus sp. against R. solanacearum in vitro, 3) to test potassium fertilizer combined with Bacillus sp. for enhancing tomato resistance to the bacterial wilt disease. The research was conducted in 2 steps i.e to test the persistence of Bacillus sp. In biopesticide formula, and to test the best combination of both potassium and the Bacillus sp. biopesticide. The results showed that Bacillus B298 was the best isolate in its persistence on the biopesticide formula of organic growth medium+CaCO3+CMC 1%+mannitol 1%, and in inhibiting R. solanacearum. The best biopesticide formula for the Bacillus sp. persistence was growth organic media+ CaCO3+CMC 1%+mannitol 1%. Bacillus sp. was able to increase tomato resistance to the bacterial wilt disease from the category of susceptible to be tolerant and becoming resistant.   Keywords: tomato, Ralstonia solanacearum, potassium and Bacillus sp.
SCREENING OF COMPETENT RICE ROOT ENDOPHYTIC BACTERIA TO PROMOTE RICE GROWTH AND BACTERIAL LEAF BLIGHT DISEASE CONTROL Prihatiningsih, Nur; Adi Djatmiko, Heru; Lestari, Puji
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.12078-84

Abstract

Screening of competent rice root endophytic bacteria to promote rice growth and bacterial leaf blight disease control. This study was aimed to collect isolate endophytic bacterial of rice roots which able to produce IAA, determine the effect of endophytic bacteria application in stimulating rice plant growth, and  evaluate the potential of rice root endophytic bacteria for controlling bacterial leaf blight. This reasearch was carried out at the Screen House, Plant Protection Laboratory, and Agrohorti Laboratory of the Agriculture Faculty, Jenderal Soedirman University. Isolation of rice root endophytic bacteria was carried out by purposive random sampling from several marginal lands. The results showed that 8 isolates of rice root endophytic bacteria were able to produce IAA, ranging from 57.56 to 79.33 ppm and B07 isolate from Serayu produced the highest amount of IAA. The B04 and B07 isolates were contributed to increase the rice plant growth. The application of rice root endophytic bacteria was effective in controlling bacterial leaf blight.
AKTIVITAS SIDEROFOR BACILLUS SUBTILIS SEBAGAI PEMACU PERTUMBUHAN DAN PENGENDALI PATOGEN TANAMAN TERUNG Prihatiningsih, Nur; Djatmiko, Heru Adi; Lestari, Puji
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.171 KB) | DOI: 10.23960/j.hptt.217170-178

Abstract

Siderophore activity of Bacillus subtilis as plant growth promoters and biological control agent of eggplants pathogens. The aims of this research were to identify the siderophores of B. subtilis, to assess its activities as plant growth promoters and biological control agent of eggplants pathogens. Five isolates of B. subtilis i.e.B46, B209, B211, B298 and B 315 grown on SDCASA medium. The isolate which showed the best siderophores production was then further studied on its ability as a growth promoter on eggplants in two soil types with different Fe content. The inhibitory test was conducted against two kinds of pathogens, namely Colletotrichum sp. and Ralstonia solanacearum. The greenhouse experiment was arranged using a factorial completely randomized block design. The first factor was the B. subtilis (B. subtilis B298 and without B. subtilis B298), second factor was the type of soil (Ultisol and Andisol). The variables measured were Fe uptake by plants, plant growth parameters on eggplant i.e. height, leaf number, root length, root volume, weight of fresh and dried shoot as well as fresh and dry root, percentage of inhibition to fungal and bacterial eggplant pathogens. The results showed that the five isolates of B. subtilis were able to produce siderophores as catecholate and hydroxamate types. The best siderophore production was showed by B. subtilis B298. The ability of B. subtilis B298 in accelerating the growth of plants was indicated by the increased of uptake Fe, plant height, leaf number, root volume, weight of dried plants by 45.62%, 25.48%, 19.45%, 41.10% and 34.89% respectively. The inhibition to the fungal and bacterial eggplant pathogens best shown by the isolates of B. subtilis B298 with 55.4% and 22 mm respectively.
CHARACTERIZATION OF EGGPLANT ENDOPHYTE BACTERIA AND RHIZOBACTERIA AS WELL AS THEIR ANTAGONISTIC ABILITY AGAINST Ralstonia solanacearum Saridewi, Larasati Puspita; Prihatiningsih, Nur; Djatmiko, Heru Adi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 2 (2020): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.220150-156

Abstract

Characterization of eggplant endophyte bacteria and rhizobacteria as well as their antagonistic ability against Ralstoniasolanacearum. Bacterial wilt caused by Ralsonia solanacearum is one of important diseases causing severe loses in eggplantproduction. Various strategies were used to manage bacterial wilt, including planting resistant varieties, soil amandement, andsoil solarization. However, management of R. solanacearum in eggplant by using endophytic bacteria and rhizobacteria werenot been done that much. The objective of this study was to: (1) characterization of endophytic and rhizobacteria; (2)determines the inhibition ability of endophytic and rhizobacteria isolates against R. solanacearum pathogen on eggplant. Thelaboratory experiment was arranged in completely randomized design with 5 treatments and 5 replications. The double layermethod using yeast peptone glucose agar (YPGA) medium was used in vitro test. Based on the morphological characteristicsthese isolates were suspected as a member of genus Bacillus. Among the isolates used in this study, TK isolate showed thebest capability to inhibit growth of R. solanacearum.
COGONGRASS ROOT EXTRACT FROM FIVE DIFFERENT SOILS TYPES FOR SUPPRESSING PURPLE BLOTCH AND INCREASING GROWTH AND YIELD OF SHALLOTS Rokhlani, Rokhlani; Soesanto, Loekas; Nur, Subandi; Prihatiningsih, Nur
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221103-115

Abstract

Cogongrass root extract from five different soils types for suppressing purple blotch and increasing growth and yield of shallots. The aim of this study was to examine the efficacy of cogongrass (Imperata cylindrica (L.) Beauv.) root extracts from five different soil types (Typic Udipsamments, Aeric Endoaqualfs (=Gleisal Eutrik), Typic Quartzipsamments (=Regosol Eutrik), Aquertic Chromic Hapludalfs, and Pachic Hapludolls) in suppressing purple blotch and increasing growth and yield of shallots. Split plot design was used with 13 treatments repeated three times, and 18 plants plot-1. The treatments consisted of control, fungicide propineb applied before and after inoculation, and five types of cogongrass root extract 50, 60, and 70% concentration applied before and after inoculation. Results showed that cogongrass root extract collected from Pachic Hapludolls which was applied before inoculation had significant effect on the highest pathosystem component indicated by delaying the incubation period, suppressing the intensity of the disease, slowing down the infection rate, and decreasing values of AUDPC as 41.85, 69.87, 75.13, and 67.63%, respectively, compared to control. The cogongrass root extract from Pachic Hapludolls applied before inoculation could increase plant fresh and dry weight plant-1, tuber weight plant-1, plant fresh and dry weight plot-1, and tuber dry weight plot-1 as 42.7, 49.6, 51.92, 66.75, 72.29, and 73.53%, respectively, compared to control.
MEKANISME ANTIBIOSIS BACILLUS SUBTILIS B315 UNTUK PENGENDALIAN PENYAKIT LAYU BAKTERI KENTANG Nur Prihatiningsih; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.855 KB) | DOI: 10.23960/j.hptt.11564-71

Abstract

Antibiosis mechanism of Bacillus subtilis B315 for controlling potato bacterial wilt disease. Bacillus subtilis B315 isolated from rhizospheric potato has antibiosis mechanism against Ralstonia solanacearum in vitro and become potentially used as controlling method of bacterial wilt in the field. The objectives of this research were to study the mechanism of B.subtilis B315 in controlling bacterial wilt disease, to study of B. subtilis B315 potency as both biocontrol and plant growth promoter, and to evaluate the mechanism as biocontrol agent. This green house experiment used CRD (Completely Randomized Design) with 5 treatments and 6 replicates. The treatments were control (without B. subtilis B315), B. subtilis B315 wild type, antibiosis mutant M16, antibiosis mutant M4, and antibiosis mutant M14. Variables observed were incubation period, disease index, infection rate, effectiveness of control, and growth components (i.e number of bud, plant height, leaf area, plant fresh and dry weight). The result of this research showed that B. subtilis B315 could delay incubation period, suppressed the disease index up to 64,9% and could promote the plant growth (leaf area). B. subtilis B315 had the antibiosis and other mechanisms that induced sistemic resistance. The implication of this research was that B. subtilis B315 could be used for biocontrol the bacterial wilt and promoted the potato growth.
ENZIM AMILASE SEBAGAI KOMPONEN ANTAGONIS BACILLUS SUBTILIS B315 TERHADAP RALSTONIA SOLANACEARUM KENTANG Nur Prihatiningsih; Heru Adi Djatmiko
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 16 No. 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.126 KB) | DOI: 10.23960/j.hptt.11610-16

Abstract

Enzyme amylase as an antagonist component of Bacillus subtilis B315 against potato Ralstonia solanacearum. One of the antagonist mechanism of Bacillus subtilis B315 is that it produced secundary metabolites. Enzyme amylase is produced by B. subtilis B315 as a secondary metabolite. The aims of the research were: (1) to test mechanism of antagonistics by B. subtilis B315 against potato Ralstonia solanacearum and (2) to detect antibiosis activity of B. subtilis B315. The research was conducted in the Laboratory of Plant Protection Agriculture Faculty and Laboratory of Integrated Research of Jenderal Soedirman University from April to October 2014. The research method is an experimental with growing double layer of B. subtilis B315 in YPGA medium and Ralstonia solanacearum in water agar. This research used a Completely Randomized Design with 3 treatments and 8 replications. The treatments were B. subtilis B1, B. subtilis B46 and B. subtilis B315. The activity of antibiosis was tested by amylase activity enzyme and than it was analyzed using FTIR (Fourier Transform-infra Red). Result of the research showed that B. subtilis B315 could suppress R. solanacearum growth with 14 mm inhibition zone. Antibiosis activity of B. subtilisB315 as biological agents was showed by the production of amylase enzyme by activity of 0,802 unit/ml. Analysis by FTIR was showed by the production of compound group of alkane, aldehyde, ketones, carboxylic acid, esther, amina, and amida.
Applications of Potassium Fertilizer and Bacillus Sp. Biopesticide for Increasing Tomato Resistance to Bacterial Wilt Disease Nur Prihatiningsih; Heru Adi Djatmiko; Eny Rochminarsi
AGRIVITA Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.33

Abstract

Bacterial wilt on tomato caused by Ralstonia solanacearum is a crucial disease, because it can reduce yield until 50%. The aims of this research were: 1) to find out biopesticide formula for Bacillus sp.growth, 2) to test Bacillus sp. against R. solanacearum in vitro, 3) to test potassium fertilizer combined with Bacillus sp. for enhancing tomato resistance to the bacterial wilt disease. The research was conducted in 2 steps i.e to test the persistence of Bacillus sp. In biopesticide formula, and to test the best combination of both potassium and the Bacillus sp. biopesticide. The results showed that Bacillus B298 was the best isolate in its persistence on the biopesticide formula of organic growth medium+CaCO3+CMC 1%+mannitol 1%, and in inhibiting R. solanacearum. The best biopesticide formula for the Bacillus sp. persistence was growth organic media+ CaCO3+CMC 1%+mannitol 1%. Bacillus sp. was able to increase tomato resistance to the bacterial wilt disease from the category of susceptible to be tolerant and becoming resistant.Keywords: tomato, Ralstonia solanacearum, potassium and Bacillus sp.
Pemberdayaan Kelompok Wanita Tani Melalui Optimalisasi Pemanfaatan Pekarangan dengan Budidaya Sayuran Organik Dataran Rendah Berbasis Kearifan Lokal dan Berkelanjutan Endang Warih Minarni; Darini Sri Utami; Nur Prihatiningsih
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 1 NOMOR 2 SEPTEMBER 2017 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.802 KB) | DOI: 10.30595/jppm.v1i2.1949

Abstract

The specific purpose of this activity is to increase the motivation of women in improving the quality of life and self-reliance in the family by optimizing the yard with organic vegetable farming in the lowlands based on Local Wisdom and Sustainable.  The strategy used was  the empowerment of target groups with the approach of Participatory Rural Appraisal, methods of education, training, demonstration plots, assistance and equipped with learning by doing techniques. The materials presented were: (1) garden  intensification technology with verticultur method, (2) organic vegetable cultivation technology based on liquid organic fertilizer, botanical pesticide and PGPR, (3) composting technology with enrichment of Trichoderma harzianum (Tricho-compos) activator of local microorganism, (4) technology of pest and disease control on environmentally friendly vegetable crops using botanical insecticides and microbial antagonists; (5) water use efficiency technology with drip irrigation; (6) good sorting, grading and packing technology, 7) empowerment technology to form groups and institutional strengthening and group management. The results of this activity are: (1) the target group / members The women farmer group has a very high curiosity about new information that can improve their standard of living,(2) technology transfer activities on the use of garden with organic vegetable cultivation received positive response from the members of the Group, (3) This devotional activity can encourage the pattern of mind and pattern of member action in organic vegetable cultivation by developing rural resources and sustainable local wisdom, (4) Verikultur cultivation technique with vertical paralon type on a land area of 10 m2 is economically profitable 
PENGARUH PEMASTEURAN TANAH TUNGGAL ATAU DIGABUNG AGENSIA HAYATI TERHADAP PENYAKIT BUSUK HATI DI PEMBIBITAN PISANG Joko Haryono; Nur Prihatiningsih; Rahman A. Wardhana; Loekas Soesanto
Agrin Vol 12, No 2 (2008): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2008.12.2.91

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh pemasteuran medium digabung dengan agensia hayatidan agensia hayati yang paling efektif terhadap penyakit busuk hati. Penelitian dilakukan di PT NusantaraTropical fruit, Lampung Timur dengan rancangan Petak Terbagi dan diulang tiga kali. Petak utama adalahmedium dipasteur atau tidak. Anak-petak adalah control dengan air steril, Trichoderma harzianum isolat jahedan pisang, Pseudomonas fluorescens P60, dan Fusarium equiseti diisolasi dari akar pisang. Peubah yangdiamati adalah masa inkubasi, intensitas penyakit, populasi konidium Fusarium akhir, akar berpotensi terinfeksi,tinggi tanaman, jumlah daun, jumlah akar primer dan sekunder, panjang akar, dan berat akar. Hasil penelitianmenunjukkan bahwa perlakuan gabungan memberikan hasil positif khususnya menurunkan intensitas penyakitsampai 43,57%. Agensia hayati T. harzianum isolat pisang dan P. fluroescens P60 yang digabung denganpemasteuran medium dapat menekan intensitas penyakit masing-masing sebesar 63,08 dan 59,75%. Agensiahayati yang paling efektif adalah T. harzianum isolat pisang karena menekan kepadatan Fusarium, meningkatkantinggi tanaman, dan meningkatkan berat akar masing-masing sebesar 41,12, 39,00, dan 98,86%.Kata kunci: busuk hati, bibit pisang, pemasteuran, agensia hayati ABSTRACTThe objectives of this research were to know the effect of pasteurized media combined with biologicalagents and the most effective biological agent on heart rot disease. The research was carried out at PT NusantaraTropical Fruit, East Lampung designed by Split Plot Design and repeated three times. The main plot waspasteurized and unpasteurized media. The subplot was control with sterile water or fungicide, Trichodermaharzianum isolated from ginger or banana, Pseudomonas fluorescens P60, and Fusarium equiseti isolated frombanana root. Variables observed were incubation period, disease intensity, late Fusarium conidial population,potentially infected root, crop height, leave numbers, primary or secondary root numbers, root length, and rootweight. Result of the research indicated that the combination treatments gave positively result specially to reducedisease intensity of 43.57%. Biological agents of T. harzianum banana isolate and P. fluorescens P60 combinedwith the pasteurisation could suppress disease intensity of 63.08 and 59.57%, respectively. The most effectivebiocontrol agent was T. harzianum banana isolate because of suppressing Fusarium density, increasing plantheight, and increasing root weight as 41.12, 39.00, and 98.86%, respectively.Key words: heart rot, banana seedlings, pasteurization, biological agents 
Co-Authors Adi Djatmiko, Heru Ali Djamhuri Bambang Hadisutrisno DARINI SRI UTAMI Dhadhang Wahyu Kurniawan Endang Warih Minarni Eny Rochminarsi Heru Adi Djatmiko Jaka Widada JAKA WIDADA Lafi Na’imatul Bayyinah Loekas Soesanto Loekas Soesanto Mutala’liah, Mutala’liah Nur Kholida Wulansari Nur, Subandi PUJI LESTARI Rahman A. Wardhana Rokhlani, Rokhlani Rostaman Saputra, Alil Saridewi, Larasati Puspita Siti Nurchasanah Triwidodo Arwiyanto Wiyantono, Wiyantono Wulansari, Nur Kholida