Izzata Barid
Bagian Ilmu Kedokteran Gigi Dasar, Fakultas Kedokteran Gigi, Universitas Jember, Indonesia

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SIFAT OSTEOINDUKTIF SILIKA AMORPHOUS SEKAM PADI : OSTEOINDUCTIVITY OF AMORPHOUS SILICA FROM RICE HUSK Didin Erma Indahyani; Zahreni Hamzah; Izzata Barid
Dentika: Dental Journal Vol. 16 No. 2 (2011): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.68 KB) | DOI: 10.32734/dentika.v16i2.1823

Abstract

Currently, bone graft is recommended to improve and support the biological repair of bone defects properly. Bone graft was found to cause death in the donor area, long pain, infection, cosmetic deformity, hematoma, the amount of blood loss, injury or damage to the nervous in the donor area. Synthetic bone graft material was developed as a scaffold that serves to template the formation of bone. The ideal scaffold should be biodegradable, osteoconductive and osteoinductive. During there graft materials have not optimally provide the morphological, mechanical properties, biocompatibility and biodegradation, and osteoinductive-osteoinductive. Silica is proven to support and promote primary osteoblast growth, synthesis of matrix proteins, collagen type I, so that it can cause bone formation. Rice husk contains of silica is high enough. Because of abundant material and its potential in bone formation, needs to be done research on silica from rice husk in the manufacture of synthetic bone graft. The purpose of this study was to analyze the amorphous silica from waste rice husks as synthetic bone graft material (scaffold), especially against osteoblasts proliferation. Type of study was an experimental laboratory. This research was conducted by isolating the amorphous silica from rice husk. Primary osteoblast cultures derived from calvaria rats aged 2 days, growing in Alpha Modified Eagle Medium (α-MEM) (as negative control), α-MEM that in condition with 58S silica (as positive control group) and α-MEM condition with silica from rice husk (as treatment group). Osteoblast proliferation was observed with Quick Cell Proliferation Assay Kit for 7 and 14 days. The result showed that the rice husk silica was significantly (p <0.05) osteoinductive, osteoblast cultures demonstrated that its proliferation was higher in the groups that were implanted in the culture medium condition with 58S silica. In conclusion, rice husk amorphous silica is potentially osteoinductive scaffold for synthetic bone graft material.
Analisis Peningkatan Remineralisasi Enamel Gigi setelah Direndam dalam Susu Kedelai Murni (Glycine max (L.) Merill) Menggunakan Scanning Electron Microscope (SEM) (The Analysis of Enamel Remineralization Increase in Pure Soy Milk (Glycine max (L.) Merill) Vievien - Widyaningtyas; Yani Corvianindya Rahayu; Izzata - Barid
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pure soy milk has a high mineral content, they are calcium and phosphor. Both minerals are important mineral in enamel remineralization process. Remineralization is the return process of mineral teeth that has detached to back return be hidroxyapatite cristal enamel. This research was aimed to analyze the enamel remineralization increase after being immersed in pure soy milk (Glycine max (L.) Merill). The type of this research is experimental laboratories with the post test control group design. The sampel of this research was first maxillary premolar which caries-free. The sample was divided into two groups (control and  pure soy milk treatment) and each group had four samples. The sample immersion was incubated in 14 days by incubator in 37°C, control group was immersed in artificial saliva and the treatment group was immersed in pure soy milk. The analysis of enamel remineralization used Scanning Electron Microscope with remineralization indicator was the depth of enamel microporocity. The result of this research showed that the enamel tooth samples that was immersed in pure soy milk had less microporocity depth than the sample that was immersed in artificial saliva, with significance level 0.000 (p<0.005). The conclusion of this research was pure soy milk can increase enamel remineralization. Keywords: calcium, enamel microporocity, phosphor, pure soy milk, remineralization, SEM
Analisis Kebocoran Tepi pada Glass Ionomer Kaca dengan Penambahan Bioactive Glass Berbasis Silica dari Ampas Tebu Catur Putri Kinasih; Didin Erma Indahyani; Izzata Barid; Niken Probosari
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 15 No 2 (2018)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v15i2.17931

Abstract

The use of Glass Ionomer Cement (GIC) has some limitation, such as water sensitivity which leads to formation of microleakage due to shrinkage and brittle. Incorporation of 0.04 wt% bioactive glass nano silica (BAG) with GIC enhances its bioactivity to forming hydroxycarbonate apatite (HCA) leading to preventing of marginal gap formation. The objective this study was To determine the difference of microleakage means value between GIC and GIC which has been added by BAG from bagasse. In this study making BAG from bagasse, than choose the samples (24 bovine) randomly and grouping them to be 4 groups, which are group 1, GIC; group 2, GIC+Vaseline, group 3, GIC+BAG; group 4 GIC+BAG+Vaseline. All of the samples are supposed to be preparation, placed of the restoration, and stored in the aquadest then methylen blue 1% in sequence at 37oC until 24 h. The microleakage means value is decided by scoring system depend on the penetration of methylen blue 1% at occlusal wall and gingival wall. The microleakage means value on GIC+BAG is smaller than GIC, but their differences were not significant.
Daya Hambat Ekstrak Daun Tembakau terhadap Pertumbuhan Mikroba Rongga Mulut Riria Hendarto Putri; Izzata Barid; Banun Kusumawardani
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 11 No 2 (2014)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Oral cavity diseases can be caused by the oral microbes  such as Streptococcus mutans, Porphyromonas gingivalis and Candida albicans. The oral microbes growth can be inhibited by chlorhexidine gluconate mouthwash, but its long term use have adverse effects, therefore it is important to do a further research to find effective antibacterial and antifungal as alternative substance mouthwash. Flavonoid compounds in tobacco leaf have potential as an antibacterial and antifungal. The objective was to analyze the inhibition effect of tobacco leaf extract to the growth of Streptococcus mutans, Porphyromonas Gingivalis and Candida albicans. This research used 20%, 40%, 60%, 80% and 100% ethanol extract of tobaco leaf,  1% DMSO as negative control and 0.2% chlorhexidine gluconate as positive control. Antibacterial and antifungal activities were tested by diffusion disc method with four repetitions. The observation was conducted by measuring the diameter of inhibition zone after incubating for 24 and 48 hours. The largest inhibition zone of Streptococcus mutans growth were producted by ethanol extract of tobacco leaf with 80% concentration, the largest inhibition zone on Porphyromonas gingivalis and Candida albicans growth were produced by ethanol extract of tobaco leaf with 100% concentration. Diameter of inhibition zone ethanol extract of tobacco leaf was equal with the diameter of inhibition zone of 0.2 % chlorhexidine gluconate. The conclusion described that ethanol extract of tobacco leaf can inhibit the growth of Streptococcus mutans, Porphyromonas gingivalis and Candida albicans.
Aktivitas Antioksidan dan Total Polisakarida Ekstrak Rumput Laut Merah, Hijau dan Coklat dari Pantai Jangkar Situbondo Didin Erma Indahyani; Depi Praharani; Izzata Barid; Ari Tri Wanodyo Handayani
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 16 No 2 (2019)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v16i2.23094

Abstract

The antioxidant activity and polysaccharide composition of seaweed is influenced by species (red, green and brown seaweed), geographical conditions, extraction methods, solvents, temperature and the length of time of extraction. The purpose of this study was to known the total polysaccharide and antioxidant activity in red, green and brown seaweed extracts from Jangkar Situbondo beach. Method. The study was conducted on red, green and brown seaweed extracted with ethanol and water to determine total polysaccharides. Antioxidant analysis uses the method 2,2-diphenyl1-picrylhydrazyl (DPPH) radical. Total polysaccharide and antioxidant activity were observed statistically using two-way ANOVA. The results of this study show that the polysaccharide content in seaweed water extract was significantly higher than ethanol extract, especially in brown seaweed. Seaweed water extracts showed higher antioxidant activity than ethanol extracts. The antioxidant activity of brown seaweed was significantly higher than that of green and red seaweed. Conclusion. Seaweeds have high antioxidant activity in water extracts. Brown seaweed water extract contains high polysaccharides than green and red seaweed. Antioxidant activity in brown seaweed is also higher than in green and red seaweed.
Viskositas dan Porositas Bahan Cetak Alginat dari Alga Merah Kappaphycus alvarezii Rosi Endah Pratiwi; Izzata Barid; Didin Erma Indahyani
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 19 No 2 (2022)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v19i2.34743

Abstract

Alginate impression material is a material that is often used in dentistry to produce study models. The main active ingredient in the alginate impression material is sodium alginate, which can be obtained naturally from algae. Alginate from turbulent water (Waters on village Agel, Situbondo) and the structure of aliginate gluronic acid can form gel and influence the viscosity and the porosity of alginate impression material. Purpose was to analyze the viscosity and porosity of the alginate impression material from the red algae Kappaphycus alvarezii. This study is a laboratory experimental study with the research design of the post-test only control group design. This study consisted of 4 groups, namely the (1) viscosity control group, (2) porosity control, (3) viscosity treatment, and (4) porosity treatment. The porosity measurement results showed that there was no significant difference and the viscosity was significantly different from the control group because of the type of algae, the surface area of the particles, the preparation stage, the extraction that affected sodium alginate. Kappaphycus alvarezii type red alginate impression material has lower porosity than the standard Hygedent alginate impression material with a mean yield of 9%. The viscosity of the Kappaphycus alvarezii red alginate impression material based on the sigma standard is classified as high viscosity with an average of 33,200 cP.
Analisis Ukuran Lengkung Rahang Anak Usia 10-13 Tahun dan 14-16 Tahun di Wilayah Dataran Tinggi (Studi di Kecamatan Jelbuk Kabupaten Jember) Izzata Barid; Didin E. Indahyani; Sigiq E. Utami
e-GiGi Vol. 11 No. 1 (2023): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v11i1.44391

Abstract

Abstract: The growth and development of the arch is influenced by genetics, race, and environment. Genetic factors are the important basic in growth and development while environmental factors in the form of geographical, socio-cultural and economic locations can interact with the genetic factors. This study aimed to analyze the size of the jaw arch of children aged 10-13 years and 14-16 years at the highland of Jelbuk District, Jember Regency. This was an analytical and observational study with a cross sectional design. Measurement of the jaw arch on the model was performed using the Raberin method. The results obtained 136 subjects consisting of 59 boys and 77 girls aged 10-16 years. All subjects were grouped into two age groups, 10-13 years and 14-16 years. The Mann-Whitney U test on the difference in jaw arch size between the ages of 10-13 years and 14-16 years showed that most of them had p-values of >0.05, which meant that there was no significant difference between the two age groups, except at L31 of mandibular male. In conclusion, there is no significant difference in the size of the jaw arch of children aged 10-13 years and 14-16 years at the highland of Jelbuk District, Jember Regency Keywords: highlands; the size of the jaw arch; growth spurts; socio-cultural; economy Abstrak: Pertumbuhan dan perkembangan lengkung rahang dipengaruhi oleh genetik, ras, dan lingkungan. Faktor genetik merupakan modal dasar dalam tumbuh kembang sedangkan faktor lingkungan berupa letak geografi, sosial budaya dan ekonomi dapat berinteraksi dengan faktor genetik. Penelitian ini bertujuan menganalisis ukuran lengkung rahang anak usia 10-13 tahun dan usia 14-16 tahun di wilayah dataran tinggi Kecamatan Jelbuk, Kabupaten Jember. Jenis penelitian ini observasional analitik dengan desain potong lintang. Pengukuran lengkung rahang pada model menggunakan metode Raberin. Hasil penelitian mendapatkan 136 subjek yang terdiri dari 59 anak laki-laki dan 77 anak perempuan berusia 10-16 tahun. Keseluruhan subjek dikelompokkan menjadi dua kelompok usia yakni usia 10-13 tahun dan usia 14-16 tahun. Berdasarkan Mann-Whitney U Test pada uji beda ukuran lengkung rahang antara usia 10-13 tahun dengan 14-16 tahun sebagian besar menunjukkan nilai p>0,05 yang artinya tidak terdapat perbedaan bermakna antara kedua kelompok usia, kecuali pada L31 rahang bawah anak laki-laki. Simpulan penelitian ini ialah tidak terdapat perbedaan bermakna dalam ukuran lengkung rahang antara kelompok usia 10-13 tahun dan 14-16 tahun di wilayah dataran tinggi Kecamatan Jelbuk, Kabupaten Jember. Kata kunci: dataran tinggi; ukuran lengkung rahang; growth spurt; sosial budaya; ekonomi
Aktivitas Antibakteri Bahan Cetak Berbasis Ekstrak Natrium Alginat dari Alga Merah (Kappaphycus alverezii) terhadap Lactobacillus acidophilus Putu Sadaka Putri Rahayu; Depi Praharani; Niken Probosari; Didin Erma Indahyani; Izzata Barid
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 1 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i1.38592

Abstract

Alginate impression material is a material that is often used for impression tissue in the oral cavity, and can be one of the agents of infection transmission in dental practice. Microorganisms can adhere to the mold such as Lactobacillus acidophilus bacteria. L. acidophilus were isolated from active caries and were considered as secondary invaders in the dental caries invasion process. The development of impression materials that have antibacterial capabilities needs to be done, one of which is by using materials derived from red algae (Kappaphycus alverezii). This plant contains a lot of alginate which is the main components of the cell wall, consisting of calcium, magnesium, sodium and potassium alginate salts. Synthesizing sodium alginate from red algae and analyzing the antibacterial activity of sodium alginate-based impression materials from red algae against L. acidophilus. Extraction of alginate from red algae using the acid method and conversion of alginic acid to sodium alginate by adding NaOH. The impression material is made with the addition of sodium alginate. Antibacterial activity test by well diffusion method. This study consisted of 3 groups, namely control (K), red algae sodium alginate extract (P1), and impression material with red algae sodium alginate extract (P2). Groups K, P1 and P2 had an average inhibition zone of 0 mm, 15.56 mm, 17.22 mm, respectively. Kruskal Wallis and Mann Whitney test showed significant differences between groups. Impression material based on sodium alginate extract from red algae has strong antibacterial activity against L. acidophilus.
Stabilitas Dimensi dan Setting Time Bahan Cetak Anatomis Kedokteran Gigi Dari Ekstrak Natrium Alginat Rumput Laut Merah (Kappaphycus alvarezii) Dari Desa Agel, Kecamatan Jangkar, Situbondo Didin Erma Indahyani; Izzata Barid; Agus Sumono; Fitria Arifka Rahman
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 1 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i1.38597

Abstract

Alginate impression material is important in dentistry. In its clinical application, setting time is still considered too fast, and the alginate impression material must have dimensional stability in accordance with ANSI / ADA standard specification no.18. Alginate impression material has a main component, namely sodium alginate which is produced from the seaweed extraction process. In Indonesia, there are several types of seaweed, one of which is the Kappaphycus alvarezii (K. alvarezii). The seaweed from the village of Agel Situbondo has the potential to be a source of sodium alginate and has antibacterial and antioxidant effects. The aim of this study to analyze the dimensional stability and setting time of the alginate printing material with sodium alginate extract of kappaphycus alvarezii seaweed. The study was conducted using laboratory experimental methods with post-test only control group design, there were 2 groups, namely the commercial alginate printing material control group, and the alginate printing material treatment group with sodium alginate extract from the acid method, then the FTIR test was carried out. Then the two groups were tested for setting time and dimensional stability. The results of the FTIR test of red seaweed extract showed an absorption peak that was identical to that of sodium alginate. The results of the setting time test significantly took longer for the red seaweed alginate impression material than the control group, while the dimensional stability showed the same results. Conclusion: The results of the setting time test showed that the treatment group had a longer setting time than the control group and both groups had dimensional stability according to the standard. The results of the setting time test showed that the treatment group had a longer setting time than the control group and both groups already had dimensional stability in accordance with the standard.
POTENSI BAHAN CETAK EKSTRAK NATRIUM ALGINAT RUMPUT LAUT MERAH (Kappaphycus alvarezii) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus mutans Inneke Dwi Lestari; Depi Praharani; Achmad Gunadi; Izzata Barid; Didin Erma Indahyani; Niken Probosari
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 10, No 1 (2023): Vol 10 No 1, Juni 2023
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v10i1.1120

Abstract

Introduction: Synthetic alginate is an impression material that is widely used in dentistry. Dental impressions may carry Streptococcus mutans bacteria, which is cariogenic bacteria. Red seaweed has the potential as a source of sodium alginate and has antibacterial activity. Aim: The purpose of this study is to analyze the ability of impression materials containing sodium alginate from red seaweed in inhibiting the growth of S. mutans bacteria. Methods: This study uses a laboratory experimental method with a post-test-only control group design. The number of research samples is 24, which are divided into group A (control), group B (red seaweed sodium alginate extract), and group C (red seaweed sodium alginate extract impression material). Sodium alginate was extracted from red seaweed (Kappaphycus alvarezii) by the acid method. Inhibition test of the growth of S. mutans bacteria using well diffusion method. Growth inhibition is characterized by the formation of an inhibition zone. Result: The test results showed that group A (control) does not have the ability to inhibit the growth of S. mutans bacteria. Meanwhile, group B (RLM Na- alginate extract) and group C (RLM Na-alginate extract impression material) have the same ability to inhibit the growth of S. mutans bacteria. Conclusions: Red seaweed sodium alginate extract impression material can inhibit the growth of S. mutans