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DETEKSI BAKTERI PATOGEN TERBAWA BENIH AKOR (ACACIA AURICULIFORMIS A. CUNN. EX BENTH.) Suharti, Tati; Joko, Tri; Arwiyanto, Triwidodo
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (811.395 KB) | DOI: 10.23960/j.hptt.11719-36

Abstract

Detection of seed-borne pathogenic bacteria of northern black wattle (Acacia auriculiformis A. Cunn. ex  enth.). Intensive research of seed-borne pathogen of A. mangium and A. crassicarpa which have been established in industrial timber estate (HTI) was undertaken in Indonesia, while plantings development of northern black wattle have recently been established in the 1990s. Very limited information available on northern black wattle diseases especially seed-borne diseases. The objectives of this study were to identify seed-borne pathogenic bacteria of northern black wattle and the effects on seed germination. Methods for the isolation of bacteria were by seed soaking, seed griding, blotter test, growing-on test on paper and soil. Identification of bacteria by PCR used 63F/1387R primer. The results showed that seed-borne bacteria of northern black wattle were Paenochrobactrum sp., Ralstonia sp., Burkholderia cepacia complex, Pseudomonas stutzeri, Acinetobacter sp., Alcaligenes faecalis, Salmonella bongori, Escherichia hermannii while pathogenic bacteria cause seedling leafspot were Micrococcus luteus and Burkholderia cepacia complex. Burkholderia cepacia complex, A. faecalis, Acinetobater sp., P. stutzeri, S. bongori and Ralstonia sp. reduced seed germination and increased rotten seed, suggested that they were the pathogenic bacteria of northern black wattle seed. Ralstonia sp. significantly increased the percentage of rotten seed and decreased shoot length and root length. P. stutzeri and S. bongori significantly inhibited the root growth. Paenochrobactrum sp. and E. hermannii were assumed as pathogen with weak virulence due to seed germination, the percentage of rotten seed and vigour index were relatively similar to untreated seed.
KARAKTERISTIK MORFOLOGI DAN FISIOLOGI BAKTERI ENDOFIT DAN RIZOBAKTERI DARI TANAMAN CENGKEH SEHAT dwimartina, fina; Joko, Tri; Arwiyanto, Triwidodo
Jurnal Agro Wiralodra Vol 4 No 1 (2021): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agro wiralodra.v4i1.58

Abstract

One of the determining factors in disease control is the use of superior seeds which have good quality and quantity. The use of biological control agents that form a symbiotic mutualism in their host plants can be used as an effort to obtain quality clove seeds. Endophytic bacteria and rhizobacteria have been reported as potential biological control agents for many plant diseases because of their safety in humans and non-target organisms. The purpose of this study was to identify the physiological and morphological characteristics of endophytic and rhizobacterial isolates obtained from clove plants. A total of 46 endophytic bacterial isolates were isolated from healthy clove roots, stems, leaves and flowers. Also, 66 isolates were isolated from the soil around the rhizosphere area. Based on the results of the identification of physiological and morphological characteristics, it is evident that the endophytic and rhizobacterial bacteria tested have the same character as Bacillus spp.
IMPLEMENTASI MANAJEMEN ORGANISASI SISWA INTRA SEKOLAH SEBAGAI STRATEGI DALAM PENGEMBANGAN KEPEMIMPINAN SISWA SMP NEGERI 2 SUKADANA Tri Joko
JURNAL LENTERA [PENDIDIKAN PUSAT PENELITIAN LPPM UM METRO] Vol 3, No 1 (2018): JUNE 2018
Publisher : Lembaga Penelitian UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.442 KB) | DOI: 10.24127/jlpp.v3i1.824

Abstract

ANALISIS RISIKO KESEHATAN LINGKUNGAN PAJANAN GAS HIDROGEN SULFIDA (H2S) PADA PEMULUNG AKIBAT TIMBULAN SAMPAH DI TPA JATIBARANG KOTA SEMARANG Bariyadi Rifai; Tri Joko; Yusniar Hanani Darundiati
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 3 (2016): MEI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.5 KB) | DOI: 10.14710/jkm.v4i3.13482

Abstract

Garbage decomposition process at landfill causes air pollution, one of which is hydrogen sulfide gas that is colorless ,highly toxic ,flammable and has a characteristic smell of rotten eggs. H2S gas can cause health problems,especially in the respiratory tract. The scavengers rarely wear masks while working in Jatibarang landfill. This study was done to analyze the environmental health risk assessment of H2Sgas to scavengers at Jatibarang landfill. This research is a cross-sectional research with Environmental Health Risk Analysis (ARKL) method. The sample in this study is male scavengers and settled in the landfill more than one year amounted to 65 people. The results of the measurement of H2S gas concentrations in Jatibarang landfill  with an average of 0.0057 ppm is below the threshold value. Projection exposure to real time, scavengers at the landfill Jatibarang do not have the risk of noncarcinogenic RQ (0.67 <1). Exposure projection for the next 5 years in there will cause a health risk noncarcinogenic (RQ> 1). In the calculation of individual scavengers found that 12.3% (8 people) scavengers already occurred non-carcinogenic risk (RQ> 1). Health complaints experienced by scavengers such as a headache, dizziness, irritation and pain in the respiratory tract.The conclusion of this study is the population of scavengers at the Jatibarang landfill doesnot have non-carcinogenic health risks. Scavengers will have a noncarcinogenic risk for about 10.43 years exposureduration (Dt) or in their next 3.43 years.
HUBUNGAN SANITASI LINGKUNGAN, PERSONAL HIGIENE DENGAN JUMLAH BAKTERI Escherichia coli PADA DAMIU DI KAWASAN UNIVERSITAS DIPONEGOROTEMBALANG Haryudi Okta Sofiyanto; Tri Joko; Nur Endah Wahyuningsih
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 4 (2016): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.97 KB) | DOI: 10.14710/jkm.v4i4.14441

Abstract

Air merupakan sumber daya alam yang sangat penting dalam kehidupan. Di Indonesia kebutuhan air sebanyak 200-400 liter per orang per hari. Berdasarkan data Dinas Kesehatan Kota Semarang terdapat 289 depot air minum isi ulang. Jumlah depot air minum yang ada dalam penelitian ini sebanyak 28 sampel di Kawasan Universitas Diponegoro, Tembalang, Kota Semarang, yang terdapat di Kelurahan Ngesrep, Tembalang dan Srondol. Tujuan dari penelitian ini menganalisis hubungan sanitasi lingkungan, personal higiene dengan jumlah  bakteri Escherichia coli pada depot air minum isi ulang di Kawasan Universitas Diponegoro Tembalang, Kota Semarang. Merupakan penelitian observasional dengan pendekatan cross sectional. Populasi dalam penelitian ini adalah depot air minum Isi ulang sebanyak 28 depot. Sampel yang diambil menggunakan populasi jenuh. Analisis data menggunakan uji Chi square dengan taraf signifikansi α = 5 %. Berdasarkan uji statistik diketahui tidak ada hubungan antara kondisi sanitasi lingkungan dengan jumlah bakteri Escherichia coli (p value = 1,000), tidak ada hubungan antara kondisi pengolahan depot air minum isi ulang dengan jumlah bakteri Escherichia coli (p value = 0,124), tidak ada hubungan antara kondisi kebersihan galon dengan jumlah bakteri Escherichia coli (p value = 1,000), tidak ada hubungan antara kondisi ruangan pengisian dengan jumlah bakteri Escherichia coli (p value = 0,724), tidak ada hubungan antara personal hygiene karyawan dengan jumlah bakteri Escherichia coli (p value = 0,955). Penelitian ini dapat disimpulkan tidak ada hubungan sanitasi lingkungan, personal higiene dengan Jumlah bakteri Escherichia coli pada Depot Air Minum Isi Ulang di Kawasan Universitas Diponegoro, Tembalang, Kota Semarang
KEMAMPUAN KOAGULAN KITOSAN DENGAN VARIASI DOSIS DALAM MENURUNKAN KANDUNGAN COD DAN KEKERUHAN PADA LIMBAH CAIR LAUNDRY (Studi pada Rahma Laundry, Kecamatan Tembalang, Kota Semarang) Dyah Agustin Catur Putri; Tri Joko; Niki Astorina Yunita Dewanti
Jurnal Kesehatan Masyarakat (Undip) Vol 3, No 3 (2015): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.591 KB) | DOI: 10.14710/jkm.v3i3.12637

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Laundry liquid waste contains several chemical substances in detergent raw materials such as phosphate, surfactants, ammonia, and total suspended solids. The existence of detergent in high concentrations and exceeds the quality standards that have been estabilished in a body of water can lead to cases of enviromental pollution in the form of increased turbidity an Chemical Oxygen Demands (COD) levels. Therefore in order to maintain and to ensure the availabillity of water in terms of quality, it requires coagulation-flocculation process to laundry liquid waste before discharging into water bodies. This study aims to determine the decrease of COD levels and turbidity level in laundry liquid waste using chitosan coagulant in Rahma Laundry, Tembalang District, Semarang. The research is a quasi experimental study with pretest-posttest with control group research design with 6 times replication. The total samples are 60 in wich 24 tested for the levels of turbidity and 6 controls. The test results of Kruskal-Wallis with significance p-value < 0,05 indicates that dosage variation (p=0,000) gives different levels of COD and dosage variation (p=0,000) provide 755,97 mg/l and the advantage levels of turbidity before treatment was 516,20 NTU. The optimum dosage of chitosan coagulant is on the dose of 200 mg/l with the effectiveness decrease of COD levels and turbidity levels on 72,67% an 98,67% respectively.
PERBEDAAN VARIASI PENAMBAHAN MEDIA ADSORPSI KONTAK AERASI SISTEM NAMPAN BERSUSUN (TRAY AERATOR) TERHADAP KADAR BESI (Fe) AIR SUMUR GALI DI DESA JATIHADI KECAMATAN SUMBER KABUPATEN REMBANG Savitri Rachmawati; Tri Joko; Nikie Astorina Yunita Dewanti
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 3 (2016): MEI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.097 KB) | DOI: 10.14710/jkm.v4i3.13696

Abstract

People in the Jatihadi village are using dug wells water which contain high iron level. The average of iron level is 2,79 mg/l. Appropriate technology tray aerator with media contacts zeolite and activated carbon is the right solution to overcome the problems of high Fe content. This study aims to analyze the difference variation the addition of contact media adsorption on tray aerator for dug wells iron level in Jatihadi village, Sumber subdistrict, Rembang regency. The design of this study is pre experiment research with one group pretest posttest design.  We used Inductively Coupled Plasma (ICP) method to measure the iron level. Water sample volume for each aeration is 20 liters and there are 16 repetitions. The media that we used in this research is zeolite and activated carbon. The result showed average levels of iron before treatment is 2,79 mg/l, and after the first treatment down to 0,21 mg/l, after second treatment is 0,25 mg/l. Statistical analysis showed that there is no difference between variation of contact media adsorption for dug wells  iron level, but Wilcoxon analysis showed that there is a significant difference between before and after treatment for adding zeolite or activated carbon. The efficiency of tray aerator with the addition of zeolite is higher than addition of activated carbon.
Hubungan Paparan Pestisida Dengan Kadar HB (Hemoglobin) pada Petani Penyemprot Pestisida di Desa Sumberejo Kecamatan Ngablak Magelang (PESTICIDE EXPOSURE RELATIONSHIP WITH HB (HEMOGLOBIN) CONTAINS TO FARMERS PESTICIDES IN SUMBEREJO DESA DISTRICT NGABLAK M Fianti andua; Onny Setiani; Hanan Lanang Dangiran; Tri Joko
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 3 (2017): MEI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.212 KB) | DOI: 10.14710/jkm.v5i3.17251

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Pesticide chemical are chemical compounds that are toxic, which is used to eradicate plant pests or weeds. The impact of pesticides can be on farmers sprayers, families and neighbors. The purpose of this study was to know the exposure of pesticides and hemoglobin (HB) to pesticide spraying farmers in the village of Sumberejo Ngablak District Magelang. Sampel as many as 43 farmers with cross sectional with purposive sampling method. The results showed the relation between the completeness of APD (p = 1,000), spraying frequency (p = 1,000), spraying time (p = 0,029), pesticide type (0,515), kholinesterase (p = 0,421) with Hb. This research has no relationship of exposure of pesticide with Hb level in Sumberejo village, Ngablak Magelang sub-district.
HUBUNGAN ANTARA PRAKTIK 3M DAN FAKTOR LINGKUNGAN FISIK RUMAH DENGAN KEJADIAN DEMAM BERDARAH DENGUE DI WILAYAH KERJA PUSKESMAS KAYEN KABUPATEN PATI Ulfah Rahmawati; Tri Joko; Nurjazuli Nurjazuli
Jurnal Kesehatan Masyarakat (Undip) Vol 6, No 6 (2018): NOVEMBER
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.716 KB) | DOI: 10.14710/jkm.v6i6.22209

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One of the diseases that include extraordinary events is Dengue Hemorrhagic Fever (DHF). The incidence of dengue hemorrhagic fever is strongly influenced by existing environmental conditions and eradication of mosquito breeds such as 3M practices. The purpose of this study is to analyze the relationship between physical environmental factors of the house and 3M practices at Puskesmas Kayen work area in the Pati district. The variables in this research are 3M practice (cover, drain and bury) and environmental factors (breeding place and resting place inside and outside the house). This research is an observational research with cross sectional study approach. The population in this study is the community of DHF patients recorded at puskesmas Kayen work area in the pati district and non-dengue fever with 60 respondents. Sampling using the latest data by using sampling technique is purposive sampling. The data in this research is obtained from direct interview and observation. Then, the results of data that have been collected and analyzed using chi square with 95% confidence level followed by calculate the risk / value of OR. The results of this study indicate a relationship between the resting place / resting place outside the home (p = 0.038, OR = 3,5), the practice of closing the water reservoir (p = 0.019, OR = 4,125) and the practice of draining the water reservoir (p = 0.004, and OR = 5.5). This study concludes the relationship of resting places outside the home, the practice of draining the water reservoir and the practice of closing the water reservoir with the incidence of dengue hemorrhagic fever in the work area Puskesmas kayen Pati District. Therefore, it needs to be intensified PSN 3M campaign to the community to prevent DHF incidence.
VARIASI KONSENTRASI EM4 DALAM PROSES PEMBUATAN KOMPOS LINDI Candra Rulyana; Nurjazuli Nurjazuli; Tri Joko
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 5 (2017): SEPTEMBER
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.36 KB) | DOI: 10.14710/jkm.v5i5.19175

Abstract

Large amounts of waste require special handling to reduce negative impacts. One way to reduce the negative impact of waste generation is waste processing by adding various EM4 as decomposers. The result of organic waste processing of household is leachate compost. The purpose of this research is to know the ability to use different variation of Effective Microorganism 4 (EM4) concentration in composting process. The type of research used is experimental semu (Quasi Experiment). With the design of Time series research with the Control Time Series Design. Total volume of household waste generated at the research location is 908 liters. The composition of waste generation is 378 liters of inorganic waste and 530 liters of organic waste. The percentage of household waste generated in this research is 58% organic waste and 42% of inorganic waste. In this study the average waste generated is 1.7 liters / person. The highest temperature is in the addition of EM4 with a concentration of 4% ie 34.5⁰C. The highest average daily temperature was found in the addition of EM4 2% concentration of 30⁰C. The highest compost volume of compost produced by composter with the addition of EM4 2% concentration as much as 5.9 liters. The average daily volume of the largest compost leachate produced composter with the addition of EM4 with a concentration of 2% ie 0.19 liters / day. Compost volume at least produced composter with the addition of EM4 with a concentration of 2% is 3.5 liters. The largest percentage of waste reduction to compost on the composter with the addition of EM4 with a 2% concentration of 88.3%. Suggestions for households should process organic waste caused into leach compost because it reduces the volume of waste that should be disposed in waste disposal.
Co-Authors - Suhartono Abdullah, Jilan Tsani Achmadi Priyatmojo Adi, Bayu Kusuma Aditya, Vanessa Rizky Agus Bintara Birawida Ahmad Zaerozi Ani Widiastuti, Ani Annisa Aulia Nurrohmah Anugrah Febrino Balwa Arif Wibowo Arlyna Budi Pustika Asma Afifah Astorina, Nikie Azmi Umi Anisyah Bariyadi Rifai Bella Arieza Andriyana Bibit Nasrokhatun Diniah Budi Waloyo Budiyono Budiyono Burhanuddin Thohir Candra Rulyana Charles foeh Foeh Choirul Luluk Fatimah Cornelius Saryadi Turnip Delima Kurnia Sari Devita Nur Aprilia Dwimartina, Fina Dyah Agustin Catur Putri Eka Luvita Sari Eko Handoyo Elisa Maharani Elzha Af’idatul Himmah Endang Sulistyaningsih Farida Sugiester S. Fianti andua Fitria, Ida Hanan Lanang Dangiran Hariyanti, Fika Harper, Stephen Haryudi Okta Sofiyanto Hidayanty, Healthy Ijaz ul haq Ilma, Hurin Nabila Aghnia Intan Aulia Putri Irawati, Roosdiana Jalaluddin Abdul Malek, Jalaluddin Abdul Jayawarsa, A.A. Ketut Karroghi, Silmi Aziza Kurniasih, Budiastuti Lisa Okta Permatasari Maulida Khairunnisa McCann, Honour C. Muhammad Addin Rizaldi Mursid Rahardjo Mursid Raharjo Mustafiroh Kasanah Nabiha, Puteri Inandin Nabilah Nurhidayanti Nasrokhatun Diniah, Bibit Ngazis, Muhammad Niki Astorina Yunita Dewanti Nikie Astorina Yunita Dewanti Nila Himayati Nilna Fasyya Salsabila Noor Zahrotul Muniroh Nur Endah Wahyuningsih Nur Fajria Safarina, Ismi Nurdiyanto, Miftakhul Nurjazuli Nurjazuli Nurjazuli Nurjazuli Nurjazuli Onny Setiani Pratiwi, Annisa Hasta Purnomo Purnomo Putri, Novi Hervianti Qorina Sabila Fa&#039;iza Raharjo , Mursid Rahmi Nur Alifiani Restu Andri Setiyanto Riska Triafryani Putri Rozi, Choirul Ruba, Yohanes Emanuel S. Russeng, Syamsiar Sahiledengle, Biniyam Salahuddin, Nurul Syahriani Santya Nareswari Saraswati, Vivian Thea Savitri Rachmawati Sevirasari, Nindy Shofa Rakhmatika Shrestha, Akina Sinta Nugraheni Dewi Siti Nurkhayati Siti Subandiyah Slamet Wardoyo Sukri Palutturi SULISTIYANI SULISTIYANI Sundari, Dini Suriah Suriah Suryanti Tati Suharti Triwidodo Arwiyanto Ulfah Rahmawati Vinidia Pertiwi Wahiduddin Wahiduddin Wakhid Anwar Wardana, Lalu Muhammad Fikri Wibowo, Eko Prasetyo Wisanggeni, Gen Adi Woldesenbet, Biruk Yuni Pradilla Fitri, Yuni Pradilla Yusniar Hanani Danudianti Yusniar Hanani Darundiati