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EFEK ANTIANGIOGENIK EKSTRAK METANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) PADA MEMBRAN KORIO ALANTOIS (CAM) EMBRIO AYAM YANG TERINDUKSI bFGF Salamah, Nina; Sugiyanto, Sugiyanto; Hartati, Mae Sri; Hayati, Farida
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.799 KB) | DOI: 10.14499/mot-TradMedJ15iss1pp%p

Abstract

Angiogenesis merupakan peristiwa pertumbuhan pembuluh darah baru, yang memungkinkan sel kanker mendapatkan suplai nutrisi dan oksigen, sehingga dapat terus bertahan hidup. Pasak bumi (Eurycoma longifolia, Jack) merupakan salah satu tanaman asli Indonesia yang memiliki potensi anti kanker. Ekstrak metanol, butanol, kloroform, dan air dari akar pasak bumi terbukti memiliki efek sitotoksik terhadap beberapa sel kanker. Penelitian ini bertujuan untuk mengetahui efek antiangiogenik ekstrak metanol akar pasak bumi pada CAM embrio ayam. Uji penghambatan angiogenesis dilakukan dengan membagi telur berembrio umur 8-9 hari dalam 8 kelompok perlakuan. Kelompok I sebagai kontrol paper disc, kelompok II sebagai kontrol bFGF, kelompok III sebagai kontrol bFGF+ pelarut DMSO 0,8%, kelompok IV, V, VI, VII dan VIII sebagai kelompok uji penghambatan angiogenesis, pada paper disc diberi bFGF 1 ng/μL dan ekstrak metanol akar pasak bumi berturut-turut dengan dosis 20, 40, 60, 80 dan 100 μg/mL. Setelah diinkubasi selama 3 hari (umur embrio12 hari), telur dibuka dan isi telur dikeluarkan, kemudian CAM yang melekat pada cangkang diamati secara makroskopik dan mikroskopik. Ekstrak metanol akar pasak bumi memberikan aktivitas antiangiogenik mulai kadar 40 μg/mL. Peningkatan konsentrasi ekstrak metanol akar pasak bumi meningkatkan aktivitas penghambatan angiogenesis.
Farmakokinetika Rifampisin akibat Praperlakuan Jus Kulit Buah Manggis pada Tikus Wistar Jantan Pradana, Dimas Adhi; Hayati, Farida; Praptiwi, .
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 2 (2014)
Publisher : Indonesian Research Gateway

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Abstract

Aim of this study was to determine the effect of pre-treatment of mangosteen rind juice on the pharmacokinetics of rifampicin on white male Wistar rats. Rats (10) devided into 2 groups, each group consisted of 5 rats. Group I rats were given a dose of rifampicin 50 mg/kg body weight as the control group. Group II rats were given pre-treatment  mangosteen rind juice 200 mg/kg for 7 days and then on day 8 were given rifampicin 50 mg/kg and ma ngosteen rind juice 200 mg/kg as the treatment group. On the hour of 0,25; 0,5; 1; 1,5; 2; 3; 4; 6; 8; 10; 12 and 24  blood sample was taken and then deter-mined the rifampicin plasma levels by HPLC. The results obtained produced curve rifampicin levels in the blood versus time and pharmacokinetic parame-ters of rifampicin such AUC0-∞ , AUMC0-∞, and MRT and MRT can be determined.The results showed that pre-treatment mangosteen rind juice did not have a signiϔicant effect on the pharmacokine tics of rifampicin based parameters AUC0-∞, AUMC0-∞, and MRT.Keywords: pharmacokinetics, mangosteen rind, rifampicin Penelitian ini bertujuan untuk mengetahui pengaruh praper-lakuan jus kulit manggis terhadap proϐil farmakokinetika rifampisin pada ti-kus Wistar jantan. Hewan uji terdiri dari 2 kelompok, tiap kelompok terdiri dari 5 ekor tikus. Kelompok I, tikus diberi rifampisin dengan dosis 50 mg/kg BB sebagai kelompok kontrol. Kelompok II tikus diberi praperlakuan jus kulit manggis 200 mg/kg BB selama 7 hari kemudian pada hari ke-8 diberi rifampisin 50 mg/kg BB sebagai kelompok perlakuan bersama jus kulit mang-gis 200 mg/kg BB. Pada jam ke 0,25; 0,5; 1; 1,5; 2; 3; 4; 6; 8; 10; 12; dan 24 di-ambil sampel darah kemudian ditetapkan kadar rifampisin dalam sampel da-rah dengan HPLC. Hasil yang diperoleh dibuat kurva kadar rifampisin dalam plasma terhadap waktu kemudian ditentukan nilai parameter farmakokinetik rifampisin seperti AUC0-∞, AUMC0-∞, dan MRT. Hasil penelitian menunjukkan bahwa pemberian praperlakuan jus kulit manggis tidak memberikan penga-ruh yang signiϐikan terhadap farmakokinetika rifampisin berdasarkan para-meter AUC0-∞, AUMC0-∞, dan MRT.Kata kunci: farmakokinetika, kulit manggis, rifampisin
Farmakokinetika Rifampisin akibat Praperlakuan Jus Kulit Buah Manggis pada Tikus Wistar Jantan Pradana, Dimas Adhi; Hayati, Farida; Praptiwi, .
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v7i2.162

Abstract

Aim of this study was to determine the effect of pre-treatment of mangosteen rind juice on the pharmacokinetics of rifampicin on white male Wistar rats. Rats (10) devided into 2 groups, each group consisted of 5 rats. Group I rats were given a dose of rifampicin 50 mg/kg body weight as the control group. Group II rats were given pre-treatment  mangosteen rind juice 200 mg/kg for 7 days and then on day 8 were given rifampicin 50 mg/kg and ma ngosteen rind juice 200 mg/kg as the treatment group. On the hour of 0,25; 0,5; 1; 1,5; 2; 3; 4; 6; 8; 10; 12 and 24  blood sample was taken and then deter-mined the rifampicin plasma levels by HPLC. The results obtained produced curve rifampicin levels in the blood versus time and pharmacokinetic parame-ters of rifampicin such AUC0-â?? , AUMC0-â??, and MRT and MRT can be determined.The results showed that pre-treatment mangosteen rind juice did not have a signiÏ?icant effect on the pharmacokine tics of rifampicin based parameters AUC0-â??, AUMC0-â??, and MRT.Keywords: pharmacokinetics, mangosteen rind, rifampicin Penelitian ini bertujuan untuk mengetahui pengaruh praper-lakuan jus kulit manggis terhadap proϐil farmakokinetika rifampisin pada ti-kus Wistar jantan. Hewan uji terdiri dari 2 kelompok, tiap kelompok terdiri dari 5 ekor tikus. Kelompok I, tikus diberi rifampisin dengan dosis 50 mg/kg BB sebagai kelompok kontrol. Kelompok II tikus diberi praperlakuan jus kulit manggis 200 mg/kg BB selama 7 hari kemudian pada hari ke-8 diberi rifampisin 50 mg/kg BB sebagai kelompok perlakuan bersama jus kulit mang-gis 200 mg/kg BB. Pada jam ke 0,25; 0,5; 1; 1,5; 2; 3; 4; 6; 8; 10; 12; dan 24 di-ambil sampel darah kemudian ditetapkan kadar rifampisin dalam sampel da-rah dengan HPLC. Hasil yang diperoleh dibuat kurva kadar rifampisin dalam plasma terhadap waktu kemudian ditentukan nilai parameter farmakokinetik rifampisin seperti AUC0-â??, AUMC0-â??, dan MRT. Hasil penelitian menunjukkan bahwa pemberian praperlakuan jus kulit manggis tidak memberikan penga-ruh yang signiϐikan terhadap farmakokinetika rifampisin berdasarkan para-meter AUC0-â??, AUMC0-â??, dan MRT.Kata kunci: farmakokinetika, kulit manggis, rifampisin
The effect of extract Kaempferia galanga rhizomes on the elimination kinetics of quinidine in rabbits Hayati, Farida; Hakim, Lukman
Indonesian Journal of Pharmacy Vol 14 No 4, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.269 KB) | DOI: 10.14499/indonesianjpharm0iss0pp177-181

Abstract

The research was aimed to observe the effect of the juice of Kaempferia galanga rhizomes on quinidine elimination kinetics in rabbits. The study on interaction of extract Kaempferia galanga rhizomes to quinidine elimination kinetic was conductedly employing a cross randomized design using male rabbits which were divided into 3 groups (6 rabbits for each group). The groups were given a single oral quinidine 30 mg/kg BW as a control group and were conferred single oral extract “kencur” 4.8 mi/kg BW each dose for 4 days prior to the treatment with quinidine. Serial blood samples(0,2 ml) were withdrawn at various interval time via the ear marginal for spectrofluorometric analysis of unchanged quinidine in blood. The concentration of quinidine was determined using a standard curve and the concentration to time data was used to determine quinidine elimination kinetics i.e. Clt and MRT. The results indicated that extract “Kencur” was found to decrease quinidine clearance 17,19% and 7,07% (P>0,05) and increase quinidine MRT198,89% and 71,70% (p>0,05).Key words : Kaempferia galanga rhizomes, quinidine, elimination
KETOKSIKAN AKUT TABLET EFFERVESCENT DARI EKSTRAK DAUN SIRIH (Piper betle L.) PADA TIKUS PUTIH JANTAN GALUR WISTAR Farida Hayati; Retno Murwanti; Dwi Brilyani Sandy
Jurnal Ilmiah Farmasi Vol. 4 No. 2 (2007)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTA research on acute toxicity of effervescent tablet from Piper betle leaf extractum on wistaralbino male rats was done. This research aimed to determine acute toxicity potential ofeffervescent tablet from piper betle leaf extractum, evaluated clinical symptom that perhaps occurdue to the giving of effervescent tablet, single dosage orally on 24 hours to 15 days observation.The research uses male rats, which are divided into 5 groups. Group I was negative control withaquadest. Group II was given sample with 0,38 g/kgBW dosage. Then, succesively group III with1,03 g/kgBW dosage, group IV with 2,79 g/kgBW dosage and group V was given test sample withhighest dosage, that was 7,50 g/kgBW dosage. On the basis of data analysis result having beenperformed both quantitively and qualitatively, it could be said that in general, the giving ofeffervescent tablet from Piper betle leaf extractum single dosage orally on male rats from 0,38g/kgBW dosage to highest level (7,50 g/kgBW) or approximately 19,74 times of therapy dosage,didn’t cause toxic effect. It didn’t cause mortality on research animal. So we can determine thequasi LD50, that was bigger than 7,50 g/kgBW, according to Loomis criteria (1978), that acutetoxicity potential of effervescent tablet from piper betle extractum was practically not toxic category.Key words: acute toxicity, effervescent tablet, Piper betle
UJI EFEK HEPATOPROTEKTIF INFUS HERBA PEGAGAN (Centella asiatica, (L.) Urb) PADA TIKUS PUTIH GALUR WISTAR YANG TERINDUKSI PARASETAMOL Islamiyah Neda Rahayu; Farida Hayati
Jurnal Ilmiah Farmasi Vol. 2 No. 1 (2005)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTAcetaminophen is a kind of analgetic-antipyretic drug which the usage in excessive dose willcause the liver damage. Pegagan herb (Centella asiatica, L) is one of plant which is used to protectthe liver from various damage of drug. A research to study the hepatoprotective effect of pegaganherb infusion on acetaminophen induced wistar rat has been conducted. This research was aimedto find how excellent pegagan herb protects the liver from the damages caused by acetaminophenand find the data of hepatoprotective of pegagan herb’s dose effect span in infussion form throughthe SGPT enzym and hyspatology of liver cell analysis. This research used the completed randomof unidirectoral pattern method, using wistar strain white rat as the tested animals, weight 200 g 10%, age 5-8 weeks. The way of attemp: 36 rat were divided into 6 groups were each groups had 6rat. For the treatment, Group I was controlled by aquadest. Group II was given acetaminophensuspention dose 2,5 g/kg BB. Group III-IV was given pegagan herb infussion 0,027; 0,054; 0,0108;0,216 g/kg BB once in a day for a week and 8 hours after that, on the seventh day was givenacetaminophen suspention dose 2,5 g/kg BB. Blood taking was conducted by orbitalis sinus beforeth treatment, 8 hours after the seventh day treatment and 24 hours after acetaminophen was given.SGPT data was analysed by ANOVA statistical test, where if found the significant difference, itwould continue by Tuckey Test with 95% as significant standar. Then continued by hyspatology teston liver cell of rat. The result of this research showed that pegagan herb infussion dose 0,027;0,054; 0,108; 0,216 g/kg BB had a hepatoprotective effect on acetaminophen inducted white ratsuccessively 34,19%; 62,52%; 78,62%; 85,69%. And the result of qualitative analysis by histologyanalysis showed that pegagan herb had hepatoprotective effect.Keyword: Hepatoprotective, Pegagan Herb,Acetaminophen
KEAMANAN PEMBERIAN BERULANG EKSTRAK KANGKUNG DARAT (Ipomoea reptans,Poir) TERSTANDAR TERHADAP FUNGSI GINJAL DAN HEPAR MENCIT BETINA Farida Hayati; Retno Murwanti; Ginna Zabrina
Jurnal Ilmiah Farmasi Vol. 10 No. 1 (2013): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol10.iss1.art1

Abstract

Kangkung darat terbukti memiliki aktifitas antihiperglikemia pada mencit betina galur swiss yang diinduksi streptozotosin. Penelitian ini dilakukan untuk mendapatkan kajian keamanan pemberian berulang ekstrak kangkung darat terstandar terhadap fungsi ginjal dan hepar pada mencit betina. Dua puluh hewan uji dibagi ke dalam 4 kelompok, yaitu kelompok kontrol (akuades 10 ml/ kg), dosis I (ekstrak etanolik kangkung darat 480 mg/ kg), dosis II (ekstrak etanolik kangkung darat 759 mg/ kg), dan  dosis III (ekstrak etanolik kangkung darat 1200 mg/ kg). Ekstrak etanolik kangkung darat diberikan 1 kali sehari secara p.o. selama 14 hari. Pengamatan gejala toksik dilakukan selama 3 jam setelah pemberian senyawa uji. Kelompok dosis 1200 mg/ kg mengalami efek sedasi, konstipasi, dan feses berwarna hitam selama pemberian ekstrak etanolik kangkung darat terstandar, sedangkan kelompok lainnya tidak mengalami gejala toksik. Data berat badan, pemeriksaan ALT, dan pemeriksaan AST dianalisis secara statistik. Berat badan rata-rata hewan uji kelompok dosis 759 mg/ kg mengalami penurunan yang paling banyak dibandingkan kelompok lainnya dan berbeda signifikan dengan kelompok kontrol dari hasil analisis statistik. Kadar AST dan ALT mengalami peningkatan setelah pemberian berulang ekstrak etanolik kangkung darat selama 14 hari, dari hasil analisis statistik kadar ALT dan AST kelompok dosis 759 mg/ kg dan dosis 1200 mg/ kg berbeda signifikan dengan kelompok kontrol (p<0,05). Hasil histopatologi organ ginjal dan hepar hewan uji setelah pemberian berulang ekstrak etanolik kangkung darat terstandar selama 14 hari menunjukkan tidak adanya perubahan yang membahayakan pada organ.
PENGARUH PRA-PERLAKUAN MADU TERHADAP FARMAKOKINETIKA ELIMINASI RIFAMPISIN PADA TIKUS WISTAR JANTAN Dimas Adhi Pradana; Farida Hayati; Dian Sukma
Jurnal Ilmiah Farmasi Vol. 10 No. 1 (2013): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol10.iss1.art3

Abstract

Rifampisin merupakan salah satu obat yang dipergunakan sebagai terapi lini pertama dalam pengobatan tuberkulosis. Tujuan dari penelitian ini untuk mengetahui pengaruh pra-perlakuan pemberian madu terhadap profil farmakokinetika fase eliminasi rifampisin pada tikus Wistar Jantan.  Dalam penelitian ini hewan uji dibagi menjadi 2 kelompok, yaitu kelompok kontrol dan perlakuan.  Setiap kelompok terdiri dari 5  ekor tikus.  Kelompok kontrol diberikan rifampisin dosis tunggal 50 mg/kg tikus sedangkan kelompok perlakuan diberikan madu 7,65 ml/kg secara oral sekali sehari selama 7 hari dan pada hari ke-8 diberikan rifampisin dosis 50 mg/kg BB tikus secara per oral. Sebanyak 0,2 ml sampel darah diambil dari vena lateralis ekor  pada 0.25; 0.5, 1.0, 1.5, 2.0, 3.0; 4.0; 6.0; 8, 0; 10.0; 12.0, dan 24.0 jam.  Penetapan kadar rifampisin dalam plasma dilakukan dengan metode HPLC pada panjang gelombang 244.6 nm. Parameter farmakokinetika fase eliminasi yang ditetapkan adalah k, t ½, dan ClT. Hasil penelitian menunjukkan bahwa pemberian pra perlakuan madu tidak mempengaruhi farmakokinetika fase eliminasi dari rifampisin. 
PENGARUH SUSU KEDELAI DOSIS 25 ml/kg BB TERHADAP BIOAVAILABILITAS TEOFILIN PADA TIKUS PUTIH JANTAN Farida Hayati; Arief Rahman Hakim
Jurnal Ilmiah Farmasi Vol. 3 No. 1 (2006)
Publisher : Universitas Islam Indonesia

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Abstract

Research has been conducted to study the effect of soya milk 25ml/kg BW per oral toward theophyline bioavailability when given concomittantly in male white rats. The study was done by using of wistar male white rats, weight 200-300 grams, age 3-4 month. The experimental design used was completely randomized design with two groups treatment, which each group test contained five rats. The first group (control) was given single dose pre-treatment of theophyline (25 ml/kg BW, orally), the second group (treatment) was given theophyline and soya milk (25 ml/kg BW, orally) with theophyline 25mg/kg BW. Blood samples were taken periodically from eye vein of rat in the 0,25; 1; 1,5; 3; 5; 6; 8; 10; and 24 hours. Determination of theophyline levels was done with HPLC by using Orcutt and friends method (1977) modified on the 269 nm wavelength. The concentration of theophyline was determined based on a standard curve. Theophyline bioavailability i.e. tmax, Cmax, and AUC0 inf was determined from the concentration to time data . Bioavailability parameters in the first group were tmax 2,36 ± 0,44 hours, Cmax 18,97± 2,06, AUC0 inf 231,48± 34,94 ug.hours/ml and bioavalibility parameters in the second group were tmax 2,08 ± 0,"l 7 hours, Cmax 24,11 ± 1,54, AUC0,nf 210,13±7,25 ug.hours/ml. The results indicated that soya milk was not found to be able to change theophyline bioavailability (P>0,05).
KETOKSIKAN AKUT TABLET EFFERVESCENT DARI EKSTRAK DAUM SIRIH ( Piper betle L.) PADA TIKUS PUTIH BETINA GALUR WISTAR Farida Hayati; Retno Murwanti; Wahyu Utaminingrum
Jurnal Ilmiah Farmasi Vol. 4 No. 1 (2007)
Publisher : Universitas Islam Indonesia

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Abstract

A research on acute toxicity of effervescent tablet from Piper betle leaf extractum on wistar albino female rats was done. This research aimed to determine acute toxicity potential of effervescent tablet from piper betle leaf extractum, evaluated clinical symptom that perhaps occur due to the giving of effervescent tablet, single dosage orally on 24 hours to 15 days observation. The research uses female rats, which are divided into 5 groups. Group I was negative control with aquadest. Group II was given sample with 0,38 g/kgBW dosage. Then, succesively group III with 1,03 g/kgBW dosage, group IV with 2,79 g/kgBW dosage and group V was given test sample with highest dosage, that was 7,50 g/kgBW dosage. On the basis of data analysis result having been performed both quantitively and qualitatively, it could be said that in general, the giving of effervescent tablet from Piper betle leaf extractum single dosage orally on female rats from 0,38 g/kgBW dosage to highest level (7,50 g/kgBW) or approximately 19,74 times of therapy dosage, didn't cause toxic effect which causing damage on lung, liver, kidney, heart, spleen, stomach and intestine. It didn't cause mortality on research animal. So we can determine the quasi LD^, that was bigger than 7,50 g/kgBW, according to Loomis criteria (1978), that acute toxicity potential of effervescent tablet from piper betle extractum was practically not toxic category. Thus based on ANOVA result with 95 % significance level, it could be said that the giving of effervescent tablet from piper betle leaf extractum didn't show significant differences to average body weight changes and organ weight.