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POTENSI KULTUR CAMPURAN BAKTERI ENDOFIT SEBAGAI PEMACU PERTUMBUHAN BIBIT TANAMAN KARET Hidayati, Umi; Chaniago, Iswandi Anas; Munif, Abdul; Siswanto, Siswanto; Santosa, Dwi Andreas
Jurnal Penelitian Karet JPK : Volume 32, Nomor 2, Tahun 2014
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v32i2.159

Abstract

Bakteri endofit adalah bakteri yang hidup dalam jaringan tanaman, dapat diisolasi melalui sterilisasi permukaan jaringan tanaman. Isolasi bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan sangat penting dilakukan. Pembuatan kultur campuran dari bakteri endofit diharapkan meningkatkan potensi dalam memacu pertumbuhan yang dapat meningkatkan kualitas bibit batang bawah tanaman karet. Penelitian ini dilakukan dengan tujuan mendapatkan kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Lima bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan yaitu Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, Brachybacterium paraconglomeratum LPD74, bacterium (bakteri tidak dikenal) LPD76, dan Providencia vermicola KPA38, diuji kompatibilitas untuk mendapatkan kultur campuran yang dapat meningkatkan pertumbuhan bibit batang bawah PB 260. Semua bakteri endofit terpilih kompatibel satu dengan yang lain. Aplikasi kultur campuran untuk meningkatkan pertumbuhan bibit batang bawah PB 260 memberikan hasil 2 kultur campuran terbaik. Kultur campuran 1 terdiri 2 spesies bakteri yaitu Brachybacterium paraconglomeratum LPD74 dan Providencia vermicola KPA38.  Kultur campuran 2 terdiri 3 spesies bakteri yaitu  Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, dan Brachybacterium paraconglomeratum LPD74. Bakteri endofit mampu masuk ke planlet bibit karet microcutting yang dibuktikan dengan Scanning Electron Microscopy. Diterima : 19 Mei 2014; Direvisi : 30 Mei 2014; Disetujui : 21 Juni 2014  How to Cite : Hidayati, U., Chaniago, I. A., Munif, A., Siswanto., & Santosa, D. A. (2014). Potensi kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Jurnal Penelitian Karet, 32(2), 129-138. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/159
PEMANFAATAN KONSORSIUM BAKTERI LOKAL UNTUK BIOREMEDIASI LIMBAH TEKSTIL MENGGUNAKAN SISTEM KOMBINASI ANAEROBIK-AEROBIK Sastrawidana, I Dewa K; Lay, Bibiana W; Fauzi, Anas Miftah; Santosa, Dwi Andreas
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1086.061 KB) | DOI: 10.14203/beritabiologi.v9i2.2020

Abstract

The objective of this research is to study the potential use microorganisms which are identified as Aeromonas sp., Pseudomonas $p., Flavobaclerium sp., Plesiomonas sp. and Vibrio sp. Five bacteria strains from sludge of Badung river were identified as Vibrio sp. and Plesiomonas sp. Two anaerobic-aerobic reactors were operated to treat textile waste water. Each reactor contained vulcanic stone to increase spesific surface of media for attachment of bacteria.Bacteria consortia used for anaerobic process consist of Aeromonas sp. (ML6), Aeromonas sp. (ML14), Aeromonas sp. 9ML24), Pseudomonas sp. (ML8) and Flavobacterium sp. (ML20). Whereas, bacteria consortia for aerobic process consist of Plesiomonas sp. (SB1), Plesiomonas sp. (SB2), Vibrio sp.(SB1), Vibrio sp. (SB2) and Vibrio sp. (SB3). The system was operated for 3 day in each reactor. The result showed, biodegradation of textile waste water in combined anaerobic-aerobic system by attached growth process is potential for treatment of textile waste water.This technology is effective to decrease COD value up to 98.38%, BODS 93.90%, TDS 80.87%, TSS 87.50% and decolorization of textile dyes up to 95.57%.
Aplikasi Konsorsium Mikrob Filosfer dan Rizosfer Untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Padi Application of Phyllosphere and Rhizosphere Microbial Consortium to Improve Rice Growth and Production Aksarah Pas, Aris; Sopandie, Didy; Trikoesoemaningtyas, Trikoesoemaningtyas; Santosa, Dwi Andreas Santosa
JURNAL PANGAN Vol 24, No 1 (2015): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.849 KB) | DOI: 10.33964/jp.v24i1.39

Abstract

Pendekatan secara biologi, memanfaatkan konsorsium mikrob filosfer dan mikrob rizosfer merupakan langkah alternatif mengurangi dampak negatif penggunaan pupuk sintetik, untuk mencapai produksi padi yang tinggi. Penelitian ini bertujuan untuk mempelajari peran konsorsium mikrob filosfer dan mikrob rizosfer terhadap pertumbuhan dan hasil tanaman padi. Penelitian dilakukan mulai bulan Januari hingga Mei 2014, di Green House Indonesian Centre For Biodiversity and Biotecnology, Bogor. Dalam penelitian ini digunakan konsorsium mikrob terbaik hasil seleksi, yaitu konsorsium mikrob filosfer Fm48 dari daun tumbuhan Emmerrilia ovalis Miq Dandy dan konsorsium mikrob rizosfer R15 dari rizosfer tumbuhan Physalis angulata L. Penelitian menggunakan Rancangan Acak Kelompok (RAK) satu faktor, yang terdiri atas tiga perlakuan, yaitu : pemberian pupuk N sintetik setengah dosis anjuran yaitu 30 N/ha, pemberian pupuk N sintetik sesuai dosis anjuran, yaitu 60 kg N/ha dan pemberian kombinasi konsorsium mikrob filosfer Fm48 dan mikrob rizosfer R15 dengan diberi pupuk N sintetik setengah dosis anjuran. Hasil penelitian menunjukkan bahwa, aplikasi kombinasi konsorsium mikrob dengan pemberian pupuk N sintetik setengah dosis anjuran, meningkatkan jumlah anakan, bobot kering tanaman, jumlah anakan produktif dan bobot segar malai setara dengan pemberian pupuk N sintetik sesuai dosis anjuran. Biological approach, by utilizing phyllosphere and rhizosphere microbial consortium, offers n alternative method to avoid the negative impact of synthetic fertilizer to the environment to achieve higher rice production. This research aims to study the role of phyllosphere and rhizosphere microbial consortium on the growth and yield of rice plants. The study was conducted from January to May 2014 in Green House Indonesian Centre For Biodiversity and Biotechnology, Bogor. Microbial consortium of phyllosphere Fm48 from plant leaves Emmerrilia ovalis Miq Dandy and microbial consortium of rhizosphere R15 from plant rhizosphere Physalis angulata L. are selected for this study. This research is designed in randomized block design with one factor, which consists of three treatments, namely: half recommended dose of synthetic N fertilizer (30 kg N/ha), recommended dose of Synthetic N fertilizer (60 kg N/ha), and a combination of microbial consortium of phyllosphere and rhizosphere microbes plus half recommended dose of synthetic N fertilizer. The results show that the applications of microbial consortium combined with half of recommended dose of synthetic N fertilizer, increases the number of tillers, plant dry weight, number of productive tillers, and panicles fresh weight equivalent to the use of recommended dosage of synthetic N fertilizer. 
APLIKASI MIKROORGANISME LOKAL (MOL) DIPERKAYA MIKROB BERGUNA PADA BUDIDAYA PADI SYSTEM OF RICE INTENSIFICATION (SRI) ORGANIK Batara, Lily Noviani; Anas, Iswandi; Santosa, Dwi Andreas; Lestari, Yulin
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) Vol 40, No 1 (2016)
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v40n1.2016.71-78

Abstract

Abstrak. Mikroorganisme Lokal (MOL), suatu cairan bahan organik yang ditambahkan gula merah atau molase, berperan untuk meningkatkan pertumbuhan dan produksi serta mengatasi masalah hama dan penyakit tanaman padi pada System of Rice Intensification (SRI) organik. Sifat MOL sangat beragam dan sering tidak mengandung mikrob berguna. Tujuan penelitian ini adalah untuk (i) mengevaluasi kualitas berbagai macam MOL, (ii) memperbaiki kualitas MOL dengan menambahkan mikrob berguna, serta (iii) menguji pengaruh MOL yang diperbaiki kualitasnya terhadap pertumbuhan dan produksi tanaman padi dengan metode SRI organik. Pengujian kualitas dan perbaikan kualitas MOL dilakukan di Laboratorium dan di lapang di Desa Ciasihan, Kecamatan Pamijahan, Kabupaten Bogor, Jawa Barat. Mikrob berguna yang digunakan untuk memperbaiki kualitas MOL yaitu Azotobacter sp., Azospirillum sp., bakteri pelarut fosfat, dan Trichoderma harzianum. Hasil penelitian menunjukkan bahwa kualitas MOL yang diproduksi oleh petani sangat beragam sifat fisik, kimia dan biologinya. Kandungan unsur hara N paling tinggi terdapat pada MOL krokot 0,15%, unsur hara P pada MOL krokot dan nasi 0,06%, sementara unsur hara K pada MOL rebung 0,63%. Pembuatan MOL secara kuantitatif dan penambahan mikrob berguna ke dalam MOL mampu meningkatkan kualitas MOL yang dapat dilihat dari peningkatan pertumbuhan dan hasil tanaman padi. Jumlah anakan MOL rebung diperkaya mikrob berguna 42 batang rumpun-1 lebih tinggi bila dibandingkan dengan perlakuan MOL rebung 39 batang rumpun-1. Jumlah gabah dengan MOL rebung diperkaya mikrob berguna rata-rata 148,5 gabah malai-1, lebih tinggi dibandingkan dengan MOL rebung 142,5 gabah malai-1. Berat gabah kering panen MOL rebung diperkaya mikrob 10,7 t ha-1, lebih tinggi bila dibandingkan dengan MOL rebung setinggi 9,3 t ha-1.Abstract. Indigenous Microbes (IMO), an organic liquid product enriched with palm sugar or molasses, can be used to improve the growth and yield as well as to protect plants from pest and diseases in the Organic System of Rice Intensification (SRI). IMO characteristics vary and some do not contain beneficial microbes. This research was aimed to (i) evaluate the quality of IMO, (ii) to improve IMO quality by enriching with beneficial microbes and (iii) to evaluate the effects of enriched IMO on rice growth and yield under the Organic SRI. Chemical, physical and biological properties of IMO were evaluated at the Laboratory and the field trial was performed at Ciasihan village, Pamijahan District, Bogor, West Java. Benefical microbes used to improve the quality of IMO were Azotobacter sp., Azospirillum sp., phosphate solubilizing bacteria and Trichoderma harzianum. The results of this study showed that the physical, chemical and biological properties of IMO produced by farmers varied. Purslane IMO was highest in N content (0.15%), P content in purslane and rice IMO was 0.06% while K content in bamboo shoot IMO was 0.63%. Quantitative preparation of IMO and enrichment with beneficial microbes is necessary to improve its quality as can be observed from the improvement of rice growth and yield. Numbers of tillers of bamboo shoots of IMO enriched with beneficial microbes was 42 tillers hill and was higher compared to the treatment of bamboo shoots IMO without microbe enrichment which was 39 tillers hill-1. The number of rice grain under bamboo shoot IMO enriched beneficial microbes was 148.5 grains panicle-1 which was higher than that of the bamboo shoot IMO without enriched beneficial microbes of 142.5 grains panicle-1. Grain yield under bamboo shoot IMO enriched beneficial microbes was also higher, i.e. 10.7 t ha-1, which was higher compared to bamboo shoots IMO without enriched beneficial microbes of 9.3 t ha-1.
Deagrarianization and Local Food Security Strategy for Peasant Communities in Rural Central Java Pujiriyani, Dwi Wulan; Soetarto, Endriatmo; Santosa, Dwi Andreas; Agusta, Ivanovich
KOMUNITAS: International Journal of Indonesian Society and Culture Vol 11, No 2 (2019): September
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/komunitas.v11i2.20209

Abstract

Food security is one of the important issues in the study of deagrarianization. In macro level, deagrarianization can weaken food security which is indicated by the ability of food production which continues to decline due to the structural transformation of the economy from agriculture to non-agriculture. The purpose of this study is to determine food security at the community level when the symptoms of macro deagrarianization have occurred. This research is conducted by qualitative methods. An ethnosociological approach is used by combining community case study methods and ethnographic methods. The results show that food security at the community level is still well-maintained. Deagrarianization has not diminished the ability of the community to meet their food needs. Communities have internal mechanisms that secure their food sufficiency through food strategies and non-food strategies. The food strategy is carried out through saving yields for grain stores (family food barns) and reduce the amount of consumption. Meanwhile the non-food strategy is carried out through diversification of livelihoods by relying on available extractive landscapes. Swamps (balongan) are used as a food source that provides various types of local fish, crabs and snails as a source of protein for family consumption and also for sale to local collectors.
KEANEKARAGAMAN ENZIM INVERTASE, PENGEMBANGAN STRAIN UNGGUL DAN TEKNOLOGI PRODUKSINYA Sanjaya, Wilhelmus Terang Arga; Giyanto, .; Widyastuti, Rahayu; Santosa, Dwi Andreas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1328.71 KB) | DOI: 10.29122/jbbi.v7i1.3705

Abstract

Invertase Diversity, Novel Strain and Production Technology DevelopmentInvertase or β-fructofuranosidase (E.C.3.2.1.2.6) have been a valuable enzyme in food industry, so that research about enhancing invertase activity on an industrial scale has been reported massively. So far, the production of the invertase enzyme is highly dependent on the potential activity of the invertase enzyme derived from microorganisms. The development of enzyme production technology is also a concern in invertase research to obtain an efficient and inexpensive production system. So far, various developments in enzyme production technology and enzyme utilization have been carried out, including various innovations in immobilizing and increasing the stabilization of the invertase enzyme during the production process. The development of superior strains and enzyme production technology continues amid the discovery of the latest approaches such as genetic engineering, protein modification, and nanotechnology. This paper aims to discuss invertase variation from various organisms, its correlation to novel microbial strain development to increase invertase production and invertase enzyme production technologies development including immobilization technology and stabilization of invertase enzymes.Keywords: β-fructofuranosidase, ezyme production, fermentation, immobilitation, invertase geneABSTRAKInvertase atau β-fructofuranosidase (E.C.3.2.1.2.6) merupakan enzim yang sangat penting dalam dunia industri pangan sehingga berbagai optimasi aktivitas enzim untuk produksi dalam skala industri terus dilakukan. Produksi enzim invertase hingga sejauh ini sangat bergantung oleh potensi aktivitas enzim invertase yang diambil dari mikroorganisme. Selain itu, pengembangan teknologi produksi juga menjadi perhatian dalam pengembangan produksi dan pemanfaatan enzim untuk mendapatkan sistem produksi yang efisien dan murah. Sejauh ini berbagai pengembangan teknologi produksi enzim dan pemanfaatan enzim telah dilakukan, diantaranya dengan berbagai inovasi dalam melakukan imobilisasi dan peningkatan stabilisasi enzim invertase selama proses produksi. Pengembangan strain unggul dan teknologi produksi enzim terus dilakukan di tengah ditemukannya pendekatan-pendekatan terkini seperti rekayasa genentika, modifikasi protein, dan teknologi nano. Artikel ini bertujuan untuk membahas variasi enzim invertase dari berbagai organisme, korelasi pengembangan strain mikrobia unggul terhadap peningkatan produksi enzim invertase, dan perkembangan teknologi produksi enzim invertase meliputi teknologi imobilisasi dan stabilisasi enzim invertase.
The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi Widrializa, .; Widyastuti, Rahayu; Santosa, Dwi Andreas; Djajakirana, Gunawan
JOURNAL OF TROPICAL SOILS Vol 20, No 3: September 2015
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2015.v20i3.173-180

Abstract

Harapan forests is the first restoration forest in Indonesia, includes several different ecosystems. Different ecosystems have different characteristic to affect the diversity and abundance of Collembola. In the ecosystem, Collembola have an important role in biocontrol, decomposition, soil nutrient distribution, stimulation of soil microbial activity and as an alternative feed for predators. This study was aimed to investigate the diversity and abundance of Collembola in four ecosystems at Harapan forest, i.e. secondary forest, rubber forest, rubber smallholder and oil palm smallholder. To achieve the objective, soil samples were taken at 48 observation points in the four ecosystem. The soil samples were then extracted by Kempson Extractor. The diversity and abundance of Collembola in four ecosystems were categorized as medium to high. The total of individual and population of Collembola in the secondary and rubber forest ecosystems were likely to be higher than in the rubber and oil palm smallholders. This study had also identified four orders, 14 families and 31 genera of Collembola, where Isotomiella and Proisotoma dominated the genus of Collembola in the four ecosystems. [How to Cite: Widrializa, R Widyastuti, DA Santosa and G Djajakirana. 2015. The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi. J Trop Soils 20: 173-180. Doi: 10.5400/jts.2015.20.3.173] 
Seleksi dan Identifikasi Bakteri Antagonis sebagai Agens Pengendali Hayati Penyakit Hawar Pelepah Padi Rustam, Rustam; Giyanto, Giyanto; Wiyono, Suryo; Santosa, Dwi Andreas; Susanto, Slamet
Jurnal Penelitian Pertanian Tanaman Pangan Vol 30, No 3 (2011): Desember 2011
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.967 KB) | DOI: 10.21082/jpptp.v30n3.2011.p164-171

Abstract

Selection and identification of effective microbes are important steps to obtain biological control agents. The objective of this research was to screen potential bacteria as controlling agents for rice sheath blight disease. The research was conducted at plant bacteriological laboratory and green house of Plant Protection Division of Bogor Agricultural University, Bogor, from May 2010 to February 2011. The trial was arranged in a completely randomized design with bacterial isolates as treatment. The result showed that 30 out of 144 bacterial isolates indicated an antifungal activity to R. solani. In vivo test indicated that 3 of the 30 isolates which have antifungal activity were able significantly to suppress the rice sheath blight disease. Those isolates were marked as TT47, SS19 and BR2, with the ability to suppress rice sheath blight disease at rate of 79.6, 56.4, and 49.4%, disease index 1.7, 3.7, and 4.3, and the disease incidence 33.3%, 73.35, and 80%, respectively. Molecular characterization of partial sequence of 16S rRNA on SS19, TT47, and BR2 isolates showed that those bacteria are Serratia marcescens, Ralstonia pickettii, and Bacillus subtilis, respectively.
KEANEKARAGAMAN ENZIM INVERTASE, PENGEMBANGAN STRAIN UNGGUL DAN TEKNOLOGI PRODUKSINYA Wilhelmus Terang Arga Sanjaya; . Giyanto; Rahayu Widyastuti; Dwi Andreas Santosa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1328.71 KB) | DOI: 10.29122/jbbi.v7i1.3705

Abstract

Invertase Diversity, Novel Strain and Production Technology DevelopmentInvertase or β-fructofuranosidase (E.C.3.2.1.2.6) have been a valuable enzyme in food industry, so that research about enhancing invertase activity on an industrial scale has been reported massively. So far, the production of the invertase enzyme is highly dependent on the potential activity of the invertase enzyme derived from microorganisms. The development of enzyme production technology is also a concern in invertase research to obtain an efficient and inexpensive production system. So far, various developments in enzyme production technology and enzyme utilization have been carried out, including various innovations in immobilizing and increasing the stabilization of the invertase enzyme during the production process. The development of superior strains and enzyme production technology continues amid the discovery of the latest approaches such as genetic engineering, protein modification, and nanotechnology. This paper aims to discuss invertase variation from various organisms, its correlation to novel microbial strain development to increase invertase production and invertase enzyme production technologies development including immobilization technology and stabilization of invertase enzymes.Keywords: β-fructofuranosidase, ezyme production, fermentation, immobilitation, invertase geneABSTRAKInvertase atau β-fructofuranosidase (E.C.3.2.1.2.6) merupakan enzim yang sangat penting dalam dunia industri pangan sehingga berbagai optimasi aktivitas enzim untuk produksi dalam skala industri terus dilakukan. Produksi enzim invertase hingga sejauh ini sangat bergantung oleh potensi aktivitas enzim invertase yang diambil dari mikroorganisme. Selain itu, pengembangan teknologi produksi juga menjadi perhatian dalam pengembangan produksi dan pemanfaatan enzim untuk mendapatkan sistem produksi yang efisien dan murah. Sejauh ini berbagai pengembangan teknologi produksi enzim dan pemanfaatan enzim telah dilakukan, diantaranya dengan berbagai inovasi dalam melakukan imobilisasi dan peningkatan stabilisasi enzim invertase selama proses produksi. Pengembangan strain unggul dan teknologi produksi enzim terus dilakukan di tengah ditemukannya pendekatan-pendekatan terkini seperti rekayasa genentika, modifikasi protein, dan teknologi nano. Artikel ini bertujuan untuk membahas variasi enzim invertase dari berbagai organisme, korelasi pengembangan strain mikrobia unggul terhadap peningkatan produksi enzim invertase, dan perkembangan teknologi produksi enzim invertase meliputi teknologi imobilisasi dan stabilisasi enzim invertase.
KEMAMPUAN BAKTERI DARt EKOSISTEM AIR HITAM KALIMANTAN TENGAH DALAM MEROMBAK MINYAK BUMI DAN SOLAR Iswandi Anas; Noegroho Hadi; Dwi Andreas Santosa
Jurnal Ilmu Tanah dan Lingkungan Vol 2 No 2 (1999): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.703 KB) | DOI: 10.29244/jitl.2.2.1-7

Abstract

The objective of this experiment was to evaluate the abili!y of bacteria strains isolated from black water ecosystem of Central Kalimantan in degrading crude oil and diesel oil. The experiment was conducted at the Laboratory of Soil Biology, Department of Soil Sciences, Faculty of Agriculture, Bogor Agricultural University (IPB). The crude oil degrading ability of bacteria was tested on a minimum liquid medium (Gurujeyalakshmi dan Orial, 1989) and soil. Six crude oil and diesel oil degrading bacteria were isolated. The bacteria were identified as Bacillus panthotenticus (2 strains), B. circulars (1 strain), Pssudomonas diminuta (1 strain), P. stufzeri (1 strain) and Klebsiella edwardsii (1 strain). These bacteria were able to degrade crude oil or diesel oil whether in a liquid minimum medium or in soil.On a minimum liquid medium, after 10 days incubation, bacteria were able to degrade 20.49% of the crude oil, while in the Entisol soil, after 7 days of incubation, as high as 40.29% of crude oil was degraded and after 28 days incubation, 64.95 of crude oil had been degraded by selected bacteria.
Co-Authors . Giyanto . Giyanto Abdul Hasyim Sodiq Abdul Munif Agus Purwito Aisamrotul Hasanah Aksarah Pas, Aris Amanda Kusuma Dewi Anas Miftah Fauzi Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo Atang Sutandi Batara, Lily Noviani Bedah Rupaedah Bibiana W Lay Bibiana W Lay BIBIANA W LAY Chansyanah Diawati Dedeh H Arief Dedi Widayat Dian Hendrayanti Didiek Hadjar Goenadi Didy Sopandie Dwi Wulan Pujiriyani Endriatmo Soetarto F. Dian Lina Fahrizal Hazra Firdaus Alam Hudi Giyanto, Giyanto Gunawan Djajakirana Gustav Adolf Wattimena Hamim Hamim HANNA ARTUTI EKAMAWANTI Hari R Harlyadi Harpasis S. Sanusi Harpasis S. Sanusi Haryanti Haryanti Hengki Tornando I Dewa Ketut Sastrawidana Ilyas Jamil Iman Rusmana Indriyani, Yohanna Anisa Iskandar Iskandar Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Iswandi Anas Chaniago, Iswandi Anas Ivanovich Agusta Juhari Juhari, Juhari Khoirunnisa, Nur Syafira Kukuh Murtilaksono Kurnia Dewi Sasmita Kusuma Handayani Laksmita Prima Santi Laksono Trisnantoro LILIK BUDIPRASETYO Maggy T Suhartono Memen Surahman Mieke Rochimi Setiawati Miftahudin Muchamad Yusron Muhammad Rivai Nasrul Harahap NISA RACHMANIA MUBARIK Noegroho Hadi Noor Fadiawati NUNI GOFAR Nursaadah . RAHAYU WIDYASTUTI Rani Nur Fitriani Remaja Sitepu Rustam, Rustam Ruyitno Nuchsin Ruyitno Ruyitno S Siswanto Sanjaya, Wilhelmus Terang Arga Siswanto siswanto Slamet Susanto Sri Wilarso Budi R Sudarsono Sukmadewi, Desak Ketut Tristiana SULASTRI Suryo Wiyono Susiyanti . Syaiful Anwar T W DARMONO Titik Tri Wahyuni Tommy Kurniawan Tri Prartono Trikoesoemaningtyas Tualar Slmarmata Umi Hidayati Umi Hidayati Untung Sudadi Wahono Sumaryono Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya YADI SETIADI Yeti Darmayati Yeti Darmayati Yohanna Anisa Indriyani YULIN LESTARI Yuni Lisafitri Yuyun Sumarni Zulfarina Zulfarina Zulfarina,