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All Journal Makara Journal of Science Molekul: Jurnal Ilmiah Kimia
Sari, Aprilia Permata
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Diketopiperazine Cyclo-(S-Pro-R-Leu) Produced by Periconia pseudobyssoides K5 Isolated from Toona sureni (Meliaceae) and its Heme Polymerization Inhibition Activity Azhari, Azmi; Harneti, Desi; Wulandari, Asri Peni; Mulyani, Yeni; Purbaya, Sari; Sari, Aprilia Permata; Pratama, Galih Bayu; Supratman, Unang; Shiono, Yoshihito
Makara Journal of Science Vol. 27, No. 1
Publisher : UI Scholars Hub

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Abstract

Fungi endophytes are living microorganisms colonizing inside the internal tissue of a plant and provide ecological benefits for their host. Endophytes provide various metabolites for plant adaptation toward biotic and abiotic stresses and have tremendous pharmacological activities. Toona sureni (T. sureni) (Meliaceae) belongs to the Toona genera and is reported to have antimalarial activity. To determine the compounds produced by the endophytic fungus from this plant, we isolated a compound from Periconia pseudobyssoides (P. pseudobyssoides) K5 endophytes from the stem bark of T. sureni. Diketopiperazine cyclo-(S-Pro-R-Leu), a non-ribosomal peptide, was isolated from brown rice fermented at 28 ± 2 ℃ for 30 days. The structure was determined by spectroscopic methods including fourier-transform infrared spectroscopy, mass spectrometry, and 1D and 2D nuclear magnetic resonance techniques. This compound was evaluated for heme polymerization inhibition activity (HPIA) with an IC50 value of 9.89 ± 0.24 mmol/L compared with positive control chloroquine phosphate with an IC50 value of 3.08 ± 0.58 mmol/L. This compound has been categorized as having low activity three times lower than positive control chloroquine phosphate. This information provides new leads about the compound diketopiperazine cyclo-(S-Pro-R-Leu) produced by P. pseudobyssoides K5 endophytes having low activity in inhibiting heme polymerization. In the future, to explore the potency of this compound as antimalarial agent, the other antimalarial test such as lactate dehydrogenase assay might be useful.
Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines Supratman, Unang; Purbaya, Sari; Harneti, Desi; Wulandari, Asri Peni; Mulyani, Yeni; Azhari, Azmi; Sari, Aprilia Permata
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.7824

Abstract

Three steroids, ergosterol (1), ergosterol peroxide (2) and stigmasterol (3) have been isolated from endophytic fungus, Lasiodiplodia theobromae derived from the root of Aglaia argentea Blume. The steroids were isolated by vacuum chromatography and column chromatography, the chemical structure was established following the analysis of 1D-NMR, 2D-NMR, IR, MS and by comparison with previously reported spectra data. Ergosterol peroxide (2) and stigmasterol (3) were reported for the first time isolated from Lasiodiplodia theobromae endophytic fungus. Cytotoxic activities of the compounds were tested with resazurin assay against HeLa cervical cancer cells, compound 2 displayed strongest cytotoxic activities against HeLa cervical cancer cells with IC50 values of 0.28 µM, while compounds 1 and 3 showed IC50 values of 0.34 µM and 27.32 µM, respectively
Steroids Produced by Endophytic Fungi (Fusarium phaseoli) Isolated from Chisocheton macrophyllus and their Antibacterial Activity against Escherichia coli and Staphylococcus aureuss Katja, Dewa Gede; Sari, Aprilia Permata; Sinaga, Siska Elisahbet; Nurlelasari, Nurlelasari; Farabi, Kindi; Sofian, Ferry Ferdiansyah; Fajriah, Sofa; Naini, Al Arofatus; Supratman, Unang
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.12727

Abstract

ABSTRACT. Steroids are secondary metabolic derivatives of terpenes containing the tetracyclic ring system known to exhibit fascinating pharmacological activity. Steroids are distributed in various genera of endophytic fungi including Fusarium genus which lives inside a higher tree such as Chisocheton macrophyllus. The purpose of this research is to identify and characterize the chemical structure of steroids generated by F. phaseoli, an endophytic fungus obtained from C. macrophyllus roots, as well as to assess their antibacterial activity against Escherichia coli and Staphylococcus aureus. The brown rice medium was fermented with F. phaseoli for six weeks before extraction with ethyl acetate. The extracts yielded four compounds, identified using spectroscopic methods such as FTIR, HRTOF-MS, 1D, and 2D NMR, and then compared to previously described compounds. Compounds 1-4 were identified as ergosterol (1), ergosterol peroxide (2), atroside (3), and cerevisterol (4). The four isolated compounds were evaluated for antibacterial activity against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 11229 and displayed activity with MIC50 values of 500 µg/mL. Keywords: Antibacterial activity; Chisocheton macrophyllus; Fusarium phaseoli; steroids.
Co-Authors Asri Peni Wulandari, Asri Peni Azmi Azhari, Azmi Desi Harneti Putri Huspa Dewa Gede Katja Farabi, Kindi Ferry Ferdiansyah Sofian, Ferry Ferdiansyah Naini, Al Arofatus Nurlelasari Nurlelasari Pratama, Galih Bayu Purbaya, Sari Shiono, Yoshihito Sinaga, Siska Elisahbet Sofa Fajriah Unang Supratman Yeni Mulyani