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All Journal Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Biologi Universitas Andalas Jurnal Biologi Tropis Sains Natural: Journal of Biology and Chemistry Menara Perkebunan Unri Conference Series: Community Engagement Dinamika Pertanian
Mayta Novaliza Isda
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Immunology & microbiology Mathematics

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In Vitro Callus Induction in Tacca (Tacca chantrieri Andre) Leaf Explants on Murashige and Skoog Media with Different Concentrations of Sucrose Mayta Novaliza Isda; Melda Jannatul Salsabilla
Jurnal Biologi Universitas Andalas Vol 10, No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.1-9.2022

Abstract

Tacca chantrieri belongs to the family Taccaceae has black flowers and has a long filiform that looks like a bat. T. chantrieri contains phytochemicals in the form of spritosol saponins used as traditional medicine by the people of China and Thailand. The amount of land clearing, forest exploitation and habitat destruction resulted in a reduction in the number of T. chantrieri, so T. chantrieri was propagated to maintain its sustainability. One way that can be used is the in vitro culture technique, namely callus culture. Callus culture is an early stage of in vitro culture technique where this stage aims to produce and multiply callus cells. The purpose of the study is were to determine the effect of different sucrose concentrations on callus induction from T. chantrieri leaf explants and determine the best sucrose concentrations for callus culture from T. chantrieri leaf explants on Murashige and Skoog (MS) media. This study used a single factor completely randomized design (CRD), namely sucrose concentrations 0 g/l, 10 g/l, 20 g/l, 30 g/l, 40 g/l, 50 g/l with five replications. The results of this study showed that the administrations of sucrose with different concentrations on MS media had an effect on increasing callus induction in Tacca chantrieri leaves. The best sucrose concertations for callus induction of Tacca chantrieri leaves was the addition of 40 g/l sucrose at 20 days after planting, 60 % callus formation percentage, callus formed in the form of compact callus and produce yellow-white callus. Keywords: Tacca chantrieri, sucrose, callus, Murashige and Skoog.
INDUKSI TUNAS IN VITRO JERUK SIAM (Citrus nobilis Lour.) ASAL KAMPAR DARI EKSPLAN TUNAS APEKS DAN NODUS IN VITRO Nurul Hidayati; Wahyu Lestari; Mayta Novaliza Isda
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Citrus nobilis Lour. is a citrus plant that is highly demanded by many people in Riau. Propagation of C. nobilis Lour. has some problems due to various diseases that decrease its production. The conventional propagation of C. nobilis. requires a lot of parent plants and takes a long time, therefore the in vitro propagation is necessary. The purpose of this study was to determine the concentration of BAP and explant source as well as the interaction between both treatments in inducing C. nobilis bud. This research used a factorial randomized block design (RBD) with two factors i.e the concentration of BAP (0, 1, 2 and 3 mg / l) and the source of explants (shoot apex and 6 week in vitro nodes). All combined treatments showed no significant difference to the emerging bud, the number of shoots and length of shoot. The results showed that the addition of 2 mg/l BAP at the apex shoot and node explants were able to induce bud. Better explant sources in inducing bud was the node explants. The node explants at a concentration of 1 mg/l BAP gave better result in inducing bud.
UJI EFEKTIVITAS BAKTERI PELARUT FOSFAT PENGHASIL ASAM SIANIDA ASAL TANAH GAMBUT RIAU DALAM MENGENDALIKAN GULMA DOMINAN PADA TANAMAN KELAPA SAWIT Tri Septiani; Delita Zul; Mayta Novaliza Isda
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

This research aimed to analyze the effectiveness of phosphate solubilizing bacteria (PSB) which are able to produce cyanide acid (HCN) in controlling of predominant weed in the palm oil plantation. This research was conducted from February to July 2014 in the Laboratory of Microbiology, Faculty of Mathematics and Natural Science, University of Riau. The effectiveness of PSB in controlling the growth of Mikania micrantha was performed by inoculating 26 selected PSB isolates into pre-germinated weed based on the Kremer and Souissi method modification. The parameters observed were root length and shoot length of seedling weed. The results showed that PSB isolates tested, in general, were effective to inhibit the growth of root and shoot length of the weed. BB_K9 isolate was the most effective in controlling the weed as it had a strong level of HCN production.
INDUKSI TUNAS JERUK SIAM (Citrus nobilis Lour.) ASAL KAMPAR DENGAN PEMBERIAN BENZIL AMINO PURINE (BAP) SECARA IN VITRO Rahmahayu '; Siti Fatonah; Mayta Novaliza Isda
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Citrus (Citrus nobilis Lour.) from Kampar is one of primary commodities from Riau province. However, virus attacks cause citrus plants become sick and even died, therefore it is difficult to find the healthy citrus plants. In order to recover this condition, it is necessary to provide citrus seedlings in large quantities. One alternative to provide citrus seedling in large quantities is through tissue culture technique (in vitro culture). This study aimed to find out the effect of benzil amino purine (BAP) and to determine the optimal concentration of BAP in inducing shoot of citrus. This experiment was designed using a randomized block design (RBD) with various concentrations of BAP. The results showed that the treatment with 1 mg/l BAP was the best in inducing the number of shoots (3 shoots/explant) and treatment with 0.5 mg/l BAP was the best in generating shoot length (3.11 cm) and number of leaves (5.26 piece/shoot).
INDUKSI AKAR JERUK SIAM ASAL KAMPAR (Citrus nobilis Lour.) DARI TUNAS IN VITRO DENGAN BERBAGAI KOMBINASI SUKROSA DAN NAA PADA MEDIA ½ MURASHIGE AND SKOOG Indah Wijayanti; Mayta Novaliza Isda; Wahyu Lestari
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

> Citrus (Citrus nobilis Lour.) from Kampar is one of primary commodity from Riau Province. In order to conserve citrus from Kampar as a main fruit from Riau, it is necessary to carry out seedling multiplication using in vitro method. The purpose of the study was to determine the best concentration of sucrose and NAA as well as to determine their interaction in inducing citrus root on ½ MS media. This research used Randomized Block Design (RBD) factorial with two factors, i.e. the concentration of sucrose (1, 2, 3 and 4%) and NAA (0, 0,5, 1 and 1 ,5 mg/l). The concentration of 1 and 2% sucrose produced the highest number of root (1,17 units), 2% sucrose could give the fastest root induction (17,6 days) and also produced the longest root (2,57 cm). The combination of 1% sucrose + 1 mg/l NAA could give the fastest root induction (11,5 days), while the combination of 3% sucrose + 0,5 mg/l NAA produced the longest root (2,31 cm) and 3% sucrose + 1,5 mg/l NAA produced the highest number of root (1,64 units).
POTENSI ALELOPATI EKSTRAK DAUN Gleichenia linearis (Burm.) Underw. TERHADAP PERKECAMBAHAN DAN PERTUMBUHAN ANAKAN GULMA Mikania micrantha (L.) Kunth Awit Tatas Asih Susanti; Mayta Novaliza Isda; Siti Fatonah
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Gleichenia linearis is a wide-leaf weed that contains chemical compounds such as flavonoid, allelochemical, triterpenoid, saponin, tannin, alkaloid and steroid that can inhibit the growth of other plants so it can be used as bioherbicide. The aims of this study were to determine the potential of residues and the concentration of G. linearis leaf extract on germination and growth of seedling weed Mikania micrantha. This studywas conducted using completely randomized design with 5 replicates with three concentrations i.e. 10%, 20% and 30%. Data was observed and analyzed using Analysis of Variance (ANOVA) and tested using Duncan's Multiple Range Test (DMRT) if it showed significant effect. This study showed the decrease of growth and germination and the increase of the percentage of the death of M. micrantha weed after treatment of G. linearis leaf extract. The highest decrease was found in 30% extract concentration with the percentage of germination, fresh weight, the percentage of mortality were 19%, 0.436 g and 10%, respectively.
ANALISIS VEGETASI MANGROVE DI EKOSISTEM MANGROVE DESA TAPIAN NAULI I KECAMATAN TAPIAN NAULI KABUPATEN TAPANULI TENGAH PROVINSI SUMATERA UTARA Hotden P. Simamora; Khairijon '; Mayta Novaliza Isda
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Mangrove is vegetation that grows at the low tide area and the coastal region. A vegetation research analysis of mangrove had been done in Tapian Nauli I Village, Tapanuli, North Sumatra Province from April to May 2013. The aim of this study was to analyze the vegetation of mangrove forest. The method used in this study was a survey method, using a nested sampling. The parameters observed in this research were species compositions and important value index. The result showed that six mangrove species were identified. The highest density for seedling (26%) and for sapling stage (23%) were found at Avicennia marina, while for tree stage (30%)was found at Rhizophora apiculata. The highest dominance for seedling and sapling stage were found at Aveicennia marina and Rhizophora apiculata(24%), while for tree was found at Rhizophora apiculata (28%). The highest frequency for seedling (28,47% ) and sapling (27,47%) were found at R. marina while for tree stage was found at Rhizophora apiculata (28.46%). The highest importance value index at seedling (78,47%) and sapling stage (74,27%) were found at Aveicennia marina while for the tree stage was found at Rhizophora apiculata (86.46%).
INDUKSI TUNAS DARI EKSPLAN BIJI MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS SECARA IN VITRO DENGAN PERLAKUAN BAP (Benzylaminopurine) PADA MEDIUM MS Ria Yuni Rahmawati; Mayta Novaliza Isda; Siti Fatonah
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Mangosteen (Garcinia mangostana L.) from Bengkalis island has many superiorities such as growing in the marshes and tolerant to acid soil (peat soil). In vitro culture is an alternative way to get large number and uniform mangosteen seedling. The purpose of this research was to determine the best concentration of BAP (Benzylaminopurine) in inducing mangosteen’s shoots from Bengkalis. This research used Randomized Complete block Design (RAK) with 5 concentrations of BAP (0; 1; 3; 5; 7 mg/l) with 5 replications. The result of this research showed the fastest shoot forming time and highest number of shoots present in a concentration of 5 mg / l BAP at 13 DAP and 2 shoots per explant.
PENGUJIAN KUALITAS PIGMEN ANTOSIANIN PADA BUNGA SENDUDUK(Melastoma malabathricum L.) DENGAN PENAMBAHAN PELARUT ORGANIK DAN ASAM YANG BERBEDA Indang Julita; Mayta Novaliza Isda; Wahyu Lestari
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Anthocyanin is red, purple and blue pigment that is commonly found in plants and can be used as a natural dye. One of plant species that can be extracted as natural dye source and contains anthocyanin is senduduk (Melastoma malabathricum L.). The objective of this study was to compare the effectivenes of various solvents and organic acids in extraction process of antocyanin pigment from senduduk flower. The results showed that the color of anthocyanin extracts that produce the strongest color was found in 3% ethanol and 1% tartaric acid treatment, while the weakest color was found in 3% ethanol treatment. Low pH was produced in 3% ethanol + 1% tartaric acid, while high pH was produced in 3% ethanol treatment.
POTENSI EKSTRAK Peperomia pellucida (L.) TERHADAP PENGHAMBATAN PERKECAMBAHAN DAN PERTUMBUHAN Mikania micrantha (K.) Kurnia Sari; Siti Fatonah; Mayta Novaliza Isda
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Weed is one of the limiting factors of crop production. Some of weed controls use synthetic and organic herbicides. Organic herbicides are used as the environmentaly friendly weed control. One of potential plants as organic herbicides is Peperomia pellucida that contains allelopathic compound and essensial oil. The purposes of this research were to determine the effect of P. pellucida extract and the most effective and efficient extract concentration in inhibiting the germination and growth of Mikania micrantha. This research used Randomized Complete Design using four treatments, i. e. control, 7,5%, 15% and 30% of P. pellucida extracts. The collected data was analyzed using Analysis of Variance (ANOVA) and followed by Duncan’s Multiple Range Test (DMRT) if the result was significantly different. The result showed the decrease of the germination and growth of M. micrantha weed. The most effective and efficient extract was 7,5% P. pellucida extract.
Co-Authors . Mansyurdin Akrom, Akrom Anak Agung Gede Sugianthara Angriawan Markal Anisa Sholihatul Muawanah Asih Rahayu Ajeng Agesti Awit Tatas Asih Susanti Delita Zul Djoko Santoso Fitmawati Fitmawati Herman Herman Hotden P. Simamora Indah Wijayanti Indang Julita Isnaini Isnaini Jamhari Jamhari Khairijon ' Kurnia Sari Maya Sari Melda Jannatul Salsabilla Musliar Kasim Nery Sofiyanti Nurul Hidayati Purwoko, Agus Rahmahayu ' Retno Ayu Wulandari Reynhard ' Ria Yuni Rahmawati Rodesia Mustika Roza Sari Malinda Sujarwati Sujarwati Syafroni Pranata Tetty CHAIDAMSARI Tri Septiani