Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

3.078

P-Index

This Author published in this journals

All Journal Jurnal Ilmu Lingkungan Indonesian Journal of Forestry Research Jurnal Sumberdaya Alam dan Lingkungan Natural B BERITA BIOLOGI Jurnal Penelitian Pertanian Terapan Jurnal Teknologi & Industri Hasil Pertanian JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Riset Akuakultur Bioma : Jurnal Biologi dan Pembelajaran Biologi Indonesian Journal of Chemical Analysis (IJCA) Jurnal Kreativitas PKM AGROVITAL : Jurnal Ilmu Pertanian Jurnal Ilmiah Farmasi Farmasyifa Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal ABDINUS : Jurnal Pengabdian Nusantara Buguh: Jurnal Pengabdian Kepada Masyarakat Sriwijaya Bioscientia Prosiding Konferensi Nasional PKM-CSR NEAR: Jurnal Pengabdian kepada Masyarakat Gunung Djati Conference Series JURNAL BIOLOGI PAPUA Jurnal Kedokteran Hewan AMMA : Jurnal Pengabdian Masyarakat Indonesian Journal of Forestry Research

Sumardi, Sumardi

FMIPA Universitas Lampung

Author-ID : 1099855

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary Other

Published : 28 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3

PROBIOTIC CANDIDATE PROTEOLYTIC Bacillus sp. COLLECTED FROM MANGROVE OF MARGASARI, LAMPUNG Sumardi Sumardi; Komang Rima; Salman Farisi; Endang Linirin Widiastuti
Indonesian Journal of Forestry Research Vol 8, No 1 (2021): Indonesian Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2021.8.1.73-82

Intensive shrimp culture has encountered many problems, such as declining water quality through disease caused by pathogenic microbes, which affected mortality. This study aimed to determine any potential probiotic from Bacillus sp. collected from mangrove in East Lampung, which could be used to improve the cultured shrimps' proteolytic and probiotic activity. This is a descriptive research with sampling and data collection of bacteria from many samples of mangrove. Result shows 128 isolates Bacillus from which then it has arrived at five potential probiotic Bacillus sp. The study five Bacillus sp. has been isolated with potential properties for probiotic (KPP212, IP121, UJ131, UJ132, SB141). Each isolate has characteristics with proteolytic property, growth in a wide range of pH 4–10 and osmotic stress (0–6% NaCl), non-pathogenic, ability for glucose fermentation, non-motile, and has negative catalase activity. The five potential Bacillus sp. can be used as probiotics for shrimp farming.

Lipolytic-screening of Bacillus genera as Biocontrol candidate In Coffee Plantation Ema Ervina; Cristina Nugroho Ekowati; Sumardi Sumardi; Emantis Rosa
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.12

Problems in decreasing coffee production one of them caused by plant pests attack. Countermeasures using pesticides and disinfectants are not effective because they have long effects and ruining the environment also pest resistance. It is necessary to prevent a more environmentally friendly way by utilizing a natural enemy in the form of a microorganism, the genus Bacillus. Lipase can hydrolyze lipids so that it can be used to degrade lipid substrates that compile the body structure of pests and diseases. This research aims to detect the lipolytic activity of Bacillus isolates from coffee plantations. The results of this research obtained 3 isolates of Bacillus namely T1, T2, and T3 which have differences in cell configuration and variations in the location of endospores. Furthermore, Bacillus isolates were detected lipolytic activity by growing isolates on lipase selective medium. Isolates that have the largest lipolytic activity are T2 isolate codes with an average index of 6.01 and the lowest lipolytic activity, namely, isolate T1 codes with an average index of 4.58.

The Effect of Taurine on Superoxide Dismutase Enzyme Activity, Malondialdehyde And Histology In Liver Mice (Mus Musculus) Male-Induced Herbicide Glyphosate Elfa Verda Puspita; Gregorius Nugroho Susanto; Sumardi Sumardi; Endang Linirin Widiastuti
Natural B, Journal of Health and Environmental Sciences Vol 3, No 3 (2016)
Publisher : Natural B, Journal of Health and Environmental Sciences

Glyphosate herbicide widely used by farmers in Indonesia. As a result of exposure to herbicides, the production of free radicals or ROS exceeds the body's defense system called oxidative stress. Therefore, the activity of antioxidant enzymes in the body decline and thus can cause tissue damage and certain organ dysfunction. ROS can be suppressed by administration of antioxidants. One source of the antioxidant is taurine. Taurine has a protective effect against oxidation and free radical capture in a variety of cells and tissues against toxic oxidant component. This study aims to determine the effect of taurine in the liver of mice induced herbicide glyphosate on the activity of the enzyme superoxide dismutase (SOD), malondialdehida (MDA), changes in morphology and structure of liver histology. The research used completely randomized design (CRD). Mice were divided into three treatment groups such as, control group (K0), a group of glyphosate (K1) at a dose of 25 mg /kgBW/day orally for 25 days, and a group of taurine+glyphosate (K2) at a dose of taurine 7800 mg/kgBW/day + glyphosate dose 25 mg/kgBW/day orally for 25 days. Each treatment consisted of 8 mice as replication. Results of analysis with one ways ANOVA (P<0.05) followed by the Tukey test showed taurine dose of 7800 mg/kgBW/day can reduce liver cells of male mice that were treated by the herbicide glyphosate but it not be able to increase the levels of SOD and to decrease MDA levels.

Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp Sumardi Sumardi; Rochmah Agustrina; Bambang Irawan; Indah Selfiana
Jurnal Ilmu Lingkungan Vol 16, No 2 (2018): Oktober 2018
Publisher : School of Postgraduate Studies, Diponegoro Univer

ABSTRAKTujuan penelitian ini adalah mengetahui pengaruh paparan medan magnet 0.2 mT pada ion logam Fe dan Zn dalam media pertumbuhan terhadap produksi protease Bacillus sp. Ion logam yang digunakan yaitu Fe dan Zn dalam bentuk garam FeCl3 dan ZnCl2. Hasil penelitian menunjukkan bahwa ion logam Fe (0.01% b/v) yang terpapar medan magnet mempunyai nilai Indeks Proteolitik 3,36 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,74 dengan lama inkubasi 18 jam. Sedang ion logam Fe (0.01% b/v) yang tidak terpapar medan magnet mempunyai nilai Indeks Proteolitik 1,79 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,12 dengan lama inkubasi 18 jam. Sedangkan pada ion logam Zn (0.005% b/v) yang terpapar medan magnet dan tidak terpapar medan magnet menyebabkan aktivitas Bacillus mati. Larutan ion logam Fe 0,01% dalam media cair menghasilkan produksi protease lebih baik (0,06 U/ml) dibandingkan dengan produksi protease tanpa dipemapar medan magnet (0,00 U/ml).Kata kunci: Bacillus sp., paparan medan magnet, indeks proteolitik.ABSTRACTThe purpose of this study was to determine the effect of metal ions Fe and Zn were exposed to a magnetic field of 0.2 mT to the production of proteases in Bacillus sp. The metal ions used are Fe and Zn in the form of FeCl3 and ZnCl2 salts. The results showed that the culture medium containing metal ions Fe (a 0.01% w/v) were exposed by the magnetic field has a value of proteolytic index of 3.36 with 10 hour long incubation, and proteolysis index of 2.74 with 18 hour incubation time. Culture medium containing metal ions Fe (a 0.01% w/v) which are not being exposed to the magnetic field has a value of proteolytic index of 1.79 with 10-hour long incubation, and proteolysis index of 2.12 with 18 hour incubation time. While the culture medium containing metal ions Zn (0.005% w/v) either exposed or not exposed to the magnetic field causes the activity of Bacillus sp die. The solution of 0.01% Fe metal ion in liquid medium resulted in better protease production (0.06 U/ml) than production of proteases without exposure to magnetic fields (0.00 U/ml)Keywords: Bacillus sp., magnetic field, proteolytic index, and proteolysis indexSitasi: Sumardi, Agustrina R., Irawan B., dan I. Selfiana. (2018). Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp. Jurnal Ilmu Lingkungan, 16(2), 173-177,doi:10.14710/jil.16.2.173-177

PENGARUH PEMAPARAN MEDAN MAGNET 0,2 mT PADA MEDIA YANG MENGANDUNG LOGAM (Al, Pb, Cd, dan Cu) TERHADAP Bacillus sp DALAM MENGHASILKAN PROTEASE Sumardi Sumardi; Rochmah Agustrina; Bambang Irawan; Shofia Rodia
BERITA BIOLOGI Vol 19, No 1 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i1.3780

This study aimed to determine the effect of 0.2 mT magnetic field exposure on metals (Al, Pb, Cd and Cu) containing media, to proteolytic index of Bacillus sp., protease activity and cell morphology of Bacillus sp. This study consisted of three stages. The first stage was a proteolytic test on solid media containing milk. The second stage was the production of protease enzymes in liquid media. The third stage was Scanning Electron Microscopy (SEM) analysis on a culture treatment known as protease enzyme activity. The results showed that the largest proteolytic index on CuCl2 metal ion content of magnetic 0.2 mT magnetic field for 10 minutes increased the value of the largest proteolytic index was 4.33. While in the ion solution containing CdCl2, Bacillus sp. culture did not grow. In the production of enzymes in the liquid medium, the highest enzyme activity (0.140 U/ ml) was produced on a medium containing AlCl3 and exposed to magnetic fields. The SEM analysis also proved that the supplementation of AlCl3 increased cell length by 2.38 and 2.78 times longer than control without magnetic field and for magnet field, respectivelly.

Pengaruh Pemaparan Medan Magnet Pada Media Mandels Yang Dimodifikasi Terhadap Pertumbuhan Dan Aktivitas Enzim Selulase Bacillus Sp. wayan hernawati; sumardi sumardi; rochman agustrina; herman yulianto
Jurnal Penelitian Pertanian Terapan Vol 16 No 2 (2016)
Publisher : Politeknik Negeri Lampung.

Enzymes can undergo structural changes due to various environmental factor such as pH,temperature, electricity and magnetic fields. The purpose of this study was to investigate theeffect of magnetic field strength and duration of exposure to the modified of media Mandelsused as a culture medium of Bacillus sp. on the growth and activity of cellulase enzyme fromthe bacterium Bacillus sp. Factorial study group were prepared using the method of fullrandomized design (RKLT). The first factor is the strong magnetic field consists of 4 levels ofmagnetic field strength treatment: 0 mT; 0,1 mT; 0,2 mT; 0.3 mT. The second faktor is theprolonged exposure consisting of 4 levels prolonged exposure treatments 0, 10, 20, 30minutes. each unit treatment was repeated 3 times. The results showed that exposure tomagnetic fields on modified of media Mandels causes a decrease in the relative activity ofthe enzyme cellulase and relative growth of Bacillus sp.Keywords: cellulase enzyme, magnetic field, Mandels modification, Bacillus sp.

IMOBILISASI BAKTERI ASAM LAKTAT DENGAN MENGGUNAKAN ZEOLIT DAN NATRIUM ALGINAT Kinasih Cahyono; Sumardi Sumardi; Bambang Irawan; Sri Wahyuningsih; Endang Nurcahyani
Jurnal Ilmiah Farmasi Farmasyifa Vol 4, No 1 (2021)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jiff.v4i1.6372

Lactobacillus sp merupakan salah satu spesies yang sering digunakan sebagai probiotik, namun kelemahan dari bakteri tersebut adalah tidak toleran terhadap pH rendah (asam), di cairan empedu, serta pada suhu yang tinggi. Bakteri probiotik harus tetap hidup sejak mereka dikonsumsi hingga menetap di usus. Hal ini sulit karena bakteri harus melewati pH asam ekstrim di saluran pencernaan. Tujuan dalam penelitian ini adalah meningkatkan viabilitas BAL dalam kondisi simulasi asam lambung (pH=2) dan garam empedu (ox bile 0,5%) yang diimobiliasi dengan zeolite-natrium alginat. Enkapsulasi bakteri probiotik adalah alternatif yang memberikan perlindungan bagi sel-sel hidup yang berada pada kondisi yang merugikan. Berdasarkan hasil uraian di atas maka bertujuan untuk meningkatkan jumlah kelangsungan hidup bakteri Lactobacillus sp dalam kondisi simulasi asam lambung dan garam empedu bakteri probiotik dengan menggunakan imobilisasi natrium alginat-zeolit serta mengetahui gen penghasil antibiotik pada bakteri asam laktat. Metode yang digunakan dalam imobilisasi bakteri adalah ekstrusi. Berdasarkan hasil penelitian maka dapat disimpulkan bahwan Imobilisasi dengan penyalut natrium alginate-zeolit mampu meningkatkan viabilitas bakteri asam laktat. Dalam kondisi simulasi lambung dan garam empedu. Bakteri asam laktat (BAL) menghasilkan bakteriosin yang dapat berfungsi untuk menghambat bakteri Gram positif dan Gram negatif berasal dari gen penghasil antimikroba yaitu terdapat gen plantaricin, casseicin, acidocin, lactacin B, helviticin Kata Kunci : Imobilisasi, Natrium Alginat, Bakteri Asam Laktat, Zeolit, Gen Antimikroba

Evaluasi mutu susu segar dari peternak berbeda di provinsi Lampung sebagai bahan baku keju [The quality of fresh milk from different farmers in Lampung as a raw material for cheese] Neti Yuliana; Iyan Indrawan; Chandra Utami Wirawati; Sumardi Sumardi
Jurnal Teknologi & Industri Hasil Pertanian Vol 26, No 2 (2021): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtihp.v26i2.109-114

The objective of this study was to evaluate the quality of fresh cow’s milk from different farmers in Lampung Province as a raw material for cheese in view of the Indonesian National Standard SNI 31411 (2011) regulation. Twelve fresh milk samples from four different districts (Tanggamus, Metro, Gisting, and Pesawaran) were evaluated by estimation of total protein and fat, pH, alcohol test, total plate count, antibiotic residues, and sensory. The result of the study revealed that the samples had protein and fat ranged from 2.44 to 3.90 %, and from 3.48 to 4.73% (wet basis) respectively. The fresh milks had normal sensory and pH, negative of alcohol test, and free from antibiotic residues. Total microbes were detected in log 5-6 CFU/ml. Majority of the samples screened met the Indonesian National Standard and could be used as a raw material for cheese making. Keywords: cheese, fresh milk quality, Lampung province, SNI 31411,

AKTIVITAS DAN KARAKTERISASI ENZIM PROTEASE ISOLAT Bacillus sp. (UJ132) SECARA KUALITATIF DAN KUANTITATIF Sumardi Sumardi; Salman Farisi; Chiristina Nugroho Ekowati; Milsa Solva Diana
Jurnal Riset Akuakultur Vol 14, No 3 (2019): (September, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Bakteri penghasil enzim protease memiliki kemampuan untuk melakukan bioremediasi limbah protein. Penelitian ini dilakukan untuk mengetahui aktivitas dan karakterisasi enzim protease dari isolat Bacillus sp. (UJ132) yang diisolasi dari udang pasir (Metapenaeus affinis) di kawasan hutan mangrove Desa Margasari, Lampung Timur. Uji aktivitas enzim dilakukan secara kualitatif dan kuantitatif. Karakterisasi enzim meliputi penentuan suhu dan pH optimum, pengaruh ion logam, serta penentuan Km dan Vmaks. Dari hasil percobaan diketahui bahwa enzim protease dihasilkan pada waktu produksi optimum 18 jam dengan aktivitas protease sebesar 0,09 U/mL. Suhu optimum enzim ini yaitu pada suhu 50°C yang menghasilkan aktivitas sebesar 0,08 U/mL. Enzim protease ini mempunyai kondisi optimum pada pH 5 dengan nilai aktivitas 0,09 U/mL. Semua ion logam (Ca2+, Mn2+, Cu2+, Mg2+) berfungsi sebagai inhibitor kecuali ion Fe3+ yang berfungsi sebagai aktivator pada konsentrasi 1 mM dan 5 mM. EDTA dengan konsentrasi 1 mM dan 5 mM berfungsi sebagai inhibitor pada enzim protease isolat UJ132. Nilai Vmaks enzim protease 0,33 U/mL sedangkan Km senilai 4,59 mg/mL substrat, enzim ini mempunyai afinitas yang tinggi terhadap substrat.Shrimp farming produces protein wastes which mainly come from the remnants of given feed and excreta (feces) of shrimp. Bacteria known to possess protease enzymes have the ability to solve this protein waste problem in the shrimp farming industry. This study was conducted to determine the production and characterization of protease enzyme from Bacillus sp. (UJ132) isolated from the mangrove forest area of Margasari Village of Lampung Timur. The enzyme activity test was done qualitatively and quantitatively. The objectives of this study were to determine the optimum production of the enzymes and observe their characteristics, including determining the temperature and optimum pH, the effect of several metal ions, as well as Km and Vmax. The experimental results revealed that the protease enzyme had an optimum time of 18 hours of protease activity as much as 0.09 U/mL. The optimum temperature of this enzyme was 50°C which produced an activity of 0.08 U/mL. This protease enzyme has an optimum working condition at pH 5 with an activity value of 0.09 U/mL. All metal ions (Ca2+, Mn2+, Cu2+, Mg2+) acted as inhibitors except Fe3 + ions which acted as activators at concentrations of 1 mM and 5 mM. EDTA with a concentration of 1 mM and 5 mM served as an inhibitor of UJ132 isolate protease enzyme. The value of Vmax of the protease enzyme was 0.33 U/mL while Km was 4.59 mg/mL suggesting that this enzyme has a high affinity with the substrate.

Pengaruh Bakteri Fotosintetik Anoksigenik terhadap Pertumbuhan Padi (Oryza sativa l.) Inpari 34 pada Media Salin Sumardi Sumardi; Rochmah Agustrina; Bambang Irawan; Siti Mardiana
AGROVITAL : Jurnal Ilmu Pertanian Vol 7, No 1 (2022): AGROVITAL VOLUME 7, NOMOR 1, MEI 2022
Publisher : Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/agrovital.v7i1.2020

Tujuan penelitian ini adalah untuk mengetahui pengaruh isolat bakteri fotosintetik anoksigenik (BFA) terhadap pertumbuhan padi (Oryza sativa L.) Varietas Inpari 34. Isolat BFA yang digunakan adalah dari bagian supernatan dan pelet sel BFA pada sumber yang sama. Parameter yang diamati adalah daya kecambah, pertumbuhan kecambah dan pertumbuhan padi pada lumpur mangrove yang salin. Analisis data daya kecambah hanya diketahui berdasarkan persentase perkecambahan. Data pertumbuhan kecambah dan pertumbuhan tanamanan dianalisis dengan ANOVA pada α = 0,05. Perlakuan pelet sel BFA dan supernatan BFA menghasilkan daya kecambah 96–100%. Hasil ANOVA menunjukkan bahwa pemberian isolat BFA dalam bentuk pelet dan supernatan memberikan hasil pertumbuhan kecambah yang signifikan. Isolat BFA dari pelet isolat meningkatkan panjang kecambah, jumlah akar, panjang akar secara nyata. Sedangkan,BFA dari supernatan isolat tidak mempengaruhi pertumbuhan kecambah. Isolat yang menghasilkan pertumbuhan kecambah yang baik adalah B2BM, B, D, AS, dan L2, sedangkan isolat AM dan L1 tidak menunjukkan pertumbuhan kecambah yang baik. Respon pertumbuhan daun yang baik dihasilkan dari isolat B, AS, dan L2, sedangkan isolat B2DM menghasilkan daun yang sedikit. Adapun parameter berat segar dan berat kering tidak menunjukkan respon yang berbeda terhadap perlakuan isolat.

Co-Authors Achmad Arifiyanto Achmad Arifiyanto Agustrina, Rochmah Arie Khoiriyah Arya R. Syah Azahra Putri Najla Bambang Irawan Berliana Damayanti C. N. Ekowati Chandra Utami Wirawati Chiristina Nugroho Ekowati Chistina Nugroho Ekowati Christina Ekowati Christina Nugroho Ekowati Christina Nugroho Ekowati Cindy Lukyta Ratih Riyanto Cindy Lukyta Ratih Riyanto Cristina Nugroho Ekowati Della M. Andini Dwi Septiani Eko Prihadi Elfa Verda Puspita Ema Ervina Emantis Rosa Emantis Rosa Endang Linirin Widiastuti Endang Linirin Widiastuti Endang Nurcahyani Faradilla Syani G. Nugroho Susanto Gregorius N. Susanto Hardoko Insan Qudus Hendri Busman herman yulianto Herta M. Manullang Indah Selfiana Intan Okta Nabilla Iyan Indrawan Jamhari Jamhari Kinasih Cahyono Komang Rima Kusuma Handayani M. Kanedi Madi Hartono Meilyana Santa Maria Meishy Handerlin Putri Milsa Solva Diana Miranti Syafitri NETI YULIANA Nuri Oktavia Posman Manurung Primasari Pertiwi Ratna Oktaviani Rina Maryani Rizka Oktavia Rochmah Agustrina rochman agustrina Rr. Etty Puspitaningsih N.W. Rudy Situmeang Salman Farisi Shofia Rodia Siti Mardiana Sri Wahyuningsih Susanto, Gregorius Nugroho Sutyarso Sutyarso Tintin Kurtini Tundjung Tripeni Handayani Vanya Qatrunada Vidia Royanti wayan hernawati Widia Angraini

Title

Found 28 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 28 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 28 Documents
Search