Protein Extract

Comparative UV absorption spectra analysis was carried out on seeds and seedlings of six Vigna unguiculata (L) Walp
accessions. Optical density (OD) UV absorbance (A) value was taken from A200 to A960 wavelengths using
spectrophotometer. At 280 nm protein content in Vigna unguiculata (L) Walp was between 4.2-5.7 % in seedling and 6.6-
16.9 % in seed. Absorbance spectra between 200-960 nm UV wavelengths of protein extracts from Vigna unguiculata (L)
Walp seeds and seedlings were not the same in each of the six accessions analyzed. The difference in the seeds and
seedlings protein absorbance spectra could possibly be due to difference in their protein chemical composition and shape.
With this level of detail in the analysis, the multiwavelength UV approach has the potential to provide a sensitive biosensor
for the detection, identification and enumeration of various biomolecules.

Comparative UV absorption spectra analysis was carried out on seeds and seedlings of six Vigna unguiculata (L) Walp accessions. Optical density (OD) UV absorbance (A) value was taken from A200 to A960 wavelengths using spectrophotometer. At 280 nm protein content in Vigna unguiculata (L) Walp was between 4.2-5.7 % in seedling and 6.6-16.9 % in seed. Absorbance spectra between 200-960 nm UV wavelengths of protein extracts from Vigna unguiculata (L) Walp seeds and seedlings were not the same in each of the six accessions analyzed. The difference in the seeds and seedlings protein absorbance spectra could possibly be due to difference in their protein chemical composition and shape. With this level of detail in the analysis, the multiwavelength UV approach has the potential to provide a sensitive biosensor for the detection, identification and enumeration of various biomolecules.