Proline Content

A pot experiment was conducted to evaluate the adverse effects of drought on different okra cultivars and to identify the most suitable okra cultivar for growing in drought conditions. Five okra cultivars namely Pusa Green, Clemson, Sabz Pari, Pusa Swani and Mehak Pari were subjected to three drought levels i.e., control (100% Field capacity),50% and 25% Field Capacity (FC). Physiological parameters like fresh and dry weight of plant and plant height were recorded along with biochemical attributes such as chlorophyll content (a, b, total) carotenoids, total protein, proline content, and Membrane stability index (MSI%). Results showed that drought significantly reduced all the studied parameters and at maximum drought (25% FC) lowest values of fresh weight (12.42g), dry weight (1.22g), plant height (7.86cm), chlorophyll a (9.02mg/g FW), chlorophyll b (18.69mg/g FW), total chlorophyll (27.71mg/g FW), carotenoids (11.80mg/g FW), total protein (2.73mg/g FW),whereas maximum Proline (21.3...

Drought stress influences seed germination and seedling growth of many plants. Seed coating is a technique of covering seeds with adhesive agents to improve seed performance and plant establishment and could be used to alleviate the negative effects of osmotic stress. In order to solve the problem of low germination and poor vigor of sweet corn under osmotic stress conditions (0, -0.3, -0.6 and -0.9 MPa), film coating with different concentrations of sodium alginate (NaAlg) (0, 1 and 2%) was performed. Results showed that osmotic stress reduced percentage and the rate of germination, seedling length, seedling vigor index and soluble protein content whereas increased the content of proline and soluble sugars. In all levels of osmotic stress, coating with NaAlg 1% improved germination and biochemical indices of sweet corn seeds compared to NaAlg 0 and 2%-and non-coated seeds. The highest percentage of germination (67 %) was related to osmotic potential of 0 MPa and the lowest germination percentage (36 %) was observed in the osmotic potential of -0.9 MPa. The highest (19.17 mg g -1 FW) and the lowest (6.68 mg g -1 FW) amount of soluble protein were obtained from NaAlg 1% at 0 MPa and NaAlg 0% at -0.9 MPa, respectively. The results suggested that seed coating with NaAlg 1% could serve as an appropriate treatment to increase the germination and early seedling growth of sweet corn under osmotic stress conditions.

Drought stress influences seed germination and seedling growth of many plants. Seed coating is a technique of covering seeds with adhesive agents to improve seed performance and plant establishment and could be used to alleviate the negative effects of osmotic stress. In order to solve the problem of low germination and poor vigor of sweet corn under osmotic stress conditions (0, -0.3, -0.6 and -0.9 MPa), film coating with different concentrations of sodium alginate (NaAlg) (0, 1 and 2%) was performed. Results showed that osmotic stress reduced percentage and the rate of germination, seedling length, seedling vigor index and soluble protein content whereas increased the content of proline and soluble sugars. In all levels of osmotic stress, coating with NaAlg 1% improved germination and biochemical indices of sweet corn seeds compared to NaAlg 0 and 2%and non-coated seeds. The highest percentage of germination (67 %) was related to osmotic potential of 0 MPa and the lowest germinati...

Wheat, the world's third important cereal, is cultivated in Pakistan both in irrigated and rainfed areas. One of the major constraints of wheat production in rainfed area is drought that needs to be addressed. Although many genotypes were released that were tolerant to drought stress by improving various physiological traits but there is a need to lessen the effects of drought to boost wheat productivity. The present study was designed to identify genotype(s) suitable under drought stress by analyzing various physiological traits such as relative water content, cell membrane stability, proline content and chlorophyll content under drought stress. The research material consisted of sixteen different wheat genotypes. The experiment was planted during winter 2013-14 following RCBD with three replications. Drought stress was induced by withholding water during heading and anthesis stages. High heritability, observed for relative water content, proline content and cell membrane stability, indicated that selection would be effective for these traits as they are heritable to next generation. Correlation analysis revealed that cell membrane stability and proline content were negatively correlated with relative water content while proline content was positively correlated with cell membrane stability. Yield was only positively correlated with chlorophyll content. Among tested wheat genotypes, Maxi-Pak, Chakwal 50 and WC 20 were identified as potential varieties that can be used in future wheat breeding programme for rainfed conditions.

The toxicity profile of coronatine (COR), a toxin produced by Pseudomonas bacteria using the Allium cepa test plant was determined with the help physiological analyzes including fresh weight, root number, root length and germination percentage; cytogenetic analyzes including chromosome aberration (CA), micronucleus frequency (MN), and mitotic index (MI); biochemical analyzes including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and proline (PR) accumulation; and microscopic analyzes including changes in root anatomical structure. A. cepa bulbs were divided into four groups as one control (C) and three application. Group C bulbs were kept in cuvettes containing tap water for 168 hours (7 days), while treatment group bulbs were kept in cuvettes containing 1, 5, and 10 μM COR solutions. COR administration caused a decrease in all physiological parameters examined, a rise in the CA and MN frequency, also a diminish in MI compared to C group. COR promoted CAs such as irregular mitosis, nuclear peak, exposure of chromosome scaffold, chromosome losses, unequal seperation of chromosome, vagrant chromosomes and chromatid bridges. In addition, the mentioned application caused a dosege-bound enhancement in free PR, CAT, SOD and MDA contents according to C group. Moreover, 10 μM COR, the highest application dose, caused quite significant damages such as epidermis cell deformations, micronucleus in epidermis/cortex, accumulation of various chemicals in the cortex layer, thickening of the cortex cell wall, flattened cell nuclei, necrosis and unclear transmission tissue in root anatomical structure of the bulbs. In summary, it was concluded that COR is a chemical with inhibitive impacts and the Allium cepa testing is a utility bioindicator for following these impacts.

A pot experiment was conducted to evaluate the adverse effects of drought on different okra cultivars and to identify the most suitable okra cultivar for growing in drought conditions. Five okra cultivars namely Pusa Green, Clemson, Sabz Pari, Pusa Swani and Mehak Pari were subjected to three drought levels i.e., control (100% Field capacity),50% and 25% Field Capacity (FC). Physiological parameters like fresh and dry weight of plant and plant height were recorded along with biochemical attributes such as chlorophyll content (a, b, total) carotenoids, total protein, proline content, and Membrane stability index (MSI%). Results showed that drought significantly reduced all the studied parameters and at maximum drought (25% FC) lowest values of fresh weight (12.42g), dry weight (1.22g), plant height (7.86cm), chlorophyll a (9.02mg/g FW), chlorophyll b (18.69mg/g FW), total chlorophyll (27.71mg/g FW), carotenoids (11.80mg/g FW), total protein (2.73mg/g FW),whereas maximum Proline (21.3...

Withania somnifera (L.) Dunal, commonly called Indian Ginseng (Ashwagandha), is an important medicinal plant having a number of chemotypes which vary in their regenerative potentiality. In the present study, regenerative potentiality of four chemotypes of W. somnifera was compared using leaf explants. The study correlated that the chemotype (NIMTLI-130) with high total phenolic content (TPC), high H 2 O 2 concentration and low proline content was less regenerative, whereas, the chemotype (NIMTLI-101) with lesser TPC, lower H 2 O 2 concentration and high proline content was more regenerative. NIMTLI-130 showed higher SOD, GPX and APX activity compared to NIMTLI-101, but less CAT activity as well as low proline content and a weak detoxification effect resulting in lower regeneration. The study revealed that the difference in in vitro shoot organogenesis of different chemotypes largely depends on the antioxidant protection system of the plant involving non-enzymatic compounds and activ...

Twenty-six maize landraces were tested in order to evaluate maize seedling performance as an index for drought tolerance in adult plants. Samples were subjected to polyethylene glycol-induced osmotic stress at the early seedling stage. Grain yield was obtained in field experiments under well-watered (OC) and a combination of drought and high plant density (HD) conditions. Osmotic stress caused a reduction in seedling growth (length, fresh and dry weight), and increase in the shoot and in particular the root proline contents in the majority of landraces, and variations in root peroxidase (POD) activity. Genotypes displaying more pronounced root growth reduction and higher proline contents exhibited decreased POD activity under osmotic stress. Direct positive correlations between the proline content and growth inhibition, and between the proline and soluble protein content were established. Correlations between the changes in POD activity and growth parameters were significant and pos...

Low temperature storage is the most effective technology for keeping quality and extending the postharvest life of fresh horticultural produce. However, horticultural produce of tropical and subtropical in origin such as marula fruit are susceptible to chilling injury (CI) when stored at temperatures below their critical minimum temperatures. Therefore, low temperature storage alone is not ideal for produce of tropical and subtropical in origin. The aim of this research was to elucidate the influence of modified atmosphere packaging (MAP) on CI of marula fruits. Storage temperature below 12ºC significantly (P < 0.05) increased CI incidence and severity, and proline content of marula fruit. Marula fruit in MAP had significantly (P < 0.05) lower electrolyte leakage than fruit stored in Air. The results further showed that marula fruit stored at 12?C in MAP had significantly longer shelf-life of 21 days than fruits in Air stored at various temperatures which had a shorter shelf l...

Low temperature storage is the most effective technology for keeping quality and extending the postharvest life of fresh horticultural produce. However, horticultural produce of tropical and subtropical in origin such as marula fruit are susceptible to chilling injury (CI) when stored at temperatures below their critical minimum temperatures. Therefore, low temperature storage alone is not ideal for produce of tropical and subtropical in origin. The aim of this research was to elucidate the influence of modified atmosphere packaging (MAP) on CI of marula fruits. Storage temperature below 12ºC significantly (P < 0.05) increased CI incidence and severity, and proline content of marula fruit. Marula fruit in MAP had significantly (P < 0.05) lower electrolyte leakage than fruit stored in Air. The results further showed that marula fruit stored at 12?C in MAP had significantly longer shelf-life of 21 days than fruits in Air stored at various temperatures which had a shorter shelf l...

Low temperature storage is the most effective technology for keeping quality and extending the postharvest life of fresh horticultural produce. However, horticultural produce of tropical and subtropical in origin such as marula fruit are susceptible to chilling injury (CI) when stored at temperatures below their critical minimum temperatures. Therefore, low temperature storage alone is not ideal for produce of tropical and subtropical in origin. The aim of this research was to elucidate the influence of modified atmosphere packaging (MAP) on CI of marula fruits. Storage temperature below 12ºC significantly (P < 0.05) increased CI incidence and severity, and proline content of marula fruit. Marula fruit in MAP had significantly (P < 0.05) lower electrolyte leakage than fruit stored in Air. The results further showed that marula fruit stored at 12?C in MAP had significantly longer shelf-life of 21 days than fruits in Air stored at various temperatures which had a shorter shelf l...

Wheat, the world's third important cereal, is cultivated in Pakistan both in irrigated and rainfed areas. One of the major constraints of wheat production in rainfed area is drought that needs to be addressed. Although many genotypes were released that were tolerant to drought stress by improving various physiological traits but there is a need to lessen the effects of drought to boost wheat productivity. The present study was designed to identify genotype(s) suitable under drought stress by analyzing various physiological traits such as relative water content, cell membrane stability, proline content and chlorophyll content under drought stress. The research material consisted of sixteen different wheat genotypes. The experiment was planted during winter 2013-14 following RCBD with three replications. Drought stress was induced by withholding water during heading and anthesis stages. High heritability, observed for relative water content, proline content and cell membrane stability, indicated that selection would be effective for these traits as they are heritable to next generation. Correlation analysis revealed that cell membrane stability and proline Original Research Article

A pot experiment was conducted to evaluate the adverse effects of drought on different okra cultivars and to identify the most suitable okra cultivar for growing in drought conditions. Five okra cultivars namely Pusa Green, Clemson, Sabz Pari, Pusa Swani and Mehak Pari were subjected to three drought levels i.e., control (100% Field capacity),50% and 25% Field Capacity (FC). Physiological parameters like fresh and dry weight of plant and plant height were recorded along with biochemical attributes such as chlorophyll content (a, b, total) carotenoids, total protein, proline content, and Membrane stability index (MSI%). Results showed that drought significantly reduced all the studied parameters and at maximum drought (25% FC) lowest values of fresh weight (12.42g), dry weight (1.22g), plant height (7.86cm), chlorophyll a (9.02mg/g FW), chlorophyll b (18.69mg/g FW), total chlorophyll (27.71mg/g FW), carotenoids (11.80mg/g FW), total protein (2.73mg/g FW),whereas maximum Proline (21.3...

Postharvest deterioration can result in qualitative and quantitative changes in the marketability of horticultural commodities, as well as considerable economic loss to the industry. Low temperature and controlled atmosphere conditions (low O2 and elevated CO2) are extensively employed to prolong the postharvest life of these commodities. Nevertheless, they may suffer from chilling injury and other physiological disorders, as well as excessive water loss and bacterial/fungal decay. Research on the postharvest physiological, biochemical, and molecular responses of horticultural commodities indicates that low temperature/controlled atmosphere storage is associated with the promotion of γ-aminobutyrate (GABA) pathway activity, with or without the accumulation of GABA, delaying senescence, preserving quality and ameliorating chilling injury. Regardless of whether apple fruits are stored under low temperature/controlled atmosphere conditions or room temperature, elevated endogenous GABA ...

Background Assessment of seed germination is an essential task for seed researchers to measure the quality and performance of seeds. Usually, seed assessments are done manually, which is a cumbersome, time consuming and error-prone process. Classical image analyses methods are not well suited for large-scale germination experiments, because they often rely on manual adjustments of color-based thresholds. We here propose a machine learning approach using modern artificial neural networks with region proposals for accurate seed germination detection and high-throughput seed germination experiments. Results We generated labeled imaging data of the germination process of more than 2400 seeds for three different crops, Zea mays (maize), Secale cereale (rye) and Pennisetum glaucum (pearl millet), with a total of more than 23,000 images. Different state-of-the-art convolutional neural network (CNN) architectures with region proposals have been trained using transfer learning to automatical...

Five grazing strategies were compared: grazing by ewes only and mixed grazing (ewes + heifers), in mixed grazing heifers received no supplement or one of three supplements. Ruminal undegradable protein (RUP) varied among supplement: 4.25, 9.11 and 9.13%, the supplement with the highest RUP included protected methionine. All supplements were 21% crude protein and 3.0 Mcal/kg of metabolizable energy. Grazing was on kikuyu, ratio was 30 kg of ewe per 100 kg of heifer in the mixed grazing treatments. Experimental grazing lasted 84 days. Variables measured were residual forage, proline content in residual forage and liveweight gains per animal and per hectare. Experimental design was a completely random with two replicates. Residual forage was the highest (P\u3c 0.05) in mixed grazing and RUP in supplement was above 4.25%. The highest (P\u3c 0.05) proline content was found in grazing by ewes only, 52% above the proline content found in mixed grazing without supplement, when heifers recei...

Uma das aplicações das técnicas da cultura de tecidos no melhoramento é a identificação de linhas de células que apresentam tolerância à salinidade. Vários autores obtiveram linhas de células tolerantes ao estresse salino; e estudo de mecanismos bioquímicos da tolerância a sais em plantas tem demonstrado altas correlações entre estes e o acúmulo de macromoléculas em tecido de plantas superiores. Para verificar essas correlações em feijão (Phaseolus vulgaris cv IAC carioca), calos oriundos de eixos embrionários foram cultivados em meio sólido, suplementado com NaCl nas concentrações de 0 a 60 mM. Após 13 dias de incubação, os calos foram coletados e analisados quanto ao crescimento relativo, teor de proteínas, teor de prolina e atividade da peroxidase. Os parâmetros analisados mostraram decréscimo no crescimento relativo e no de proteínas em resposta ao NaCl. Paralelamente, observou-se aumento significativo no conteúdo de prolina e atividade da enzima peroxidase.

Salinity is one of the environmental factors that has a critical influence on the germination of seeds and plant establishment. Salinity affects imbibition, germination and root elongation. However, the way in which NaCl exerts its influence on these vital processes, whether it is through an osmotic effect or a specific ion toxicity, is still not resolved. The objective of this study was to compare the salinity tolerance of two varieties of red clover (Select 2 and Rotrif) and two varietis of alfalfa (Mihaela and Mădălina). In our study we used five salinity treatments 0 (control) (C1), 50 (C1), 100 (C2), 150 (C3) and 300mM NaCl (C4) on normal seeds and seeds inoculated with rhizobia. 100 seeds of each variety used in the study were grown in germinating dishes on filter papers with four replications. The results showed that by increasing of the salinity level, germinated seeds number, root, shoot and seedling length, mean daily germination (MDG) and seedling length vigor index (SLVI) decreased, while daily germination speed (DGS) increased in all studied varieties. Mean germination time increased with increasing NaCl level. As the salt concentration increased, germination percentage decreased in all varieties, moreover, seeds could not germinate in 300 mM NaCl, but the seeds who were bacterized germinated in a very small percentage, and the germs were abnormal.

Low temperature storage is the most effective technology for keeping quality and extending the postharvest life of fresh horticultural produce. However, horticultural produce of tropical and subtropical in origin such as marula fruit are susceptible to chilling injury (CI) when stored at temperatures below their critical minimum temperatures. Therefore, low temperature storage alone is not ideal for produce of tropical and subtropical in origin. The aim of this research was to elucidate the influence of modified atmosphere packaging (MAP) on CI of marula fruits. Storage temperature below 12ºC significantly (P < 0.05) increased CI incidence and severity, and proline content of marula fruit. Marula fruit in MAP had significantly (P < 0.05) lower electrolyte leakage than fruit stored in Air. The results further showed that marula fruit stored at 12˚C in MAP had significantly longer shelf-life of 21 days than fruits in Air stored at various temperatures which had a shorter shelf life. It was concluded that marula fruits be stored in MAP at 12°C plus 90-95% RH to alleviate CI incidence and severity and maintain fruit quality and extend shelf-life.