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Laser Fluorescence

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lightbulbAbout this topic
Laser fluorescence is a spectroscopic technique that utilizes laser light to excite molecules, causing them to emit light at characteristic wavelengths. This emitted light, or fluorescence, is analyzed to provide information about the molecular structure, concentration, and dynamics of the sample, making it valuable in various scientific and industrial applications.
lightbulbAbout this topic
Laser fluorescence is a spectroscopic technique that utilizes laser light to excite molecules, causing them to emit light at characteristic wavelengths. This emitted light, or fluorescence, is analyzed to provide information about the molecular structure, concentration, and dynamics of the sample, making it valuable in various scientific and industrial applications.

Key research themes

1. How can laser-induced fluorescence be applied for biomedical tissue diagnosis, particularly in distinguishing normal versus pathological or cancerous tissues?

This theme focuses on leveraging laser-induced fluorescence (LIF) and laser-stimulated fluorescence techniques for improving diagnostic accuracy in biomedical contexts, especially for cancer detection. It explores spectral characteristics, spectral shifts, fluorophore interactions, and fluorescence lifetime imaging to differentiate normal tissue from malignant or premalignant lesions. Such approaches are critical as they enable non-invasive, sensitive early detection methods, which are crucial for timely treatment and better clinical outcomes.

Key finding: Demonstrated that laser-induced fluorescence spectra can reliably distinguish adenomatous (premalignant) colorectal polyps from normal and hyperplastic tissue with high sensitivity and specificity using an optical... Read more
Key finding: Presented laser-stimulated fluorescence (LSF) as a method yielding higher signal-to-noise than standard UV light, enabling fluorescence detection in specimens previously non-fluorescent under UV excitation. This method was... Read more
Key finding: Used spectral shift fluorescence microscopy with rhodamine 6G staining to differentiate melanoma, basal cell carcinoma, and squamous cell carcinoma from normal and benign tissues by quantifying specific shifts in fluorescence... Read more
Key finding: Found that autofluorescence intensities from typical endogenous fluorophores (e.g., FAD, NAD(P)H, porphyrins) are substantially reduced in squamous carcinoma cells compared to normal keratinocytes across multiple excitation... Read more

2. In what ways are advanced laser-induced fluorescence techniques and instrumentation advancing quantitative imaging and spectroscopy in fluids and multiphase flows?

This research area centers on improving spatially and temporally resolved measurements of chemical species concentrations, heat/mass transfer, and flow properties in fluid dynamics contexts using laser-induced fluorescence (LIF), planar LIF (PLIF), and time-resolved fluorescence imaging. It is critical for understanding complex interfacial phenomena, turbulence modulation by dispersed phases like bubbles, thin liquid film dynamics, and microfluidic processes, which have broad applications in engineering, environmental sciences, and biomedical fields.

Key finding: Reviewed and demonstrated the utility of planar LIF (PLIF) for quantitatively imaging spatial and temporal distributions of species and temperature in multi-phase flows including thin liquid films, bubbly flows, and sprays.... Read more
Key finding: Developed a multi-physics simulation model that predicts spatial and temporal fluorescence profiles of two-photon-excited diffusible fluorophores subjected to photobleaching and diffusion. Revealed that photobleaching leads... Read more
Key finding: Presented a confocal macroscopic fluorescence imaging system capable of simultaneously recording fluorescence decay profiles in 16 spectral channels and rapidly multiplexing excitation wavelengths. This system suppresses... Read more
Key finding: Reported the observation of anti-Stokes laser-induced fluorescence (ASF) in rhodamine B dye microdroplets suspended in low-thermal conductivity fluorocarbon oils within microfluidic channels. Demonstrated that localized... Read more

3. How can laser-induced fluorescence be utilized for remote sensing and environmental monitoring of soils, rocks, and ocean pollution?

This theme highlights efforts to employ laser-induced fluorescence spectroscopy, often combined with LIDAR techniques, to remotely identify and quantify pollutants, organic matter, and minerals in soils, rocks, and aquatic environments. Advances in portable instrumentation and spectral data analysis enable express diagnostics and pollution monitoring, which are vital for environmental management, ecological studies, and planetary exploration.

Key finding: Developed a compact nanosecond LIDAR system with tunable laser excitation (355, 532, 1064 nm) achieving remote LIF spectral measurements of Brazilian soils and rocks from mobile platforms. Created spectral databases and... Read more
Key finding: Calibrated laser-induced fluorescence spectral signatures for various marine oil products in dissolved and slick states, establishing detection limits for typical heavy and light marine fuels down to ppm levels. Used... Read more
Key finding: Demonstrated standoff (∼10 m) analysis of laser-produced plasmas by pumping aluminum atomic transitions with a cw tunable laser to induce LIF, achieving enhanced spectral resolution and signal persistence relative to... Read more
Key finding: Evaluated the efficacy of new powerful handheld UV lasers combined with protective eyewear to extend the detection range (up to fivefold) of fluorescent-marked organisms such as insects in field conditions. Compared with UV... Read more

All papers in Laser Fluorescence

Aim and purpose: The knowledge of health professionals about the potential risks of using ionizing radiation plays an important role in protecting themselves and patients. The use of mobile radiography units poses several threats to... more
Background: There has been an increased incidence of dental caries in children due to recent changes in lifestyle. More sensitive and reproducible diagnostic tools for precise caries detection in children are needed because of the complex... more
Minimal Intervention Dentistry (MID), a concept that aims to minimize the interventional procedures applied on teeth affected by dental caries, emphasizes the importance of prevention and detection of lesions in early stage. The white... more
Background: The diagnosis of hidden caries extending deeply into dentine with intact enamel is based on digital radiograph (RVG) and is considered a difficult procedure, which needs more analysis of the affected teeth. Aim: The purpose of... more
The vast and persistent Deepwater Horizon (DWH) spill challenged response capabilities, which required accurate, quantitative oil assessment at synoptic and operational scales. Although experienced observers are a spill response's... more
The aim of this in vivo study was to test the diagnostic accuracy of a pen-type laser fluorescence (LFpen) device in detecting approximal caries lesions, in posterior permanent teeth, at the cavitation and non-cavitation thresholds, and... more
Coronaviruses are positive singlestranded RNA viruses which are spread and transmitted to both humans and animals all around the world. The virus that causes COVID-19 disease is in a family of viruses that is called Coronaviridae. Most... more
Hypertensive patients deserve special attention, due to possible complications which may occur during invasive dental care. This manuscript has aimed at reviewing the literature regarding the following: a safe dental approach to... more
Indigenous peoples’ participation in public policy and planning is ascribed in numerous international and national legal instruments as essential to the realisation of their self-determination. This study examines how the Akwé: Kon... more
Coronaviruses are positive singlestranded RNA viruses which are spread and transmitted to both humans and animals all around the world. The virus that causes COVID-19 disease is in a family of viruses that is called Coronaviridae. Most... more
On December 31, 2019, the Wuhan Municipal Health Commission (Hubei Province, China) reports a cluster of pneumonia cases in the city. Subsequently, it is determined that they are caused by a new coronavirus. In response and after arduous... more
Hypertensive patients deserve special attention, due to possible complications which may occur during invasive dental care. This manuscript aimed at reviewing the literature as follows: a safe dental approach to hypertensive patients,... more
This study aimed to create a scale to measure post-traumatic stress disorder (PTSD) symptoms related to the COVID 19 crisis. Based on the available studies, the Davidson PTSD scale dimensions were used in this online survey. Accordingly,... more
Coronaviruses are positive singlestranded RNA viruses which are spread and transmitted to both humans and animals all around the world. The virus that causes COVID-19 disease is in a family of viruses that is called Coronaviridae. Most... more
Background: There has been an increased incidence of dental caries in children due to recent changes in lifestyle. More sensitive and reproducible diagnostic tools for precise caries detection in children are needed because of the complex... more
The aim of the research is to evidence the potential role of lactoferrin (LF) and heparin in coronavirus disease 2019 (COVID-19). Moreover, we discuss and underline the mechanisms involved in this possible association. Methods: PubMed and... more
An impact sprinkler with a newly designed nozzle-dispersion device and R33 sprinkler were introduced in this study. Comparative experiments and analyses on uniformity patterns of impinging water jet from both sprinklers were investigated... more
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