Haliburton, 2017 - Google Patents

Droplet Microfluidic Platforms for Single-Cell Sequencing

Haliburton, 2017

View PDF
Document ID
9040639402961065912
Author
Haliburton J
Publication year

External Links

Snippet

ABSTRACT DNA sequencing has defined an era of biological discovery by unveiling the genetic basis of biological processes. The completion of the human genome broadened understanding of the multiple scales of biological complexity from large scale structures of …
Continue reading at escholarship.org (PDF) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting

Similar Documents

Publication Publication Date Title
US12448643B2 (en) Sequencing of nucleic acids via barcoding in discrete entities
US11312990B2 (en) PCR-activated sorting (PAS)
US20230091562A1 (en) Methods and Devices for Analyzing Particles
US20220154248A1 (en) Combined multiple-displacement amplification and pcr in an emulsion microdroplet
US10427160B2 (en) Rapid and continuous analyte processing in droplet microfluidic devices
Streets et al. Chip in a lab: Microfluidics for next generation life science research
Zhang et al. Single-molecule DNA amplification and analysis using microfluidics
WO2018075577A1 (en) Methods of integrated microfluidic processing for preventing sample loss
Haliburton Droplet Microfluidic Platforms for Single-Cell Sequencing
Marcus Single mammalian cell gene expression analysis using microfluidics
Thompson Targeted Virus Detection and Enrichment Using Droplet Microfluidics
Caën Droplet microfluidics for cancer cell evolution
CN121992088A (en) Single-cell digital PCR based on digital microfluidics and its applications
Zec DEVELOPMENT OF MICROFLUIDIC PLATFORMS AS A TOOL FOR HIGH-THROUGHPUT BIOMARKER SCREENING
Kodzius Single-cell technologies in molecular marine studies