SU1589107A1 - Device for sterile samlpling to sealed container - Google Patents

Abstract

The invention relates to a sampling technique and can be used in installations for the microbiological synthesis of medical, microbiological, food and other industries, as well as in laboratory practice. The aim of the invention is to increase the reliability of known devices by improving the sterilization conditions. The device contains a sterilization chamber, in which the receiving container is placed with the possibility of axial movement, which is sealed with an elastic stopper. The chamber is divided into two cavities by means of a solid, hermetically installed spring-loaded piston with a central hole, in which the floor is hermetically fixed, and the needle pointed at both ends. One of the cavities of the sterilization chamber is in communication with the port for the entry of sterilizing steam, and the second with the pipe for its exit. When steam is supplied, the receptacle is sterilized by a stream penetrating the needle while simultaneously blowing it out. Sampling is carried out with axial movement of the receiving tank using a guide and piercing the plug with a needle. 1 il.

Description

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The invention relates to a sampling technique under the conditions of sterility and can be used in microbiological synthesis plants of microbiological, medical, food and other industries.

The aim of the invention is to increase the reliability of the device by improving the sterilization conditions.

The drawing shows the proposed device, a longitudinal section.

A device for sterile sampling in a sealed container consists of a sterilization chamber 1 in the form of a cylindrical body inside which there is a sealed pore, a Shen 2 with a fixedly fixed centrally hollow needle 3 pointed at both ends. The hermetic piston 2 is spring 4 pressed against the stop 5 for example, a spacer ring pressed into the wall of the cylindrical body of the sterilization chamber 1, the opposite end of the spring A rests on; the bottom of the old-fashioned chamber 1 serving as a flange base 6 cylindrical about the case, ending with a stupa 7, inside of which a hole 8 is made in the form of a truncated cone with its base facing the hollow needle 3. On the opposite end part of the cylindrical body, the cover 10 is installed through the grommet 9. The stem 12 passes through the O-ring I1 of the cover 0 associated with a package or a feed and return mechanism (not shown). Inside the sterilization chamber 1, the stem 2 is rigidly connected to the holder 13. Between the holder 13 and the gripping device 14 there is a receptacle 15 for samples of the type of penicillin vesicle. The gripping device 14 is rigidly connected to the guide 16 by the preloaded spring 17, which abuts the opposite end against the abutment 5. The stiffness of the spring 4 according to the working conditions of the Device is greater than the stiffness of the spring 17.

A hole 18 is provided for feeding steam into the device for sterilization in the flange base of the housing, and an opening 19 for dumping it on the opposite flange of the housing. To drain the steam condensate that accumulates in the cavity between the sealed piston 2 and the flange base 6, an opening 20 is provided sampling work 4: ka glushits.

The sampler through the elastic gasket 21 with the help of bolts or pin 22 is permanently and tightly attached to the bob of the cultivation apparatus 23, (fermenter, bioreactor, etc.),

The device works in a consistent manner.

After installing the sampling device on the apparatus from which sampling is to be performed, they are sterilized. For this purpose, sharp water vapor is fed into one of the fittings of the apparatus, and it is discharged through the other. At the same time, steam is supplied into the hole 18, which sterilizes the gasket 2 in the hole 8, the canal of the hollow needle 3 and the receiving tank 15. Steam is discharged through the hole 19. After the time specified by the regulations, the steam supply to the device 23 and

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sampler is terminated. After cooling, the apparatus is filled with a nutrient medium with the cultivated crop. From this point on, the device is ready for sampling in sterilized tank i

In order to take a sample by force on a handle (not shown), the scraper 12 together with the holder 13, the gripping device 14 and the receiving container 15 move to the right until the springs 17 and 4 are fully compressed. Since the stiffness of the spring 4 is greater than the spring 17, a hollow needle 3 first pierces the elastic stoppers of the receiving tank 15 (penicillin vesicle), and after the elastic strip 21, which prevents the sample from getting from the needle channel into the sterilization chamber 1. After the sample is collected, which enters the bottle under excessive pressure, the stem 12 moves m to the left is returned to the original position. In this case, due to the different stiffnesses of the springs 4 and 17, first the needle 3 under the action of the spring 4 comes out of the floor. seal (elastic strip 21), and then (after pressing the hermetic piston 2 to stop 5.) under the action of the spring 1 7 - from the receiving tank 15, which also prevents the sample from being thrown into the sterilization chamber 1.

In order to remove the receiving tank with the sample taken from the sterilization chamber, the cover 10 is broken and disconnected from the housing of the sterilization chamber 1. After that, the sample is released from the holder 13 and the gripping device 14 and transported to the consumer. In its place is installed in the holder of a similar capacity. It is then inserted into a gripping device, which has bends that abut against the inclined part of the balloon of the receiving container 15, which allows it to be moved to the right for sampling. In turn, the bends provide a passage for the head of the receiving tank and its removal from the gripping device.

After the receiving tank is installed in the holder 13 and the gripping device 14, the clamp 10 is attached to the flange portion of the cylindrical housing of the sterilization chamber 1 and the steam sterilization of the sampling device is performed, after which the sampling cycle is repeated.

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Editor A. Makovska

Compiled by S. Brazhnikov

Tehred L. Sardyukova Proofreader L. Patay

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Claims (1)

Claim A device for sterile sampling in a sealed container containing a sterilization chamber with nozzles for entering and exiting the sterilizing agent, a receiving container with a gripping device located inside the sterilization chamber, and a hollow needle pointed at both ends, installed with the possibility of axial movement, characterized in that , in order to increase the reliability of the device by improving the sterilization conditions ³ , the sterilization chamber is divided into two cavities by means of a continuous, hermetically sealed A fixed piston with a central bore spring-loaded relative to the bottom of the IQ a ring mounted with the possibility of interaction with the piston, and provided with a spring. relative to the spacer ring of the guide, rigidly connected to the gripping device of the receiving tank.