MX2016003617A - Metodo para producir molecula de enlace al antigeno usando fago auxiliar modificado. - Google Patents

Metodo para producir molecula de enlace al antigeno usando fago auxiliar modificado.

Info

Publication number
MX2016003617A
MX2016003617A MX2016003617A MX2016003617A MX2016003617A MX 2016003617 A MX2016003617 A MX 2016003617A MX 2016003617 A MX2016003617 A MX 2016003617A MX 2016003617 A MX2016003617 A MX 2016003617A MX 2016003617 A MX2016003617 A MX 2016003617A
Authority
MX
Mexico
Prior art keywords
binding molecule
helper phage
producing antigen
modified helper
polypeptide
Prior art date
Application number
MX2016003617A
Other languages
English (en)
Inventor
Shinya Ishii
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of MX2016003617A publication Critical patent/MX2016003617A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14111—Inoviridae
    • C12N2795/14131—Uses of virus other than therapeutic or vaccine, e.g. disinfectant
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14111—Inoviridae
    • C12N2795/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2795/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención proporciona un método para preparar un bacteriófago que muestra una molécula de enlace al antígeno, que comprende el paso de poner en contacto un fago auxiliar capaz de expresar un primer polipéptido con una bacteria capaz de expresar un segundo polipéptido, en donde el primer polipéptido y el segundo polipéptido se asocian uno con el otro para formar la molécula de enlace al antígeno.
MX2016003617A 2013-09-30 2014-09-30 Metodo para producir molecula de enlace al antigeno usando fago auxiliar modificado. MX2016003617A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2013203528 2013-09-30
PCT/JP2014/076001 WO2015046554A1 (ja) 2013-09-30 2014-09-30 改変されたヘルパーファージを用いて抗原結合分子を作製する方法

Publications (1)

Publication Number Publication Date
MX2016003617A true MX2016003617A (es) 2016-07-21

Family

ID=52743675

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016003617A MX2016003617A (es) 2013-09-30 2014-09-30 Metodo para producir molecula de enlace al antigeno usando fago auxiliar modificado.

Country Status (10)

Country Link
US (1) US10501737B2 (es)
EP (2) EP3037525B1 (es)
JP (2) JP6841596B2 (es)
KR (1) KR102282691B1 (es)
CN (1) CN105765063B (es)
BR (1) BR112016006502A2 (es)
CA (1) CA2922950A1 (es)
EA (1) EA201600303A1 (es)
MX (1) MX2016003617A (es)
WO (1) WO2015046554A1 (es)

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Also Published As

Publication number Publication date
JP7036878B2 (ja) 2022-03-15
CA2922950A1 (en) 2015-04-02
EP3037525A4 (en) 2017-05-03
EA201600303A1 (ru) 2016-11-30
US10501737B2 (en) 2019-12-10
EP3037525A1 (en) 2016-06-29
JP6841596B2 (ja) 2021-03-10
CN105765063B (zh) 2021-05-07
KR20160062050A (ko) 2016-06-01
EP3037525B1 (en) 2021-06-02
WO2015046554A1 (ja) 2015-04-02
JP2021019597A (ja) 2021-02-18
BR112016006502A2 (pt) 2017-09-19
JPWO2015046554A1 (ja) 2017-03-09
EP3940065A1 (en) 2022-01-19
US20160264960A1 (en) 2016-09-15
CN105765063A (zh) 2016-07-13
KR102282691B1 (ko) 2021-07-27

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