KR20250090450A - Method for manufacturing nanoparticle micelle and nanoemulsion cosmetic composition and manufacturing method for thereof, roller-type cosmetic container, and cosmetic product - Google Patents

Abstract

The present invention relates to a method for producing nanoparticle micelles having triple functionality of improving itchiness by strengthening the skin barrier and whitening, improving wrinkles, and blocking ultraviolet rays by producing functional peptide-ceramide micelles using a centrifugal microfluidic reactor, a nanoemulsion cosmetic composition thereof and a method for producing the same, and a roller-type cosmetic container and a cosmetic product.
In particular, the present invention efficiently produces a nanoemulsion cosmetic composition having excellent skin adhesion, formulation stability, and skin percutaneous absorption rate by stabilizing the composition into nanoparticle micelles by applying peptides and ceramides.
In addition, the present invention is a roller-type cosmetic product containing a functional cosmetic composition as a content, and can be usefully used as a triple-functional cosmetic product of a cosmetic product manufactured with a roller-type cosmetic container equipped with an eco-friendly container lid with a mirror function, which improves the formulation stability and percutaneous absorption rate of active ingredients, and has an anti-itching effect and a non-irritating product with a skin irritation index of 0.00 without skin irritation.

Description

Method for manufacturing nanoparticle micelle and nanoemulsion cosmetic composition and manufacturing method for thereof, roller-type cosmetic container, and cosmetic product}

The present invention relates to a method for producing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor, a nanoemulsion cosmetic composition thereof and a method for producing the same, and a roller-type cosmetic container and a cosmetic product.

In order to succeed in developing a multi-functional cosmetic composition with excellent competitiveness, it is urgently necessary to develop a formulation technology that can stably disperse various functional ingredients and a formulation technology that can preserve dispersion stability for a long time.

Research on delivering drugs or functional substances into living tissues plays an important role in the bio and medical fields, and securing related technologies is directly related to the competitiveness of the relevant industry, so research on delivering functional effective ingredients in various forms has been ongoing for a long time.

Among the methods proposed so far, methods using lipid nanoparticles such as liposomes and nanodisks such as Bicelle are known to have excellent biocompatibility and in vivo delivery efficiency, and many multinational companies and domestic and foreign research institutes are conducting technological development.

Liposomes can be used as protective and delivery vehicles that can protect or transport functional substances such as proteins and drugs by loading them inside and outside, and demand is increasing in various fields. Lipid-based nanodisks such as commercialized liposomes are manufactured based on processes such as mixing and stirring of large-scale solutions, but market expansion is limited due to difficulties in miniaturizing and homogenizing the synthesized liposomes.

Meanwhile, cosmetics are manufactured and used as solid, liquid, or gel-type cosmetics, and various cosmetic containers are manufactured according to the type of cosmetics to store them. In general, cosmetics are used in dedicated cream cosmetic containers that are opened and closed with a lid having a relatively wide opening, or in tube-shaped cosmetic containers. Tube-shaped containers are formed of various soft materials such as aluminum, laminate, and synthetic resin, and are widely used as containers for storing cosmetics, medicines, toothpaste, shampoo, detergents, etc. in the form of creams, by compressing the surface and discharging the cosmetic liquid through a relatively narrow injection port.

Cosmetics in the form of creams, such as sunscreens, are generally stored and used in tube-type cosmetics containers. These tube-type cosmetics containers are widely used as containers to effectively seal and store not only cosmetics, but also medicines in the form of creams, as well as various detergents such as toothpaste and shampoo. In the case of conventional cosmetic liquid application containers, liquid substances are discharged from the container and applied to the desired location using hands or a brush.

In addition, although tube-type cosmetic containers have the advantage of being able to effectively store contents and being convenient to use, there is a problem in that the overall volume decreases as much as the contents are discharged due to the characteristics of the tube itself, which inevitably leads to deformation of the appearance.

That is, since the shape of the tube gradually became distorted as the amount used increased, it could not maintain its original shape, so it did not look good and had structural problems, and there was also the problem of poor storage due to the deformed shape.

In addition, tube-type cosmetic containers had a structural problem in that outside air could not help but enter during use due to the structure in which the opening was opened and closed by a stopper, and furthermore, since the cosmetic composition was discharged by compression, there was a problem in that it was difficult to discharge the exact amount required each time.

Recently, in order to solve various problems such as these, a double-structured cosmetic container composed of a tubular inner container and a hard outer container, and equipped with an airless pump at the opening, has been developed.

Although the structure of a double-structured cosmetic container equipped with an airless pump has the advantage of being able to always dispense a fixed amount of cosmetic liquid while fundamentally preventing the introduction of contaminants from the outside, there was a problem of inconvenience in use because the cosmetic composition dispensed through the nozzle had to be applied to the skin by hand by the user.

In addition, if the cosmetic composition is applied to the skin without washing the hands thoroughly, it can cause bacterial infection of the skin, and in some cases, it can be difficult to evenly apply the cosmetic composition.

To solve this problem, a roller-type cosmetic container has been proposed in which a separate roller that rotates idly is installed in the opening of a tubular storage container so that a cosmetic composition discharged from the storage container is deposited on the surface of the roller and effectively applied to the user's skin.

However, these conventional roller-type cosmetic containers have their own advantages in that they apply the cosmetic composition discharged from the storage container to the surface of a rotating roller and then to the skin. Since the roller with the cosmetic composition applied is rubbed against the skin, not only can the cosmetic composition be applied in a cleaner state than when applied by hand, but also the cosmetic composition can be applied at a constant thickness.

However, since the discharged cosmetic composition clumps up and is discharged on the surface of the roller, there is a problem in that the cosmetic composition clumps up when the roller touches the user's skin to apply it, and there is the inconvenience of having to look at a separate mirror and use a roller-type cosmetic to apply it evenly and thinly on the skin.

In addition, cosmetic compositions applied to existing roller-type cosmetics have low skin adhesion and skin percutaneous absorption rate, and poor formulation stability, which limits the application of functional cosmetics such as sunscreens to roller-type cosmetics.

In addition, cosmetic containers such as compacts or eye shadows are generally produced with the upper and lower parts of the container body and one side of the lid hinged together, an inner plate and a powder case built into the container body, and a mirror attached to the inside of the lid.

Accordingly, the user can apply makeup while visually checking the area they wish to apply makeup to using a mirror attached to the inside of the cosmetic container, and such mirrors are essential to general cosmetic containers.

This type of mirror is made by repeatedly cutting a large mirror plate into a certain shape and then bonding and fixing it to the inside of a cosmetic container. However, in the process of attaching the mirror plate to the cosmetic container, the worker's fingerprints are left behind and dust or foreign substances easily stick to it. As a result, the worker has to be very careful, and if this is not confirmed during the inspection process, a claim may arise from the consumer, and there is a disadvantage in that the convenience of use for the consumer is reduced.

Republic of Korea Publication Patent No. 10-2022-0110955 (Published on 2022.08.09)

The present invention is characterized by developing a functional biomaterial effective for improving itchiness by strengthening the skin barrier by manufacturing a micelle to which functional peptides and ceramides having excellent moisturizing properties and tissue regeneration properties for the recovery of damaged skin barriers can be added at a high concentration based on a centrifugal microfluidic reactor, and applying the same to manufacture a multi-functional cosmetic composition having acquired itchiness improvement efficacy certification, skin-irritation certification, and triple functionality, and intends to apply it to a roller-type cosmetic container for convenient use. The roller-type cosmetic container has a mirror function, so that it is convenient to use and can provide various functional cosmetics in the form of sunscreen, BB cream, etc., and the pumped cosmetic liquid can be applied very gently to the skin and is a nanoemulsion cosmetic composition having improved skin adhesion, stability, and percutaneous absorption rate. The present invention also provides a method for manufacturing the nanoemulsion cosmetic composition and a cosmetic product including the roller-type container containing the cosmetic composition.

In the present invention, in order to overcome the limitations of liposome synthesis technology, research and development was conducted on a micelle synthesis method using a centrifugal microfluidic reactor to uniformly manufacture micelles of less than several hundred nm, and to develop a multi-functional cosmetic composition using micelles loaded with functional effective ingredients by utilizing the micelles.

A method for producing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor according to an embodiment of the present invention is a method for producing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor, characterized in that the centrifugal microfluidic reactor comprises: an injection unit having at least two microchannels through which a peptide solution and a ceramide solution are respectively injected; a junction unit having a microneedle that connects the microchannels of the injection unit into one; a crosslinking unit configured to surround and protect the junction unit and include a collection tube coupled to the injection unit; and a protective cap coupled to the injection unit and the crosslinking unit to cover and protect the injection unit and the crosslinking unit.

The above centrifugal microfluidic reactor is mounted on a centrifuge, and the rotational speed of the centrifuge is controlled in the range of 1,000 to 6,000 rpm.

Fluid A containing the peptide solution and fluid B containing the ceramide solution are injected into each of at least two injection portions having microchannels, and then Janus microparticles in which two or more different components are implemented as a single particle are synthesized by controlling the diameter of the microneedles at the junction and controlling the centrifugal force.

A nanoemulsion cosmetic composition according to an embodiment of the present invention is manufactured by a method for manufacturing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor.

The above nanoemulsion cosmetic composition comprises 0.8 to 20 wt% of the peptide-ceramide-containing nanoparticle micelle, 1.0 to 10.0 wt% of an ionic surfactant, 0.1 to 4.0 wt% of a fatty acid, 0.5 to 3.0 wt% of a stabilizer, 6 to 40 wt% of an oil component, 10 to 50 wt% of a skin physiologically active ingredient, and the remaining amount of a polyol.

The above peptide-ceramide containing nanoparticle micelle is a peptide-ceramide nanoparticle micelle containing 0.3 to 10.0 wt% of the peptide and 0.5 to 10.0 wt% of the ceramide.

A method for manufacturing a nanoemulsion cosmetic composition according to an embodiment of the present invention is characterized by including the following steps: 1) heating an aqueous component including peptide-ceramide-containing nanoparticle micelles, an ionic surfactant, a stabilizer, a fatty acid, and a polyol to 30 to 90°C; 2) adding and mixing an oil phase component to the heated aqueous component to form a dispersion; 3) homogenizing the dispersion using a high-pressure emulsifier to form a nanoemulsion; and 4) forming a cosmetic composition including the nanoemulsion.

In the above second step, the oil component includes an oil component and a skin physiologically active component.

The above nanoemulsion cosmetic composition is used in any one of sunscreen, lotion, essence and cushion cosmetics that have obtained triple functional cosmetics certification for whitening, wrinkle improvement and UV protection, certification for itchiness improvement by strengthening the skin barrier and certification for non-irritation of the skin.

A roller-type cosmetic container according to an embodiment of the present invention comprises: a container body containing a nanoemulsion cosmetic composition; a contents outlet coupled to the container body; a roller coupled to a roller coupling portion located above the contents outlet; and a mirror-shaped container lid covering the container body and having chrome deposited thereon; wherein the mirror-shaped container lid comprises a molded body composed of a biodegradable polymer mixed resin; an antibacterial coating layer formed over the entire inner surface of the molded body; and a chrome deposited layer formed over the entire outer surface of the molded body.

The above biodegradable polymer mixed resin is characterized by including: a mixed resin including an aliphatic polyester resin, carrageenan, and alginic acid; a thermoplastic elastomer; a cellulose fine powder having an average particle size of 1.0 to 10.0 ㎛; a filler having an average particle size of 0.1 to 50.0 ㎛; and an additive.

The aliphatic polyester resin comprises polylactic acid, the thermoplastic elastomer comprises at least one selected from styrene-(ethylene-propylene), styrene-(ethylene-butylene)-styrene, styrene-(ethylene-propylene)-styrene and styrene-(ethylene-butylene)-styrene-(ethylene-butylene), the filler comprises at least one selected from mica, talc and montmorillonite, and the additive comprises at least one selected from an internal lubricant, a plasticizer, a heat stabilizer, an antioxidant, a light stabilizer, a pigment and a dye.

The above biodegradable polymer mixed resin contains 20 to 40 parts by weight of a thermoplastic elastomer, 5 to 15 parts by weight of a cellulose fine powder, 1 to 5 parts by weight of a filler, and 2 to 10 parts by weight of an additive, based on 100 parts by weight of the mixed resin, and the mixed resin contains an aliphatic polyester resin, carrageenan, and alginic acid in a weight ratio of 1:0.2 to 0.4:0.05 to 0.15.

The above antibacterial coating layer is formed of an antibacterial coating agent including a polylactic acid resin, titanium dioxide, ginger extract, grapefruit seed extract, a silane coupling agent, a thickener, and an isopropyl alcohol aqueous solution.

The above antibacterial coating agent comprises 50 to 60 wt% of the polylactic acid resin, 1 to 3 wt% of titanium dioxide, 0.5 to 1.0 wt% of ginger extract, 0.5 to 1.5 wt% of grapefruit seed extract, 5 to 10 wt% of a silane coupling agent, 1 to 3 wt% of a thickener, and an isopropyl alcohol aqueous solution with the remaining balance among 100 wt%.

A roller-type cosmetic product according to an embodiment of the present invention is characterized by including a roller-type cosmetic container; and a cosmetic containing a nanoemulsion cosmetic composition accommodated in the roller-type cosmetic container.

A cosmetic containing the above nanoemulsion cosmetic composition is in a cream formulation, and the cosmetic containing the above nanoemulsion cosmetic composition has a viscosity of 5,000 to 60,000 cps at 25°C.

According to the present invention, a nanoemulsion cosmetic composition having triple functionality of anti-itching and anti-itching can be manufactured by efficiently using a nanoemulsion cosmetic composition having excellent skin adhesion, formulation stability, and percutaneous absorption rate.

In addition, according to the present invention, a roller-type cosmetic product can be provided by applying a nanoemulsion cosmetic composition to a roller-type cosmetic container comprising a biodegradable mirror-shaped container lid.

In addition, according to the present invention, in developing a nanoemulsion cosmetic composition having triple functionality of skin barrier protection, moisturizing effect, and anti-itching effect by containing nanoliposomes stabilized by containing peptides and ceramides as effective ingredients, a new cosmetic formulation technology can be developed using a centrifugal microfluidic reactor to disperse and stabilize various functional biomaterials that must exist in high concentrations under low viscosity liquid conditions.

To this end, the centrifugal microfluidic reactor of the present invention not only has the characteristic of being able to particleize micelles, Janus, and core-shells of various sizes using centrifugal force, but also enables rapid formulation with excellent dispersion stability under centrifugal force conditions.

In addition, the centrifugal microfluidic reactor of the present invention can control the desired ratio and amount of functional components to be included inside the core of micelles, and can maximize the functional effect by dividing them into particles with various structures. Specifically, by varying the combination of solutions put into the injection portion, various types of micelles containing functional components can be manufactured.

As a result, according to the present invention, a functional biomaterial formulation technology using a centrifugal microfluidic reactor was developed, and a multi-functional cosmetic composition with an itching improvement effect was developed by strengthening the skin barrier and including triple functional effective ingredients of whitening, wrinkle improvement, and UV protection.

In addition, according to the present invention, a functional peptide and ceramide having excellent moisturizing and tissue regeneration abilities for recovering damaged skin barriers were manufactured as nanoparticle micelles using a centrifugal microfluidic reactor to formulate a functional nano biomaterial effective in improving itchiness by strengthening the skin barrier.

In addition, according to the present invention, by applying nanoparticle micelles containing peptide-ceramide that alleviate atopic symptoms and itchy skin symptoms, in addition to the triple functionality of UV protection, whitening, and wrinkle improvement, it is attractive for use by customers complaining of atopic symptoms and customers who value cosmetic ingredients.

In addition, according to the present invention, peptides and ceramides are stabilized as micelles of nanoparticles, and applied to cosmetics to improve formulation stability and percutaneous absorption rate for active ingredients, and can be usefully used as a nanoemulsion cosmetic composition having triple functionality of itching improvement, whitening, wrinkle improvement, and UV protection.

In addition, according to the present invention, it can be used safely without exhibiting cytotoxicity, can be used safely even for long-term use, and can be effectively used for preventing dermatitis and improving symptoms.

In addition, the roller-type cosmetic container of the present invention has a chrome deposition layer formed on the entire exterior of the mirror-shaped container lid, so that the container lid itself has a mirror function, making it convenient for the user to use.

In addition, the roller-type cosmetic product of the present invention has a smaller particle size than existing micro-emulsion cosmetic compositions, so it has excellent skin adhesion and percutaneous absorption rate, and includes a cosmetic manufactured with a cosmetic composition having excellent formulation stability, so that it can provide cosmetics in various formulations such as sunscreen and BB cream.

Figure 1 is a process flow diagram showing a method for manufacturing a nanoemulsion cosmetic composition according to an embodiment of the present invention.
Figure 2 is a perspective view showing a centrifugal microfluidic reactor according to an embodiment of the present invention.
Figure 3 is a drawing showing microparticles and microfibers synthesized when the length (L) to the upper surface of the aqueous solution accommodated in the cross-linking section of the centrifugal microfluidic reactor of Figure 2 is 2 cm, 1 cm, and -1 cm.

Hereinafter, preferred embodiments of the present invention and the properties of each component will be described in detail. However, this is intended to be a detailed description to enable a person having ordinary skill in the art to which the present invention pertains to easily carry out the invention, and does not mean that the technical idea and scope of the present invention are limited thereby.

First, the nanoemulsion cosmetic composition, which is the content of a cosmetic product using the roller-shaped container of the present invention, will be described.

An emulsion is a liquid that does not dissolve in each other, such as water and oil, dispersed as fine particles in another liquid. In general, emulsions are divided into microemulsions (10 to 100 nm), nanoemulsions or miniemulsions (20 to 500 nm), and macroemulsions (1 to 100 μm) depending on particle size.

The cosmetic composition of the present invention is a nanoemulsion type cosmetic, and the particle size of the nanoemulsion constituting the cosmetic composition is 20 to 50 nm, preferably 100 to 20 nm.

A nanoemulsion cosmetic composition having such a particle size has a uniform particle size distribution of the nanoemulsion, is a milky white, translucent formulation, has no discoloration or odor, has high-temperature long-term stability, and has excellent water dispersibility, so that it can be applied to various types of cosmetics, such as skin, lotion, cream, and essence formulations, and thus its utility can be maximized.

A nanoemulsion cosmetic composition according to an embodiment of the present invention is manufactured by a method for manufacturing peptide-ceramide-containing nanoparticle micelles using a centrifugal microfluidic reactor.

To this end, the nanoemulsion cosmetic composition according to an embodiment of the present invention comprises 0.8 to 20 wt% of peptide-ceramide-containing nanoparticle micelles, 1.0 to 10.0 wt% of ionic surfactant, 0.1 to 4.0 wt% of fatty acid, 0.5 to 3.0 wt% of stabilizer, 6 to 40 wt% of oil component, 10 to 50 wt% of skin physiologically active component, and the remaining balance of polyol.

Peptide-ceramide containing nanoparticle micelles contain peptide: 0.3 to 10.0 wt% and ceramide: 0.5 to 10.0 wt%.

Peptides have excellent skin regeneration ability, promote wound healing, enhance skin elasticity, and increase the subcutaneous fat layer to prevent skin aging, thereby preventing and improving atopic skin disease. It is preferable to use a functional biomaterial containing at least one selected from Copper Tripeptide-1, Palmitoyl Tripeptide-1, and Carnosine as the peptide. It is more preferable that Copper Tripeptide-1, Palmitoyl Tripeptide-1, and Carnosine are all added to these peptides.

It is preferable that the content of the peptide in the nanoemulsion cosmetic composition be 0.3 to 10.0 wt%, more preferably 0.6 to 8.0 wt%, and even more preferably 3.0 to 7.0 wt%. At this time, if the content of the peptide in the nanoemulsion cosmetic composition is less than 0.3 wt%, there may be a problem in that it is difficult to exhibit efficacy, and if it is used in excess of 10.0 wt%, the formulation stability of the nanoemulsion is very unstable, and a precipitation phenomenon may occur, so it is recommended to use it within the above range.

It is preferable to use natural ceramide for ceramide. These ceramides form a skin protective layer to block external harmful substances, prevent moisture from escaping from the skin, strengthen the skin barrier, and promote anti-aging of the skin. In this way, ceramide is a human-identical ceramide obtained through yeast fermentation, and is structurally different from similar ceramides made by synthesizing fatty acids and cholesterol, and has different physical properties such as melting point and hydrophobicity, making it more difficult to stabilize natural ceramides into nanoemulsions.

It is preferable that the content of ceramide in the nanoemulsion cosmetic composition be 0.5 to 10.0 wt%, more preferably 0.5 to 9.0 wt%, and even more preferably 2.0 to 8.0 wt%. At this time, if the content of ceramide in the nanoemulsion cosmetic composition is less than 0.5 wt%, there may be a problem in that it is difficult to exhibit efficacy, and if it is used in excess of 10.0 wt%, the formulation stability of the nanoemulsion may be very unstable, causing a precipitation phenomenon, so it is recommended to use it within the above range.

Nanoemulsion production can be largely divided into a mechanical method (High-energy Emulsification) using a microfluidizer and a surfactant method (Low Energy Emulsification) using D-phase emulsification, liquid crystal emulsification, PIT emulsification, and PIC emulsification. However, since the surfactant method must pass through a region where an infinite association of surfactants (liquid crystal phase, D phase) is formed, which is a single-phase region where the surface tension is theoretically 0 or close to 0 during the emulsification process, there are many restrictions on containing various useful components due to the types and compositions of surfactants, oil phases, and water phases, emulsification temperature, and cooling conditions.

Therefore, high-pressure emulsification is generally used to stably produce nanoemulsions containing various useful ingredients using a minimum of surfactants. The process conditions and selection of emulsifiers are important in producing such nanoemulsions. Nanoemulsions can be produced by combining various emulsifiers, and when using a high-pressure microfluidizer, it is preferable to use phospholipids or lecithin as the main surfactant.

Since these ceramides have different physical properties such as melting point and hydrophobicity, it is more difficult to stabilize them as nanoemulsions, so a method for easily manufacturing peptide-ceramide microparticle micelles in a Janus shape using a centrifugal microfluidic reactor was applied.

As a stabilizer, sodium surfactin, sodium dextran sulfate or a mixture thereof is included in an amount of 0.5 to 3.0 wt%, more preferably 1.0 to 2.0 wt%, based on the total weight of the nanoemulsion.

The ionic surfactant may be selected from the group consisting of Sodium Stearoyl Glutamate, Sodium Lauryl Glutamate, Potassium Cetyl Phosphate, Glyceryl Stearate Citrate, and Distearyl Dimonium Chloride, and used alone or in combination of two or more thereof, more preferably Sodium Stearoyl Glutamate.

At this time, it is appropriate to add the ionic surfactant at 1 to 10 wt%, more preferably 2 to 6 wt%, based on the total weight of the nanoemulsion cosmetic composition. If the ionic surfactant is included at less than 1.0 wt%, the stabilizing effect is insignificant when applied as a cosmetic formulation, and particle formation may not occur. If it exceeds 10.0 wt%, skin irritation may occur, so it is appropriate to adjust it within the above range.

In addition, polyol is used to increase transparency by adjusting the refractive index of the formulation and to improve the stability of the formulation by lowering the freezing point, and can be used alone or in combination of one or more selected from the group consisting of glycerin, 1,3-butylene glycol, propylene glycol, pentylene glycol, propanediol, methyl propanediol, and dipropylene glycol.

The purpose of the present invention is to provide a nanoemulsion cosmetic composition containing a high content of multi-functional ingredients, having a uniform particle size distribution, and excellent stability and water dispersibility.

In addition, the nanoemulsion cosmetic composition contains at least one useful ingredient selected from the group consisting of silicone oil, ester oil, hydrocarbon oil, vegetable oil, vegetable butter, wax, sunscreen, and useful skin physiologically active ingredient in an amount of 10 to 60 wt%, preferably 20 to 50 wt%, based on the total weight of the nanoemulsion.

The useful skin physiologically active ingredient is at least one selected from the group consisting of ubiquinone, astaxanthin, salicylic acid, azelaic acid, menthol, sphingosine, ursolic acid, and tetraacetylphytosphingosine, and is contained in an amount of 0.001 to 30 wt% based on the total weight of the nanoemulsion cosmetic composition. The nanoemulsion cosmetic composition is characterized by having a low viscosity liquid to paste property and being easily dispersed in an aqueous phase.

Figure 1 is a process flow diagram showing a method for manufacturing a nanoemulsion cosmetic composition according to an embodiment of the present invention.

As shown in Fig. 1, a method for manufacturing a nanoemulsion cosmetic composition according to an embodiment of the present invention includes a step of heating an aqueous component (S1), a step of introducing and mixing an oily component (S2), and a high-pressure emulsification step (S3).

In the water component heating step (S1), the first step is to heat the water component including peptide-ceramide-containing nanoparticle micelles, ionic surfactants, stabilizers, fatty acids, and polyols to 30 to 90°C.

In this water component heating step (S1), the peptide-ceramide containing nanoparticle micelles are centrifuged in a microfluidic reactor.

In the oil phase component addition and mixing step (S2), the second step of forming a dispersion is performed by adding and mixing the oil phase component into the heated water phase component.

In the high-pressure emulsification step (S3), the dispersion is homogenized using a high-pressure emulsifier to form a nanoemulsion, which is the third step.

In the oil component input and mixing step (S2), the oil component includes an oil component and a skin physiologically active ingredient.

Nanoemulsion cosmetic compositions are used in any one of sunscreens, lotions, essences, and cushions that have acquired triple-functional cosmetics certification for whitening, wrinkle improvement, and UV protection, certification for itchiness improvement by strengthening the skin barrier, and certification for non-irritation of the skin.

Meanwhile, a method for manufacturing a nanoparticle micelle according to an embodiment of the present invention is a method for manufacturing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor, wherein the centrifugal microfluidic reactor comprises: an injection unit having at least two microchannels through which a peptide solution and a ceramide solution are respectively injected; a junction unit having a microneedle that connects the microchannels of the injection unit into one; a crosslinking unit configured to surround and protect the junction unit and include a collection tube coupled to the injection unit; and a protective cap coupled to the injection unit and the crosslinking unit to cover and protect the injection unit and the crosslinking unit.

The centrifugal microfluidic reactor is mounted on a centrifuge, and the rotational speed of the centrifuge is controlled in the range of 1,000 to 6,000 rpm.

Here, fluid A containing a peptide solution and fluid B containing a ceramide solution are injected into each of an injection unit having at least two microchannels, and then Janus microparticles in which two or more different components are implemented as a single particle are synthesized by controlling the diameter of the microneedles at the junction and controlling the centrifugal force.

FIG. 2 is a perspective view showing a centrifugal microfluidic reactor according to an embodiment of the present invention, and FIG. 3 is a drawing showing microparticles and microfibers synthesized when the length (L) to the upper surface of the aqueous solution accommodated in the cross-linking portion of the centrifugal microfluidic reactor of FIG. 2 is 2 cm, 1 cm, and -1 cm.

As shown in FIGS. 2 and 3, a centrifugal microfluidic reactor according to an embodiment of the present invention comprises: an injection unit (10) having at least two microchannels, through which a peptide solution and a ceramide solution are respectively injected; a junction unit (20) having a microneedle that connects the microchannels of the injection unit (10) into one; a bridge unit (30) configured as a collection tube coupled to the injection unit (20) and protecting the junction unit (20); and a protective cap (31) coupled to the injection unit (20) and the bridge unit (30) to cover and protect the injection unit (20) and the bridge unit (30).

A centrifugal microfluidic reactor having this configuration can synthesize various microparticles in the form of micelles through synthesis and structural control, and a representative form is obtained as a Janus-shaped microparticle micelle by synthesizing through two injection ports (10).

In addition, if several microneedles are gathered together to form a system, it is possible to form microparticles with three, four, or more substances. In addition, if a system with a structure in which microneedles are placed within microneedles is used, particles in which one substance is wrapped around another substance can be created, and particles of various compositions and shapes can be formed depending on the configuration of the system (number of microneedles, method of integration, etc.). In addition, the size and shape of the particles (sphere, long tube, fiber) can be controlled by controlling the diameter of the microneedle and the distance between the needle tip and the aqueous solution (2 cm, 1 cm, -1 cm).

The nanoemulsion cosmetic composition according to an embodiment of the present invention is used in a basic cosmetic composition selected from skin, lotion, cream, essence, etc., a color cosmetic selected from foundation, and a functional cosmetic such as a UV blocker.

A cosmetic containing such a nanoemulsion cosmetic composition comprises sodium stearoyl glutamate as a surfactant, sodium surfactin as a stabilizer, and at least one oil-soluble UV blocking agent selected from the group consisting of butyl methoxy dibenzoylmethane, bis-ethylhexyloxy phenol methoxyphenyl triazine, and diethylaminohydroxybenzoyl hexyl benzoate, and is a cosmetic having a nanoemulsion formulation.

The nanoparticle micelle according to the present invention, which is prepared by using sodium stearoyl glutamate as a surfactant and sodium surfactin, sodium dextran sulfate or a mixture thereof as a stabilizer, and a nanoemulsion cosmetic composition containing the same, contains a high content of a useful ingredient, has a particle size of 100 to 200 nm and a uniform particle size distribution, and has no discoloration in appearance, no odor, and no layer separation, and dramatically improves long-term stability at high temperatures. In addition, it has excellent water dispersibility and can be applied to all formulations, so that the usability of the nanoemulsion can be maximized.

A roller-type cosmetic container according to an embodiment of the present invention comprises: a container body containing a nanoemulsion cosmetic composition; a contents outlet coupled to the container body; a roller coupled to a roller coupling portion located above the contents outlet; and a mirror-shaped container lid covering the container body and having chrome deposited thereon; wherein the mirror-shaped container lid comprises a molded body composed of a biodegradable polymer mixed resin; an antibacterial coating layer formed on the entire inner surface of the molded body; and a chrome deposited layer formed on the entire outer surface of the molded body.

The assembly method and usage conditions of the roller-type cosmetic container of the present invention are described in detail as follows.

First, in order to assemble a roller-type cosmetic container, a contents outlet is formed on the top of the container body containing the contents, and the contents outlet is connected. After that, a roller provided in the roller connection part is connected to the contents outlet, and then the container lid is connected to complete the assembly.

To use the roller-type cosmetic container assembled in the above manner, press and hold the contents discharge button protruding from the contents discharge port on the top of the container body, and the contents contained in the container body will pass through the discharge pipe of the contents discharge port and be applied to the roller. The contents will be applied to the skin and evenly spread by rubbing the roller, allowing convenient use.

You can use it while looking at the mirror with chrome deposition on the container lid while checking that it is applied evenly through the roller.

For this purpose, cosmetic containers with mirror-shaped container lids are used.

The mirror-shaped container lid of these cosmetic containers comprises a molded body composed of a biodegradable polymer blend resin; an antibacterial coating layer formed over the entire inner surface of the molded body; and a chrome deposition layer formed over the entire outer surface of the molded body.

The molded body refers to a molded body made by molding a biodegradable polymer mixed resin into a lid shape, and is environmentally friendly as it contains a biodegradable polymer as its main component.

The biodegradable polymer blend resin includes a blend resin comprising an aliphatic polyester resin, carrageenan and alginic acid; a thermoplastic elastomer; a cellulose micropowder; a filler; and an additive.

Among the biodegradable polymer blend resin components, the aliphatic polyester resin may include polylactic acid, and the blend resin may include the aliphatic polyester resin, carrageenan, and alginic acid in a weight ratio of 1:0.2 to 0.4:0.05 to 0.15, preferably 1:0.25 to 0.34:0.05 to 0.10.

And, among the biodegradable polymer mixed resin components, the thermoplastic elastomer is intended to supplement the mechanical properties such as rigidity and impact resistance that cannot be satisfied when manufacturing a molded article with only the biodegradable resin, and to increase heat resistance and moldability, and may include at least one selected from styrene-(ethylene-propylene), styrene-(ethylene-butylene)-styrene, styrene-(ethylene-propylene)-styrene, and styrene-(ethylene-butylene)-styrene-(ethylene-butylene), and preferably may include at least one selected from styrene-(ethylene-propylene), styrene-(ethylene-butylene)-styrene, and styrene-(ethylene-propylene)-styrene.

And, the amount of thermoplastic elastomer to be used may be 20 to 40 parts by weight, more preferably 22 to 35 parts by weight, and even more preferably 24 to 32 parts by weight, per 100 parts by weight of the mixed resin. At this time, if the amount of thermoplastic elastomer used is less than 20 parts by weight, the mechanical properties of the container lid may be insufficient and there may be problems with poor moldability, and if it exceeds 40 parts by weight, there may be problems with significantly reduced biodegradability of the container lid, so it is recommended to use it within the above range.

And, among the biodegradable polymer mixed resin components, the cellulose fine powder is a biodegradable material that improves the mechanical properties of the molded body, which is the container lid, and is manufactured by crushing and drying paper dust generated during the manufacture of paper products such as pulp and cardboard, and can be used having an average particle size of 1.0 to 10.0 ㎛. And, the amount of the cellulose fine powder to be used is 5 to 15 parts by weight, more preferably 7 to 13 parts by weight, and even more preferably 8.0 to 12.5 parts by weight, per 100 parts by weight of the mixed resin, which is advantageous in terms of improving the moldability and proper properties of the biodegradable polymer mixed resin.

And, among the biodegradable polymer mixed resin components, the filler plays a role of improving the wear resistance of the molded body, which is the container lid, and may include at least one selected from mica, talc, and montmorillonite. And, it is appropriate to use the filler having an average particle size of 0.1 to 50.0 ㎛, more preferably an average particle size of 2.0 to 30.0 ㎛. And, the amount of the filler to be used is appropriate to use 1 to 5 parts by weight, more preferably 1.5 to 4.5 parts by weight, and even more preferably 2.0 to 4.0 parts by weight, with respect to 100 parts by weight of the mixed resin.

And, among the biodegradable polymer mixed resin components, the additive may include at least one selected from an internal activator, a plasticizer, a heat stabilizer, an antioxidant, a light stabilizer, a pigment, and a dye. And, the amount of the additive to be used is preferably 10 parts by weight or less, more preferably 2 to 10 parts by weight, and even more preferably 5.0 to 10.0 parts by weight, based on 100 parts by weight of the mixed resin, in terms of biodegradability, mechanical properties, etc. of the container lid.

The biodegradable polymer blend resin manufactured with the above components and component ratio preferably has a melt flow index of 0.2 to 1.0 g/10 min, more preferably 0.4 to 0.9 g/10 min, as measured at 230° C. and a load of 2.16 kg according to ASTM-D1238. If the melt flow index of the biodegradable polymer blend resin is less than 0.2 g/10 min, the thickness formability is poor, and if it exceeds 1.0 g/10 min, the flowability is too high, which may cause problems such as deterioration of mechanical properties and poor formability.

Additionally, the entire inside of the mirror-shaped container lid is coated with an antibacterial coating agent to form an antibacterial coating layer.

The antibacterial coating layer, like the container body itself, is made of an environmentally friendly material as it is biodegradable, and the antibacterial coating agent includes a polylactic acid resin, titanium dioxide, ginger extract, grapefruit seed extract, a silane coupling agent, a thickener, and an isopropyl alcohol aqueous solution, preferably 50 to 60 wt% of the polylactic acid resin, 1 to 3 wt% of the titanium dioxide, 0.5 to 1.0 wt% of the ginger extract, 0.5 to 1.5 wt% of the grapefruit seed extract, 5 to 10 wt% of the silane coupling agent, 1 to 3 wt% of the thickener, and the remainder of the isopropyl alcohol aqueous solution, and more preferably 52 to 58 wt% of the polylactic acid resin, 1.5 to 2.5 wt% of the titanium dioxide, 0.5 to 1.0 wt% of the ginger extract, and 1.0 to 1.5 wt% of the grapefruit seed extract. It contains 7.0 to 9.5 wt% of a silane coupling agent, 1.0 to 2.0 wt% of a thickener, and the remainder is an isopropyl alcohol aqueous solution.

Among the antibacterial coating ingredients, the silane coupling agent improves the reactivity of the antibacterial coating layer and the adhesion and bonding strength with the molded body, and may include at least one selected from amino silane, epoxy silane, and tetraethoxy silane. In addition, if the silane coupling agent is less than 5 wt%, the surface hardness is weak, and if it exceeds 10 wt%, problems such as reduced coating properties and reduced bonding strength may occur, such as bubbles or peeling.

Among the antibacterial coating ingredients, the thickener promotes coagulation of the composition in the antibacterial coating agent and plays a role in controlling viscosity, and methyl cellulose or hydroxyethyl cellulose can be used. If the thickener is less than 1 wt%, it is difficult to expect a viscosity-increasing effect due to the use of the thickener, and if it is used in excess of 3 wt%, the problem of significantly reduced fluidity may occur due to the high viscosity.

Additionally, the antimicrobial coating agent may further include one or more selected from among a fouling improvement agent, a dispersant, and a leveling agent.

And, a preferred example of a method for manufacturing a roller-type cosmetic container and a method for forming a chrome deposition layer on a container lid is as follows.

A cosmetic container can be manufactured by performing a process including: 1st step of injection molding and washing and drying; 2nd step of removing static electricity and foreign substances by performing static and dust removal; 3rd step of preheating the container; 4th step of forming a character printing layer through silk printing on the surface of the container; 5th step of forming a UV coating layer on top of the character printing layer; 6th step of forming a metal deposition layer on top of the UV coating layer; 7th step of forming a UV coating or urethane coating layer; and 8th step of performing UV drying or heat drying.

Here, the first step is a process of injection molding a container and washing and drying it. PC, ABS, or acrylic are used as materials, and the injection molded container can be washed using a detergent such as normal hexane, or washed using ultrasonic waves or distilled water, and then dried with heat.

Additionally, step 1 can also perform laser marking work to express various patterns or phrases on the surface of the washed and dried container.

Next, the second stage is a process for static and dust removal, in which a brush is rotated in contact with the container to remove static electricity and foreign substances, and compressed air is sprayed into the container to remove foreign substances such as dust.

Next, the third step is a process of pre-treating the container before forming a character printing layer on the container. The container is transported at approximately 30 m/min and preheated to 40 to 60°C so that the character printing layer can be smoothly silk-printed.

Next, the fourth step is a process of forming a character printing layer representing a brand or logo by silk printing or pad printing ink on a portion of the upper surface of the container. At this time, the ink may include a polyurethane resin, isoamyl alcohol, a glycol derivative organic solvent, methyl isobutyl ketone, and a pigment, and preferably, with respect to 100 parts by weight of the polyurethane resin, it may include 50 to 70 parts by weight of isoamyl alcohol, 20 to 40 parts by weight of the glycol derivative organic solvent, 20 to 40 parts by weight of methyl isobutyl ketone, and 5 to 25 parts by weight of the pigment.

Methyl isobutyl ketone activates the polyurethane resin to enhance the three-dimensional effect by bringing out the relief pattern of the character printing layer, and methyl salicylate may be added, and the weight ratio of methyl isobutyl ketone to methyl salicylate is preferably 1:0.5 to 1.

And, after silk printing, drying is performed at 60 to 80℃.

Next, step 5 is the process of forming a UV coating layer. UV coating is performed on a container on which a character printing layer has been formed, and then UV drying is performed.

Next, step 6 is a process for forming a metal deposition layer on the upper surface of the UV coating layer. This process is to place a container and metal particles to be attached to the surface in a high vacuum furnace, then apply current to a heater to heat the container, thereby evaporating the metal particles, and attaching the metal skin by condensing them on the surface of a cold container. At this time, the metal particles may include chromium, aluminum, or tin.

And, the container lid can be processed by performing steps 1 to 5, similarly to other cosmetic containers, and in step 6, the entire upper surface of the container lid can be deposited with chrome to create a mirror effect.

Next, in step 7, in order to prevent damage to the metal deposition layer and ensure reliability, a UV coating solution or urethane coating solution mixed with phytoncide essential oil is applied to the upper surface of the metal deposition layer, and UV drying or heat drying at 60 to 85°C is performed to manufacture a cosmetic container and a container lid.

The roller-type cosmetic product according to the embodiment of the present invention described above is a cosmetic contained in a roller-type cosmetic container, and can provide cosmetics such as sunscreen and BB cream that are easy to apply and convenient to use.

As described above, according to the present invention, a nanoemulsion cosmetic composition having triple functionality of anti-itching effect can be manufactured by efficiently using a nanoemulsion cosmetic composition having excellent skin adhesion, formulation stability, and percutaneous absorption rate.

In addition, according to the present invention, a roller-type cosmetic product can be provided by applying a nanoemulsion cosmetic composition to a roller-type cosmetic container comprising a biodegradable mirror-shaped container lid.

In addition, according to the present invention, in developing a nanoemulsion cosmetic composition having triple functionality of skin barrier protection, moisturizing effect, and anti-itching effect by containing nanoliposomes stabilized by containing peptides and ceramides as effective ingredients, a new cosmetic formulation technology can be developed using a centrifugal microfluidic reactor to disperse and stabilize various functional biomaterials that must exist in high concentrations under low viscosity liquid conditions.

To this end, the centrifugal microfluidic reactor of the present invention not only has the characteristic of being able to particleize micelles, Janus, and core-shells of various sizes using centrifugal force, but also enables rapid formulation with excellent dispersion stability under centrifugal force conditions.

In addition, the centrifugal microfluidic reactor of the present invention can control the desired ratio and amount of functional components to be included inside the core of micelles, and can maximize the functional effect by dividing them into particles with various structures. Specifically, by varying the combination of solutions put into the injection portion, various types of micelles containing functional components can be manufactured.

As a result, according to the present invention, a functional biomaterial formulation technology using a centrifugal microfluidic reactor was developed, and a multi-functional cosmetic composition with an itching improvement effect was developed by strengthening the skin barrier and including triple functional effective ingredients of whitening, wrinkle improvement, and UV protection.

In addition, according to the present invention, a functional peptide and ceramide having excellent moisturizing and tissue regeneration abilities for recovering damaged skin barriers were manufactured as nanoparticle micelles using a centrifugal microfluidic reactor to formulate a functional nano biomaterial effective in improving itchiness by strengthening the skin barrier.

In addition, according to the present invention, by applying nanoparticle micelles containing peptide-ceramide that alleviate atopic symptoms and itchy skin symptoms, in addition to the triple functionality of UV protection, whitening, and wrinkle improvement, it is attractive for use by customers complaining of atopic symptoms and customers who value cosmetic ingredients.

In addition, according to the present invention, peptides and ceramides are stabilized as micelles of nanoparticles, and applied to cosmetics to improve formulation stability and percutaneous absorption rate for active ingredients, and can be usefully used as a nanoemulsion cosmetic composition having triple functionality of itching improvement, whitening, wrinkle improvement, and UV protection.

In addition, according to the present invention, it can be used safely without exhibiting cytotoxicity, can be used safely even for long-term use, and can be effectively used for preventing dermatitis and improving symptoms.

In addition, the roller-type cosmetic container of the present invention has a chrome deposition layer formed on the entire exterior of the mirror-shaped container lid, so that the container lid itself has a mirror function, making it convenient for the user to use.

In addition, the roller-type cosmetic product of the present invention has a smaller particle size than existing micro-emulsion cosmetic compositions, so it has excellent skin adhesion and percutaneous absorption rate, and includes a cosmetic manufactured with a cosmetic composition having excellent formulation stability, so that it can provide cosmetics in various formulations such as sunscreen and BB cream.

Example

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, these are presented as preferred examples of the present invention and cannot be interpreted as limiting the present invention in any way.

Anything not described here is technically inferable enough to be understood by those skilled in the art, so its description will be omitted.

1. Preparation of nanoemulsion cosmetic composition

Example 1

A peptide solution containing copper tripeptide-1, palmitoyl tripeptide-1, and carnosine and a natural ceramide solution were injected into an injection unit having two microchannels, and then the microchannels of the injection unit were connected into one through a junction unit having a microneedle to synthesize the solution within a cross-linking unit, thereby producing a peptide-ceramide-containing nanoparticle micelle.

At this time, during synthesis, the centrifugal microfluidic reactor was mounted on a centrifuge, and the rotational speed of the centrifuge was adjusted to 4,000 rpm.

Next, the peptide-ceramide-containing nanoparticle micelle, sodium stearoyl glutamate as an ionic surfactant, sodium surfactin as a stabilizer, stearic acid as a fatty acid, and 1,3-butylene glycol as a polyol were mixed, and the mixture was heated to 75°C. Then, the oily components, ester oil, hydrocarbon oil, and skin physiologically active ingredients, ubiquinone and astaxanthin, were added to the heated aqueous component mixture, and then slowly stirred to prepare a mixed solution.

Next, the mixture was stirred at 3,000 rpm for 5 minutes using a homomixer, and then cooled to 40°C while stirring at 3,000 rpm to prepare a dispersion. Next, the dispersion was obtained by using a high-pressure microfluidizer to obtain a dispersion in which nanoemulsions having a particle size of 100 nm or less were homogeneously dispersed.

Example 2

A nanoemulsion cosmetic composition was prepared in the same manner as in Example 1, except that each component was added in the composition and composition ratio described in Table 1.

(Unit: Weight%) division Example 1 Example 2 Nanoparticles
Mycell Natural Ceramide 10.0 7.7 Functional Peptides Copper Tripeptide-1 2.0 1.5 Palmitoyl Tripeptide-1 2.0 1.5 Carnosine 2.0 1.0 Ionic surfactant 4.0 1.5 Stabilizer 2.0 0.8 fatty acid 3.0 3.5 Ester oil 15.0 15.0 Hydrocarbon oil 15.0 15.0 Skin physiological active ingredients 10.0 10.0 Polyol 100% by weight
Remaining balance 100% by weight
Remaining balance

2. Cosmetics manufacturing

The nanoemulsion cosmetic compositions manufactured in Examples 1 and 2 were mixed in the compositions and composition ratios of Table 2 to manufacture cosmetics according to Manufacturing Examples 1 and 2. In addition, a cosmetic according to Comparative Example 1 was manufactured by mixing in the compositions and composition ratios of Table 2 without adding the nanoemulsion cosmetic composition.

(Unit: Weight%) division Raw material name Manufacturing example 1 Manufacturing example 2 Comparative Example 1 Nanoemulsion Nanoemulsion cosmetic composition Example 1
Use15.00 Example 2
Use10.00 - Paid ingredients Cyclopentasiloxane 10.0 10.0 10.0 Phenyl trimethicone 3.0 3.0 3.0 Ethylhexyl palmitate 5.0 5.0 5.0 Ethylhexyl methoxycinnamate 7.5 7.5 7.5 Bisethylhexyloxyphenol methoxyphenyl triazine 3.0 3.0 3.0 Isoamyl paramethoxycinnamate 3.0 3.0 3.0 lecithin 0.1 0.1 0.1 Lauryl PEG-10 Tris(trimethylsiloxy)silylethyldimethicone 4.0 4.0 4.0 Thickener Disteardimonium hectorite 0.3 0.3 0.3 Powder
ingredient Titanium Dioxide*Aluminum Hydroxide
*Stearic acid 10.57 10.57 10.57 Zinc oxide*triethoxycaprylylsilane 9.7 9.7 9.7 iron oxide 1.2 1.2 1.2 Award winning ingredient 1,3-Butylene Glycol 6.0 6.0 6.0 Sodium chloride 0.5 0.5 0.5 glycerin 2.0 2.0 2.0 Phenoxyethanol 0.3 0.3 0.3 1,2-Hexanediol 2.0 2.0 2.0 Panthenol 0.01 0.01 0.01 Niacinamide 2.0 2.0 2.0 Adenosine 0.04 0.04 0.04 Tocopheryl acetate 0.01 0.01 0.01 Disodium EDTA 0.01 0.01 0.01 Purified water To 100 To 100 To 100

3. Stability Test

The harsh stability test of the formulation of cosmetics manufactured according to Manufacturing Examples 1 to 2 and Comparative Example 1 was conducted and is shown in Table 3. The cycle of the formulation stability test was measured after three tests of 8 hours each under the conditions of -5℃, 25℃, and 45℃.

division Manufacturing example 1 Manufacturing example 2 Comparative Example 1 Nanoemulsion cosmetics Example 1 Example 2 - Harsh
Stability
test
result Room temperature (25℃), 3 months Stability Stability Layer separation Refrigerated (5℃), 3 months Stability Stability Stability High temperature (40℃), 3 months Stability Stability Layer separation Cycle, 1 month Stability Stability Layer separation

As shown in Table 3, when examining the harsh stability test evaluation of cosmetics, both Manufacturing Example 1 using the nanoemulsion cosmetic composition of Example 1 and Manufacturing Example 2 using the nanoemulsion cosmetic composition of Example 2 using a small amount of ionic surfactant and stabilizer showed excellent results in formulation stability.

In contrast , in the case of the cosmetic of Comparative Example 1 manufactured without adding the nanoemulsion cosmetic composition, there was a problem of poor formulation stability in stability tests at room temperature (25°C), high temperature (40°C), and cycle (-5°C, 25°C, 45°C, 3 times for 8 h each).

4. Improvement of skin texture and measurement of skin keratin

Table 4 shows the results of improving skin texture and measuring skin keratin after applying cosmetics manufactured according to Manufacturing Examples 1 to 2 and Comparative Example 1 to the skin of test subjects. At this time, cosmetics manufactured according to Manufacturing Examples 1 to 2 and Comparative Example 1 were applied to the skin of test subjects, and the skin texture (roughness) of the test subjects was measured using an Antera device. The analysis area was designated in the measured photos, and the average roughness (Ra value) representing the skin roughness for each area was measured as the measurement value. A decrease in the result value means an improvement in skin texture. The average roughness was confirmed by comparing the measurement values before and after using sunscreen for 3 weeks, and expressing it as a change rate (%).

Change rate (%) = [(measurement value after use - measurement value before use) / (measurement value before use)] × 100

Probability of significance (p -value) *: Repeated Measures ANOVA (***: P <0.001)

To measure skin keratin, D-sqauam (keratin tape) was used to collect keratin from the skin of the test subject, and then photographs were taken using Visioscan VC98 (Courage-Khazaka Eletronic GmbH, Germany). The average keratin index (DI: Desquamation Index) of the photographs was measured. A decrease in the measured value indicates improvement. After using the test product sunscreen for 3 weeks, keratin was measured, and the DI (Desquamation Index) value was used as skin keratin evaluation data.

division Roughness change rate (%) Desquamation Index (DI) Manufacturing example 1 -13.41 7.32 Manufacturing example 2 -11.11 8.15 Comparative Example 1 -1.26 15.78

As shown in Table 4, the results of skin texture improvement and skin keratin measurement showed that when cosmetics manufactured according to Manufacturing Examples 1 and 2 were used, the skin condition was significantly improved compared to when cosmetics manufactured according to Comparative Example 1, and satisfactory results were obtained.

5. Measurement of transepidermal water loss and skin moisture content

Table 5 shows the results of measuring transepidermal water loss and skin moisture content after 3 weeks of use of cosmetics according to Manufacturing Examples 1 to 2 and Comparative Example 1. Transepidermal water loss (TEWL) was measured using Tewameter TM300 (C+K, Germany) after inducing skin barrier damage on the skin of the arm using tape stripping, and the temperature and humidity sensors of the probe measured the moisture evaporation amount (g/h㎡) over time per unit area of the measurement area. Skin moisture content was measured using Corneometer CM825 (C+K, Germany). The moisture content is expressed by utilizing the principle that the higher the moisture content of the skin, the more the electric capacitance of the conductor in the device increases. The measurement was performed three times for each test area, and the average value was analyzed.

The method for measuring transepidermal water loss (TEWL) and skin hydration (Capacitance, AU) was as follows: the measurement area was marked, TEWL and capacitance were measured before destroying the stratum corneum, 50 strippings were performed using 3M tape, and TEWL and capacitance were measured. For the experiment to measure transepidermal water loss and skin hydration, the transepidermal water loss and skin hydration were measured before using the cosmetics according to Manufacturing Examples 1 to 2 and Comparative Example 1 and after 3 weeks of use.

note Transepidermal water loss (average, %) Skin moisture content (average, %) Manufacturing example 1 6.12 54.92 Manufacturing example 2 7.84 51.32 Comparative Example 1 14.52 29.01

As shown in Table 5, the results of measuring transepidermal water loss and skin moisture content showed that when cosmetics manufactured according to Manufacturing Examples 1 and 2 were used, a decrease in transepidermal water loss and an increase in skin moisture content were observed compared to when cosmetics manufactured according to Comparative Example 1, indicating that skin condition was improved.

6. Itching Assessment

Itching was evaluated by using the Visual Analogue Scale (VAS), an itch evaluation tool, on a 10-point scale for the average and maximum itching for 24 hours at each visit. The subjects were asked to mark the degree of itching they felt on a 10 cm line, and the length of the line was measured. The length of the line marked by the subjects during the test period was compared to evaluate the improvement in itching according to Manufacturing Example 1 according to the criteria in Table 6. No itching was indicated as "0", and the worst itching imaginable was indicated as "10."

score Degree of itching 0 points No itching 1 point or more but less than 3 points mild pruritus 3 points or more but less than 7 points Moderate pruritus 7 points or more but less than 9 points severe pruritus 10 points very severe pruritus

As shown in Table 6, in the questionnaire evaluation on the usability of cosmetics according to Manufacturing Example 1, approximately 77 to 97% of the subjects responded positively to all items.

7. Primary irritation test on human skin

The IQ chamber, in which 20 μL of the cosmetic of Manufacturing Example 1 was dropped onto the test area, was applied as a closed patch to the test area for 24 hours. 30 minutes after removal of the patch, and 24 hours later, a first skin irritation test was performed based on the evaluation criteria of the International Contact Dermatitis Research Group (ICDRG) and the safety evaluation guidelines of the Personal Care Products Council (PCPC), and the degree of irritation was observed and classified as shown in Table 7.

- Primary Patch Test: The primary irritation test (patch safety evaluation), a type of skin safety evaluation, involves applying a test product to the skin for 24 or 48 hours and visually assessing the skin irritation and allergic reactions caused by the product by an expert to determine the degree of skin irritation.

- RIPT: Check whether the product induces skin sensitization and whether it induces sensitization, thereby determining the possibility of causing allergic contact dermatitis.

sign Score Skin reaction evaluation criteria Symptoms - 0 Negative reaction: no stimulation - ± 0.5 Doubtful or slight reaction: slight irritation Mild or barely perceptible erythema + 1 Weak(non-vesicular) positive response: light stimulation Erythema, edema and papules with distinct borders but mild ++ 2 Strong(vesicular) positive reaction: moderate stimulation Marked erythema, papules and vesicles +++ 3 Extreme positive reaction: strong irritation Severe erythema and vesicles, crusting

In Table 7, the average age of the 32 test subjects was 39.4 years old, and the selected test subjects had no specific skin symptoms and no history of diseases or medications that could affect the test.

The cosmetic product according to Manufacturing Example 1 was found to be a non-irritating product with a skin irritation index of 0.00 30 minutes and 24 hours after removal of the patch.

In addition, as a result of the skin irritation index assessment of the cosmetic according to Manufacturing Example 1, it was assessed as a non-irritating product with a skin irritation index of 0.00.

8. Evaluation of effectiveness for UV protection, wrinkle improvement and whitening functions

The effectiveness of the UV protection functionality, wrinkle improvement, and whitening functionality of the product according to Manufacturing Example 2 was evaluated. At this time, the efficacy of the product was confirmed through visual evaluation and instrumental evaluation as a test to evaluate the wrinkle improvement effect.

The effectiveness of functional and non-functional products for improving whitening was evaluated by measuring changes in skin pigmentation (melanin) after product use using a colorimeter and confirming the change by taking facial photographs using photography equipment.

The effectiveness evaluation of UV blocking functional products confirmed that the product has SPF50+PA++++ through the evaluation of UVA, UVB blocking effects and water resistance. SPF is a UV blocking value related only to UVB, and PA is a UV blocking value related only to UVA, and almost all sunscreens currently on the market in Korea have both of these functions. Most sunscreens are composite sunscreens, and the physical sunscreen raw materials have been nano-ized, and the uniformity of particles has been improved through improved powder processing and coating technology. The key to the technology is to manufacture a high-blocking sunscreen with almost no white cast.

9. Manufacturing of roller-type cosmetic containers with mirror function added by chrome deposition

Container lid manufacturing

A mixed resin was prepared by mixing polylactic acid, carrageenan, and alginic acid in a weight ratio of 1:0.28:0.07.

Next, with respect to 100 parts by weight of the mixed resin, 29 parts by weight of a thermoplastic elastomer, styrene-(ethylene-propylene)-styrene, 8.7 parts by weight of a cellulose fine powder having an average particle size of 5.5 μm, 3.2 parts by weight of montmorillonite having a particle size of 20.0 μm, 1.2 parts by weight of an internal lubricant, 0.76 parts by weight of a plasticizer, 1.8 parts by weight of a heat stabilizer, 1.5 parts by weight of an antioxidant, 1.4 parts by weight of a light stabilizer, 0.62 parts by weight of a pigment, and 0.32 parts by weight of a dye were mixed to prepare a biodegradable polymer mixed resin.

The manufactured biodegradable polymer blend resin had a melt flow index of 0.65 g/10 min measured at 230°C and a load of 2.16 kg according to ASTM-D1238.

Next, a biodegradable polymer blend resin was injected into a pressure mold and molded to manufacture a container lid.

Manufacturing of roller-type cosmetic containers and mirror-type container lids

ABS was used as the injection molding material, and a cosmetic container was injection molded. Then, the manufactured cosmetic container was washed using normal hexane (detergent), heat dried, and then laser marking was performed to express various patterns or phrases on the surface of the dried container.

Next, the brush was rotated to contact and remove static electricity and foreign substances from each cosmetic container and container lid, and compressed air was sprayed onto the container to remove foreign substances such as dust.

Next, before forming a character printing layer on each of the cosmetic containers and container lids from which foreign substances were removed, the containers were preheated to 50°C and transported at approximately 30 m/min to ensure smooth silk printing of the character printing layer.

Next, ink was silk-printed onto a portion of the upper surface of each of the cosmetic container and the container lid to form a character printing layer representing the logo, and then drying was performed at 70°C. At this time, the ink used included 30 parts by weight of a mixture containing 60 parts by weight of isoamyl alcohol, 30 parts by weight of a glycol derivative organic solvent, 30 parts by weight of methyl isobutyl ketone and methyl salicylate in a weight ratio of 1:0.7, and 20 parts by weight of a pigment, relative to 100 parts by weight of polyurethane resin.

Next, UV coating was applied to the upper surface of each silk-printed cosmetic container and container lid, and UV drying was performed.

Next, the cosmetic containers and container lids that had undergone UV drying in a high vacuum were placed into the high vacuum furnace, and after chromium was added as metal powder, a current was passed through the heater to heat-treat them to form a chromium deposition layer. This process is a surface treatment method that uses metal powder to apply to molds and parts that require high-temperature oxidation resistance and high-temperature corrosion resistance. The product was placed into a mixed powder containing metal powder (aluminum, chromium, silicon) and an activator and a sintering inhibitor at a certain ratio, and diffusion heat treatment was performed in an inert atmosphere.

Next, in order to prevent damage to the chrome deposition layer of each of the cosmetic container and the container lid and to ensure reliability, a UV coating solution mixed with phytoncide essential oil was applied to the upper surface of the chrome deposition layer, and heat-dried at 75°C to manufacture the cosmetic container and the container lid.

Next, an antibacterial coating agent was coated on the entire inside of the mirror-shaped container lid manufactured by forming a chrome deposition layer on the outside of the container lid to form an antibacterial coating layer. At this time, the antibacterial coating agent was prepared by mixing 56.2 wt% of polylactic acid resin, 1.7 wt% of titanium dioxide, 0.8 wt% of ginger extract, 1.2 wt% of grapefruit seed extract, 8.1 wt% of tetraethoxysilane as a silane coupling agent, 1.6 wt% of hydroxyethyl cellulose as a thickener, and the remaining balance of 100 wt% of an isopropyl alcohol aqueous solution.

Although the embodiments and comparative examples of the present invention have been described in detail as above, the scope of the rights of the present invention is not limited thereto, and the scope of the rights of the present invention extends to a range substantially equivalent to the embodiments of the present invention.

S1: Water component heating stage
S2: Paid ingredient input and mixing stage
S3: High pressure emulsification stage
S4: Cosmetic Formation Stage

Claims (17)

A method for producing a peptide-ceramide containing nanoparticle micelle using a centrifugal microfluidic reactor,
The above centrifugal microfluidic reactor,
An injection unit having at least two microchannels through which a peptide solution and a ceramide solution are respectively injected;
A joint having a micro needle connecting the micro channels of the above injection part into one;
A cross-linking portion comprising a collection tube connected to the injection portion and wrapping and protecting the above joint; and
A protective cap coupled to the injection unit and the cross-linking unit to cover and protect the injection unit and the cross-linking unit;
A method for producing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor, characterized by including a. In the first paragraph,
The above centrifugal microfluidic reactor
A method for producing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor, characterized in that the reactor is mounted on a centrifuge and the rotational speed of the centrifuge is controlled in the range of 1,000 to 6,000 rpm. In the second paragraph,
A method for manufacturing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor, characterized in that the fluid A containing the peptide solution and the fluid B containing the ceramide solution are each injected into an injection unit having at least two microchannels, and then Janus microparticles in which two or more different components are implemented as a single particle are synthesized by controlling the diameter of the microneedle of the junction unit and controlling the centrifugal force. A nanoemulsion cosmetic composition manufactured by a method for manufacturing a peptide-ceramide-containing nanoparticle micelle using a centrifugal microfluidic reactor according to any one of claims 1 to 3. In paragraph 4,
The above nanoemulsion cosmetic composition
A nanoemulsion cosmetic composition comprising 0.8 to 20 wt% of the peptide-ceramide-containing nanoparticle micelle, 1.0 to 10.0 wt% of an ionic surfactant, 0.1 to 4.0 wt% of a fatty acid, 0.5 to 3.0 wt% of a stabilizer, 6 to 40 wt% of an oil component, 10 to 50 wt% of a skin physiologically active ingredient, and the remaining amount of a polyol. In paragraph 5,
The above peptide-ceramide containing nanoparticle micelles
A nanoemulsion cosmetic composition characterized by being a peptide-ceramide nanoparticle micelle containing 0.3 to 10.0 wt% of the above peptide and 0.5 to 10.0 wt% of ceramide. Step 1: heating an aqueous component including peptide-ceramide-containing nanoparticle micelles, ionic surfactants, stabilizers, fatty acids and polyols to 30 to 90°C;
Step 2: adding and mixing an oily component into the heated aqueous component to form a dispersion;
Step 3: homogenizing the above dispersion using a high-pressure emulsifier to form a nanoemulsion; and
Step 4 of forming a cosmetic composition including the above nanoemulsion;
A method for producing a nanoemulsion cosmetic composition, characterized by comprising: In Article 7,
In the above step 2,
A method for producing a nanoemulsion cosmetic composition, characterized in that the oil component comprises an oil component and a skin physiologically active component. There is Article 7,
The above nanoemulsion cosmetic composition
A method for manufacturing a nanoemulsion cosmetic composition characterized in that it is used in any one of sunscreen, lotion, essence and cushion cosmetics that have obtained triple functional cosmetics certification for whitening, wrinkle improvement and UV protection, certification for itchiness improvement efficacy by strengthening the skin barrier and certification for non-irritation of the skin. A container body containing a nanoemulsion cosmetic composition;
A contents discharge portion coupled to the above container body;
A roller coupled to a roller joint located above the contents discharge portion; and
Covering the above container body, and including a mirror-shaped container lid with chrome deposited thereon;
The above mirror-shaped container lid is a molded body composed of a biodegradable polymer blend resin;
An antibacterial coating layer formed on the entire inside of the above molded body; and
A chrome deposition layer formed over the entire exterior of the above molded body;
A roller-type cosmetic container characterized in that the nanoemulsion cosmetic composition is produced by a method for producing a nanoemulsion cosmetic composition according to any one of claims 7 to 9. In Article 10,
The above biodegradable polymer blend resin is,
A mixed resin comprising an aliphatic polyester resin, carrageenan and alginic acid;
thermoplastic elastomer;
Cellulose fine powder with an average particle size of 1.0 to 10.0 μm;
Filler having an average particle size of 0.1 to 50.0 μm; and
additive;
A roller-shaped cosmetic container characterized by including a . In Article 11,
The above aliphatic polyester resin contains polylactic acid,
The thermoplastic elastomer comprises at least one selected from styrene-(ethylene-propylene), styrene-(ethylene-butylene)-styrene, styrene-(ethylene-propylene)-styrene and styrene-(ethylene-butylene)-styrene-(ethylene-butylene).
The above filler comprises at least one selected from mica, talc and montmorillonite,
A roller-type cosmetic container, characterized in that the additive includes at least one selected from an internal activator, a plasticizer, a heat stabilizer, an antioxidant, a light stabilizer, a pigment, and a dye. In Article 11,
The above biodegradable polymer blend resin is,
A roller-type cosmetic container comprising 20 to 40 parts by weight of a thermoplastic elastomer, 5 to 15 parts by weight of a cellulose fine powder, 1 to 5 parts by weight of a filler, and 2 to 10 parts by weight of an additive, with respect to 100 parts by weight of the mixed resin, wherein the mixed resin comprises an aliphatic polyester resin, carrageenan, and alginic acid in a weight ratio of 1:0.2 to 0.4:0.05 to 0.15. In Article 10,
The above antibacterial coating layer
A roller-type cosmetic container formed with an antibacterial coating agent including a polylactic acid resin, titanium dioxide, ginger extract, grapefruit seed extract, a silane coupling agent, a thickener, and an isopropyl alcohol aqueous solution. In Article 14,
The above antibacterial coating agent,
A roller-type cosmetic container characterized by comprising 50 to 60 wt% of the polylactic acid resin, 1 to 3 wt% of titanium dioxide, 0.5 to 1.0 wt% of ginger extract, 0.5 to 1.5 wt% of grapefruit seed extract, 5 to 10 wt% of a silane coupling agent, 1 to 3 wt% of a thickener, and an isopropyl alcohol aqueous solution with the remaining balance among 100 wt%. Roller-type cosmetic container according to Article 10; and
Cosmetics containing a nanoemulsion cosmetic composition contained in the above roller-type cosmetic container;
A roller-type cosmetic product characterized by including: In Article 16,
The cosmetic containing the above nanoemulsion cosmetic composition is in a cream formulation.
A roller-type cosmetic product containing the above nanoemulsion paint composition is characterized by having a viscosity of 5,000 to 60,000 cps at 25°C.