JPH01105157A - Long-sized matter of slide for biochemical analysis - Google Patents

Abstract

PURPOSE:To facilitate loading and taking out of plural pieces of slides for biochemical analysis by connecting and forming said slides in one direction by a soft film and feeding the slide by each one piece to an analysis apparatus from a container in which said slides are collectively loaded. CONSTITUTION:The title long-sized matter 40 is constituted by providing, for example, ion selective electrode slides 30 to one face of a tape-like film 41. A freely flexible soft film-like material such as plastic film, paper or woven fabric is used for the film 41. The spacings between the adjacent slides 30 and the material and shape of the film 41 are adequately selected and the long- sized material 4 is folded and is housed in, for example, a container 61. The slides 3 are loaded by each one piece to the analysis region of the analysis apparatus at the time of an analysis operation. The loading and taking out are, therefore, easily executed without touching the slides 30.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a long slide for biochemical analysis. More specifically, the present invention relates to a long slide for biochemical analysis that has a large number of slides for biochemical analysis and is convenient to handle.

[Prior Art and its Problems] Conventionally, there has been a demand for simple and rapid quantitative analysis of various components contained in biological body fluids (whole blood, plasma, serum, urine, saliva, etc.).

In particular, in current medical care, importance is placed on clinical chemistry tests that perform quantitative analysis of specific components in biological fluids in order to perform accurate diagnosis and appropriate treatment. For this purpose, it is necessary to quickly conduct a large number of clinical chemistry tests, and medical institutions,
There is a need for a method and apparatus for easily, automatically, and continuously performing the above quantitative analysis in laboratories and the like.

For the above-mentioned quantitative analysis, biochemical analysis methods have been proposed that use, for example, multilayer analysis slides that use spectroscopic measurements and analysis slides that measure ion activity using ion-selective electrodes. , has been put into practice.

Quantitative analysis using the above-mentioned multilayer analysis slide (hereinafter sometimes referred to as "colorimetric analysis slide") involves spotting a small amount of the test liquid on the colorimetric analysis slide, and then holding it at a constant temperature for a predetermined period of time. Incubation), the target component in the test liquid and the reagent in the colorimetric analysis slide undergo a color reaction, and the color density is measured spectrophotometrically to quantitatively analyze the target component.

In addition, analysis slides using ion-selective electrodes (hereinafter referred to as
Quantitative analysis using an ion-selective electrode slide (sometimes referred to as an ion-selective electrode slide) involves applying a test solution and a reference solution to each of the electrically separated ion-selective layers of a sheet-shaped ion-selective electrode, and The ionic activity of the test liquid is measured by measuring the potential difference between the ion selective electrodes while the two liquids are electrically connected to each other.

When performing analysis using these analysis slides, you do not have to repeat the process of loading the analysis slides one by one into the analysis area (measurement area) of the analyzer and then removing the analysis slides from the analysis area after the analysis is completed. However, it was extremely complicated and required a long time for the analysis process. Also,
After the analysis is completed, the analysis slide is contaminated with the test liquid and may sometimes emit harmful gases, and if it is not handled properly, it may contaminate the environment or reduce the sensitivity of the analysis. Various problems arise.

Therefore, biochemistry analysis slides that can use such analysis slides as they are, handle a large number of analysis slides at once, and load the analysis slides one by one into the analysis area during analysis operations are It is requested.

[Object of the Invention] An object of the present invention is to provide a long slide for biochemical analysis suitable for automatic and continuous biochemical analysis.

Another object of the present invention is to provide a long slide that can house a large number of slides for biochemical analysis and load the slides one by one into the analysis area of an analyzer during analysis operations. It is.

Still another object of the present invention is to, without touching the slide,
It is an object of the present invention to provide a long slide for biochemical analysis that can be easily loaded into and removed from an analyzer.

[Summary of the Invention] The present invention resides in a long slide for biochemical analysis characterized by having a plurality of slides for biochemical analysis connected in one direction by a soft film.

[Detailed description of the invention] The invention will now be described with reference to the accompanying drawings.

An example of a biochemical analysis slide according to the present invention is a colorimetric analysis slide having a multilayer analysis element, FIG. 1 is an exploded perspective view showing one specific example thereof, and FIG. 2 is a specific example of the multilayer analysis element. FIG.

In FIG. 1, a colorimetric analysis slide 10 has a multilayer analysis element 11, an upper slide frame 13 having an opening 12 for liquid sample spotting, an opening 14 for accommodating the multilayer analysis element 11, and a multilayer analysis element 1°. 1, and a lower slide frame 17 having a reflection photometry opening 16. The upper slide frame 13, the spacer 15, and the lower slide frame 17 are made of paper, plastic, metal, or the like, and are bonded and fixed by adhesive, heat fusion, or other means. Also, spacer 15 and lower slide frame 1
7 may be integrally molded. The outer dimensions of the upper slide frame 13, the spacer 15, and the lower slide frame 17 are preferably rectangular, particularly square, with sides of about 20 to 30 mm in length.

In FIG. 2, the multilayer analytical element 11 has a structure in which a reagent layer 112 is laminated on a light-transmitting support 111, and a developing layer 113 is laminated thereon. In biochemical analysis, a test liquid is spotted on the spreading layer 113, the test liquid spreads in the spreading layer 113, and the target component moves to the reagent layer 112 and reacts with the reagent contained in the reagent layer 112. The change in color density developed by this process is measured by reflection photometry from the support 111 side, and the countercurrent component in the liquid to be tested is analyzed by the principle of colorimetry.

The multilayer analysis element 11 further includes a reflective layer, a light shielding layer, a filtration layer, and a registration layer as necessary.
Other layers known in the art may also be included, such as layers, water absorption layers, subbing layers, etc. Further, the developing layer and the reagent layer may be a single layer. The structure of the multilayer analytical element as shown in FIG. 2 is known per se, and in the present invention, the multilayer analytical element may have any structure.

For example, the support 111 is a film made of a material that is transparent to light and impermeable to water; examples of such materials include polyethylene terephthalate, polycarbonate of bisphenol A, polystyrene, and cellulose ester. Examples include polymers such as cellulose diacetate, cellulose triacetate, cellulose acetate propionate, etc. The thickness of the support 111 is approximately 50 μm to approximately 300 μm.
m, particularly preferably within the range of 80 to 200 μm, and its shape is approximately 10 to 20 mm x approximately 10 to 2
It is convenient that it is a 0 mm square.

The spreading layer 113 spreads the liquid to be inspected that is spot-supplied on its surface at a rate of approximately constant amount per unit area in the lateral (horizontal) direction without substantially unevenly distributing the components contained therein. It has a spreading effect. The expansion layer 113 is
It is composed of paper such as filter paper, woven fabrics, non-woven fabrics, etc. made from natural fibers, synthetic fibers, etc. Also, the deployment layer 1
13 may be made from a porous material consisting of particulate polymer.

The developing layer 113 further contains various types of hydrophilic polymers such as cellulose derivatives, polyvinylpyrrolidone, polyvinyl alcohol, and polyacrylamide, nonionic, cationic, anionic, and amphoteric surfactants in order to control the development of the test liquid. It can be impregnated with a surfactant, an appropriate buffer for stable analysis, and the like.

Reagent layer 112 contains a suitable reagent capable of reacting with the analyte to produce a colorimetrically detectable change in color density. The reagent layer 112 is preferably one in which one or more reagents are dispersed in a hydrophilic colloid such as gelatin, gelatin derivative, polyvinyl alcohol, polyacrylamide, or polyvinylpyrrolidone that acts as a binder.

Another example of the biochemical analysis slide of the present invention is an ion selective electrode slide used for electrolyte measurement etc.
FIG. 3A is a perspective view showing a specific example thereof, and FIG. 3B is an exploded perspective view thereof.

3A and 3B, the ion selective electrode slide 30 includes a lower support frame 31, a porous liquid distribution member 32.3.
3. Water-impermeable member 34, ion-selective electrode pair 35, 36, 37 with ion-selective layer facing downward, and upper frame portion 38
It consists of The lower support frame 31 has a horizontal passage 3
11.312, air vent hole 313.314.315.3
16, notches 317 and 318 are provided.

Porous liquid distribution members 32.33 are placed within the horizontal passages 311.312 when assembling this ion-selective electrode slide 30. The water-impermeable member 34 includes a rising passage 341
．． 341', 341'', 342.342', 342
', descending passages 343, 344, air vent holes 345, 34
6.347.348, cutout portions 349.350 are provided.

The ion selection electrode pair 35 is a pair of ion selection electrodes 351.
352, the ion selection electrode pair 36 is comprised of a pair of ion selection electrodes 361.362, and the ion selection electrode pair 37 is comprised of a pair of ion selection electrodes 371.372. The upper part 38 has air vent holes 381, 382, 383, 384.
, liquid spot holes 385.386, porous bridges 387. A recessed portion 388 is provided.

In such an ion-selective electrode slide 30, for example, the ion-selective electrode pairs 35, 36, and 37 are
”, using a pair of ion-selective electrodes for CI-, a reference solution whose ion activity is known is placed in the liquid spotting hole 385, and a test liquid whose ion activity is unknown is placed in the liquid spotting hole 385. Hole 386
, the reference liquid passes through the descending passage 343 and permeates into the porous liquid distribution member 32, and enters the ascending passage 341, 341'.
, 341” to the ion selective electrode 351.36 ri, 3
71, the liquid to be inspected passes through the descending passage 344, permeates into the porous liquid distribution member 33, and enters the ascending passage 3.
42. Ion selection electrode 35 via 342' and 342'
2.362.372 is reached. On the other hand, both liquids come into contact near the center of the porous bridge 387. As a result, between the ion selection electrodes 351 and 352, the ion selection electrode 36
Between 1 and 362, ion selection electrode 371 and 37
2 between the reference solution and the test solution, respectively.
Since a potential difference corresponding to the difference in the ion activities of K゛ and CI- is generated, the notch portion 317.318.349.350
By inserting a potential measuring probe (not shown) into the probe and bringing it into contact with each electrode to measure the potential difference, the ion activity in the test liquid can be measured based on a calibration curve determined in advance.

Regarding such ion-selective electrode slides, for example, Japanese Patent Application Laid-open No. 155961/1983 and Japanese Patent Application Laid-open No. 11/1982
This method is disclosed in detail in Japanese Patent No. 157, etc., and is known per se, and can be appropriately selected and adopted in the present invention.

FIG. 4 is a sectional view partially showing an embodiment of a long slide for biochemical analysis of the present invention, FIG. 4A is a top view thereof, and FIG. 4B is a bottom view thereof.

In FIG. 4, FIG. 4A, and FIG. 4B, a long biochemical analysis slide 40 is constructed by providing an ion-selective electrode slide 30 on one side of a tape-like film 41. The tape-like film 41 is narrower than the width of the ion-selective electrode slide 30, and the potential measurement probe (
(not shown) is exposed. The distance S between adjacent slides 30 may be zero so that both slides are brought into close contact with each other, and the thickness t of the slides 30 may be adjusted to allow the long slide object 40 to be freely folded and stored in a suitable container. The length may be twice or more. Furthermore, when storing the slides 30 in a container in the form shown in FIG. 9, it is necessary to make the interval S greater than or equal to the sum of the length of the slides 30 in the connection direction and the thickness t. It is. The interval S can be arbitrarily determined depending on the storage method of the slide long object 40.

The tape-like film 41 is a flexible, flexible film-like material such as a plastic film, paper, woven fabric, or non-woven fabric. When using the colorimetric analysis slide 10 as shown in FIG. Good too. When using the colorimetric analysis slide 10, the tape-like film 41 must be made of a light-transmitting material.

Further, the tape-like film 41 may not be continuous, but may connect only the ends of adjacent slides 30. In that case, even if the slide is a colorimetric analysis slide, the film 41 may not be continuous. It is not necessary to use a light-transmitting material.

FIG. 5 is a sectional view partially showing another embodiment of the long slide for biochemical analysis of the present invention.

In FIG. 5, a long slide 50 for biochemical analysis is
Biochemical analysis slides 30a and slides 30b that are adjacent to each other are connected surface to surface only at their ends by a film 51a, and slides 30b that are adjacent to each other are
and the slide 30c are connected by the film 51b only at the end portions of the back surfaces thereof, and the connected portions are connected alternately between the front surfaces and the back surfaces.

The spacing between adjacent slides 30, the material and shape of the film 51, etc. are the same as described in FIG. 4. However, the width of the film 51 is not particularly limited.

FIGS. 6 to 9 are schematic diagrams showing specific examples of the state in which the long slide for biochemical analysis of the present invention is stored in a suitable container or a slide housing section of an analyzer.

FIG. 6 shows the slide long object 40 shown in FIG.
1 shows the state when it is folded and stored. In FIG. 6, in order to store the long slide object 40 compactly, it is preferable that the distance between adjacent slides be at least twice the thickness of the slides. The container 61 is made of a suitable material such as plastic, and can be attached to the analyzer with the elongated slide object 40 stored therein. In addition, the container 61
may be a slide accommodating section provided in the analyzer, in which case the elongated slide object 40 may be mounted in the slide accommodating section.

FIG. 7 shows a state in which the elongated slide object 40 shown in FIG. 4 is wound and stored around a winding shaft 62 having a regular octagonal cross section. In FIG. 7, the slides are constructed so that they overlap by appropriately changing the spacing between adjacent slides. By doing this, even if the long slide object 40 is stored in a wound state for a long period of time, the slide will not be deformed.

The cross section of the winding 462 may be any other polygonal or circular shape. In particular, if it is circular, the interval between adjacent slides may be zero, or it may be of any fixed length.

FIG. 8 shows the slide long object 50 shown in FIG.
3 shows the state in which it is folded and stored. In FIG. 8, the long slide object 50 is constructed as shown in FIG. They can be located in the same plane. The container 63 may be a separate container similar to the container 61 in FIG. 6, or may be a slide accommodating portion provided in the analyzer.

FIG. 9 shows the slide elongated object 40 shown in FIG. 4,
A state in which the distance S between adjacent slides is greater than or equal to the sum of the length of the slides 30 in the connection direction and the thickness t is shown folded and stored in the container 64. 9th
In the figure, each slide has a weight load of other slides,
Since it is not subjected to other mechanical loads, it will not deform during storage. The container 64 may be independent as described above, or may be provided in the analyzer.

[Effects of the Invention] The long slide for biochemical analysis of the present invention can store a large number of slides for biochemical analysis at once, and during analysis operations, it is possible to load the slides one by one into the analysis area of an analyzer. It has the remarkable advantage of being suitable for automatic and continuous biochemical analysis.

The long slide for biochemical analysis of the present invention has a remarkable effect in that it can be easily loaded into and removed from an analyzer without touching the slide.

[Brief explanation of the drawing]

FIG. 1 is an exploded perspective view showing a colorimetric analysis slide having a multilayer analysis element. FIG. 2 is a perspective view showing a specific example of a multilayer analysis element. FIG. 3A is a specific example of an ion-selective electrode slide. FIG. 3B is an exploded perspective view thereof, FIG. 4 is a cross-sectional view partially showing an embodiment of the long slide for biochemical analysis of the present invention, and FIG. 4A is a perspective view showing the following: 4B is a bottom view thereof; FIG. 5 is a sectional view partially showing another embodiment of the long slide for biochemical analysis of the present invention; FIGS. 6 and 7; FIG. 8 and FIG. 9 are schematic diagrams showing a specific example of the state in which the long slide for biochemical analysis of the present invention is stored in a suitable container or a slide housing section of an analyzer. 10: Colorimetric analysis slide 11: Multilayer analysis element 12: Liquid sample spotting opening 13: Upper slide frame 14: Opening 15 spacer 16: Reflection photometry opening 17: Lower slide frame 111: Light-transmitting support 112: Reagent layer 113: Spreading layer 30: Ion selective electrode slide 31: Lower support frame 32.33: Porous liquid distribution surface material 34: Water impermeable member 35.36.37: Ion selective electrode pair 38 two upper parts Frame 40.50 Varnished long object 41.51a, 51b = Film 61.63.64: Container 62: Winding shaft

Claims (1)

[Claims] 1. 1. A long slide for biochemical analysis, characterized in that a plurality of slides for biochemical analysis are connected in one direction with a soft film. 2. Claim 1, wherein the slide for biochemical analysis is an analysis slide having a multilayer analysis element including at least a developing layer, a reagent layer, and a light-transmitting support.
Long slides for biochemical analysis as described in Section 1. 3. The long slide for biochemical analysis according to claim 1, wherein the slide for biochemical analysis is an analysis slide that includes at least an ion-selective electrode. 4. The slide for biochemical analysis according to claim 1, wherein the soft film is a tape-like film, and the slide for biochemical analysis is provided only on one side of the film. Long objects. 5. 2. The elongated slide for biochemical analysis according to claim 1, wherein the slides for biochemical analysis that are adjacent to each other are connected by the film only at their ends. 6. The slides for biochemical analysis that are adjacent to each other are connected by the film only at the ends of the slides, and the connected portions are alternate between the front surfaces and the back surfaces. The long slide for biochemical analysis according to claim 5, characterized in that the slide is connected. 7. 2. The long slide for biochemical analysis according to claim 1, wherein the slides for biochemical analysis are provided at intervals of at least twice the thickness of the slide. 8. The slide for biochemical analysis according to claim 7, wherein the slide for biochemical analysis is provided at intervals equal to or longer than the sum of the length in the connecting direction and the thickness of the slide. Long objects.