JP4071837B2 - Reagent composition for detecting ascorbic acid and test piece - Google Patents
Reagent composition for detecting ascorbic acid and test piece Download PDFInfo
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- JP4071837B2 JP4071837B2 JP20635496A JP20635496A JP4071837B2 JP 4071837 B2 JP4071837 B2 JP 4071837B2 JP 20635496 A JP20635496 A JP 20635496A JP 20635496 A JP20635496 A JP 20635496A JP 4071837 B2 JP4071837 B2 JP 4071837B2
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- ascorbic acid
- iron
- composition
- iii
- reagent
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title claims description 48
- 229960005070 ascorbic acid Drugs 0.000 title claims description 24
- 235000010323 ascorbic acid Nutrition 0.000 title claims description 24
- 239000011668 ascorbic acid Substances 0.000 title claims description 24
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 19
- 239000000203 mixture Substances 0.000 title claims description 16
- 238000012360 testing method Methods 0.000 title claims description 12
- 239000000872 buffer Substances 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 9
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 8
- -1 iron (III) compound Chemical class 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000007800 oxidant agent Substances 0.000 claims description 5
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 4
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 claims description 3
- ICIWUVCWSCSTAQ-UHFFFAOYSA-M iodate Chemical group [O-]I(=O)=O ICIWUVCWSCSTAQ-UHFFFAOYSA-M 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- LKZLBQPNDYMRJE-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;iron;sodium Chemical compound [Na].[Fe].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O LKZLBQPNDYMRJE-UHFFFAOYSA-N 0.000 claims description 2
- WPTCSQBWLUUYDV-UHFFFAOYSA-N 2-quinolin-2-ylquinoline Chemical compound C1=CC=CC2=NC(C3=NC4=CC=CC=C4C=C3)=CC=C21 WPTCSQBWLUUYDV-UHFFFAOYSA-N 0.000 claims description 2
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 2
- XSYOZMCONWIORT-UHFFFAOYSA-K azanium;iron(3+);oxalate;hydrate Chemical compound [NH4+].O.[Fe+3].[O-]C(=O)C([O-])=O.[O-]C(=O)C([O-])=O XSYOZMCONWIORT-UHFFFAOYSA-K 0.000 claims description 2
- 239000013522 chelant Substances 0.000 claims description 2
- 150000008040 ionic compounds Chemical class 0.000 claims description 2
- ZUEYPVKINKUKCB-UHFFFAOYSA-K azanium iron(3+) disulfate hydrate Chemical compound O.[NH4+].S(=O)(=O)([O-])[O-].[Fe+3].S(=O)(=O)([O-])[O-] ZUEYPVKINKUKCB-UHFFFAOYSA-K 0.000 claims 1
- 239000000523 sample Substances 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000007864 aqueous solution Substances 0.000 description 5
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 4
- 238000005470 impregnation Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- NALMPLUMOWIVJC-UHFFFAOYSA-N n,n,4-trimethylbenzeneamine oxide Chemical group CC1=CC=C([N+](C)(C)[O-])C=C1 NALMPLUMOWIVJC-UHFFFAOYSA-N 0.000 description 4
- 229920006316 polyvinylpyrrolidine Polymers 0.000 description 4
- 239000001509 sodium citrate Substances 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- MKWYFZFMAMBPQK-UHFFFAOYSA-J sodium feredetate Chemical compound [Na+].[Fe+3].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O MKWYFZFMAMBPQK-UHFFFAOYSA-J 0.000 description 4
- 239000011697 sodium iodate Substances 0.000 description 4
- 235000015281 sodium iodate Nutrition 0.000 description 4
- 229940032753 sodium iodate Drugs 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical compound N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000006172 buffering agent Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- BCHZICNRHXRCHY-UHFFFAOYSA-N 2h-oxazine Chemical compound N1OC=CC=C1 BCHZICNRHXRCHY-UHFFFAOYSA-N 0.000 description 1
- 229920002799 BoPET Polymers 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-L Malonate Chemical compound [O-]C(=O)CC([O-])=O OFOBLEOULBTSOW-UHFFFAOYSA-L 0.000 description 1
- ZYODDGMLUYKFJV-UHFFFAOYSA-N N.O.[Fe+3] Chemical compound N.O.[Fe+3] ZYODDGMLUYKFJV-UHFFFAOYSA-N 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 238000009535 clinical urine test Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 1
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 1
- 239000001013 indophenol dye Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 description 1
- 229920002114 octoxynol-9 Polymers 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 description 1
- 229920000259 polyoxyethylene lauryl ether Polymers 0.000 description 1
- 239000001818 polyoxyethylene sorbitan monostearate Substances 0.000 description 1
- 235000010989 polyoxyethylene sorbitan monostearate Nutrition 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- JLKDVMWYMMLWTI-UHFFFAOYSA-M potassium iodate Chemical compound [K+].[O-]I(=O)=O JLKDVMWYMMLWTI-UHFFFAOYSA-M 0.000 description 1
- 239000001230 potassium iodate Substances 0.000 description 1
- 235000006666 potassium iodate Nutrition 0.000 description 1
- 229940093930 potassium iodate Drugs 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【０００１】
【発明の属する技術分野】
本発明は、液体試料中の、さらに詳しくは、全血、血清、血漿、尿、髄液、唾液などの生体試料中のアスコルビン酸を検出するための試薬組成物及び試験片に関する。
【０００２】
【従来の技術】
液体試料中のアスコルビン酸を検出するための方法は、種々存在し、知られている。
例えば、特開昭５５−１４２２４９号には、キレート剤と鉄（III）イオン（又は銅（II）イオン）との錯体、金属指示薬、緩衝剤などを用いた方法が示されている。
また、特開昭５７−１５４０５６号、特開昭５８−６３８５０号、特開昭６０−１６２９５４号、特開平１−２５９２６０号には、インドフェノール系色素を用いた方法が示されている。
また、特開昭５４−８０７９３号、特開昭５９−１０６４７６号、特開平１−２１３５７４号には、テトラゾリウム系色素を用いた方法が示されている。
また、特開昭５４−３０８９０号には、オキサジン系色素を用いた方法が示されている。
また、特開昭４８−１００１８７号、特開昭５６−８５５０号には、モリブデン酸を用いた方法が示されている。
また、特開昭６０−６８７０号には、銅イオンを用いて、アスコルビン酸が酸化されて出る過酸化水素による検出を用いた方法が示されている。
【０００３】
【発明が解決しようとする課題】
最近の尿試験片、例えばグルコース、潜血などの項目においては、尿中のアスコルビン酸等還元性物質の影響をある程度の濃度までは、回避できるように工夫されてきているが、充分なものではなく、還元性物質を検出するために、測定レンジが広い試験片が必要となってきている。
しかし、上記の従来技術では、１００ｍｇ／ｄｌ程度までのアスコルビン酸しか検出できないという課題があった。
【０００４】
【課題を解決するための手段】
本発明は、上記課題を解決するために、液体試料中のアスコルビン酸を検出するための試薬組成物において、
（ｉ）鉄（III）化合物、
（ii）鉄（II）イオン検出試薬、
（iii）酸化剤
を含み、かつ
酸化剤がヨウ素酸塩であることを特徴とするアスコルビン酸検出用組成物である。
また、液体試料中のアスコルビン酸を検出するための試験片において、上記の試薬組成物を保持体に保持することを特徴とする試験片である。
【０００５】
【発明の実施の形態】
本発明は、さらに、緩衝剤を含むことが好ましい。
緩衝剤は、リン酸緩衝液、酢酸緩衝液、マロン酸緩衝液、クエン酸緩衝液など種々用いることができる。
また、緩衝剤のｐＨは、２〜７であることが好ましい。
【０００６】
さらに、本発明には、ポリマーや界面活性剤を含むことができる。
ポリマーは、ヒドロキシプロピルセルロース、ポリビニルピロリドン、ポリビニルアルコール、アルギン酸ナトリウム、ポリアクリル酸ナトリウム、ゼラチンなど種々用いることができる。
界面活性剤は、ポリオキシエチレンオクチルフェニルエーテル（トリトンＸ−１００）、ポリオキシエチレンソルビタンモノラウレート（ツイーン２０）、ポリオキシエチレンソルビタンモノステアレート（ツイーン６０）、ポリオキシエチレンラウリルエーテル（ブリッジ３５）、ポリオキシエチレンセチルエーテル（ブリッジ５８）など種々用いることができる。
【０００７】
本発明における鉄（ＩＩＩ）化合物は、エチレンジアミン四酢酸一ナトリウム鉄（ＩＩＩ）、シュウ酸アンモニュウム鉄（ＩＩＩ）水和物、硫酸鉄（ＩＩＩ）アンモニュウム水和物、塩化鉄（ＩＩＩ）等のキレート化合物やイオン化合物から選択することができる。
【０００８】
また、鉄（ＩＩ）イオン検出試薬は、２，２’−ビピリジン、２，２’−ビキノリン、１，１０−フェナントトリン等の化合物や誘導体から選択することができる。
【０００９】
また、酸化剤は、ヨウ素酸ナトリウム、ヨウ素酸カリウムなどのヨウ素酸塩であるが、ヨウ素酸ナトリウムが好ましい。
本発明は、さらに、液体試料中のアスコルビン酸を検出するための液状試薬や錠剤試薬に応用することも可能である。
以下に実施例を示すが、本発明はこれに限定されるものではない。
【００１０】
【実施例１】
濾紙（ワットマン社製；３ＭＭＣｈｒ）を、下記に示す試薬含浸液に含浸、乾燥した。
＜処方＞
２，２’−ビピリジン（ナカライテスク製） ３ ｇ
エチレンジアミン四酢酸鉄（ＩＩＩ）−ナトリウム ４ ｇ
（ナカライテスク製）
ヨウ素酸ナトリウム（ナカライテスク製） １ ｇ
ポリビニルピロリドンＫ−３０（ナカライテスク製） １０ ｇ
０．５Ｍクエン酸／クエン酸ナトリウム緩衝液（ｐＨ４） ８０ ｍｌ
エタノール ２０ ｍｌ
【００１１】
【実施例２】
濾紙（ワットマン社製；３ＭＭＣｈｒ）を、下記に示す試薬含浸液に含浸、乾燥した。
＜処方＞
１，１０−フェナントロリン（ナカライテスク製） ３ ｇ
エチレンジアミン四酢酸鉄（ＩＩＩ）−ナトリウム ４ ｇ
（ナカライテスク製）
ヨウ素酸ナトリウム（ナカライテスク製） １ ｇ
ポリビニルピロリドンＫ−３０（ナカライテスク製） １０ ｇ
０．５Ｍクエン酸／クエン酸ナトリウム緩衝液（ｐＨ４） ８０ ｍｌ
エタノール ２０ ｍｌ
【００１２】
【比較例１】
濾紙（ワットマン社製；３ＭＭＣｈｒ）を、下記に示す試薬含浸液に含浸、乾燥した。
＜処方＞
２，２’−ビピリジン（ナカライテスク製） ３ ｇ
エチレンジアミン四酢酸鉄（ＩＩＩ）一ナトリウム ４ ｇ
（ナカライテスク製）
ポリビニルピロリドンＫ−３０（ナカライテスク製） １０ ｇ
０．５Ｍクエン酸／クエン酸ナトリウム緩衝液（ｐＨ４） ８０ ｍｌ
エタノール ２０ ｍｌ
【００１３】
【比較例２】
濾紙（ワットマン社製；３ＭＭＣｈｒ）を、下記に示す試薬含浸液に含浸、乾燥した。
＜処方＞
１，１０−フェナントロリン（ナカライテスク製） ３ ｇ
エチレンジアミン四酢酸鉄（ＩＩＩ）一ナトリウム ４ ｇ
（ナカライテスク製）
ポリビニルピロリドンＫ−３０（ナカライテスク製） １０ ｇ
０．５Ｍクエン酸／クエン酸ナトリウム緩衝液（ｐＨ４） ８０ ｍｌ
エタノール ２０ ｍｌ
【００１４】
実施例１、２及び比較例１、２にて作製したこの片面に両面テープ（ソニーケミカル製；Ｔ４０００）を貼り、５ｍｍ×５ｍｍにカットし、試薬部分を得た。これを５ｍｍ×６０ｍｍのＰＥＴフィルムの先端部に貼付し、試験片を得た。
＜測定方法＞
実施例１、２及び比較例１、２にて作製した上記試験片の試薬部分に下記試料１〜６を７μｌ滴下して、各試験紙の最大吸収波長における１分後の反射率変化を、色差計（日本分光製；Σ−９０）を用いて、測定した。
試料１；蒸留水
試料２；アスコルビン酸水溶液 （ １０ｍｇ／ｌ）
試料３；アスコルビン酸水溶液 （ ３０ｍｇ／ｌ）
試料４；アスコルビン酸水溶液 （ ５０ｍｇ／ｌ）
試料５；アスコルビン酸水溶液 （１００ｍｇ／ｌ）
試料６；アスコルビン酸水溶液 （３００ｍｇ／ｌ）
ここで、最大吸収波長は、実施例１及び比較例１においては５４０ｎｍ、実施例２及び比較例２においては５１０ｎｍとなった。
＜結果＞
結果として、実施例と比較例の最大吸収波長における反射率変化をまとめ、表１に示す。
【００１５】
【表１】
しかも、試薬部分の発色は、発色系であるため肉眼比色においても有利である。
【００１６】
【発明の効果】
本発明によって、従来よりも広い測定レンジを持つ試験片を作製することが可能になった。[0001]
BACKGROUND OF THE INVENTION
The present invention, in a liquid sample, the detail is al, whole blood, serum, plasma, urine, spinal fluid, a reagent composition and a test strip for detecting ascorbic acid in biological samples such as saliva.
[0002]
[Prior art]
Method for detecting ascorbic acid in the liquid sample, various exist, are known.
For example, Japanese Patent Application Laid-Open No. 55-142249 discloses a method using a complex of a chelating agent and iron (III) ions (or copper (II) ions), a metal indicator, a buffering agent and the like.
JP-A-57-154056, JP-A-58-63850, JP-A-60-162955, and JP-A-1-259260 show methods using indophenol dyes.
JP-A-54-80793, JP-A-59-106476, and JP-A-1-213574 disclose a method using a tetrazolium dye.
JP-A-54-30890 discloses a method using an oxazine dye.
Japanese Patent Application Laid-Open Nos. 48-1000018 and 56-8550 disclose methods using molybdic acid.
Japanese Patent Laid-Open No. 60-6870 discloses a method using detection with hydrogen peroxide which is produced by oxidizing ascorbic acid using copper ions.
[0003]
[Problems to be solved by the invention]
In recent urine test specimens such as glucose and occult blood, it has been devised to avoid the effects of reducing substances such as ascorbic acid in urine to a certain level, but it is not sufficient. In order to detect a reducing substance, a test piece having a wide measurement range is required.
However, the above conventional technique has a problem that only ascorbic acid up to about 100 mg / dl can be detected.
[0004]
[Means for Solving the Problems]
To solve the above problems, the present invention provides a reagent composition for detecting ascorbic acid in a liquid sample.
(I) an iron (III) compound,
(Ii) an iron (II) ion detection reagent,
(Iii) an oxidizing agent only contains, and
The composition for detecting ascorbic acid, wherein the oxidizing agent is iodate .
Further, in the test piece for detecting ascorbic acid in the liquid sample, the test composition is characterized in that the reagent composition is held on a holding body.
[0005]
DETAILED DESCRIPTION OF THE INVENTION
The present invention preferably further includes a buffer.
Various buffers such as a phosphate buffer, an acetate buffer, a malonate buffer, and a citrate buffer can be used.
Moreover, it is preferable that pH of a buffering agent is 2-7.
[0006]
Further, the present invention can include a polymer and a surfactant.
Various polymers such as hydroxypropyl cellulose, polyvinyl pyrrolidone, polyvinyl alcohol, sodium alginate, sodium polyacrylate, gelatin and the like can be used.
Surfactants include polyoxyethylene octyl phenyl ether (Triton X-100), polyoxyethylene sorbitan monolaurate (Tween 20), polyoxyethylene sorbitan monostearate (Tween 60), polyoxyethylene lauryl ether (Bridge 35). ), Polyoxyethylene cetyl ether (bridge 58), and the like.
[0007]
The iron (III) compound in the present invention is a chelate compound such as ethylenediaminetetraacetic acid monosodium iron (III), ammonium oxalate iron (III) hydrate, iron (III) ammonium hydrate, iron (III) chloride, etc. Or an ionic compound.
[0008]
The iron (II) ion detection reagent can be selected from compounds and derivatives such as 2,2′-bipyridine, 2,2′-biquinoline, 1,10-phenanthrin.
[0009]
Further, the oxidizing agent is sodium iodate, although Ru iodate der, such as potassium iodate, sodium iodate is preferred.
The present invention can also be applied to liquid reagents and tablet reagents for detecting ascorbic acid in a liquid sample.
Examples are shown below, but the present invention is not limited thereto.
[0010]
[Example 1]
A filter paper (manufactured by Whatman; 3MMChr) was impregnated in the reagent impregnation solution shown below and dried.
<Prescription>
2,2'-bipyridine (manufactured by Nacalai Tesque) 3 g
Ethylenediaminetetraacetic acid iron (III) -sodium 4 g
(Nacalai Tesque)
Sodium iodate (manufactured by Nacalai Tesque) 1 g
Polyvinylpyrrolidone K-30 (manufactured by Nacalai Tesque) 10 g
0.5 M citrate / sodium citrate buffer (pH 4) 80 ml
Ethanol 20 ml
[0011]
[Example 2]
A filter paper (manufactured by Whatman; 3MMChr) was impregnated in the reagent impregnation solution shown below and dried.
<Prescription>
1,10-phenanthroline (manufactured by Nacalai Tesque) 3 g
Ethylenediaminetetraacetic acid iron (III) -sodium 4 g
(Nacalai Tesque)
Sodium iodate (manufactured by Nacalai Tesque) 1 g
Polyvinylpyrrolidone K-30 (manufactured by Nacalai Tesque) 10 g
0.5 M citrate / sodium citrate buffer (pH 4) 80 ml
Ethanol 20 ml
[0012]
[Comparative Example 1]
A filter paper (manufactured by Whatman; 3MMChr) was impregnated in the reagent impregnation solution shown below and dried.
<Prescription>
2,2'-bipyridine (manufactured by Nacalai Tesque) 3 g
Ethylenediaminetetraacetic acid iron (III) monosodium 4 g
(Nacalai Tesque)
Polyvinylpyrrolidone K-30 (manufactured by Nacalai Tesque) 10 g
0.5 M citrate / sodium citrate buffer (pH 4) 80 ml
Ethanol 20 ml
[0013]
[Comparative Example 2]
A filter paper (manufactured by Whatman; 3MMChr) was impregnated in the reagent impregnation solution shown below and dried.
<Prescription>
1,10-phenanthroline (manufactured by Nacalai Tesque) 3 g
Ethylenediaminetetraacetic acid iron (III) monosodium 4 g
(Nacalai Tesque)
Polyvinylpyrrolidone K-30 (manufactured by Nacalai Tesque) 10 g
0.5 M citrate / sodium citrate buffer (pH 4) 80 ml
Ethanol 20 ml
[0014]
A double-sided tape (manufactured by Sony Chemicals; T4000) was applied to one side produced in Examples 1 and 2 and Comparative Examples 1 and 2, and cut into 5 mm × 5 mm to obtain a reagent portion. This was stuck on the front-end | tip part of 5 mm x 60 mm PET film, and the test piece was obtained.
<Measurement method>
7 μl of the following samples 1 to 6 were dropped on the reagent portions of the test pieces prepared in Examples 1 and 2 and Comparative Examples 1 and 2, and the reflectance change after 1 minute at the maximum absorption wavelength of each test paper was Measurement was performed using a color difference meter (manufactured by JASCO; Σ-90).
Sample 1; Distilled water sample 2; Ascorbic acid aqueous solution (10 mg / l)
Sample 3; ascorbic acid aqueous solution (30 mg / l)
Sample 4: Ascorbic acid aqueous solution (50 mg / l)
Sample 5: Ascorbic acid aqueous solution (100 mg / l)
Sample 6: Ascorbic acid aqueous solution (300 mg / l)
Here, the maximum absorption wavelength was 540 nm in Example 1 and Comparative Example 1, and 510 nm in Example 2 and Comparative Example 2.
<Result>
As a result, the change in reflectance at the maximum absorption wavelength in Examples and Comparative Examples is summarized and shown in Table 1.
[0015]
[Table 1]
In addition, since the coloring of the reagent portion is a coloring system, it is advantageous also in the naked eye colorimetry.
[0016]
【The invention's effect】
The present invention makes it possible to produce a test piece having a wider measurement range than before.
Claims (8)
（ｉ）鉄（III）化合物、
（ii）鉄（II）イオン検出試薬、
（iii）酸化剤
を含み、かつ
酸化剤がヨウ素酸塩であることを特徴とするアスコルビン酸検出用組成物。In a reagent composition for detecting ascorbic acid in a liquid sample,
(I) an iron (III) compound,
(Ii) an iron (II) ion detection reagent,
(Iii) An ascorbic acid detection composition comprising an oxidizing agent, and the oxidizing agent is an iodate.
塩化鉄（III）のキレート化合物又はイオン化合物から選ばれることを特徴とする請求項１に記載のアスコルビン酸検出用組成物。Iron (III) compound is ethylenediaminetetraacetic acid monosodium iron (III), ammonium oxalate iron (III) hydrate, iron (III) ammonium sulfate hydrate, and chelate or ionic compound of iron (III) chloride The composition for detecting ascorbic acid according to claim 1, wherein the composition is selected from.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20635496A JP4071837B2 (en) | 1996-07-02 | 1996-07-02 | Reagent composition for detecting ascorbic acid and test piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20635496A JP4071837B2 (en) | 1996-07-02 | 1996-07-02 | Reagent composition for detecting ascorbic acid and test piece |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH1019874A JPH1019874A (en) | 1998-01-23 |
| JP4071837B2 true JP4071837B2 (en) | 2008-04-02 |
Family
ID=16521933
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20635496A Expired - Fee Related JP4071837B2 (en) | 1996-07-02 | 1996-07-02 | Reagent composition for detecting ascorbic acid and test piece |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4071837B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5152130B2 (en) * | 2009-09-08 | 2013-02-27 | 住友金属鉱山株式会社 | Simple analysis method and inspection kit for iron contained in dust |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55142249A (en) * | 1979-04-24 | 1980-11-06 | Shionogi & Co Ltd | Reduction type ascorbic acid detecting composition and test piece |
| DE3012368C2 (en) * | 1980-03-29 | 1982-04-15 | Boehringer Mannheim Gmbh, 6800 Mannheim | Methods and diagnostic means for the detection of redox reactions |
| US5264348A (en) * | 1991-05-13 | 1993-11-23 | Miles Inc. | Ascorbate interference-resistant composition, device and method of assaying for predetermined analyte |
-
1996
- 1996-07-02 JP JP20635496A patent/JP4071837B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH1019874A (en) | 1998-01-23 |
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