JP2001145490A - Plasmid cloned icosapentaenoic acid biosynthesis genes and cyanobacteria producing icosapentaenoic acid - Google Patents

Abstract

(57) [Summary] (Modified) [Problem] A plasmid that efficiently expresses n-3 series highly unsaturated fatty acids such as eicosapentaenoic acid (EPA) in Gram-negative bacteria and introduces the plasmid into the n-3 series highly unsaturated fatty acid. Provision of cyanobacteria that efficiently express saturated fatty acids. Kind Code: A1 A plasmid obtained by cloning a gene group encoding a group of eicosapentaenoic acid biosynthetic enzymes encoded by six specific base sequences into a broad-range host vector and an eicosapentaenoic acid obtained by introducing the plasmid, Produced cyanobacteria. [Effects] n-3 series polyunsaturated fatty acids such as EPA are useful for medicines, foods, feeds, etc., and cyanobacteria as an organism used for substance production are also advantageous in terms of price and environment.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plasmid containing a group of EPA biosynthesis genes obtained from a bacterium producing icosapentaenoic acid (hereinafter referred to as EPA), and a trait for producing icosapentaenoic acid produced by introducing the plasmid. Related to transformed cyanobacteria.

[0002]

2. Description of the Related Art N-3 series unsaturated fatty acids such as icosapentaenoic acid and docosahexaenoic acid are constituent fatty acids of cell membranes and play an important role in the expression of normal functions of the circulatory system and the cerebral nervous system. Are known. However, humans cannot rely on biosynthesis of these, and thus rely mainly on their intake from seafood. There are also many ethnic groups who do not conventionally consume fish and shellfish. Therefore, n-3 series polyunsaturated fatty acids are widely sold as medicines and health foods for treatment and prevention of lifestyle-related diseases. further,
It is also used as an essential nutrient for foods for infants, such as powdered milk, and for cultured fry, and is added to feed.

At present, fish such as tuna and sardines are mainly used as raw materials for n-3 series polyunsaturated fatty acids. These fish accumulate what they take in as bait rather than biosynthesize polyunsaturated fatty acids themselves.
Known organisms that can biosynthesize n-3 series highly unsaturated fatty acids are marine microorganisms that are mainly low-order producers of the food chain in the sea, such as marine bacteria and marine microalgae,
No marine cyanobacteria producing this is known.

[0004] The marine cyanobacterium is n-
Takeyama et al., Microbiolgy (H. Takeyama et al., Who introduced the EPA biosynthetic genes derived from marine bacteria into Synechoccus sp. NKBG042902 as an example of introducing the biosynthetic genes of the three series of highly unsaturated fatty acids. , 143,2725-2731
(1997)). However, the introduced plasmid is about 50kb
Because of the huge thing, a problem occurred at the time of duplication and it was stable
No EPA production was seen.

[0005]

The marine cyanobacteria is important as a producer of oxygen on a global level, and can contribute to the reduction of carbon dioxide by utilizing its substance production capacity. Also, since cyanobacteria are autotrophic organisms by photosynthesis, it is considered that an inexpensive inorganic medium can be used for cultivation and no special equipment other than lighting is required, so that they can be easily put on an industrial level. Furthermore, since cyanobacteria are prokaryotes, it is also advantageous that the amount of waste can be kept low by using the whole cells as a raw material for producing substances. N-, a useful substance for cyanobacteria having such properties
The production of three series of unsaturated fatty acids is considered to be very significant. The present invention provides n-
The objective is to improve the ability to produce three-series highly unsaturated fatty acids, particularly EPA, and to stabilize the plasmid to be introduced.

[0006]

DISCLOSURE OF THE INVENTION The present invention provides a plasmid expressing polyunsaturated fatty acids in a wide range of bacteria, and a transformant cyanobacterium which stably and efficiently produces polyunsaturated fatty acids by introducing the plasmid. . That is, the present invention
Plasmids obtained by cloning the genes encoding the icosapentaenoic acid biosynthetic enzymes encoded by the nucleotide sequences represented by SEQ ID NOs: 2, 4, 6, 8, 10, and 12 into a broad-range host vector; , 7,
Genes encoding the icosapentaenoic acid biosynthetic enzymes represented by the base sequences represented by 9, 11, and 13
The present invention relates to a plasmid obtained by cloning into a broad-range host vector, and a cyanobacterium producing icosapentaenoic acid obtained by introducing these plasmids.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS EPA used in the present invention
Genes encoding biosynthetic enzymes are described in, for example, Example 1.
Alternatively, it can be isolated by the method described in JP-A-8-242867. In the present invention, EPA
The genes encoding the biosynthetic enzymes are SEQ.
Includes those that hybridize under stringent conditions with those encoded by the nucleotide sequences represented by 4, 6, 8, 10, and 12.

[0008] In order to introduce the genes encoding the EPA biosynthetic enzymes into other organisms, a vector having components for carrying and expressing these genes is required. When introducing the above-mentioned genes into prokaryotes, in many cases, the promoter / terminator can be used as it is, and therefore, a vector having a replication origin capable of replicating in each microorganism can be used. Common broad-range host vectors can be used, for example, pJRD215 (Davidson
et al., Gene, 51, 275-280 (1987)) and pBBR1MCS series (Kovach et al., Gene, 166, 175-176 (1995)). The cloning of the above-mentioned genes into these vectors is carried out by using DNA containing all the genes.
Using a fragment or a plurality of DNA fragments containing the entire gene group, it can be carried out in a conventional manner.

In general, methods for introducing a gene cloned into a vector into each prokaryote include a transformation method, a conjugation method, and an electroporation method. As a method for confirming the introduction of a gene into a cell, directly, a product resulting from the expression and a polyunsaturated fatty acid are detected. By performing organic solvent extraction from the organism into which the plasmid prepared as described above has been introduced,
Highly unsaturated fatty acids such as EPA can be obtained. Indirectly, it is possible to confirm that the gene has been introduced by hybridization or PCR using a part of the introduced gene as a probe or primer.

[0010] The above-described method can be applied to the introduction of the plasmid of the present invention into cyanobacteria. The cyanobacteria to be used is not particularly limited. For example, Synechococcus sp.NKBG15041c, Synechococcus sp.NKBG04
2902 and the like. As a cyanobacterium producing icosapentaenoic acid into which the above-described plasmid has been introduced,
15041c / pJRDEPA-S deposited with the National Institute of Advanced Industrial Science and Technology under the accession number FERM P-17634 on November 9, 2015.
Is mentioned.

[0011]

According to the present invention, the cells which are not essential for the biosynthesis of polyunsaturated fatty acids are shortened by removing as much as possible the portions which do not encode the ORF and the gene, so that when introduced into other organisms, Prevents dropout due to division and achieves stable and efficient expression of genes.

[0012]

Next, the present invention will be described more specifically with reference to examples and reference examples.

Example 1 Preparation of miniaturized plasmid EP inserted into plasmid pEPA described in JP-A-8-242867
ORF3, which is essential for EPA biosynthesis,
6, 7, 8 and 9 were subcloned. ORF5,
For 6, 7, 8 and 9, the cloning vector pBSI
XbaI-SpeI at the XbaI-SpeI site of IKS (+) (Stratagene)
Fragment (23,045-31,443), XbaI-XbaI fragment (1
2,314-23,045) SpeI-NheI fragment (31,443-32,5)
14) was sequentially subcloned to prepare ΔX4XbNh / pBS. ΔX4XbNh / pBS treated with NotI was blunt-ended with T4 DNA polymerase and treated with XhoI to obtain DNA fragment A. For ORF3, R / pSTV28
(HpaI fragment 7,951-9,129 was converted to Sma of Takara Shuzo vector pSTV28.
The fragment cut out after treating with EcoRI and PstI) was inserted into the EcoRI-PstI site of pBSIIKS (+) to
pBS was prepared. After treating R / pBS with PstI, blunt ends were created with T4 DNA polymerase and an XhoI linker was introduced, and then Xh
A fragment containing ORF3 was cut out with oI to obtain a DNA fragment B. Fragment A was introduced into the XhoI-StuI site of pJRD215 (kanamycin and streptomycin resistance), which is a broad-range host vector, using the Pacazine Lambda DNA packaging system (Promega), and fragment B was inserted into the XhoI site using a DNA ligation kit (Takara Shuzo). ) To complete the plasmid. This was named pJRDEPA-S (Figure 1).

Reference Example 1 Production of EPA by Escherichia coli Transfected with pJRDEPA-S Escherichia coli K12 / JM109 was transformed by a conventional method using pJRDEPA-S. LB agar medium containing 50 μg / ml kanamycin (1% tryptone, 0.5% yeast extract, 1% NaCl, 1.5% agar
%) To obtain a colony of JM109 / pJRDEPA-S. 2m containing 50μg / ml kanamycin
The cells were inoculated into an lLB liquid medium and cultured at 25 ° C for 24 hours. After the cells were collected by centrifugation to collect the cells and the medium was removed, 10 mM Tris-HCl buffer (pH 7.5) was added to suspend the cells, followed by centrifugation again for washing. A small amount of 10 mM Tris-HCl buffer (pH 7.5) was added to the washed cells, resuspended, and freeze-dried. To the dried cells, 1 ml of methanol containing 5% hydrogen chloride was added, and the mixture was treated at 80 ° C. for 1 hour to perform fatty acid methyl esterification. After cooling, the same amount of n-hexane was extracted three times, and the n-hexane layer was dried under reduced pressure and dissolved in 20 μl of methanol to obtain a sample. A part of this sample was analyzed by gas chromatography (hereinafter abbreviated as GLC). As a result, a peak was detected at a retention time similar to that of the standard EPA methyl ester. The ratio to the total fatty acids calculated from the area ratio of the peak was 5.7%. In addition, this peak has a gas chromatography mass spectrum (GC
The analysis showed that the parent ion (M) was m / z 316 and the base peak was m / z 79, which was consistent with the EPA methyl ester standard.

Example 2-1 Transconjugation of Cyanobacterium Cyanobacterium Synechococcus sp. NKBG15041c
(K. Sode et al., Appl. Microbiol. Biotechnol., 37,
369-373 (1992)) BG11 containing 3% NaCl (Table 1) liquid medium (BG11M) 1,000-1,500 by light irradiation under 23 ° C. of Lux performed 4-5 days static culture (A ₅₅₀ <1), The cells were collected by centrifugation at 3,000 rpm for 20 minutes at room temperature, suspended in BG11M liquid medium, and washed three times. The concentration of the cyanobacteria was adjusted to A ₅₅₀ = 1 with a spectrophotometer. Above p
E. coli S-17 for transconjugation with JRDEPA-S
(Simon et al., Bio / Technolgy, 118, 640-659 (1983))
Was cultured on an LB agar medium containing 50 μg / ml of kanamycin at 37 ° C. overnight, and the colonies grown were suspended in BG11M. The concentration was measured with a spectrophotometer, and adjusted to A ₆₅₀ = 10. The cyanobacterium and Escherichia coli prepared as described above were mixed in equal amounts so that Escherichia coli _A650 = 10 with _respect to the cyanobacteria _A550 = 1. After suspending this bacterial solution to 1/10 of the original after centrifugation, 50 μl of the suspension was added to a 1.2% agar medium obtained by adding 15 mM NaCl to BG11.
Each was spotted and cultured at 23 ° C. for 24 to 48 hours under light. Cut out the green colony formed on the agar medium with a scalpel and 1 m
The cells were suspended in lBG11M and added to a BG11M liquid medium containing 75 μg / ml kanamycin so as to have a concentration of 1/50, and cultured at 23 ° C. under illumination.

Table 1 Composition of BG11 ━━━━━━━━━━━━━━━━ NaNO ₃ 1.5 g MgSO ₄ .7H ₂ O 75 mg CaCl ₂ .2H ₂ O 36 mg Citric acid 6 mg Na ₂ EDTA 1 mg Na ₂ CO ₃ 20mg Trace metal mixture A5 * 1ml K ₂ HPO ₄ 30mg Iron ammonium citrate 6mg Vitamin B ₁₂ 1mg Deionized distilled water -1L pH 7.2-7.4 ━━━━━━━━━━━━━━━━ * trace metal mixture _{A5 ━━━━━━━━━━━━━━━ H 3 BO 4 2.86g MnCl} 2 · 6H 2 O 1.81g ZnSO 4 · 7H 2 O 222mg Na 2 MoO 4 · 2H 2 O 390mg _{CuSO 4 · 5H 2 O 79mg Co} (NO 3) 2 · 7H 2 O 49.4mg deionized distilled water -1 l ━━━━━━━━━━━━━━━

[0017] The cyanobacteria with a single colony isolation pJRDEPA-S of Example 2-2 cyanobacteria grown to A ₇₃₀ = 3-4, 10 ^-4 -
^10-5 dilution of BG11M containing 75 μg / ml kanamycin
And cultured for about 1 month under 23 ° C. illumination to form a single colony. This colony was transferred to a BG11M liquid medium containing 75 μg / ml kanamycin and cultured. The obtained recombinant cyanobacterium was named 15041c / pJRDEPA-S (Accession No. FERM P-17634).

Example 2-3 Preparation and Analysis of Cell Lipids of Cyanobacteria Cyanobacteria having pJRDEPA-S and non-recombinant cyanobacteria were cultured, and the cells were centrifuged in the same manner as in Example 1-2. After washing and freeze-drying. The dried cells were subjected to the same methyl esterification of fatty acids as in Example 1-2, extracted with n-hexane, dried under reduced pressure, and dissolved in methanol to obtain a crude sample. The crude sample was spotted on a silica gel thin layer plate and developed with n-hexane: ethyl ether (4: 1, v / v) to separate a methyl ester. The methyl ester fraction detected by primulin coloring was scraped off, and methanol: 10% saline (9: 1, v / v
v) From 1 ml of water and 1 ml of water, n-hexane extraction was performed in the same manner as above to prepare a sample. Part of this sample was GLC
And analyzed. A peak having the same retention time as that of the EPA methyl ester of the sample and a peak having almost the same height were detected about 1 minute before that. The ratio of the two peaks to the total fatty acids determined from the area ratio was 3.8% and 2.6%, respectively.
%Met. Table 2 shows the ratio of main fatty acids to total lipids. The remaining sample was spotted on a silver nitrate silica gel thin plate and n-hexane: ethyl ether (85:15, v / v)
Then, the polyunsaturated fatty acid fraction detected by 2 ', 7'-dichlorofluorescein coloring was scraped off, and a sample for GC-MS analysis was prepared in the same manner as described above. According to GC-MS analysis, the molecular weight of the peak having the same retention time as EPA methyl ester was 316, which was consistent with EPA. The previous peak had a molecular weight of 318 and a ratio of 20: 4, which was different from the retention time of arachidonic acid methyl ester (20: 4 (n-6)) by gas chromatography. This peak corresponds to the standard 20: 4 (n-
3) It was consistent with the retention time of methyl ester and the cleavage pattern of GC-MS, and was identified as 20: 4 (n-3) methyl ester.

Table 2 Main fatty acid composition of cyanobacteria introduced with pJRDEPA-S (% of total fatty acids)ラ ン Cyanobacteria introduced with fatty acid pJRDEPA-S Non-recombinant cyanobacterium ━━━━━━━━━━━━━━━━━━━━━━━━ ━━━━━━━━━━━━ 16: 0 32.6 33.1 16: 1 (n-7) 12.2 13.1 18: 1 (n-9) 12.8 19.1 18: 2 (n-6) 14.9 17.9 18: 3 (n-3) 6.7 7.1 20: 4 (n-3) 2.6 ND 20: 5 (n-3) 3.8 ND ━━━━━━━━━━━━━━━━━━━━━━━ ━━━━━━━━━━━━━ ND: Not detected

Example 3 Comparison of plasmids pJRDEPA-S and pJRDEPA (H. Takeyama et al., Micr
obiolgy, 143, 2725-2731 (1997)) was introduced into Synechococcus sp. NKBG15041c in the same manner as in Example 2-1 and cultured in 1 l of a BG11M liquid medium supplemented with 75 μg / ml kanamycin. A fatty acid methyl ester was prepared and analyzed by GLC in the same manner as in Example 2-3. The results are shown in Table 3.

Table 3 EPA-producing ability of NKBG15041C and introduced plasmid ━━━━━━━━━━━━━━━━━━━━━━━━━━━ Plasmid pJRDEPA-S pJRDEPA プ ラ ス ミ ド━━━━━━━━━━━━━━━━━━━━━━━ EPA (% *) 1.79 0.03 Size (Kb) 31 47 ━━━━━━━━━━━━━━ ━━━━━━━━━━━━━ *% of total fatty acids In NKBG15041c introduced with pJRDEPA-S, pJRDE
The EPA production capacity increased dramatically compared to the case where PA was introduced.

Reference Example 2 Cultivation Conditions for Cyanobacteria Cyanobacteria having pJRDEPA-S were cultured at normal temperature (23 ° C., 1,000 Lux, standing), at low temperature (18 ° C., 800 Lux, shaking) and under low light (23 ° C.).
(C, 40 Lux, standing)), and the fatty acid composition of each was analyzed by GLC in the same manner as in Example 2-3. Table 3 shows the results. The ratio of EPA and 20: 4 (n-3) to total fatty acids is higher when growth is suppressed (low temperature and low light) than normal, 6.0% compared to 3.8% and 2.6%, respectively.
・ 5.7%, 5.2% and 6.6%.

Table 4 Influence of culture conditions on main fatty acid composition (% of total fatty acids)脂肪酸 Fatty acid Low temperature Normal Low light ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 16: 0 37.4 32.6 36.4 16: 1 (n-7) 9.3 12.2 9.1 18: 1 (n-9) 8.3 12.8 7.5 18: 2 (n-6) 8.0 14.9 8.9 18: 3 (n-3) 4.8 6.7 2.6 20: 4 (n-3) 6.0 2.6 5.2 20: 5 ( n-3) 5.7 3.8 6.6 A ₇₃₀ 4.28 4.48 1.51 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Low temperature: 18 ℃, 800Lux, shake Normal: 23 ℃, 1,000lux, stationary Low light: 23 ℃, 40Lux, stationary

Reference Example 3 With respect to pJRDEPA, it has been reported that when the cyanobacterium was subcultured, the plasmid was lost in time for the replication of the cyanobacterium and the plasmid was lost (H. Takeyama et al., M.
icrobiolgy, 143,2725-2731 (1997)). But pJRDEPA
No such phenomenon was observed in the cyanobacteria having -S, and the passage could be repeated in the presence of kanamycin.

[0025]

[Sequence List] <110> Sagami Chemical Reserach Center, Japan Bioindustry Association, Director-General of Agency of Industrial Science and Technology. <120> A plasmid carrying the eicosapentanoic acid synthesis gene claster and a transgenic cyanobacterium producing eicosapentanoic acid. <130> SO18226 <160> 13

[0026] <210> 1 <211> 37895 <212> DNA <213> Shewanella putrefaciens SCRC-2874 (FERM BP-1625) <400>   1   Position   gatctcttac   aaagaaacta   tctcaatgtg   aatttaacct   taattccgtt   taattacggc   60   ctgatagagc   atcacccaat   cagccataaa   actgtaaagt   gggtactcaa   aggtggctgg   120   gcgattcttc   tcaaatacaa   agtgcccaac   ccaagcaaat   ccatatccga   taacaggtaa   180   aagtagcaat   aaaccccagc   gctgagttag   taatacataa   gcgaataata   ggatcactaa   240   actactgccg   aaatagtgta   atattcgaca   gtttctatgc   tgatgttgag   ataaataaaa   300   agggtaaaat   tcagcaaaag   aacgatagcg   cttactcatt   actcacacct   cggtaaaaaa   360   gcaactcgcc   attaacttgg   ccaatcgtca   gttgttctat   cgtctcaaag   ttatgccgac   420   taaataactc   tatatgtgca   ttatgattag   caaaaactcc   gataccatca   agatgaagtt   480   gttcatcaca   ccaactcaaa   actgcgtcga   taagcttact   gccatagccc   ttgccttgct   540   ccacatttgc   gatagcaata   aactgtaaaa   tgccacattg   gccacttggt   aagctctcta   600   taatctgatt   ttctttgtta   ataagtgcct   gagttgaata   ccaaccagta   cttaacaaca   660   tctttaaacg   ccaatgccaa   aaacgcgctt   cacctaaggg   aacctgctga   gtcactatgc   720   aggctacgcc   tatcaatcta   tccccaacga   acataccaat   aagtgcttgc   tcctgttgcc   780   agagctcatt   gagttcttct   cgaatagccc   cgcgaagctt   ttgctcatac   tgcgcttgat   840   caccactaaa   aagtgtttcg   ataaaaaagg   gatcatcatg   ataggcgtta   tagagaatag   900   aggctgctat   gcgtaaatct   tctgccgtga   gataaactgc   acgacactct   tccatggctt   960   gatcttccat   tgttattgtc   cttgaccttg   atcacacaac   accaatgtaa   caagactgta   1020   tagaagtgca   attaataatc   aattcgtgca   ttaagcaggt   cagcatttct   ttgctaaaca   1080   agctttattg   gctttgacaa   aactttgcct   agactttaac   gatagaaatc   ataatgaaag   1140   agaaaagcta   caacctagag   gggaataatc   aaacaactgc   taagatctag   ataatgtaat   1200   aaacaccgag   tttatcgacc   atacttagat   agagtcatag   caacgagaat   agttatggat   1260   acaacgccgc   aagatctatc   acacctgttt   ttacagctag   gattagcaaa   tgatcaaccc   1320   gcaattgaac   agtttatcaa   tgaccatcaa   ttagcggaca   atatattgct   acatcaagca   1380   agcttttgga   gcccatcgca   aaagcacttc   ttaattgagt   catttaatga   agatgcccag   1440   tggaccgaag   tcatcgacca   cttagacacc   ttattaagaa   aaaactaacc   attacaacag   1500   caactttaaa   ttttgccgta   agccatctcc   ccccacccca   caacagcgtt   gttgcttatg   1560   accactggag   tacattcgtc   tttagtcgtt   ttaccatcac   catgggtacg   ttgagtgcga   1620   taaaaaagca   cataaacttc   tttatcggcc   tgaatatagg   cttcgttaaa   atcagctgtt   1680   cccattaaag   taaccacttg   ctctttactc   atgcctagag   atatctttgt   caaattgtca   1740   cggtttttat   cttgagtttt   ctcccaagca   ccgtgattat   cccagtcaga   ttccccatca   1800   ccaacattga   ccacacagcc   cgttagccct   aagcttgcaa   tcccaaaaca   tgctaaacct   1860   aataatttat   ttttcatttt   aacttcctgt   tatgacatta   tttttgctta   gaagaaaagc   1920   aacttacatg   ccaaaacaca   agctgttgtt   ttaaatgact   ttatttatta   ttagcctttt   1980   aggatatgcc   tagagcaata   ataattacca   atgtttaagg   aatttgacta   actatgagtc   2040   cgattgagca   agtgctaaca   gctgctaaaa   aaatcaatga   acaaggtaga   gaaccaacat   2100   tagcattgat   taaaaccaaa   cttggtaata   gcatcccaat   gcgcgagtta   atccaaggtt   2160   tgcaacagtt   taagtctatg   agtgcagaag   aaagacaagc   aatacctagc   agcttagcaa   2220   cagcaaaaga   aactcaatat   ggtcaatcaa   gcttatctca   atctgaacaa   gctgatagga   2280   tcctccagct   agaaaacgcc   ctcaatgaat   taagaaacga   atttaatggg   ctaaaaagtc   2340   aatttgataa   cttacaacaa   aacctgatga   ataaagagcc   tgacaccaaa   tgcatgtaat   2400   tgaactacga   tttgaatgtt   ttgataacac   cacgattact   gcagcagaaa   aagccattaa   2460   tggtttgctt   gaagcttatc   gagccaatgg   ccaggttcta   ggtcgtgaat   ttgccgttgc   2520   atttaacgat   ggtgagttta   aagcacgcat   gttaacccca   gaaaaaagca   gcttatctaa   2580   acgctttaat   agtccttggg   taaatagtgc   actcgaagag   ctaaccgaag   ccaaattgct   2640   tgcgccacgt   gaaaagtata   ttggccaaga   tattaattct   gaagcatcta   gccaagacac   2700   accaagttgg   cagctacttt   acacaagtta   tgtgcacatg   tgctcaccac   taagaaatgg   2760   cgacaccttg   cagcctattc   cactgtatca   aattccagca   actgccaacg   gcgatcataa   2820   acgaatgatc   cgttggcaaa   cagaatggca   agcttgtgat   gaattgcaaa   tggccgcagc   2880   tactaaagct   gaatttgccg   cacttgaaga   gctaaccagt   catcagagtg   atctatttag   2940   gcgtggttgg   gacttacgtg   gcagagtcga   atacttgacg   aaaattccga   cctattacta   3000   tttataccgt   gttggcggtg   aaagcttagc   agtagaaaag   cagcgctctt   gtcctaagtg   3060   tggcagtcaa   gaatggctgc   tcgataaacc   attattggat   atgttccatt   ttcgctgtga   3120   cacctgccgc   atcgtatcta   atatctcttg   ggaccattta   taactcttcc   gagtcttatc   3180   acactagagt   ttagtcagca   taaaaatggc   gcttatattt   caattaaaag   aaatataagc   3240   gccattttca   tcgatactat   atatcagcag   actattttcc   gcgtaaatta   gcccacatta   3300   atttcattct   ttgccagatc   cctggatgat   ctagttgtgg   catcgactct   tcaataggtt   3360   taaccgcagg   tgtaaccctt   ggagtcaatt   cgtttataaa   ctcgtttaaa   ctgtcactta   3420   atttaacgct   ttgtacttca   cctggaattt   caatccatac   gctgccatca   ctattattaa   3480   ccgtcaacat   tttatcttca   tcatcaagaa   taccaataaa   ccaagtcggc   tcttgcttaa   3540   gctttctctt   catcattaaa   tgaccaatga   tgttttgttg   taagtattca   aaatcagttt   3600   gatcccacac   ttggattagc   tcaccttggc   cccattgtga   gtcaaaaaat   agcggtgcag   3660   aaaaatgact   gccaaaaaat   ggattaattt   ctgcagataa   tgtcatttca   agtgctgttt   3720   caacattagc   aaattcacca   ggttgttgac   gtacaaccga   ttgccaaaac   actgcgccat   3780   cggagcccgc   ttcggcgaca   acacactcag   acttttgtcc   ttgcgcataa   tatcttggct   3840   gttcaccaag   cttatccatg   taggcttgtt   gatatttaga   taaaaaaaga   tctaaagcag   3900   gtaaagaaga   cacttaagcc   agttccaaaa   tcagttataa   taggggtcta   ttttgacatg   3960   gaaaccgtat   tgatgacaca   acatcatgat   ccctacagta   acgcccccga   actttctgaa   4020   ttaactttag   gaaagtcgac   cggttatcaa   gagcagtatg   atgcatcttt   actacaagcg   4080   tgccgcgtaa   attaaaccgt   gatgctatcg   gtctaaccaa   tgagctacct   tttcatggct   4140   gtgatatttg   gactggctac   gaactgtctt   ggctaaatgc   taaaggcaag   ccaatgattg   4200   ctattgcaga   ctttaaccta   agttttgata   gtaaaaatct   gatcgagtct   aagtcgttta   4260   agctgtattt   aaacagctat   aaccaaacac   gatttgatag   cgttcaagcg   gttcaagaac   4320   gtttaactga   agacttaagc   gcctgtgccc   aaggcacagt   tacggtaaaa   gtgattgaac   4380   ctaagcaatt   taaccacctg   agagtggttg   atatgccagg   tacctgcatt   gacgatttag   4440   atattgaagt   tgatgactat   agctttaact   ctgactatct   caccgacagt   gttgatgaca   4500   aagtcatggt   tgctgaaacg   ctaacgtcaa   acttattgaa   atcaaactgc   ctaatcactt   4560   ctcagcctga   ctggggtaca   gtgatgatcc   gttatcaagg   gcctaagata   gaccgtgaaa   4620   agctacttag   atatctgatt   tcatttagac   agcacaatga   atttcatgag   cagtgtgttg   4680   agcgtatatt   tgttgattta   aagcactatt   gccaatgtgc   caaacttact   gtctatgcac   4740   gttatacccg   ccgtggtggt   ttagatatca   acccatatcg   tagcgacttt   gaaaaccctg   4800   cagaaaatca   gcgcctagcg   agacagtaat   tgattgcagt   acctacaaaa   aacaatgcct   4860   ataagccaag   cttatgggca   tttttatatt   atcaacttgt   catcaaacct   cagccgccaa   4920   gccttttagt   tttatcgcta   aattaagccg   ctctctcagc   caaatatttg   caggattttg   4980   ctgtaattta   tggctccaca   ccatgaaata   ctctatcggc   tctaccgcaa   aaggtaagtc   5040   aaatacctgt   aagccaaaca   gcttggcata   ttcgtcagtg   tgggcttttg   acgcgatagc   5100   taacgcatca   ctttttgagg   caaccgacat   catacttaat   attgatgatt   gctcgctgtg   5160   catttgcctt   gccggtaaca   cctgtttagt   cagcaagtcg   gcaacactta   aattgtagcg   5220   gcgcatctta   aaaataatat   gcttttcatt   aaagtattgc   tcttgcgtca   acccaccttg   5280   gatccttggg   tgagcatttc   gtgccacaca   aactaattta   tcctgcatta   ctttttgact   5340   cttaaatgcc   gcagattctg   gcagccaaat   atctaaggct   aaatccacct   tttctagttg   5400   taggtccatc   tgcaactctt   cttcaatgag   cggcggctca   cgaaatacaa   tattaattgc   5460   agtgccctgt   aacacttgct   caatttgatc   ttgcaagagt   tgtattgccg   actcgctggc   5520   atacacataa   aaagttcgct   cacttgaagt   ggggtcaaat   gcttcaaagc   tagtcgcaac   5580   ttgctcaatt   gttgacatag   cgcccgcgag   ctgttgataa   agcgtcatcg   cacttgcggt   5640   aggtttaact   cccctaccca   ctcgagtaaa   caactcttct   ccaacaatac   tttttagcct   5700   cgaaatcgca   ttactaaccg   acgactgagt   caaatccagc   tcttctgccg   cccggctaaa   5760   agatgaggtg   cgatacaccg   cagtaaaaac   gcgaaataaa   ttaagatcaa   aagctttttg   5820   ctgcgacata   aatcagctat   ctccttatcc   ttatccttat   ccttataaaa   agttagctcc   5880   agagcactct   agctcaaaaa   caactcagcg   tattaagcca   atattttggg   aactcaatta   5940   atattcataa   taaaagtatt   cataatataa   ataccaagtc   ataatttagc   cctaattatt   6000   aatcaattca   agttacctat   actggcctca   attaagcaaa   tgtctcatca   gtctccctgc   6060   aactaaatgc   aatattgaga   cataaagctt   tgaactgatt   caatcttacg   agggtaactt   6120   atgaaacaga   ctctaatggc   tatctcaatc   atgtcgcttt   tttcattcaa   tgcgctagca   6180   gcgcaacatg   aacatgacca   catcactgtt   gattacgaag   ggaaagccgc   aacagaacac   6240   accatagctc   acaaccaagc   tgtagctaaa   acacttaact   ttgccgacac   gcgtgcattt   6300   gagcaatcgt   ctaaaaatct   agtcgccaag   tttgataaag   caactgccga   tatattacgt   6360   gccgaatttg   cttttattag   cgatgaaatc   cctgactcgg   ttaacccgtc   tctctaccgt   6420   caggctcagc   ttaatatggt   gcctaatggt   ctgtataaag   tgagcgatgg   catttaccag   6480   gtccgcggta   ccgacttatc   taaccttaca   cttatccgca   gtgataacgg   ttggatagca   6540   tacgatgttt   tgttaaccaa   agaagcagca   aaagcctcac   tacaatttgc   gttaaagaat   6600   ctacctaaag   atggcgattt   acccgttgtt   gcgatgattt   actcccatag   ccatgcggac   6660   cactttggcg   gagctcgcgg   tgttcaagag   atgttccctg   atgtcaaagt   ctacggctca   6720   gataacatca   ctaaagaaat   tgtcgatgag   aacgtacttg   ccggtaacgc   catgagccgc   6780   cgcgcagctt   atcaatacgg   cgcaacactg   ggcaaacatg   accacggtat   tgttgatgct   6840   gcgctaggta   aaggtctatc   aaaaggtgaa   atcacttacg   tcgccccaga   ctacacctta   6900   aacagtgaag   gcaaatggga   aacgctgacg   attgatggtc   tagagatggt   gtttatggat   6960   gcctcgggca   ccgaagctga   gtcagaaatg   atcacttata   ttccctctaa   aaaagcgctc   7020   tggacggcgg   agcttaccta   tcaaggtatg   cacaacattt   atacgctgcg   cggcgctaaa   7080   gtacgtgatg   cgctcaagtg   gtcaaaagat   atcaacgaaa   tgatcaatgc   ctttggtcaa   7140   gatgtcgaag   tgctgtttgc   ctcgcactct   gcgccagtgt   ggggtaacca   agcgatcaac   7200   gatttcttac   gcctacagcg   tgataactac   ggcctagtgc   acaatcaaac   cttgagactt   7260   gccaacgatg   gtgtcggtat   acaagatatt   ggcgatgcga   ttcaagacac   gattccagag   7320   tctatctaca   agacgtggca   taccaatggt   taccacggca   cttatagcca   taacgctaaa   7380   gcggtttata   acaagtatct   aggctacttc   gatatgaacc   cagccaacct   taatccgctg   7440   ccaaccaagc   aagaatctgc   caagtttgtc   gaatacatgg   gcggcgcaga   tgccgcaatt   7500   aagcgcgcta   aagatgatta   cgctcaaggt   gaataccgct   ttgttgcaac   ggcattaaat   7560   aaggtggtga   tggccgagcc   agaaaatgac   tccgctcgtc   aattgctagc   cgatacctat   7620   gagcaacttg   gttatcaagc   agaaggggct   ggctggagaa   acatttactt   aactggcgca   7680   caagagctac   gagtaggtat   tcaagctggc   gcgcctaaaa   ccgcatcggc   agatgtcatc   7740   agtgaaatgg   acatgccgac   tctatttgac   ttcctcgcgg   tgaagattga   tagtcaacag   7800   gcggctaagc   acggcttagt   taagatgaat   gttatcaccc   ctgatactaa   agatattctc   7860   tatattgagc   taagcaacgg   taacttaagc   aacgcagtgg   tcgacaaaga   gcaagcagct   7920   gacgcaaacc   ttatggttaa   taaagctgac   gttaaccgca   tcttacttgg   ccaagtaacc   7980   ctaaaagcgt   tattagccag   cggcgatgcc   aagctcactg   gtgataaaac   ggcatttagt   8040   aaaatagccg   atagcatggt   cgagtttaca   cctgacttcg   aaatcgtacc   aacgcctgtt   8100   aaatgaggca   ttaatctcaa   caagtgcaag   ctagacataa   aaatggggcg   attagacgcc   8160   ccatttttta   tgcaattttg   aactagctag   tcttagctga   agctcgaaca   acagctttaa   8220   aattcacttc   ttctgctgca   atacttattt   gctgacactg   accaatactc   agtgcaaaac   8280   gataactatc   atcaagatgg   cccagtaaac   aatgccaatt   atcagcagcg   ttcatttgct   8340   gttctttagc   ctcaatcaaa   cctaaaccag   acttttgtgg   ctcagcgtta   ggcttattag   8400   aactcgactc   tagtaaagca   agaccaatat   cttgttttaa   caaaacctgt   cgctgattaa   8460   gttgatgctc   aaccttgtga   tccgcaatag   catcggaaat   atcaacacaa   tggctcaagc   8520   ttttaggtgc   attaactcca   agaaaagttt   cgctcagtgc   agagaagtca   aacgcaaaag   8580   attttagcga   taatgccagc   ccaagtcctt   tcgctttaat   gtaagactcc   ttgagcgccc   8640   acaaatcaaa   aaagcggtct   cgctgcaagg   cctctggtaa   cgctaacaag   gctcgctttt   8700   ctgattcaga   gaaataatga   ctaagaatag   agtggatatt   ggtgctgtta   cggcaacgct   8760   caatgtcgac   gccaaactca   atactagcag   agtcagtttc   ctccttgctt   gcctgactgg   8820   cgcctttatt   atcagcagtg   caaatgccta   ctaatagcca   atctccacta   tgactcacat   8880   taaagtggac   cccggtttga   gcaaattgcg   catcactcaa   tctaggctta   cctttgtcgc   8940   catattcaaa   gcgccattca   ttggggcgta   tttcactatg   ttgtgacaat   aaagcgcgca   9000   aatagcctct   taccattaaa   ccttgagttt   tagcttcttg   tttaatgtag   cgattaacct   9060   taattaactc   atcttcaggc   agccatgact   taaccaactc   tgtagtctgg   ttatcgcact   9120   cttgtattgt   taacggacag   aagtataagg   aaatcaatcg   agaagttagc   aatttttcag   9180   gacactcttt   aaagcaacaa   acataacccc   tatttttacc   aatttaagat   caaaactaaa   9240   gccaaaacta   attgagaata   gtgtcaaact   agctttaaag   gaaaaaaata   taaaaagaac   9300   attatacttg   tataaattat   tttacacacc   aaagccatga   tcttcacaaa   attagctccc   9360   tctccctaaa   acaagattga   ataaaaaaat   aaaccttaac   tttcatatag   ataaaacaaa   9420   ccaatgggat   aaagtatatt   gaattcattt   ttaaggaaaa   attcaaattg   aattcaagct   9480   cttcagtaaa   agcatatttt   gccgttagtg   tgaaaaaaaa   caaatttaaa   aaccaacata   9540   gaacaaataa   gcagacaata   aaaccaaggc   gcaacacaaa   caacgcgctt   acaattttca   9600   caaaaaagca   acaagagtaa   cgtttagtat   ttggatatgg   ttattgtaat   tgagaatttt   9660   ataacaatta   tattaaggga   atgagtatgt   ttttaaattc   aaaactttcg   cgctcagtca   9720   aacttgccat   atccgcaggc   ttaacagcct   cgctagctat   gcctgttttt   gcagaagaaa   9780   ctgctgctga   agaacaaata   gaaagagtcg   cagtgaccgg   atcgcgaatc   gctaaagcag   9840   agctaactca   accagctcca   gtcgtcagcc   tttcagccga   agaactgaca   aaatttggta   9900   atcaagattt   aggtagcgta   ctagcagaat   tacctgctat   tggtgcaacc   aacactatta   9960   ttggtaataa   caatagcaac   tcaagcgcag   gtgttagctc   agcagacttg   cgtcgtctag   10020   gtgctaacag   aaccttagta   ttagtcaacg   gtaagcgcta   cgttgccggc   caaccgggct   10080   cagctgaggt   agatttgtca   actataccaa   ctagcatgat   ctcgcgagtt   gagattgtaa   10140   ccggcggtgc   ttcagcaatt   tatggttcgg   acgctgtatc   aggtgttatc   aacgttatcc   10200   ttaaagaaga   ctttgaaggc   tttgagttta   acgcacgtac   tagcggttct   actgaaagtg   10260   taggcactca   agagcactct   tttgacattt   tgggtggtgc   aaacgttgca   gatggacgtg   10320   gtaatgtaac   cttctacgca   ggttatgaac   gtacaaaaga   agtcatggct   accgacattc   10380   gccaattcga   tgcttgggga   acaattaaaa   acgaagccga   tggtggtgaa   gatgatggta   10440   ttccagacag   actacgtgta   ccacgagttt   attctgaaat   gattaatgct   accggtgtta   10500   tcaatgcatt   tggtggtgga   attggtcgct   caacctttga   cagtaacggc   aatcctattg   10560   cacaacaaga   acgtgatggg   actaacagct   ttgcatttgg   ttcattccct   aatggctgtg   10620   acacatgttt   caacactgaa   gcatacgaaa   actatattcc   aggggtagaa   agaataaacg   10680   ttggctcatc   attcaacttt   gattttaccg   ataacattca   attttacact   gacttcagat   10740   atgtaaagtc   agatattcag   caacaatttc   agccttcatt   ccgttttggt   aacattaata   10800   tcaatgttga   agataacgcc   tttttgaatg   acgacttgcg   tcagcaaatg   ctcgatgcgg   10860   gtcaaaccaa   tgctagtttt   gccaagtttt   ttgatgaatt   aggaaatcgc   tcagcagaaa   10920   ataaacgcga   acttttccgt   tacgtaggtg   gctttaaagg   tggctttgat   attagcgaaa   10980   ccatatttga   ttacgacctt   tactatgttt   atggcgagac   taataaccgt   cgtaaaaccc   11040   ttaatgacct   aattcctgat   aactttgtcg   cagctgtcga   ctctgttatt   gatcctgata   11100   ctggcttagc   agcgtgtcgc   tcacaagtag   caagcgctca   aggcgatgac   tatacagatc   11160   ccgcgtctgt   aaatggtagc   gactgtgttg   cttataaccc   atttggcatg   ggtcaagctt   11220   cagcagaagc   ccgcgactgg   gtttctgctg   atgtgactcg   tgaagacaaa   ataactcaac   11280   aagtgattgg   tggtactctc   ggtaccgatt   ctgaagaact   atttgagctt   caaggtggtg   11340   caatcgctat   ggttgttggt   tttgaatacc   gtgaagaaac   gtctggttca   acaaccgatg   11400   aatttactaa   agcaggtttc   ttgacaagcg   ctgcaacgcc   agattcttat   ggcgaatacg   11460   acgtgactga   gtattttgtt   gaggtgaaca   tcccagtact   aaaagaatta   ccttttgcac   11520   atgagttgag   ctttgacggt   gcataccgta   atgctgatta   ctcacatgcc   ggtaagactg   11580   aagcatggaa   agctggtatg   ttctactcac   cattagagca   acttgcatta   cgtggtacgg   11640   taggtgaagc   agtacgagca   ccaaacattg   cagaagcctt   tagtccacgc   tctcctggtt   11700   ttggccgcgt   ttcagatcca   tgtgatgcag   ataacattaa   tgacgatccg   gatcgcgtgt   11760   caaactgtgc   agcattgggg   atccctccag   gattccaagc   taatgataac   gtcagtgtag   11820   ataccttatc   tggtggtaac   ccagatctaa   aacctgaaac   atcaacatcc   tttacaggtg   11880   gtcttgtttg   gacaccaacg   tttgctgaca   atctatcatt   cactgtcgat   tattatgata   11940   ttcaaattga   ggatgctatt   ttgtcagtag   ccacccagac   tgtggctgat   aactgtgttg   12000   actcaactgg   cggacctgac   accgacttct   gtagtcaagt   tgatcgtaat   ccaacgacct   12060   atgatattga   acttgttcgc   tctggttatc   taaatgccgc   ggcattgaat   accaaaggta   12120   ttgaatttca   agctgcatac   tcattagatc   tagagtcttt   caacgcgcct   ggtgaactac   12180   gcttcaacct   attggggaac   caattacttg   aactagaacg   tcttgaattc   caaaatcgtc   12240   ctgatgagat   taatgatgaa   aaaggcgaag   taggtgatcc   agagctgcag   ttccgcctag   12300   gcatcgatta   ccgtctagat   gatctaagtg   ttagctggaa   cacgcgttat   attgatagcg   12360   tagtaactta   tgatgtctct   gaaaatggtg   gctctcctga   agatttatat   ccaggccaca   12420   taggctcaat   gacaactcat   gacttgagcg   ctacatacta   catcaatgag   aacttcatga   12480   ttaacggtgg   tgtacgtaac   ctatttgacg   cacttccacc   tggatacact   aacgatgcgc   12540   tatatgatct   agttggtcgc   cgtgcattcc   taggtattaa   ggtaatgatg   taattaatta   12600   ttacgcctct   aactaataaa   aatgcaatct   cttcgtagag   attgcatttt   tttatgaaat   12660   ccaatcttaa   actggttctc   cgagcatctt   acgccttaaa   aaccccgccc   ctcaatgtaa   12720   cgccaaagtt   aattgcttac   acgcacttac   acaaacgaac   aatttcatta   acacgagaca   12780   cagctcacgc   tttttatttt   acccttgatt   ttactacata   aaattgcgtt   ttagcgcaca   12840   agtgttctcc   caagctggtc   gtatctgtaa   ttattcagtc   ccaggtgatt   gtattgaccc   12900   ataagctcag   gtagtctgct   ctgccattag   ctaaacaata   ttgacaaaat   ggcgataaaa   12960   tgtggcttag   cgctaagttc   accgtaagtt   ttatcggcat   taagtcccaa   cagattatta   13020   acggaaaccc   gctaaactga   tggcaaaaat   aaatagtgaa   cacttggatg   aagctactat   13080   tacttcgaat   aagtgtacgc   aaacagagac   tgaggctcgg   catagaaatg   ccactacaac   13140   acctgagatg   cgccgattca   tacaagagtc   ggatctcagt   gttagccaac   tgtctaaaat   13200   attaaatatc   agtgaagcta   ccgtacgtaa   gtggcgcaag   cgtgactctg   tcgaaaactg   13260   tcctaatacc   ccgcaccatc   tcaataccac   gctaacccct   ttgcaagaat   atgtggttgt   13320   gggcctgcgt   tatcaattga   aaatgccatt   agacagattg   ctcaaagcaa   cccaagagtt   13380   tatcaatcca   aacgtgtcgc   gctcaggttt   agcaagatgt   ttgaagcgtt   atggcgtttc   13440   acgggtgagt   gatatccaaa   gcccacacgt   accaatgcgc   tactttaatc   aaattccagt   13500   cactcaaggc   agcgatgtgc   aaacctacac   cctgcactat   gaaacgctgg   caaaaacctt   13560   agccttacct   agtaccgatg   gtgacaatgt   ggtgcaagtg   gtgtctctca   ccattccacc   13620   aaagttaacc   gaagaagcac   ccagttcaat   tttgctcggc   attgatcctc   atagcgactg   13680   gatctatctc   gacatatacc   aagatggcaa   tacacaagcc   acgaatagat   atatggctta   13740   tgtgctaaaa   cacgggccat   tccatttacg   aaagttactc   gtgcgtaact   atcacacctt   13800   tttacagcgc   tttcctggag   cgacgcaaaa   tcgccgcccc   tctaaagata   tgcctgaaac   13860   aatcaacaag   acgcctgaaa   cacaggcacc   cagtggagac   tcataatgag   ccagacctct   13920   aaacctacaa   actcagcaac   tgagcaagca   caagactcac   aagctgactc   tcgtttaaat   13980   aaacgactaa   aagatatgcc   aattgctatt   gttggcatgg   cgagtatttt   tgcaaactct   14040   cgctatttga   ataagttttg   ggacttaatc   agcgaaaaaa   ttgatgcgat   tactgaatta   14100   ccatcaactc   actggcagcc   tgaagaatat   tacgacgcag   ataaaaccgc   agcagacaaa   14160   agctactgta   aacgtggtgg   ctttttgcca   gatgtagact   tcaacccaat   ggagtttggc   14220   ctgccgccaa   acattttgga   actgaccgat   tcatcgcaac   tattatcact   catcgttgct   14280   aaagaagtgt   tggctgatgc   taacttacct   gagaattacg   accgcgataa   aattggtatc   14340   accttaggtg   tcggcggtgg   tcaaaaaatt   agccacagcc   taacagcgcg   tctgcaatac   14400   ccagtattga   agaaagtatt   cgccaatagc   ggcattagtg   acaccgacag   cgaaatgctt   14460   atcaagaaat   tccaagacca   atatgtacac   tgggaagaaa   actcgttccc   aggttcactt   14520   ggtaacgtta   ttgcgggccg   tatcgccaac   cgcttcgatt   ttggcggcat   gaactgtgtg   14580   gttgatgctg   cctgtgctgg   atcacttgct   gctatgcgta   tggcgctaac   agagctaact   14640   gaaggtcgct   ctgaaatgat   gatcaccggt   ggtgtgtgta   ctgataactc   accctctatg   14700   tatatgagct   tttcaaaaac   gcccgccttt   accactaacg   aaaccattca   gccatttgat   14760   atcgactcaa   aaggcatgat   gattggtgaa   ggtattggca   tggtggcgct   aaagcgtctt   14820   gaagatgcag   agcgcgatgg   cgaccgcatt   tactctgtaa   ttaaaggtgt   gggtgcatca   14880   tctgacggta   agtttaaatc   aatctatgcc   cctcgcccat   caggccaagc   taaagcactt   14940   aaccgtgcct   atgatgacgc   aggttttgcg   ccgcatacct   taggtctaat   tgaagctcac   15000   ggaacaggta   ctgcagcagg   tgacgcggca   gagtttgccg   gcctttgctc   agtatttgct   15060   gaaggcaacg   ataccaagca   acacattgcg   ctaggttcag   ttaaatcaca   aattggtcat   15120   actaaatcaa   ctgcaggtac   agcaggttta   attaaagctg   ctcttgcttt   gcatcacaag   15180   gtactgccgc   cgaccattaa   cgttagtcag   ccaagcccta   aacttgatat   cgaaaactca   15240   ccgttttatc   taaacactga   gactcgtcca   tggttaccac   gtgttgatgg   tacgccgcgc   15300   cgcgcgggta   ttagctcatt   tggttttggt   ggcactaact   tccattttgt   actagaagag   15360   tacaaccaag   aacacagccg   tactgatagc   gaaaaagcta   agtatcgtca   acgccaagtg   15420   gcgcaaagct   tccttgttag   cgcaagcgat   aaagcatcgc   taattaacga   gttaaacgta   15480   ctagcagcat   ctgcaagcca   agctgagttt   atcctcaaag   atgcagcagc   aaactatggc   15540   gtacgtgagc   ttgataaaaa   tgcaccacgg   atcggtttag   ttgcaaacac   agctgaagag   15600   ttagcaggcc   taattaagca   agcacttgcc   aaactagcag   ctagcgatga   taacgcatgg   15660   cagctacctg   gtggcactag   ctaccgcgcc   gctgcagtag   aaggtaaagt   tgccgcactg   15720   tttgctggcc   aaggttcaca   atatctcaat   atgggccgtg   accttacttg   ttattaccca   15780   gagatgcgtc   agcaatttgt   aactgcagat   aaagtatttg   ccgcaaatga   taaaacgccg   15840   ttatcgcaaa   ctctgtatcc   aaagcctgta   tttaataaag   atgaattaaa   ggctcaagaa   15900   gccattttga   ccaataccgc   caatgcccaa   agcgcaattg   gtgcgatttc   aatgggtcaa   15960   tacgatttgt   ttactgcggc   tggctttaat   gccgacatgg   ttgcaggcca   tagctttggt   16020   gagctaagtg   cactgtgtgc   tgcaggtgtt   atttcagctg   atgactacta   caagctggct   16080   tttgctcgtg   gtgaggctat   ggcaacaaaa   gcaccggcta   aagacggcgt   tgaagcagat   16140   gcaggagcaa   tgtttgcaat   cataaccaag   agtgctgcag   accttgaaac   cgttgaagcc   16200   accatcgcta   aatttgatgg   ggtgaaagtc   gctaactata   acgcgccaac   gcaatcagta   16260   attgcaggcc   caacagcaac   taccgctgat   gcggctaaag   cgctaactga   gcttggttac   16320   aaagcgatta   acctgccagt   atcaggtgca   ttccacactg   aacttgttgg   tcacgctcaa   16380   gcgccatttg   ctaaagcgat   tgacgcagcc   aaatttacta   aaacaagccg   agcactttac   16440   tcaaatgcaa   ctggcggact   ttatgaaagc   actgctgcaa   agattaaagc   ctcgtttaag   16500   aaacatatgc   ttcaatcagt   gcgctttact   agccagctag   aagccatgta   caacgacggc   16560   gcccgtgtat   ttgttgaatt   tggtccaaag   aacatcttac   aaaaattagt   tcaaggcacg   16620   cttgtcaaca   ctgaaaatga   agtttgcact   atctctatca   accctaatcc   taaagttgat   16680   agtgatctgc   agcttaagca   agcagcaatg   cagctagcgg   ttactggtgt   ggtactcagt   16740   gaaattgacc   cataccaagc   cgatattgcc   gcaccagcga   aaaagtcgcc   aatgagcatt   16800   tcgcttaatg   ctgctaacca   tatcagcaaa   gcaactcgcg   ctaagatggc   caagtcttta   16860   gagacaggta   tcgtcacctc   gcaaatagaa   catgttattg   aagaaaaaat   cgttgaagtt   16920   gagaaactgg   ttgaagtcga   aaagatcgtc   gaaaaagtgg   ttgaagtaga   gaaagttgtt   16980   gaggttgaag   ctcctgttaa   ttcagtgcaa   gccaatgcaa   ttcaaacccg   ttcagttgtc   17040   gctccagtaa   tagagaacca   agtcgtgtct   aaaaacagta   agccagcagt   ccagagcatt   17100   agtggtgatg   cactcagcaa   cttttttgct   gcacagcagc   aaaccgcaca   gttgcatcag   17160   cagttcttag   ctattccgca   gcaatatggt   gagacgttca   ctacgctgat   gaccgagcaa   17220   gctaaactgg   caagttctgg   tgttgcaatt   ccagagagtc   tgcaacgctc   aatggagcaa   17280   ttccaccaac   tacaagcgca   aacactacaa   agccacaccc   agttccttga   gatgcaagcg   17340   ggtagcaaca   ttgcagcgtt   aaacctactc   aatagcagcc   aagcaactta   cgctccagcc   17400   attcacaatg   aagcgattca   aagccaagtg   gttcaaagcc   aaactgcagt   ccagccagta   17460   atttcaacac   aagttaacca   tgtgtcagag   cagccaactc   aagctccagc   tccaaaagcg   17520   cagccagcac   ctgtgacaac   tccagttcaa   actgctccgg   cacaagttgt   tcgtcaagcc   17580   gcaccagttc   aagccgctat   tgaaccgatt   aatacaagtg   ttgcgactac   aacgccttca   17640   gccttcagcg   ccgaaacagc   cctgagcgca   acaaaagtcc   aagccactat   gcttgaagtg   17700   gttgctgaga   aaaccggtta   cccaactgaa   atgctagagc   ttgaaatgga   tatggaagcc   17760   gatttaggca   tcgattctat   caagcgtgta   gaaattcttg   gcacagtaca   agatgagcta   17820   ccgggtctac   ctgagcttag   ccctgaagat   ctagctgagt   gtcgaacgct   aggcgaaatc   17880   gttgactata   tgggcagtaa   actgccggct   gaaggctcta   tgaattctca   gctgtctaca   17940   ggttccgcag   ctgcgactcc   tgcagcgaat   ggtctttctg   cggagaaagt   tcaagcgact   18000   atgatgtctg   tggttgccga   aaagactggc   tacccaactg   aaatgctaga   gcttgaaatg   18060   gatatggaag   ccgatttagg   catagattct   atcaagcgcg   ttgaaattct   tggcacagta   18120   caagatgagc   taccgggtct   acctgagctt   agccctgaag   atctagctga   gtgtcgtact   18180   ctaggcgaaa   tcgttgacta   tatgaactct   aaactcgctg   acggctctaa   gctgccggct   18240   gaaggctcta   tgaattctca   gctgtctaca   agtgccgcag   ctgcgactcc   tgcagcgaat   18300   ggtctctctg   cggagaaagt   tcaagcgact   atgatgtctg   tggttgccga   aaagactggc   18360   tacccaactg   aaatgctaga   acttgaaatg   gatatggaag   ctgaccttgg   catcgattca   18420   atcaagcgcg   ttgaaattct   tggcacagta   caagatgagc   taccgggttt   acctgagcta   18480   aatccagaag   atttggcaga   gtgtcgtact   cttggcgaaa   tcgtgactta   tatgaactct   18540   aaactcgctg   acggctctaa   gctgccagct   gaaggctcta   tgcactatca   gctgtctaca   18600   agtaccgctg   ctgcgactcc   tgtagcgaat   ggtctctctg   cagaaaaagt   tcaagcgacc   18660   atgatgtctg   tagttgcaga   taaaactggc   tacccaactg   aaatgcttga   acttgaaatg   18720   gatatggaag   ccgatttagg   tatcgattct   atcaagcgcg   ttgaaattct   tggcacagta   18780   caagatgagc   taccgggttt   acctgagcta   aatccagaag   atctagcaga   gtgtcgcacc   18840   ctaggcgaaa   tcgttgacta   tatgggcagt   aaactgccgg   ctgaaggctc   tgctaataca   18900   agtgccgctg   cgtctcttaa   tgttagtgcc   gttgcggcgc   ctcaagctgc   tgcgactcct   18960   gtatcgaacg   gtctctctgc   agagaaagtg   caaagcacta   tgatgtcagt   agttgcagaa   19020   aagaccggct   acccaactga   aatgctagaa   cttggcatgg   atatggaagc   cgatttaggt   19080   atcgactcaa   ttaaacgcgt   tgagattctt   ggcacagtac   aagatgagct   accgggtcta   19140   ccagagctta   atcctgaaga   tttagctgag   tgccgtacgc   tgggcgaaat   cgttgactat   19200   atgaactcta   agctggctga   cggctctaag   cttccagctg   aaggctctgc   taatacaagt   19260   gccactgctg   cgactcctgc   agtgaatggt   ctttctgctg   acaaggtaca   ggcgactatg   19320   atgtctgtag   ttgctgaaaa   gaccggctac   ccaactgaaa   tgctagaact   tggcatggat   19380   atggaagcag   accttggtat   tgattctatt   aagcgcgttg   aaattcttgg   cacagtacaa   19440   gatgagctcc   caggtttacc   tgagcttaat   cctgaagatc   tcgctgagtg   ccgcacgctt   19500   ggcgaaatcg   ttagctatat   gaactctcaa   ctggctgatg   gctctaaact   ttctacaagt   19560   gcggctgaag   gctctgctga   tacaagtgct   gcaaatgctg   caaagccggc   agcaatttcg   19620   gcagaaccaa   gtgttgagct   tcctcctcat   agcgaggtag   cgctaaaaaa   gcttaatgcg   19680   gcgaacaagc   tagaaaattg   tttcgccgca   gacgcaagtg   ttgtgattaa   cgatgatggt   19740   cacaacgcag   gcgttttagc   tgagaaactt   attaaacaag   gcctaaaagt   agccgttgtg   19800   cgtttaccga   aaggtcagcc   tcaatcgcca   ctttcaagcg   atgttgctag   ctttgagctt   19860   gcctcaagcc   aagaatctga   gcttgaagcc   agtatcactg   cagttatcgc   gcagattgaa   19920   actcaggttg   gcgctattgg   tggctttatt   cacttgcaac   cagaagcgaa   tacagaagag   19980   caaacggcag   taaacctaga   tgcgcaaagt   tttactcacg   ttagcaatgc   gttcttgtgg   20040   gccaaattat   tgcaaccaaa   gctcgttgct   ggagcagatg   cgcgtcgctg   ttttgtaaca   20100   gtaagccgta   tcgacggtgg   ctttggttac   ctaaatactg   acgccctaaa   agatgctgag   20160   ctaaaccaag   cagcattagc   tggtttaact   aaaaccttaa   gccatgaatg   gccacaagtg   20220   ttctgtcgcg   cgctagatat   tgcaacagat   gttgatgcaa   cccatcttgc   tgatgcaatc   20280   accagtgaac   tatttgatag   ccaagctcag   ctacctgaag   tgggcttaag   cttaattgat   20340   ggcaaagtta   accgcgtaac   tctagttgct   gctgaagctg   cagataaaac   agcaaaagca   20400   gagcttaaca   gcacagataa   aatcttagtg   actggtgggg   caaaaggggt   gacatttgaa   20460   tgtgcactgg   cattagcatc   tcgcagccag   tctcacttta   tcttagctgg   gcgcagtgaa   20520   ttacaagctt   taccaagctg   ggctgagggt   aagcaaacta   gcgagctaaa   atcagctgca   20580   atcgcacata   ttatttctac   tggtcaaaag   ccaacgccta   agcaagttga   agccgctgtg   20640   tggccagtgc   aaagcagcat   tgaaattaat   gccgccctag   ccgcctttaa   caaagttggc   20700   gcctcagctg   aatacgtcag   catggatgtt   accgatagcg   ccgcaatcac   agcagcactt   20760   aatggtcgct   caaatgagat   caccggtctt   attcatggcg   caggtgtact   agccgacaag   20820   catattcaag   acaagactct   tgctgaactt   gctaaagttt   atggcactaa   agtcaacggc   20880   ctaaaagcgc   tgctcgcggc   acttgagcca   agcaaaatta   aattacttgc   tatgttctca   20940   tctgcagcag   gtttttacgg   taatatcggc   caaagcgatt   acgcgatgtc   gaacgatatt   21000   cttaacaagg   cagcgctgca   gttcaccgct   cgcaacccac   aagctaaagt   catgagcttt   21060   aactggggtc   cttgggatgg   cggcatggtt   aacccagcgc   ttaaaaagat   gtttaccgag   21120   cgtggtgtgt   acgttattcc   actaaaagca   ggtgcagagc   tatttgccac   tcagctattg   21180   gctgaaactg   gcgtgcagtt   gctcattggt   acgtcaatgc   aaggtggcag   cgacactaaa   21240   gcaactgaga   ctgcttctgt   aaaaaagctt   aatgcgggtg   aggtgctaag   tgcatcgcat   21300   ccgcgtgctg   gtgcacaaaa   aacaccacta   caagctgtca   ctgcaacgcg   tctgttaacc   21360   ccaagtgcca   tggtcttcat   tgaagatcac   cgcattggcg   gtaacagtgt   gttgccaacg   21420   gtatgcgcca   tcgactggat   gcgtgaagcg   gcaagcgaca   tgcttggcgc   tcaagttaag   21480   gtacttgatt   acaagctatt   aaaaggcatt   gtatttgaga   ctgatgagcc   gcaagagtta   21540   acacttgagc   taacgccaga   cgattcagac   gaagctacgc   tacaagcatt   aatcagctgt   21600   aatgggcgtc   cgcaatacaa   ggcgacgctt   atcagtgata   atgccgatat   taagcaactt   21660   aacaagcagt   ttgatttaag   cgctaaggcg   attaccacag   caaaagagct   ttatagcaac   21720   ggcaccttgt   tccacggtcc   gcgtctacaa   gggatccaat   ctgtagtgca   gttcgatgat   21780   caaggcttaa   ttgctaaagt   cgctctgcct   aaggttgaac   ttagcgattg   tggtgagttc   21840   ttgccgcaaa   cccacatggg   tggcagtcaa   ccttttgctg   aggacttgct   attacaagct   21900   atgctggttt   gggctcgcct   taaaactggc   tcggcaagtt   tgccatcaag   cattggtgag   21960   tttacctcat   accaaccaat   ggcctttggt   gaaactggta   ccatagagct   tgaagtgatt   22020   aagcacaaca   aacgctcact   tgaagcgaat   gttgcgctat   atcgtgacaa   cggcgagtta   22080   agtgccatgt   ttaagtcagc   taaaatcacc   attagcaaaa   gcttaaattc   agcattttta   22140   cctgctgtct   tagcaaacga   cagtgaggcg   aattagtgga   acaaacgcct   aaagctagtg   22200   cgatgccgct   gcgcatcgca   cttatcttac   tgccaacacc   gcagtttgaa   gttaactctg   22260   tcgaccagtc   agtattagcc   agctatcaaa   cactgcagcc   tgagctaaat   gccctgctta   22320   atagtgcgcc   gacacctgaa   atgctcagca   tcactatctc   agatgatagc   gatgcaaaca   22380   gctttgagtc   gcagctaaat   gctgcgacca   acgcaattaa   caatggctat   atcgtcaagc   22440   ttgctacggc   aactcacgct   ttgttaatgc   tgcctgcatt   aaaagcggcg   caaatgcgga   22500   tccatcctca   tgcgcagctt   gccgctatgc   agcaagctaa   atcgacgcca   atgagtcaag   22560   tatctggtga   gctaaagctt   ggcgctaatg   cgctaagcct   agctcagact   aatgcgctgt   22620   ctcatgcttt   aagccaagcc   aagcgtaact   taactgatgt   cagcgtgaat   gagtgttttg   22680   agaacctcaa   aagtgaacag   cagttcacag   aggtttattc   gcttattcag   caacttgcta   22740   gccgcaccca   tgtgagaaaa   gaggttaatc   aaggtgtgga   acttggccct   aaacaagcca   22800   aaagccacta   ttggtttagc   gaatttcacc   aaaaccgtgt   tgctgccatc   aactttatta   22860   atggccaaca   agcaaccagc   tatgtgctta   ctcaaggttc   aggattgtta   gctgcgaaat   22920   caatgctaaa   ccagcaaaga   ttaatgttta   tcttgccggg   taacagtcag   caacaaataa   22980   ccgcatcaat   aactcagtta   atgcagcaat   tagagcgttt   gcaggtaact   gaggttaatg   23040   agctttctct   agaatgccaa   ctagagctgc   tcagcataat   gtatgacaac   ttagtcaacg   23100   cagacaaact   cactactcgc   gatagtaagc   ccgcttatca   ggctgtgatt   caagcaagct   23160   ctgttagcgc   tgcaaagcaa   gagttaagcg   cgcttaacga   tgcactcaca   gcgctgtttg   23220   ctgagcaaac   aaacgccaca   tcaacgaata   aaggcttaat   ccaatacaaa   acaccggcgg   23280   gcagttactt   aaccctaaca   ccgcttggca   gcaacaatga   caacgcccaa   gcgggtcttg   23340   cttttgtcta   tccgggtgtg   ggaacggttt   acgccgatat   gcttaatgag   ctgcatcagt   23400   acttccctgc   gctttacgcc   aaacttgagc   gtgaaggcga   tttaaaggcg   atgctacaag   23460   cagaagatat   ctatcatctt   gaccctaaac   atgctgccca   aatgagctta   ggtgacttag   23520   ccattgctgg   cgtggggagc   agctacctgt   taactcagct   gctcaccgat   gagtttaata   23580   ttaagcctaa   ttttgcatta   ggttactcaa   tgggtgaagc   atcaatgtgg   gcaagcttag   23640   gcgtatggca   aaacccgcat   gcgctgatca   gcaaaaccca   aaccgacccg   ctatttactt   23700   ctgctatttc   cggcaaattg   accgcggtta   gacaagcttg   gcagcttgat   gataccgcag   23760   cggaaatcca   gtggaatagc   tttgtggtta   gaagtgaagc   agcgccgatt   gaagccttgc   23820   taaaagatta   cccacacgct   tacctcgcga   ttattcaagg   ggatacctgc   gtaatcgctg   23880   gctgtgaaat   ccaatgtaaa   gcgctacttg   cagcactggg   taaacgcggt   attgcagcta   23940   atcgtgtaac   ggcgatgcat   acgcagcctg   cgatgcaaga   gcatcaaaat   gtgatggatt   24000   tttatctgca   accgttaaaa   gcagagcttc   ctagtgaaat   aagctttatc   agcgccgctg   24060   atttaactgc   caagcaaacg   gtgagtgagc   aagcacttag   cagccaagtc   gttgctcagt   24120   ctattgccga   caccttctgc   caaaccttgg   actttaccgc   gctagtacat   cacgcccaac   24180   atcaaggcgc   taagctgttt   gttgaaattg   gcgcggatag   acaaaactgc   accttgatag   24240   acaagattgt   taaacaagat   ggtgccagca   gtgtacaaca   tcaaccttgt   tgcacagtgc   24300   ctatgaacgc   aaaaggtagc   caagatatta   ccagcgtgat   taaagcgctt   ggccaattaa   24360   ttagccatca   ggtgccatta   tcggtgcaac   catttattga   tggactcaag   cgcgagctaa   24420   cactttgcca   attgaccagc   caacagctgg   cagcacatgc   aaatgttgac   agcaagtttg   24480   agtctaacca   agaccattta   cttcaagggg   aagtctaatg   tcattaccag   acaatgcttc   24540   taaccacctt   tctgccaacc   agaaaggcgc   atctcaggca   agtaaaacca   gtaagcaaag   24600   caaaatcgcc   attgtcggtt   tagccactct   gtatccagac   gctaaaaccc   cgcaagaatt   24660   ttggcagaat   ttgctggata   aacgcgactc   tcgcagcacc   ttaactaacg   aaaaactcgg   24720   cgctaacagc   caagattatc   aaggtgtgca   aggccaatct   gaccgttttt   attgtaataa   24780   aggcggctac   attgagaact   tcagctttaa   tgctgcaggc   tacaaattgc   cggagcaaag   24840   cttaaatggc   ttggacgaca   gcttcctttg   ggcgctcgat   actagccgta   acgcactaat   24900   tgatgctggt   attgatatca   acggcgctga   tttaagccgc   gcaggtgtag   tcatgggcgc   24960   gctgtcgttc   ccaactaccc   gctcaaacga   tctgtttttg   ccaatttatc   acagcgccgt   25020   tgaaaaagcc   ctgcaagata   aactaggcgt   aaaggcattt   aagctaagcc   caactaatgc   25080   tcataccgct   cgcgcggcaa   atgagagcag   cctaaatgca   gccaatggtg   ccattgccca   25140   taacagctca   aaagtggtgg   ccgatgcact   tggccttggc   ggcgcacaac   taagcctaga   25200   tgctgcctgt   gctagttcgg   tttactcatt   aaagcttgcc   tgcgattacc   taagcactgg   25260   caaagccgat   atcatgctag   caggcgcagt   atctggcgcg   gatcctttct   ttattaatat   25320   gggattctca   atcttccacg   cctacccaga   ccatggtatc   tcagtaccgt   ttgatgccag   25380   cagtaaaggt   ttgtttgctg   gcgaaggcgc   tggcgtatta   gtgcttaaac   gtcttgaaga   25440   tgccgagcgc   gacaatgaca   aaatctatgc   ggttgttagc   ggcgtaggtc   tatcaaacga   25500   cggtaaaggc   cagtttgtat   taagccctaa   tccaaaaggt   caggtgaagg   cctttgaacg   25560   tgcttatgct   gccagtgaca   ttgagccaaa   agacattgaa   gtgattgagt   gccacgcaac   25620   aggcacaccg   cttggcgata   aaattgagct   cacttcaatg   gaaaccttct   ttgaagacaa   25680   gctgcaaggc   accgatgcac   cgttaattgg   ctcagctaag   tctaacttag   gccacctatt   25740   aactgcagcg   ggcatgccgg   ggatcatgaa   gatgatcttc   gccatgaaag   aaggttacct   25800   gccgccaagt   atcaatatta   gtgatgctat   cgcttcgccg   aaaaaactct   tcggtaaacc   25860   aaccctgcct   agcatggttc   aaggctggcc   agataagcca   tcgaataatc   attttggtgt   25920   aagaacccgt   cacgcaggcg   tatcggtatt   tggctttggt   ggctgtaacg   cccatctgtt   25980   gcttgagtca   tacaacggca   aaggaacagt   aaaggcagaa   gccactcaag   taccgcgtca   26040   agctgagccg   ctaaaagtgg   ttggccttgc   ctcgcacttt   gggcctctta   gcagcattaa   26100   tgcactcaac   aatgctgtga   cccaagatgg   gaatggcttt   atcgaactgc   cgaaaaagcg   26160   ctggaaaggc   cttgaaaagc   acagtgaact   gttagctgaa   tttggcttag   catctgcgcc   26220   aaaaggtgct   tatgttgata   acttcgagct   ggacttttta   cgctttaaac   tgccgccaaa   26280   cgaagatgac   cgtttgatct   cacagcagct   aatgctaatg   cgagtaacag   acgaagccat   26340   tcgtgatgcc   aagcttgagc   cggggcaaaa   agtagctgta   ttagtggcaa   tggaaactga   26400   gcttgaactg   catcagttcc   gcggccgggt   taacttgcat   actcaattag   cgcaaagtct   26460   tgccgccatg   ggcgtgagtt   tatcaacgga   tgaataccaa   gcgcttgaag   ccatcgccat   26520   ggacagcgtg   cttgatgctg   ccaagctcaa   tcagtacacc   agctttattg   gtaatattat   26580   ggcgtcacgc   gtggcgtcac   tatgggactt   taatggccca   gccttcacta   tttcagcagc   26640   agagcaatct   gtgagccgct   gtatcgatgt   ggcgcaaaac   ctcatcatgg   aggataacct   26700   agatgcggtg   gtgattgcag   cggtcgatct   ctctggtagc   tttgagcaag   tcattcttaa   26760   aaatgccatt   gcacctgtag   ccattgagcc   aaacctcgaa   gcaagcctta   atccaacatc   26820   agcaagctgg   aatgtcggtg   aaggtgctgg   cgcggtcgtg   cttgttaaaa   atgaagctac   26880   atcgggctgc   tcatacggcc   aaattgatgc   acttggcttt   gctaaaactg   ccgaaacagc   26940   gttggctacc   gacaagctac   tgagccaaac   tgccacagac   tttaataagg   ttaaagtgat   27000   tgaaactatg   gcagcgcctg   ctagccaaat   tcaattagcg   ccaatagtta   gctctcaagt   27060   gactcacact   gctgcagagc   agcgtgttgg   tcactgcttt   gctgcagcgg   gtatggcaag   27120   cctattacac   ggcttactta   acttaaatac   tgtagcccaa   accaataaag   ccaattgcgc   27180   gcttatcaac   aatatcagtg   aaaaccaatt   atcacagctg   ttgattagcc   aaacagcgag   27240   cgaacaacaa   gcattaaccg   cgcgtttaag   caatgagctt   aaatccgatg   ctaaacacca   27300   actggttaag   caagtcacct   taggtggccg   tgatatctac   cagcatattg   ttgatacacc   27360   gcttgcaagc   cttgaaagca   ttactcagaa   attggcgcaa   gcgacagcat   cgacagtggt   27420   caaccaagtt   aaacctatta   aggccgctgg   ctcagtcgaa   atggctaact   cattcgaaac   27480   ggaaagctca   gcagagccac   aaataacaat   tgcagcacaa   cagactgcaa   acattggcgt   27540   caccgctcag   gcaaccaaac   gtgaattagg   taccccacca   atgacaacaa   ataccattgc   27600   taatacagca   aataatttag   acaagactct   tgagactgtt   gctggcaata   ctgttgctag   27660   caaggttggc   tctggcgaca   tagtcaattt   tcaacagaac   caacaattgg   ctcaacaagc   27720   tcacctcgcc   tttcttgaaa   gccgcagtgc   gggtatgaag   gtggctgatg   ctttattgaa   27780   gcaacagcta   gctcaagtaa   caggccaaac   tatcgataat   caggccctcg   atactcaagc   27840   cgtcgatact   caaacaagcg   agaatgtagc   gattgccgca   gaatcaccag   ttcaagttac   27900   aacacctgtt   caagttacaa   cacctgttca   aatcagtgtt   gtggagttaa   aaccagatca   27960   cgctaatgtg   ccaccataca   cgccgccagt   gcctgcatta   aagccgtgta   tctggaacta   28020   tgccgattta   gttgagtacg   cagaaggcga   tatcgccaag   gtatttggca   gtgattatgc   28080   cattatcgac   agctactcgc   gccgcgtacg   tctaccgacc   actgactacc   tgttggtatc   28140   gcgcgtgacc   aaacttgatg   cgaccatcaa   tcaatttaag   ccatgctcaa   tgaccactga   28200   gtacgacatc   cctgttgatg   cgccgtactt   agtagacgga   caaatccctt   gggcggtagc   28260   agtagaatca   ggccaatgtg   acttgatgct   tattagctat   ctcggtatcg   actttgagaa   28320   caaaggcgag   cgggtttatc   gactactcga   ttgtaccctc   accttcctag   gcgacttgcc   28380   acgtggcgga   gataccctac   gttacgacat   taagatcaat   aactatgctc   gcaacggcga   28440   caccctgctg   ttcttcttct   cgtatgagtg   ttttgttggc   gacaagatga   tcctcaagat   28500   ggatggcggc   tgcgctggct   tcttcactga   tgaagagctt   gccgacggta   aaggcgtgat   28560   tcgcacagaa   gaagagatta   aagctcgcag   cctagtgcaa   aagcaacgct   ttaatccgtt   28620   actagattgt   cctaaaaccc   aatttagtta   tggtgatatt   cataagctat   taactgctga   28680   tattgagggt   tgttttggcc   caagccacag   tggcgtccac   cagccgtcac   tttgtttcgc   28740   atctgaaaaa   ttcttgatga   ttgaacaagt   cagcaaggtt   gatcgcactg   gcggtacttg   28800   gggacttggc   ttaattgagg   gtcataagca   gcttgaagca   gaccactggt   acttcccatg   28860   tcatttcaag   ggcgaccaag   tgatggctgg   ctcgctaatg   gctgaaggtt   gtggccagtt   28920   attgcagttc   tatatgctgc   accttggtat   gcatacccaa   actaaaaatg   gtcgtttcca   28980   acctcttgaa   aacgcctcac   agcaagtacg   ctgtcgcggt   caagtgctgc   cacaatcagg   29040   cgtgctaact   taccgtatgg   aagtgactga   aatcggtttc   agtccacgcc   catatgctaa   29100   agctaacatc   gatatcttgc   ttaatggcaa   agcggtagtg   gatttccaaa   acctaggggt   29160   gatgataaaa   gaggaagatg   agtgtactcg   ttatccactt   ttgactgaat   caacaacggc   29220   tagcactgca   caagtaaacg   ctcaaacaag   tgcgaaaaag   gtatacaagc   cagcatcagt   29280   caatgcgcca   ttaatggcac   aaattcctga   tctgactaaa   gagccaaaca   agggcgttat   29340   tccgatttcc   catgttgaag   caccaattac   gccagactac   ccgaaccgtg   tacctgatac   29400   agtgccattc   acgccgtatc   acatgtttga   gtttgctaca   ggcaatatcg   aaaactgttt   29460   cgggccagag   ttctcaatct   atcgcggcat   gatcccacca   cgtacaccat   gcggtgactt   29520   acaagtgacc   acacgtgtga   ttgaagttaa   cggtaagcgt   ggcgacttta   aaaagccatc   29580   atcgtgtatc   gctgaatatg   aagtgcctgc   agatgcgtgg   tatttcgata   aaaacagcca   29640   cggcgcagtg   atgccatatt   caattttaat   ggagatctca   ctgcaaccta   acggctttat   29700   ctcaggttac   atgggcacaa   ccctaggctt   ccctggcctt   gagctgttct   tccgtaactt   29760   agacggtagc   ggtgagttac   tacgtgaagt   agatttacgt   ggtaaaacca   tccgtaacga   29820   ctcacgttta   ttatcaacag   tgatggccgg   cactaacatc   atccaaagct   ttagcttcga   29880   gctaagcact   gacggtgagc   ctttctatcg   cggcactgcg   gtatttggct   attttaaagg   29940   tgacgcactt   aaagatcagc   taggcctaga   taacggtaaa   gtcactcagc   catggcatgt   30000   agctaacggc   gttgctgcaa   gcactaaggt   gaacctgctt   gataagagct   gccgtcactt   30060   taatgcgcca   gctaaccagc   cacactatcg   tctagccggt   ggtcagctga   actttatcga   30120   cagtgttgaa   attgttgata   atggcggcac   cgaaggttta   ggttacttgt   atgccgagcg   30180   caccattgac   ccaagtgatt   ggttcttcca   gttccacttc   caccaagatc   cggttatgcc   30240   aggctcctta   ggtgttgaag   caattattga   aaccatgcaa   gcttacgcta   ttagtaaaga   30300   cttgggcgca   gatttcaaaa   atcctaagtt   tggtcagatt   ttatcgaaca   tcaagtggaa   30360   gtatcgcggt   caaatcaatc   cgctgaacaa   gcagatgtct   atggatgtca   gcattacttc   30420   aatcaaagat   gaagacggta   agaaagtcat   cacaggtaat   gccagcttga   gtaaagatgg   30480   tctgcgcata   tacgaggtct   tcgatatagc   tatcagcatc   gaagaatctg   tataaatcgg   30540   agtgactgtc   tggctatttt   actcaatttc   tgtgtcaaaa   gtgctcacct   atattcatag   30600   gctgcgcgct   tttttctgga   aattgagcaa   aagtatctgc   gtcctaactc   gatttataag   30660   aatggtttaa   ttgaaaagaa   caacagctaa   gagccgcaag   ctcaatataa   ataattaagg   30720   gtcttacaaa   taatgaatcc   tacagcaact   aacgaaatgc   tttctccgtg   gccatgggct   30780   gtgacagagt   caaatatcag   ttttgacgtg   caagtgatgg   aacaacaact   taaagatttt   30840   agccgggcat   gttacgtggt   caatcatgcc   gaccacggct   ttggtattgc   gcaaactgcc   30900   gatatcgtga   ctgaacaagc   ggcaaacagc   acagatttac   ctgttagtgc   ttttactcct   30960   gcattaggta   ccgaaagcct   aggcgacaat   aatttccgcc   gcgttcacgg   cgttaaatac   31020   gcttattacg   caggcgctat   ggcaaacggt   atttcatctg   aagagctagt   gattgcccta   31080   ggtcaagctg   gcattttgtg   ttcgtttgga   gcagccggtc   ttattccaag   tcgcgttgaa   31140   gcggcaatta   accgtattca   agcagcgctg   ccaaatggcc   cttatatgtt   taaccttatc   31200   catagtccta   gcgagccagc   attagagcgt   ggcagcgtag   agctattttt   aaagcataag   31260   gtacgcaccg   ttgaagcatc   agctttctta   ggtctaacac   cacaaatcgt   ctattaccgt   31320   gcagcaggat   tgagccgaga   cgcacaaggt   aaagttgtgg   ttggtaacaa   ggttatcgct   31380   aaagtaagtc   gcaccgaagt   ggctgaaaag   tttatgatgc   cagcgcccgc   aaaaatgcta   31440   caaaaactag   ttgatgacgg   ttcaattacc   gctgagcaaa   tggagctggc   gcaacttgta   31500   cctatggctg   acgacatcac   tgcagaggcc   gattcaggtg   gccatactga   taaccgtcca   31560   ttagtaacat   tgctgccaac   cattttagcg   ctgaaagaag   aaattcaagc   taaataccaa   31620   tacgacactc   ctattcgtgt   cggttgtggt   ggcggtgtgg   gtacgcctga   tgcagcgctg   31680   gcaacgttta   acatgggcgc   ggcgtatatt   gttaccggct   ctatcaacca   agcttgtgtt   31740   gaagcgggcg   caagtgatca   cactcgtaaa   ttacttgcca   ccactgaaat   ggccgatgtg   31800   actatggcac   cagctgcaga   tatgttcgag   atgggcgtaa   aactgcaggt   ggttaagcgc   31860   ggcacgctat   tcccaatgcg   cgctaacaag   ctatatgaga   tctacacccg   ttacgattca   31920   atcgaagcga   tcccattaga   cgagcgtgaa   aagcttgaga   aacaagtatt   ccgctcaagc   31980   ctagatgaaa   tatgggcagg   tacagtggcg   cactttaacg   agcgcgaccc   taagcaaatc   32040   gaacgcgcag   agggtaaccc   taagcgtaaa   atggcattga   ttttccgttg   gtacttaggt   32100   ctttctagtc   gctggtcaaa   ctcaggcgaa   gtgggtcgtg   aaatggatta   tcaaatttgg   32160   gctggccctg   ctctcggtgc   atttaaccaa   tgggcaaaag   gcagttactt   agataactat   32220   caagaccgaa   atgccgtcga   tttggcaaag   cacttaatgt   acggcgcggc   ttacttaaat   32280   cgtattaact   cgctaacggc   tcaaggcgtt   aaagtgccag   cacagttact   tcgctggaag   32340   ccaaaccaaa   gaatggccta   atacacttac   aaagcaccag   tctaaaaagc   cactaatctt   32400   gattagtggc   tttttttatt   gtggtcaata   tgaggctatt   tagcctgtaa   gcctgaaaat   32460   atcagcactc   tgactttaca   agcaaattat   aattaaggca   gggctctact   catttatact   32520   gctagcaaac   aagcaagttg   cccagtaaaa   caacaaggta   cctgatttat   atcgtcataa   32580   aagttggcta   gagattcgtt   attgatcttt   actgattaga   gtcgctctgt   ttggaaaaag   32640   gtttctcgtt   atcatcaaaa   tacactctca   aacctttaat   caattacaac   ttaggctttc   32700   tgcgggcatt   tttatcttat   ttgccacagc   tgtatttgcc   tttaggtttt   gggtgcaact   32760   accattaatt   gaggcctcat   tagttaaatt   atctgagcaa   gagctcacct   ctttaaatta   32820   cgcttttcag   caaatgagaa   agccactaca   aaccattaat   tacgactatg   cggtgtggga   32880   cagaacctac   agctatatga   aatcaaactc   agcgagcgct   aaaaggtact   atgaaaaaca   32940   tgagtaccca   gatgatacgt   tcaagagttt   aaaagtcgac   ggagtattta   tattcaaccg   33000   tacaaatcag   ccagttttta   gtaaaggttt   taatcataga   aatgatatac   cgctggtctt   33060   tgaattaact   gactttaaac   aacatccaca   aaacatcgca   ttatctccac   aaaccaaaca   33120   ggcacaccca   ccggcaagta   agccgttaga   ctcccctgat   gatgtgcctt   ctacccatgg   33180   ggttatcgcc   acacgatacg   gtccagcaat   ttatagctct   accagcattt   taaaatctga   33240   tcgtagcggc   tcccaacttg   gttatttagt   cttcattagg   ttaattgatg   aatggttcat   33300   cgctgagcta   tcgcaataca   ctgccgcagg   tgttgaaatc   gctatggctg   atgccgcaga   33360   cgcacaatta   gcgagattag   gcgcaaacac   taagcttaat   aaagtaaccg   ctacatccga   33420   acggttaata   actaatgtcg   atggtaagcc   tctgttgaag   ttagtgcttt   accataccaa   33480   taaccaaccg   ccgccgatgc   tagattacag   tataataatt   ctattagttg   agatgtcatt   33540   tttactgatc   ctcgcttatt   tcctttactc   ctacttctta   gtcaggccag   ttagaaagct   33600   ggcttcagat   attaaaaaaa   tggataaaag   tcgtgaaatt   aaaaagctaa   ggtatcacta   33660   ccctattact   gagctagtca   aagttgcgac   tcacttcaac   gccctaatgg   ggacgattca   33720   ggaacaaact   aaacagctta   atgaacaagt   ttttattgat   aaattaacca   atattcccaa   33780   tcgtcgcgct   tttgagcagc   gacttgaaac   ctattgccaa   ctgctagccc   ggcaacaaat   33840   tggctttact   ctcatcattg   ccgatgtgga   tcattttaaa   gagtacaacg   atactcttgg   33900   gcaccttgct   ggggatgaag   cattaataaa   agtggcacaa   acactatcgc   aacagtttta   33960   ccgtgcagaa   gatatttgtg   cccgttttgg   tggtgaagaa   tttattatgt   tatttcgaga   34020   catacctgat   gagcccttgc   agagaaagct   cgatgcgatg   ctgcactctt   ttgcagagct   34080   caacctacct   catccaaact   catcaaccgc   taattacgtt   actgtgagcc   ttggggtttg   34140   cacagttgtt   gctgttgatg   attttgaatt   taaaagtgag   tcgcatatta   ttggcagtca   34200   ggctgcatta   atcgcagata   aggcgcttta   tcatgctaaa   gcctgtggtc   gtaaccagtt   34260   gtcaaaaact   actattactg   ttgatgagat   tgagcaatta   gaagcaaata   aaatcggtca   34320   tcaagcctaa   actcgttcga   gtactttccc   ctaagtcaga   gctatttgcc   acttcaagat   34380   gtggctacaa   ggcttactct   ttcaaaacct   gcatcaatag   aacacagcaa   aatacaataa   34440   tttaagtcaa   tttagcctat   taaacagagt   taatgacagc   tcatggtcgc   aacttattag   34500   ctatttctag   caatataaaa   acttatccat   tagtagtaac   caataaaaaa   actaatatat   34560   aaaactattt   aatcattatt   ttacagatga   ttagctacca   cccaccttaa   gctggctata   34620   ttcgcactag   taaaaataaa   cattagatcg   ggttcagatc   aatttacgag   tctcgtataa   34680   aatgtacaat   aattcactta   atttaatact   gcatattttt   acaagtagag   agcggtgatg   34740   aaacaaaata   cgaaaggctt   tacattaatt   gaattagtca   tcgtgattat   tattctcggt   34800   atacttgctg   ctgtggcact   gccgaaattc   atcaatgttc   aagatgacgc   taggatctct   34860   gcgatgagcg   gtcagttttc   atcatttgaa   agtgccgtaa   aactatacca   tagcggttgg   34920   ttagccaaag   gctacaacac   tgcggttgaa   aagctctcag   gctttggcca   aggtaatgtt   34980   gcatcaagtg   acacaggttt   tccgtactca   acatcaggca   cgagtactga   tgtgcataaa   35040   gcttgtggtg   aactatggca   tggcattacc   gatacagact   tcacaattgg   tgcggttagt   35100   gatggcgatc   taatgactgc   agatgtcgat   attgcttaca   cctatcgtgg   tgatatgtgt   35160   atctatcgcg   atctgtattt   tattcagcgc   tcattaccta   ctaaggtgat   gaactacaaa   35220   tttaaaactg   gtgaaataga   aattattgat   gctttctaca   accctgacgg   ctcaactggt   35280   caattaccat   aaatttggcg   cttatctaag   ttgtacttgc   tctgaccgac   acaaataatg   35340   tcgtttctca   gcatatatca   aaatacacag   caaaaatttg   gggttagcta   tatagctaac   35400   cccaaatcat   atctaacttt   acactgcatc   taattccaaa   cagtatccag   ccaaaagcct   35460   aaactattgt   tgactcagcg   ctaaaatatg   cgatgcaaca   aacaagtctt   ggatcgcaat   35520   acctgagcta   tcaaaaatgg   tcacctcatc   agcactttga   cgtcctgttg   cggactcgtt   35580   tatcacctga   ccaatctcaa   ttatcggcgt   atttctgcta   tgttgaaact   caccaataac   35640   aatagattga   gaagcaaagt   cgcaaaacaa   gcgagcatga   ctatataggt   cagttggcaa   35700   ctcttgctta   cccactttat   cagcgcccat   tgcagaaata   tgcgttcctg   cttgtaccca   35760   ctgcgcttca   aataaaggcg   cttgagctgt   ggttgctgtg   ataataatat   ctgcttgttc   35820   acaagcagct   tgtgcatcac   aagcttcggc   attaatgcct   ttttctaata   aacgcttaac   35880   caagttttca   gttttgctag   cactacggcc   aactaccaat   accttagtta   atgaacgaac   35940   cttgctcact   gctagcactt   catattcagc   ctgatgaccg   gtaccaaaaa   cagttaatac   36000   cgtagcatct   tctctcgcga   ggtaactcac   tgctactgca   tcggcagcac   cagtgcggta   36060   agcattaacg   gtagtggcag   caatcaccgn   ctgcaacata   ccggttaatg   gatcgagtaa   36120   aaatacgtta   gtgccgtggc   atggtaaacc   atgtttatgg   ttatcaggcc   aatagctgcc   36180   tgttttccag   ccgacaaggt   ttggcgttga   agccgacttt   aatgagaaca   tttcattaag   36240   gttcgcgccc   tgtgcattaa   ctaccgggaa   caaggttgct   ttatcatcta   cggcagcgac   36300   aaacgcttct   ttaacagcga   tataagccag   ctcatgggag   atgagctttg   atgtttgcgc   36360   ttcagttaaa   tagatcatat   taccacccct   gcactcgatt   ccagatctca   tagccaccat   36420   tatcaccatc   agtatcaaat   acatggtact   gagcgtgcat   tgaagctgtt   gcacaggcgt   36480   ggttcggcaa   aatatgtaga   cgactaccta   ccgggaactg   cgctaaatca   ataacgccgc   36540   catcaactgc   ttcaataatg   ccgtgctctt   gattaacagt   tataacctgt   agacctgata   36600   acacgtgacc   gctgtcgtca   cacactaaac   cataaccaca   atcttttggc   tgctctgcag   36660   tacctctatc   acccgaaaga   gccatccaac   ccgcatcaat   gaaaatccag   tttttatcag   36720   gattatgacc   aataacactg   gtcactaccg   ttgcggcaat   atcagttaac   tgacacacgt   36780   ttagccctgc   catgactaaa   tcgaagaagg   tgtacacacc   cgctctaacc   tcggtgatcc   36840   catcaaggtt   ttgatagctt   tgcgctgttg   gtgttgaacc   aatactaacg   atgtcacatt   36900   gcatacccgc   tgcgcgaatg   cgtcagcagc   ttgtacagcc   gctgcaactt   cattttgcgc   36960   cgcatcaatt   aattgctgtt   tttcaaaaca   ttgatatgac   tcaccagcgt   gagtgagtac   37020   gccgtgaaaa   ctcgctgcgc   cagacgttag   tatctgagca   atttcaatca   acttatcggc   37080   ttccggtgga   ataccaccac   gatggccatc   acaatcaatt   tcaattaatg   ctggtatttg   37140   gcagtcataa   gaaccacaga   aatgatttag   ctgatgcgct   tgctcaacac   tatcaagtaa   37200   aactcttgca   ttaatacctt   ggtccaacat   tttagcaata   cgcggcaact   taccatcggc   37260   aatacctact   gcataaataa   tgtctgtgta   acctttagat   gctaaggcct   cggcctcttt   37320   taccgttgat   acagtgactg   gtgagttttt   agtgggtaat   aaaaactcgg   ctgcttcaag   37380   tgatcttaac   gttttaaaat   gcggtcttag   gtttgcacct   aatccttcaa   ttttttggcg   37440   tagttgactg   aggttattaa   taaatactgg   cttatttaca   tataaaaacg   gtgtatcaat   37500   tgcttgatac   tgactttgct   gagtcgtgga   aagtatttga   gtagatggca   tctttaatat   37560   cctagttcat   caatcaatct   aacaagtttg   atgcctagcc   acagtggctt   gtattcatga   37620   tgctttggaa   aatgcttata   ttcaaagtat   ttgaaagaca   tcaaacttct   tgtttaatgc   37680   tcagtatcca   ccagcacgca   tttattttat   attaactatt   atcaagatat   agattaggtt   37740   caaaccaaat   gattagtact   gaagatctac   gttttatcag   cgtaatcgcc   agtcatcgca   37800   ccttagctga   tgccgctaga   acactaaata   tcacgccacc   atcagtgaca   ttaaggttgc   37860   agcatattga   aaagaaacta   tcgattagcc   tgatc   37895   <210> 2 <211> 831 <212> DNA <213> Shewanella putrefaciens SCRC-2874 (FERM BP-1625) <400> 1 atg gta aga ggc tat ttg cgc gct tta ttg tca caa cat agt gaa ata 48 Met Val Arg Gly Tyr Leu Arg Ala Leu Leu Ser Gln His Ser Glu Ile 1 5 10 15 cgc ccc aat gaa tgg cgc ttt gaa tat ggc gac aaa ggt aag cct aga 96 Arg Pro Asn Glu Trp Arg Phe Glu Tyr Gly Asp Lys Gly Lys Pro Arg 20 25 30 ttg agt gat gcg caa ttt gct caa acc ggg gtc cac ttt aat gtg agt 144 Leu Ser Asp Ala Gln Phe Ala Gln Thr Gly Val His Phe Asn Val Ser 35 40 45 cat agt gga gat tgg cta tta gta ggc att tgc act gct gat aat aaa 192 His Ser Gly Asp Trp Leu Leu Val Gly Ile Cys Thr Ala Asp Asn Lys 50 55 60 ggc gcc agt cag gca agc aag gag gaa act gac tct gct agt att gag 240 Gly Ala Ser Gln Ala Ser Lys Glu Glu Thr Asp Ser Ala Ser Ile Glu 65 70 75 80 ttt ggc gtc gac att gag cgt tgc cgt aac agc acc aat atc cac tct 288 Phe Gly Val Asp Ile Glu Arg Cys Arg Asn Ser Thr Asn Ile His Ser 85 90 95 att ctt agt cat tat ttc tct gaa tca gaa aag cga gcc ttg tta gcg 336 Ile Leu Ser His Tyr Phe Ser Glu Ser Glu Lys Arg Ala Leu Leu Ala 100 105 110 tta cca gag gcc ttg cag cga gac cgc ttt ttt gat ttg tgg gcg ctc 384 Leu Pro Glu Ala Leu Gln Arg Asp Arg Phe Phe Asp Leu Trp Ala Leu 115 120 125 aag gag tct tac att aaa gcg aaa gga ctt ggg ctggg tcg cta 432 Lys Glu Ser Tyr Ile Lys Ala Lys Gly Leu Gly Leu Ala Leu Ser Leu 130 135 140 aaa tct ttt gcg ttt gac ttc tct gca ctg agc gaa act ttt ctt gga 480 Lys Ser Phe Ala Phe Asp Phe Ser Ala Leu Ser Glu Thr Phe Leu Gly 145 150 155 160 gtt aat gca cct aaa agc ttg agc cat tgt gtt gat att tcc gat gct 528 Val Asn Ala Pro Lys Ser Leu Ser His Cys Val Asp Ile Ser Asp Ala 165 170 175 att gcg gat cac aag gtt gag cat caa ctt aat cag cga cag gtt ttg 576 Ile Ala Asp His Lys Val Glu His Gln Leu Asn Gln Arg Gln Val Leu 180 185 190 tta aaa caa gat att ggt ctt gct tta cta gag tcg agt tct aat aag 624 Leu Lys Gln Asp Ile Gly Leu Ala Leu Leu Glu Ser Ser Ser Asn Lys 195 200 205 cct aac gct gag cca caa aag tct ggt tta ggt ttg att gag gct aaa 672 Pro Asn Ala Glu Pro Gln Lys Ser Gly Leu Gly Leu Ile Glu Ala Lys 210 215 220 gaa cag caa atg aac gct gct gat aat tgg cat tgt tta ctg ggc cat 720 Glu Gln Gln Met Asn Ala Ala Asp Asn Trp His Cys Leu Leu Gly His 225 230 235 240 ctt gat gat agt tat cgt ttt gca ctg agt att ggt cag tgt cag caa 768 Leu Asp Asp Ser Tyr Arg Phe Ala Leu Ser Ile Gly Gln Cys Gln Gln 245 250 255 ata agt att gca gca gaa gaa gtg aat ttt aaa gct gtt gtt cga gct 816 Ile Ser Ile Ala Glu Glu Val Asn Phe Lys Ala Val Val Arg Ala 260 265 270 tca gct aag act agc 831 Ser Ala Lys Thr Ser 275 <210> 3 <211> 277 <212> PRT <400> 1 Met Val Arg Gly Tyr Leu Arg Ala Leu Leu Ser Gln His Ser Glu Ile 1 5 10 15 Arg Pro Asn Glu Trp Arg Phe Glu Tyr Gly Asp Lys Gly Lys Pro Arg 20 25 30 Leu Ser Asp Ala Gln Phe Ala Gln Thr Gly Val His Phe Asn Val Ser 35 40 45 His Ser Gly Asp Trp Leu Leu Val Gly Ile Cys Thr Ala Asp Asn Lys 50 55 60 Gly Ala Ser Gln Ala Ser Lys Glu Glu Thr Asp Ser Ala Ser Ile Glu 65 70 75 80 Phe Gly Val Asp Ile Glu Arg Cys Arg Asn Ser Thr Asn Ile His Ser 85 90 95 Ile Leu Ser His Tyr Phe Ser Glu Ser Glu Lys Arg Ala Leu Leu Ala 100 105 110 Leu Pro Glu Ala Leu Gln Arg Asp Arg Phe Phe Asp Leu Trp Ala Leu 115 120 125 Lys Glu Ser Tyr Ile Lys Ala Lys Gly Leu Gly Leu Ala Leu Ser Leu 130 135 140 Lys Ser Phe Ala Phe Asp Phe Ser Ala Leu Ser Glu Thr Phe Leu Gly 145 150 155 160 Val Asn Ala Pro Lys Ser Leu Ser His Cys Val Asp Ile Ser Asp Ala 165 170 175 Ile Ala Asp His Lys Val Glu His Gln Leu Asn Gln Arg Gln Val Leu 180 185 190 Leu Lys Gln Asp Ile Gly Leu Ala Leu Leu Glu Ser Ser Ser Asn Lys 195 200 205 Pro Asn Ala Gl u Pro Gln Lys Ser Gly Leu Gly Leu Ile Glu Ala Lys 210 215 220 Glu Gln Gln Met Asn Ala Ala Asp Asn Trp His Cys Leu Leu Gly His 225 230 235 240 Leu Asp Asp Ser Tyr Arg Phe Ala Leu Ser Ile Gly Gln Cys Gln Gln 245 250 255 Ile Ser Ile Ala Ala Glu Glu Val Asn Phe Lys Ala Val Val Arg Ala 260 265 270 Ser Ala Lys Thr Ser 275 <210> 4 <211> 864 <212> DNA <213> Shewanella putrefaciens SCRC-2874 (FERM BP-1625) <400> 1 atg gca aaa ata aat agt gaa cac ttg gat gaa gct act att act tcg 48 Met Ala Lys Ile Asn Ser Glu His Leu Asp Glu Ala Thr Ile Thr Ser 1 5 10 15 aat aag tgt acg caa aca gag act gag gct cgg cat aga aat gcc act 96 Asn Lys Cys Thr Gln Thr Glu Thr Glu Ala Arg His Arg Asn Ala Thr 20 25 30 aca aca cct gag atg cgc cga ttc ata caa gag tcg gat ctc agt gtt 144 Thr Thr Pro Glu Met Arg Arg Phe Ile Gln Glu Ser Asp Leu Ser Val 35 40 45 agc caa ctg tct aaa ata tta aat atc agt gaa gct acc gta cgt aag 192 Ser Gln Leu Ser Lys Ile Leu Asn Ile Ser Glu Ala Thr Val Arg Lys 50 55 60 tgg cgc aag cgt gac tct gtc gaa aac tgt cct aat acc ccg cac cat 240 Trp Arg Lys Arg Asp Ser Val Glu Asn Cys Pro Asn Thr Pro His His 65 70 75 80 ctc aat acc acg cta acc cct ttg caa gaa tat gtg gtt gtg ggc ctg 288 Leu Asn Thr Thr Leu Thr Pro Leu Gln Glu Tyr Val Val Val Gly Leu 85 90 95 cgt tat caa ttg aaa atg cca tta gac aga ttg ctc aaa gca acc caa 336 Arg Tyr Gln Leu Lys Met Pro Leu Asp Arg Leu Leu Lys Ala Thr Gln 100 105 110 gag ttt atc aat cca aac gtg tcg cgc tca ggt tta gca aga tgt ttg 384 Glu Phe Ile Asn Pro Asn Val Ser Arg Ser Gly Leu Ala Arg Cys Leu 115 120 125 aag cgt tat ggc gtt tca cgg gtg agt gat atc caag cac gta 432 Lys Arg Tyr Gly Val Ser Arg Val Ser Asp Ile Gln Ser Pro His Val 130 135 140 cca atg cgc tac ttt aat caa att cca gtc act caa ggc agc gat gtg 480 Pro Met Arg Tyr Phe Asn Gln Ile Pro Val Thr Gln Gly Ser Asp Val 145 150 155 160 caa acc tac acc ctg cac tat gaa acg ctg gca aaa acc tta gcc tta 528 Gln Thr Tyr Thr Leu His Tyr Glu Thr Leu Ala Lys Thr Leu Ala Leu 165 170 175 cct agt acc gat ggt gac aat gtg gtg caa gtg gtg tct ctc acc att 576 Pro Ser Thr Asp Gly Asp Asn Val Val Gln Val Val Ser Leu Thr Ile 180 185 190 cca cca aag tta acc gaa gaa gca ccc agt tca att ttg ctc ggc att 624 Pro Pro Lys Leu Thr Glu Glu Ala Pro Ser Ser Ile Leu Leu Gly Ile 195 200 205 gat cct cat agc gac tgg atc tat ctc gac ata tac caa gat ggc aat 672 Asp Pro His Ser Asp Trp Ile Tyr Leu Asp Ile Tyr Gln Asp Gly Asn 210 215 220 aca caa gcc acg aat aga tat atg gct tat gtg cta aaa cac ggg cca 720 Thr Gln Ala Thr Asn Arg Tyr Met Ala Tyr Val Leu Lys His Gly Pro 225 230 235 240 ttc cat tta cga aag tta ctc gtg cgt aac tat cac acc ttt tta cag 768 Phe His Leu Arg Lys Leu Leu Val Arg Asn Tyr His Thr Phe Leu Gln 245 250 255 cgc ttt cct gga gcg acg caa aat cgc cgc ccc tct aaa gat atg cct 816 Arg Phe Pro Gly Ala Thr Gln Asn Arg Arg Pro Ser Lys Asp Met Pro 260 265 270 270 gaa aca atc aac aag acg cct gaa aca cag gca ccc agt gga gac tca 864 Glu Thr Ile Asn Lys Thr Pro Glu Thr Gln Ala Pro Ser Gly Asp Ser 275 280 285 <210> 5 <211> 288 <212> PRT <400> 1 Met Ala Lys Ile Asn Ser Glu His Leu Asp Glu Ala Thr Ile Thr Ser 1 5 10 15 Asn Lys Cys Thr Gln Thr Glu Thr Glu Ala Arg His Arg Asn Ala Thr 20 25 30 Thr Thr Pro Glu Met Arg Arg Phe Ile Gln Glu Ser Asp Leu Ser Val 35 40 45 Ser Gln Leu Ser Lys Ile Leu Asn Ile Ser Glu Ala Thr Val Arg Lys 50 55 60 Trp Arg Lys Arg Asp Ser Val Glu Asn Cys Pro Asn Thr Pro His His 65 70 75 80 Leu Asn Thr Thr Leu Thr Pro Leu Gln Glu Tyr Val Val Val Gly Leu 85 90 95 Arg Tyr Gln Leu Lys Met Pro Leu Asp Arg Leu Leu Lys Ala Thr Gln 100 105 110 Glu Phe Ile Asn Pro Asn Val Ser Arg Ser Gly Leu Ala Arg Cys Leu 115 120 125 Lys Arg Tyr Gly Val Ser Arg Val Ser Asp Ile Gln Ser Pro His Val 130 135 140 Pro Met Arg Tyr Phe Asn Gln Ile Pro Val Thr Gln Gly Ser Asp Val 145 150 155 160 Gln Thr Tyr Thr Leu His Tyr Glu Thr Leu Ala Lys Thr Leu Ala Leu 165 170 175 Pro Ser Thr Asp Gly Asp Asn Val Val Gln Val Val Ser Leu Thr Ile 180 185 190 Pro Pro Lys Leu Thr Glu Glu Ala Pro Ser Ser Ile Leu Leu Gly Ile 195 200 205 Asp Pro His S er Asp Trp Ile Tyr Leu Asp Ile Tyr Gln Asp Gly Asn 210 215 220 Thr Gln Ala Thr Asn Arg Tyr Met Ala Tyr Val Leu Lys His Gly Pro 225 230 235 240 Phe His Leu Arg Lys Leu Leu Val Arg Asn Tyr His Thr Phe Leu Gln 245 250 255 Arg Phe Pro Gly Ala Thr Gln Asn Arg Arg Pro Ser Lys Asp Met Pro 260 265 270 Glu Thr Ile Asn Lys Thr Pro Glu Thr Gln Ala Pro Ser Gly Asp Ser 275 280 285 <210> 6 <211> 8268 <212> DNA <213> Shewanella putrefaciens SCRC-2874 (FERM BP-1625) <400> 1 atg agc cag acc tct aaa cct aca aac tca gca act gag caa gca caa 48 Met Ser Gln Thr Ser Lys Pro Thr Asn Ser Ala Thr Glu Gln Ala Gln 1 5 10 15 gac tca caa gct gac tct cgt tta aat aaa cga cta aaa gat atg cca 96 Asp Ser Gln Ala Asp Ser Arg Leu Asn Lys Arg Leu Lys Asp Met Pro 20 25 30 att gct att gtt ggc atg gcg agt att ttt gca aac tct cgc tat ttg 144 Ile Ala Ile Val Gly Met Ala Ser Ile Phe Ala Asn Ser Arg Tyr Leu 35 40 45 aat aag ttt tgg gac tta atc agc gaa aaa att gat gcg att act gaa 192 Asn Lys Phe Trp Asp Leu Ile Ser Glu Lys Ile Asp Ala Ile Thr Glu 50 55 60 tta cca tca act cac tgg cag cct gaa gaa tat tac gac gca gat aaa 240 Leu Pro Ser Thr His Trp Gln Pro Glu Glu Tyr Tyr Asp Ala Asp Lys 65 70 75 80 acc gca gca gac aaa agc tac tgt aaa cgt ggt ggc ttt ttg cca gat 288 Thr Ala Ala Asp Lys Ser Tyr Cys Lys Arg Gly Gly Phe Leu Pro Asp 85 90 95 gta gac ttc aac cca atg gag ttt ggc ctg ccg cca aac att ttg gaa 336 Val Asp Phe Asn Pro Met Glu Phe Gly Leu Pro Pro Asn Ile Leu Glu 100 105 110 ctg acc gat tca tcg caa cta tta tca ctc atc gtt gct aaa gaa gtg 384 Leu Thr Asp Ser Ser Gln Leu Leu Ser Leu Ile Val Ala Lys Glu Val 115 120 125 ttg gct gat gct aac tta cct gag aat tac gac cgc gat aaa att ggt 432 Leu Ala Asp Ala Asn Leu Pro Glu Asn Tyr Asp Arg Asp Lys Ile Gly 130 135 140 atc acc tta ggt gtc ggc ggt ggt caa aaa att agc cac agc cta aca 480 Ile Thr Leu Gly Val Gly Gly Gly Gln Lys Ile Ser His Ser Leu Thr 145 150 155 160 gcg cgt ctg caa tac cca gta ttg aag aaa gta ttc gcc aat agc ggc 528 Ala Arg Leu Gln Tyr Pro Val Leu Lys Lys Val Phe Ala Asn Ser Gly 165 170 175 att agt gac acc gac agc gaa atg ctt atc aag aaa ttc caa gac caa 576 Ile Ser Asp Thr Asp Ser Glu Met Leu Ile Lys Lys Phe Gln Asp Gln 180 185 190 tat gta cac tgg gaa gaa aac tcg ttc cca ggt tca ctt ggt ayr gtt 624 His Trp Glu Glu Asn Ser Phe Pro Gly Ser Leu Gly Asn Val 195 200 205 att gcg ggc cgt atc gcc aac cgc ttc gat ttt ggc ggc atg aac tgt 672 Ile Ala Gly Arg Ile Ala Asn Arg Phe Asp Phe Gly Gly Met Asn210 215 220 gtg gtt gat gct gcc tgt gct gga tca ctt gct gct atg cgt atg gcg 720 Val Val Asp Ala Ala Cys Ala Gly Ser Leu Ala Ala Met Arg Met Ala 225 230 235 240 cta aca gag cta act gaa ggt cgc tct gaa atg atg atc acc ggt ggt 768 Leu Thr Glu Leu Thr Glu Gly Arg Ser Glu Met Met Ile Thr Gly Gly 245 250 255 gtg tgt act gat aac tca ccc tct atg tat atg agc ttt tca aaa acg 816 Val Cys Thr Asp Asn Ser Pro Ser Met Tyr Met Ser Phe Ser Lys Thr 260 265 270 ccc gcc ttt acc act aac gaa acc att cag cca ttt gat atc gac tca 864 Pro Ala Phe Thr Thr Asn Glu Thr Ile Gln Pro Phe Asp Ile Asp Ser 275 280 285 aaa ggc atg atg att ggt gaa ggt att ggc atg gtg gcg cta aag cgt 912 Lys Gly Met Met Ile Gly Glu Gly Ile Gly Met Val Ala Leu Lys Arg 290 295 300 ctt gaa gat gca gag cgc gat ggc attac ttac 960 Leu Glu Asp Ala Glu Arg Asp Gly Asp Arg Ile Tyr Ser Val Ile Lys 305 310 315 320 ggt gtg ggt gca tca tct gac ggt aag ttt aaa tca atc tat gcc cct 1008 Gly Val Gly Ala Ser Ser Asp Gly Lys Phe Lys Ser Ile Tyr Ala Pro 325 330 335 cgc cca tca ggc caa gct aaa gca ctt aac cgt gcc tat gat gac gca 1056 Arg Pro Ser Gly Gln Ala Lys Ala Leu Asn Arg Ala Tyr Asp Asp Ala 340 345 350 ggt ttt gcg ccg cat acctta ggt cta att gaa gct cac gga aca ggt 1104 Gly Phe Ala Pro His Thr Leu Gly Leu Ile Glu Ala His Gly Thr Gly 355 360 365 act gca gca ggt gac gcg gca gag ttt gcc ggc ctt tgc tca gta ttt 1152 Thr Ala Ala Gly Asp Ala Ala Glu Phe Ala Gly Leu Cys Ser Val Phe 370 375 380 gct gaa ggc aac gat acc aag caa cac att gcg cta ggt tca gtt aaa 1200 Ala Glu Gly Asn Asp Thrys Lys Gln His Ile Ala Leu Gly Ser Val Lys 385 390 395 400 tca caa att ggt cat act aaa tca act gca ggt aca gca ggt tta att 1248 Ser Gln Ile Gly His Thr Lys Ser Thr Ala Gly Thr Ala Gly Leu Ile 405 410 415 aaa gct gct ctt gct ttg cat cac aag gta ctg ccg ccg acc att aac 1296 Lys Ala Ala Leu Ala Leu His His Lys Val Leu Pro Pro Thr Ile Asn 420 425 430 gtt agt cag cca agc cct aaa ctt gat atc gaa aac tca ccg ttt tat 1344 Val Ser Gln Pro Ser Pro Lys Leu Asp Ile Glu Asn Ser Pro Phe Tyr 435 440 445 cta aac act gag act cgt cca tgg tta cca cgt gtt gat ggt acg ccg 1392 Leu Asn Thr Glu Thr Arg Pro Trp Leu Pro Arg Val Asp Gly Thr Pro 450 455 460 cgc cgc gcg ggt att agc tca ttt ggt ttt ggt ggc act aac ttc cat 1440 Arg Arg Ala Gly Ile Ser Ser Phe Gly Phe Gly Gly Thr Asn Phe His 465 470 475 475 480 ttt gta cta gaa gag tac aac caa gaa cac agc cgt act gat gaa 1488 Phe Val Leu Glu Glu Tyr Asn Gln Glu His Ser Arg Thr Asp Ser Glu 485 490 aaa aaa gct aag tat cgt caa cgc caa gtg gcg caa agc ttc ctt gtt agc 1536 Lys Ala Lys Tyr Arg Gln Arg Gln Val Ala Phe Leu Val Ser 500 505 510 gca agc gat aaa gca tcg cta att aac gag tta aac gta cta gca gca 1584 Ala Ser Asp Lys Ala Ser Leu Ile Asn Glu Leu Asn Val Leu Ala Ala 515 520 525 tct gca agc caa gct gag ttt atc ctc aaa gat gca gca gca aac tat 1632 Ser Ala Ser Gln Ala Glu Phe Ile Leu Lys Asp Ala Ala Ala Asn Tyr 530 535 540 ggc gta cgt gag ctt gat aaa aat gca cca cgg atc ggt tta gtt gca 1680 Gly Val Arg Glu Leu Asp Lys Asn Ala Pro Arg Ile Gly Leu Val Ala 545 550 555 560 aac aca gct gaa gag tta gca ggc cta att aag caa gca ctt gcc aaa 1728 Asn Thr Ala Glu Glu Leu Ala Gly Leu Ile Lys Gln Ala Le A Lys 565 570 575 cta gca gct agc gat gat aac gca tgg cag cta cct ggt ggc act agc 1776 Leu Ala Ala Ser Asp Asp Asn Ala Trp Gln Leu Pro Gly Gly Thr Ser 580 585 590 tac cgc gcc gct gca gta gatt ggt gcc gca ctg ttt gct ggc 1824 Tyr Arg Ala Ala Ala Val Glu Gly Lys Val Ala Ala Leu Phe Ala Gly 595 600 605 caa ggt tca caa tat ctc aat atg ggc cgt gac ctt act tgt tat tac 1872 Gln Gly Ser Gln Tyr Le Met Gly Arg Asp Leu Thr Cys Tyr Tyr 610 615 620 cca gag atg cgt cag caa ttt gta act gca gat aaa gta ttt gcc gca 1920 Pro Glu Met Arg Gln Gln Phe Val Thr Ala Asp Lys Val Phe Ala Ala 625 630 630 635 640 aat gat aaa acg ccg tta tcg caa act ctg tat cca aag cct gta ttt 1968 Asn Asp Lys Thr Pro Leu Ser Gln Thr Leu Tyr Pro Lys Pro Val Phe 645 650 655 aat aaa gat gaa tta aag gct caa gaa gcc att ttg acc a at acc gcc 2016 Asn Lys Asp Glu Leu Lys Ala Gln Glu Ala Ile Leu Thr Asn Thr Ala 660 665 670 aat gcc caa agc gca att ggt gcg att tca atg ggt caa tac gat ttg 2064 Asn Ala Gln Ser Ala Ile Gly Ala Ile Ser Met Gly Gln Tyr Asp Leu 675 680 685 ttt act gcg gct ggc ttt aat gcc gac atg gtt gca ggc cat agc ttt 2112 Phe Thr Ala Ala Gly Phe Asn Ala Asp Met Val Ala Gly His Ser Phe 690 695 700 ggt gag cta agt gca ctg tgt gct gca ggt gtt att tca gct gat gac 2160 Gly Glu Leu Ser Ala Leu Cys Ala Ala Gly Val Ile Ser Ala Asp Asp 705 710 715 720 tac tac aag ctg gct ttt gct cgt ggt gag gct atg gca aca aaa gca 2208yr Tyr Lys Leu Ala Phe Ala Arg Gly Glu Ala Met Ala Thr Lys Ala 725 730 735 ccg gct aaa gac ggc gtt gaa gca gat gca gga gca atg ttt gca atc 2256 Pro Ala Lys Asp Gly Val Glu Ala Asp Ala Gly Ala Met Phe Ile 740 745 750 ata acc aag agt gct gca gac ctt gaa acc gtt gaa gcc acc atc gct 2304 Ile Thr Lys Ser Ala Ala Asp Leu Glu Thr Val Glu Ala Thr Ile Ala 755 760 765 aaa ttt gat ggg gtg aaa gtc gct aa c tat aac gcg cca acg caa tca 2352 Lys Phe Asp Gly Val Lys Val Ala Asn Tyr Asn Ala Pro Thr Gln Ser 770 775 780 gta att gca ggc cca aca gca act acc gct gat gcg gct aaa gcg cta 2400 Val Ile Ala Gly Pro Thr Ala Thr Thr Ala Asp Ala Ala Lys Ala Leu 785 790 795 800 act gag ctt ggt tac aaa gcg att aac ctg cca gta tca ggt gca ttc 2448 Thr Glu Leu Gly Tyr Lys Ala Ile Asn Leu Pro Val Ser Gly Ala Phe 805 810 815 cac act gaa ctt gtt ggt cac gct caa gcg cca ttt gct aaa gcg att 2496 His Thr Glu Leu Val Gly His Ala Gln Ala Pro Phe Ala Lys Ala Ile 820 825 830 gac gca gcc aaa ttt act aaa aca agc cga gca cttac tca aat gca 2544 Asp Ala Ala Lys Phe Thr Lys Thr Ser Arg Ala Leu Tyr Ser Asn Ala 835 840 845 act ggc gga ctt tat gaa agc act gct gca aag att aaa gcc tcg ttt 2592 Thr Gly Gly Leu Tyr Glu Ser Thr Ala Ala Lys Ile Lys Ala Ser Phe 850 855 860 aag aaa cat atg ctt caa tca gtg cgc ttt act agc cag cta gaa gcc 2640 Lys Lys His Met Leu Gln Ser Val Arg Phe Thr Ser Gln Leu Glu Ala 865 870 875 875 880 atg tac aac gac ggc gcc cgt gta ttt gtt gaa ttt ggt cca aag aac 2688 Met Tyr Asn Asp Gly Ala Arg Val Phe Val Glu Phe Gly Pro Lys Asn 885 890 895 atc tta caa aaa tta gtt caa ggc acg ctt gtc aac gaa aat Ile Leu Gln Lys Leu Val Gln Gly Thr Leu Val Asn Thr Glu Asn Glu 900 905 910 gtt tgc act atc tct atc aac cct aat cct aaa gtt gat agt gat ctg 2784 Val Cys Thr Ile Ser Ile Asn Pro Asn Pro Lys Val Asp Ser Asp Leu 915 920 925 cag ctt aag caa gca gca atg cag cta gcg gtt act ggt gtg gta ctc 2832 Gln Leu Lys Gln Ala Ala Met Gln Leu Ala Val Thr Gly Val Val Leu 930 935 940 agt gaa att gac cca tac caa gcc att gcc gca cca gcg aaa aag 2880 Ser Glu Ile Asp Pro Tyr Gln Ala Asp Ile Ala Ala Pro Ala Lys Lys 945 950 955 960 tcg cca atg agc att tcg ctt aat gct gct aac cat atc agc aaa gca 2928 Ser Pro Met Ser Ser Leu Asn Ala Ala Asn His Ile Ser Lys Ala 965 970 975 act cgc gct aag atg gcc aag tct tta gag aca ggt atc gtc acc tcg 2976 Thr Arg Ala Lys Met Ala Lys Ser Leu Glu Thr Gly Ile Val Thr Ser 980 985 990 caa ata gaa cat gtt att gaa gaa aaa atc gtt gaa gtt gag aaa ctg 3024 Gln Ile Glu His Val Ile Glu Glu Lys Ile Val Glu Val Glu Lys Leu 995 1000 1005 gtt gaa gtc gaa aag atc gtc gga aaa gta gag aaa gtt 3072 Val Glu Val Glu Lys Ile Val Glu Lys Val Val Glu Val Glu Lys Val 1010 1015 1020 gtt gag gtt gaa gct cct gtt aat tca gtg caa gcc aat gca att caa 3120 Val Glu Val Glu Ala Pro Val Asn Ser Val Gln Ala Asn Ala Ile Gln 1025 1030 1035 1040 acc cgt tca gtt gtc gct cca gta ata gag aac caa gtc gtg tct aaa 3168 Thr Arg Ser Val Val Ala Pro Val Ile Glu Asn Gln Val Val Ser Lys 1045 1050 1055 aac agt aag cca gca gtc cag agc att agt ggt gat gca ctc agc aac 3216 Asn Ser Lys Pro Ala Val Gln Ser Ile Ser Gly Asp Ala Leu Ser Asn 1060 1065 1070 ttt ttt gct gca cag cag caa acc gca cag ttg cat cag cag ttc tta 3264 Phe Phe Ala Ala Gln Gln Gln Thr Ala Gln Leu His Gln Gln Phe Leu 1075 1080 1085 gct att ccg cag caa tat ggt gag acg ttc act acg ctg atg acc gag 3312 Ala Ile Pro Gln Gln Tyr Gly Glu Thr Phe Thr Thr Leu Met Thr Glu 1090 1095 1100 caa gct aaa ctg gca agt tct ggt gtt gca att cca gag agt ctg caa 3360 Gln Ala Lys Leu Ala Ser Ser Gly Val Ala Ile Pro Glu Ser Leu Gln 1105 1110 1115 1120 cgc tca atg gag caa ttc cac caa cta caa gcg caa aca cta caa agc 3408 Arg Ser Met Glu Gln Phe His Gln Leu Gln Ala Gln Thr Leu Gln Ser 1125 1130 1135 cac acc cag ttc ctt gag atg caa gcg ggt agc aac att gca 3456 His Thr Gln Phe Leu Glu Met Gln Ala Gly Ser Asn Ile Ala Ala Leu 1140 1145 1150 aac cta ctc aat agc agc caa gca act tac gct cca gcc att cac aat 3504 Asn Leu Leu Asn Ser Ser Gln Ala Thr Tyr Ala Pro Ala Ile His Asn 1155 1160 1165 gaa gcg att caa agc caa gtg gtt caa agc caa act gca gtc cag cca 3552 Glu Ala Ile Gln Ser Gln Val Val Gln Ser Gln Thr Ala Val Gln Pro 1170 1175 1180 gta att tca aca caa gtt aac cat gtg tca gag cag cca act caa gct 3600 Val Ile Ser Thr Gln Val Asn His Val Ser Glu Gln Pro Thr Gln Ala 1185 1190 1195 1200 cca gct cca aaa gcg cag cca gca cct gtg aca act cca g tt caa act 3648 Pro Ala Pro Lys Ala Gln Pro Ala Pro Val Thr Thr Pro Val Gln Thr 1205 1210 1215 gct ccg gca caa gtt gtt cgt caa gcc gca cca gtt caa gcc gct att 3696 Ala Pro Ala Gln Val Val Arg Gln Ala Ala Pro Val Gln Ala Ala Ile 1220 1225 1230 gaa ccg att aat aca agt gtt gcg act aca acg cct tca gcc ttc agc 3744 Glu Pro Ile Asn Thr Ser Val Ala Thr Thr Thr Thr Pro Ser Ala Phe Ser 1235 1240 1245 gcc gaa aca gcc ctg agc gca aca aaa gtc caa gcc act atg ctt gaa 3792 Ala Glu Thr Ala Leu Ser Ala Thr Lys Val Gln Ala Thr Met Leu Glu 1250 1255 1260 gtg gtt gct gag aaa acc ggt tac cca act gaa atg cta gag ctt gaa 3840 Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu Met Leu Glu Leu Glu 1265 1270 1275 1280 atg gat atg gaa gcc gat tta ggc atc gat tct atc aag cgt gta gaa 3888 Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Glu 1285 1290 1295 att ctt ggc aca gta caa gat gag cta ccg ggt cta cct gag ctt agc 3936 Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly Leu Pro Glu Leu Ser 1300 1305 1310 cct gaga gat cta gct gag tgt cga acg cta ggc gaa atc gtt gac tat 3984 Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu Ile Val Asp Tyr 1315 1320 1325 atg ggc agt aaa ctg ccg gct gaa ggc tct 40 atg aat tct ca Met Gly Ser Lys Leu Pro Ala Glu Gly Ser Met Asn Ser Gln Leu Ser 1330 1335 1340 aca ggt tcc gca gct gcg act cct gca gcg aat ggt ctt tct gcg gag 4080 Thr Gly Ser Ala Ala Ala Thr Pro Ala Ala Asn Gly Leu Ser Ala Glu 1345 1350 1355 1360 aaa gtt caa gcg act atg atg tct gtg gtt gcc gaa aag act ggc tac 4128 Lys Val Gln Ala Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr 1365 1370 1375 cca act gaa atg cta gag ctt gaa atg gat atg gaa gcc gat tta ggc 4176 Pro Thr Glu Met Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly 1380 1385 1390 ata gat tct atc aag cgc gtt gaa att ctt ggc aca gta caa gat gag 4224 Ile Asp Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu 1395 1400 1405 cta ccg ggt cta cct gag ctt agc cct gaa gat cta gct gag tgt cgt 4272 Leu Pro Gly Leu Pro Glu Leu Ser Pro Glu Asp Leu A la Glu Cys Arg 1410 1415 1420 act cta ggc gaa atc gtt gac tat atg aac tct aaa ctc gct gac ggc 4320 Thr Leu Gly Glu Ile Val Asp Tyr Met Asn Ser Lys Leu Ala Asp Gly 1425 1430 1435 1440 tct aag ctggag ggc tct atg aat tct cag ctg tct aca agt 4368 Ser Lys Leu Pro Ala Glu Gly Ser Met Asn Ser Gln Leu Ser Thr Ser 1445 1450 1455 gcc gca gct gcg act cct gca gcg aat ggt ctc tct gcg gag aaa gtt 4416 Ala Ala Ala Ala Thr Pro Ala Ala Asn Gly Leu Ser Ala Glu Lys Val 1460 1465 1470 caa gcg act atg atg tct gtg gtt gcc gaa aag act ggc tac cca act 4464 Gln Ala Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr Pro Thr 1475 1480 1485 gaa atg cta gaa ctt gaa atg gat atg gaa gct gac ctt ggc atc gat 4512 Glu Met Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly Ile Asp 1490 1495 1500 tca atc aag cgc gtt gaa att g gat gag cta ccg 4560 Ser Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro 1505 1510 1515 1520 ggt tta cct gag cta aat cca gaa gat ttg gca gag tgt cgt act ctt 4608 Gly Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu 1525 1530 1535 ggc gaa atc gtg act tat atg aac tct aaa ctc gct gac ggc tct aag 4656 Gly Glu Ile Val Thr Tyr Met Asn Ser Lys Leu Ala Asp Gly Lys 1540 1545 1550 ctg cca gct gaa ggc tct atg cac tat cag ctg tct aca agt acc gct 4704 Leu Pro Ala Glu Gly Ser Met His Tyr Gln Leu Ser Thr Ser Thr Ala 1555 1560 1565 gct gcg act cct gta gcg aat ggt ctc tct gca gaa aaa gtt caa gcg 4752 Ala Ala Thr Pro Val Ala Asn Gly Leu Ser Ala Glu Lys Val Gln Ala 1570 1575 1580 acc atg atg tct gta gtt gca gat aaa act ggc tac cca act gaa atg 4800 Thr Met Met Ser Val Val Ala Asp Lys Thr Gly Tyr Pro Thr Glu Met 1585 1590 1595 1600 ctt gaa ctt gaa atg gat atg gaa gcc gat tta ggt atc gat tct atc 4848 Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile 1605 1610 a cgc gtt gaa att ctt ggc aca gta caa gat gag cta ccg ggt tta 4896 Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly Leu 1620 1625 1630 cct gag cta aat cca gaa gat cta gca gag tgt cgc acc cta ggc gaa 4944 Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu 1635 1640 1645 atc gtt gac tat atg ggc agt aaa ctg ccg gct gaa ggc tct gct aat Tap Ast 4992yr Val Gly Ser Lys Leu Pro Ala Glu Gly Ser Ala Asn 1650 1655 1660 aca agt gcc gct gcg tct ctt aat gtt agt gcc gtt gcg gcg cct caa 5040 Thr Ser Ala Ala Ala Ala Ser Leu Asn Val Ser Ala Val Ala Ala Pro Gln 1665 1670 1675 1680 gct gct gcg act cct gta tcg aac ggt ctc tct gca gag aaa gtg caa 5088 Ala Ala Ala Thr Pro Val Ser Asn Gly Leu Ser Ala Glu Lys Val Gln 1685 1690 1695 agc act atg atg tca gta gtt gca gaa aag acc ggc tac cca act gaa 5136 Ser Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu 1700 1705 1710 atg cta gaa ctt ggc atg gat atg gaa gcc gat tta ggt atc gac tca 5184 Met Leu Glu Leu Gly Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser 1715 1720 1725 att aaa cgc gtt gag att ctt ggc aca gta caa gat gag cta ccg ggt 5232 Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly 1730 1735 1740 cta cca gag ctt aat cct gaa gat tta gct gag tgc cgt acg ctg ggc 5280 Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly 1745 1750 1755 1760 gaa atc gtt gac tat atg act gct ag tct gac ggc tct aag ctt 5328 Glu Ile Val Asp Tyr Met Asn Ser Lys Leu Ala Asp Gly Ser Lys Leu 1765 1770 1775 cca gct gaa ggc tct gct aat aca agt gcc act gct gcg act cct gca 5376 Pro Ala Glu Gly Ser Ala Asn Thr Ser Ala Thr Ala Ala Thr Pro Ala 1780 1785 1790 gtg aat ggt ctt tct gct gac aag gta cag gcg act atg atg tct gta 5424 Val Asn Gly Leu Ser Ala Asp Lys Val Gln Ala Thr Met Met Ser Val 1795 1800 1805 gtt gct gaa aag acc ggc tac cca act gaa atg cta gaa ctt ggc atg 5472 Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu Met Leu Glu Leu Gly Met 1810 1815 1820 gat atg gaa gca gac ctt ggt att gat tct att aag cgc gtt gtt ata Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile 1825 1830 1835 1840 ctt ggc aca gta caa gat gag ctc cca ggt tta cct gag ctt aat cct 5568 Leu Gly Thr Val Val Gln Asp Glu Leu Pro Gly Leu Pro Glu Leu Asn Pro 1845 1850 1855 gaa gat ctc gct gag tgc cgc acg ctt ggc gaa atc gtt agc tat atg 5616 Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu Ile Val Ser Tyr Met 1860 1870 tct caa ctg gct gat ggc tct aaa ctt tct aca agt gcg gct gaa 5664 Asn Ser Gln Leu Ala Asp Gly Ser Lys Leu Ser Thr Ser Ala Ala Glu 1875 1880 1885 ggc tct gct gat aca agt gct gca aat gct gca aagcag g att 5712 Gly Ser Ala Asp Thr Ser Ala Ala Asn Ala Ala Lys Pro Ala Ala Ile 1890 1895 1900 tcg gca gaa cca agt gtt gag ctt cct cct cat agc gag gta gcg cta 5760 Ser Ala Glu Pro Ser Val Glu Leu Pro Pro His Ser Glu Val Ala Leu 1905 1910 1915 1920 aaa aag ctt aat gcg gcg aac aag cta gaa aat tgt ttc gcc gca gac 5808 Lys Lys Leu Asn Ala Ala Asn Lys Leu Glu Asn Cys Phe Ala Ala Asp 1925 1930 1935 gca ag tgt gat gat ggt cac aac gca ggc gtt tta gct 5856 Ala Ser Val Val Ile Asn Asp Asp Gly His Asn Ala Gly Val Leu Ala 1940 1945 1950 gag aaa ctt att aaa caa ggc cta aaa gta gcc gtt gt g cgt tta ccg 5904 Glu Lys Leu Ile Lys Gln Gly Leu Lys Val Ala Val Val Arg Leu Pro 1955 1960 1965 aaa ggt cag cct caa tcg cca ctt tca agc gat gtt gct agc ttt gag 5952 Lys Gly Gln Pro Gln Ser Pro Leu Ser Ser Asp Val Ala Ser Phe Glu 1970 1975 1980 ctt gcc tca agc caa gaa tct gag ctt gaa gcc agt atc act gca gtt 6000 Leu Ala Ser Ser Gln Glu Ser Glu Leu Glu Ala Ser Ile Throra Ala Val 1985 1990 1995 2000 atc gcg cag att gaa act cag gtt ggc gct att ggt ggc ttt att cac 6048 Ile Ala Gln Ile Glu Thr Gln Val Gly Ala Ile Gly Gly Phe Ile His 2005 2010 2015 ttg caa cca gaa gcg aat aca gaa gag caa acg gca gta a96 cta gat Leu Gln Pro Glu Ala Asn Thr Glu Glu Gln Thr Ala Val Asn Leu Asp 2020 2025 2030 gcg caa agt ttt act cac gtt agc aat gcg ttc ttg tgg gcc aaa tta 6144 Ala Gln Ser Phe Thr His Val Ser Asn Ala Phe Leu Trp Ala Lys Leu 2035 2040 2045 ttg caa cca aag ctc gtt gct gga gca gat gcg cgt cgc tgt ttt gta 6192 Leu Gln Pro Lys Leu Val Ala Gly Ala Asp Ala Arg Arg Cys Phe Val 2050 2055 2060 aca gta a gc cgt atc gac ggt ggc ttt ggt tac cta aat act gac gcc 6240 Thr Val Ser Arg Ile Asp Gly Gly Phe Gly Tyr Leu Asn Thr Asp Ala 2065 2070 2075 2080 cta aaa gat gct gag cta aac caa gca gca tta gct ggt tta act aaa 6288 Leu Lys Asp Ala Glu Leu Asn Gln Ala Ala Leu Ala Gly Leu Thr Lys 2085 2090 2095 acc tta agc cat gaa tgg cca caa gtg ttc tgt cgc gcg cta gat att 6336 Thr Leu Ser His Glu Trp Pro Gln Val Phe Cys Ar Ala Leu Asp Ile 2100 2105 2110 gca aca gat gtt gat gca acc cat ctt gct gat gca atc acc agt gaa 6384 Ala Thr Asp Val Asp Ala Thr His Leu Ala Asp Ala Ile Thr Ser Glu 2115 2120 2125 cta ttt gat agc caa gct cag cta cct gaa gtg ggc tta agc tta att 6432 Leu Phe Asp Ser Gln Ala Gln Leu Pro Glu Val Gly Leu Ser Leu Ile 2130 2135 2140 gat ggc aaa gtt aac cgc gta act cta gtt gct gct gaa gct gca gat Lys Asn Arg Val Thr Leu Val Ala Ala Glu Ala Ala Asp 2145 2150 2155 2160 aaa aca gca aaa gca gag ctt aac agc aca gat aaa atc tta gtg act 6528 Lys Thr Ala Lys Ala Glu Leu Asn Ser Thr As p Lys Ile Leu Val Thr 2165 2170 2175 ggt ggg gca aaa ggg gtg aca ttt gaa tgt gca ctg gca tta gca tct 6576 Gly Gly Ala Lys Gly Val Thr Phe Glu Cys Ala Leu Ala Leu Ala Ser 2180 2185 2190 cgc agc cag tc ttt atc tta gct ggg cgc agt gaa tta caa gct 6624 Arg Ser Gln Ser His Phe Ile Leu Ala Gly Arg Ser Glu Leu Gln Ala 2195 2200 2205 tta cca agc tgg gct gag ggt aag caa act agc gag cta aaa tca gct 6672u Ser Trp Ala Glu Gly Lys Gln Thr Ser Glu Leu Lys Ser Ala 2210 2215 2220 gca atc gca cat att att tct act ggt caa aag cca acg cct aag caa 6720 Ala Ile Ala His Ile Ile Ser Thr Gly Gln Lys Pro Thr Pro Lys Gln 2225 2230 2235 2240 gtt gaa gcc gct gtg tgg cca gtg caa agc agc att gaa att aat gcc 6768 Val Glu Ala Ala Val Trp Pro Val Gln Ser Ser Ile Glu Ile Asn Ala 2245 2250 2255 gcc cta gcc gcc ttt ac gcc gtt gg tca gct gaa tac gtc agc 6816 Ala Leu Ala Ala Phe Asn Lys Val Gly Ala Ser Ala Glu Tyr Val Ser 2260 2265 2270 atg gat gtt acc gat agc gcc gca atc aca gca gca ctt aat ggt cgc 6864 Met Asp Val Thr Asp Ser Ala Ala Ile Thr Ala Ala Leu Asn Gly Arg 2275 2280 2285 tca aat gag atc acc ggt ctt att cat ggc gca ggt gta cta gcc gac 6912 Ser Asn Glu Ile Thr Gly Leu Ile His Gly Ala Gly Val Leu Ala Asp 2290 2295 2300 aag cat att caa gac aag act ctt gct gaa ctt gct aaa gtt tat ggc 6960 Lys His Ile Gln Asp Lys Thr Leu Ala Glu Leu Ala Lys Val Tyr Gly 2305 2310 2315 2320 act aaa gtc aac ggc cta aaa g ctg ctc gcg gca ctt gag cca agc 7008 Thr Lys Val Asn Gly Leu Lys Ala Leu Leu Ala Ala Leu Glu Pro Ser 2325 2330 2335 aaa att aaa tta ctt gct atg ttc tca tct gca gca ggt ttt tac ggt 7056 Lys Leu Lys Ala Met Phe Ser Ser Ala Ala Gly Phe Tyr Gly 2340 2345 2350 aat atc ggc caa agc gat tac gcg atg tcg aac gat att ctt aac aag 7104 Asn Ile Gly Gln Ser Asp Tyr Ala Met Ser Asn Asp Ile Leu Asn Lys 2355 2360 gca gcg ctg cag ttc acc gct cgc aac cca caa gct aaa gtc atg agc 7152 Ala Ala Leu Gln Phe Thr Ala Arg Asn Pro Gln Ala Lys Val Met Ser 2370 2375 2380 ttt aac tgg ggt cct tgg gat ggc ggc atg gtt aac cca gcg ctt aaa 7200 Phe Asn Trp Gly Pro Trp Asp Gly Gly Met Val Asn Pro Ala Leu Lys 2385 2390 2395 2400 aag atg ttt acc gag cgt ggt gtg tac gtt att cca cta aaa gca ggt 7248 Lys Thr Glu Arg Gly Val Tyr Val Ile Pro Leu Lys Ala Gly 2405 2410 2415 gca gag cta ttt gcc act cag cta ttg gct gaa act ggc gtg cag ttg 7296 Ala Glu Leu Phe Ala Thr Gln Leu Leu Ala Glu Thr Gly Val Gln Leu 2420 2425 2430 ctc att ggt acg tca atg caa ggt ggc agc gac act aaa gca act gag 7344 Leu Ile Gly Thr Ser Met Gln Gly Gly Ser Asp Thr Lys Ala Thr Glu 2435 2440 2445 act gct tct gta aaa aag ctt aat gcg ggt gag gtg cta agt gca tcg 7392 Thr Ala Ser Val Lys Lys Leu Asn Ala Gly Glu Val Leu Ser Ala Ser 2450 2455 2460 cat ccg cgt gct ggt gca caa aaa aca cca cta caa gct gtc act gca 7440 His Pro Arg Ala Gly Ala Gln Lys Thr Pro Leu Gln Ala Val Thr Ala 2465 2470 2475 2480 acg cgt ctg tta acc cca agt gcc atg gtc ttc att gaa gat cac cgc 7488 Thr Arg Leu Leu Thr Pro Ser Ala Met Val Phe Ile Glu Asp His A rg 2485 2490 2495 att ggc ggt aac agt gtg ttg cca acg gta tgc gcc atc gac tgg atg 7536 Ile Gly Gly Asn Ser Val Leu Pro Thr Val Cys Ala Ile Asp Trp Met 2500 2505 2510 cgt gaa gcg gca agc gc atg ctt caa gtt aag gta ctt gat 7584 Arg Glu Ala Ala Ser Asp Met Leu Gly Ala Gln Val Lys Val Leu Asp 2515 2520 2525 tac aag cta tta aaa ggc att gta ttt gag act gat gag ccg caa gag 7632 Tyr Lys Leu Leu Lys Gly Val Phe Glu Thr Asp Glu Pro Gln Glu 2530 2535 2540 tta aca ctt gag cta acg cca gac gat tca gac gaa gct acg cta caa 7680 Leu Thr Leu Glu Leu Thr Pro Asp Asp Ser Asp Glu Ala Thr Leu Gln 2545 2550 2555 2560 gca tta atc agc tgt aat ggg cgt ccg caa tac aag gcg acg ctt atc 7728 Ala Leu Ile Ser Cys Asn Gly Arg Pro Gln Tyr Lys Ala Thr Leu Ile 2565 2570 2575 agt gat aat gcc gat att aag caa ctt aac aag cag ttt gat agc 7776 Ser Asp Asn Ala Asp Ile Lys Gln Leu Asn Lys Gln Phe Asp Leu Ser 2580 2585 2590 gct aag gcg att acc aca gca aaa gag ctt tat agc aac ggc acc ttg 7824 Ala Lys Ala Ile Thr Thr Ala Lys Glu Leu Tyr Ser Asn Gly Thr Leu 2595 2600 2605 ttc cac ggt ccg cgt cta caa ggg atc caa tct gta gtg cag ttc gat 7872 Phe His Gly Pro Arg Leu Gln Gly Ile Gln Ser Val Val Gln Phe Asp 2610 2615 2620 gat caa ggc tta att gct aaa gtc gct ctg cct aag gtt gaa ctt agc 7920 Asp Gln Gly Leu Ile Ala Lys Val Ala Leu Pro Lys Val Glu Leu Ser 2625 2630 2635 2640 gat tgt ggt gag ttc ttg ccg caa acc cac agt caa cct 7968 Asp Cys Gly Glu Plu Leu Pro Gln Thr His Met Gly Gly Ser Gln Pro 2645 2650 2655 ttt gct gag gac ttg cta tta caa gct atg ctg gtt tgg gct cgc ctt 8016 Phe Ala Glu Asp Leu Leu Leu Gln Ala Mla Leu Val Trp Ala Arg Leu 2660 2665 2670 aaa act ggc tcg gca agt ttg cca tca agc att ggt gag ttt acc tca 8064 Lys Thr Gly Ser Ala Ser Leu Pro Ser Ser Ile Gly Glu Phe Thr Ser 2675 2680 2685 tac caa cca atg gcc ttt ggt gaa act ggt acc ata gag ctt gaa gtg 8112 Tyr Gln Pro Met Ala Phe Gly Glu Thr Gly Thr Ile Glu Leu Glu Val 2690 2695 2700 att aag cac aac aaa cgc tca ctt gaa gcg aat gtt gcg cta tat cgt 8160 Ile Lys His Asn Lys Arg Ser Leu Glu Ala Asn Val Ala Leu Tyr Arg 2705 2710 2715 2720 gac aac ggc gag tta agt gcc atg ttt aag tca gct aaa atc acc att 8208 Asp Asn Gly Glu Leu Ser Ala Met Phe Lys Ser Ala Lys Ile Thr Ile 2725 2730 2735 agc aaa agc tta aat tca gca ttt tta cct gct gtc tta gca aac gac 8256 Ser Lys Ser Leu Asn Ser Ala Phe Leu Pro Ala Val Leu Ala Asn Asp 2740 2745 2750 agt gag gg aat 8268 Ser Glu Ala Asn 2755 <210> 7 <211> 2756 <212> PRT <400> 1 Met Ser Gln Thr Ser Lys Pro Thr Asn Ser Ala Thr Glu Gln Ala Gln 1 5 10 15 Asp Ser Gln Ala Asp Ser Arg Leu Asn Lys Arg Leu Lys Asp Met Pro 20 25 30 Ile Ala Ile Val Gly Met Ala Ser Ile Phe Ala Asn Ser Arg Tyr Leu 35 40 45 Asn Lys Phe Trp Asp Leu Ile Ser Glu Lys Ile Asp Ala Ile Thr Glu 50 55 60 Leu Pro Ser Thr His Trp Gln Pro Glu Glu Tyr Tyr Asp Ala Asp Lys 65 70 75 80 Thr Ala Ala Asp Lys Ser Tyr Cys Lys Arg Gly Gly Phe Leu Pro Asp 85 90 95 Val Asp Phe Asn Pro Met Glu Phe Gly Leu Pro Pro Asn Ile Leu Glu 100 105 110 Leu Thr Asp Ser Ser Gln Leu Leu Ser Leu Ile Val Ala Lys Glu Val 115 120 125 Leu Ala Asp Ala Asn Leu Pro Glu Asn Tyr Asp Arg Asp Lys Ile Gly 130 135 140 Ile Thr Leu Gly Val Gly Gly Gly Gln Lys Ile Ser His Ser Leu Thr 145 150 155 160 Ala Arg Leu Gln Tyr Pro Val Leu Lys Lys Val Phe Ala Asn Ser Gly 165 170 175 Ile Ser Asp Thr Asp Ser Glu Met Leu Ile Lys Lys Phe Gln Asp Gln 180 185 190 Tyr Val His Trp Glu Glu Asn Ser Phe Pro Gly Ser Leu Gly Asn Val 195 200 205 Ile Ala Gly Ar g Ile Ala Asn Arg Phe Asp Phe Gly Gly Met Asn Cys 210 215 220 Val Val Asp Ala Ala Cys Ala Gly Ser Leu Ala Ala Met Arg Met Ala 225 230 235 240 Leu Thr Glu Leu Thr Glu Gly Arg Ser Glu Met Met Ile Thr Gly Gly 245 250 255 Val Cys Thr Asp Asn Ser Pro Ser Met Tyr Met Ser Phe Ser Lys Thr 260 265 270 Pro Ala Phe Thr Thr Asn Glu Thr Ile Gln Pro Phe Asp Ile Asp Ser 275 280 285 Lys Gly Met Met Ile Gly Glu Gly Ile Gly Met Val Ala Leu Lys Arg 290 295 300 Leu Glu Asp Ala Glu Arg Asp Gly Asp Arg Ile Tyr Ser Val Ile Lys 305 310 315 320 Gly Val Gly Ala Ser Ser Asp Gly Lys Phe Lys Ser Ile Tyr Ala Pro 325 330 335 Arg Pro Ser Gly Gln Ala Lys Ala Leu Asn Arg Ala Tyr Asp Asp Ala 340 345 350 Gly Phe Ala Pro His Thr Leu Gly Leu Ile Glu Ala His Gly Thr Gly 355 360 365 Thr Ala Ala Gly Asp Ala Ala Glu Phe Ala Gly Leu Cys Ser Val Phe 370 375 380 Ala Glu Gly Asn Asp Thr Lys Gln His Ile Ala Leu Gly Ser Val Lys 385 390 395 400 Ser Gln Ile Gly His Thr Lys Ser Thr Ala Gly Thr Ala Gly Leu Ile 405 410 415 Lys Ala Ala L eu Ala Leu His His Lys Val Leu Pro Pro Thr Ile Asn 420 425 430 Val Ser Gln Pro Ser Pro Lys Leu Asp Ile Glu Asn Ser Pro Phe Tyr 435 440 445 Leu Asn Thr Glu Thr Arg Pro Trp Leu Pro Arg Val Asp Gly Thr Pro 450 455 460 Arg Arg Ala Gly Ile Ser Ser Phe Gly Phe Gly Gly Thr Asn Phe His 465 470 475 480 Phe Val Leu Glu Glu Tyr Asn Gln Glu His Ser Arg Thr Asp Ser Glu 485 490 495 Lys Ala Lys Tyr Arg Gln Arg Gln Val Ala Gln Ser Phe Leu Val Ser 500 505 510 Ala Ser Asp Lys Ala Ser Leu Ile Asn Glu Leu Asn Val Leu Ala Ala 515 520 525 Ser Ala Ser Gln Ala Glu Phe Ile Leu Lys Asp Ala Ala Ala Asn Tyr 530 535 540 Gly Val Arg Glu Leu Asp Lys Asn Ala Pro Arg Ile Gly Leu Val Ala 545 550 555 560 Asn Thr Ala Glu Glu Leu Ala Gly Leu Ile Lys Gln Ala Leu Ala Lys 565 570 575 Leu Ala Ala Ser Asp Asp Asn Ala Trp Gln Leu Pro Gly Gly Thr Ser 580 585 590 Tyr Arg Ala Ala Ala Val Glu Gly Lys Val Ala Ala Leu Phe Ala Gly 595 600 605 Gln Gly Ser Gln Tyr Leu Asn Met Gly Arg Asp Leu Thr Cys Tyr Tyr 610 615 620 620 Pro Glu Met Arg G ln Gln Phe Val Thr Ala Asp Lys Val Phe Ala Ala 625 630 635 640 Asn Asp Lys Thr Pro Leu Ser Gln Thr Leu Tyr Pro Lys Pro Val Phe 645 650 655 Asn Lys Asp Glu Leu Lys Ala Gln Glu Ala Ile Leu Thr Asn Thr Ala 660 665 670 Asn Ala Gln Ser Ala Ile Gly Ala Ile Ser Met Gly Gln Tyr Asp Leu 675 680 685 Phe Thr Ala Ala Gly Phe Asn Ala Asp Met Val Ala Gly His Ser Phe 690 695 700 Gly Glu Leu Ser Ala Leu Cys Ala Ala Gly Val Ile Ser Ala Asp Asp 705 710 715 715 720 Tyr Tyr Lys Leu Ala Phe Ala Arg Gly Glu Alu Met Ala Thr Lys Ala 725 730 735 Pro Ala Lys Asp Gly Val Glu Ala Asp Ala Gly Ala Met Phe Ala Ile 740 745 750 Ile Thr Lys Ser Ala Ala Asp Leu Glu Thr Val Glu Ala Thr Ile Ala 755 760 765 Lys Phe Asp Gly Val Lys Val Ala Asn Tyr Asn Ala Pro Thr Gln Ser 770 775 780 Val Ile Ala Gly Pro Thr Ala Thr Thr Ala Asp Ala Ala Lys Ala Leu 785 790 795 800 Thr Glu Leu Gly Tyr Lys Ala Ile Asn Leu Pro Val Ser Gly Ala Phe 805 810 815 His Thr Glu Leu Val Gly His Ala Gln Ala Pro Phe Ala Lys Ala Ile 820 825 830 Asp Ala Ala LysPhe Thr Lys Thr Ser Arg Ala Leu Tyr Ser Asn Ala 835 840 845 Thr Gly Gly Leu Tyr Glu Ser Thr Ala Ala Lys Ile Lys Ala Ser Phe 850 855 860 Lys Lys His Met Leu Gln Ser Val Arg Phe Thr Ser Gln Leu Glu Ala 865 870 875 880 Met Tyr Asn Asp Gly Ala Arg Val Phe Val Glu Phe Gly Pro Lys Asn 885 890 895 Ile Leu Gln Lys Leu Val Gln Gly Thr Leu Val Asn Thr Glu Asn Glu 900 905 910 Val Cys Thr Ile Ser Ile Asn Pro Asn Pro Lys Val Asp Ser Asp Leu 915 920 925 925 Gln Leu Lys Gln Ala Ala Met Gln Leu Ala Val Thr Gly Val Val Leu 930 935 940 Ser Glu Ile Asp Pro Tyr Gln Ala Asp Ile Ala Ala Pro Ala Lys Lys 945 950 955 960 Ser Pro Met Ser Ile Ser Leu Asn Ala Ala Asn His Ile Ser Lys Ala 965 970 975 Thr Arg Ala Lys Met Ala Lys Ser Leu Glu Thr Gly Ile Val Thr Ser 980 985 990 Gln Ile Glu His Val Ile Glu Glu Lys Ile Val Glu Val Glu Lys Leu 995 1000 1005 Val Glu Val Glu Lys Ile Val Glu Lys Val Val Glu Val Glu Lys Val 1010 1015 1020 Val Glu Val Glu Ala Pro Val Asn Ser Val Gln Ala Asn Ala Ile Gln 1025 1030 1035 1040 Thr Arg Ser Val Val Ala Pro Val Ile Glu Asn Gln Val Val Ser Lys 1045 1050 1055 Asn Ser Lys Pro Ala Val Gln Ser Ile Ser Gly Asp Ala Leu Ser Asn 1060 1065 1070 Phe Phe Ala Ala Gln Gln Gln Thr Ala Gln Leu His Gln Gln Phe Leu 1075 1080 1085 Ala Ile Pro Gln Gln Tyr Gly Glu Thr Phe Thr Thr Leu Met Thr Glu 1090 1095 1100 Gln Ala Lys Leu Ala Ser Ser Gly Val Ala Ile Pro Glu Ser Leu Gln 1105 1110 1115 1120 Arg Ser Met Glu Gln Phe His Gln Leu Gln Ala Gln Thr Leu Gln Ser 1125 1130 1135 His Thr Gln Phe Leu Glu Met Gln Ala Gly Ser Asn Ile Ala Ala Leu 1140 1145 1150 Asn Leu Leu Asn Ser Ser Gln Ala Thr Tyr Ala Pro Ala Ile His Asn 1155 1160 1165 Glu Ala Ile Gln Ser Gln Val Val Gln Ser Gln Thr Ala Val Gln Pro 1170 1175 1180 Val Ile Ser Thr Gln Val Asn His Val Ser Glu Gln Pro Thr Gln Ala 1185 1190 1195 1200 Pro Ala Pro Lys Ala Gln Pro Ala Pro Val Thr Thr Pro Val Gln Thr 1205 1210 1215 Ala Pro Ala Gln Val Val Arg Gln Ala Ala Pro Val Gln Ala Ala Ile 1220 1225 1230 Glu Pro Ile Asn Thr Ser Val Ala Thr Thr Thr Pro Ser Ala Phe Ser 1235 1240 1245 Ala Glu Thr Ala Leu Ser Ala Thr Lys Val Gln Ala Thr Met Leu Glu 1250 1255 1260 Val Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu Met Leu Glu Leu Glu 1265 1270 1275 1280 Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu 1285 1290 1295 Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly Leu Pro Glu Leu Ser 1300 1305 1310 Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu Ile Val Asp Tyr 1315 1320 1325 Met Gly Ser Lys Leu Pro Ala Glu Gly Ser Met Asn Ser Gln Leu Ser 1330 1335 1340 Thr Gly Ser Ala Ala Ala Thr Pro Ala Ala Asn Gly Leu Ser Ala Glu 1345 1350 1355 1360 Lys Val Gln Ala Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr 1365 1370 1375 Pro Thr Glu Met Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly 1380 1385 1390 Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu 1395 1400 1405 Leu Pro Gly Leu Pro Glu Leu Ser Pro Glu Asp Leu Ala Glu Cys Arg 1410 1415 1420 Thr Leu Gly Glu Ile Val Asp Tyr Met Asn Ser Lys Leu Ala Asp Gly 1425 1430 1435 1440 Ser Ly s Leu Pro Ala Glu Gly Ser Met Asn Ser Gln Leu Ser Thr Ser 1445 1450 1455 Ala Ala Ala Ala Thr Pro Ala Ala Asn Gly Leu Ser Ala Glu Lys Val 1460 1465 1470 Gln Ala Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr Pro Thr 1475 1480 1485 Glu Met Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly Ile Asp 1490 1495 1500 Ser Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro 1505 1510 1515 1520 Gly Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu 1525 1530 1535 Gly Glu Ile Val Thr Tyr Met Asn Ser Lys Leu Ala Asp Gly Ser Lys 1540 1545 1550 Leu Pro Ala Glu Gly Ser Met His Tyr Gln Leu Ser Thr Ser Thr Ala 1555 1560 1565 Ala Ala Thr Pro Val Ala Asn Gly Leu Ser Ala Glu Lys Val Gln Ala 1570 1575 1580 Thr Met Met Ser Val Val Ala Asp Lys Thr Gly Tyr Pro Thr Glu Met 1585 1590 1595 1600 Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile 1605 1610 1615 Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly Leu 1620 1625 1630 Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu 1635 1640 1645 Ile Val Asp Tyr Met Gly Ser Lys Leu Pro Ala Glu Gly Ser Ala Asn 1650 1655 1660 Thr Ser Ala Ala Ala Ser Leu Asn Val Ser Ala Val Ala Ala Pro Gln 1665 1670 1675 1680 Ala Ala Ala Ala Thr Pro Val Ser Asn Gly Leu Ser Ala Glu Lys Val Gln 1685 1690 1695 Ser Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu 1700 1705 1710 Met Leu Glu Leu Gly Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser 1715 1720 1725 Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly 1730 1735 1740 Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly 1745 1750 1755 1760 Glu Ile Val Asp Tyr Met Asn Ser Lys Leu Ala Asp Gly Ser Lys Leu 1765 1770 1775 Pro Ala Glu Gly Ser Ala Asn Thr Ser Ala Thr Ala Ala Thr Pro Ala 1780 1785 1790 Val Asn Gly Leu Ser Ala Asp Lys Val Gln Ala Thr Met Met Ser Val 1795 1800 1805 Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu Met Leu Glu Leu Gly Met 1810 1815 1820 Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile 1825 1830 1835 1840 Leu G ly Thr Val Gln Asp Glu Leu Pro Gly Leu Pro Glu Leu Asn Pro 1845 1850 1855 Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu Ile Val Ser Tyr Met 1860 1865 1870 Asn Ser Gln Leu Ala Asp Gly Ser Lys Leu Ser Thr Ser Ala Ala Glu 1875 1880 1885 Gly Ser Ala Asp Thr Ser Ala Ala Asn Ala Ala Lys Pro Ala Ala Ile 1890 1895 1900 Ser Ala Glu Pro Ser Val Glu Leu Pro Pro His Ser Glu Val Ala Leu 1905 1910 1915 1920 Lys Lys Leu Asn Ala Ala Asn Lys Leu Glu Asn Cys Phe Ala Ala Asp 1925 1930 1935 Ala Ser Val Val Ile Asn Asp Asp Gly His Asn Ala Gly Val Leu Ala 1940 1945 1950 Glu Lys Leu Ile Lys Gln Gly Leu Lys Val Ala Val Val Arg Leu Pro 1955 1960 1965 Lys Gly Gln Pro Gln Ser Pro Leu Ser Ser Asp Val Ala Ser Phe Glu 1970 1975 1980 Leu Ala Ser Ser Gln Glu Ser Glu Leu Glu Ala Ser Ile Thr Ala Val 1985 1990 1995 2000 Ile Ala Gla Iln Glu Thr Gln Val Gly Ala Ile Gly Gly Phe Ile His 2005 2010 2015 Leu Gln Pro Glu Ala Asn Thr Glu Glu Gln Thr Ala Val Asn Leu Asp 2020 2025 2030 Ala Gln Ser Phe Thr His Val Ser Asn Ala Phe Le u Trp Ala Lys Leu 2035 2040 2045 Leu Gln Pro Lys Leu Val Ala Gly Ala Asp Ala Arg Arg Cys Phe Val 2050 2055 2060 Thr Val Ser Arg Ile Asp Gly Gly Phe Gly Tyr Leu Asn Thrh Asp Ala 2065 2070 2075 2080 Leu Lys Asp Ala Glu Leu Asn Gln Ala Ala Leu Ala Gly Leu Thr Lys 2085 2090 2095 Thr Leu Ser His Glu Trp Pro Gln Val Phe Cys Arg Ala Leu Asp Ile 2100 2105 2110 Ala Thr Asp Val Asp Ala Thr His Leu Ala Asp Ala Ile Thr Ser Glu 2115 2120 2125 Leu Phe Asp Ser Gln Ala Gln Leu Pro Glu Val Gly Leu Ser Leu Ile 2130 2135 2140 Asp Gly Lys Val Asn Arg Val Thr Leu Val Ala Ala Glu Ala Ala Asp 2145 2150 2155 2160 Lys Thr Ala Lys Ala Glu Leu Asn Ser Thr Asp Lys Ile Leu Val Thr 2165 2170 2175 Gly Gly Ala Lys Gly Val Thr Phe Glu Cys Ala Leu Ala Leu Ala Ser 2180 2185 2190 Arg Ser Gln Ser His Phe Ile Leu Ala Gly Arg Ser Glu Leu Gln Ala 2195 2200 2205 Leu Pro Ser Trp Ala Glu Gly Lys Gln Thr Ser Glu Leu Lys Ser Ala 2210 2215 2220 Ala Ile Ala His Ile Ile Ser Thr Gly Gln Lys Pro Thr Pro Lys Gln 2225 2230 2235 2240 Val Glu Ala Ala Val Trp Pro Val Gln Ser Ser Ile Glu Ile Asn Ala 2245 2250 2255 Ala Leu Ala Ala Phe Asn Lys Val Gly Ala Ser Ala Glu Tyr Val Ser 2260 2265 2270 Met Asp Val Thr Asp Ser Ala Ala Ile Thr Ala Ala Leu Asn Gly Arg 2275 2280 2285 Ser Asn Glu Ile Thr Gly Leu Ile His Gly Ala Gly Val Leu Ala Asp 2290 2295 2300 Lys His Ile Gln Asp Lys Thr Leu Ala Glu Leu Ala Lys Val Tyr Gly 2305 2310 2315 2320 Thr Lys Val Asn Gly Leu Lys Ala Leu Leu Ala Ala Leu Glu Pro Ser 2325 2330 2335 Lys Ile Lys Leu Leu Ala Met Phe Ser Ser Ala Ala Gly Phe Tyr Gly 2340 2345 2350 Asn Ile Gly Gln Ser Asp Tyr Ala Met Ser Asn Asp Ile Leu Asn Lys 2355 2360 2365 Ala Ala Leu Gln Phe Thr Ala Arg Asn Pro Gln Ala Lys Val Met Ser 2370 2375 2380 Phe Asn Trp Gly Pro Trp Asp Gly Gly Met Val Asn Pro Ala Leu Lys 2385 2390 2395 2400 Lys Met Phe Thr Glu Arg Gly Val Tyr Val Ile Pro Leu Lys Ala Gly 2405 2410 2415 Ala Glu Leu Phe Ala Thr Gln Leu Leu Ala Glu Thr Gly Val Gln Leu 2420 2425 2430 Leu Ile Gly Thr Ser Met Gln Gly Gly Ser Asp T hr Lys Ala Thr Glu 2435 2440 2445 Thr Ala Ser Val Lys Lys Leu Asn Ala Gly Glu Val Leu Ser Ala Ser 2450 2455 2460 His Pro Arg Ala Gly Ala Gln Lys Thr Pro Leu Gln Ala Val Thr Ala 2465 2470 2475 2480 Thr Arg Leu Leu Thr Pro Ser Ala Met Val Phe Ile Glu Asp His Arg 2485 2490 2495 Ile Gly Gly Asn Ser Val Leu Pro Thr Val Cys Ala Ile Asp Trp Met 2500 2505 2510 Arg Glu Ala Ala Ser Asp Met Leu Gly Ala Gln Val Lys Val Leu Asp 2515 2520 2525 Tyr Lys Leu Leu Lys Gly Ile Val Phe Glu Thr Asp Glu Pro Gln Glu 2530 2535 2540 Leu Thr Leu Glu Leu Thr Pro Asp Asp Ser Asp Glu Ala Thr Leu Gln 2545 2550 2555 2560 Ala Leu Ile Ser Cys Asn Gly Arg Pro Gln Tyr Lys Ala Thr Leu Ile 2565 2570 2575 Ser Asp Asn Ala Asp Ile Lys Gln Leu Asn Lys Gln Phe Asp Leu Ser 2580 2585 2585 2590 Ala Lys Ala Ile Thr Thr Ala Lys Glu Leu Tyr Ser Asn Gly Thr Leu 2595 2600 2605 Phe His Gly Pro Arg Leu Gln Gly Ile Gln Ser Val Val Gln Phe Asp 2610 2615 2620 Asp Gln Gly Leu Ile Ala Lys Val Ala Leu Pro Lys Val Glu Leu Ser 2625 2630 2635 2640 Asp Cys Gly Glu Phe Leu Pro Gln Thr His Met Gly Gly Ser Gln Pro 2645 2650 2655 Phe Ala Glu Asp Leu Leu Leu Gln Ala Met Leu Val Trp Ala Arg Leu 2660 2665 2670 Lys Thr Gly Ser Ala Ser Leu Pro Ser Ser Ile Gly Glu Phe Thr Ser 2675 2680 2685 Tyr Gln Pro Met Ala Phe Gly Glu Thr Gly Thr Ile Glu Leu Glu Val 2690 2695 2700 Ile Lys His Asn Lys Arg Ser Leu Glu Ala Asn Val Ala Leu Tyr Arg 2705 2710 2715 2720 Asp Asn Gly Glu Leu Ser Ala Met Phe Lys Ser Ala Lys Ile Thr Ile 2725 2730 2735 Ser Lys Ser Leu Asn Ser Ala Phe Leu Pro Ala Val Leu Ala Asn Asp 2740 2745 2750 Ser Glu Ala Asn 2755 <210> 8 <211> 2340 <212> DNA <213> Shewanella putrefaciens SCRC-2874 (FERM BP-1625) <400> 1 gtg gaa caa acg cct aaa gct agt gcg atg ccg ctg cgc atc gca ctt 48 Val Glu Gln Thr Pro Lys Ala Ser Ala Met Pro Leu Arg Ile Ala Leu 1 5 10 15 atc tta ctg cca aca ccg cag ttt gaa gtt aac tct gtc gac cag tca 96 Ile Leu Leu Pro Thr Pro Gln Phe Glu Val Asn Ser Val Asp Gln Ser 20 25 30 gta tta gcc agc tat caa aca ctg cag cct gag cta aat gcc ctg ctt 144 Val Leu Ala Ser Tyr Gln Thr Leu Gln Pro Glu Leu Asn Ala Leu Leu 35 40 45 aat agt gcg ccg aca cct gaa atg ctc agc atc act atc tca gat gat 192 Asn Ser Ala Pro Thr Pro Glu Met Leu Ser Ile Thr Ile Ser Asp Asp 50 55 60 agc gat gca aac agc ttt gag tcg cag cta aat gct gcg acc aac gca 240 Ser Asp Ala Asn Ser Phe Glu Ser Gln Leu Asn Ala Ala Thr Asn Ala 65 70 75 80 att aac aat ggc tat atc gtc aag ctt gct acg gca act cac gct ttg 288 Ile Asn Asn Gly Tyr Ile Val Lys Leu Ala Thr Ala Thr His Ala Leu 85 90 95 tta atg ctg cct gca tta aaa gcg gcg caa atg cgg atc cat cct cat 336 Leu Met Leu Pro Ala Leu Lys Ala Ala Gln Met Arg Ile His Pro His 100 105 110 gcg cag ctt gcc gct atg cag caa gct aaa tcg acg cca atg agt caa 384 Ala Gln Leu Ala Ala Met Gln Gln Ala Lys Ser Thr Pro Met Ser Gln 115 120 125 gta tct ggt gag cta aag ctt ggc gct aat gcg cta agc cta gct cag 432 Val Ser Gly Glu Leu Lys Leu Gly Ala Asn Ala Leu Ser Leu Ala Gln 130 135 140 act aat gcg ctg tct cat gct tta agc caa gcc aag cgt aac tta act 480 Thr Asn Ala Leu Ser His Ala Leu Ser Gln Ala Lys Arg Asn Leu Thr 145 150 155 160 gat gtc agc gtg aat gag tgt ttt gag aac ctc aaa agt gaa cag cag 528 Asp Val Ser Val Asn Glu Cys Phe Glu Asn Leu Lys Ser Glu Gln Gln 165 170 175 tttt aca gag gtt tat tcg ctt att cag caa ctt gct agc cgc acc cat 576 Phe Thr Glu Val Tyr Ser Leu Ile Gln Gln Leu Ala Ser Arg Thr His 180 185 190 gtg aga aaa gag gtt aat caa ggt gtg gaa ctt ggc cct aaa caa gcc 624 Val Arg Lys Glu Val Asn Gln Gly Val Glu Leu Gly Pro Lys Gln Ala 195 200 205 aaa agc cac tat tgg ttt agc gaa ttt cac caa aac cgt gtt gct gcc 672 Lys Ser His Tyr Trp Phe Ser Glu Phe His Gln Asn Arg Val Ala Ala 210 215 220 atc aac ttt att aat ggc caa caa gca acc agc tat gtg ctt act caa 720 Ile Asn Phe Ile Asn Gly Gln Gln Ala Thr Ser Tyr Val Leu Thr Gln 225 230 235 240 ggt tca gga ttg tta gct gcg aaa tca atg cta aac cag caa aga tta 768 Gly Ser Gly Leu Leu Ala Ala Lys Ser Met Leu Asn Gln Gln Arg Leu 245 250 255 atg ttt atc ttg ccg ggt aac agt cag caa caa ata acc gca tca ata 816 Met Phe Ile Leu Pro Gly Asn Ser Gln Gln Gln Ile Thr Ala Ser Ile 260 265 270 act cag tta atg cag caa tta gag cgt ttg cag gta act gag gtt aat 864 Thr Gln Leu Met Gln Gln Leu Glu Arg Leu Gln Val Thr Glu Val Asn 275 280 285 gag ctt tct cta gaa tgc caa cta gag ctg ctc agc ata atg tat gac 912 Glu Leu Ser Leu Glu Cys Gln Leu Glu Leu Leu Ser Ile Met Tyr Asp 290 295 300 aac tta gtc aac gca gac aaa ctc gat agt g 960 Asn Leu Val Asn Ala Asp Lys Leu Thr Thr Arg Asp Ser Lys Pro Ala 305 310 315 320 tat cag gct gtg att caa gca agc tct gtt agc gct gca aag caa gag 1008 Tyr Gln Ala Val Ile Gln Ala Ser Ser Val Ser Al a Ala Lys Gln Glu 325 330 335 tta agc gcg ctt aac gat gca ctc aca gcg ctg ttt gct gag caa aca 1056 Leu Ser Ala Leu Asn Asp Ala Leu Thr Ala Leu Phe Ala Glu Gln Thr 340 345 350 aac gcc aca tca ac aaa ggc tta atc caa tac aaa aca ccg gcg 1104 Asn Ala Thr Ser Thr Asn Lys Gly Leu Ile Gln Tyr Lys Thr Pro Ala 355 360 365 ggc agt tac tta acc cta aca ccg ctt ggc agc aac aat gac aac gcc 1152 Gly Seryr Leu Thr Leu Thr Pro Leu Gly Ser Asn Asn Asp Asn Ala 370 375 380 caa gcg ggt ctt gct ttt gtc tat ccg ggt gtg gga acg gtt tac gcc 1200 Gln Ala Gly Leu Ala Phe Val Tyr Pro Gly Val Gly Thr Val Tyr Ala 385 390 395 400 gat atg ctt aat gag ctg cat cag tac ttc cct gcg ctt tac gcc aaa 1248 Asp Met Leu Asn Glu Leu His Gln Tyr Phe Pro Ala Leu Tyr Ala Lys 405 410 415 ctt gag cgt gaa ggc gat tta agag gat caa gca gaa gat atc 1296 Leu Glu Arg Glu Gly Asp Leu Lys Ala Met Leu Gln Ala Glu Asp Ile 420 425 430 tat cat ctt gac cct aaa cat gct gcc caa atg agc tta ggt gac tta 1344 Tyr His Leu Asp Pro Lys His Ala Ala Gln Met Ser Leu Gly Asp Leu 435 440 445 gcc att gct ggc gtg ggg agc agc tac ctg tta act cag ctg ctc acc 1392 Ala Ile Ala Gly Val Gly Ser Ser Tyr Leu Leu Thr Gln Leu Leu Thr 450 455 460 gat g ttt aat att aag cct aat ttt gca tta ggt tac tca atg ggt 1440 Asp Glu Phe Asn Ile Lys Pro Asn Phe Ala Leu Gly Tyr Ser Met Gly 465 470 475 475 480 gaa gca tca atg tgg gca agc tta ggc gta tgg caaac gcg 1488 Glu Ala Ser Met Trp Ala Ser Leu Gly Val Trp Gln Asn Pro His Ala 485 490 495 ctg atc agc aaa acc caa acc gac ccg cta ttt act tct gct att tcc 1536 Leu Ile Ser Lys Thr Gln Thr Asp Pro Leu Phe Thr Ser Ala Ile Ser 500 505 510 ggc aaa ttg acc gcg gtt aga caa gct tgg cag ctt gat gat acc gca 1584 Gly Lys Leu Thr Ala Val Arg Gln Ala Trp Gln Leu Asp Asp Thr Ala 515 520 525 gcg gaa atc cag tgg aatg ttt gtg gtt aga agt gaa gca gcg ccg 1632 Ala Glu Ile Gln Trp Asn Ser Phe Val Val Arg Ser Glu Ala Ala Pro 530 535 540 att gaa gcc ttg cta aaa gat tac cca cac gct tac ctc gcg att att 1680 Ile Ala Leu Leu Lys Asp Tyr Pro His Ala Tyr Leu Ala Ile Ile 545 550 555 560 caa ggg gat acc tgc gta atc gct ggc tgt gaa atc caa tgt aaa gcg 1728 Gln Gly Asp Thr Cys Val Ile Ala Gly Cys Glu Ile Gln Cy Ala 565 570 575 cta ctt gca gca ctg ggt aaa cgc ggt att gca gct aat cgt gta acg 1776 Leu Leu Ala Ala Leu Gly Lys Arg Gly Ile Ala Ala Asn Arg Val Thr 580 585 590 gcg atg cat acg cag cct gcg atg cat caa aat gtg atg gat 1824 Ala Met His Thr Gln Pro Ala Met Gln Glu His Gln Asn Val Met Asp 595 600 605 ttt tat ctg caa ccg tta aaa gca gag ctt cct agt gaa ata agc ttt 1872 Phe Tyr Leu Gln Pro Leu Lys Ala Glu Leu Pro Ser Glu Ile Ser Phe 610 615 620 atc agc gcc gct gat tta act gcc aag caa acg gtg agt gag caa gca 1920 Ile Ser Ala Ala Ala Asp Leu Thr Ala Lys Gln Thr Val Ser Glu Gln Ala 625 630 635 635 640 ctt agc agc caa gtc gtt gct cag tct att gcc gac acc ttc tgc caa 1968 Leu Ser Ser Gln Val Val Ala Gln Ser Ile Ala Asp Thr Phe Cys Gln 645 650 655 acc ttg gac ttt acc gcg cta gta cat cac gcc caa cat c aa ggc gct 2016 Thr Leu Asp Phe Thr Ala Leu Val His His Ala Gln His Gln Gly Ala 660 665 670 aag ctg ttt gtt gaa att ggc gcg gat aga caa aac tgc acc ttg ata 2064 Lys Leu Phe Val Glu Ile Gly Ala Asg Arg Gln Asn Cys Thr Leu Ile 675 680 685 gac aag att gtt aaa caa gat ggt gcc agc agt gta caa cat caa cct 2112 Asp Lys Ile Val Lys Gln Asp Gly Ala Ser Ser Val Gln His Gln Pro 690 695 700 tgt tgc aca gtg cct atg aac gca aaa ggt agc caa gat att acc agc 2160 Cys Cys Thr Val Pro Met Asn Ala Lys Gly Ser Gln Asp Ile Thr Ser 705 710 715 720 gtg att aaa gcg ctt ggc caa tta att agc cat cag gtg cca tta tcg 2 al Ile Lys Ala Leu Gly Gln Leu Ile Ser His Gln Val Pro Leu Ser 725 730 735 gtg caa cca ttt att gat gga ctc aag cgc gag cta aca ctt tgc caa 2256 Val Gln Pro Phe Ile Asp Gly Leu Lys Arg Glu Leu Thr Leu Cys Gln 740 745 750 ttg acc agc caa cag ctg gca gca cat gca aat gtt gac agc aag ttt 2304 Leu Thr Ser Gln Gln Leu Ala Ala His Ala Asn Val Asp Ser Lys Phe 755 760 765 gag tct aac caa gac cat tta ctt caa ggg gaa gtc 2340 Glu Ser Asn Gln Asp His Leu Leu Gln Gly Glu Val 770 775 780 <210> 9 <211> 780 <212> PRT <400> 1 Val Glu Gln Thr Pro Lys Ala Ser Ala Met Pro Leu Arg Ile Ala Leu 1 5 10 15 Ile Leu Leu Pro Thr Pro Gln Phe Glu Val Asn Ser Val Asp Gln Ser 20 25 30 Val Leu Ala Ser Tyr Gln Thr Leu Gln Pro Glu Leu Asn Ala Leu Leu 35 40 45 Asn Ser Ala Pro Thr Pro Glu Met Leu Ser Ile Thr Ile Ser Asp Asp 50 55 60 Ser Asp Ala Asn Ser Phe Glu Ser Gln Leu Asn Ala Ala Thr Asn Ala 65 70 75 80 Ile Asn Asn Gly Tyr Ile Val Lys Leu Ala Thr Ala Thr His Ala Leu 85 90 95 Leu Met Leu Pro Ala Leu Lys Ala Ala Gln Met Arg Ile His Pro His 100 105 110 Ala Gln Leu Ala Ala Met Gln Gln Ala Lys Ser Thr Pro Met Ser Gln 115 120 125 Val Ser Gly Glu Leu Lys Leu Gly Ala Asn Ala Leu Ser Leu Ala Gln 130 135 140 Thr Asn Ala Leu Ser His Ala Leu Ser Gln Ala Lys Arg Asn Leu Thr 145 150 155 160 Asp Val Ser Val Asn Glu Cys Phe Glu Asn Leu Lys Ser Glu Gln Gln 165 170 175 Phe Thr Glu Val Tyr Ser Leu Ile Gln Gln Leu Ala Ser Arg Thr His 180 185 190 Val Arg Lys Glu Val Asn Gln Gly Val Glu Leu Gly Pro Lys Gln Ala 195 200 205 Lys Ser His Ty r Trp Phe Ser Glu Phe His Gln Asn Arg Val Ala Ala 210 215 220 Ile Asn Phe Ile Asn Gly Gln Gln Ala Thr Ser Tyr Val Leu Thr Gln 225 230 235 240 Gly Ser Gly Leu Leu Ala Ala Lys Ser Met Leu Asn Gln Gln Arg Leu 245 250 255 Met Phe Ile Leu Pro Gly Asn Ser Gln Gln Gln Ile Thr Ala Ser Ile 260 265 270 Thr Gln Leu Met Gln Gln Leu Glu Arg Leu Gln Val Thr Glu Val Asn 275 280 280 285 Glu Leu Ser Leu Glu Cys Gln Leu Glu Leu Leu Ser Ile Met Tyr Asp 290 295 300 Asn Leu Val Asn Ala Asp Lys Leu Thr Thr Arg Asp Ser Lys Pro Ala 305 310 315 320 Tyr Gln Ala Val Ile Gln Ala Ser Ser Val Ser Ala Ala Lys Gln Glu 325 330 335 Leu Ser Ala Leu Asn Asp Ala Leu Thr Ala Leu Phe Ala Glu Gln Thr 340 345 350 Asn Ala Thr Ser Thr Asn Lys Gly Leu Ile Gln Tyr Lys Thr Pro Ala 355 360 365 Gly Ser Tyr Leu Thr Leu Thr Pro Leu Gly Ser Asn Asn Asp Asn Ala 370 375 380 Gln Ala Gly Leu Ala Phe Val Tyr Pro Gly Val Gly Thr Val Tyr Ala 385 390 395 400 Asp Met Leu Asn Glu Leu His Gln Tyr Phe Pro Ala Leu Tyr Ala Lys 405 410 415 Leu Glu Arg G lu Gly Asp Leu Lys Ala Met Leu Gln Ala Glu Asp Ile 420 425 430 Tyr His Leu Asp Pro Lys His Ala Ala Gln Met Ser Leu Gly Asp Leu 435 440 445 Ala Ile Ala Gly Val Gly Ser Ser Tyr Leu Leu Thr Gln Leu Leu Thr 450 455 460 Asp Glu Phe Asn Ile Lys Pro Asn Phe Ala Leu Gly Tyr Ser Met Gly 465 470 475 480 Glu Ala Ser Met Trp Ala Ser Leu Gly Val Trp Gln Asn Pro His Ala 485 490 490 495 Leu Ile Ser Lys Thr Gln Thr Asp Pro Leu Phe Thr Ser Ala Ile Ser 500 505 510 510 Gly Lys Leu Thr Ala Val Arg Gln Ala Trp Gln Leu Asp Asp Thr Ala 515 520 525 Ala Glu Ile Gln Trp Asn Ser Phe Val Val Arg Ser Glu Ala Ala Pro 530 535 540 Ile Glu Ala Leu Leu Lys Asp Tyr Pro His Ala Tyr Leu Ala Ile Ile 545 550 555 560 Gln Gly Asp Thr Cys Val Ile Ala Gly Cys Glu Ile Gln Cys Lys Ala 565 570 575 Leu Leu Ala Ala Leu Gly Lys Arg Gly Ile Ala Ala Asn Arg Val Thr 580 585 590 Ala Met His Thr Gln Pro Ala Met Gln Glu His Gln Asn Val Met Asp 595 600 605 Phe Tyr Leu Gln Pro Leu Lys Ala Glu Leu Pro Ser Glu Ile Ser Phe 610 615 620 Ile Ser Ala Ala A sp Leu Thr Ala Lys Gln Thr Val Ser Glu Gln Ala 625 630 635 640 Leu Ser Ser Gln Val Val Ala Gln Ser Ile Ala Asp Thr Phe Cys Gln 645 650 655 Thr Leu Asp Phe Thr Ala Leu Val His His Ala Gln His Gln Gly Ala 660 665 670 Lys Leu Phe Val Glu Ile Gly Ala Asp Arg Gln Asn Cys Thr Leu Ile 675 680 685 Asp Lys Ile Val Lys Gln Asp Gly Ala Ser Ser Val Gln His Gln Pro 690 695 700 Cys Cys Thr Val Pro Met Asn Ala Lys Gly Ser Gln Asp Ile Thr Ser 705 710 715 715 720 Val Ile Lys Ala Leu Gly Gln Leu Ile Ser His Gln Val Pro Leu Ser 725 730 735 Val Gln Pro Phe Ile Asp Gly Leu Lys Arg Glu Leu Thr Leu Cys Gln 740 745 750 Leu Thr Ser Gln Gln Leu Ala Ala His Ala Asn Val Asp Ser Lys Phe 755 760 765 Glu Ser Asn Gln Asp His Leu Leu Gln Gly Glu Val 770 775 780 <210> 10 <211> 6015 <212> DNA <213> Shewanella putrefaciens SCRC-2874 (FERM BP-1625) <400> 1 atg tca tta cca gac aat gct tct aac cac ctt tct gcc aac cag aaa 48 Met Ser Leu Pro Asp Asn Ala Ser Asn His Leu Ser Ala Asn Gln Lys 1 5 10 15 ggc gca tct cag gca agt aaa acc agt aag caa agc aaa atc gcc att 96 Gly Ala Ser Gln Ala Ser Lys Thr Ser Lys Gln Ser Lys Ile Ala Ile 20 25 30 gtc ggt tta gcc act ctg tat cca gac gct aaa acc ccg caa gaa ttt 144 Val Gly Leu Ala Thr Leu Tyr Pro Asp Ala Lys Thr Pro Gln Glu Phe 35 40 45 tgg cag aat ttg ctg gat aaa cgc gac tct cgc agc acc tta act aac 192 Trp Gln Asn Leu Leu Asp Lys Arg Asp Ser Arg Ser Thr Leu Thr Asn 50 55 60 gaa aaa ctc ggc gct aac agc caa gat tat caa ggt gtg caa ggc caa 240 Glu Lys Leu Gly Ala Asn Ser Gln Asp Tyr Gln Gly Val Gln Gly Gln 65 70 75 80 tct gac cgt ttt tat tgt aat aaa ggc ggc tac attag ttc agc 288 Ser Asp Arg Phe Tyr Cys Asn Lys Gly Gly Tyr Ile Glu Asn Phe Ser 85 90 95 ttt aat gct gca ggc tac aaa ttg ccg gag caa agc tta aat ggc ttg 336 Phe Asn Ala Ala Gly Tyr Lysu Ser Leu Asn Gly Leu 100 105 110 gac gac agc ttc ctt tgg gcg ctc gat act agc cgt aac gca cta att 384 Asp Asp Ser Phe Leu Trp Ala Leu Asp Thr Ser Arg Asn Ala Leu Ile 115 120 125 gat gct ggt att gat atc aac ggc gct gat tta agc cg ggt gta 432 Asp Ala Gly Ile Asp Ile Asn Gly Ala Asp Leu Ser Arg Ala Gly Val 130 135 140 gtc atg ggc gcg ctg tcg ttc cca act acc cgc tca aac gat ctg ttt 480 Val Met Gly Ala Leu Ser Phe Pro Thr Thrg Ser Asn Asp Leu Phe 145 150 155 160 ttg cca att tat cac agc gcc gtt gaa aaa gcc ctg caa gat aaa cta 528 Leu Pro Ile Tyr His Ser Ala Val Glu Lys Ala Leu Gln Asp Lys Leu 165 170 175 ggc gta aag gcatt aag cta agc cca act aat gct cat acc gct cgc 576 Gly Val Lys Ala Phe Lys Leu Ser Pro Thr Asn Ala His Thr Ala Arg 180 185 190 gcg gca aat gag agc agc cta aat gca gcc aat ggt gcc att gcc cat 624 Ala Ala Asn Glu Ser Ser Leu Asn Ala Ala Asn Gly Ala Ile Ala His 195 200 205 aac agc tca aaa gtg gtg gcc gat gca ctt ggc ctt ggc ggc gca caa 672 Asn Ser Ser Lys Val Val Ala Asp Ala Leu Gly Leu Gly Gly Ala Gln 210 215 220 cta agc cta gat gct gcc tgt gct agt tcg gtt tac tca tta aag ctt 720 Leu Ser Leu Asp Ala Ala Cys Ala Ser Ser Val Tyr Ser Leu Lys Leu 225 230 235 240 gcc tgc gat tac cta agc act ggc aaa gcc gat atc atg cta gca ggc 768 Ala Cys Asp Tyr Leu Ser Thr Gly Lys Ala Asp Ile Met Leu Ala Gly 245 250 255 gca gta tct ggc gcg gat cct ttc ttt att aat atg gga ttc tca atc 816 Ala Val Ser Gly Ala Pro Phe Phe Ile Asn Met Gly Phe Ser Ile 260 265 270 ttc cac gcc tac cca gac cat ggt atc tca gta ccg ttt gat gcc agc 864 Phe His Ala Tyr Pro Asp His Gly Ile Ser Val Pro Phe Asp Ala Ser 275 280 285 agt aaa ggt ttg ttt gct ggc gaa ggc gct ggc gta tta gtg ctt aaa 912 Ser Lys Gly Leu Phe Ala Gly Glu Gly Ala Gly Val Leu Val Leu Lys 290 295 300 cgt ctt gaa gat gcc gag cgc gac agat gac gat gtt 960 Arg Leu Glu Asp Ala Glu Arg Asp Asn Asp Lys Ile Tyr Ala Val Val 305 310 315 320 agc ggc gta ggt cta tca aac gac ggt aaa ggc cag ttt gta tta agc 1008 Ser Gly Val Gly Leu Ser Asn Asp Gly Lys Gl n Phe Val Leu Ser 325 330 335 cct aat cca aaa ggt cag gtg aag gcc ttt gaa cgt gct tat gct gcc 1056 Pro Asn Pro Lys Gly Gln Val Lys Ala Phe Glu Arg Ala Tyr Ala Ala 340 345 350 agt gac att gag cca aaa gac att gaa gtg att gag tgc cac gca aca 1104 Ser Asp Ile Glu Pro Lys Asp Ile Glu Val Ile Glu Cys His Ala Thr 355 360 365 ggc aca ccg ctt ggc gat aaa att gag ctc act tca atg gaa acc ttc 1152 Gly Thr Leu Gly Asp Lys Ile Glu Leu Thr Ser Met Glu Thr Phe 370 375 380 ttt gaa gac aag ctg caa ggc acc gat gca ccg tta att ggc tca gct 1200 Phe Glu Asp Lys Leu Gln Gly Thr Asp Ala Pro Leu Ile Gly Ser Ala 385 390 395 400 aag tct aac tta ggc cac cta tta act gca gcg ggc atg ccg ggg atc 1248 Lys Ser Asn Leu Gly His Leu Leu Thr Ala Ala Gly Met Pro Gly Ile 405 410 415 atg aag atg atc ttc gcc atg aaa gaa ggt ctg ccg cca agt atc 1296 Met Lys Met Ile Phe Ala Met Lys Glu Gly Tyr Leu Pro Pro Ser Ile 420 425 430 aat att agt gat gct atc gct tcg ccg aaa aaa ctc ttc ggt aaa cca 1344 Asn Ile Ser Asp Ala Ile A Ser Pro Lys Lys Leu Phe Gly Lys Pro 435 440 445 acc ctg cct agc atg gtt caa ggc tgg cca gat aag cca tcg aat aat 1392 Thr Leu Pro Ser Met Val Gln Gly Trp Pro Asp Lys Pro Ser Asn Asn 450 455 460 cat ttt ggt gta aga acc cgt cac gca ggc gta tcg gta ttt ggc ttt 1440 His Phe Gly Val Arg Thr Arg His Ala Gly Val Ser Val Phe Gly Phe 465 470 475 ggt ggc tgt aac gcc cat ctg ttg ctt gag tca tac aac ggc ga g 1488 Gly Gly Cys Asn Ala His Leu Leu Leu Glu Ser Tyr Asn Gly Lys Gly 480 485 490 495 aca gta aag gca gaa gcc act caa gta ccg cgt caa gct gag ccg cta 1536 Thr Val Lys Ala Glu Ala Thr Gln Val Pro Arg Gln Ala Glu Pro Leu 500 505 510 aaa gtg gtt ggc ctt gcc tcg cac ttt ggg cct ctt agc agc att aat 1584 Lys Val Val Gly Leu Ala Ser His Phe Gly Pro Leu Ser Ser Ile Asn 515 520 525 gca ctc aac aat gct gtg acc caa gat ggg aat ggc ttt atc gaa ctg 1632 Ala Leu Asn Asn Ala Val Thr Gln Asp Gly Asn Gly Phe Ile Glu Leu 530 535 540 ccg aaa aag cgc tgg aaa ggc ctt gaa aag cac agt gag ctg tta gct 1 Lys Arg Trp Lys Gly Leu Glu Lys His Ser Glu Leu Leu Ala 545 550 555 gaa ttt ggc tta gca tct gcg cca aaa ggt gct tat gtt gat aac ttc 1728 Glu Phe Gly Leu Ala Ser Ala Pro Lys Gly Ala Tyr Val Asp Asn 560 565 570 575 gag ctg gac ttt tta cgc ttt aaa ctg ccg cca aac gaa gat gac cgt 1776 Glu Leu Asp Phe Leu Arg Phe Lys Leu Pro Pro Asn Glu Asp Asp Arg 580 585 590 ttg atc tca cag cg ctag atg gta aca gac gaa gcc att 1824 Leu Ile Ser Gln Gln Leu Met Leu Met Arg Val Thr Asp Glu Ala Ile 595 600 605 cgt gat gcc aag ctt gag ccg ggg caa aaa gta gct gta tta gtg gca 1872 Arg Asp Ala Lys Leu Glu Gly Gln Lys Val Ala Val Leu Val Ala 610 615 620 atg gaa act gag ctt gaa ctg cat cag ttc cgc ggc cgg gtt aac ttg 1920 Met Glu Thr Glu Leu Glu Leu His Gln Phe Arg Gly Arg Val Asn Leu 625 630 635 cat act caa tta gcg caa agt ctt gcc gcc atg ggc gtg agt tta tca 1968 His Thr Gln Leu Ala Gln Ser Leu Ala Ala Met Gly Val Ser Leu Ser 640 645 650 655 655 acg gat gaa tac caa gcg ctt gaa gcc atc gcc atg gac ag c gtg ctt 2016 Thr Asp Glu Tyr Gln Ala Leu Glu Ala Ile Ala Met Asp Ser Val Leu 660 665 670 gat gct gcc aag ctc aat cag tac acc agc ttt att ggt aat att atg 2064 Asp Ala Ala Lys Leu Asn Gln Tyr Thr Ser Phe Ile Gly Asn Ile Met 675 680 685 gcg tca cgc gtg gcg tca cta tgg gac ttt aat ggc cca gcc ttc act 2112 Ala Ser Arg Val Ala Ser Leu Trp Asp Phe Asn Gly Pro Ala Phe Thr 690 695 700 att tca gca gca caa tct gtg agc cgc tgt atc gat gtg gcg caa 2160 Ile Ser Ala Ala Glu Gln Ser Val Ser Arg Cys Ile Asp Val Ala Gln 705 710 715 aac ctc atc atg gag gat aac cta gat gcg gtg gtg Att gca gc Asg Ile Met Glu Asp Asn Leu Asp Ala Val Val Ile Ala Ala Val 720 725 730 735 gat ctc tct ggt agc ttt gag caa gtc att ctt aaa aat gcc att gca 2256 Asp Leu Ser Gly Ser Phe Glu Gln Val Ile Leu Lys Asn Ala Ile Ala 740 745 750 cct gta gcc att gag cca aac ctc gaa gca agc ctt aat cca aca tca 2304 Pro Val Ala Ile Glu Pro Asn Leu Glu Ala Ser Leu Asn Pro Thr Ser 755 760 765 gca agc tgg aat gtc ggt gaa ggt gc t ggc gcg gtc gtg ctt gtt aaa 2352 Ala Ser Trp Asn Val Gly Glu Gly Ala Gly Ala Val Val Leu Val Lys 770 775 780 aat gaa gct aca tcg ggc tgc tca tac ggc caa att gat gca ctt ggc 2400 Asn Gly Cys Ser Tyr Gly Gln Ile Asp Ala Leu Gly 785 790 795 ttt gct aaa act gcc gaa aca gcg ttg gct acc gac aag cta ctg agc 2448 Phe Ala Lys Thr Ala Glu Thr Ala Leu Ala Thr Asp Lys Leu Leu Ser 800 805 815 caa act gcc aca gac ttt aat aag gtt aaa gtg att gaa act atg gca 2496 Gln Thr Ala Thr Asp Phe Asn Lys Val Lys Val Ile Glu Thr Met Ala 820 825 830 gcg cct gct agc caa att caa tta gcg cca atg gtt tct caa gtg 2544 Ala Pro Ala Ser Gln Ile Gln Leu Ala Pro Ile Val Ser Ser Gln Val 835 840 845 act cac act gct gca gag cag cgt gtt ggt cac tgc ttt gct gca gcg 2592 Thr His Thr Ala Ala Glu Gln Arg Val Gly His Cys Phe Ala Ala Ala 850 855 860 ggt atg gca agc cta tta cac ggc tta ctt aac tta aat act gta gcc 2640 Gly Met Ala Ser Leu Leu Leu His Gly Leu Leu Asn Leu Asn Thr Val Ala 865 870 875 caa acc aat aaa gcc aat tgc gcg ctt atc aac aat atc agt gaa aac 2688 Gln Thr Asn Lys Ala Asn Cys Ala Leu Ile Asn Asn Ile Ser Glu Asn 880 885 890 895 caa tta tca cag ctg ttg att agc caa aca gcg caca gca gca gca gca gca caca Gln Leu Ser Gln Leu Leu Ile Ser Gln Thr Ala Ser Glu Gln Gln Ala 900 905 910 tta acc gcg cgt tta agc aat gag ctt aaa tcc gat gct aaa cac caa 2784 Leu Thr Ala Arg Leu Ser Asn Glu Leu Lys Ser Asp Ala Lys His Gln 915 920 925 ctg gtt aag caa gtc acc tta ggt ggc cgt gat atc tac cag cat att 2832 Leu Val Lys Gln Val Thr Leu Gly Gly Arg Asp Ile Tyr Gln His Ile 930 935 940 gtt gat aca ccg ctt gca agc cttga agc att act cag aaa ttg gcg 2880 Val Asp Thr Pro Leu Ala Ser Leu Glu Ser Ile Thr Gln Lys Leu Ala 945 950 955 caa gcg aca gca tcg aca gtg gtc aac caa gtt aaa cct att aag gcc 2928 Gln Ala Thr Ala Ser Thr Val Val Asn Gln Val Lys Pro Ile Lys Ala 960 965 970 975 gct ggc tca gtc gaa atg gct aac tca ttc gaa acg gaa agc tca gca 2976 Ala Gly Ser Val Glu Met Ala Asn Ser Phe Glu Thr Glu Ser Ser Ala 980 985 990 gag cca caa ata aca att gca gca caa cag act gca aac att ggc gtc 3024 Glu Pro Gln Ile Thr Ile Ala Ala Gln Gln Thr Ala Asn Ile Gly Val 995 1000 1005 acc gct cag gca acc aaa cgt gaa tta ggt acc cca cca atg aca aca 3072 Thr Ala Gln Ala Thr Lys Arg Glu Leu Gly Thr Pro Pro Met Thr Thr 1010 1015 1020 aat acc att gct aat aca gca aat aat tta gac aag act ctt gag act 3120 Asn Thr Ile Ala Asn Thr Ala Asn Asn Leu Asp Lys Thr Leu Glu Thr 1025 1030 1035 gtt gct ggc aat act gtt gct agc aag gtt ggc tct ggc gac ata gtc 3168 Val Ala Gly Asn Thr Val Ala Ser Lys Val Gly Ser Gly Asp Ile Val 1040 1045 1050 1055 aat ttt caa cag aac caa caa ttg gct caa caa gct cac ctc gcc ttt 3216 Asn Phe Gln Gln Asn Gln Gln Leu Ala Gln Gln Ala His Leu Ala Phe 1060 1065 1070 ctt gaa agc cgc agt gcg ggt atg aag gtg gct gat ag Leu Glu Ser Arg Ser Ala Gly Met Lys Val Ala Asp Ala Leu Leu Lys 1075 1080 1085 caa cag cta gct caa gta aca ggc caa act atc gat aat cag gcc ctc 3312 Gln Gln Leu Ala Gln Val Thr Gl y Gln Thr Ile Asp Asn Gln Ala Leu 1090 1095 1100 gat act caa gcc gtc gat act caa aca agc gag aat gta gcg att gcc 3360 Asp Thr Gln Ala Val Asp Thr Gln Thr Ser Glu Asn Val Ala Ile Ala 1105 1110 1115 gca gaa tca cca gtt caa gtt aca aca cct gtt caa gtt aca aca cct 3408 Ala Glu Ser Pro Val Gln Val Thr Thr Pro Val Gln Val Thr Thr Pro 1120 1125 1130 1135 gtt caa atc agt gtt gtg gag tta aaa cca gat cac gct aat gtg cca 3456 Val Gln Ile Ser Val Val Glu Leu Lys Pro Asp His Ala Asn Val Pro 1140 1145 1150 cca tac acg ccg cca gtg cct gca tta aag ccg tgt atc tgg aac tat 3504 Pro Tyr Thr Pro Pro Val Pro Ala Leu Lys Pro Cys Ile Trp Asn Tyr 1155 1160 1165 gcc gat tta gtt gag tac gca gaa ggc gat atc gcc aag gta ttt ggc 3552 Ala Asp Leu Val Glu Tyr Ala Glu Gly Asp Ile Ala Lys Val Phe Gly 1170 1175 1180 agt gat atc gat atc gat agc tac tcg cgc cgc gta cgt cta ccg 3600 Ser Asp Tyr Ala Ile Ile Asp Ser Tyr Ser Arg Arg Val Arg Leu Pro 1185 1190 1195 acc act gac tac ctg ttg gta tcg cgc gtg acc aaa ctt gat gc g acc 3648 Thr Thr Asp Tyr Leu Leu Val Ser Arg Val Thr Lys Leu Asp Ala Thr 1200 1205 1210 1215 atc aat caa ttt aag cca tgc tca atg acc act gag tac gac atc cct 3696 Ile Asn Gln Phe Lys Pro Cys Ser Met Thr Thr Glu Tyr Asp Ile Pro 1220 1225 1230 gtt gat gcg ccg tac tta gta gac gga caa atc cct tgg gcg gta gca 3744 Val Asp Ala Pro Tyr Leu Val Asp Gly Gln Ile Pro Trp Ala Val Ala 1235 1240 1245 gta gaa tca ggc caa tgt gac ttg atg ctt att agc tat ctc ggt atc 3792 Val Glu Ser Gly Gln Cys Asp Leu Met Leu Ile Ser Tyr Leu Gly Ile 1250 1255 1260 gac ttt gag aac aaa ggc gag cgg gtt tat cga cta ctc gat tgt acc 3840 Glu Asn Lys Gly Glu Arg Val Tyr Arg Leu Leu Asp Cys Thr 1265 1270 1275 ctc acc ttc cta ggc gac ttg cca cgt ggc gga gat acc cta cgt tac 3888 Leu Thr Phe Leu Gly Asp Leu Pro Arg Gly Gly Asp Thr Leu Arg 1280 1285 1290 1295 gac att aag atc aat aac tat gct cgc aac ggc gac acc ctg ctg ttc 3936 Asp Ile Lys Ile Asn Asn Tyr Ala Arg Asn Gly Asp Thr Leu Leu Phe 1300 1305 1310 ttc ttc tcg tat gag tgt ttt gtt ggc gac aag atg atc ctc aag atg 3984 Phe Phe Ser Tyr Glu Cys Phe Val Gly Asp Lys Met Ile Leu Lys Met 1315 1320 1325 gat ggc ggc tgc gct ggc ttc ttc act gat gaga gag gtt Asp Gly Gly Cys Ala Gly Phe Phe Thr Asp Glu Glu Leu Ala Asp Gly 1330 1335 1340 aaa ggc gtg att cgc aca gaa gaa gag att aaa gct cgc agc cta gtg 4080 Lys Gly Val Ile Arg Thr Glu Glu Glu Ig Leu Val 1345 1350 1355 caa aag caa cgc ttt aat ccg tta cta gat tgt cct aaa acc caa ttt 4128 Gln Lys Gln Arg Phe Asn Pro Leu Leu Asp Cys Pro Lys Thr Gln Phe 1360 1365 1370 1375 agt tat ggt gat att cat aag cta tta act gct gat att gag ggt tgt 4176 Ser Tyr Gly Asp Ile His Lys Leu Leu Thr Ala Asp Ile Glu Gly Cys 1380 1385 1390 ttt ggc cca agc cac agt ggc gtc cac cag ccg tca ctt tgt ttc gca 4224 Phe Gly Ser Ser Gly Val His Gln Pro Ser Leu Cys Phe Ala 1395 1400 1405 tct gaa aaa ttc ttg atg att gaa caa gtc agc aag gtt gat cgc act 4272 Ser Glu Lys Phe Leu Met Ile Glu Gln Val Ser Lys Va l Asp Arg Thr 1410 1415 1420 ggc ggt act tgg gga ctt ggc tta att gag ggt cat aag cag ctt gaa 4320 Gly Gly Thr Trp Gly Leu Gly Leu Ile Glu Gly His Lys Gln Leu Glu 1425 1430 1435 gca gac cac tgg tac ttcc tgt cat ttc aag ggc gac caa gtg atg 4368 Ala Asp His Trp Tyr Phe Pro Cys His Phe Lys Gly Asp Gln Val Met 1440 1445 1450 1455 gct ggc tcg cta atg gct gaa ggt tgt ggc cag tta ttg cag ttc Gtat 4416 Leu Met Ala Glu Gly Cys Gly Gln Leu Leu Gln Phe Tyr 1460 1465 1470 atg ctg cac ctt ggt atg cat acc caa act aaa aat ggt cgt ttc caa 4464 Met Leu His Leu Gly Met His Thr Gln Thr Lys Asn Gly Arg Phe Gln 1475 1480 1485 cct ctt gaa aac gcc tca cag caa gta cgc tgt cgc ggt caa gtg ctg 4512 Pro Leu Glu Asn Ala Ser Gln Gln Val Arg Cys Arg Gly Gln Val Leu 1490 1495 1500 cca caa tca ggc gtg cta act tac cgtg act gaa atc ggt 4560 Pro Gln Ser Gly Val Leu Thr Tyr Arg Met Glu Val Thr Glu Ile Gly 1505 1510 1515 ttc agt cca cgc cca tat gct aaa gct aac atc gat atc ttg ctt aat 4608 Phe Ser Pro Arg Pro Tyr Ala Lys Ala Asn Ile Asp Ile Leu Leu Asn 1520 1525 1530 1535 ggc aaa gcg gta gtg gat ttc caa aac cta ggg gtg atg ata aaa gag 4656 Gly Lys Ala Val Val Asp Phe Gln Asn Leu Gly Val Met Glu 1540 1545 1550 gaa gat gag tgt act cgt tat cca ctt ttg act gaa tca aca acg gct 4704 Glu Asp Glu Cys Thr Arg Tyr Pro Leu Leu Thr Glu Ser Thr Thr Ala 1555 1560 1565 agc act gca caa gta aac gct caa aca agt gcg aaa aag gta tac aag 4752 Ser Thr Ala Gln Val Asn Ala Gln Thr Ser Ala Lys Lys Val Tyr Lys 1570 1575 1580 cca gca tca gtc aat gcg cca tta atg gca caa att cct gat ctg act 4800 Pro Ala Ser Val Asn Ala Pro Leu Met Ala Gln Ile Pro Asp Leu Thr 1585 1590 1595 aaa gag cca aac aag ggc gtt att ccg att tcc cat gtt gaa gca cca 4848 Lys Glu Pro Asn Lys Gly Val Ile Pro Ile Ser His Val Glu Ala Pro 1600 1605 1610 1615 att acg cca gac tac ccg aac cgt gta cct gat aca gtg cca ttc acg 4896 Ile Thr Pro Asp Tyr Pro Asn Arg Val Pro Asp Thr Val Pro Phe Thr 1620 1625 1630 ccg tat cac atg ttt gag ttt gct aca ggc aat atc gaa aac tgt ttc 4944 Pro Tyr His Met Phe Glu Phe Ala Thr Gly Asn Ile Glu Asn Cys Phe 1635 1640 1645 ggg cca gag ttc tca atc tat cgc ggc atg atc cca cca cgt aca cca 4992 Gly Pro Glu Ile Tyr Arg Gly Met Ile Pro Pro Arg Thr Pro 1650 1655 1660 tgc ggt gac tta caa gtg acc aca cgt gtg att gaa gtt aac ggt aag 5040 Cys Gly Asp Leu Gln Val Thr Thr Arg Val Ile Glu Val Asn Gly Lys 1665 1670 1675 cgt ggc gac ttt aaa aag cca tca tcg tgt atc gct gaa tat gaa gtg 5088 Arg Gly Asp Phe Lys Lys Pro Ser Ser Cys Ile Ala Glu Tyr Glu Val 1680 1685 1690 1695 cct gca gat gcg tgg tat ttc gat cac aa aac aac gca gtg atg 5136 Pro Ala Asp Ala Trp Tyr Phe Asp Lys Asn Ser His Gly Ala Val Met 1700 1705 1710 cca tat tca att tta atg gag atc tca ctg caa cct aac ggc ttt atc 5184 Pro Tyr Ser Ile Leu Met Glu Ile Ser Leu Gln Pro Asn Gly Phe Ile 1715 1720 1725 tca ggt tac atg ggc aca acc cta ggc ttc cct ggc ctt gag ctg ttc 5232 Ser Gly Tyr Met Gly Thr Thr Leu Gly Phe Pro Gly Leu Glu Leu Phe 1730 1735 1740 ttc cgt aac tta gac ggt agc ggt gag tta cta cgt gaa gta gat tta 5280 Phe Arg Asn Leu Asp Gly Ser Gly Glu Leu Leu Arg Glu Val Asp Leu 1745 1750 1755 cgt ggt aaa acc atc cgt aac gac tca cgt tca aca gtg atg 5328 Arg Gly Lys Thr Ile Arg Asn Asp Ser Arg Leu Leu Ser Thr Val Met 1760 1765 1770 1775 gcc ggc act aac atc atc caa agc ttt agc ttc gag cta agc act gac 5376 Ala Gly Thr Asn Ile Ile Gln Ser Phe Ser Phe Glu Leu Ser Thr Asp 1780 1785 1790 ggt gag cct ttc tat cgc ggc act gcg gta ttt ggc tat ttt aaa ggt 5424 Gly Glu Pro Phe Tyr Arg Gly Thr Ala Val Phe Gly Tyr Phe Lys Gly 1795 1800 1805 gac gtt aaa gat cag cta ggc cta gat aac ggt aaa gtc act cag 5472 Asp Ala Leu Lys Asp Gln Leu Gly Leu Asp Asn Gly Lys Val Thr Gln 1810 1815 1820 cca tgg cat gta gct aac ggc gtt gct gca agc act aag gtg aac gt Pro Trp His Val Ala Asn Gly Val Ala Ala Ser Thr Lys Val Asn Leu 1825 1830 1835 ctt gat aag agc tgc cgt cac ttt aat gcg cca gct aac cag cca cac 5568 Leu Asp Lys Ser Cys Arg His Phe Asn Ala Pro Ala Asn Gln Pro His 1840 1845 1850 1855 tat cgt cta gcc ggt ggt cag ctg aac ttt atc gac agt gtt gaa att 5616 Tyr Arg Leu Ala Gly Gly Gln Leu Asn Phe Ile Asp Ser Val Glu Ile 1860 1865 1870 gtt gat aat ggc ggc acc gaa ggt tta ggt tac ttg tat gcc gag cgc 5664 Val Asp Asn Gly Gly Thr Glu Gly Leu Gly Tyr Leu Tyr Ala Glu Arg 1875 1880 1885 acc att gac cca agt gat tgg ttc ttc cag ttc cac ttc cac cttc gat 5712 Thr Ile Asp Pro Ser Asp Trp Phe Phe Gln Phe His Phe His Gln Asp 1890 1895 1900 ccg gtt atg cca ggc tcc tta ggt gtt gaa gca att att gaa acc atg 5760 Pro Val Met Pro Gly Ser Leu Gly Val Glu Ala Ile Ile Glu Thr Met 1905 1910 1915 caa gct tac gct att agt aaa gac ttg ggc gca gat ttc aaa aat cct 5808 Gln Ala Tyr Ala Ile Ser Lys Asp Leu Gly Ala Asp Phe Lys Asn Pro 1920 1925 1930 1935 aag ttt ggt cagt att tcg aac atc aag tgg aag tat cgc ggt caa 5856 Lys Phe Gly Gln Ile Leu Ser Asn Ile Lys Trp Lys Tyr Arg Gly Gln 1940 1945 1950 atc aat ccg ctg aac aag cag atg tct atg gat gtc ag c att act tca 5904 Ile Asn Pro Leu Asn Lys Gln Met Ser Met Asp Val Ser Ile Thr Ser 1955 1960 1965 atc aaa gat gaa gac ggt aag aaa gtc atc aca ggt aat gcc agc ttg 5952 Ile Lys Asp Glu Asp Gly Lys Lys Val Ile Thr Gly Asn Ala Ser Leu 1970 1975 1980 agt aaa gat ggt ctg cgc ata tac gag gtc ttc gat ata gct atc agc 6000 Ser Lys Asp Gly Leu Arg Ile Tyr Glu Val Phe Asp Ile Ala Ile Ser 1985 1990 1995 atc gaa gaa gta 6015 Ile Glu Glu Ser Val 2000 <210> 11 <211> 2005 <212> PRT <400> 1 Met Ser Leu Pro Asp Asn Ala Ser Asn His Leu Ser Ala Asn Gln Lys 1 5 10 15 Gly Ala Ser Gln Ala Ser Lys Thr Ser Lys Gln Ser Lys Ile Ala Ile 20 25 30 Val Gly Leu Ala Thr Leu Tyr Pro Asp Ala Lys Thr Pro Gln Glu Phe 35 40 45 Trp Gln Asn Leu Leu Asp Lys Arg Asp Ser Arg Ser Thr Leu Thr Asn 50 55 60 Glu Lys Leu Gly Ala Asn Ser Gln Asp Tyr Gln Gly Val Gln Gly Gln 65 70 75 80 Ser Asp Arg Phe Tyr Cys Asn Lys Gly Gly Tyr Ile Glu Asn Phe Ser 85 90 95 Phe Asn Ala Ala Gly Tyr Lys Leu Pro Glu Gln Ser Leu Asn Gly Leu 100 105 110 Asp Asp Ser Phe Leu Trp Ala Leu Asp Thr Ser Arg Asn Ala Leu Ile 115 120 125 Asp Ala Gly Ile Asp Ile Asn Gly Ala Asp Leu Ser Arg Ala Gly Val 130 135 140 Val Met Gly Ala Leu Ser Phe Pro Thr Thr Arg Ser Asn Asp Leu Phe 145 150 155 160 Leu Pro Ile Tyr His Ser Ala Val Glu Lys Ala Leu Gln Asp Lys Leu 165 170 175 Gly Val Lys Ala Phe Lys Leu Ser Pro Thr Asn Ala His Thr Ala Arg 180 185 190 Ala Ala Asn Glu Ser Ser Leu Asn Ala Ala Asn Gly Ala Ile Ala His 195 200 205 Asn Ser Ser L ys Val Val Ala Asp Ala Leu Gly Leu Gly Gly Ala Gln 210 215 220 Leu Ser Leu Asp Ala Ala Cys Ala Ser Ser Val Tyr Ser Leu Lys Leu 225 230 235 240 Ala Cys Asp Tyr Leu Ser Thr Gly Lys Ala Asp Ile Met Leu Ala Gly 245 250 255 Ala Val Ser Gly Ala Asp Pro Phe Phe Ile Asn Met Gly Phe Ser Ile 260 265 270 Phe His Ala Tyr Pro Asp His Gly Ile Ser Val Pro Phe Asp Ala Ser 275 280 285 Ser Lys Gly Leu Phe Ala Gly Glu Gly Ala Gly Val Leu Val Leu Lys 290 295 300 Arg Leu Glu Asp Ala Glu Arg Asp Asn Asp Lys Ile Tyr Ala Val Val 305 310 315 320 Ser Gly Val Gly Leu Ser Asn Asp Gly Lys Gly Gln Phe Val Leu Ser 325 330 335 Pro Asn Pro Lys Gly Gln Val Lys Ala Phe Glu Arg Ala Tyr Ala Ala 340 345 350 Ser Asp Ile Glu Pro Lys Asp Ile Glu Val Ile Glu Cys His Ala Thr 355 360 365 Gly Thr Pro Leu Gly Asp Lys Ile Glu Leu Thr Ser Met Glu Thr Phe 370 375 380 Phe Glu Asp Lys Leu Gln Gly Thr Asp Ala Pro Leu Ile Gly Ser Ala 385 390 395 400 Lys Ser Asn Leu Gly His Leu Leu Thr Ala Ala Gly Met Pro Gly Ile 405 410 415 Met Lys MetIle Phe Ala Met Lys Glu Gly Tyr Leu Pro Pro Ser Ile 420 425 430 Asn Ile Ser Asp Ala Ile Ala Ser Pro Lys Lys Leu Phe Gly Lys Pro 435 440 445 Thr Leu Pro Ser Met Val Gln Gly Trp Pro Asp Lys Pro Ser Asn Asn 450 455 460 His Phe Gly Val Arg Thr Arg His Ala Gly Val Ser Val Phe Gly Phe 465 470 475 480 Gly Gly Cys Asn Ala His Leu Leu Leu Glu Ser Tyr Asn Gly Lys Gly 485 490 490 495 Thr Val Lys Ala Glu Ala Thr Gln Val Pro Arg Gln Ala Glu Pro Leu 500 505 510 Lys Val Val Gly Leu Ala Ser His Phe Gly Pro Leu Ser Ser Ile Asn 515 520 525 Ala Leu Asn Asn Ala Val Thr Gln Asp Gly Asn Gly Phe Ile Glu Leu 530 535 540 Pro Lys Lys Arg Trp Lys Gly Leu Glu Lys His Ser Glu Leu Leu Ala 545 550 555 560 Glu Phe Gly Leu Ala Ser Ala Pro Lys Gly Ala Tyr Val Asp Asn Phe 565 570 575 Glu Leu Asp Phe Leu Arg Phe Lys Leu Pro Pro Asn Glu Asp Asp Arg 580 585 590 Leu Ile Ser Gln Gln Leu Met Leu Met Arg Val Thr Asp Glu Ala Ile 595 600 605 Arg Asp Ala Lys Leu Glu Pro Gly Gln Lys Val Ala Val Leu Val Ala 610 615 615 620 Met Glu Thr GluLeu Glu Leu His Gln Phe Arg Gly Arg Val Asn Leu 625 630 635 640 His Thr Gln Leu Ala Gln Ser Leu Ala Ala Met Gly Val Ser Leu Ser 645 650 655 Thr Asp Glu Tyr Gln Ala Leu Glu Ala Ile Ala Met Asp Ser Val Leu 660 665 670 Asp Ala Ala Lys Leu Asn Gln Tyr Thr Ser Phe Ile Gly Asn Ile Met 675 680 685 Ala Ser Arg Val Ala Ser Leu Trp Asp Phe Asn Gly Pro Ala Phe Thr 690 695 700 Ile Ser Ala Ala Glu Gln Ser Val Ser Arg Cys Ile Asp Val Ala Gln 705 710 715 715 720 Asn Leu Ile Met Glu Asp Asn Leu Asp Ala Val Val Ile Ala Ala Val 725 730 735 Asp Leu Ser Gly Ser Phe Glu Gln Val Ile Leu Lys Asn Ala Ile Ala 740 745 750 Pro Val Ala Ile Glu Pro Asn Leu Glu Ala Ser Leu Asn Pro Thr Ser 755 760 765 Ala Ser Trp Asn Val Gly Glu Gly Ala Gly Ala Val Val Leu Val Lys 770 775 780 780 Asn Glu Ala Thr Ser Gly Cys Ser Tyr Gly Gln Ile Asp Ala Leu Gly 785 790 795 800 Phe Ala Lys Thr Ala Glu Thr Ala Leu Ala Thr Asp Lys Leu Leu Ser 805 810 815 Gln Thr Ala Thr Asp Phe Asn Lys Val Lys Val Ile Glu Thr Met Ala 820 825 830 Ala Pro Ala Ser Gln Ile Gln Leu Ala Pro Ile Val Ser Ser Gln Val 835 840 845 Thr His Thr Ala Ala Glu Gln Arg Val Gly His Cys Phe Ala Ala Ala 850 855 860 Gly Met Ala Ser Leu Leu His Gly Leu Leu Asn Leu Asn Thr Val Ala 865 870 875 880 Gln Thr Asn Lys Ala Asn Cys Ala Leu Ile Asn Asn Ile Ser Glu Asn 885 890 895 Gln Leu Ser Gln Leu Leu Ile Ser Gln Thr Ala Ser Glu Gln Gln Ala 900 905 910 910 Leu Thr Ala Arg Leu Ser Asn Glu Leu Lys Ser Asp Ala Lys His Gln 915 920 925 925 Leu Val Lys Gln Val Thr Leu Gly Gly Arg Asp Ile Tyr Gln His Ile 930 935 940 Val Asp Thr Pro Leu Ala Ser Leu Glu Ser Ile Thr Gln Lys Leu Ala 945 950 955 960 Gln Ala Thr Ala Ser Thr Val Val Asn Gln Val Lys Pro Ile Lys Ala 965 970 975 Ala Gly Ser Val Glu Met Ala Asn Ser Phe Glu Thr Glu Ser Ser Ala 980 985 990 Glu Pro Gln Ile Thr Ile Ala Ala Gln Gln Thr Ala Asn Ile Gly Val 995 1000 1005 Thr Ala Gln Ala Thr Lys Arg Glu Leu Gly Thr Pro Pro Met Thr 1010 1015 1020 Asn Thr Ile Ala Asn Thr Ala Asn Asn Leu Asp Lys Thr Leu Glu Thr 1025 1030 1035 1040 Val Al a Gly Asn Thr Val Ala Ser Lys Val Gly Ser Gly Asp Ile Val 1045 1050 1055 Asn Phe Gln Gln Asn Gln Gln Leu Ala Gln Gln Ala His Leu Ala Phe 1060 1065 1070 Leu Glu Ser Arg Ser Ala Gly Met Lys Val Ala Asp Ala Leu Leu Lys 1075 1080 1085 Gln Gln Leu Ala Gln Val Thr Gly Gln Thr Ile Asp Asn Gln Ala Leu 1090 1095 1100 Asp Thr Gln Ala Val Asp Thr Gln Thr Ser Glu Asn Val Ala Ile Ala 1105 1110 1115 1120 Ala Glu Ser Pro Val Gln Val Thr Thr Pro Val Gln Val Thr Thr Pro 1125 1130 1135 Val Gln Ile Ser Val Val Glu Leu Lys Pro Asp His Ala Asn Val Pro 1140 1145 1150 Pro Tyr Thr Pro Pro Val Pro Ala Leu Lys Pro Cys Ile Trp Asn Tyr 1155 1160 1165 Ala Asp Leu Val Glu Tyr Ala Glu Gly Asp Ile Ala Lys Val Phe Gly 1170 1175 1180 Ser Asp Tyr Ala Ile Ile Asp Ser Tyr Ser Arg Arg Val Arg Leu Pro 1185 1190 1195 1200 Thr Thr Asp Tyr Leu Leu Val Ser Arg Val Thr Lys Leu Asp Ala Thr 1205 1210 1215 Ile Asn Gln Phe Lys Pro Cys Ser Met Thr Thr Glu Tyr Asp Ile Pro 1220 1225 1230 Val Asp Ala Pro Tyr Leu Val Asp Gly Gln Ile Pro Trp Ala Val Ala 1235 1240 1245 Val Glu Ser Gly Gln Cys Asp Leu Met Leu Ile Ser Tyr Leu Gly Ile 1250 1255 1260 Asp Phe Glu Asn Lys Gly Glu Glu Arg Val Tyr Arg Leu Leu Asp Cys Thr 1265 1270 1275 1280 Leu Thr Phe Leu Gly Asp Leu Pro Arg Gly Gly Asp Thr Leu Arg Tyr 1285 1290 1295 Asp Ile Lys Ile Asn Asn Tyr Ala Arg Asn Gly Asp Thr Leu Leu Phe 1300 1305 1310 Phe Phe Ser Tyr Glu Cys Phe Val Gly Asp Lys Met Ile Leu Lys Met 1315 1320 1325 Asp Gly Gly Cys Ala Gly Phe Phe Thr Asp Glu Glu Leu Ala Asp Gly 1330 1335 1340 Lys Gly Val Ile Arg Thr Glu Glu Glu Ile Lys Ala Arg Ser Leu Val 1345 1350 1355 1360 Gln Lys Gln Arg Phe Asn Pro Leu Leu Asp Cys Pro Lys Thr Gln Phe 1365 1370 1375 Ser Tyr Gly Asp Ile His Lys Leu Leu Thr Ala Asp Ile Glu Gly Cys 1380 1385 1390 Phe Gly Pro Ser His Ser Gly Val His Gln Pro Ser Leu Cys Phe Ala 1395 1400 1405 Ser Glu Lys Phe Leu Met Ile Glu Gln Val Ser Lys Val Asp Arg Thr 1410 1415 1420 Gly Gly Thr Trp Gly Leu Gly Leu Ile Glu Gly His Lys Gln Leu Glu 1425 1430 1435 1440 Ala A sp His Trp Tyr Phe Pro Cys His Phe Lys Gly Asp Gln Val Met 1445 1450 1455 Ala Gly Ser Leu Met Ala Glu Gly Cys Gly Gln Leu Leu Gln Phe Tyr 1460 1465 1470 Met Leu His Leu Gly Met His Thr Gln Thr Lys Asn Gly Arg Phe Gln 1475 1480 1485 Pro Leu Glu Asn Ala Ser Gln Gln Val Arg Cys Arg Gly Gln Val Leu 1490 1495 1500 Pro Gln Ser Gly Val Leu Thr Tyr Arg Met Glu Val Thr Glu Ile Gly 1505 1510 1515 1520 Phe Ser Pro Arg Pro Tyr Ala Lys Ala Asn Ile Asp Ile Leu Leu Asn 1525 1530 1535 Gly Lys Ala Val Val Asp Phe Gln Asn Leu Gly Val Met Ile Lys Glu 1540 1545 1550 Glu Asp Glu Cys Thr Arg Tyr Pro Leu Leu Thr Glu Ser Thr Thr Ala 1555 1560 1565 Ser Thr Ala Gln Val Asn Ala Gln Thr Ser Ala Lys Lys Val Tyr Lys 1570 1575 1580 Pro Ala Ser Val Asn Ala Pro Leu Met Ala Gln Ile Pro Asp Leu Thr 1585 1590 1595 1600 Lys Glu Pro Asn Lys Gly Val Ile Pro Ile Ser His Val Glu Ala Pro 1605 1610 1615 Ile Thr Pro Asp Tyr Pro Asn Arg Val Pro Asp Thr Val Pro Phe Thr 1620 1625 1630 Pro Tyr His Met Phe Glu Phe Ala Thr Gly Asn Il e Glu Asn Cys Phe 1635 1640 1645 Gly Pro Glu Phe Ser Ile Tyr Arg Gly Met Ile Pro Pro Arg Thr Pro 1650 1655 1660 Cys Gly Asp Leu Gln Val Thr Thr Arg Val Ile Glu Val Asn Gly Lys 1665 1670 1675 1680 Arg Gly Asp Phe Lys Lys Pro Ser Ser Cys Ile Ala Glu Tyr Glu Val 1685 1690 1695 Pro Ala Asp Ala Trp Tyr Phe Asp Lys Asn Ser His Gly Ala Val Met 1700 1705 1710 Pro Tyr Ser Ile Leu Met Glu Ile Ser Leu Gln Pro Asn Gly Phe Ile 1715 1720 1725 Ser Gly Tyr Met Gly Thr Thr Leu Gly Phe Pro Gly Leu Glu Leu Phe 1730 1735 1740 Phe Arg Asn Leu Asp Gly Ser Gly Glu Leu Leu Arg Glu Val Asp Leu 1745 1750 1755 1760 Arg Gly Lys Thr Ile Arg Asn Asp Ser Arg Leu Leu Ser Thr Val Met 1765 1770 1775 Ala Gly Thr Asn Ile Ile Gln Ser Phe Ser Phe Glu Leu Ser Thr Asp 1780 1785 1790 Gly Glu Pro Phe Tyr Arg Gly Thr Ala Val Phe Gly Tyr Phe Lys Gly 1795 1800 1805 Asp Ala Leu Lys Asp Gln Leu Gly Leu Asp Asn Gly Lys Val Thr Gln 1810 1815 1820 Pro Trp His Val Ala Asn Gly Val Ala Ala Ser Thr Lys Val Asn Leu 1825 1830 1835 1840 Leu Asp Lys Ser Cys Arg His Phe Asn Ala Pro Ala Asn Gln Pro His 1845 1850 1855 Tyr Arg Leu Ala Gly Gly Gln Leu Asn Phe Ile Asp Ser Val Glu Ile 1860 1865 1870 Val Asp Asn Gly Gly Thr Glu Gly Leu Gly Tyr Leu Tyr Ala Glu Arg 1875 1880 1885 Thr Ile Asp Pro Ser Asp Trp Phe Phe Gln Phe His Phe His Gln Asp 1890 1895 1900 Pro Val Met Pro Gly Ser Leu Gly Val Glu Ala Ile Ile Glu Thr Met 1905 1910 1915 1920 Gln Ala Tyr Ala Ile Ser Lys Asp Leu Gly Ala Asp Phe Lys Asn Pro 1925 1930 1935 Lys Phe Gly Gln Ile Leu Ser Asn Ile Lys Trp Lys Tyr Arg Gly Gln 1940 1945 1950 Ile Asn Pro Leu Asn Lys Gln Met Ser Met Asp Val Ser Ile Thr Ser 1955 1960 1965 Ile Lys Asp Glu Asp Gly Lys Lys Val Ile Thr Gly Asn Ala Ser Leu 1970 1975 1980 Ser Lys Asp Gly Leu Arg Ile Tyr Glu Val Phe Asp Ile Ala Ile Ser 1985 1990 1995 2000 Ile Glu Glu Ser Val 2005 <210> 12 <211> 1626 <212> DNA <213> Shewanella putrefaciens SCRC-2874 (FERM BP-1625) <400> 1 atg aat cct aca gca act aac gaa atg ctt tct ccg tgg cca tgg gct 48 Met Asn Pro Thr Ala Thr Asn Glu Met Leu Ser Pro Trp Pro Trp Ala 1 5 10 15 gtg aca gag tca aat atc agt ttt gac gtg caa gtg atg gaa caa caa 96 Val Thr Glu Ser Asn Ile Ser Phe Asp Val Gln Val Met Glu Gln Gln 20 25 30 ctt aaa gat ttt agc cgg gca tgt tac gtg gtc aat cat gcc gac cac 144 Leu Lys Asp Phe Ser Arg Ala Cys Tyr Val Val Asn His Ala Asp His 35 40 45 ggc ttt ggt att gcg caa act gcc gat atc gtg act gaa caa gcg gca 192 Gly Phe Gly Ile Ala Gln Thr Ala Asp Ile Val Thr Glu Gln Ala Ala 50 55 60 aac agc aca gat tta cct gtt agt gct ttt act cct gca tta ggt acc 240 Asn Ser Thr Asp Leu Pro Val Ser Ala Phe Thr Pro Ala Leu Gly Thr 65 70 75 80 gaa agc cta ggc gac aat aat ttc cgc cgc gtt cac ggc gtt aaa tac 288 Glu Ser Leu Gly Asp Asn Asn Phe Arg Arg Val His Gly Val Lys Tyr 85 90 95 gct tat tac gca ggc gct atg gca aac ggt att tca tct gaa gag cta 336 Ala Tyr Tyr Ala Gly Ala Met Ala Asn Gly Ile Ser Ser Glu Glu Leu 100 105 110 ggt att gcc cta ggt caa gct ggc att ttg tgt tcg ttt gga gca gcc 384 Val Ile Ala Leu Gly Gln Ala Gly Ile Leu Cys Ser Phe Gly Ala Ala 115 120 125 ggt ctt att cca agt cgc gtt gaa gcg gcat atac caa gca 432 Gly Leu Ile Pro Ser Arg Val Glu Ala Ala Ile Asn Arg Ile Gln Ala 130 135 140 gcg ctg cca aat ggc cct tat atg ttt aac ctt atc cat agt cct agc 480 Ala Leu Pro Asn Gly Pro Tyr Met Phe Asn Leu Ile His Ser Pro Ser 145 150 155 160 gag cca gca tta gag cgt ggc agc gta gag cta ttt tta aag cat aag 528 Glu Pro Ala Leu Glu Arg Gly Ser Val Glu Leu Phe Leu Lys His Lys 165 170 175 gta cgc acc gtt gaa gca tca gct ttc tta ggt cta aca cca caa atc 576 Val Arg Thr Val Glu Ala Ser Ala Phe Leu Gly Leu Thr Pro Gln Ile 180 185 190 gtc tat tac cgt gca gca gga ttg agc cga gac gca caa ggt aaa gtt 624 Val Tyr Tyr Arg Ala Ala Gly Leu Ser Arg Asp Ala Gln Gly Lys Val 195 200 205 gtg gtt ggt aac aag gtt atc gct aaa gta agt cgc acc gaa gtg gct 672 Val Val Gly Asn Lys Val Ile Ala Lys Val Ser Arg Thr Glu Val Ala210 215 220 gaa aag ttt atg atg cca gcg ccc gca aaa atg cta caa aaa cta gtt 720 Glu Lys Phe Met Met Pro Ala Pro Ala Lys Met Leu Gln Lys Leu Val 225 230 235 240 gat gac ggt tca att acc gct gag caa atg gag ctg gcg caa ctt gta 768 Asp Asp Gly Ser Ile Thr Ala Glu Gln Met Glu Leu Ala Gln Leu Val 245 250 255 cct atg gct gac gac atc act gca gag gcc gat tca ggt ggc cat act 816 Pro Met Ala Asp Asp Ile Thr Ala Glu Ala Asp Ser Gly Gly His Thr 260 265 270 gat aac cgt cca tta gta aca ttg ctg cca acc att tta gcg ctg aaa 864 Asp Asn Arg Pro Leu Val Thr Leu Leu Pro Thr Ile Leu Ala Leu Lys 275 280 285 285 gaa gaa att caa gct aaa tac caa tac gac act cct att cgt gtc ggt 912 Glu Glu Ile Gln Ala Lys Tyr Gln Tyr Asp Thr Pro Ile Arg Val Gly 290 295 300 tgt ggt ggc ggt gtg ggt acg cct gat gca gcg ctg gca ac ttt 960 Cys Gly Gly Gly Val Gly Thr Pro Asp Ala Ala Leu Ala Thr Phe Asn 305 310 315 320 atg ggc gcg gcg tat att gtt acc ggc tct atc aac caa gct tgt gtt 1008 Met Gly Ala Ala Tyr Ile Val Thr Gly Ser Ile As n Gln Ala Cys Val 325 330 335 gaa gcg ggc gca agt gat cac act cgt aaa tta ctt gcc acc act gaa 1056 Glu Ala Gly Ala Ser Asp His Thr Arg Lys Leu Leu Ala Thr Thr Glu 340 345 350 atg gcc gat gtg act atg gca cca gct gca gat atg ttc gag atg ggc 1104 Met Ala Asp Val Thr Met Ala Pro Ala Ala Asp Met Phe Glu Met Gly 355 360 365 gta aaa ctg cag gtg gtt aag cgc ggc acg cta ttc cca atg cgc Lec Val 1152 Gln Val Val Lys Arg Gly Thr Leu Phe Pro Met Arg Ala 370 375 380 aac aag cta tat gag atc tac acc cgt tac gat tca atc gaa gcg atc 1200 Asn Lys Leu Tyr Glu Ile Tyr Thr Arg Tyr Asp Ser Ile Glu Ala Ile 385 390 395 400 cca tta gac gag cgt gaa aag ctt gag aaa caa gta ttc cgc tca agc 1248 Pro Leu Asp Glu Arg Glu Lys Leu Glu Lys Gln Val Phe Arg Ser Ser 405 410 415 cta gat gaa ata tgg gca ggt aca gtg g ttt aac gag cgc gac 1296 Leu Asp Glu Ile Trp Ala Gly Thr Val Ala His Phe Asn Glu Arg Asp 420 425 430 cct aag caa atc gaa cgc gca gag ggt aac cct aag cgt aaa atg gca 1344 Pro Lys Gln Ile Glu Arg Ala Glu Gly Asn Pro Lys Arg Lys Met Ala 435 440 445 ttg att ttc cgt tgg tac tta ggt ctt tct agt cgc tgg tca aac tca 1392 Leu Ile Phe Arg Trp Tyr Leu Gly Leu Ser Ser Arg Trp Ser Asn Ser 450 455 460 ggc ga gtk ggt cgt gaa atg gat tat caa att tgg gct ggc cct gct 1440 Gly Glu Val Gly Arg Glu Met Asp Tyr Gln Ile Trp Ala Gly Pro Ala 465 470 470 475 480 ctc ggt gca ttt aac caa tgg gca aaa ggc agt tac tat 1488 Leu Gly Ala Phe Asn Gln Trp Ala Lys Gly Ser Tyr Leu Asp Asn Tyr 485 490 495 caa gac cga aat gcc gtc gat ttg gca aag cac tta atg tac ggc gcg 1536 Gln Asp Arg Asn Ala Val Asp Leu Ala Lys Met Tyr Gly Ala 500 505 510 gct tac tta aat cgt att aac tcg cta acg gct caa ggc gtt aaa gtg 1584 Ala Tyr Leu Asn Arg Ile Asn Ser Leu Thr Ala Gln Gly Val Lys Val 515 520 525 525 cca gca cag tta ctt cg aag cca aac caa aga atg gcc 1626 Pro Ala Gln Leu Leu Arg Trp Lys Pro Asn Gln Arg Met Ala 530 535 540 <210> 13 <211> 542 <212> PRT <400> 1 Met Asn Pro Thr Ala Thr Asn Glu Met Leu Ser Pro Trp Pro Trp Ala 1 5 10 15 Val Thr Glu Ser Asn Ile Ser Phe Asp Val Gln Val Met Glu Gln Gln 20 25 30 Leu Lys Asp Phe Ser Arg Ala Cys Tyr Val Val Asn His Ala Asp His 35 40 45 Gly Phe Gly Ile Ala Gln Thr Ala Asp Ile Val Thr Glu Gln Ala Ala 50 55 60 Asn Ser Thr Asp Leu Pro Val Ser Ala Phe Thr Pro Ala Leu Gly Thr 65 70 75 80 Glu Ser Leu Gly Asp Asn Asn Phe Arg Arg Val His Gly Val Lys Tyr 85 90 95 Ala Tyr Tyr Ala Gly Ala Met Ala Asn Gly Ile Ser Ser Glu Glu Leu 100 105 110 Val Ile Ala Leu Gly Gln Ala Gly Ile Leu Cys Ser Phe Gly Ala Ala 115 120 125 Gly Leu Ile Pro Ser Arg Val Glu Ala Ala Ile Asn Arg Ile Gln Ala 130 135 140 Ala Leu Pro Asn Gly Pro Tyr Met Phe Asn Leu Ile His Ser Pro Ser 145 150 155 160 Glu Pro Ala Leu Glu Arg Gly Ser Val Glu Leu Phe Leu Lys His Lys 165 170 175 Val Arg Thr Val Glu Ala Ser Ala Phe Leu Gly Leu Thr Pro Gln Ile 180 185 190 Val Tyr Tyr Arg Ala Ala Gly Leu Ser Arg Asp Ala Gln Gly Lys Val 195 200 205 Val Val Gly As n Lys Val Ile Ala Lys Val Ser Arg Thr Glu Val Ala 210 215 220 Glu Lys Phe Met Met Pro Ala Pro Ala Lys Met Leu Gln Lys Leu Val 225 230 235 240 Asp Asp Gly Ser Ile Thr Ala Glu Gln Met Glu Leu Ala Gln Leu Val 245 250 255 Pro Met Ala Asp Asp Ile Thr Ala Glu Ala Asp Ser Gly Gly His Thr 260 265 270 Asp Asn Arg Pro Leu Val Thr Leu Leu Pro Thr Ile Leu Ala Leu Lys 275 280 285 Glu Glu Ile Gln Ala Lys Tyr Gln Tyr Asp Thr Pro Ile Arg Val Gly 290 295 300 Cys Gly Gly Gly Val Gly Thr Pro Asp Ala Ala Leu Ala Thr Phe Asn 305 310 315 320 Met Gly Ala Ala Tyr Ile Val Thr Gly Ser Ile Asn Gln Ala Cys Val 325 330 335 Glu Ala Gly Ala Ser Asp His Thr Arg Lys Leu Leu Ala Thr Thr Glu 340 345 350 Met Ala Asp Val Thr Met Ala Pro Ala Ala Asp Met Phe Glu Met Gly 355 360 365 Val Lys Leu Gln Val Val Lys Arg Gly Thr Leu Phe Pro Met Arg Ala 370 375 380 Asn Lys Leu Tyr Glu Ile Tyr Thr Arg Tyr Asp Ser Ile Glu Ala Ile 385 390 395 400 Pro Leu Asp Glu Arg Glu Lys Leu Glu Lys Gln Val Phe Arg Ser Ser 405 410 415 Leu Asp Glu I le Trp Ala Gly Thr Val Ala His Phe Asn Glu Arg Asp 420 425 430 Pro Lys Gln Ile Glu Arg Ala Glu Gly Asn Pro Lys Arg Lys Met Ala 435 440 445 Leu Ile Phe Arg Trp Tyr Leu Gly Leu Ser Ser Arg Trp Ser Asn Ser 450 455 460 Gly Glu Val Gly Arg Glu Met Asp Tyr Gln Ile Trp Ala Gly Pro Ala 465 470 475 480 Leu Gly Ala Phe Asn Gln Trp Ala Lys Gly Ser Tyr Leu Asp Asn Tyr 485 490 495 Gln Asp Arg Asn Ala Val Asp Leu Ala Lys His Leu Met Tyr Gly Ala 500 505 510 Ala Tyr Leu Asn Arg Ile Asn Ser Leu Thr Ala Gln Gly Val Lys Val 515 520 520 Pro Ala Gln Leu Leu Arg Trp Lys Pro Asn Gln Arg Met Ala 530 535 540

[Brief description of the drawings]

FIG. 1 shows pJRD which is an embodiment of the plasmid of the present invention.
1 shows the structure of EPA-S.

[Procedure amendment]

[Submission date] August 29, 2000 (2008.2.
9)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0013

[Correction method] Change

[Correction contents]

Example 1 Preparation of miniaturized plasmid EP inserted into plasmid pEPA described in JP-A-8-242867
ORF3, which is essential for EPA biosynthesis,
6, 7, 8 and 9 were subcloned. ORF5,
For 6, 7, 8 and 9, the cloning vector pBSI
XbaI-SpeI at the XbaI-SpeI site of IKS (+) (Stratagene)
Fragment (23,045-31,443), XbaI-XbaI fragment (1
2,314-23,045) SpeI-NheI fragment (31,443-32,5)
14) was sequentially subcloned to prepare ΔX4XbNh / pBS. ΔX4XbNh / pBS treated with NotI was blunt-ended with T4 DNA polymerase and treated with XhoI to obtain DNA fragment A. For ORF3, R / pSTV28
(HpaI fragment 7,951-9,129 was converted to Sma of Takara Shuzo vector pSTV28.
The fragment cut out after treating with EcoRI and PstI) was inserted into the EcoRI-PstI site of pBSIIKS (+) to
pBS was prepared. After treating R / pBS with PstI, blunt ends were created with T4 DNA polymerase and an XhoI linker was introduced, and then Xh
A fragment containing ORF3 was cut out with oI to obtain a DNA fragment B. Fragment A is introduced into the XhoI-StuI site of pJRD215 (kanamycin and streptomycin resistance), which is a broad-range host vector, using the Pacazine Lambda DNA packaging system (Promega), and fragment B is inserted into the XhoI site using a DNA ligation kit (Takara Shuzo). ) To complete the plasmid. This was named pJRDEPA-S (Figure 1).

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0025

[Correction method] Change

[Correction contents]

[0025]

[Sequence List] <110> Sagami Chemical Reserach Center, Japan Bioindustry Association, Director-General of Agency of Industrial Science and Technology. <120> A plasmid carrying the eicosapenta e noic acid synthesis gene claste r and a transgenic cyanobacterium producing eicosapenta e noic acid. <130> SO18226 <160> 13

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) (C12N 1/12 (C12P 7/64 C12R 1:89) C12R 1:89) (C12P 7/64 C12N 15 / 00 ZNAA C12R 1:89) C12R 1:89) (72) Inventor Reiko Yu 3-24-4-108, Kirigaoka, Midori-ku, Yokohama-shi, Kanagawa Prefecture (72) Inventor Akiko Yamada 6-1-, Wakamatsu, Sagamihara-shi, Kanagawa Prefecture 27-B-305 (72) Inventor, Makoto Matsunaga 4-20-15, Honmachi, Koganei-shi, Tokyo (72) Inventor Haruko Takeyama, 2-40-C-106, Sachimachi, Fuchu-shi, Tokyo (72) Inventor, Ryuichiro Kurane 1-3-1-3 Higashi, Tsukuba City, Ibaraki Prefecture F-term (Reference) 4B024 AA03 BA07 CA04 DA06 GA14 HA01 4B064 AD90 CA02 CA08 CA19 CC24 DA16 4B065 AA01X AA01Y AA26X AB01 BA02 BA22 CA 13

Claims (3)

[Claims] 1. SEQ ID NOs: 2, 4, 6, 8, 10, and 1
A plasmid obtained by cloning a gene group encoding a group of eicosapentaenoic acid biosynthetic enzymes encoded by the nucleotide sequence represented by 2 into a broad-range host vector. 2. SEQ ID NOs: 3, 5, 7, 9, 11, and 1
A plasmid obtained by cloning a gene group encoding the icosapentaenoic acid biosynthetic enzyme group represented by the nucleotide sequence represented by 3 into a broad-range host vector. 3. A cyanobacterium producing icosapentaenoic acid obtained by introducing the plasmid according to claim 1 or 2.