JP2000239179A - Improving exercise performance by suppressing ammonia production during exercise - Google Patents

Abstract

(57) [Summary] [Problem] Ingestion of high protein by strengthening muscles causes intestinal putrefaction products to accumulate in the blood, kidneys deteriorate (excretion / purification of blood), and those waste products are passed through the blood to the body. Circulate. As a result, muscle strengthening cannot be continued.
Also, due to the production of ammonia from the brain and muscles during exercise,
If the blood ammonia level rises extremely and accumulates, it will cause fatigue and cause muscle cramps. The challenge is to use products based on champignon extract to maintain and improve athletic performance of athletes. SOLUTION: For the purpose of improving the intestinal environment of athletes and suppressing the production of ammonia in the body, commercialization of a compound containing a champignon extract has been realized, which has a great effect on maintaining and improving exercise performance. Commercialization is due to oral ingestion, 300
In the size of mg, it was formed into a triangular shape. In addition, it was designed to sufficiently satisfy the daily intake.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a method for treating a patient suffering from illness in the medical field by using a champignon extract which has an effect of suppressing the production of ammonia, for central fatigue and muscle fatigue caused by the production of ammonia in the body during exercise. The goal is to prevent athletes and maximize the athletic ability of athletes.

[0002]

2. Description of the Related Art At present, a method for suppressing the production of ammonia during exercise is not commercially available as a product by oral ingestion. Carbo-loading techniques over long distances, such as marathons, may be the way to suppress the production of ammonia during exercise. According to the competition schedule, 4
Ingest only carbohydrates and no protein for a long time. This is a method in which glycogen is sufficiently stored in muscles and the day of the tournament is approached, and is also called glycogen loading. Since ammonia is generated by the decomposition of proteins (amino acids), this method has been adopted. However, it is considered to be a method for suppressing the generation of ammonia from brain and muscle during exercise as much as possible. This method is implemented according to each player because the effect is not constant due to different physiological functions of each person. It is thought that if the ammonia generated from the brain and muscle during exercise can be uniformly suppressed by a simple method, the exercise ability will be improved.

[0003]

The source of amino acids in living organisms is endogenous proteins that form tissues with proteins in food. Each protein is decomposed into amino acids and then reused in the body for protein synthesis, or further decomposed and converted into glucose (glucose) or fat, or completely oxidized to carbon dioxide and water. In the degradation of amino acids, their carbon skeleton and amino groups are processed by alternative metabolic pathways. That is, the carbon skeleton is converted to another substance or completely oxidized, but the amino group undergoes a rearrangement reaction to finally produce ammonia. Ammonia is further converted to urea in the liver and excreted in urine.
A person's blood ammonia concentration is 30-40 μm at rest.
mol / l, but the production in the muscle during exercise depends on the degree of increase and the exercise conditions.
It increases to more than μmol / l. The origin of this ammonia is also generated by amino acid degradation, but mainly in the purine nucleotide cycle, inosine 5′-phosphate (IMP) is generated from adenosine 5′-phosphate (AMP) by AMP deaminase. Ammonia that is released when It is believed that the accumulation of ammonia promotes fatigue for the following reasons. When ammonia accumulates in the brain by the action of ammonia on the brain and nervous system, it produces glutamine and decreases glutamate. Glutamate is required for the production of γ-aminobutyric acid (GABA), a regulator of the activity of nerve cells, causing its reduction, resulting in nervous system dysfunction and central fatigue. When ammonia accumulates in the muscle due to the action on muscle, glycolytic phosphofructokinase is activated to promote glycolysis and promote the production of lactic acid. As a result, the muscle is acidified. Further, ammonia binds to α-ketoglutaric acid, which is a component of the citric acid cycle, to become glutamic acid, thereby inhibiting the rotation of the citric acid cycle. For these reasons, ammonia also promotes muscle fatigue. In muscle building, high protein intake is indispensable, but it is meaningless if you consume enough carbohydrates before taking protein. Many high-protein diets are commercially available because muscle mass has an absolute effect on competitive performance, but the burden on the kidneys, such as the processing of ammonia generated from large amounts of amino acids, has become a serious problem. I have. From the above, exercise ability can be improved by suppressing the production of ammonia in the body.

[0004]

[MEANS FOR SOLVING THE PROBLEMS] By realizing a product that can exhibit the effect of suppressing the production of ammonia in the body containing a champignon extract as a main component, the product is used to maintain and improve the athletic ability of athletes during exercise. is there. For commercialization, each tablet was formed into a triangular shape with 300 mg, a champignon extract as a main component, and calculated to satisfy the daily intake, thereby realizing the product.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION A champignon extract can maintain its material as natural and natural as possible and maintain its freshness. -Consideration must be given to a product form that has few quality deterioration factors such as air oxidation. In addition to this, factors such as daily intake, absorption in the body, ease of eating, ease of handling, cost, and related laws and regulations must also be considered. The product by the champignon extract is a food that improves the intestinal environment and suppresses the ammonia generated from brain and muscle by combining it with a known food or food ingredients, excipients, and pharmaceutical carriers in a manner known per se. be able to. The food or food component to be used is not particularly limited, and can be appropriately selected by those skilled in the art according to the intended use of improving the intestinal environment and suppressing the production of ammonia. Also, the form is not particularly limited, and various forms and shapes can be used according to specific applications.

[0006]

EXAMPLES The effect of inhibiting the putrefaction products of champignon extract on humans is described in a clinical trial report on the use of a product containing a champignon extract, but the effect of inhibiting blood ammonia is as follows. 1 14 elderly inpatients were given champignon extract (BX
The average value of the blood ammonia concentration when 1 g of (50FPD) was administered for 30 days decreased from 93 μg / dl before administration to 45 μg / dl at the end of administration, a 52% decrease. Twenty nine ostomy patients were treated with Champignon Extract (BX)
The average value of the blood ammonia concentration when a product containing 1 g of 50 FPD) per packet was administered for one month was significantly (p (0.01) from 46 μg / dl before administration to 28 μg / dl at the end of administration. 3) Liver cirrhosis progressed to hyperammonemia, 61 hospitalized
-Year-old woman, champignon extract (BX50F
(PD) The blood ammonia concentration when the combination product was administered twice a day decreased from 182 μg / dl before administration to 82 μg / dl by 55% at the end of administration.

For dogs, Illinois State University, USA
In a dog fecal odor control test performed using two beagle dogs, administration of a champignon extract (150 mg /
Head) Sensory test before and after significantly showed fecal odor (p (0.01)
Although the blood urea nitrogen (BUN) measured at the same time was significantly reduced by 41 to 48%, the BUN measurement value before administration was significantly (P (0.01) reduced by 27 to 38%). An inhibitory effect of a certain intestinal putrefaction product was confirmed.

In rabbits, indole and tryptamine are intestinal degradation products of animal proteins, and are known to cause malignant tumors and leukemia in lymphoid tissues by oral administration. An experiment was performed using rabbits to measure the blood levels of indoleacetic acid and tryptamine produced by intestinal putrefaction of proteins and to confirm the effect of administration of champignon extract on the control of putrefactive products in blood. In this experiment, 21 healthy rabbits were selected and divided into three groups. The first group (5 animals) was used as a control group. A mixed solution of 1.0 g / kg of tryptophan and 10 ml of water was orally administered to 5 animals of the second group using a catheter. 1.0 g / kg of tryptophan was mixed with 10 ml of water in 11 animals of the third group, and champignon extract (BX100FPD 0.25 / kg) was further added.
Was added orally by a catheter. Each group had free water feeding. At 1, 3, 6, 9, and 12 hours after administration, 1 ml of blood was collected from rabbits of each group from the ear vein, and immediately centrifuged at 3000 rpm for 10 minutes to separate serum. Then, 25% (W / V) of the same volume as serum
After adding trichloroacetic acid and leaving it to stand for 10 minutes,
The mixture was centrifuged at 0 rpm for 10 minutes to remove proteins.
The supernatant was filtered through a 0.45 μm filter (DISMIC) to obtain a sample for HPLC measurement. The results showed that when tryptophan was orally administered to rabbits, indoleacetic acid and tryptamine were detected in the blood, but no tryptamine was detected in rabbits co-administered with champignon extract, and indoleacetic acid was also detected 12 hours after administration. lost.
Thus, it was confirmed that the champignon extract inhibits absorption of blood by suppressing the production of tryptamine and indoleacetic acid due to intestinal putrefaction of meat components.

For chickens, the results of the measured blood ammonia levels as part of the stool odor control test performed by the Faculty of Agriculture at Tottori University also suggest the effect of champignon extract on the intestinal putrefaction products. are doing. In the feed champignon extract administration group (10 ppm in feed for 3 weeks), the average blood ammonia concentration was reduced by 35% compared to the control group,
This reduction was statistically significant at the 95% level.

[0010]

The origin of amino acids in living organisms is endogenous proteins that form tissues with proteins in food.
Either protein is broken down into amino acids and then reused in the body for protein synthesis, or further broken down into glucose (glucose) or fat,
Completely oxidized to carbon dioxide and water. In the degradation of amino acids, their carbon skeleton and amino groups are processed by alternative metabolic pathways. That is, the carbon skeleton is converted to another substance or completely oxidized, but the amino group undergoes a rearrangement reaction to finally produce ammonia. Ammonia is further converted to urea in the liver and excreted in urine. The blood ammonia concentration of a human at rest is 30 to 40 μmol / l,
The production in the muscle during exercise increases to 100 μmol / l or more, depending on the degree of increase and the exercise conditions. The increase in blood ammonia concentration during exercise increases the amount of ammonia produced by the breakdown of amino acids and the brain and muscles, causing it to circulate through the blood, placing a heavy burden on the kidneys and liver, May cause reduced exercise capacity. Suppressing the production of ammonia can maintain healthy renal and hepatic functions, improve the intestinal environment of athletes, create necessary conditions during exercise, and maintain and improve exercise performance.

Claims (1)

[Claims] Claims 1. An improvement in exercise ability by ingesting a functional food, which is obtained from a champignon extract and is characterized by inhibiting the production of ammonia.