CN224100738U - Microfluidic chip - Google Patents

Abstract

The utility model provides a microfluidic chip, relates to the technical field of microfluidic detection, and solves the technical problem that a centrifugal microfluidic chip in the prior art cannot directly observe whether the sample amount in a sample adding groove is proper or not. The device comprises a chip substrate and a sealing membrane packaged on the upper surface of the substrate, wherein a flat long groove is formed along the upper surface of the substrate and forms a sample adding groove with the sealing membrane, a sample adding port is formed in the sealing membrane and communicated with the sample adding groove and used for injecting samples into the sample adding groove, and a supporting device is arranged and used for supporting the sealing membrane on the sample adding groove. According to the utility model, by designing the sample adding groove structure, an operator can intuitively observe whether the sample adding process and the sample adding quantity are proper or not.

Description

Microfluidic chip

Technical Field

The utility model relates to the technical field of microfluidic detection, in particular to a microfluidic chip.

Background

Microfluidic (Microfluidics) chip technology has taken a very important role in the biomedical field, in particular in Nucleic acid detection (Nucleic ACID TESTING, NAT), by virtue of its unique advantages of miniaturization and high integration. The essence of the technology is that complex links such as sample pretreatment, mixing, chemical reaction, separation and detection can be integrated on one or more chips with tiny volumes, so that a micro laboratory can be created. The microfluidic chip greatly reduces the consumption requirements of samples and reagents, simplifies the operation flow, greatly shortens the detection period, and simultaneously can effectively avoid various errors possibly occurring in the manual operation process of the traditional laboratory. As such, microfluidic technology is the technology of choice in many fields such as chemical analysis, DNA sequencing, protein analysis, single cell and single molecule analysis, food safety monitoring, environmental monitoring, and drug screening. With the continuous deep research and the continuous development of technology, the application range of the microfluidic chip is expanding, and the microfluidic chip has great potential and is regarded as a key technology for possibly generating great changes to the life style of human beings in the future.

The planar structure diagram of the current microfluidic chip is shown in fig. 1, and is divided into an upper layer and a lower layer, wherein the upper layer is connected with the lower layer in a watertight manner, a group of sample adding through holes for sample adding are formed in the upper layer of the chip, and sample adding grooves for containing samples injected into the through holes are formed in the lower layer of the chip at positions corresponding to the sample adding through holes. During centrifugal operation, the liquid sample enters the detection holes through various functional grooves arranged on the lower layer and microfluidic channels with different shapes under the action of centrifugal force, and is subjected to optical detection. In the process, the sample needs to pass through a longer channel and multiple separations, and only a sufficient amount of sample is injected to ensure that the sample amount finally entering the detection hole is enough to carry out effective detection, so that the depth of the existing sample adding groove is as large as possible, and the capacity of the existing sample adding groove is generally larger than the sample amount required by one-time detection. In order to prevent sample overflow, the sample adding groove volume of the existing chip design is far larger than the required sample volume, and samples fall at the bottom of the sample adding groove after sample adding, so that whether the sample adding amount is proper cannot be intuitively observed. And too much or too little sample size can affect the functions of the microfluidic chip, thereby affecting the detection result. In view of the above, there is an urgent need to develop a novel microfluidic chip for solving the above-mentioned technical problems.

Disclosure of utility model

The utility model aims to provide a micro-fluidic chip to solve the related technical problems in the prior art. The preferred technical solutions of the technical solutions provided by the present utility model can produce a plurality of technical effects described below.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

The microfluidic chip comprises a chip substrate, a sealing membrane, a plurality of detection holes, a microfluidic channel and a sealing membrane, wherein a sample adding groove is formed in the chip substrate and used for temporarily storing samples, the sealing membrane is covered on the chip substrate, a sample adding port is formed in the sealing membrane and communicated with the sample adding groove and used for injecting samples into the sample adding groove, the detection holes are uniformly distributed in the circumferential direction of the chip substrate and used for containing samples to be detected, the microfluidic channel is used for communicating the sample adding groove with the detection holes, the sample adding groove is formed in an extending mode along the upper surface of the chip substrate and is in a prolate groove shape, and the substrate and the sealing membrane form the sample adding groove.

According to one embodiment of the utility model, the support assembly is arranged and comprises a support ring which is arranged on the chip substrate and used for supporting the sealing membrane, the outer circumferential side wall of the support ring is fixedly connected to the side wall of the sample adding groove, the center of the support ring is provided with a through cavity in a penetrating way, the sample adding port is communicated with the upper end of the through cavity, and the lower end of the through cavity is communicated with the sample adding groove.

According to another embodiment of the utility model, a supporting component is arranged, the supporting component comprises a supporting ring which is arranged on the chip substrate and used for supporting the sealing membrane, one end of the supporting ring is abutted against the bottom of the sample adding groove and fixedly connected with the chip substrate, the other end of the supporting ring is abutted against the sealing membrane, a through cavity is arranged in the center of the supporting ring in a penetrating manner, a notch communicated with the through cavity is arranged at the bottom end of the supporting ring in the direction perpendicular to the through cavity, the sample adding port is communicated with the upper end of the through cavity, and the sample adding groove is communicated with the through cavity through the notch.

According to another embodiment of the utility model, a support assembly is provided, the support assembly comprises a support ring which is arranged on the chip substrate and used for supporting the sealing membrane, one end of the support ring is abutted against the bottom of the sample adding groove and fixedly connected with the chip substrate, the other end of the support ring is abutted against the sealing membrane, the support ring is arranged in a semicircular ring shape, a through cavity is formed in the center of the support ring in a penetrating mode, a notch of the through cavity is formed in the circumferential side face of the support ring in the penetrating direction of the through cavity, the sample adding port is communicated with the upper end of the through cavity, and the sample adding groove is communicated with the through cavity through the notch.

Further, the notch expands radially outwardly, i.e., the distance between the inner port of the notch is less than the distance between the outer port of the notch.

Preferably, the top surface of the support ring is fixedly connected with the sealing membrane.

According to another embodiment of the present utility model, the loading slot is provided to extend in an arc shape starting from the loading port, and the loading slot is provided along the circumferential direction of the chip substrate.

Further, the support assembly further comprises a plurality of support sheets which are uniformly distributed along the extending direction of the sample adding groove, one end of each support sheet is fixedly connected to the chip substrate, and the other end of each support sheet is fixedly connected to the sealing membrane.

Further, the support assembly further comprises a support piece arranged along the extending direction of the sample adding groove, and the support piece is arranged in an arc shape and is positioned in the middle of the sample adding groove.

According to a further embodiment of the utility model, the sealing membrane is provided with a dosing line for indicating the amount of sample added.

The following main technical effects of the utility model are:

According to the utility model, the sample adding port and the arc-shaped sample adding groove of the flat groove are arranged on the sealing diaphragm, so that an operator can intuitively observe whether the sample adding process and the sample adding amount are proper, and therefore, a proper amount of samples can be injected in each detection, and the success rate of detection and the accuracy of results are improved.

According to the utility model, the sealing membrane is supported by introducing the supporting component, particularly below the sample adding port, so that the deformation or collapse of the sealing membrane is effectively prevented, and the problem of insufficient sample adding caused by deformation of the membrane is avoided.

In summary, through innovative improvement of the prior art, the utility model solves the technical problem that the existing microfluidic chip is easy to appear in the sample adding process, provides a new solution for the development of the microfluidic chip technology, and is expected to greatly improve the efficiency and the result accuracy of related item detection in the field of biological detection.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic plan view of a sample addition structure of a centrifugal microfluidic chip according to the prior art;

FIG. 2 is a schematic view of the overall structure provided in embodiment 1 of the present utility model;

fig. 3 is a schematic diagram of an internal structure provided in embodiment 1 of the present utility model:

FIG. 4 is a schematic view of a part of the structure provided in embodiment 1 of the present utility model;

FIG. 5 is a schematic view of a part of the structure provided in embodiment 2 of the present utility model;

FIG. 6 is a schematic view of a part of the structure provided in embodiment 3 of the present utility model;

FIG. 7 is a schematic view of a part of the structure provided in embodiment 4 of the present utility model;

FIG. 8 is a schematic view of a part of the structure provided in embodiment 5 of the present utility model;

FIG. 9 is a schematic view of a part of the structure provided in embodiment 6 of the present utility model;

FIG. 10 is a schematic view showing the effect of the sample addition tank of the present utility model compared with the prior art after improvement;

The reference numerals indicate 100, a chip substrate, 110, a sealing membrane, 120, a detection hole, 130, a sample adding groove, 140, a microfluidic channel, 150, a sample adding port, 200, a support assembly, 210, a support ring, 220, a through cavity, 230, a notch, 240, a support sheet, 250 and a quantitative line.

Detailed Description

In order to make the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the utility model. All other embodiments, based on the examples herein, which are within the scope of the utility model as defined by the claims, will be within the scope of the utility model as defined by the claims.

In the description of the present utility model, it should be noted that unless otherwise indicated, the terms "plurality of" means two or more, "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", etc. are merely for convenience of description and for simplicity of description, and do not necessarily indicate or imply that the apparatus or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the utility model. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should also be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, integrally connected, mechanically connected, electrically connected, directly connected, or indirectly connected via an intermediary. The specific meaning of the above terms in the present utility model can be understood as appropriate by those of ordinary skill in the art.

The application is further described in detail below with reference to fig. 2-10, and an embodiment of the application discloses a microfluidic chip. Comprises a chip substrate and a sealing membrane encapsulated on the upper layer of the substrate, wherein various functional grooves and micro-flow channels with different shapes are arranged on the substrate, for placing and carrying the required test sample, the chip substrate is preferably designed in the shape of a disk for ease of handling and improved space utilization.

Specifically, as shown in fig. 2-3, the chip substrate 100 is provided with a sample loading slot 130 near the center of the substrate, and a plurality of detection holes 120 formed on the chip substrate 100. The plurality of detection holes 120 are distributed near the outer edge of the chip substrate 100 and are uniformly arranged along the circumferential direction of the chip substrate 100, so that samples (e.g., biological liquid samples such as blood or urine) can be uniformly distributed among detection points, and the consistency and reliability of detection are improved.

The chip substrate 100 is further provided with a microfluidic channel 140 for communicating the sample addition groove 130 with the plurality of detection holes 120. The design of the microfluidic channel 140 not only ensures that the liquid sample can smoothly flow from the sample loading slot 130 to each detection hole 120, but also can flexibly adjust the flow characteristics and residence time of the sample according to different detection requirements by adjusting the design (such as width, depth and path) of the microfluidic channel 140, thereby optimizing the detection effect. In addition, the notch of the sample loading slot 130, the hole end of the detection hole 120 and the opening of the microfluidic channel 140 are all located on the top surface of the chip substrate 100, so that an operator can conveniently load and observe samples.

The sealing membrane 110 is made of transparent materials, is fixed on the upper surface of the substrate 100 in a gluing way, and forms a sample temporary storage area with the sample adding groove 130 on the substrate 100, so that the sealing performance and stability of the microfluidic chip are maintained. The sealing membrane 110 is provided with a sample loading port 150 which is communicated with the sample loading groove 130, and allows an operator to inject a sample to be detected into the sample loading groove 130 through the sample loading port 150. The design ensures the leak-free addition of the sample, simplifies the operation steps and improves the working efficiency.

The sample loading slot 130 is a prolate slot, i.e. a slot with a length and a width extending along the upper surface of the chip substrate 100, a height along the thickness direction of the chip and a width greater than the height, and a section of the sample loading slot is a rectangle or rectangle-like shape with a length far greater than the width. In this way, an oblong sample addition groove 130 is formed between the chip substrate 100 and the sealing membrane 110, after the sample is added into the sample addition groove 130, the sample contacts with the surface of the substrate 100 and the sealing membrane 110, and under the action of capillary force, the sample can flow along the groove wall of the sample addition groove 130 and the surface of the sealing membrane 110 toward the end part of the sample addition groove 130 away from the sample addition port 150 until the whole sample addition groove 130 is filled, so that an operator can intuitively see the added liquid sample amount. By precisely designing the capacity of the loading slot 130, it is ensured that the sample to be loaded is as close as possible to the sample size required for detection.

The sealing membrane 110 may be made of plastic, glass, quartz, or the like. In one embodiment, the sealing membrane 110 may be a plastic film, which has a certain elasticity and can be deformed to a certain extent without being torn under the action of a proper external force. In this embodiment, the size of the loading hole 150 is adapted to the loading tip, and when the loading tip is inserted into the loading hole 150, the loading hole 150 is tightly matched with the outer wall of the loading tip, so as to seal the loading hole 150, seal one end of the loading slot 130, and when the sample is loaded, the sample flows toward the other end of the loading slot 130 under the action of the liquid pressure, so that the sample fills the whole loading slot 130 rapidly.

In addition, in order to fill the sample well 130 more smoothly and prevent the sample from accumulating or even overflowing at the sample inlet 150, in one embodiment, the surface of the substrate 100 and the surface of the sealing membrane 110 on the inner wall of the sample well 130 are subjected to hydrophilic treatment, for example, hydrophilic agent is coated on the surface of the substrate 100 or/and the surface of the sealing membrane 110.

In addition, in order to precisely control the addition amount of the sample, the present application also provides a sample addition quantitative line 250. The quantitative line 250 may be provided at the bottom of the sample addition groove 130, or may be provided on the sealing membrane 110 and arranged along the extending direction of the fan shape. When a sample is added to the quantitative line 250, it is indicated that the added sample volume has been sufficiently filled in all the detection wells 120 without overflowing after passing through the microfluidic channels and functional channels, and the desired amount of liquid required for sample detection is achieved. The design not only simplifies the operation flow and avoids the situation of adding excessive or insufficient samples, but also ensures the consistency and accuracy of each detection and greatly improves the reliability and repeatability of the detection result.

FIG. 10 is a schematic diagram showing the technical effect of the sample addition tank according to the present application compared with the prior art. In the improved structure of FIG. 10B, after the sample is added from the sample adding port, the operator can visually observe the change of the sample adding amount, such as observing whether the sample adding amount reaches the quantitative line position or not, and accurately grasp the sample adding amount.

Referring to fig. 3 and 4, in one embodiment of the present application, the loading slot 130 is designed to extend in an arc shape along the circumferential direction of the chip substrate 100, and the top view thereof shows a fan-shaped distribution. That is, the sample loading slot 130 has a first arc sidewall close to the center of the substrate and a second arc sidewall far away from the center of the substrate, wherein the second arc sidewall gradually gets away from the center of the chip from one end (one end of the sample loading port) of the sample loading slot 130 to the other end, so that the sample loading slot 130 gradually gets away from the center of the substrate from the sample loading port. The unique design not only increases the physical capacity of the sample adding groove 130, so that the sample adding groove 130 can more efficiently contain all samples required in the detection process, but also can enable all samples in the sample adding groove 130 to enter the functional groove and the micro-flow channel in the centrifugation process, thereby reducing sample waste and improving the detection efficiency. In addition, the cross-sectional area of the sample loading slot 130 gradually increases from one end of sample loading to the other end, which is beneficial to the rapid flow of the sample into the sample loading slot, so that the sample is prevented from being accumulated near the sample loading port during sample loading. In addition, the design also makes the operator more easily observe the condition of adding of sample, has strengthened the intuitiveness and the convenience of operation.

It is worth noting that when the upper layer of the microfluidic chip is packaged by a thin film material, the thin film material is easy to deform under the extrusion of a sample adding gun during sample adding, and the capacity of an actual sample adding groove after deformation is smaller than the design capacity, so that insufficient sample amount is easy to cause. In view of the above shortcomings, the following examples illustrate various embodiments of the present application in detail.

Example 1

Referring to fig. 3 and 4, in one embodiment of the present application, a support assembly 200 is further disposed on the chip substrate 100. The supporting assembly 200 includes a supporting ring 210 fixedly mounted on the chip substrate 100, the bottom end of the supporting ring 210 is fixedly connected to the bottom of the sample loading slot 130, the top end of the supporting ring is fixedly connected to the sealing membrane 110, and the supporting ring 210 and the sealing membrane 110 can be connected to each other by gluing, so that the stability of the structure is ensured. And the supporting ring 210 is disposed at one end of the sample loading slot 130 away from the communication point of the microfluidic channel 140, so as to avoid interference to the microfluidic channel 140 during the sample loading process and ensure the unobstructed flow path of the sample.

The center of the supporting ring 210 is provided with a through cavity 220 in a penetrating way, and the top end of the through cavity 220 corresponds to and is communicated with the sample inlet 150 on the sealing membrane 110. Allowing the loading gun or sample receiving tube to smoothly load sample into the through-cavity 220 through the loading port 150 and to guide the sample into the interior of the loading slot 130. The side wall of the support ring 210 is provided with a notch 230 facing the sample loading groove 130 and arranged along the height direction, and the notch 230 is communicated with the through cavity 220, i.e. the support ring 210 is arranged in a semicircular ring. The design not only provides a direct inflow path for the sample, but also plays a role in guiding in the sample adding process, prevents the sample from overflowing or splashing, and ensures the cleanness and safety of the sample adding process.

The notch 230 radially expands outwards, that is, the inner end of the notch 230 is smaller than the outer end distance of the notch 230, that is, four vertexes of the notch 230 are connected with each other to form a trapezoid, so that samples in the through cavity 220 can flow outwards conveniently, that is, the samples can flow out of the sample adding groove 130 directly from the notch 230 after entering the through cavity 220, and aggregation of the samples in the through cavity 220 is avoided.

When the loading gun or the sample receiving tube injects the sample into the loading slot 130 through the loading port 150, the top end of the support ring 210 support ring will realize rigid support for the loading gun or the sample receiving tube, and these devices can be effectively supported, preventing the sealing membrane 110 from deforming or collapsing due to uneven pressure. If the sealing membrane 110 is deformed or collapsed, an unnecessary fit between the loading slot 130 and the sealing membrane 110 may be generated, preventing the normal inflow of the sample, and even causing loading failure. Therefore, the design of the support ring 210 not only ensures smooth operation of the sample loading process, but also significantly improves the safety and reliability of the operation.

In addition, a groove is formed at the loading slot 130 just below the support ring 210. The design of the recess further enhances the space utilization of the loading slot 130, providing additional accommodation for the sample. When the sample is injected into the sample injection slot 130 through the sample injection port 150 and the through cavity 220, the accommodating space of the groove can avoid the phenomenon that the added sample amount flows back and overflows due to too high speed and too high content.

The support assembly 200 can effectively support the sealing membrane 110, prevent the sealing membrane 100 from being irreversibly deformed under the action of external force, avoid the occurrence of deformation or collapse of the sealing membrane 110 possibly caused by the action of external force in the liquid injection process, avoid the failure of the sample adding groove 130, and ensure the structural integrity and the functional stability of the microfluidic chip in the use process.

Example 2

The main difference between this embodiment and embodiment 1 is the improved design of the support assembly 200, which is embodied by the increased use of the support sheet 240.

Referring to fig. 5, the support assembly 200 of the present embodiment includes a support plate 240 fixedly mounted on the chip substrate 100 in addition to the support ring 210 described in embodiment 1. The support piece 240 is disposed along the radian extending direction of the loading slot 130, one end of the support piece is close to the support ring 210, and the other end of the support piece extends to a communication point between the loading slot 130 and the microfluidic channel 140. So that the support 240 can cover a critical area of the loading slot 130, providing a wider support. The bottom of the supporting plate 240 is fixedly connected to the bottom of the sample loading slot 130, and the top is fixedly connected to the sealing membrane 110. The support plate 240 is disposed on the center dividing line of the loading slot 130, ensuring symmetry and stability of the structure.

The added support piece 240 can provide additional support for the whole sealing membrane 110, and effectively prevent the internal air pressure of the sealing membrane 110 from changing in the sample loading process, so that the non-sample loading port 150 is deformed or collapsed. The combined design of the support tabs 240 and the support ring 210 significantly enhances the structural strength of the entire support assembly 200. This enhancement not only improves the pressure resistance of the loading well 130, but also guides the flow direction of the sample to some extent.

Example 3

The main difference between this embodiment and embodiment 2 is the improved design of the support assembly 200, which is embodied by the reduced use of the support ring 210.

Referring to fig. 6, the support assembly 200 in this embodiment only includes a support plate 240 disposed along the radian extending direction of the loading slot 130, one end of the support plate is close to the support ring 210, and the other end of the support plate extends to the point where the loading slot 130 communicates with the microfluidic channel 140. So that the support 240 can cover a critical area of the loading slot 130, providing a wider support. The bottom of the supporting plate 240 is fixedly connected to the bottom of the sample loading slot 130, and the top is fixedly connected to the sealing membrane 110. The support plate 240 is disposed on the center dividing line of the loading slot 130, ensuring symmetry and stability of the structure.

By eliminating the support ring 210, the structure of the support assembly 200 is made more compact. The manufacturing cost is reduced, the process difficulty is reduced, and the production efficiency is improved.

The design of support tab 240, although eliminating support ring 210, still provides adequate support for sealing membrane 110. The two ends of the supporting plate 240 are respectively fixed on the bottom of the sample loading groove 130 and the sealing membrane 110, so that the stability of the sealing membrane 110 when being pressed is ensured, and the local deformation or collapse is prevented, thereby ensuring the stability and the continuity of the sample loading process.

The extended portion of the support plate 240 can provide a liquid flow guiding function to help the sample reach the connection point of the microfluidic channel 140 more quickly, reduce the residence time of the sample in the sample addition well 130, and improve the response speed and efficiency of the whole system.

Example 4

The main difference between this embodiment and embodiment 3 is the improved design of the support assembly 200, which is embodied by the structural shape of the support plate 240.

Referring to fig. 7, the support assembly 200 includes a plurality of support plates 240 for supporting the sealing membrane 110, and the plurality of support plates 240 are uniformly arranged along the extending direction of the loading slot 130. The sealing membrane 110 is ensured to be uniformly supported on the whole length of the sample adding groove 130, and deformation or damage caused by uneven local stress is avoided.

One end of each supporting plate 240 is fixedly connected to the chip substrate 100, namely, the bottom of the sample adding groove 130, and the other end of each supporting plate 240 is fixedly connected to the sealing membrane 110. This fixing ensures the stability of the support sheet 240 and the flatness of the sealing diaphragm 110.

The support plate 240 is arranged in an arch shape, that is, a space is left at the lower end of the middle of the support plate 240, so that a smooth flow path is provided for the sample. This not only reduces the fluctuation of the liquid level, but also ensures the uniform distribution of the sample in the sample addition groove 130, and improves the accuracy and consistency of the detection result.

Example 5

The main difference between this embodiment and embodiment 1 is the improved design of the support assembly 200, which is embodied by the structural shape of the support ring 210.

Referring to fig. 8, the support assembly 200 in this embodiment includes a support ring 210 fixedly mounted on the chip substrate 100, and the outer circumferential sidewall of the support ring 210 is fixedly connected to the sidewall of the loading slot 130, so as to ensure a stable connection between the support ring 210 and the loading slot 130. The bottom of the supporting ring 210 is spaced apart from the bottom of the sample loading slot 130, and the top is fixedly connected to the sealing membrane 110. This design not only ensures structural robustness, but also provides room for sample flow.

By fixedly attaching the outer circumferential side wall of the support ring 210 to the side wall of the loading slot 130, this manner of attachment ensures a secure connection between the support ring 210 and the loading slot 130. The support ring 210 remains stable even under external pressure or vibration, avoiding the risk of loosening or deformation of the structure.

The center of the supporting ring 210 is provided with a through cavity 220 in a penetrating way, and the top end of the through cavity 220 corresponds to and is communicated with the sample inlet 150 on the sealing membrane 110. The bottom of the through cavity 220 is also communicated with the bottom of the sample adding groove 130, so that the sample is smoothly added into the through cavity 220 through the sample adding port 150, and then flows into the bottom of the sample adding groove 130 from the bottom of the through cavity 220 and flows along the extending direction of the sample adding groove 130.

The central through cavity 220 of the support ring 210 is designed to provide a well-defined flow path for the sample. The sample enters the through cavity 220 through the sample inlet 150, flows into the bottom of the sample adding groove 130 from the bottom end of the through cavity 220, and flows smoothly along the extending direction of the sample adding groove 130. The design reduces liquid level fluctuation, ensures uniform distribution of samples and improves accuracy of detection results.

Example 6

The main difference between this embodiment and embodiment 1 is the improved design of the support assembly 200, which is embodied by the structural shape of the support ring 210.

Referring to fig. 9, the support assembly 200 in this embodiment includes a support ring 210 fixedly mounted on the chip substrate 100, wherein the bottom end of the support ring 210 is fixedly connected to the bottom of the sample loading slot 130, and the top end of the support ring is fixedly connected to the sealing membrane 110, so as to ensure structural stability. And the supporting ring 210 is disposed at one end of the sample loading slot 130 away from the communication point of the microfluidic channel 140, so as to avoid interference to the microfluidic channel 140 during the sample loading process and ensure the unobstructed flow path of the sample.

The center of the supporting ring 210 is provided with a through cavity 220 in a penetrating way, and the top end of the through cavity 220 corresponds to and is communicated with the sample inlet 150 on the sealing membrane 110. This design allows the loading gun or liquid sample holding tube to smoothly load sample into the through-cavity 220 through the loading port 150 and to guide the sample into the interior of the loading slot 130.

The sidewall of the support ring 210 is provided with a notch 230 facing the sample loading slot 130, the notch 230 is arranged along the direction perpendicular to the cavity 220, and the notch 230 is also arranged at the bottom end of the support ring 210 to be communicated with the sample loading slot 130. When the sample gun or the liquid sample holding tube injects the sample into the sample loading slot 130 through the sample loading port 150, the sample flows out of the notch 230 through the through cavity 220 and into the sample loading slot 130.

The central through cavity 220 of the support ring 210 is designed such that the sample can smoothly flow from the sample loading port 150 into the through cavity 220 and then out of the side wall gap 230 into the sample loading slot 130. This perpendicular arrangement of the through cavity 220 and the gap 230 reduces turbulence and swirling of the liquid during flow. When the sample enters the notch 230 from the through cavity 220, the flow in the vertical direction reduces the transverse disturbance, so that the liquid flow is more stable, the formation of bubbles is reduced, the accuracy judgment of the sample addition result is facilitated, and the interference caused by the bubbles is avoided.

In summary, the support assembly 200 of the present utility model not only solves various problems that may occur during the sample loading process, such as deformation of the sealing membrane 110, sample overflow, etc., but also improves the precision and stability of sample loading, thereby laying a solid foundation for efficient and reliable biological sample detection.

The foregoing is merely illustrative of the present utility model, and the present utility model is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.

Claims (10)

1. A microfluidic chip, comprising:

A chip substrate (100) provided with a sample loading groove (130) for temporarily storing samples;

A sealing membrane (110) covering the chip substrate (100) and provided with a sample adding port (150), wherein the sample adding port (150) is communicated with the sample adding groove (130) and is used for injecting a sample into the sample adding groove (130);

The plurality of detection holes (120) are uniformly distributed along the circumferential direction of the chip substrate (100) and are used for accommodating samples to be detected;

a microfluidic channel (140) for communicating the sample addition well (130) with the detection hole (120);

The sample adding groove (130) is formed along the upper surface of the chip substrate (100) in an extending way, the whole chip substrate is in a prolate groove shape, and the substrate (100) and the sealing membrane (110) form the sample adding groove (130).

2. The microfluidic chip according to claim 1, wherein a support assembly (200) is provided, the support assembly (200) comprises a support ring (210) mounted on the chip substrate (100) and used for supporting the sealing membrane (110), the outer circumferential side wall of the support ring (210) is fixedly connected to the side wall of the sample adding groove (130), the center of the support ring (210) is provided with a through cavity (220) in a penetrating manner, the sample adding port (150) is communicated with the upper end of the through cavity (220), and the lower end of the through cavity (220) is communicated with the sample adding groove (130).

3. The microfluidic chip according to claim 1, wherein a supporting component (200) is provided, the supporting component (200) comprises a supporting ring (210) installed on the chip substrate (100) and used for supporting the sealing membrane (110), one end of the supporting ring (210) is abutted to the bottom of the sample adding groove (130) and fixedly connected to the chip substrate (100), the other end of the supporting ring (210) is abutted to the sealing membrane (110), a through cavity (220) is penetrated in the center of the supporting ring (210), a notch (230) communicating with the through cavity (220) is formed in the bottom end of the supporting ring (210) along the direction perpendicular to the through cavity (220), the sample adding port (150) is communicated with the upper end of the through cavity (220), and the sample adding groove (130) is communicated with the through cavity (220) through the notch (230).

4. The microfluidic chip according to claim 1, wherein a supporting component (200) is provided, the supporting component (200) comprises a supporting ring (210) installed on the chip substrate (100) and used for supporting the sealing membrane (110), one end of the supporting ring (210) is abutted to the bottom of the sample adding groove (130) and fixedly connected to the chip substrate (100), the other end of the supporting ring (210) is abutted to the sealing membrane (110), the supporting ring (210) is arranged in a semicircular ring shape, a through cavity (220) is formed in the center of the supporting ring (210) in a penetrating mode, a notch (230) for communicating with the through cavity (220) is formed in the circumferential side face of the supporting ring (210) along the penetrating direction of the through cavity (220), the sample adding port (150) is communicated with the upper end of the through cavity (220), and the sample adding groove (130) is communicated with the through the notch (230).

5. The microfluidic chip according to claim 4, wherein the notch (230) expands radially outwardly, and an inner port distance of the notch (230) is smaller than an outer port distance of the notch (230).

6. A microfluidic chip according to any of claims 2-4, wherein the top surface of the support ring (210) is fixedly connected to the sealing membrane (110).

7. The microfluidic chip according to claim 6, wherein the support assembly (200) further comprises a plurality of support plates (240) uniformly arranged along the extending direction of the sample loading groove (130), one end of the support plate (240) is fixedly connected to the chip substrate (100), and the other end of the support plate (240) is fixedly connected to the sealing membrane (110).

8. The microfluidic chip according to claim 6, wherein the support assembly (200) further comprises a support plate (240) disposed along the extending direction of the loading slot (130), and the support plate (240) is disposed in an arc shape and is located in the middle of the loading slot (130).

9. The microfluidic chip according to claim 1, wherein the sample addition groove (130) extends in an arc shape from the sample addition port (150), and the sample addition groove (130) is disposed along a circumferential direction of the chip substrate (100).

10. A microfluidic chip according to claim 1, wherein the sealing membrane (110) is provided with a dosing line (250) for indicating the amount of added sample.