CN216955727U - A three-dimensional fluorescence detection device - Google Patents

Abstract

The utility model relates to a three-dimensional fluorescence detection device, which relates to the technical field of fluorescence detection and comprises a light source component, a sample accommodating component and a data detection component; the light source component is used for emitting exciting light to the sample accommodating component; the exciting light is first exciting light and second exciting light; the first exciting light is continuous light with periodically changed wavelength; the second exciting light is phase modulation high-frequency pulse light carrying a reference phase modulation signal; the data detection component is used for receiving the fluorescence of the first sample and the fluorescence of the second sample; the first sample fluorescence is generated after the first exciting light acts on the sample to be detected in the sample accommodating component; the fluorescence of the first sample comprises information representing the fluorescence intensity of the sample to be detected; the second sample fluorescence is generated after the second exciting light acts on the sample to be detected in the sample accommodating unit; the second sample fluorescence includes information characterizing the fluorescence lifetime of the sample to be detected. The utility model realizes more comprehensive detection of water pollution with low cost.

Description

A three-dimensional fluorescence detection device

technical field

The utility model relates to the technical field of fluorescence detection, in particular to a three-dimensional fluorescence detection device.

Background technique

Combining three-dimensional fluorescence spectrum detection with specific analysis algorithms is a widely used method for detecting and characterizing water quality in nature. Qualitative and quantitative analysis of water, so as to further determine the type and relative concentration of dissolved organic matter pollution in the water body.

Since the algal pigments and metabolites in natural water have fluorescent color reaction, the detection of heavy metal elements in water can be realized with specific probes. However, the literature and actual experimental data show that there is general noise in the fluorescence region of these two types of substances in natural water. Due to the characteristics of large peaks and small peaks, when using the parallel factor analysis and regional area integration in the traditional three-dimensional fluorescence spectrum detection method for qualitative and quantitative analysis, there are likely to be large errors, and the three-dimensional fluorescence spectrum may also have the problem that the characteristic peaks are too close to overlap.

Utility model content

The purpose of the utility model is to provide a three-dimensional fluorescence detection device to realize the multi-index detection of the sample to be tested.

For achieving the above object, the utility model provides the following scheme:

A three-dimensional fluorescence detection device, comprising a light source component, a sample accommodating component and a data detection component;

The light source component is used for emitting excitation light to the sample containing component; the excitation light is a first excitation light and a second excitation light; the first excitation light is continuous light whose wavelength changes periodically; the The second excitation light is a phase-modulated high-frequency pulse light carrying a reference phase-modulated signal;

The data detection component is used for receiving the fluorescence of the first sample and the fluorescence of the second sample;

The first sample fluorescence is the fluorescence generated after the first excitation light acts on the sample to be detected in the sample accommodating component; the first sample fluorescence includes information representing the fluorescence intensity of the sample to be detected;

The second sample fluorescence is the fluorescence generated after the second excitation light acts on the sample to be tested in the sample accommodating unit; the second sample fluorescence includes information representing the fluorescence lifetime of the sample to be detected.

Optionally, the light source assembly includes a first light source assembly and a second light source assembly; the first light source assembly is used for emitting first excitation light;

The second light source assembly includes a turntable, a first driving part and a second driving part;

A diaphragm and a lamp bead are uniformly arranged on the turntable; the diaphragm is used for emitting the first excitation light to the sample containing component; the lamp bead is used for driving the first driving component down, generating a second excitation light, and emitting the second excitation light to the sample accommodating component;

The second driving component is arranged on the turntable, and the second driving component is used to drive the turntable to rotate, so as to drive the diaphragm and the lamp beads on the turntable to rotate.

Optionally, the number of the lamp beads is three, and the lamp beads include a first lamp bead, a second lamp bead and a third lamp bead.

The first lamp bead is an algal protein fluorescent LED lamp bead, and the algal protein fluorescent LED lamp bead is used to generate the algal protein fluorescence carrying the first reference phase modulation signal;

The second lamp bead is an algae pigment fluorescent LED lamp bead, and the algae pigment fluorescent LED lamp bead is used to generate the algae pigment fluorescence carrying the second reference phase modulation signal;

The third lamp bead is a metal ion fluorescent LED lamp bead, and the metal ion fluorescent LED lamp bead is used to generate metal ion fluorescence carrying a third reference phase modulation signal.

Optionally, the first light source assembly includes a light source, a concave mirror, a filter wheel and a first monochromator;

the filter wheel is arranged at the entrance of the first monochromator;

The concave mirror, the light source and the filter wheel are located on the same axis, and the concave mirror is used for entering the light emitted by the light source into the first monochromator through the filter wheel;

The first monochromator is used for generating a first laser light according to the light emitted by the light source.

Optionally, the interval between the turntable and the first monochromator is 15 mm, and the diameter of the diaphragm is 5 mm.

Optionally, the three-dimensional fluorescence detection device further includes a light beam converging guide assembly;

The light beam condensing and guiding assembly includes an electric zoom lens, an excitation wave condensing lens, a beam splitter and a reference wave detector; the electric zoom lens, the excitation wave condensing lens and the beam splitter are sequentially arranged on the excitation light emission path;

The electric zoom lens is used to adjust the focal length of the excitation light, so that the excitation light is incident on the excitation wave condensing lens;

The beam splitter is used to obtain refracted excitation light and transmitted excitation light according to the converged excitation light output by the excitation wave condensing lens, emit the transmitted excitation light to the sample accommodating component, and transmit the transmitted excitation light to the sample accommodating component. refracted excitation light is emitted to the reference wave detector;

The reference wave detector is used for collecting the refracted excitation light, and converting the collected light signal into a first electrical signal; the first electrical signal is used for detecting the fluorescence intensity of the sample to be detected. in accordance with.

Optionally, the data detection assembly includes a first detection unit and a second detection unit;

The first detection unit and the sample accommodating component are arranged on the same horizontal axis, and the second detection unit and the sample accommodating component are arranged on the same vertical axis;

the first detection unit is configured to receive the fluorescence of the first sample in the horizontal direction;

The second detection unit is used for receiving the fluorescence of the first sample in the vertical direction and receiving the fluorescence of the second sample in the vertical direction.

Optionally, the first detection unit includes an aperture stop and an absorption detector;

The aperture diaphragm is used for emitting the fluorescence of the first sample to the absorption detector after performing a horizontal collimation constraint;

The absorption detector is used to collect the fluorescence of the first sample after the alignment constraint, and convert the collected optical signal into a second electrical signal; the second electrical signal is used to detect the fluorescence of the sample to be detected. The fluorescence intensity provides the basis.

Optionally, the second detection unit includes a fluorescent condensing lens group, a second monochromator and a linear CCD;

The fluorescent condensing lens group is used for emitting the fluorescence of the first sample or the fluorescence of the second sample to the second monochromator;

The second monochromator is used for grating diffraction of the first sample fluorescence or the second sample fluorescence, and then emitting to the linear CCD;

The linear array CCD is used for:

For the received fluorescence of the first sample, a multi-channel working mode is used to collect first optical signals at multiple frequencies, and convert the multiple first optical signals into multiple third electrical signals; the third electrical signals are To provide a basis for detecting the fluorescent light intensity of the sample to be detected;

For the received fluorescence of the second sample, a single-channel working mode is used to collect a second optical signal, and the second optical signal is converted into a fourth electrical signal; the fourth electrical signal is used to detect the to-be-detected sample The fluorescence lifetime of .

Optionally, the sample accommodating assembly includes a sample chamber, a stage and a cuvette;

The cuvette is placed on the stage, and the stage is arranged in the center of the sample chamber;

The cuvette is used for accommodating the sample to be tested.

According to the specific embodiments provided by the present utility model, the present utility model discloses the following technical effects:

The excitation light is emitted to the sample accommodating assembly through the light source assembly, so that the sample to be tested in the sample accommodating assembly is excited to generate fluorescence, and the data detection assembly receives the fluorescence generated by the sample to be tested. Wherein, when the excitation light is continuous light whose wavelength changes periodically, the data detection component receives fluorescence including information representing the fluorescence intensity of the sample to be tested; when the excitation light is a phase-modulated high-frequency pulsed light carrying a reference phase-modulated signal , the data detection component receives the fluorescence including information characterizing the fluorescence lifetime of the sample to be tested. The utility model realizes the collection of fluorescence light intensity information and lifetime information generated by the sample to be tested through a device, and achieves the purpose of multi-index detection of the sample to be tested (polluted water).

Description of drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only of the present invention. For some embodiments of the present invention, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without any creative effort.

1 is a schematic structural diagram of a three-dimensional fluorescence detection device of the present invention;

FIG. 2 is a schematic structural diagram of a turntable in the utility model.

Symbol Description:

1- Concave mirror, 2- Steady state xenon lamp, 3- Filter wheel, 4- First monochromator, 5- Turntable, 51- Diaphragm, 52- Algae protein fluorescent LED lamp beads, 53- Metal ion fluorescent LED Lamp beads, 54-algae pigment fluorescent LED lamp beads, 6-stepping motor, 7-LED driver module, 8-motorized zoom lens, 9-excitation wave converging lens, 10-beam splitter, 11-reference wave detector, 12-sample chamber, 13-stage, 14-cuvettes, 15-aperture diaphragm, 16-absorption detector, 17-fluorescence converging lens group, 18-second monochromator, 19-linear CCD, 20 - Microcomputer.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the implementations. example. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

The purpose of the utility model is to provide a three-dimensional fluorescence detection device, which combines the three-dimensional fluorescence detection optical path with the fluorescence lifetime detection optical path at low cost, and enhances the detection ability of algae and metal ions while realizing the traditional organic pollution detection of natural water bodies. , which enhances the ability to distinguish overlapping characteristic peaks in data collected by traditional 3D fluorescence spectrometers.

In order to make the above objects, features and advantages of the present utility model more clearly understood, the present utility model will be described in further detail below with reference to the accompanying drawings and specific embodiments.

As shown in FIG. 1 , the present invention provides a three-dimensional fluorescence detection device including a light source assembly, a sample accommodating assembly and a data detection assembly; the light source assembly is used to emit excitation light to the sample accommodating assembly; the The excitation light is a first excitation light and a second excitation light; the first excitation light is continuous light whose wavelength changes periodically; the second excitation light is a phase-modulated high-frequency pulse light carrying a reference phase modulation signal.

The data detection component is used for receiving the first sample fluorescence and the second sample fluorescence; the first sample fluorescence is the fluorescence generated after the first excitation light acts on the sample to be tested in the sample accommodating component; The first sample fluorescence includes information representing the fluorescence intensity of the sample to be detected; the second sample fluorescence is the fluorescence generated after the second excitation light acts on the sample to be detected in the sample accommodating unit; the The second sample fluorescence includes information characterizing the fluorescence lifetime of the sample to be detected.

The light source assembly includes a first light source assembly and a second light source assembly; the first light source assembly is used for emitting first excitation light; the second light source assembly includes a turntable 5 , a first driving part and a second driving part.

As shown in FIG. 2 , a diaphragm 51 and lamp beads are evenly arranged on the turntable 5 ; the diaphragm 51 is used for emitting the first excitation light to the sample accommodating component; the lamp beads are used for Under the driving of the first driving part, the second excitation light is generated, and the second excitation light is emitted to the sample accommodating component; the second driving part is arranged on the turntable 5, and the second excitation light is The second driving component is used to drive the turntable 5 to rotate, so as to drive the diaphragm 51 and the lamp beads on the turntable 5 to rotate.

Specifically, the second driving component is a stepper motor 6, and the turntable 5 is driven to rotate by the stepper motor 6, and is divided into four gear positions, and the rotation angle is fixed at 90° each time. The first driving component is an LED driving module 7, and the LED driving module 7 is used to add a reference phase modulation signal while providing a constant current source to the lamp bead.

The number of the lamp beads is three, and the lamp beads include a first lamp bead, a second lamp bead and a third lamp bead. The first lamp bead is an algal protein fluorescent LED lamp bead 52, and the algal protein fluorescent LED lamp bead 52 is used to generate algal protein fluorescence carrying a first reference phase modulation signal; the second lamp bead is an algal pigment fluorescence LED lamp bead 54, the algae pigment fluorescent LED lamp bead 54 is used to generate algae pigment fluorescence carrying the second reference phase modulation signal; the third lamp bead is a metal ion fluorescent LED lamp bead 53, the metal ion fluorescent lamp bead 53 The LED lamp beads 53 are used to generate metal ion fluorescence carrying the third reference phase modulation signal. The turntable 5 is specifically a heat dissipation disk. The above-mentioned three lamp beads and the diaphragm are evenly distributed on the heat dissipation disk at an interval of 90°, and the diaphragm 51 is facing the light outlet of the first monochromator 4 in the initial state;

Preferably, the diameter of the diaphragm is 5 mm, the wavelength of the algal protein fluorescent LED lamp bead 52 is 365 nm, the wavelength of the metal ion fluorescent LED lamp bead 53 is 470 nm, the wavelength of the algal pigment fluorescent LED lamp bead is 570 nm, and the above The power of the three lamp beads is 3W, and the frequency of the reference phase modulation signal provided by the LED driving module 7 is 1-9MHz.

The first light source assembly includes a light source, a concave mirror 1 , a filter wheel 3 and a first monochromator 4 . Specifically, the light source is a steady-state xenon lamp 2 .

The filter wheel 3 is arranged at the entrance of the first monochromator 4; the concave mirror 1, the light source and the filter wheel 3 are located on the same axis, and the concave mirror 1 is used for The light emitted by the light source enters the first monochromator 4 through the filter wheel 3; the first monochromator 4 is used to generate a first laser light according to the light emitted by the light source. Specifically, the concave mirror 1 guides the broad-spectrum light generated by the steady-state xenon lamp 2 into the filter wheel 3, and further into the excitation monochromator, and the distance between the excitation monochromator and the heat dissipation disk is 15mm .

The three-dimensional fluorescence detection device further includes a beam converging guide assembly; the beam converging guide assembly includes an electric zoom lens 8, an excitation wave condensing lens 9, a beam splitter 10 and a reference wave detector 11; the electric zoom lens 8 , the excitation wave condensing lens 9 and the beam splitter 10 are sequentially arranged on the emission light path of the excitation light.

The motorized zoom lens 8 is used to adjust the focal length of the excitation light, so that the excitation light is incident on the excitation wave condensing lens 9 ; After the excitation light is obtained, refracted excitation light and transmitted excitation light are obtained, the transmitted excitation light is emitted to the sample containing component, and the refracted excitation light is emitted to the reference wave detector 11; the reference wave The detector 11 is used to collect the refracted excitation light, and convert the collected light signal into a first electrical signal; the first electrical signal is used to provide a basis for detecting the fluorescence intensity of the sample to be detected.

Specifically, the transmission-reflection ratio of the beam splitter is 7:3. The electric zoom lens 8 has two fixed selection gears, respectively 150mm and 170mm. The electric zoom lens 8 introduces the phase-modulated light from the high-power LED or the steady-state light from the monochromator into the data detection component; the excitation The wave condensing lens 9 guides the excitation light output from the motorized zoom lens 8 to the beam splitter 10 , so that part of the excitation light is reflected, and the final convergence position of the reflected light is the reference wave detector 11 . The reference wave detector 11 converts the collected optical signal into an electrical signal, and then performs I/V conversion and amplification on the electrical signal through an integrated circuit to obtain a reference wave signal, so as to further standardize and correct the obtained fluorescence detection signal.

The data detection assembly includes a first detection unit and a second detection unit; the first detection unit and the sample accommodating assembly are arranged on the same horizontal axis, and the second detection unit is arranged with the sample accommodating assembly On the same vertical axis; the first detection unit is used for receiving the first sample fluorescence in the horizontal direction; the second detection unit is used for receiving the first sample fluorescence in the vertical direction, and receiving The second sample fluoresces in the vertical direction.

Further, the first detection unit includes an aperture stop 15 and an absorption detector 16; the aperture stop 15 is used to perform horizontal collimation constraints on the fluorescence of the first sample, and then emit to the absorption a detector 16; the absorption detector 16 is used to collect the fluorescence of the first sample after the alignment constraint, and convert the collected optical signal into a second electrical signal; the second electrical signal is used for detection The fluorescence intensity of the sample to be detected provides the basis. Preferably, the absorption detector 16 is a silicon photodiode.

Preferably, the second detection unit includes a fluorescent condensing lens group 17, a second monochromator 18 and a linear CCD 19; the fluorescent condensing lens group 17 is used to fluoresce the first sample or the second sample The fluorescence is emitted to the second monochromator 18 ; the second monochromator 18 is used for grating diffraction of the fluorescence of the first sample or the fluorescence of the second sample, and then emitted to the linear array CCD 19 .

The linear array CCD19 is used for:

For the received fluorescence of the first sample, a multi-channel working mode is used to collect first optical signals at multiple frequencies, and convert the multiple first optical signals into multiple third electrical signals; the third electrical signals are To provide a basis for detecting the fluorescent light intensity of the sample to be detected;

For the received fluorescence of the second sample, a single-channel working mode is used to collect a second optical signal, and the second optical signal is converted into a fourth electrical signal; the fourth electrical signal is used to detect the to-be-detected sample The fluorescence lifetime of .

The sample accommodating assembly includes a sample chamber 12 , an object stage 13 and a cuvette 14 ; the cuvette 14 is placed on the object stage 13 , and the object stage 13 is arranged in the sample chamber 12 the center; the cuvette 14 is used to accommodate the sample to be tested.

The specific working process of the three-dimensional fluorescence detection device provided by the present invention is as follows:

(1) The three-dimensional fluorescence detection device is initially in the three-dimensional fluorescence detection mode:

The diaphragm 51 on the heat dissipation disk 5 is facing the light outlet of the first monochromator 4; the concave mirror 1 condenses the wide-spectrum light of the steady-state xenon lamp 2, and enters the excitation monochromator 4 through the filter wheel 3, and excites the monochromator 4. The colorimeter 4 is in the scanning mode, and continuously outputs continuous light with a wavelength of 200 nm to 800 nm that changes periodically with a step size of 5 nm.

The continuous light whose wavelength changes periodically is constrained by the diaphragm 51, and the beam is shaped by the electric zoom lens 8 with a working focal length of 170mm and the excitation wave condensing lens 9, and is further reflected and polarized by the beam splitter 10 with a transmission reflectance ratio of 7:3. Part of the reference light is folded out; the reference wave detector 11 collects the refracted light to obtain the reference photocurrent signal and amplifies and converts it into a level signal for storage. The transmitted light enters the sample chamber 12 and converges at the center of the cuvette 14. The fluorescence is excited to obtain the first sample fluorescence; the first sample fluorescence carries the sample absorption spectrum information.

The light carrying the absorption spectrum information of the sample is constrained by the aperture diaphragm 15 to be a substantially collimated beam, and the photocurrent is collected by the opposite absorption detector 16 and amplified and converted into a level signal for storage. The absorbance was obtained by averaging the signal calculation.

Fluorescence detection is performed on the fluorescence of the first sample in the vertical direction of the excitation light of the sample to be tested. The fluorescence condensing lens group 17 guides the fluorescence into the emission monochromator, and is diffracted by the grating in the emission monochromator, so that the samples with different frequencies fluoresce After passing through the exit slit, it is distributed on the linear array CCD. At this time, the linear array CCD works in multi-channel mode; further, the microcomputer 20 is combined with the obtained absorbance signal to correct to obtain the real fluorescence intensity and record it as an intensity matrix. Preferably, by setting the scanning mode of the emission monochromator with a fixed step size, three-dimensional fluorescence spectrum detection and inner filter effect correction can be completed in this optical path.

(2) The three-dimensional fluorescence detection device is switched to the fluorescence lifetime detection state:

The heat dissipation disc 5 is driven by the stepping motor 6 to rotate 90°, and the LED driving module 7 provides the algae protein fluorescent LED lamp bead 52 with a constant current driving power supply and a 3MHz reference phase modulation signal, and the generated high-frequency pulses carrying the reference phase modulation signal The light is beam shaped by the electric zoom lens 8 with a working focal length of 150mm and the excitation wave condensing lens 9, and then enters the sample chamber 12 through the beam splitter 10 and converges at the center of the cuvette 14. The sample to be tested is subjected to fluorescence excitation to obtain the fluorescence of the second sample. ; The fluorescence phase signal of the second sample is carried in the fluorescence.

The emission light carrying the fluorescent phase signal enters the emission monochromator through the fluorescent condensing lens group 17 in the vertical direction, and the linear CCD 19 works in the single-channel mode, and transmits the collected electrical signals into the microcomputer 20, and the orthogonal reference contained in the microcomputer 20 The two quadrature reference signals generated by the signal generation module are simultaneously input to the filter module and the phase detection module to obtain the phase difference, and the fluorescence lifetime is obtained by the final calculation. Further, in the microcomputer 20, the reference signal can be made to be orthogonal to the phase shift of the fluorescence signals generated by different fluorescent substances, and the fluorescence signals of the corresponding substances can be eliminated to realize the identification function.

Driven by the stepping motor 6, the heat dissipation disc 5 can introduce the phase-modulated light generated by the metal ion fluorescent LED lamp bead 53 and the algae pigment fluorescent LED lamp bead 54 as the excitation light source into the sample chamber respectively for the fluorescence lifetime detection of the algae pigment and the metal ion. .

Compared with the prior art, the present utility model has the following beneficial effects:

(1) The utility model integrates a steady-state light source with a switchable phase modulation light source, and uses fluorescence lifetime detection to supplement the three-dimensional fluorescence detection, which can combine the three-dimensional fluorescence detection optical path with the fluorescence lifetime detection optical path at a low cost. The traditional organic pollution detection in natural water bodies enhances the detection ability of algae and metal ions, and realizes a more comprehensive detection of water body pollutants.

(2) The utility model enhances the ability to distinguish overlapping characteristic peaks in the data collected by the traditional three-dimensional fluorescence spectrometer by separately detecting the fluorescence intensity and fluorescence lifetime of the sample to be detected.

The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments, and the same and similar parts between the various embodiments can be referred to each other.

The principles and implementations of the present utility model are described herein using specific examples. The descriptions of the above embodiments are only used to help understand the method and the core idea of the present utility model; meanwhile, for those skilled in the art, according to The idea of the present utility model will have changes in the specific implementation and application scope. In conclusion, the content of this specification should not be construed as a limitation on the present invention.

Claims (10)

1. A three-dimensional fluorescence detection device, characterized in that, the three-dimensional fluorescence detection device comprises a light source assembly, a sample accommodating assembly and a data detection assembly; The light source component is used for emitting excitation light to the sample containing component; the excitation light is a first excitation light and a second excitation light; the first excitation light is continuous light whose wavelength changes periodically; the The second excitation light is a phase-modulated high-frequency pulse light carrying a reference phase-modulated signal; The data detection component is used for receiving the fluorescence of the first sample and the fluorescence of the second sample; The first sample fluorescence is the fluorescence generated after the first excitation light acts on the sample to be detected in the sample accommodating component; the first sample fluorescence includes information representing the fluorescence intensity of the sample to be detected; The second sample fluorescence is the fluorescence generated after the second excitation light acts on the sample to be tested in the sample accommodating unit; the second sample fluorescence includes information representing the fluorescence lifetime of the sample to be detected. 2 . The three-dimensional fluorescence detection apparatus according to claim 1 , wherein the light source assembly comprises a first light source assembly and a second light source assembly; the first light source assembly is used for emitting a first excitation light; 3 . The second light source assembly includes a turntable, a first driving part and a second driving part; A diaphragm and a lamp bead are uniformly arranged on the turntable; the diaphragm is used for emitting the first excitation light to the sample containing component; the lamp bead is used for driving the first driving component down, generating a second excitation light, and emitting the second excitation light to the sample accommodating component; The second driving component is arranged on the turntable, and the second driving component is used to drive the turntable to rotate, so as to drive the diaphragm and the lamp beads on the turntable to rotate. 3 . The three-dimensional fluorescence detection device according to claim 2 , wherein the number of the lamp beads is 3, and the lamp beads comprise a first lamp bead, a second lamp bead and a third lamp bead; 4 . The first lamp bead is an algal protein fluorescent LED lamp bead, and the algal protein fluorescent LED lamp bead is used to generate the algal protein fluorescence carrying the first reference phase modulation signal; The second lamp bead is an algae pigment fluorescent LED lamp bead, and the algae pigment fluorescent LED lamp bead is used to generate the algae pigment fluorescence carrying the second reference phase modulation signal; The third lamp bead is a metal ion fluorescent LED lamp bead, and the metal ion fluorescent LED lamp bead is used to generate metal ion fluorescence carrying a third reference phase modulation signal. 4. The three-dimensional fluorescence detection device according to claim 2, wherein the first light source assembly comprises a light source, a concave mirror, a filter wheel and a first monochromator; the filter wheel is arranged at the entrance of the first monochromator; The concave mirror, the light source and the filter wheel are located on the same axis, and the concave mirror is used for entering the light emitted by the light source into the first monochromator through the filter wheel; The first monochromator is used for generating a first laser light according to the light emitted by the light source. 5 . The three-dimensional fluorescence detection device according to claim 4 , wherein the interval between the turntable and the first monochromator is 15 mm, and the diameter of the diaphragm is 5 mm. 6 . 6 . The three-dimensional fluorescence detection device according to claim 1 , wherein the three-dimensional fluorescence detection device further comprises a beam convergence guide assembly; 6 . The light beam condensing and guiding assembly includes an electric zoom lens, an excitation wave condensing lens, a beam splitter and a reference wave detector; the electric zoom lens, the excitation wave condensing lens and the beam splitter are sequentially arranged on the excitation light emission path; The electric zoom lens is used to adjust the focal length of the excitation light, so that the excitation light is incident on the excitation wave condensing lens; The beam splitter is used to obtain refracted excitation light and transmitted excitation light according to the converged excitation light output by the excitation wave condensing lens, emit the transmitted excitation light to the sample accommodating component, and transmit the transmitted excitation light to the sample accommodating component. refracted excitation light is emitted to the reference wave detector; The reference wave detector is used for collecting the refracted excitation light, and converting the collected light signal into a first electrical signal; the first electrical signal is used for detecting the fluorescence intensity of the sample to be detected. in accordance with. 7. The three-dimensional fluorescence detection device according to claim 1, wherein the data detection component comprises a first detection unit and a second detection unit; The first detection unit and the sample accommodating component are arranged on the same horizontal axis, and the second detection unit and the sample accommodating component are arranged on the same vertical axis; the first detection unit is configured to receive the fluorescence of the first sample in the horizontal direction; The second detection unit is used for receiving the fluorescence of the first sample in the vertical direction and receiving the fluorescence of the second sample in the vertical direction. 8. The three-dimensional fluorescence detection device according to claim 7, wherein the first detection unit comprises an aperture diaphragm and an absorption detector; The aperture diaphragm is used for emitting the fluorescence of the first sample to the absorption detector after performing a horizontal collimation constraint; The absorption detector is used to collect the fluorescence of the first sample after the alignment constraint, and convert the collected optical signal into a second electrical signal; the second electrical signal is used to detect the fluorescence of the sample to be detected. The fluorescence intensity provides the basis. 9 . The three-dimensional fluorescence detection device according to claim 7 , wherein the second detection unit comprises a fluorescence condensing lens group, a second monochromator and a linear CCD; 10 . The fluorescent condensing lens group is used for emitting the fluorescence of the first sample or the fluorescence of the second sample to the second monochromator; The second monochromator is used for grating diffraction of the first sample fluorescence or the second sample fluorescence, and then emitting to the linear CCD; The linear array CCD is used for: For the received fluorescence of the first sample, a multi-channel working mode is used to collect first optical signals at multiple frequencies, and convert the multiple first optical signals into multiple third electrical signals; the third electrical signals are To provide a basis for detecting the fluorescence intensity of the sample to be detected; For the received fluorescence of the second sample, a single-channel working mode is used to collect a second optical signal, and the second optical signal is converted into a fourth electrical signal; the fourth electrical signal is used to detect the to-be-detected sample provide the basis for the fluorescence lifetime. 10 . The three-dimensional fluorescence detection device according to claim 1 , wherein the sample accommodating component comprises a sample chamber, an object stage and a cuvette; 10 . The cuvette is placed on the stage, and the stage is arranged in the center of the sample chamber; The cuvette is used for accommodating the sample to be tested.

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