CN205133606U - Micro - sampling device of fence formula - Google Patents

Abstract

The utility model discloses a fence-type micro-sampling device, which comprises a culture dish (1), a mesh fence (2) and a cover plate (4), the culture dish (1) is in the shape of a rectangular vessel, The bottom is smooth; the mesh fence (2) is composed of 8×12 cubes or 8×12×4 triangular prisms or 8×12×8 triangular prisms, and the outer edge of the mesh fence (2) is connected to the petri dish (1) The internal dimensions of the container body (3) are the same. The application of the device of the utility model can not only solve the problems of time-consuming, labor-intensive and poor accuracy of the traditional separation method, but also provide a novel sampling device for the single-cell (or single-spore) separation method.

Description

A fence-type micro-sampling device

technical field

The utility model belongs to the field of biological instruments, in particular to a fence-type micro-sampling device.

Background technique

Common microbial separation and purification methods include solid culture separation and purification and liquid culture separation and purification. Solid culture separation and purification include dilution pour plate method, coating plate method, plate streaking method, dilution shaking plate method, etc.; separation and purification with liquid medium is commonly done by dilution method.

Whether it is a solid separation and purification method or a liquid separation and purification method, it is an important disadvantage that only the species that are quantitatively dominant in the mixed microbial population can be separated. To obtain a single colony, it is necessary to repeat the activation of the strain, the proliferation culture, the purification culture, the identification of the isolated strain, the isolation and culture of the pure strain, etc. until a single colony appears on the plate. Therefore, this method has a large workload and It is time-consuming and labor-intensive, and a new method is urgently needed to make up for its many deficiencies. Single-cell (or single-spore) separation method can adopt micro-separation method to directly separate single cells or spores from mixed populations for culture to obtain pure culture. This method can simplify the separation and purification steps, and has high efficiency and high accuracy. However, the single-cell separation method has relatively high requirements for operating technology, and lacks a special micro-sampling device. Usually, the properly diluted sample is prepared into small droplets and dropped on a glass slide for observation under a microscope. Only one cell is selected. There is a lot of uncertainty in this method. If the collected droplet does not contain microorganisms, it is necessary to take the droplet again. If it contains multiple microorganisms, how to get a single droplet during the sampling process? Individuals have certain difficulties.

Utility model content

The purpose of this utility model is to overcome the above disadvantages and provide a fence-type microscope that can not only simplify the complex, cumbersome and time-consuming shortcomings of traditional separation, but also make up for the shortcomings of single-cell culture separation method droplet observation method. sampling device.

The purpose of this utility model is achieved in the following ways:

A fence-type micro-sampling device, including a petri dish, a mesh fence and a cover plate, the petri dish is in the shape of a rectangular vessel with a smooth bottom; the mesh fence consists of 8×12 cubes or 8×12×4 Triangular prisms or 8×12×8 triangular prisms. The outer edge of the mesh fence is the same as the inner size of the container body of the culture dish, especially the height of the mesh fence is consistent with the inner height of the container body of the culture dish.

The upper and lower mesh fences are all bottomless, and they are all open structures.

Arabic numerals are arranged horizontally corresponding to 8×12 square grids on the edge of the petri dish, and English letters are arranged vertically. The utility model is provided with symbols of Arabic numerals and English letters on the edge of the culture dish, which can be precisely positioned under a microscope and improves the accuracy of sampling.

Each square of the 8×12 mesh fence is divided by an “×” shape, which is equivalent to dividing a square into four triangles to form an 8×12×4 mesh fence.

Or the middle of each square of 8×12 mesh fence is divided by “×”, which is equivalent to dividing a square into four triangles to become 8×12×4 mesh fence; the middle of each square of 8×12×4 mesh fence It is divided by the shape of "ten", which is an 8×12×8 mesh fence. The mesh fence of the utility model has various specifications and can be selected according to needs.

The petri dish and the mesh fence are independent, can be installed together when in use, and can be disassembled after use. Both the petri dish and the mesh fence are reusable. Petri dishes, mesh fences, and covers are optically clear pure polystyrene petri dishes, mesh fences, and covers, respectively. The inner side of the cover plate is provided with a columnar protrusion matching the inner wall of the cube or triangular prism.

The sampling device used in the prior art of the single cell (or single spore) separation method is a glass slide, and the operation process is to prepare a small liquid droplet on the glass slide for observation under a microscope by appropriately diluting the sample. The liquid of one cell is used for pure culture. There are great uncertainties in the method used. If the collected droplet does not contain microorganisms, it is necessary to take the droplet again. If it contains multiple microorganisms, what happens in the sampling process? There are certain difficulties in obtaining a single individual. The utility model of this device can overcome the above-mentioned shortcomings. When in use, put this device on an inverted microscope, take different concentrations of strain dilutions and put them into the petri dishes of this device, put them into fences of different specifications, and cover them. Plate, observe with the aid of a microscope, look for single cells in the grid, you can replace different fences according to the situation of looking for single cells, use capillary, micro sampler, 10μL pipette tip or micro hook, needle, The ring etc. absorb the required single bacteria or spores for cultivation.

Due to the large sampling volume of this device (the volume of this device is about 10mL) and the variety of fences, the possibility of finding single cells is very high. The application of this device can greatly simplify the separation and purification steps and improve efficiency. Operating under visual conditions can improve the accuracy of pure culture of microorganisms.

Compared with the prior art, the utility model has the beneficial effects: the application of the device can not only solve the problems of time-consuming, labor-intensive and poor accuracy of the traditional separation method, but also provide a new sampling device for the single-cell (or single-spore) separation method.

Description of drawings

Fig. 1 is a side view of a fence-type micro-sampling device 1 of the present invention.

In the figure, 1 is a petri dish, 2 is a mesh fence, 3 is a container body, 4 is a cover plate, and 5 is a protrusion.

Figure 2 is a schematic diagram of a petri dish.

Figure 3 is a schematic diagram of a mesh fence.

Figure 4 is a schematic diagram of the cover plate.

Figure 5 is a picture of a petri dish equipped with an 8×12 mesh fence.

Figure 6 is a diagram of a Petri dish equipped with 8×12×4 mesh fences.

Figure 7 is a diagram of a petri dish equipped with 8*12*8 mesh fences.

detailed description

The utility model is further described below by specific embodiment:

As shown in Fig. 1-7, a kind of fence type microscopic sampling device comprises petri dish 1, mesh fence 2 and cover plate 4, and described petri dish 1 is rectangular vessel shape, and bottom is smooth; Mesh fence 2 It consists of 8×12 cubes or 8×12×4 triangular prisms or 8×12×8 triangular prisms. The outer edge of the mesh fence 2 is the same as the inner size of the container body 3 of the culture dish 1 .

Mesh fence 2 is all bottomless up and down, and is an open structure.

Arabic numerals are arranged horizontally corresponding to the 8×12 square grids on the edge of the petri dish 1, and English letters are arranged vertically.

Each square of the 8×12 mesh fence is divided by an “×” shape, which is equivalent to dividing a square into four triangles to form an 8×12×4 mesh fence.

Or the middle of each square of 8×12 mesh fence is divided by “×”, which is equivalent to dividing a square into four triangles to become 8×12×4 mesh fence; the middle of each square of 8×12×4 mesh fence It is divided by the shape of "ten", which is an 8×12×8 mesh fence.

The petri dish 1 and the mesh fence 2 are independent of each other. The culture dish 1, the mesh fence 2 and the cover plate 4 are optically transparent pure polystyrene culture dish, the mesh fence and the cover plate respectively. The inner side of the cover plate 4 is provided with a columnar protrusion 5 matching the inner wall of a cube or a triangular prism.

Claims (6)

1. A fence type micro-sampling device, characterized in that it comprises a petri dish (1), a mesh fence (2) and a cover plate (4), the petri dish (1) is a rectangular container with a smooth bottom, The mesh fence (2) is composed of 8×12 cubes or 8×12×4 triangular prisms or 8×12×8 triangular prisms, and the outer edge of the mesh fence (2) is connected to the container body of the petri dish (1) ( 3) The internal dimensions are the same. 2. The fence-type micro-sampling device according to claim 1, characterized in that the mesh fence (2) has no bottom at all up and down, and is an open structure. 3. The fence-type micro-sampling device according to claim 1, characterized in that the middle of each square of the 8×12 mesh fence (2) is divided by an “×”, which is equivalent to dividing a square into four Triangular, become 8 x 12 x 4 mesh fence. 4. The fence-type micro-sampling device according to claim 1, characterized in that the middle of each square of the 8×12 mesh fence (2) is divided by an “×”, which is equivalent to dividing a square into four The triangle becomes an 8×12×4 mesh fence; the middle of each square of the 8×12×4 mesh fence is divided by a “ten” shape, which is an 8×12×8 mesh fence. 5. The fence-type micro-sampling device according to claim 1, characterized in that the petri dish (1), mesh fence (2) and cover plate (4) are optically transparent pure polystyrene petri dish, Mesh fencing and decking. 6. The fence-type micro-sampling device according to claim 1, characterized in that the inner side of the cover plate (4) is provided with a columnar protrusion (5) that coincides with the inner wall of a cube or a triangular prism.